Rorug02G0264000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014299
Physical Location & Seq
Reverse (-)
27866159 .. 27870685
4527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0264000.1

Sequence Viewer

Length: 3564 bp
ATGAAACCCCACAAACCCATTTCACACCACCACTTCTTTCTTCTTCTTCTTCTTCTCTTCTTGCTCTCTGCAACTCCCTCTTCTTCTTCTTCTTCATCAGCTTACAAAGACGCCCAGCAACTCCTGAGCTTCAAAAACTCTCTTCCGAAACCTACCCTTCTCTCCAATTGGCTCCCTGACCAAAACCCATGTCTGTTTTCCGGCGTTTTCTGCAAACAAACCCGGGTTTCCTCCATAGACCTGAGCTCCGTCCCCTTGTCCACAAACCTCACAATTATCTCCACTTTCTTGATGACCATTGATTCTCTACAATCACTCACTCTGAAATCTACCAGCCTCACCGGCCCCATTTCTTTTCCGGCTAAATCCAAGTGCAGCCCTCTCCTCACTTACATAGATCTAGCTCAGAACAGCTTGTCCGGCCCAATTTCCGGTATTCCCAACTTGGCTTCCTGCTCGAGCTTGAACTCTCTTAACCTCTCCACCAACCTCCTCGAGTTCAATGCCAAGGACTCGGCCACGTTCAGACTCTCCCTCCAGGTCCTCGACCTCTCCTTCAACAAGATTGCCGGCCCCGCCGTGCCGTGGATCGTATCCGACGGCTGCAGCGAGCTTGTAAAACTAGTTCTCAAGGGGAACAAGATCACCGGCGACATGAGTGTCTCTGGGTGCAAGAAGCTCGAGACTCTCGACTTCTCCTCCAACAATTTCACCGTGGAGATTCCGTCTTTCGGGGACTGTTTGGTTCTGGACCATCTCGACATCTCCGGCAACAAATTCTCCGGCGACGTCGCGCATGCTCTCTCTTCCTGCAAGCAGCTGACGTTTCTGAACCTCTCCAGAAACCATTTCTCCGGTCCTATTCCGGCCATTCCCACCCAGAATCTTCAGTTTCTTTCCCTCTCCGGCAACGAGTTCAAAGGTGCAATTCCACCCAGCTTGCTCAGTTCGTGTGAGTCGCTAGTGGAGCTGGATCTTTCCATAAATAATCTCACGGGCACCGTTCCCGATGCATTGAGCTCTTGCGCTTCCTTGGAGTCTCTCGACATATCCAACAACTTCTTCACCGGTGAGTTGCCCATCGAAACTCTGTTGAAGCTCAGCAACCTAAAGAGTGTGTCTCTTTCTCTCAACGGTTTCTTTGGTACGCTGCCTCGCTCTCTCTCCGAACTTTCATATCTGGACAGCTTGGATCTGAGCTCGAACAATTTCACTGGCTCCATTCCGAGTTGGCTTTGCGAAGATCCCAGGAACAGCTGGAAGGAGCTGTACCTCCAGAACAATAAGTTCGGTGGTACTATTCCTCCAGCTCTCAGCAACTGTACTCAGCTCGTTGCTCTTGATTTGAGCTTCAATTACCTCACTGGTACCATCCCTGCCAGCTTGGGGTCTTTGTCTAAGCTTCGCGATTTGATCATTTGGTTGAATCAGCTTAGCGGGGAAATTCCCCAGGAGCTCATGAATCTTGGGTTGCTGGAGAATCTTATCCTGGACTTCAATGAGCTGACCGGGACGATTCCTGTGGGGCTGAGTAACTGCACCAATTTGAGCTGGATTTCGTTGGCGAATAACAAGTTGAGTGGTGAGATTCCGGCGTGGATTGGCAAGCTTCCGAAGCTGGCGATACTGAAGCTGAGTAACAACTCATTCTATGGGAACATTCCCCCGGAGCTGGGGGACTGTAAGAGCTTGATATGGTTGGATCTCAATACTAACTTGTTGAATGGTACAATCCCTCCTGGGCTTTTCAAGCAGTCTGGCAACATTGCGGTGAATTTTGTTGCTTCCAAGACGTATGTGTATATCAAGAATGATGGAAGCAAGGAGTGCCATGGAGCAGGGAATTTGCTGGAGTTTGCGGGGATAAGACAAGAGCAATTGACCAGGATTTCGACGAGGAACCCCTGCAACTTCACTAGAGTGTATAGAGGTATTCTCCAACCAACATTTAATGACAATGGGACGATGATTTTTCTTGATATCTCACACAATAGGTTATCGGGTAGCATTCCGAAAGAGATTGGGAGTATGTACTATCTCTATATTCTGAATCTGGGCCATAACAATATATCTGGAGCAATCCCACTAGAGCTTGGGAAATTGAAGGACCTCAACATTCTTGATCTCTCTAGCAACAGCCTTGATGGAAGTATTCCTCAGTCTTTGGTTAGCCTTTCCATGCTGATGGAGATCGATTTGTCAAACAATCATCTGTCTGGAATGATTCCAGATTCAGGCCAGTTTGAGACATTCCCTGCTTACAGATTTATGAACAATTCAGGCCTTTGTGGCTACCCTTTGAATTCCTGTGGAGATGCTTCTGGGGCAAATGGAAATGGGCACCAGAAATCTCATCGACAAGCGTCCCTGGCAGGCAGTGTGGCAATGGGGCTACTGTTTTCCCTTTTCTGCATCTTTGGTTTGCTTATTGTTGCAATTGAAACCAAGAAAAGGCGAAAAAAGAAGGATTCAGGTCTGGACGTTTATGTGGACAGTCGTTCCCACTCCGGTACTGCCTGGAAGCTAACGGGTGCCCGAGAAGCATTAAGCATCAACCTCTCCACATTTGAGAAGCCACTTCAAAAGCTCACTTTTGCAGATCTTCTTGAGGCTACCAATGGTTTCCACAATGACAGTCTTATAGGCTCTGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAGGATGGCAGCATTGTTGCCATCAAGAAGTTGATACATATCAGCGGGCAAGGTGATCGTGAATTCACTGCTGAAATGGAAACTATCGGGAAGATCAAGCACCGAAACCTTGTTCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTAGTTTACGAGTACATGAAGTATGGAAGCTTAGATGATGTTTTACATGACCAGAAGAAAGGTATCAAGCTGAGTTGGTCTGCAAGGAGGAAGATTGCGATTGGATCTGCGAGAGGACTGGCCTTTCTTCACCACAATTGCATTCCTCACATCATTCATAGGGACATGAAATCGAGCAACGTCCTGCTCGATGAGAATCTAGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACTCATTTGAGTGTGAGCACCCTGGCAGGCACACCTGGTTATGTTCCTCCAGAATACTACCAGAGTTTCAGATGTTCTACAAAAGGCGATGTTTATAGTTATGGTGTAGTATTGCTCGAGCTGCTAACAGGAAGACGTCCTACAGATTCAGCTGATTTTGGTGACAATAATCTTGTTGGTTGGGTGAAGCAACATGCTAAATTAAAAATAAGTGATGTTTTTGATCCAGAGCTCATGAAAGAGGACCCAACCCTTGAGATTGAGCTGTTACAGCACTTAAAGGTGGCTTGCGCTTGTTTGGATGATAGGCCATGGCGCCGCCCTACGATGATTCAAGTCATGGCGCAGTTCAAGGAAATCCAAGCAGGACCCGGGATGGACTCCCAATCAACCATAGCCACCGACGATGGAGGTTTTGGTGCAGTTGAAATGGTTGAGATGAGCATAAAAGAAGACCCTGAACCGGGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1187

Amino Acids

129.43

Weight (kDa)

6.17

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 71 1.7e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 226 - 284 8.9e-06 Leucine rich repeat
LRR_14 PF23598 240 - 476 5.5e-09 Leucine-rich repeat region
LRR_8 PF13855 295 - 353 2.6e-08 Leucine rich repeat
LRR_14 PF23598 436 - 570 5.9e-06 Leucine-rich repeat region
LRR_8 PF13855 492 - 549 4.9e-07 Leucine rich repeat
Island PF20141 587 - 651 8.7e-32 Brassinosteroid receptor island domain
LRR_8 PF13855 680 - 738 2.2e-06 Leucine rich repeat
Pkinase PF00069 875 - 1144 7.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 878 - 1144 1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52270 AT4G28310
fragaria_vesca FvH4_6g26250
malus_domestica MD17G1239900.v1.1
prunus_persica Prupe.3G132500_v2.0.a1
pyrus_communis pycom09g16430 pycom17g24320
rosa_chinensis RchiOBHm_Chr2g0126621
rosa_laevigata RLG00000018914
