Rw2G005940

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006686
Physical Location & Seq
Reverse (-)
5665315 .. 5667511
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005940.1

Sequence Viewer

Length: 861 bp
ATGGCTGTCTTACACAGCTGCGCGCCTTTTCTGCAAAGTCCGAGCATTCCCTTATCCTTGTTTTCCCGCCAGAAACACTGCTCGCCACCGTTTTGGGCCGTCGGAGGTCCATTTCGGAGCTGTTATGGTCAGCCCAAGACGAGTGTATTGGTGGCTAAAGCGGGTCGGGGTCTAAAGTTGGGGACCCAGAAAGGGAGTGGAGGAGAAGATGGAGGCTTTGGTGATGAAGGTGAAGATGGGTTTTCGGGTCGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTTGATCGAGACCCTGAGTTTGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAAAAGGAACAAAATATTGCATACAAAATCGGGGTCAGGGGAACCCATGAGAGTAGTATTCAATGACTTTGGTTTCTCAAATTCATACATATGGTTCGAGTTCTACAACATTCCATTGGAACAAGATACTATTCGATCGTGGCACATTATTGGACGCTTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAAACCCCTTTGGATAAACGACCAAGTTATGATCCCATTCAGGGAGCAAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGATTCAAGACAACTTGGGACGTGTTTGGGTGGATATCGGTACTAAAGAGCCACTGATACTGGATGTTCTGATTAATGCATTGACTCAGATAAGCTCCGACTTTGTTGGAATCAAGCAAGTGGTGTTTGGTGGAACTGAATTTGAACACTGGAAGGAGAACATGACATCAGAGGAAGTTGGTTATACTGTTCACAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

286

Amino Acids

31.74

Weight (kDa)

