Rh2BG074400

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006686
Physical Location & Seq
Forward (+)
5639184 .. 5642014
2831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG074400.1

Sequence Viewer

Length: 789 bp
ATGGCTGTCTTACACAGATGCGCGCCTTTTCTGCAAAGTCCGAGCATTCCCTTATCCTTGTTTTCCCGCCAGAAACACTGCTCGCCACCGTTTTGGGCCGTCGGAGGTCCATTTCGGAGCTGTTATGGTCAGCCCAAGACAAGTGTATTGGTGGCTAAAGCGGGTCGGGGTCTAAAGTTGGGGACCCAGAAAGAGAGTGGAGGAGAAGATGGAGGCTTTGGTGATGAAGGTGAAGATGGGTTTTGGGGTCGGAGTGGGTTTGTGGGGAGAGGAGAAGGGAAGGAGTTTGATCGAGACCCTGAGTTTGCTGAAATAATGGGTGCTTGTCTTGATGACCCACAAAAGGCTAGGTCCAAGATGGAGGAGAGGTTGAGGAAGAAAAGGAACAAGATATTGCATACAAAATCGGGGACAGGGGAACCCATGAGAGTGGTATTCAATGACTTTGGTTTCTCAAATTCATACATATGGTTCGAGTTCTACAACATTCCATTGGAACAAGATGTTTCCTTAATCTCGGATACTATTCGATCGTGGCACATTATTGGACGCTTGGGTGGATGCAATTCCATGAATATGCAATTGTCACAATCCCCTTTGGATAAACGACCAAGTTATGATCCCATTCAGGGAGCAAATGTGACACCAACTACATTTTACAATATTGGAGATCTTGAGATTCAAGACAACTTGGGACGTGTTTGGGTGGATATCGGTACTAAAGAGCCACTGATACTGGATGTTCTGATAAATGCATTGACTCAGATAAGCTCCGAGTATGCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

262

Amino Acids

29.13

Weight (kDa)

6.13

Isoelectric Point (pI)

