AT4G29400

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006686
Physical Location & Seq
Forward (+)
14466267 .. 14468267
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29400.1

Sequence Viewer

Length: 909 bp
ATGCTAAGGTTTCAATGTTTTCTTCACCTTCTTCAGCCTTCTATGGCGAAGAACAACAGCAACCACTCGAATATTTGTTTACACAATTTGGCGCCGTTTCGGGTCACCGACACGATTCCTGTACAGAAGATCCAGGAGTTACGTGCGAATCGGTCGAGGGAGAGTGGAAAGTTGCGAGTAAATGGAATAGGTAGAGAAATGGAAGAAGGTGTGGAAGAATTCGAAGAAGTAGATGATGGAGATGATGATGAGGTAGAAGATGAGTTCTCGGCGAAGAAGAGAGGCGTTTACAGAGCGAAGAAAGAGAAGATTGATTACGATAAAGACCCTGAGTTTGCGGATATTCTTGGAGATTGTTTAGATAACCCAGATAAAGCTCAAAAGAAGATGGAAGAGAGATTGAGGAAGAAGAGGAACAAAATTCTTCATACTAAGACTGGTTCTGCAACTTCGATGCCAGTGACGTTTAACAAATTTGAGTATTCGAATTCATACATGTGGTTGGAGTTTTACAACACACCGCTGGACAAAGACATAGCCTTGATCTCTGATACAATCCGGTCCTGGCATATCCTTGGACGACTTGGTGGATACAACTCCATGAATATGCAATTATCACAAGCACCACTGGATAAGAGGCCAAACTATGATGCCATACTTGGAGCTAATGTCGAGCCCACCACGTTTTATAACATCGGGGATCTTGAGGTTCAAGACAATGTCGCTCGAATATGGCTCGATATTGGGACCTCGGAGCCGTTGATTCTCGATGTTCTGATAAACGCATTGACACAAATCAGCTCAGATTATGTCGGGATAAAGAAAGTTGTGTTTGGTGGATCTGAGTTCGAGAGCTGGAAGGAGAATATGACATCCGAGGAATCTGGTTTCAGAGTCCACAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

302

Amino Acids

34.64

Weight (kDa)

