FvH4_1g06800

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006686
Physical Location & Seq
Forward (+)
3581531 .. 3584206
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06800.t3

Sequence Viewer

Length: 846 bp
ATGGCTCTCCTACACTCATGTTCACCTTTTCTTCATACAAGCGTTTGCTTATCTGTACTTTCCCGCCAGAAACACTGCCAGCCGCCGGTTTGGGCCGTCGGAGGTCAGACCAAGACGTGTGTATTGGTTTGTAAAGCTGGTGGGGGAGTGAAGTTTGATACCCAGAAAGAGAGTGAAGGAGAAGATGGAGGGTTTGGTGAAGATGGGTTTTCGAGTAGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTTGATAGAGACCCTGAGTTTGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAAAAGGAACAAGATATTGCATACAAAATCTGGTTCAGGGGAACCCATGAGAGTTGTATTCAATGACTTTAGTTTCTCAAATTCATACATATGGTTCGAGTTCTACAACACTCCATTGGAACAAGATGTTTCCTTAATCTCGGATACTGTTCGATCATGGCACATCATTGGACGCTTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAATCCCCTTTGGATAAACGACCAAGTTATGATCCCATTCAGGGAGCGAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGATTCAAGACAACTTGGGACGTGTTTGGGTGGATATCGGTACTAAGGAGCCACTAATACTGGATGTTCTGATAAATGCATTGACTCAGATAAGCTCCGACTTTGTTGGGATCAAGAAAGTGGTGTTTGGTGGAACTGAATTTGAACACTGGAAGGAGAACTTGACGTCAGAAGAAGCAGGTTATACTGTTCACAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

282

Amino Acids

31.28

Weight (kDa)

