AT4G28310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014299
Physical Location & Seq
Forward (+)
14017343 .. 14018212
870 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28310.1

Sequence Viewer

Length: 609 bp
ATGATGACGCCAGTGAAGAAAAGTATCGCCATTAGACCGGTGTCGTTCTACGGAAACGGGCTTCCTCGTCCTCGTTTCTTCGATAATCCTAAGTTCAATGATTACCGTGTTGATCCTCCCCTCTCTGTGCTAGATCCTCTTCTCTCATGGGCGAGAGACGCTCACTGGTCAATGGGTGGTCTCAACTTCACGCGTCTCCGTCTCCAAGGCCGGATCGAAGGAAACGTCAACAAGCTCCGTGCACAGCTTGAGAAATCCACCCCTGTGAAACTCGAATCTGGAAGGAAGAAGAAGAGATCTGGTTCTGAGTCTCCTCCTTCTGCTCCGATTGTGGTGAAACGAAGGAGGTACTTAGATCTGAACGATTCTGATGATGAGGAAGTCGGATCTGAAGATGAAGGTGTGGTTAGAATCAGGAGAAAACTCTCTGATGATTTTGATAGAGTCGCTGAAGAAAGCAAGACTAAACTTGTTGAAGCTAACAAGAAATCGATTAAATCTGAGTTTGTGGAGAAGAAGAGATTGATTGAGAAGAAGAAGATCGTAAAAGTTAACAAGACAAGTTCAACGAGGTCATCTCCAAGACTGGCTAAGCGTAGCTCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

23.08

Weight (kDa)