5.25

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 152 - 293 8.4e-53 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AccB1I GGYRCC 1 cut(s) 91
AciI CCGC 2 cut(s) 338, 521
AclWI GGATC 3 cut(s) 124, 708, 847
AcsI RAATTY 4 cut(s) 218, 420, 473, 487
AcuI CTGAAG 1 cut(s) 17
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 123
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 14, 713
AjnI CCWGG 2 cut(s) 132, 563
AluBI AGCT 4 cut(s) 377, 665, 801, 855
AluI AGCT 4 cut(s) 377, 665, 801, 855
AlwI GGATC 3 cut(s) 124, 708, 847
AoxI GGCC 1 cut(s) 638
ApoI RAATTY 4 cut(s) 218, 420, 473, 487
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 561, 747
AsuHPI GGTGA 2 cut(s) 17, 97
AsuII TTCGAA 2 cut(s) 222, 485
AvaII GGWCC 2 cut(s) 561, 747
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 678
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BccI CCATC 2 cut(s) 230, 382
BceAI ACGGC 2 cut(s) 79, 742
BciT130I CCWGG 2 cut(s) 134, 565
BciVI GTATCC 1 cut(s) 584
BfoI RGCGCY 1 cut(s) 95
BfuI GTATCC 1 cut(s) 584
Bme1390I CCNGG 2 cut(s) 134, 565
Bme18I GGWCC 2 cut(s) 561, 747
BmgT120I GGNCC 2 cut(s) 561, 747
BmiI GGNNCC 3 cut(s) 93, 748, 756
BmrFI CCNGG 2 cut(s) 134, 565
BmsI GCATC 2 cut(s) 444, 640
Bpu10I CCTNAGC 1 cut(s) 5
Bpu14I TTCGAA 2 cut(s) 222, 485
BpuEI CTTGAG 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 574, 750, 876
BsaWI WCCGGW 1 cut(s) 558
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bse1I ACTGG 3 cut(s) 442, 458, 633
BseBI CCWGG 2 cut(s) 134, 565
BseDI CCNNGG 3 cut(s) 574, 750, 876
BseGI GGATG 1 cut(s) 872
BseMII CTCAG 3 cut(s) 321, 816, 834
BseNI ACTGG 3 cut(s) 442, 458, 633
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 640
BshNI GGYRCC 1 cut(s) 91
BsiEI CGRYCG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 559
BslFI GGGAC 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 760
BsnI GGCC 1 cut(s) 640
Bsp119I TTCGAA 2 cut(s) 222, 485
Bsp1286I GDGCHC 1 cut(s) 678
Bsp1407I TGTACA 1 cut(s) 121
Bsp143I GATC 4 cut(s) 129, 543, 700, 839
BspACI CCGC 2 cut(s) 338, 521
BspANI GGCC 1 cut(s) 640
BspCNI CTCAG 3 cut(s) 322, 815, 835
BspLI GGNNCC 3 cut(s) 93, 748, 756
BspPI GGATC 3 cut(s) 124, 708, 847
BspT104I TTCGAA 2 cut(s) 222, 485
BspT107I GGYRCC 1 cut(s) 91
BsrGI TGTACA 1 cut(s) 121
BsrI ACTGG 3 cut(s) 442, 458, 633
BssECI CCNNGG 3 cut(s) 574, 750, 876
BssMI GATC 4 cut(s) 129, 543, 700, 839
BssNI GRCGYC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 134, 565
Bst6I CTCTTC 3 cut(s) 272, 387, 404
BstACI GRCGYC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 121
BstBAI YACGTR 1 cut(s) 143
BstBI TTCGAA 2 cut(s) 222, 485
BstDEI CTNAG 5 cut(s) 5, 330, 432, 802, 843
BstEII GGTNACC 1 cut(s) 103
BstF5I GGATG 1 cut(s) 872
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 4 cut(s) 132, 546, 703, 842
BstMBI GATC 4 cut(s) 129, 543, 700, 839
BstMCI CGRYCG 1 cut(s) 155
BstNI CCWGG 2 cut(s) 134, 565
BstNSI RCATGY 1 cut(s) 499
BstPI GGTNACC 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 132, 563
BstX2I RGATCY 3 cut(s) 129, 700, 839
BstYI RGATCY 3 cut(s) 129, 700, 839
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 1 cut(s) 640
BtsCI GGATG 1 cut(s) 872
BtsIMutI CAGTG 2 cut(s) 465, 626
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 561, 747
Csp6I GTAC 1 cut(s) 122
CviAII CATG 2 cut(s) 496, 601
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 5 cut(s) 5, 330, 432, 802, 843
DinI GGCGCC 1 cut(s) 93
DpnI GATC 4 cut(s) 131, 545, 702, 841
DpnII GATC 4 cut(s) 129, 543, 700, 839
Eam1104I CTCTTC 3 cut(s) 272, 387, 404
EarI CTCTTC 3 cut(s) 272, 387, 404
Eco130I CCWWGG 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 678
Eco47I GGWCC 2 cut(s) 561, 747
Eco57I CTGAAG 1 cut(s) 17
Eco91I GGTNACC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 747
EcoO65I GGTNACC 1 cut(s) 103