60.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 142 - 260 2.5e-43 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 67, 161
AclWI GGATC 1 cut(s) 614
AcsI RAATTY 1 cut(s) 457
AfaI GTAC 1 cut(s) 718
AfiI CCNNNNNNNGG 2 cut(s) 343, 629
AflIII ACRYGT 1 cut(s) 697
AgsI TTSAA 2 cut(s) 439, 683
AjiI CACGTC 1 cut(s) 698
AluBI AGCT 2 cut(s) 120, 771
AluI AGCT 2 cut(s) 120, 771
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 614
AoxI GGCC 1 cut(s) 96
ApoI RAATTY 1 cut(s) 457
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspLEI GCGC 2 cut(s) 23, 25
AspS9I GGNCC 4 cut(s) 96, 107, 183, 351
AsuHPI GGTGA 2 cut(s) 233, 242
AvaII GGWCC 3 cut(s) 107, 183, 351
BccI CCATC 3 cut(s) 203, 230, 352
BceAI ACGGC 1 cut(s) 83
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 348
BfuI GTATCC 1 cut(s) 514
BglII AGATCT 1 cut(s) 670
Bme18I GGWCC 3 cut(s) 107, 183, 351
BmgBI CACGTC 1 cut(s) 698
BmgT120I GGNCC 4 cut(s) 96, 107, 183, 351
BmiI GGNNCC 3 cut(s) 184, 185, 420
BmsI GCATC 2 cut(s) 8, 551
BpuEI CTTGAG 1 cut(s) 695
BsaI GGTCTC 1 cut(s) 288
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 624, 654
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 629
Bse1I ACTGG 1 cut(s) 741
BseGI GGATG 2 cut(s) 566, 745
BseLI CCNNNNNNNGG 2 cut(s) 343, 629
BseMII CTCAG 2 cut(s) 291, 776
BseNI ACTGG 1 cut(s) 741
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 216, 285, 377
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 533
BshFI GGCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 533
BslFI GGGAC 3 cut(s) 196, 424, 708
BslI CCNNNNNNNGG 2 cut(s) 343, 629
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 3 cut(s) 196, 424, 708
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 1 cut(s) 288
Bsp143I GATC 4 cut(s) 289, 530, 619, 670
BspACI CCGC 2 cut(s) 67, 161
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 292, 775
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 184, 185, 420
BspPI GGATC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 288
BsrI ACTGG 1 cut(s) 741
BssHII GCGCGC 1 cut(s) 21
BssMI GATC 4 cut(s) 289, 530, 619, 670
Bst4CI ACNGT 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 23, 83
BstDEI CTNAG 2 cut(s) 300, 762
BstF5I GGATG 2 cut(s) 566, 745
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 25
BstKTI GATC 4 cut(s) 292, 533, 622, 673
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 289, 530, 619, 670
BstMCI CGRYCG 1 cut(s) 533
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstUI CGCG 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 670
BstXI CCANNNNNNTGG 2 cut(s) 93, 430
BstYI RGATCY 1 cut(s) 670
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 98
BtrI CACGTC 1 cut(s) 698
BtsCI GGATG 2 cut(s) 566, 745
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 76, 728
Cac8I GCNNGC 2 cut(s) 23, 83
CfoI GCGC 2 cut(s) 23, 25
Cfr13I GGNCC 4 cut(s) 96, 107, 183, 351
CseI GACGC 1 cut(s) 558
Csp6I GTAC 1 cut(s) 717
CviAII CATG 2 cut(s) 424, 571
CviJI RGCY 9 cut(s) 5, 98, 120, 133, 155, 216, 347, 727, 771
CviKI_1 RGCY 9 cut(s) 5, 98, 120, 133, 155, 216, 347, 727, 771
CviQI GTAC 1 cut(s) 717
DdeI CTNAG 2 cut(s) 300, 762
DpnI GATC 4 cut(s) 291, 532, 621, 672
DpnII GATC 4 cut(s) 289, 530, 619, 670
Eco31I GGTCTC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 3 cut(s) 107, 183, 351
EcoO109I RGGNCCY 1 cut(s) 183
EcoRV GATATC 1 cut(s) 712
EcoT22I ATGCAT 1 cut(s) 757
FaeI CATG 2 cut(s) 427, 574
FaqI GGGAC 3 cut(s) 196, 424, 708
FatI CATG 2 cut(s) 423, 570
FauI CCCGC 2 cut(s) 74, 154
FauNDI CATATG 1 cut(s) 467
FokI GGATG 2 cut(s) 573, 752
FspBI CTAG 1 cut(s) 348
GlaI GCGC 2 cut(s) 22, 24
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 558
HhaI GCGC 2 cut(s) 23, 25
Hin1II CATG 2 cut(s) 427, 574
Hin6I GCGC 2 cut(s) 21, 23
HinP1I GCGC 2 cut(s) 21, 23
HinfI GANTC 2 cut(s) 679, 760
HphI GGTGA 2 cut(s) 233, 242
Hpy188I TCNGA 8 cut(s) 42, 104, 117, 252, 520, 747, 765, 775
Hpy188III TCNNGA 4 cut(s) 293, 329, 674, 683
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 221, 269, 274
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 5 cut(s) 34, 397, 564, 580, 755
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 300, 762
HpySE526I ACGT 1 cut(s) 697
Hsp92II CATG 2 cut(s) 427, 574
HspAI GCGC 2 cut(s) 21, 23
KflI GGGWCCC 1 cut(s) 183
Kzo9I GATC 4 cut(s) 289, 530, 619, 670
LmnI GCTCC 4 cut(s) 117, 632, 776, 787
LpnPI CCDG 6 cut(s) 83, 200, 312, 399, 614, 722
LweI GCATC 2 cut(s) 8, 551
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 2 cut(s) 585, 640
MalI GATC 4 cut(s) 291, 532, 621, 672
MboI GATC 4 cut(s) 289, 530, 619, 670
MboII GAAGA 3 cut(s) 218, 245, 388
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 1 cut(s) 670
MluCI AATT 3 cut(s) 457, 565, 581
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 2 cut(s) 82, 230
MnlI CCTC 7 cut(s) 98, 194, 206, 263, 355, 360, 366
Mph1103I ATGCAT 1 cut(s) 757
MseI TTAA 2 cut(s) 512, 787
MslI CAYNNNNRTG 3 cut(s) 428, 466, 575
MunI CAATTG 1 cut(s) 581
Mva1269I GAATGC 1 cut(s) 45
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeI CATATG 1 cut(s) 467
NdeII GATC 4 cut(s) 289, 530, 619, 670
NlaIII CATG 2 cut(s) 427, 574
NlaIV GGNNCC 3 cut(s) 184, 185, 420
NmuCI GTSAC 2 cut(s) 585, 640
NsiI ATGCAT 1 cut(s) 757
PauI GCGCGC 1 cut(s) 21
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 679
Ple19I CGATCG 1 cut(s) 533
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PpuMI RGGWCCY 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 184, 185, 420
PspPI GGNCC 4 cut(s) 96, 107, 183, 351
PspPPI RGGWCCY 1 cut(s) 183
PsuI RGATCY 1 cut(s) 670
PteI GCGCGC 1 cut(s) 21
PvuI CGATCG 1 cut(s) 533
RsaI GTAC 1 cut(s) 718
RsaNI GTAC 1 cut(s) 717
RseI CAYNNNNRTG 3 cut(s) 428, 466, 575
SaqAI TTAA 2 cut(s) 512, 787
Sau3AI GATC 4 cut(s) 289, 530, 619, 670
Sau96I GGNCC 4 cut(s) 96, 107, 183, 351
SchI GAGTC 1 cut(s) 754
SetI ASST 7 cut(s) 109, 122, 232, 353, 371, 700, 773
SfaNI GCATC 2 cut(s) 8, 551
SinI GGWCC 3 cut(s) 107, 183, 351
SmiMI CAYNNNNRTG 3 cut(s) 428, 466, 575
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 3 cut(s) 457, 565, 581
SsiI CCGC 2 cut(s) 67, 161
SspI AATATT 1 cut(s) 664
SspMI CTAG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 90
TaiI ACGT 1 cut(s) 700
TaqI TCGA 3 cut(s) 292, 474, 529
TasI AATT 3 cut(s) 457, 565, 581
TfiI GAWTC 1 cut(s) 679
Tru1I TTAA 2 cut(s) 512, 787
Tru9I TTAA 2 cut(s) 512, 787
TscAI CASTG 2 cut(s) 83, 735
TseFI GTSAC 2 cut(s) 585, 640
Tsp45I GTSAC 2 cut(s) 585, 640
TspDTI ATGAA 3 cut(s) 240, 450, 587
TspRI CASTG 2 cut(s) 83, 735
VpaK11BI GGWCC 3 cut(s) 107, 183, 351
XapI RAATTY 1 cut(s) 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.