10.27

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52270 AT4G28310
fragaria_vesca FvH4_6g26250
malus_domestica MD17G1239900.v1.1
prunus_persica Prupe.3G132500_v2.0.a1
pyrus_communis pycom09g16430 pycom17g24320
rosa_chinensis RchiOBHm_Chr2g0126621
rosa_laevigata RLG00000018914
rosa_multiflora Rmu_sc0002369.1_g000021 Rmu_sc0007463.1_g000014
rosa_roxburghii Rroxscaffold_2G00117070
rosa_rugosa Rorug02G0264000
rosa_samantha Rh2AG321000 Rh2BG329300 Rh2DG348700
rosa_wichuraiana Rw2G026010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 193
AclWI GGATC 4 cut(s) 107, 128, 221, 394
AcuI CTGAAG 2 cut(s) 411, 471
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 191
AgeI ACCGGT 1 cut(s) 37
AgsI TTSAA 3 cut(s) 97, 476, 567
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 4 cut(s) 235, 247, 479, 600
AluI AGCT 4 cut(s) 235, 247, 479, 600
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 5 cut(s) 150, 185, 200, 206, 315
Alw44I GTGCAC 1 cut(s) 240
AlwI GGATC 4 cut(s) 107, 128, 221, 394
AoxI GGCC 1 cut(s) 208
ApaLI GTGCAC 1 cut(s) 240
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
AsiGI ACCGGT 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 346
BaeGI GKGCMC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 244
BcoDI GTCTC 5 cut(s) 150, 185, 200, 206, 315
BfaI CTAG 1 cut(s) 131
BglII AGATCT 2 cut(s) 296, 355
BlpI GCTNAGC 1 cut(s) 591
BoxI GACNNNNGTC 1 cut(s) 40
BplI GAGNNNNNCTC 4 cut(s) 145, 177, 562, 594
Bpu1102I GCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 37
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 3 cut(s) 11, 170, 591
BseCI ATCGAT 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 297, 492
BseNI ACTGG 3 cut(s) 11, 170, 591
BseRI GAGGAG 1 cut(s) 303
BseSI GKGCMC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 193
BshFI GGCC 1 cut(s) 210
BshTI ACCGGT 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 2 cut(s) 38, 211
BsmAI GTCTC 5 cut(s) 150, 185, 200, 206, 315
BsmBI CGTCTC 3 cut(s) 150, 200, 206
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
Bsp1720I GCTNAGC 1 cut(s) 591
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 2 cut(s) 298, 493
BspDI ATCGAT 1 cut(s) 491
BspFNI CGCG 1 cut(s) 193
BspPI GGATC 4 cut(s) 107, 128, 221, 394
BspTNI GGTCTC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 3 cut(s) 11, 170, 591
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 3 cut(s) 144, 287, 512
BstACI GRCGYC 1 cut(s) 8
BstDEI CTNAG 5 cut(s) 90, 306, 352, 501, 591
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 7 cut(s) 115, 136, 216, 299, 358, 389, 543
BstMAI GTCTC 5 cut(s) 150, 185, 200, 206, 315
BstMBI GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstPAI GACNNNNGTC 1 cut(s) 40
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 1 cut(s) 193
BstX2I RGATCY 4 cut(s) 133, 296, 355, 386
BstYI RGATCY 4 cut(s) 133, 296, 355, 386
Bsu15I ATCGAT 1 cut(s) 491
BsuRI GGCC 1 cut(s) 210
BsuTUI ATCGAT 1 cut(s) 491
BtsIMutI CAGTG 2 cut(s) 18, 163
Cfr10I RCCGGY 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 491
CseI GACGC 3 cut(s) 16, 167, 182
Csp6I GTAC 1 cut(s) 349
CspAI ACCGGT 1 cut(s) 37
CviAII CATG 1 cut(s) 147
CviJI RGCY 7 cut(s) 61, 210, 235, 247, 479, 590, 600
CviKI_1 RGCY 7 cut(s) 61, 210, 235, 247, 479, 590, 600
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 5 cut(s) 90, 306, 352, 501, 591
DpnI GATC 7 cut(s) 114, 135, 215, 298, 357, 388, 542
DpnII GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
Eam1104I CTCTTC 3 cut(s) 144, 287, 512
EarI CTCTTC 3 cut(s) 144, 287, 512
Eco130I CCWWGG 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 411, 471
EcoT14I CCWWGG 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 205
Esp3I CGTCTC 3 cut(s) 150, 200, 206
FaeI CATG 1 cut(s) 150
FaiI YATR 1 cut(s) 148
FatI CATG 1 cut(s) 146
FspBI CTAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 210
HapII CCGG 2 cut(s) 38, 211
HgaI GACGC 3 cut(s) 16, 167, 182
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 1 cut(s) 150
HincII GTYRAC 2 cut(s) 229, 553
HindII GTYRAC 2 cut(s) 229, 553
HinfI GANTC 5 cut(s) 275, 308, 365, 411, 444
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 2 cut(s) 38, 211
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 3 cut(s) 229, 242, 553
Hpy188I TCNGA 8 cut(s) 307, 327, 360, 370, 386, 391, 430, 502
Hpy188III TCNNGA 2 cut(s) 279, 415
Hpy8I GTNNAC 3 cut(s) 229, 242, 553
HpyAV CCTTC 5 cut(s) 212, 276, 327, 336, 392
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 5 cut(s) 90, 306, 352, 501, 591
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
LmnI GCTCC 2 cut(s) 240, 328
LpnPI CCDG 9 cut(s) 24, 51, 151, 224, 264, 276, 285, 400, 572
MaeI CTAG 1 cut(s) 131
MaeII ACGT 1 cut(s) 225
MalI GATC 7 cut(s) 114, 135, 215, 298, 357, 388, 542
MboI GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
MflI RGATCY 4 cut(s) 133, 296, 355, 386
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 1 cut(s) 604
MluI ACGCGT 1 cut(s) 191
MlyI GAGTC 2 cut(s) 317, 453
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 9 cut(s) 75, 81, 126, 131, 147, 324, 339, 370, 564
MseI TTAA 2 cut(s) 495, 552
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 2 cut(s) 38, 211
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
NlaIII CATG 1 cut(s) 150
OliI CACNNNNGTG 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 3 cut(s) 275, 365, 411
PinAI ACCGGT 1 cut(s) 37
PleI GAGTC 2 cut(s) 316, 452
PpsI GAGTC 2 cut(s) 316, 452
PshAI GACNNNNGTC 1 cut(s) 40
PsuI RGATCY 4 cut(s) 133, 296, 355, 386
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 2 cut(s) 495, 552
Sau3AI GATC 7 cut(s) 112, 133, 213, 296, 355, 386, 540
SchI GAGTC 2 cut(s) 317, 453
SduI GDGCHC 1 cut(s) 244
SetI ASST 8 cut(s) 228, 237, 249, 350, 403, 481, 575, 602
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 1 cut(s) 604
SspMI CTAG 1 cut(s) 131
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 1 cut(s) 228
TaqI TCGA 4 cut(s) 81, 216, 273, 491
TasI AATT 1 cut(s) 604
TfiI GAWTC 3 cut(s) 275, 365, 411
Tru1I TTAA 2 cut(s) 495, 552
Tru9I TTAA 2 cut(s) 495, 552
TscAI CASTG 2 cut(s) 18, 170
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 3 cut(s) 66, 188, 227
TspRI CASTG 2 cut(s) 18, 170
VneI GTGCAC 1 cut(s) 240
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.