Rh2AG067100

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006686
Physical Location & Seq
Forward (+)
5580691 .. 5583042
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG067100.1

Sequence Viewer

Length: 888 bp
ATGGCTGTCTTACACAGCTGCGCGCCTTTTCTGCAAACTCCGAGGGTTCCCTTATCCTTATTTTCCCGCCAGAAACACTGCTCGCCGCCGTTTTGGGCCGTCGGAGGTCCATTTCGGAGCTGTAATGGTCAGCCCAAGACGAGTGTATTGGTGGCTAAAGCGGGTCGGCGTTTAAAGTTGGGGACCCAGAAAGAGAGTGAAGGAGAAGATGGAGGCTTTGGTGATGAAGAGGGTGAAGGTGAAGATGGGTTTTCGAGTGGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTTGATCGAGACCCTGAGTTTGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAAAAGGAACAAGATATTGCATACAAAATCGGGGTCAGGGGAACCCATGAGAGTGGTATTCAATGACTATGGATTCTCAAATTCATACATATGGTTCGAGTTCTACAACATTCCCTTGGAACAAGATGTTTCCTTAATCTCGGATACTATTCGATCGTGGCACATTATTGGACGCTTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAATCCCCTTTGGATAAACGACCAAGTTATGATCCCATTCAGGGAGCAAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGATTCAAGACAACTTGGGACGTGTTTGGGTGGATATCGGTACTAAAGAGCCACTGATACTGGATGTTCTGATAAATGCATTGAGTCAGATAAGCTCCGACTTTGTTGGAATCAAGCAAGTGGTGTTTGGTGGAACTGAATTTGAACACTGGAAGGAGAACATGACATCAGAGGAAGTTGGATATACTGTTCACAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

295

Amino Acids

32.85

Weight (kDa)

