MD15G1202400.v1.1

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006686
Physical Location & Seq
Forward (+)
16119943 .. 16122664
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1202400.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCTTAATCTACACACCCGCTCACCCGTTCTTCAACCTCCCTGCACTCCCTTATCCATGTTTTCCCGCGACACAAACCGCTTCCCGCCATTTAGTACCGTTGGACTTCACTTTCAGAGCTTTTCTGCACAGCCGGGGCAGCTCAGGACCTCCGTATTCGTCGCAAAAGCGGGTCGGAGCCTAAGGCTGAAGACCCAGGAAGCGAGTGACGGAGAAGAAGATAAAGACGGCGGCTTTGTGGATATAGATTTCGAAGTTGCAGCTGAATCCGAAGACGGGTTTCCGGGTCGAACCGGCTTCGGAGGGAGAGAGGAAGAAAAGGACTTTGATCGAGATCCCGAGTTTGCTGAAATTATGGGTGCTTGTCTTGATGACCCAGAAAAAGCTAGGTCCAAGATGGAAGATAGGTTGAGGAAGAAAAGGAACAAGATTTTGCAGACAAAGTCTGGTTCAGGGCTGCCCATGAATGTGACATTCAACAACTTTGGTTTCTCAAACTCGTATATATGGTTTGAATTCTACAACACTCCGTTGGAGCAAGATGTTACCTTAATCTCTGATACCATTCGATCATGGCACATCATTGGGCGCATGGGTGGATGCAATTCCATGAATATGCAGTTGTCACAGTCTCCTTTTGATAAACGACCAAGTTATGATGCGATTCAGGGAGCAAATGTGACCCCAACTACTTTTTACAATATCGGAGATCTTGAGGTTCAGGATAACTTAGCACGTGTTTGGGTGGATATCGGCACCAGTGAACCATTGCTACTGGATGTTCTAATAAACGCATTGACTCAGATAAGCTCCGACTTTGTCGGAATCAAGCAAGTGGTGTTTGGTGGATCTGAATTTGAAAACTGGAAGGAGAACATGACATCAGAGGATGCAGGTTACAGCATCCACAACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

306

Amino Acids

34.17

Weight (kDa)