rosa_multiflora Rmu_sc0002369.1_g000021 Rmu_sc0007463.1_g000014
rosa_roxburghii Rroxscaffold_2G00117070
rosa_rugosa Rorug02G0264000
rosa_samantha Rh2AG321000 Rh2BG329300 Rh2DG348700
rosa_wichuraiana Rw2G026010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 659
AatII GACGTC 2 cut(s) 792, 3226
Acc65I GGTACC 1 cut(s) 1367
AccB1I GGYRCC 5 cut(s) 998, 1367, 2340, 2531, 3402
AccI GTMKAC 1 cut(s) 2667
AccII CGCG 2 cut(s) 794, 1407
AciI CCGC 6 cut(s) 576, 1437, 1769, 1859, 2727, 3406
AclWI GGATC 7 cut(s) 596, 981, 1200, 1238, 1710, 2947, 3305
AcoI YGGCCR 2 cut(s) 516, 867
AcsI RAATTY 6 cut(s) 776, 1443, 1774, 1843, 2302, 2744
AcuI CTGAAG 2 cut(s) 872, 1649
AcyI GRCGYC 4 cut(s) 111, 789, 3223, 3403
AfaI GTAC 9 cut(s) 1147, 1271, 1297, 1324, 1369, 1729, 2033, 2512, 2849
AgeI ACCGGT 1 cut(s) 1067
AhlI ACTAGT 1 cut(s) 622
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AleI CACNNNNGTG 1 cut(s) 1919
AloI GAACNNNNNNTCC 2 cut(s) 401, 433
Alw21I GWGCWC 6 cut(s) 248, 1022, 1202, 1458, 3107, 3321
Alw26I GTCTC 6 cut(s) 667, 677, 1044, 1125, 2240, 3052
AlwI GGATC 7 cut(s) 596, 981, 1200, 1238, 1710, 2947, 3305
AlwNI CAGNNNCTG 1 cut(s) 1320
Ama87I CYCGRG 7 cut(s) 222, 457, 494, 680, 2535, 3203, 3458
ApeKI GCWGC 7 cut(s) 375, 603, 606, 817, 1150, 2691, 3208
ApoI RAATTY 6 cut(s) 776, 1443, 1774, 1843, 2302, 2744
ArsI GACNNNNNNTTYG 4 cut(s) 175, 207, 1873, 1905
AsiGI ACCGGT 1 cut(s) 1067
Asp700I GAANNNNTTC 1 cut(s) 1208
Asp718I GGTACC 1 cut(s) 1367
AspLEI GCGC 5 cut(s) 796, 1028, 3380, 3405, 3433
AsuC2I CCSGG 7 cut(s) 223, 224, 1510, 1667, 3459, 3460, 3552
AvaI CYCGRG 7 cut(s) 222, 457, 494, 680, 2535, 3203, 3458
AvaII GGWCC 6 cut(s) 541, 751, 857, 2107, 3331, 3455
BaeGI GKGCMC 3 cut(s) 1001, 2343, 2536
BanI GGYRCC 5 cut(s) 998, 1367, 2340, 2531, 3402
BanII GRGCYC 5 cut(s) 248, 1022, 1202, 1458, 3321
BarI GAAGNNNNNNTAC 4 cut(s) 1253, 1285, 3211, 3243
BbsI GAAGAC 2 cut(s) 3226, 3546
Bbv12I GWGCWC 6 cut(s) 248, 1022, 1202, 1458, 3107, 3321
BbvI GCAGC 7 cut(s) 387, 590, 618, 829, 1137, 2703, 3195
BceAI ACGGC 4 cut(s) 563, 568, 616, 2846
BcgI CGANNNNNNTGC 6 cut(s) 485, 519, 661, 695, 2720, 2754
BciVI GTATCC 2 cut(s) 604, 2801
BclI TGATCA 1 cut(s) 1413
BcnI CCSGG 7 cut(s) 223, 224, 1510, 1667, 3459, 3460, 3552
BcoDI GTCTC 6 cut(s) 667, 677, 1044, 1125, 2240, 3052
BcuI ACTAGT 1 cut(s) 622
BfaI CTAG 7 cut(s) 401, 623, 962, 1917, 2087, 2130, 3035
