Rroxscaffold_2G00148960

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006686
Physical Location & Seq
Reverse (-)
86590311 .. 86592924
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148960.1

Sequence Viewer

Length: 888 bp
ATGGCTGTCTTACACAGCTGCGCGCCTTTTCTGCAAAGTCCGAGCATTCCCTTATCCTTGTTTTCCCGCCAAAAACACTGCTCGCCACCATTTTGGGCCGTCGGAGGTCCATTTCGGAGCTGTTATGGTCAGCCCAAGAAGAGTGTATTGGTGGCTAAAGCGGGTCGGGGTCTAAAGTTGGGGACCCAGAAAGAGAGTGGAGGAGAAGATGGAGGCTTTGGTGATGAAGAAGGTGAAGGTGAAGATGGGTTTTCGAGTGGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTTGATCGAGACCCTGAGTTTGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAAAAGGAACAAGATACTGCATACAAAATCTGGGTCAGGGGAAGCCATGAGAGTGGTATTCAATGACTTTGGTTTCTCAAATTCATACATATGGTTCGAGTTCTACAACATTCCATTGGAACAAGATGTTTCCTTAATCTCAGATACTATTCGATCGTGGCACATTATTGGACGCCTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAATCCCCTTTGGACAAACGACCAAGTTATGATCCCATTCAGGGAGCAAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGATTCAAGACAACTTGGGACGTGTTTGGGTGGATATTGGTACTAAAGAGCCACTGATACTGGATGTTCTGATAAATGCATTGACTCAGATAAGCTCCGACTTTGTTGGAATCAAGCAAGTGGTGTTTGGTGGAACTGAATTTGAACACTGGAAGGAGAACATGACATCAGAGGAAGTTGGTTATACTGTTCACAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

295

Amino Acids

32.66

Weight (kDa)