5.7

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 131 - 225 7.5e-30 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 812
AciI CCGC 2 cut(s) 64, 83
AclWI GGATC 2 cut(s) 581, 761
AcsI RAATTY 2 cut(s) 424, 782
AcyI GRCGYC 1 cut(s) 809
AfaI GTAC 2 cut(s) 57, 685
AfiI CCNNNNNNNGG 3 cut(s) 85, 310, 596
AflIII ACRYGT 2 cut(s) 116, 664
AgsI TTSAA 3 cut(s) 406, 650, 788
AjiI CACGTC 2 cut(s) 117, 665
AluBI AGCT 2 cut(s) 137, 738
AluI AGCT 2 cut(s) 137, 738
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 2 cut(s) 581, 761
AoxI GGCC 1 cut(s) 93
ApoI RAATTY 2 cut(s) 424, 782
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AspS9I GGNCC 2 cut(s) 93, 318
AsuHPI GGTGA 2 cut(s) 15, 209
AvaII GGWCC 1 cut(s) 318
BccI CCATC 3 cut(s) 179, 197, 319
BceAI ACGGC 1 cut(s) 80
BciVI GTATCC 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 315
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 481
BglII AGATCT 1 cut(s) 637
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 318
BmgBI CACGTC 2 cut(s) 117, 665
BmgT120I GGNCC 2 cut(s) 93, 318
BmiI GGNNCC 2 cut(s) 387, 693
BmsI GCATC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 809
BsaI GGTCTC 1 cut(s) 255
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 591, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 310, 596
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 708, 797
BseGI GGATG 2 cut(s) 533, 712
BseLI CCNNNNNNNGG 3 cut(s) 85, 310, 596
BseMII CTCAG 2 cut(s) 258, 743
BseNI ACTGG 2 cut(s) 708, 797
BseRI GAGGAG 2 cut(s) 252, 344
BshFI GGCC 1 cut(s) 95
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 675
BslI CCNNNNNNNGG 3 cut(s) 85, 310, 596
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 675
BsnI GGCC 1 cut(s) 95
Bso31I GGTCTC 1 cut(s) 255
Bsp143I GATC 4 cut(s) 497, 586, 637, 753
BspACI CCGC 2 cut(s) 64, 83
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 2 cut(s) 259, 742
BspLI GGNNCC 2 cut(s) 387, 693
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 2 cut(s) 581, 761
BspTNI GGTCTC 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 2 cut(s) 708, 797
BssAI RCCGGY 1 cut(s) 85
BssMI GATC 4 cut(s) 497, 586, 637, 753
BssNI GRCGYC 1 cut(s) 809
Bst4CI ACNGT 2 cut(s) 493, 832
BstACI GRCGYC 1 cut(s) 809
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 3 cut(s) 267, 687, 729
BstF5I GGATG 2 cut(s) 533, 712
BstKTI GATC 4 cut(s) 500, 589, 640, 756
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 4 cut(s) 497, 586, 637, 753
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 1 cut(s) 95
BtrI CACGTC 2 cut(s) 117, 665
BtsCI GGATG 2 cut(s) 533, 712
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 790
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 2 cut(s) 93, 318
CseI GACGC 1 cut(s) 525
Csp6I GTAC 2 cut(s) 56, 684
CviAII CATG 4 cut(s) 18, 391, 501, 538
CviJI RGCY 7 cut(s) 5, 82, 95, 137, 314, 694, 738
CviKI_1 RGCY 7 cut(s) 5, 82, 95, 137, 314, 694, 738
CviQI GTAC 2 cut(s) 56, 684
DdeI CTNAG 3 cut(s) 267, 687, 729
DpnI GATC 4 cut(s) 499, 588, 639, 755
DpnII GATC 4 cut(s) 497, 586, 637, 753
Eco31I GGTCTC 1 cut(s) 255
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 318
EcoRV GATATC 1 cut(s) 679
EcoT22I ATGCAT 1 cut(s) 724
FaeI CATG 4 cut(s) 21, 394, 504, 541
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FaqI GGGAC 1 cut(s) 675
FatI CATG 4 cut(s) 17, 390, 500, 537
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 434
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 2 cut(s) 540, 719
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 1 cut(s) 315
GluI GCNGC 1 cut(s) 83
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 86
HgaI GACGC 1 cut(s) 525
Hin1I GRCGYC 1 cut(s) 809
Hin1II CATG 4 cut(s) 21, 394, 504, 541
HinfI GANTC 2 cut(s) 646, 727
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 15, 209
Hpy166II GTNNAC 2 cut(s) 23, 835
Hpy188I TCNGA 7 cut(s) 101, 108, 487, 714, 732, 742, 814
Hpy188III TCNNGA 4 cut(s) 296, 641, 650, 757
Hpy8I GTNNAC 2 cut(s) 23, 835
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 4 cut(s) 170, 236, 241, 790
HpyCH4III ACNGT 2 cut(s) 493, 832
HpyCH4IV ACGT 3 cut(s) 116, 664, 809
HpyCH4V TGCA 4 cut(s) 364, 531, 547, 722
HpyF3I CTNAG 3 cut(s) 267, 687, 729
HpySE526I ACGT 3 cut(s) 116, 664, 809
Hsp92I GRCGYC 1 cut(s) 809
Hsp92II CATG 4 cut(s) 21, 394, 504, 541
Kzo9I GATC 4 cut(s) 497, 586, 637, 753
LmnI GCTCC 3 cut(s) 599, 691, 743
LweI GCATC 1 cut(s) 518
MaeI CTAG 1 cut(s) 315
MaeII ACGT 3 cut(s) 116, 664, 809
MaeIII GTNAC 2 cut(s) 552, 607
MalI GATC 4 cut(s) 499, 588, 639, 755
MboI GATC 4 cut(s) 497, 586, 637, 753
MboII GAAGA 5 cut(s) 23, 194, 212, 355, 827
MfeI CAATTG 1 cut(s) 548
MflI RGATCY 1 cut(s) 637
MluCI AATT 4 cut(s) 424, 532, 548, 782
MlyI GAGTC 1 cut(s) 721
MmeI TCCRAC 2 cut(s) 79, 765
MnlI CCTC 6 cut(s) 95, 182, 230, 322, 327, 333
Mph1103I ATGCAT 1 cut(s) 724
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 2 cut(s) 433, 542
MspI CCGG 1 cut(s) 86
MunI CAATTG 1 cut(s) 548
NdeI CATATG 1 cut(s) 434
NdeII GATC 4 cut(s) 497, 586, 637, 753
NlaIII CATG 4 cut(s) 21, 394, 504, 541
NlaIV GGNNCC 2 cut(s) 387, 693
NmuCI GTSAC 2 cut(s) 552, 607
NsiI ATGCAT 1 cut(s) 724
PfeI GAWTC 1 cut(s) 646
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
PspN4I GGNNCC 2 cut(s) 387, 693
PspPI GGNCC 2 cut(s) 93, 318
PsuI RGATCY 1 cut(s) 637
RsaI GTAC 2 cut(s) 57, 685
RsaNI GTAC 2 cut(s) 56, 684
RseI CAYNNNNRTG 2 cut(s) 433, 542
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 4 cut(s) 497, 586, 637, 753
Sau96I GGNCC 2 cut(s) 93, 318
SchI GAGTC 1 cut(s) 721
SfaNI GCATC 1 cut(s) 518
SinI GGWCC 1 cut(s) 318
SmiMI CAYNNNNRTG 2 cut(s) 433, 542
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 4 cut(s) 424, 532, 548, 782
SsiI CCGC 2 cut(s) 64, 83
SspI AATATT 1 cut(s) 631
SspMI CTAG 1 cut(s) 315
TaaI ACNGT 2 cut(s) 493, 832
TaiI ACGT 3 cut(s) 119, 667, 812
TaqI TCGA 3 cut(s) 212, 441, 496
TasI AATT 4 cut(s) 424, 532, 548, 782
TatI WGTACW 1 cut(s) 55
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 1 cut(s) 646
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 2 cut(s) 80, 797
TseFI GTSAC 2 cut(s) 552, 607
Tsp45I GTSAC 2 cut(s) 552, 607
TspDTI ATGAA 3 cut(s) 23, 417, 554
TspRI CASTG 2 cut(s) 80, 797
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 2 cut(s) 424, 782
XspI CTAG 1 cut(s) 315
ZraI GACGTC 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.