BfmI CTRYAG 2 cut(s) 604, 3228
BfoI RGCGCY 1 cut(s) 3406
BfuI GTATCC 2 cut(s) 604, 2801
BglI GCCNNNNNGGC 2 cut(s) 342, 2289
BglII AGATCT 2 cut(s) 397, 2599
BisI GCNGC 8 cut(s) 376, 604, 607, 818, 1151, 2692, 3209, 3406
BlpI GCTNAGC 2 cut(s) 1100, 1433
BlsI GCNGC 8 cut(s) 377, 605, 608, 819, 1152, 2693, 3210, 3407
Bme18I GGWCC 6 cut(s) 541, 751, 857, 2107, 3331, 3455
BmeT110I CYCGRG 7 cut(s) 222, 457, 494, 680, 2535, 3203, 3458
BmsI GCATC 4 cut(s) 1000, 2305, 2421, 2559
BoxI GACNNNNGTC 1 cut(s) 2362
BpiI GAAGAC 2 cut(s) 3226, 3546
BplI GAGNNNNNCTC 4 cut(s) 1105, 1137, 1920, 1952
BpmI CTGGAG 8 cut(s) 521, 823, 1259, 1290, 1496, 1871, 2094, 3120
Bpu10I CCTNAGC 2 cut(s) 125, 242
Bpu1102I GCTNAGC 2 cut(s) 1100, 1433
BpuEI CTTGAG 3 cut(s) 614, 2627, 3362
BpuMI CCSGG 7 cut(s) 223, 224, 1510, 1667, 3459, 3460, 3552
Bsa29I ATCGAT 1 cut(s) 2193
BsaBI GATNNNNATC 3 cut(s) 2189, 2720, 3030
BsaHI GRCGYC 4 cut(s) 111, 789, 3223, 3403
BsaWI WCCGGW 4 cut(s) 431, 854, 1067, 2507
Bse118I RCCGGY 4 cut(s) 341, 569, 647, 1067
Bse1I ACTGG 4 cut(s) 1219, 1369, 2239, 2958
Bse3DI GCAATG 3 cut(s) 1764, 2391, 3087
Bse8I GATNNNNATC 3 cut(s) 2189, 2720, 3030
BseCI ATCGAT 1 cut(s) 2193
BseGI GGATG 4 cut(s) 1371, 2692, 3394, 3468
BseJI GATNNNNATC 3 cut(s) 2189, 2720, 3030
BseMI GCAATG 3 cut(s) 1764, 2391, 3087
BseNI ACTGG 4 cut(s) 1219, 1369, 2239, 2958
BseRI GAGGAG 3 cut(s) 374, 482, 688
BseSI GKGCMC 3 cut(s) 1001, 2343, 2536
BseXI GCAGC 7 cut(s) 387, 590, 618, 829, 1137, 2703, 3195
BseYI CCCAGC 3 cut(s) 114, 935, 1672
BsgI GTGCAG 3 cut(s) 394, 1522, 3528
Bsh1236I CGCG 2 cut(s) 794, 1407
BshNI GGYRCC 5 cut(s) 998, 1367, 2340, 2531, 3402
BshTI ACCGGT 1 cut(s) 1067
BshVI ATCGAT 1 cut(s) 2193
BsiHKAI GWGCWC 6 cut(s) 248, 1022, 1202, 1458, 3107, 3321
BsiHKCI CYCGRG 7 cut(s) 222, 457, 494, 680, 2535, 3203, 3458
BslFI GGGAC 7 cut(s) 236, 749, 1525, 1691, 1975, 2350, 3011
BsmAI GTCTC 6 cut(s) 667, 677, 1044, 1125, 2240, 3052
BsmFI GGGAC 7 cut(s) 236, 749, 1525, 1691, 1975, 2350, 3011
BsmI GAATGC 2 cut(s) 2007, 2976
BsoBI CYCGRG 7 cut(s) 222, 457, 494, 680, 2535, 3203, 3458
Bsp1286I GDGCHC 9 cut(s) 248, 1001, 1022, 1202, 1458, 2343, 2536, 3107, 3321
Bsp1720I GCTNAGC 2 cut(s) 1100, 1433
Bsp19I CCATGG 2 cut(s) 1831, 3398
Bsp68I TCGCGA 1 cut(s) 1407
BspACI CCGC 6 cut(s) 576, 1437, 1769, 1859, 2727, 3406
BspDI ATCGAT 1 cut(s) 2193
BspFNI CGCG 2 cut(s) 794, 1407
BspHI TCATGA 2 cut(s) 1458, 3321