EcoRI GAATTC 2 cut(s) 218, 487
EcoRII CCWGG 2 cut(s) 132, 563
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 678
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 2 cut(s) 499, 604
FaqI GGGAC 1 cut(s) 760
FatI CATG 2 cut(s) 495, 600
FokI GGATG 1 cut(s) 859
FriOI GRGCYC 1 cut(s) 678
GlaI GCGC 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 1 cut(s) 640
HapII CCGG 1 cut(s) 559
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 2 cut(s) 499, 604
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 5 cut(s) 115, 148, 763, 881, 894
HpaII CCGG 1 cut(s) 559
HphI GGTGA 2 cut(s) 17, 97
Hpy166II GTNNAC 3 cut(s) 80, 289, 898
Hpy188I TCNGA 7 cut(s) 550, 754, 777, 805, 844, 877, 893
Hpy188III TCNNGA 5 cut(s) 704, 713, 767, 814, 850
Hpy8I GTNNAC 3 cut(s) 80, 289, 898
HpyAV CCTTC 4 cut(s) 38, 48, 200, 853
HpyCH4IV ACGT 3 cut(s) 142, 464, 683
HpyCH4V TGCA 2 cut(s) 446, 610
HpyF3I CTNAG 5 cut(s) 5, 330, 432, 802, 843
HpySE526I ACGT 3 cut(s) 142, 464, 683
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 2 cut(s) 499, 604
HspAI GCGC 1 cut(s) 92
KasI GGCGCC 1 cut(s) 91
Kzo9I GATC 4 cut(s) 129, 543, 700, 839
LmnI GCTCC 2 cut(s) 662, 754
LweI GCATC 2 cut(s) 444, 640
MaeII ACGT 3 cut(s) 142, 464, 683
MaeIII GTNAC 3 cut(s) 103, 138, 460
MalI GATC 4 cut(s) 131, 545, 702, 841
MboI GATC 4 cut(s) 129, 543, 700, 839
MflI RGATCY 3 cut(s) 129, 700, 839
MhlI GDGCHC 1 cut(s) 678
MluCI AATT 6 cut(s) 85, 218, 420, 473, 487, 611
Mly113I GGCGCC 1 cut(s) 92
MlyI GAGTC 1 cut(s) 903
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 9 cut(s) 150, 244, 275, 396, 405, 630, 700, 760, 871
MseI TTAA 2 cut(s) 468, 907
MslI CAYNNNNRTG 2 cut(s) 496, 605
MspA1I CMGCKG 1 cut(s) 523
MspI CCGG 1 cut(s) 559
MspR9I CCNGG 2 cut(s) 134, 565
MvaI CCWGG 2 cut(s) 134, 565
NarI GGCGCC 1 cut(s) 92
NdeII GATC 4 cut(s) 129, 543, 700, 839
NlaIII CATG 2 cut(s) 499, 604
NlaIV GGNNCC 3 cut(s) 93, 748, 756
NmeAIII GCCGAG 1 cut(s) 248
NmuCI GTSAC 2 cut(s) 103, 460
NspI RCATGY 1 cut(s) 499
NspV TTCGAA 2 cut(s) 222, 485
PciI ACATGT 1 cut(s) 495
PfeI GAWTC 4 cut(s) 115, 148, 763, 881
PflFI GACNNNGTC 1 cut(s) 719
PfoI TCCNGGA 1 cut(s) 132
PleI GAGTC 1 cut(s) 902
PluTI GGCGCC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 902
Ppu21I YACGTR 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 747
PscI ACATGT 1 cut(s) 495
PsiI TTATAA 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 132, 563
PspEI GGTNACC 1 cut(s) 103
PspGI CCWGG 2 cut(s) 132, 563
PspN4I GGNNCC 3 cut(s) 93, 748, 756
PspPI GGNCC 2 cut(s) 561, 747
PspPPI RGGWCCY 1 cut(s) 747
PsuI RGATCY 3 cut(s) 129, 700, 839
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
RseI CAYNNNNRTG 2 cut(s) 496, 605
SaqAI TTAA 2 cut(s) 468, 907
Sau3AI GATC 4 cut(s) 129, 543, 700, 839
Sau96I GGNCC 2 cut(s) 561, 747
SchI GAGTC 1 cut(s) 903
ScrFI CCNGG 2 cut(s) 134, 565
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 2 cut(s) 444, 640
SfoI GGCGCC 1 cut(s) 93
SfuI TTCGAA 2 cut(s) 222, 485
SinI GGWCC 2 cut(s) 561, 747
SmiMI CAYNNNNRTG 2 cut(s) 496, 605
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
Sse9I AATT 6 cut(s) 85, 218, 420, 473, 487, 611
SsiI CCGC 2 cut(s) 338, 521
SspDI GGCGCC 1 cut(s) 91
SspI AATATT 1 cut(s) 73
StyD4I CCNGG 2 cut(s) 132, 563
StyI CCWWGG 1 cut(s) 574
TaiI ACGT 3 cut(s) 145, 467, 686
TaqII GACCGA 1 cut(s) 141
TasI AATT 6 cut(s) 85, 218, 420, 473, 487, 611
TatI WGTACW 1 cut(s) 121
TfiI GAWTC 4 cut(s) 115, 148, 763, 881
Tru1I TTAA 2 cut(s) 468, 907
Tru9I TTAA 2 cut(s) 468, 907
TscAI CASTG 2 cut(s) 465, 633
TseFI GTSAC 2 cut(s) 103, 460
Tsp45I GTSAC 2 cut(s) 103, 460
TspDTI ATGAA 3 cut(s) 416, 480, 617
TspRI CASTG 2 cut(s) 465, 633
Tth111I GACNNNGTC 1 cut(s) 719
VpaK11BI GGWCC 2 cut(s) 561, 747
XapI RAATTY 4 cut(s) 218, 420, 473, 487
XceI RCATGY 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.