Rh2DG072600

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006686
Physical Location & Seq
Forward (+)
5573489 .. 5576260
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG072600.1

Sequence Viewer

Length: 792 bp
ATGGCTGTCTTACACAGCTGCGCGCCTTTTCTGCAAAGTCCGAGCATTCCCTTATCCTTGTTTTCCCGCCAGAAACACTGCTCGCCACCGCTTTGGGCCGTCGGAGGTCCATTTCGGAGCTGTTATGGTCAGCCCAAGACAAGTGTATTGGTGGCTAAAGCGGGTCGGGGTCTAAAGTTGGGGACGACCCAGAAAGAGAGTGGAGGAGAAGATGGAGGCTTTGGTGATGAAGGTGAAGATGGGTTTTCGGGTCGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTTGATCGAGACCCTGAGTTTGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAAAAGGAACAAAATATTGCATACAAAATCGGGGTCAGGGGAACCCATGAGAGTGGTATTCAATGACTTTGGTTTCTCAAATTCATACATATGGTTCGAGTTCTACAACATTCCATTGGAACAAGATGTTTCCTTAATCTCGGATACTATTCGATCGTGGCACATTATTGGACGCTTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAATCCCCTTTGGATAAACGACCAAGTTATGATCCCATTCAGGGAGCAAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGATTCAAGACAACTTGGGACGTGTTTGGGTGGATATCGGTACTAAAGAGCCACTGATACTGGATGTTCTAATAAATGCATTGACTCAGATAAGCTCCGAGTATGCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

263

Amino Acids

29.01

Weight (kDa)

5.87

Isoelectric Point (pI)