BspMAI CTGCAG 1 cut(s) 608
BspPI GGATC 7 cut(s) 596, 981, 1200, 1238, 1710, 2947, 3305
BspT107I GGYRCC 5 cut(s) 998, 1367, 2340, 2531, 3402
BsrDI GCAATG 3 cut(s) 1764, 2391, 3087
BsrFI RCCGGY 4 cut(s) 341, 569, 647, 1067
BsrI ACTGG 4 cut(s) 1219, 1369, 2239, 2958
BssAI RCCGGY 4 cut(s) 341, 569, 647, 1067
BssNAI GTATAC 1 cut(s) 2668
BssNI GRCGYC 4 cut(s) 111, 789, 3223, 3403
BssT1I CCWWGG 5 cut(s) 507, 1032, 1831, 2803, 3398
Bst1107I GTATAC 1 cut(s) 2668
Bst4CI ACNGT 9 cut(s) 715, 740, 1003, 1136, 1322, 1682, 2397, 2495, 2636
Bst6I CTCTTC 4 cut(s) 62, 85, 147, 811
BstACI GRCGYC 4 cut(s) 111, 789, 3223, 3403
BstAPI GCANNNNNTGC 1 cut(s) 1827
BstDSI CCRYGG 4 cut(s) 584, 714, 1831, 3398
BstF5I GGATG 4 cut(s) 1371, 2692, 3394, 3468
BstFNI CGCG 2 cut(s) 794, 1407
BstH2I RGCGCY 1 cut(s) 3406
BstHHI GCGC 5 cut(s) 796, 1028, 3380, 3405, 3433
BstMAI GTCTC 6 cut(s) 667, 677, 1044, 1125, 2240, 3052
BstNSI RCATGY 2 cut(s) 800, 3284
BstPAI GACNNNNGTC 1 cut(s) 2362
BstSFI CTRYAG 2 cut(s) 604, 3228
BstSLI GKGCMC 3 cut(s) 1001, 2343, 2536
BstUI CGCG 2 cut(s) 794, 1407
BstV1I GCAGC 7 cut(s) 387, 590, 618, 829, 1137, 2703, 3195
BstV2I GAAGAC 2 cut(s) 3226, 3546
BstX2I RGATCY 7 cut(s) 397, 973, 1192, 1243, 1702, 2599, 2939
BstXI CCANNNNNNTGG 1 cut(s) 2185
BstYI RGATCY 7 cut(s) 397, 973, 1192, 1243, 1702, 2599, 2939
BstZ17I GTATAC 1 cut(s) 2668
Bsu15I ATCGAT 1 cut(s) 2193
BsuI GTATCC 2 cut(s) 604, 2801
BsuTUI ATCGAT 1 cut(s) 2193
BtgI CCRYGG 4 cut(s) 584, 714, 1831, 3398
BtgZI GCGATG 1 cut(s) 3189
BtsCI GGATG 4 cut(s) 1371, 2692, 3394, 3468
BtsI GCAGTG 2 cut(s) 2383, 2748
BtsIMutI CAGTG 4 cut(s) 1212, 1362, 2383, 2748
BtuMI TCGCGA 1 cut(s) 1407
CaiI CAGNNNCTG 1 cut(s) 1320
CciI TCATGA 2 cut(s) 1458, 3321
CfoI GCGC 5 cut(s) 796, 1028, 3380, 3405, 3433
Cfr10I RCCGGY 4 cut(s) 341, 569, 647, 1067
Cfr9I CCCGGG 2 cut(s) 222, 3458
ClaI ATCGAT 1 cut(s) 2193
CseI GACGC 2 cut(s) 119, 2352
CsiI ACCWGGT 1 cut(s) 3121
Csp6I GTAC 9 cut(s) 1146, 1270, 1296, 1323, 1368, 1728, 2032, 2511, 2848
CspAI ACCGGT 1 cut(s) 1067
CspCI CAANNNNNGTGG 4 cut(s) 921, 956, 1561, 1596
CviQI GTAC 9 cut(s) 1146, 1270, 1296, 1323, 1368, 1728, 2032, 2511, 2848
DinI GGCGCC 1 cut(s) 3404
DrdI GACNNNNNNGTC 1 cut(s) 659
DseDI GACNNNNNNGTC 1 cut(s) 659
EaeI YGGCCR 2 cut(s) 516, 867
Eam1104I CTCTTC 4 cut(s) 62, 85, 147, 811
EarI CTCTTC 4 cut(s) 62, 85, 147, 811
Ecl136II GAGCTC 5 cut(s) 246, 1020, 1200, 1456, 3319