4.79

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 155 - 296 2.9e-53 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 884
AccB1I GGYRCC 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 21
AccII CGCG 1 cut(s) 69
AciI CCGC 6 cut(s) 19, 67, 79, 86, 170, 231
AclWI GGATC 2 cut(s) 329, 856
AcsI RAATTY 2 cut(s) 515, 854
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 737
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 276
AflIII ACRYGT 1 cut(s) 736
AgsI TTSAA 4 cut(s) 35, 478, 515, 860
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 5 cut(s) 120, 142, 263, 386, 810
AluI AGCT 5 cut(s) 120, 142, 263, 386, 810
Alw26I GTCTC 1 cut(s) 636
AlwI GGATC 2 cut(s) 329, 856
Ama87I CYCGRG 1 cut(s) 338
ApeKI GCWGC 3 cut(s) 139, 260, 457
ApoI RAATTY 2 cut(s) 515, 854
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 2 cut(s) 147, 390
AsuC2I CCSGG 2 cut(s) 135, 285
AsuHPI GGTGA 1 cut(s) 15
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 147, 390
AxyI CCTNAGG 1 cut(s) 182
BanI GGYRCC 1 cut(s) 755
BbrPI CACGTG 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 197, 279
BbvI GCAGC 3 cut(s) 151, 272, 444
BccI CCATC 1 cut(s) 391
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 1 cut(s) 197
BcnI CCSGG 2 cut(s) 135, 285
BcoDI GTCTC 1 cut(s) 636
BfaI CTAG 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 884
BglII AGATCT 1 cut(s) 709
BisI GCNGC 4 cut(s) 140, 232, 261, 458
BlsI GCNGC 4 cut(s) 141, 233, 262, 459
Bme1390I CCNGG 3 cut(s) 135, 197, 285
Bme18I GGWCC 2 cut(s) 147, 390
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 147, 390
BmiI GGNNCC 2 cut(s) 179, 757
BmrFI CCNGG 3 cut(s) 135, 197, 285
BmsI GCATC 4 cut(s) 590, 649, 880, 912
BpiI GAAGAC 2 cut(s) 197, 279
Bpu10I CCTNAGC 1 cut(s) 143
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 2 cut(s) 135, 285
BsaAI YACGTR 1 cut(s) 737
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 134, 195
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 3 cut(s) 759, 780, 869
Bse21I CCTNAGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 767
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 134, 195
BseGI GGATG 4 cut(s) 605, 784, 895, 903
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMI GCAATG 1 cut(s) 767
BseMII CTCAG 2 cut(s) 157, 815
BseNI ACTGG 3 cut(s) 759, 780, 869
BseXI GCAGC 3 cut(s) 151, 272, 444
BsgI GTGCAG 2 cut(s) 28, 111
Bsh1236I CGCG 1 cut(s) 69
BshNI GGYRCC 1 cut(s) 755
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 3 cut(s) 134, 284, 294
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 1 cut(s) 636
BsoBI CYCGRG 1 cut(s) 338
Bsp119I TTCGAA 1 cut(s) 252
Bsp143I GATC 5 cut(s) 328, 334, 569, 709, 848
BspACI CCGC 6 cut(s) 19, 67, 79, 86, 170, 231
BspCNI CTCAG 2 cut(s) 156, 814
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 179, 757
BspMI ACCTGC 1 cut(s) 884
BspPI GGATC 2 cut(s) 329, 856
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 767
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 3 cut(s) 759, 780, 869
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 2 cut(s) 134, 195
BssMI GATC 5 cut(s) 328, 334, 569, 709, 848
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 100, 630
BstBAI YACGTR 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 252
BstDEI CTNAG 4 cut(s) 143, 182, 730, 801
BstF5I GGATG 4 cut(s) 605, 784, 895, 903
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 5 cut(s) 331, 337, 572, 712, 851
BstMAI GTCTC 1 cut(s) 636
BstMBI GATC 5 cut(s) 328, 334, 569, 709, 848
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 133, 195, 283
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 3 cut(s) 151, 272, 444
BstV2I GAAGAC 2 cut(s) 197, 279
BstX2I RGATCY 3 cut(s) 334, 709, 848
BstYI RGATCY 3 cut(s) 334, 709, 848
Bsu36I CCTNAGG 1 cut(s) 182
BtsCI GGATG 4 cut(s) 605, 784, 895, 903
BtsIMutI CAGTG 1 cut(s) 766
BveI ACCTGC 1 cut(s) 884
CfoI GCGC 1 cut(s) 591
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 147, 390
Csp6I GTAC 1 cut(s) 96
CviAII CATG 6 cut(s) 58, 463, 573, 592, 610, 877
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 4 cut(s) 143, 182, 730, 801
DpnI GATC 5 cut(s) 330, 336, 571, 711, 850
DpnII GATC 5 cut(s) 328, 334, 569, 709, 848