5.49

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 145 - 286 3.9e-51 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 67, 86, 161
AclWI GGATC 1 cut(s) 623
AcsI RAATTY 2 cut(s) 466, 824
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 2 cut(s) 352, 638
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 3 cut(s) 448, 692, 830
AjiI CACGTC 1 cut(s) 707
AloI GAACNNNNNNTCC 1 cut(s) 858
AluBI AGCT 3 cut(s) 18, 120, 780
AluI AGCT 3 cut(s) 18, 120, 780
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 1 cut(s) 623
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 466, 824
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 404, 436
AspLEI GCGC 2 cut(s) 23, 25
AspS9I GGNCC 4 cut(s) 96, 107, 183, 360
AsuHPI GGTGA 3 cut(s) 233, 245, 251
AvaII GGWCC 3 cut(s) 107, 183, 360
BbvI GCAGC 1 cut(s) 5
BccI CCATC 3 cut(s) 203, 239, 361
BceAI ACGGC 2 cut(s) 73, 83
BciVI GTATCC 1 cut(s) 523
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 1 cut(s) 357
BfuI GTATCC 1 cut(s) 523
BglII AGATCT 1 cut(s) 679
BisI GCNGC 2 cut(s) 19, 86
BlsI GCNGC 2 cut(s) 20, 87
Bme18I GGWCC 3 cut(s) 107, 183, 360
BmgBI CACGTC 1 cut(s) 707
BmgT120I GGNCC 4 cut(s) 96, 107, 183, 360
BmiI GGNNCC 4 cut(s) 48, 184, 185, 429
BmsI GCATC 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 704
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 41, 501
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 633, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 638
Bse1I ACTGG 2 cut(s) 750, 839
BseDI CCNNGG 2 cut(s) 41, 501
BseGI GGATG 2 cut(s) 575, 754
BseLI CCNNNNNNNGG 2 cut(s) 352, 638
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 750, 839
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 2 cut(s) 294, 386
BseXI GCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 542
BslFI GGGAC 2 cut(s) 196, 717
BslI CCNNNNNNNGG 2 cut(s) 352, 638
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 2 cut(s) 196, 717
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 1 cut(s) 297
Bsp143I GATC 4 cut(s) 298, 539, 628, 679
BspACI CCGC 3 cut(s) 67, 86, 161
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 4 cut(s) 48, 184, 185, 429
BspPI GGATC 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 297
BsrI ACTGG 2 cut(s) 750, 839
BssECI CCNNGG 2 cut(s) 41, 501
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 4 cut(s) 298, 539, 628, 679
BssT1I CCWWGG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 23, 83
BstDEI CTNAG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 575, 754
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 25
BstKTI GATC 4 cut(s) 301, 542, 631, 682
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 298, 539, 628, 679
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 679
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 1 cut(s) 98
BtrI CACGTC 1 cut(s) 707
BtsCI GGATG 2 cut(s) 575, 754
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 737, 832
Cac8I GCNNGC 2 cut(s) 23, 83
CfoI GCGC 2 cut(s) 23, 25
Cfr13I GGNCC 4 cut(s) 96, 107, 183, 360
CseI GACGC 1 cut(s) 567
Csp6I GTAC 1 cut(s) 726
CviAII CATG 3 cut(s) 433, 580, 847
CviQI GTAC 1 cut(s) 726
DdeI CTNAG 1 cut(s) 309
DpnI GATC 4 cut(s) 300, 541, 630, 681
DpnII GATC 4 cut(s) 298, 539, 628, 679
DraI TTTAAA 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 3 cut(s) 107, 183, 360
EcoO109I RGGNCCY 1 cut(s) 183
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 766
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 3 cut(s) 436, 583, 850
FaqI GGGAC 2 cut(s) 196, 717
FatI CATG 3 cut(s) 432, 579, 846
FauI CCCGC 2 cut(s) 74, 154
FauNDI CATATG 1 cut(s) 476
Fnu4HI GCNGC 2 cut(s) 19, 86
FokI GGATG 2 cut(s) 582, 761
Fsp4HI GCNGC 2 cut(s) 19, 86
FspBI CTAG 1 cut(s) 357
GlaI GCGC 2 cut(s) 22, 24
GluI GCNGC 2 cut(s) 19, 86
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 567
HhaI GCGC 2 cut(s) 23, 25
Hin1II CATG 3 cut(s) 436, 583, 850
Hin6I GCGC 2 cut(s) 21, 23
HinP1I GCGC 2 cut(s) 21, 23
HinfI GANTC 4 cut(s) 459, 688, 769, 795
HphI GGTGA 3 cut(s) 233, 245, 251
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 8 cut(s) 42, 104, 117, 529, 756, 774, 784, 856
Hpy188III TCNNGA 4 cut(s) 302, 338, 683, 692
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 5 cut(s) 194, 230, 278, 283, 832
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 5 cut(s) 34, 406, 573, 589, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 706
Hsp92II CATG 3 cut(s) 436, 583, 850
HspAI GCGC 2 cut(s) 21, 23
KflI GGGWCCC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 298, 539, 628, 679
LmnI GCTCC 3 cut(s) 117, 641, 785
LpnPI CCDG 7 cut(s) 83, 200, 321, 408, 623, 731, 820
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 1 cut(s) 560
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 594, 649
MalI GATC 4 cut(s) 300, 541, 630, 681
MboI GATC 4 cut(s) 298, 539, 628, 679
MboII GAAGA 4 cut(s) 218, 239, 254, 397
MfeI CAATTG 1 cut(s) 590
MflI RGATCY 1 cut(s) 679
MluCI AATT 4 cut(s) 466, 574, 590, 824
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 4 cut(s) 82, 772, 807, 844
MnlI CCTC 9 cut(s) 36, 98, 206, 223, 272, 364, 369, 375, 850
Mph1103I ATGCAT 1 cut(s) 766
MseI TTAA 2 cut(s) 173, 521
MslI CAYNNNNRTG 3 cut(s) 437, 475, 584
MspA1I CMGCKG 1 cut(s) 18
MunI CAATTG 1 cut(s) 590
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeI CATATG 1 cut(s) 476
NdeII GATC 4 cut(s) 298, 539, 628, 679
NlaIII CATG 3 cut(s) 436, 583, 850
NlaIV GGNNCC 4 cut(s) 48, 184, 185, 429
NmuCI GTSAC 2 cut(s) 594, 649
NsiI ATGCAT 1 cut(s) 766
PauI GCGCGC 1 cut(s) 21
PfeI GAWTC 3 cut(s) 459, 688, 795
PkrI GCNGC 2 cut(s) 20, 87
Ple19I CGATCG 1 cut(s) 542
PleI GAGTC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 777
PpuMI RGGWCCY 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 183
PspN4I GGNNCC 4 cut(s) 48, 184, 185, 429
PspPI GGNCC 4 cut(s) 96, 107, 183, 360
PspPPI RGGWCCY 1 cut(s) 183
PsuI RGATCY 1 cut(s) 679
PteI GCGCGC 1 cut(s) 21
PvuI CGATCG 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
RseI CAYNNNNRTG 3 cut(s) 437, 475, 584
SaqAI TTAA 2 cut(s) 173, 521
SatI GCNGC 2 cut(s) 19, 86
Sau3AI GATC 4 cut(s) 298, 539, 628, 679
Sau96I GGNCC 4 cut(s) 96, 107, 183, 360
SchI GAGTC 1 cut(s) 778
SetI ASST 8 cut(s) 20, 109, 122, 241, 362, 380, 709, 782
SfaNI GCATC 1 cut(s) 560
SinI GGWCC 3 cut(s) 107, 183, 360
SmiMI CAYNNNNRTG 3 cut(s) 437, 475, 584
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 4 cut(s) 466, 574, 590, 824
SsiI CCGC 3 cut(s) 67, 86, 161
SspI AATATT 1 cut(s) 673
SspMI CTAG 1 cut(s) 357
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 709
TaqI TCGA 4 cut(s) 254, 301, 483, 538
TasI AATT 4 cut(s) 466, 574, 590, 824
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 3 cut(s) 459, 688, 795
Tru1I TTAA 2 cut(s) 173, 521
Tru9I TTAA 2 cut(s) 173, 521
TscAI CASTG 3 cut(s) 83, 744, 839
TseFI GTSAC 2 cut(s) 594, 649
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 2 cut(s) 594, 649
TspDTI ATGAA 3 cut(s) 240, 459, 596
TspRI CASTG 3 cut(s) 83, 744, 839
VpaK11BI GGWCC 3 cut(s) 107, 183, 360
XapI RAATTY 2 cut(s) 466, 824
XspI CTAG 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.