Eco130I CCWWGG 5 cut(s) 507, 1032, 1831, 2803, 3398
Eco147I AGGCCT 1 cut(s) 2283
Eco24I GRGCYC 5 cut(s) 248, 1022, 1202, 1458, 3321
Eco32I GATATC 1 cut(s) 1981
Eco47I GGWCC 6 cut(s) 541, 751, 857, 2107, 3331, 3455
Eco53kI GAGCTC 5 cut(s) 246, 1020, 1200, 1456, 3319
Eco57I CTGAAG 2 cut(s) 872, 1649
Eco88I CYCGRG 7 cut(s) 222, 457, 494, 680, 2535, 3203, 3458
EcoICRI GAGCTC 5 cut(s) 246, 1020, 1200, 1456, 3319
EcoO109I RGGNCCY 4 cut(s) 541, 2107, 3331, 3455
EcoRI GAATTC 2 cut(s) 2302, 2744
EcoRV GATATC 1 cut(s) 1981
EcoT14I CCWWGG 5 cut(s) 507, 1032, 1831, 2803, 3398
EcoT22I ATGCAT 1 cut(s) 1015
EcoT38I GRGCYC 5 cut(s) 248, 1022, 1202, 1458, 3321
EgeI GGCGCC 1 cut(s) 3404
EheI GGCGCC 1 cut(s) 3404
ErhI CCWWGG 5 cut(s) 507, 1032, 1831, 2803, 3398
FalI AAGNNNNNCTT 2 cut(s) 2817, 2849
FaqI GGGAC 7 cut(s) 236, 749, 1525, 1691, 1975, 2350, 3011
FauI CCCGC 4 cut(s) 583, 1430, 1852, 2720
FbaI TGATCA 1 cut(s) 1413
FblI GTMKAC 1 cut(s) 2667
Fnu4HI GCNGC 8 cut(s) 376, 604, 607, 818, 1151, 2692, 3209, 3406
FokI GGATG 4 cut(s) 1358, 2699, 3401, 3475
FriOI GRGCYC 5 cut(s) 248, 1022, 1202, 1458, 3321
Fsp4HI GCNGC 8 cut(s) 376, 604, 607, 818, 1151, 2692, 3209, 3406
FspBI CTAG 7 cut(s) 401, 623, 962, 1917, 2087, 2130, 3035
GlaI GCGC 5 cut(s) 795, 1027, 3379, 3404, 3432
GluI GCNGC 8 cut(s) 376, 604, 607, 818, 1151, 2692, 3209, 3406
GsaI CCCAGC 3 cut(s) 118, 939, 1676
GsuI CTGGAG 8 cut(s) 521, 823, 1259, 1290, 1496, 1871, 2094, 3120
HaeII RGCGCY 1 cut(s) 3406
HgaI GACGC 2 cut(s) 119, 2352
HhaI GCGC 5 cut(s) 796, 1028, 3380, 3405, 3433
Hin1I GRCGYC 4 cut(s) 111, 789, 3223, 3403
Hin6I GCGC 5 cut(s) 794, 1026, 3378, 3403, 3431
HinP1I GCGC 5 cut(s) 794, 1026, 3378, 3403, 3431
HindIII AAGCTT 3 cut(s) 1400, 1607, 2863
Hpy166II GTNNAC 4 cut(s) 261, 2491, 2668, 2842
Hpy8I GTNNAC 4 cut(s) 261, 2491, 2668, 2842
Hpy99I CGWCG 5 cut(s) 602, 791, 794, 1897, 3494
HpyAV CCTTC 6 cut(s) 167, 565, 1255, 2098, 2458, 2677
HpyCH4III ACNGT 9 cut(s) 715, 740, 1003, 1136, 1322, 1682, 2397, 2495, 2636
HpyCH4IV ACGT 7 cut(s) 521, 789, 824, 1793, 2481, 3015, 3223
HpySE526I ACGT 7 cut(s) 521, 789, 824, 1793, 2481, 3015, 3223
Hsp92I GRCGYC 4 cut(s) 111, 789, 3223, 3403
HspAI GCGC 5 cut(s) 794, 1026, 3378, 3403, 3431
KasI GGCGCC 1 cut(s) 3402
KpnI GGTACC 1 cut(s) 1371
KroI GCCGGC 1 cut(s) 569
KroNI GCCGGC 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 1413
LmnI GCTCC 9 cut(s) 177, 251, 967, 1223, 1264, 1453, 1669, 1835, 2075
