Rroxscaffold_2G00147660

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006686
Physical Location & Seq
Forward (+)
85385637 .. 85388652
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147660.1

Sequence Viewer

Length: 840 bp
ATGGCTGTCTTACACAGCTGCGCGCCTTTTCTGCAAACTCCGAGCGTTTCCTTATCCTTATTTTCCCGCCAGAAACACTGCTTGCCGCCGTTTTGGGCCATCGGAGGTCCATTTCGGAGCTGTTATGGTCAGCCCAAGACGAGTGTATTGGTTGCTAAAGCGGGTCGGGGTCAAAAGTTGAAGTCCCAGGAAAAGAGTGGAGGAGAAGATGGAGGCTTTGGTGATGAAGAGGGTGAAGGTGAAGATGGGTTTTCGAGTGGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTCGATCGAGACCCTGAGTATGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAACAGGAACAAGATATTGCTTACAAAATCGGGGTCAGGGGAGCCCATGAGAGTGGCTATTCGATCGTGGCACATTATTGGAAGCTTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAATCCCCTTTGGATAAACGGCCAAGTTATGATCCCATTCAGGGAGCAAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGACTCAAGTAAACTTGGGACGTGTTTGGGTGGATATTGGTACTAAAGAGCCACTGATACTGGATGTTCTGATAAATGCATTGACTCAGATAAGCTCCGAGTATGCTCTCTTTGTTGGAATCAAGCAAGTGGTGTTTGGTGGAACTGAATTTGAACACTGGAAGGAGAAAATGACATCGATCAGAGGAAGTTGGTTTTACTGTTCACAAGATTTAGCCAATTTGCTGCGCACATCTGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

279

Amino Acids

30.68

Weight (kDa)