6.11

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 142 - 277 9e-47 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 67, 161
AclWI GGATC 1 cut(s) 596
AcsI RAATTY 2 cut(s) 457, 797
AfaI GTAC 1 cut(s) 700
AfiI CCNNNNNNNGG 3 cut(s) 192, 343, 611
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 3 cut(s) 439, 665, 803
AjiI CACGTC 1 cut(s) 680
AluBI AGCT 3 cut(s) 18, 120, 753
AluI AGCT 3 cut(s) 18, 120, 753
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 596
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 457, 797
ArsI GACNNNNNNTTYG 4 cut(s) 287, 319, 395, 427
AseI ATTAAT 1 cut(s) 732
AspLEI GCGC 2 cut(s) 23, 25
AspS9I GGNCC 4 cut(s) 96, 107, 183, 351
AsuHPI GGTGA 2 cut(s) 233, 242
AvaII GGWCC 3 cut(s) 107, 183, 351
BbvI GCAGC 1 cut(s) 5
BccI CCATC 3 cut(s) 203, 230, 352
BceAI ACGGC 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 348
BglII AGATCT 1 cut(s) 652
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme18I GGWCC 3 cut(s) 107, 183, 351
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 4 cut(s) 96, 107, 183, 351
BmiI GGNNCC 3 cut(s) 184, 185, 420
BmsI GCATC 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 677
BsaI GGTCTC 1 cut(s) 288
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 606, 636
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 343, 611
Bse1I ACTGG 2 cut(s) 723, 812
BseGI GGATG 2 cut(s) 548, 727
BseLI CCNNNNNNNGG 3 cut(s) 192, 343, 611
BseMII CTCAG 2 cut(s) 291, 758
BseNI ACTGG 2 cut(s) 723, 812
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 216, 285, 377
BseXI GCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 515
BshFI GGCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 515
BslFI GGGAC 2 cut(s) 196, 690
BslI CCNNNNNNNGG 3 cut(s) 192, 343, 611
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 2 cut(s) 196, 690
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 1 cut(s) 288
Bsp143I GATC 4 cut(s) 289, 512, 601, 652
BspACI CCGC 2 cut(s) 67, 161
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 292, 757
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 184, 185, 420
BspPI GGATC 1 cut(s) 596
BspTNI GGTCTC 1 cut(s) 288
BsrI ACTGG 2 cut(s) 723, 812
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 4 cut(s) 289, 512, 601, 652
Bst4CI ACNGT 2 cut(s) 90, 847
BstC8I GCNNGC 2 cut(s) 23, 83
BstDEI CTNAG 2 cut(s) 300, 744
BstF5I GGATG 2 cut(s) 548, 727
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 25
BstKTI GATC 4 cut(s) 292, 515, 604, 655
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 289, 512, 601, 652
BstMCI CGRYCG 1 cut(s) 515
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 652
BstXI CCANNNNNNTGG 1 cut(s) 93
BstYI RGATCY 1 cut(s) 652
BsuRI GGCC 1 cut(s) 98
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 2 cut(s) 548, 727
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 710, 805
Cac8I GCNNGC 2 cut(s) 23, 83
CfoI GCGC 2 cut(s) 23, 25
Cfr13I GGNCC 4 cut(s) 96, 107, 183, 351
CseI GACGC 1 cut(s) 540
Csp6I GTAC 1 cut(s) 699
CviAII CATG 3 cut(s) 424, 553, 820
CviQI GTAC 1 cut(s) 699
DdeI CTNAG 2 cut(s) 300, 744
DpnI GATC 4 cut(s) 291, 514, 603, 654
DpnII GATC 4 cut(s) 289, 512, 601, 652
Eco31I GGTCTC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 3 cut(s) 107, 183, 351
EcoO109I RGGNCCY 1 cut(s) 183
EcoRV GATATC 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 739
FaeI CATG 3 cut(s) 427, 556, 823
FaqI GGGAC 2 cut(s) 196, 690
FatI CATG 3 cut(s) 423, 552, 819
FauI CCCGC 2 cut(s) 74, 154
FauNDI CATATG 1 cut(s) 467
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 2 cut(s) 555, 734
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 1 cut(s) 348
GlaI GCGC 2 cut(s) 22, 24
GluI GCNGC 1 cut(s) 19
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 540
HhaI GCGC 2 cut(s) 23, 25
Hin1II CATG 3 cut(s) 427, 556, 823
Hin6I GCGC 2 cut(s) 21, 23
HinP1I GCGC 2 cut(s) 21, 23
HinfI GANTC 3 cut(s) 661, 742, 768
HphI GGTGA 2 cut(s) 233, 242
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 8 cut(s) 42, 104, 117, 252, 729, 747, 757, 829
Hpy188III TCNNGA 4 cut(s) 293, 329, 656, 665
Hpy8I GTNNAC 1 cut(s) 850
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 221, 269, 274, 805
HpyCH4III ACNGT 2 cut(s) 90, 847
HpyCH4IV ACGT 1 cut(s) 679
HpyCH4V TGCA 5 cut(s) 34, 397, 546, 562, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 300, 744
HpySE526I ACGT 1 cut(s) 679
Hsp92II CATG 3 cut(s) 427, 556, 823
HspAI GCGC 2 cut(s) 21, 23
KflI GGGWCCC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 289, 512, 601, 652
LmnI GCTCC 3 cut(s) 117, 614, 758
LpnPI CCDG 7 cut(s) 83, 200, 312, 399, 596, 704, 793
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 1 cut(s) 533
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 679
MaeIII GTNAC 2 cut(s) 567, 622
MalI GATC 4 cut(s) 291, 514, 603, 654
MboI GATC 4 cut(s) 289, 512, 601, 652
MboII GAAGA 3 cut(s) 218, 245, 388
MfeI CAATTG 1 cut(s) 563
MflI RGATCY 1 cut(s) 652
MluCI AATT 4 cut(s) 457, 547, 563, 797
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 4 cut(s) 82, 230, 745, 780
MnlI CCTC 8 cut(s) 98, 194, 206, 263, 355, 360, 366, 823
Mph1103I ATGCAT 1 cut(s) 739
MseI TTAA 1 cut(s) 732
MslI CAYNNNNRTG 2 cut(s) 466, 557
MspA1I CMGCKG 1 cut(s) 18
MunI CAATTG 1 cut(s) 563
Mva1269I GAATGC 1 cut(s) 45
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeI CATATG 1 cut(s) 467
NdeII GATC 4 cut(s) 289, 512, 601, 652
NlaIII CATG 3 cut(s) 427, 556, 823
NlaIV GGNNCC 3 cut(s) 184, 185, 420
NmuCI GTSAC 2 cut(s) 567, 622
NsiI ATGCAT 1 cut(s) 739
PauI GCGCGC 1 cut(s) 21
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 661, 768
PkrI GCNGC 1 cut(s) 20
Ple19I CGATCG 1 cut(s) 515
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PpuMI RGGWCCY 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 732
Psp5II RGGWCCY 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 184, 185, 420
PspPI GGNCC 4 cut(s) 96, 107, 183, 351
PspPPI RGGWCCY 1 cut(s) 183
PsuI RGATCY 1 cut(s) 652
PteI GCGCGC 1 cut(s) 21
PvuI CGATCG 1 cut(s) 515
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 1 cut(s) 700
RsaNI GTAC 1 cut(s) 699
RseI CAYNNNNRTG 2 cut(s) 466, 557
SaqAI TTAA 1 cut(s) 732
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 4 cut(s) 289, 512, 601, 652
Sau96I GGNCC 4 cut(s) 96, 107, 183, 351
SchI GAGTC 1 cut(s) 736
SetI ASST 8 cut(s) 20, 109, 122, 232, 353, 371, 682, 755
SfaNI GCATC 1 cut(s) 533
SinI GGWCC 3 cut(s) 107, 183, 351
SmiMI CAYNNNNRTG 2 cut(s) 466, 557
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
Sse9I AATT 4 cut(s) 457, 547, 563, 797
SsiI CCGC 2 cut(s) 67, 161
SspI AATATT 2 cut(s) 393, 646
SspMI CTAG 1 cut(s) 348
TaaI ACNGT 2 cut(s) 90, 847
TaiI ACGT 1 cut(s) 682
TaqI TCGA 3 cut(s) 292, 474, 511
TasI AATT 4 cut(s) 457, 547, 563, 797
TfiI GAWTC 2 cut(s) 661, 768
Tru1I TTAA 1 cut(s) 732
Tru9I TTAA 1 cut(s) 732
TscAI CASTG 3 cut(s) 83, 717, 812
TseFI GTSAC 2 cut(s) 567, 622
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 2 cut(s) 567, 622
TspDTI ATGAA 3 cut(s) 240, 450, 569
TspRI CASTG 3 cut(s) 83, 717, 812
VpaK11BI GGWCC 3 cut(s) 107, 183, 351
VspI ATTAAT 1 cut(s) 732
XapI RAATTY 2 cut(s) 457, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.