Lsp1109I GCAGC 7 cut(s) 387, 590, 618, 829, 1137, 2703, 3195
LweI GCATC 4 cut(s) 1000, 2305, 2421, 2559
MabI ACCWGGT 1 cut(s) 3121
MaeI CTAG 7 cut(s) 401, 623, 962, 1917, 2087, 2130, 3035
MaeII ACGT 7 cut(s) 521, 789, 824, 1793, 2481, 3015, 3223
MaeIII GTNAC 4 cut(s) 1532, 1637, 3248, 3354
MfeI CAATTG 5 cut(s) 166, 1877, 2436, 2678, 2971
MflI RGATCY 7 cut(s) 397, 973, 1192, 1243, 1702, 2599, 2939
MhlI GDGCHC 9 cut(s) 248, 1001, 1022, 1202, 1458, 2343, 2536, 3107, 3321
Mly113I GGCGCC 1 cut(s) 3403
MlyI GAGTC 7 cut(s) 506, 522, 679, 965, 1046, 3054, 3461
MmeI TCCRAC 5 cut(s) 621, 726, 1077, 1680, 1963
Mph1103I ATGCAT 1 cut(s) 1015
MroNI GCCGGC 1 cut(s) 569
MroXI GAANNNNTTC 1 cut(s) 1208
MseI TTAA 5 cut(s) 474, 1950, 2546, 3290, 3365
MslI CAYNNNNRTG 4 cut(s) 1769, 1919, 2183, 3096
MspA1I CMGCKG 4 cut(s) 820, 1257, 2727, 3239
MunI CAATTG 5 cut(s) 166, 1877, 2436, 2678, 2971
Mva1269I GAATGC 2 cut(s) 2007, 2976
MvnI CGCG 2 cut(s) 794, 1407
NaeI GCCGGC 1 cut(s) 571
NarI GGCGCC 1 cut(s) 3403
NciI CCSGG 7 cut(s) 223, 224, 1510, 1667, 3459, 3460, 3552
NcoI CCATGG 2 cut(s) 1831, 3398
NgoMIV GCCGGC 1 cut(s) 569
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 1 cut(s) 3248
NruI TCGCGA 1 cut(s) 1407
NsiI ATGCAT 1 cut(s) 1015
NspI RCATGY 2 cut(s) 800, 3284
OliI CACNNNNGTG 1 cut(s) 1919
PaeI GCATGC 1 cut(s) 800
PaeR7I CTCGAG 4 cut(s) 457, 494, 680, 3203
PagI TCATGA 2 cut(s) 1458, 3321
PceI AGGCCT 1 cut(s) 2283
PcsI WCGNNNNNNNCGW 3 cut(s) 606, 687, 3021
PctI GAATGC 2 cut(s) 2007, 2976
PdiI GCCGGC 1 cut(s) 571
PdmI GAANNNNTTC 1 cut(s) 1208
PfoI TCCNGGA 1 cut(s) 1488
PinAI ACCGGT 1 cut(s) 1067
PkrI GCNGC 8 cut(s) 377, 605, 608, 819, 1152, 2693, 3210, 3407
PleI GAGTC 7 cut(s) 506, 522, 679, 964, 1045, 3053, 3461
PluTI GGCGCC 1 cut(s) 3406
PpsI GAGTC 7 cut(s) 506, 522, 679, 964, 1045, 3053, 3461
PpuMI RGGWCCY 4 cut(s) 541, 2107, 3331, 3455
PshAI GACNNNNGTC 1 cut(s) 2362
Psp124BI GAGCTC 5 cut(s) 248, 1022, 1202, 1458, 3321
Psp5II RGGWCCY 4 cut(s) 541, 2107, 3331, 3455
PspFI CCCAGC 3 cut(s) 114, 935, 1672
PspPPI RGGWCCY 4 cut(s) 541, 2107, 3331, 3455
PspXI VCTCGAGB 3 cut(s) 457, 494, 3203
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PstI CTGCAG 1 cut(s) 608
PstNI CAGNNNCTG 1 cut(s) 1320
PsuI RGATCY 7 cut(s) 397, 973, 1192, 1243, 1702, 2599, 2939
PvuII CAGCTG 3 cut(s) 820, 1257, 3239
RruI TCGCGA 1 cut(s) 1407