6.15

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 148 - 257 1.2e-35 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 824
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 67, 86, 161
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 1 cut(s) 527
AcsI RAATTY 1 cut(s) 743
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 2 cut(s) 352, 548
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 2 cut(s) 181, 749
AjiI CACGTC 1 cut(s) 617
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 4 cut(s) 18, 120, 471, 690
AluI AGCT 4 cut(s) 18, 120, 471, 690
Alw26I GTCTC 2 cut(s) 297, 590
AlwI GGATC 1 cut(s) 533
AoxI GGCC 2 cut(s) 96, 527
ApeKI GCWGC 2 cut(s) 18, 820
ApoI RAATTY 1 cut(s) 743
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 3 cut(s) 23, 25, 825
AspS9I GGNCC 3 cut(s) 96, 107, 360
AsuHPI GGTGA 3 cut(s) 233, 245, 251
AvaII GGWCC 2 cut(s) 107, 360
BanII GRGCYC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 5, 807
BccI CCATC 4 cut(s) 107, 203, 239, 361
BceAI ACGGC 2 cut(s) 73, 542
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 297, 590
BfaI CTAG 1 cut(s) 357
BglII AGATCT 1 cut(s) 589
BisI GCNGC 3 cut(s) 19, 86, 821
BlsI GCNGC 3 cut(s) 20, 87, 822
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 2 cut(s) 107, 360
BmgBI CACGTC 1 cut(s) 617
BmgT120I GGNCC 3 cut(s) 96, 107, 360
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 188
BmsI GCATC 1 cut(s) 470
BpuEI CTTGAG 2 cut(s) 585, 614
Bsa29I ATCGAT 1 cut(s) 773
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 186
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 543, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 548
Bse1I ACTGG 2 cut(s) 660, 758
BseBI CCWGG 1 cut(s) 188
BseCI ATCGAT 1 cut(s) 773
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 485, 664
BseLI CCNNNNNNNGG 2 cut(s) 352, 548
BseMII CTCAG 2 cut(s) 300, 695
BseNI ACTGG 2 cut(s) 660, 758
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 216, 294, 386
BseXI GCAGC 2 cut(s) 5, 807
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 2 cut(s) 301, 452
BshFI GGCC 2 cut(s) 98, 529
BshVI ATCGAT 1 cut(s) 773
BsiEI CGRYCG 2 cut(s) 301, 452
BslFI GGGAC 2 cut(s) 169, 627
BslI CCNNNNNNNGG 2 cut(s) 352, 548
BsmAI GTCTC 2 cut(s) 297, 590
BsmFI GGGAC 2 cut(s) 169, 627
BsnI GGCC 2 cut(s) 98, 529
Bso31I GGTCTC 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 5 cut(s) 298, 449, 538, 589, 774
BspACI CCGC 3 cut(s) 67, 86, 161
BspANI GGCC 2 cut(s) 98, 529
BspCNI CTCAG 2 cut(s) 301, 694
BspDI ATCGAT 1 cut(s) 773
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 429
BspPI GGATC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 297
BsrI ACTGG 2 cut(s) 660, 758
BssECI CCNNGG 1 cut(s) 186
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 5 cut(s) 298, 449, 538, 589, 774
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 23, 83
BstDEI CTNAG 2 cut(s) 309, 681
BstF5I GGATG 2 cut(s) 485, 664
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 3 cut(s) 23, 25, 825
BstKTI GATC 5 cut(s) 301, 452, 541, 592, 777
BstMAI GTCTC 2 cut(s) 297, 590
BstMBI GATC 5 cut(s) 298, 449, 538, 589, 774
BstMCI CGRYCG 2 cut(s) 301, 452
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 2 cut(s) 5, 807
BstX2I RGATCY 1 cut(s) 589
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 589
Bsu15I ATCGAT 1 cut(s) 773
BsuRI GGCC 2 cut(s) 98, 529
BsuTUI ATCGAT 1 cut(s) 773
BtrI CACGTC 1 cut(s) 617
BtsCI GGATG 2 cut(s) 485, 664
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 647, 751
Cac8I GCNNGC 2 cut(s) 23, 83
CfoI GCGC 3 cut(s) 23, 25, 825
Cfr13I GGNCC 3 cut(s) 96, 107, 360
ClaI ATCGAT 1 cut(s) 773
Csp6I GTAC 1 cut(s) 636
CviAII CATG 2 cut(s) 433, 490
CviQI GTAC 1 cut(s) 636
DdeI CTNAG 2 cut(s) 309, 681
DpnI GATC 5 cut(s) 300, 451, 540, 591, 776
DpnII GATC 5 cut(s) 298, 449, 538, 589, 774