5.48

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 145 - 286 8.6e-52 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 67, 161
AclWI GGATC 1 cut(s) 623
AcsI RAATTY 2 cut(s) 466, 824
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 2 cut(s) 352, 638
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 3 cut(s) 448, 692, 830
AjiI CACGTC 1 cut(s) 707
AjnI CCWGG 1 cut(s) 561
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 3 cut(s) 18, 120, 780
AluI AGCT 3 cut(s) 18, 120, 780
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 1 cut(s) 623
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 466, 824
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 404, 436
AspLEI GCGC 2 cut(s) 23, 25
AspS9I GGNCC 4 cut(s) 96, 107, 183, 360
AsuHPI GGTGA 3 cut(s) 233, 245, 251
AvaII GGWCC 3 cut(s) 107, 183, 360
BbvI GCAGC 1 cut(s) 5
BccI CCATC 3 cut(s) 203, 239, 361
BceAI ACGGC 1 cut(s) 83
BciT130I CCWGG 1 cut(s) 563
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 1 cut(s) 357
BglII AGATCT 1 cut(s) 679
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 563
Bme18I GGWCC 3 cut(s) 107, 183, 360
BmgBI CACGTC 1 cut(s) 707
BmgT120I GGNCC 4 cut(s) 96, 107, 183, 360
BmiI GGNNCC 2 cut(s) 184, 185
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 704
BsaHI GRCGYC 1 cut(s) 559
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 562
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 633, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 638
Bse1I ACTGG 2 cut(s) 750, 839
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 1 cut(s) 562
BseGI GGATG 2 cut(s) 575, 754
BseLI CCNNNNNNNGG 2 cut(s) 352, 638
BseMII CTCAG 3 cut(s) 300, 540, 785
BseNI ACTGG 2 cut(s) 750, 839
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 216, 294, 386
BseXI GCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 542
BslFI GGGAC 2 cut(s) 196, 717
BslI CCNNNNNNNGG 2 cut(s) 352, 638
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 2 cut(s) 196, 717
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 1 cut(s) 297
Bsp143I GATC 4 cut(s) 298, 539, 628, 679
BspACI CCGC 2 cut(s) 67, 161
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 3 cut(s) 301, 539, 784
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 184, 185
BspPI GGATC 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 297
BsrI ACTGG 2 cut(s) 750, 839
BssECI CCNNGG 1 cut(s) 562
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 4 cut(s) 298, 539, 628, 679
BssNI GRCGYC 1 cut(s) 559
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 23, 83
BstDEI CTNAG 3 cut(s) 309, 526, 771
BstF5I GGATG 2 cut(s) 575, 754
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 25
BstKTI GATC 4 cut(s) 301, 542, 631, 682
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 298, 539, 628, 679
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 561
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 2 cut(s) 93, 439
BstYI RGATCY 1 cut(s) 679
BsuRI GGCC 1 cut(s) 98
BtrI CACGTC 1 cut(s) 707
BtsCI GGATG 2 cut(s) 575, 754
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 737, 832
Cac8I GCNNGC 2 cut(s) 23, 83
CfoI GCGC 2 cut(s) 23, 25
Cfr13I GGNCC 4 cut(s) 96, 107, 183, 360
CseI GACGC 1 cut(s) 567
Csp6I GTAC 1 cut(s) 726
CviAII CATG 3 cut(s) 433, 580, 847
CviQI GTAC 1 cut(s) 726
DdeI CTNAG 3 cut(s) 309, 526, 771
DpnI GATC 4 cut(s) 300, 541, 630, 681
DpnII GATC 4 cut(s) 298, 539, 628, 679
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 297
Eco47I GGWCC 3 cut(s) 107, 183, 360
EcoO109I RGGNCCY 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 766
FaeI CATG 3 cut(s) 436, 583, 850
FaqI GGGAC 2 cut(s) 196, 717
FatI CATG 3 cut(s) 432, 579, 846
FauI CCCGC 2 cut(s) 74, 154
FauNDI CATATG 1 cut(s) 476
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 2 cut(s) 582, 761
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 1 cut(s) 357
GlaI GCGC 2 cut(s) 22, 24
GluI GCNGC 1 cut(s) 19
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 567
HhaI GCGC 2 cut(s) 23, 25
Hin1I GRCGYC 1 cut(s) 559
Hin1II CATG 3 cut(s) 436, 583, 850
Hin6I GCGC 2 cut(s) 21, 23
HinP1I GCGC 2 cut(s) 21, 23
HinfI GANTC 3 cut(s) 688, 769, 795
HphI GGTGA 3 cut(s) 233, 245, 251
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 8 cut(s) 42, 104, 117, 529, 756, 774, 784, 856
Hpy188III TCNNGA 4 cut(s) 302, 338, 683, 692
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 5 cut(s) 224, 230, 278, 283, 832
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 5 cut(s) 34, 406, 573, 589, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 3 cut(s) 309, 526, 771
HpySE526I ACGT 1 cut(s) 706
Hsp92I GRCGYC 1 cut(s) 559
Hsp92II CATG 3 cut(s) 436, 583, 850
HspAI GCGC 2 cut(s) 21, 23
KflI GGGWCCC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 298, 539, 628, 679
LmnI GCTCC 3 cut(s) 117, 641, 785
LpnPI CCDG 9 cut(s) 200, 321, 402, 408, 548, 575, 623, 731, 820
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 1 cut(s) 560
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 594, 649
MalI GATC 4 cut(s) 300, 541, 630, 681
MboI GATC 4 cut(s) 298, 539, 628, 679
MboII GAAGA 5 cut(s) 151, 218, 239, 254, 397
MfeI CAATTG 1 cut(s) 590
MflI RGATCY 1 cut(s) 679
MluCI AATT 4 cut(s) 466, 574, 590, 824
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 3 cut(s) 82, 772, 807
MnlI CCTC 8 cut(s) 98, 194, 206, 272, 364, 369, 375, 850
Mph1103I ATGCAT 1 cut(s) 766
MseI TTAA 1 cut(s) 521
MslI CAYNNNNRTG 3 cut(s) 437, 475, 584
MspA1I CMGCKG 1 cut(s) 18
MspR9I CCNGG 1 cut(s) 563
MunI CAATTG 1 cut(s) 590
Mva1269I GAATGC 1 cut(s) 45
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeI CATATG 1 cut(s) 476
NdeII GATC 4 cut(s) 298, 539, 628, 679
NlaIII CATG 3 cut(s) 436, 583, 850
NlaIV GGNNCC 2 cut(s) 184, 185
NmuCI GTSAC 2 cut(s) 594, 649
NsiI ATGCAT 1 cut(s) 766
PauI GCGCGC 1 cut(s) 21
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 688, 795
PkrI GCNGC 1 cut(s) 20
Ple19I CGATCG 1 cut(s) 542
PleI GAGTC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 763
PpuMI RGGWCCY 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 2 cut(s) 184, 185
PspPI GGNCC 4 cut(s) 96, 107, 183, 360
PspPPI RGGWCCY 1 cut(s) 183
PsuI RGATCY 1 cut(s) 679
PteI GCGCGC 1 cut(s) 21
PvuI CGATCG 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
RseI CAYNNNNRTG 3 cut(s) 437, 475, 584
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 4 cut(s) 298, 539, 628, 679
Sau96I GGNCC 4 cut(s) 96, 107, 183, 360
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 1 cut(s) 563
SetI ASST 9 cut(s) 20, 109, 122, 235, 241, 362, 380, 709, 782
SfaNI GCATC 1 cut(s) 560
SinI GGWCC 3 cut(s) 107, 183, 360
SmiMI CAYNNNNRTG 3 cut(s) 437, 475, 584
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 4 cut(s) 466, 574, 590, 824
SsiI CCGC 2 cut(s) 67, 161
SspI AATATT 1 cut(s) 673
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 561
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 709
TaqI TCGA 4 cut(s) 254, 301, 483, 538
TasI AATT 4 cut(s) 466, 574, 590, 824
TfiI GAWTC 2 cut(s) 688, 795
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 3 cut(s) 83, 744, 839
TseFI GTSAC 2 cut(s) 594, 649
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 2 cut(s) 594, 649
TspDTI ATGAA 3 cut(s) 240, 459, 596
TspRI CASTG 3 cut(s) 83, 744, 839
VpaK11BI GGWCC 3 cut(s) 107, 183, 360
XapI RAATTY 2 cut(s) 466, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XspI CTAG 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.