RsaI GTAC 9 cut(s) 1147, 1271, 1297, 1324, 1369, 1729, 2033, 2512, 2849
RsaNI GTAC 9 cut(s) 1146, 1270, 1296, 1323, 1368, 1728, 2032, 2511, 2848
RseI CAYNNNNRTG 4 cut(s) 1769, 1919, 2183, 3096
SacI GAGCTC 5 cut(s) 248, 1022, 1202, 1458, 3321
SaqAI TTAA 5 cut(s) 474, 1950, 2546, 3290, 3365
SatI GCNGC 8 cut(s) 376, 604, 607, 818, 1151, 2692, 3209, 3406
SchI GAGTC 7 cut(s) 506, 522, 679, 965, 1046, 3054, 3461
SduI GDGCHC 9 cut(s) 248, 1001, 1022, 1202, 1458, 2343, 2536, 3107, 3321
SexAI ACCWGGT 1 cut(s) 3121
SfaNI GCATC 4 cut(s) 1000, 2305, 2421, 2559
SfcI CTRYAG 2 cut(s) 604, 3228
SfoI GGCGCC 1 cut(s) 3404
Sfr274I CTCGAG 4 cut(s) 457, 494, 680, 3203
SgrAI CRCCGGYG 2 cut(s) 647, 1067
SinI GGWCC 6 cut(s) 541, 751, 857, 2107, 3331, 3455
SlaI CTCGAG 4 cut(s) 457, 494, 680, 3203
SmaI CCCGGG 2 cut(s) 224, 3460
SmiMI CAYNNNNRTG 4 cut(s) 1769, 1919, 2183, 3096
SmlI CTYRAG 7 cut(s) 457, 494, 629, 680, 2606, 3203, 3341
SmoI CTYRAG 7 cut(s) 457, 494, 629, 680, 2606, 3203, 3341
SpeI ACTAGT 1 cut(s) 622
SphI GCATGC 1 cut(s) 800
SseBI AGGCCT 1 cut(s) 2283
SsiI CCGC 6 cut(s) 576, 1437, 1769, 1859, 2727, 3406
SspDI GGCGCC 1 cut(s) 3402
SspMI CTAG 7 cut(s) 401, 623, 962, 1917, 2087, 2130, 3035
SstI GAGCTC 5 cut(s) 248, 1022, 1202, 1458, 3321
StuI AGGCCT 1 cut(s) 2283
StyI CCWWGG 5 cut(s) 507, 1032, 1831, 2803, 3398
TaaI ACNGT 9 cut(s) 715, 740, 1003, 1136, 1322, 1682, 2397, 2495, 2636
TaiI ACGT 7 cut(s) 524, 792, 827, 1796, 2484, 3018, 3226
TatI WGTACW 3 cut(s) 1322, 2031, 2847
TauI GCSGC 1 cut(s) 3408
Tru1I TTAA 5 cut(s) 474, 1950, 2546, 3290, 3365
Tru9I TTAA 5 cut(s) 474, 1950, 2546, 3290, 3365
TscAI CASTG 4 cut(s) 1219, 1369, 2383, 2755
TseFI GTSAC 1 cut(s) 3248
TseI GCWGC 7 cut(s) 375, 603, 606, 817, 1150, 2691, 3208
Tsp45I GTSAC 1 cut(s) 3248
TspDTI ATGAA 9 cut(s) 17, 84, 1164, 1475, 2285, 2867, 2981, 3017, 3338
TspGWI ACGGA 2 cut(s) 238, 714
TspMI CCCGGG 2 cut(s) 222, 3458
TspRI CASTG 4 cut(s) 1219, 1369, 2383, 2755
VpaK11BI GGWCC 6 cut(s) 541, 751, 857, 2107, 3331, 3455
XapI RAATTY 6 cut(s) 776, 1443, 1774, 1843, 2302, 2744
XbaI TCTAGA 1 cut(s) 3034
XceI RCATGY 2 cut(s) 800, 3284
XcmI CCANNNNNNNNNTGG 1 cut(s) 2684
XhoI CTCGAG 4 cut(s) 457, 494, 680, 3203
XmaI CCCGGG 2 cut(s) 222, 3458
XmiI GTMKAC 1 cut(s) 2667
XmnI GAANNNNTTC 1 cut(s) 1208
XspI CTAG 7 cut(s) 401, 623, 962, 1917, 2087, 2130, 3035
ZraI GACGTC 2 cut(s) 790, 3224
Zsp2I ATGCAT 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.