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 147, 390
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 737
Eco81I CCTNAGG 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 195
EcoRV GATATC 1 cut(s) 751
FaeI CATG 6 cut(s) 61, 466, 576, 595, 613, 880
FatI CATG 6 cut(s) 57, 462, 572, 591, 609, 876
FauI CCCGC 4 cut(s) 26, 74, 93, 163
Fnu4HI GCNGC 4 cut(s) 140, 232, 261, 458
FokI GGATG 4 cut(s) 612, 791, 890, 902
Fsp4HI GCNGC 4 cut(s) 140, 232, 261, 458
FspBI CTAG 1 cut(s) 387
GlaI GCGC 1 cut(s) 590
GluI GCNGC 4 cut(s) 140, 232, 261, 458
HapII CCGG 3 cut(s) 134, 284, 294
HhaI GCGC 1 cut(s) 591
Hin1II CATG 6 cut(s) 61, 466, 576, 595, 613, 880
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HinfI GANTC 4 cut(s) 266, 664, 799, 825
HpaII CCGG 3 cut(s) 134, 284, 294
HphI GGTGA 1 cut(s) 15
Hpy166II GTNNAC 1 cut(s) 764
Hpy188III TCNNGA 6 cut(s) 145, 332, 338, 368, 713, 722
Hpy8I GTNNAC 1 cut(s) 764
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 862
HpyCH4III ACNGT 2 cut(s) 100, 630
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 7 cut(s) 45, 128, 260, 436, 603, 619, 893
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 4 cut(s) 143, 182, 730, 801
HpySE526I ACGT 1 cut(s) 736
Hsp92II CATG 6 cut(s) 61, 466, 576, 595, 613, 880
HspAI GCGC 1 cut(s) 589
Kzo9I GATC 5 cut(s) 328, 334, 569, 709, 848
LmnI GCTCC 4 cut(s) 177, 535, 671, 815
Lsp1109I GCAGC 3 cut(s) 151, 272, 444
LweI GCATC 4 cut(s) 590, 649, 880, 912
MaeI CTAG 1 cut(s) 387
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 6 cut(s) 206, 469, 544, 624, 679, 896
MalI GATC 5 cut(s) 330, 336, 571, 711, 850
MbiI CCGCTC 1 cut(s) 21
MboI GATC 5 cut(s) 328, 334, 569, 709, 848
MboII GAAGA 8 cut(s) 23, 202, 227, 230, 284, 326, 413, 427
MflI RGATCY 3 cut(s) 334, 709, 848
MluCI AATT 4 cut(s) 351, 515, 604, 854
MlyI GAGTC 1 cut(s) 793
MmeI TCCRAC 5 cut(s) 82, 155, 513, 802, 837
MnlI CCTC 7 cut(s) 48, 160, 296, 304, 405, 709, 880
MseI TTAA 2 cut(s) 6, 551
MslI CAYNNNNRTG 2 cut(s) 467, 614
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 3 cut(s) 134, 284, 294
MspR9I CCNGG 3 cut(s) 135, 197, 285
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 139
NciI CCSGG 2 cut(s) 135, 285
NdeII GATC 5 cut(s) 328, 334, 569, 709, 848
NlaIII CATG 6 cut(s) 61, 466, 576, 595, 613, 880
NlaIV GGNNCC 2 cut(s) 179, 757
NmuCI GTSAC 4 cut(s) 206, 469, 624, 679
NspV TTCGAA 1 cut(s) 252
PfeI GAWTC 3 cut(s) 266, 664, 825
PflFI GACNNNGTC 2 cut(s) 442, 818
PkrI GCNGC 4 cut(s) 141, 233, 262, 459
PleI GAGTC 1 cut(s) 793
PmaCI CACGTG 1 cut(s) 737
PmlI CACGTG 1 cut(s) 737
PpsI GAGTC 1 cut(s) 793
Ppu21I YACGTR 1 cut(s) 737
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 195
PspCI CACGTG 1 cut(s) 737
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 179, 757
PspPI GGNCC 2 cut(s) 147, 390
PspPPI RGGWCCY 1 cut(s) 147
PsrI GAACNNNNNNTAC 2 cut(s) 756, 788
PsuI RGATCY 3 cut(s) 334, 709, 848
PsyI GACNNNGTC 2 cut(s) 442, 818
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 467, 614
SaqAI TTAA 2 cut(s) 6, 551
SatI GCNGC 4 cut(s) 140, 232, 261, 458
Sau3AI GATC 5 cut(s) 328, 334, 569, 709, 848
Sau96I GGNCC 2 cut(s) 147, 390
SchI GAGTC 1 cut(s) 793
ScrFI CCNGG 3 cut(s) 135, 197, 285
SfaNI GCATC 4 cut(s) 590, 649, 880, 912
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 2 cut(s) 147, 390
SmiMI CAYNNNNRTG 2 cut(s) 467, 614
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 4 cut(s) 351, 515, 604, 854
SsiI CCGC 6 cut(s) 19, 67, 79, 86, 170, 231
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 3 cut(s) 133, 195, 283
TaaI ACNGT 2 cut(s) 100, 630
TaiI ACGT 1 cut(s) 739
TaqI TCGA 4 cut(s) 252, 289, 331, 568
TasI AATT 4 cut(s) 351, 515, 604, 854
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 3 cut(s) 266, 664, 825
Tru1I TTAA 2 cut(s) 6, 551
Tru9I TTAA 2 cut(s) 6, 551
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 4 cut(s) 206, 469, 624, 679
TseI GCWGC 3 cut(s) 139, 260, 457
Tsp45I GTSAC 4 cut(s) 206, 469, 624, 679
TspDTI ATGAA 2 cut(s) 479, 626
TspGWI ACGGA 3 cut(s) 142, 225, 519
TspRI CASTG 1 cut(s) 766
Tth111I GACNNNGTC 2 cut(s) 442, 818
VpaK11BI GGWCC 2 cut(s) 147, 390
XapI RAATTY 2 cut(s) 515, 854
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.