EaeI YGGCCR 1 cut(s) 527
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 297
Eco47I GGWCC 2 cut(s) 107, 360
EcoRII CCWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 2 cut(s) 436, 493
FaiI YATR 7 cut(s) 126, 315, 434, 491, 497, 537, 699
FaqI GGGAC 2 cut(s) 169, 627
FatI CATG 2 cut(s) 432, 489
FauI CCCGC 2 cut(s) 74, 154
Fnu4HI GCNGC 3 cut(s) 19, 86, 821
FokI GGATG 2 cut(s) 492, 671
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 3 cut(s) 19, 86, 821
FspBI CTAG 1 cut(s) 357
FspI TGCGCA 1 cut(s) 824
GlaI GCGC 3 cut(s) 22, 24, 824
GluI GCNGC 3 cut(s) 19, 86, 821
HaeIII GGCC 2 cut(s) 98, 529
HhaI GCGC 3 cut(s) 23, 25, 825
Hin1II CATG 2 cut(s) 436, 493
Hin6I GCGC 3 cut(s) 21, 23, 823
HinP1I GCGC 3 cut(s) 21, 23, 823
HindIII AAGCTT 1 cut(s) 469
HinfI GANTC 3 cut(s) 598, 679, 714
HphI GGTGA 3 cut(s) 233, 245, 251
Hpy166II GTNNAC 2 cut(s) 607, 800
Hpy188I TCNGA 8 cut(s) 42, 104, 117, 666, 684, 694, 779, 832
Hpy188III TCNNGA 3 cut(s) 302, 338, 593
Hpy8I GTNNAC 2 cut(s) 607, 800
HpyAV CCTTC 4 cut(s) 230, 278, 283, 751
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 616
HpyCH4V TGCA 4 cut(s) 34, 483, 499, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 309, 681
HpySE526I ACGT 1 cut(s) 616
Hsp92II CATG 2 cut(s) 436, 493
HspAI GCGC 3 cut(s) 21, 23, 823
Kzo9I GATC 5 cut(s) 298, 449, 538, 589, 774
LmnI GCTCC 4 cut(s) 117, 427, 551, 695
LpnPI CCDG 9 cut(s) 83, 173, 200, 321, 376, 408, 533, 641, 739
Lsp1109I GCAGC 2 cut(s) 5, 807
LweI GCATC 1 cut(s) 470
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 616
MaeIII GTNAC 2 cut(s) 504, 559
MalI GATC 5 cut(s) 300, 451, 540, 591, 776
MboI GATC 5 cut(s) 298, 449, 538, 589, 774
MboII GAAGA 4 cut(s) 218, 239, 254, 397
MfeI CAATTG 1 cut(s) 500
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 4 cut(s) 484, 500, 743, 814
MlyI GAGTC 2 cut(s) 592, 673
MmeI TCCRAC 1 cut(s) 691
MnlI CCTC 9 cut(s) 98, 194, 206, 223, 272, 364, 369, 375, 773
Mph1103I ATGCAT 1 cut(s) 676
MroXI GAANNNNTTC 1 cut(s) 293
MslI CAYNNNNRTG 2 cut(s) 437, 494
MspA1I CMGCKG 1 cut(s) 18
MspR9I CCNGG 1 cut(s) 188
MunI CAATTG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeII GATC 5 cut(s) 298, 449, 538, 589, 774
NlaIII CATG 2 cut(s) 436, 493
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 504, 559
NsbI TGCGCA 1 cut(s) 824
NsiI ATGCAT 1 cut(s) 676
PauI GCGCGC 1 cut(s) 21
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 1 cut(s) 714
PkrI GCNGC 3 cut(s) 20, 87, 822
Ple19I CGATCG 2 cut(s) 301, 452
PleI GAGTC 2 cut(s) 592, 673
PpsI GAGTC 2 cut(s) 592, 673
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 3 cut(s) 96, 107, 360
PsuI RGATCY 1 cut(s) 589
PteI GCGCGC 1 cut(s) 21
PvuI CGATCG 2 cut(s) 301, 452
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
RseI CAYNNNNRTG 2 cut(s) 437, 494
SatI GCNGC 3 cut(s) 19, 86, 821
Sau3AI GATC 5 cut(s) 298, 449, 538, 589, 774
Sau96I GGNCC 3 cut(s) 96, 107, 360
SchI GAGTC 2 cut(s) 592, 673
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 432
SetI ASST 9 cut(s) 20, 109, 122, 241, 362, 380, 473, 619, 692
SfaNI GCATC 1 cut(s) 470
SinI GGWCC 2 cut(s) 107, 360
SmiMI CAYNNNNRTG 2 cut(s) 437, 494
SmlI CTYRAG 2 cut(s) 593, 600
SmoI CTYRAG 2 cut(s) 593, 600
Sse9I AATT 4 cut(s) 484, 500, 743, 814
SsiI CCGC 3 cut(s) 67, 86, 161
SspI AATATT 1 cut(s) 583
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 619
TaqI TCGA 5 cut(s) 254, 297, 301, 448, 773
TasI AATT 4 cut(s) 484, 500, 743, 814
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 1 cut(s) 714
TscAI CASTG 3 cut(s) 83, 654, 758
TseFI GTSAC 2 cut(s) 504, 559
TseI GCWGC 2 cut(s) 18, 820
Tsp45I GTSAC 2 cut(s) 504, 559
TspDTI ATGAA 2 cut(s) 240, 506
TspRI CASTG 3 cut(s) 83, 654, 758
VpaK11BI GGWCC 2 cut(s) 107, 360
XapI RAATTY 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.