62.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 143 - 261 2.5e-43 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 67, 89, 161
AclWI GGATC 1 cut(s) 617
AcsI RAATTY 1 cut(s) 460
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 2 cut(s) 346, 632
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 2 cut(s) 442, 686
AjiI CACGTC 1 cut(s) 701
AluBI AGCT 3 cut(s) 18, 120, 774
AluI AGCT 3 cut(s) 18, 120, 774
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 1 cut(s) 617
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 460
ArsI GACNNNNNNTTYG 4 cut(s) 290, 322, 398, 430
AspLEI GCGC 2 cut(s) 23, 25
AspS9I GGNCC 3 cut(s) 96, 107, 354
AsuHPI GGTGA 2 cut(s) 236, 245
AvaII GGWCC 2 cut(s) 107, 354
BbvI GCAGC 1 cut(s) 5
BccI CCATC 3 cut(s) 206, 233, 355
BceAI ACGGC 1 cut(s) 83
BciVI GTATCC 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 1 cut(s) 351
BfuI GTATCC 1 cut(s) 517
BglII AGATCT 1 cut(s) 673
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme18I GGWCC 2 cut(s) 107, 354
BmgBI CACGTC 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 96, 107, 354
BmiI GGNNCC 1 cut(s) 423
BmsI GCATC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 698
BsaI GGTCTC 1 cut(s) 291
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 627, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 632
Bse1I ACTGG 1 cut(s) 744
BseGI GGATG 2 cut(s) 569, 748
BseLI CCNNNNNNNGG 2 cut(s) 346, 632
BseMII CTCAG 2 cut(s) 294, 779
BseNI ACTGG 1 cut(s) 744
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 219, 288, 380
BseXI GCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 536
BshFI GGCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 536
BslFI GGGAC 2 cut(s) 196, 711
BslI CCNNNNNNNGG 2 cut(s) 346, 632
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 2 cut(s) 196, 711
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 1 cut(s) 291
Bsp143I GATC 4 cut(s) 292, 533, 622, 673
BspACI CCGC 3 cut(s) 67, 89, 161
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 295, 778
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 423
BspPI GGATC 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 291
BsrI ACTGG 1 cut(s) 744
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 4 cut(s) 292, 533, 622, 673
BstC8I GCNNGC 2 cut(s) 23, 83
BstDEI CTNAG 2 cut(s) 303, 765
BstF5I GGATG 2 cut(s) 569, 748
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 25
BstKTI GATC 4 cut(s) 295, 536, 625, 676
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 4 cut(s) 292, 533, 622, 673
BstMCI CGRYCG 1 cut(s) 536
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 2 cut(s) 93, 433
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 1 cut(s) 98
BtrI CACGTC 1 cut(s) 701
BtsCI GGATG 2 cut(s) 569, 748
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 76, 731
Cac8I GCNNGC 2 cut(s) 23, 83
CfoI GCGC 2 cut(s) 23, 25
Cfr13I GGNCC 3 cut(s) 96, 107, 354
CseI GACGC 1 cut(s) 561
Csp6I GTAC 1 cut(s) 720
CviAII CATG 2 cut(s) 427, 574
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 2 cut(s) 303, 765
DpnI GATC 4 cut(s) 294, 535, 624, 675
DpnII GATC 4 cut(s) 292, 533, 622, 673
Eco31I GGTCTC 1 cut(s) 291
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 2 cut(s) 107, 354
EcoRV GATATC 1 cut(s) 715
EcoT22I ATGCAT 1 cut(s) 760
FaeI CATG 2 cut(s) 430, 577
FaqI GGGAC 2 cut(s) 196, 711
FatI CATG 2 cut(s) 426, 573
FauI CCCGC 2 cut(s) 74, 154
FauNDI CATATG 1 cut(s) 470
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 2 cut(s) 576, 755
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 1 cut(s) 351
GlaI GCGC 2 cut(s) 22, 24
GluI GCNGC 1 cut(s) 19
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 561
HhaI GCGC 2 cut(s) 23, 25
Hin1II CATG 2 cut(s) 430, 577
Hin6I GCGC 2 cut(s) 21, 23
HinP1I GCGC 2 cut(s) 21, 23
HinfI GANTC 2 cut(s) 682, 763
HphI GGTGA 2 cut(s) 236, 245
Hpy188I TCNGA 7 cut(s) 42, 104, 117, 255, 523, 768, 778
Hpy188III TCNNGA 4 cut(s) 296, 332, 677, 686
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 224, 272, 277
HpyCH4IV ACGT 1 cut(s) 700
HpyCH4V TGCA 5 cut(s) 34, 400, 567, 583, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 303, 765
HpySE526I ACGT 1 cut(s) 700
Hsp92II CATG 2 cut(s) 430, 577
HspAI GCGC 2 cut(s) 21, 23
Kzo9I GATC 4 cut(s) 292, 533, 622, 673
LmnI GCTCC 4 cut(s) 117, 635, 779, 790
LpnPI CCDG 6 cut(s) 83, 203, 315, 402, 617, 725
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 1 cut(s) 554
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 700
MaeIII GTNAC 2 cut(s) 588, 643
MalI GATC 4 cut(s) 294, 535, 624, 675
MboI GATC 4 cut(s) 292, 533, 622, 673
MboII GAAGA 3 cut(s) 221, 248, 391
MfeI CAATTG 1 cut(s) 584
MflI RGATCY 1 cut(s) 673
MluCI AATT 3 cut(s) 460, 568, 584
MlyI GAGTC 1 cut(s) 757
MmeI TCCRAC 2 cut(s) 82, 233
MnlI CCTC 7 cut(s) 98, 197, 209, 266, 358, 363, 369
Mph1103I ATGCAT 1 cut(s) 760
MseI TTAA 2 cut(s) 515, 790
MslI CAYNNNNRTG 3 cut(s) 431, 469, 578
MspA1I CMGCKG 1 cut(s) 18
MunI CAATTG 1 cut(s) 584
Mva1269I GAATGC 1 cut(s) 45
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeI CATATG 1 cut(s) 470
NdeII GATC 4 cut(s) 292, 533, 622, 673
NlaIII CATG 2 cut(s) 430, 577
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 2 cut(s) 588, 643
NsiI ATGCAT 1 cut(s) 760
PauI GCGCGC 1 cut(s) 21
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 682
PkrI GCNGC 1 cut(s) 20
Ple19I CGATCG 1 cut(s) 536
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 3 cut(s) 96, 107, 354
PsuI RGATCY 1 cut(s) 673
PteI GCGCGC 1 cut(s) 21
PvuI CGATCG 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
RseI CAYNNNNRTG 3 cut(s) 431, 469, 578
SaqAI TTAA 2 cut(s) 515, 790
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 4 cut(s) 292, 533, 622, 673
Sau96I GGNCC 3 cut(s) 96, 107, 354
SchI GAGTC 1 cut(s) 757
SetI ASST 8 cut(s) 20, 109, 122, 235, 356, 374, 703, 776
SfaNI GCATC 1 cut(s) 554
SinI GGWCC 2 cut(s) 107, 354
SmiMI CAYNNNNRTG 3 cut(s) 431, 469, 578
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 3 cut(s) 460, 568, 584
SsiI CCGC 3 cut(s) 67, 89, 161
SspI AATATT 2 cut(s) 396, 667
SspMI CTAG 1 cut(s) 351
TaiI ACGT 1 cut(s) 703
TaqI TCGA 3 cut(s) 295, 477, 532
TasI AATT 3 cut(s) 460, 568, 584
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 2 cut(s) 515, 790
Tru9I TTAA 2 cut(s) 515, 790
TscAI CASTG 2 cut(s) 83, 738
TseFI GTSAC 2 cut(s) 588, 643
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 2 cut(s) 588, 643
TspDTI ATGAA 3 cut(s) 243, 453, 590
TspRI CASTG 2 cut(s) 83, 738
VpaK11BI GGWCC 2 cut(s) 107, 354
XapI RAATTY 1 cut(s) 460
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XspI CTAG 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.