AT4G32270

ATP synthesis coupled proton transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009777
Physical Location & Seq
Reverse (-)
15574869 .. 15576540
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32270.1

Sequence Viewer

Length: 720 bp
ATGGACGTTCCACCACGCCGCTCTTTGGCTGCGTCTCTCTCGCCTCTCCGGCTAATCGATGGACTCCCTCGTCGGAGGAGTTTCAATTATAATCAGATGCCTGAAGAGCCCATCAAACTCACTGTTCTCAAGCTTGATGGATCTTCCTTTGGTATTCAAGTGTTGAAGACAGCGACAGTAGGGGAGCTCAAGATGGCAGTAGAAGCTGCGTTTTCTCACTTGCCCATCTCAGGCCCTGGCAAGATCTCATGGCCGCATGTTTGGGGACAATTCTGCTTATCTTATGAAGATAAAAGATTGATTAATGAGTCTGAGTACCTCACTGAATTTGGCATTAAAGACGGCGATCAGCTTCGGTTCATCCGCCATATCTCAAACTACTGCATGTTAATGGTGAAGCATAAGAGCAAGACACCCCGTGTTTCTTCTTTCAAACAACTCAAACTGTTCTCAACCACACCGGAAACCCGGAAAAAGAACGTAATAAGGGAAGTTGAAGAAGACGGTGTTGTTGACTCCATCCCTCGGAGCCAACCAAGCTTTCTAGCAACTGTATTGGGAGGTTTGCTCTCTTACAAGTCGACGCCAAGTCAACGAGGTACTAAATACAGAAACGTGACTGCAAGTAGAGTTTTTAACAAACTCATTGCAAGGTTTCGTTTCAAGTGTAATTCCGAGAAGGATGTGTGGAACAGAAAAAAACTCATATCCGAAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

239

Amino Acids

27.27

Weight (kDa)

9.97

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 38 - 124 4e-24 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 90
AasI GACNNNNNNGTC 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 21
AccI GTMKAC 1 cut(s) 581
AciI CCGC 3 cut(s) 19, 254, 364
AclWI GGATC 1 cut(s) 148
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 584
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 2 cut(s) 317, 601
AfiI CCNNNNNNNGG 3 cut(s) 25, 230, 525
AgsI TTSAA 6 cut(s) 85, 158, 166, 433, 497, 664
AhdI GACNNNNNGTC 1 cut(s) 588
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 5 cut(s) 133, 187, 206, 352, 540
AluI AGCT 5 cut(s) 133, 187, 206, 352, 540
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 2 cut(s) 232, 251
ApeKI GCWGC 2 cut(s) 29, 206
ApoI RAATTY 1 cut(s) 326
AseI ATTAAT 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 406
BanII GRGCYC 2 cut(s) 111, 189
BbsI GAAGAC 2 cut(s) 173, 507
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 16, 193
BccI CCATC 6 cut(s) 53, 119, 131, 187, 233, 527
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 1 cut(s) 545
BglI GCCNNNNNGGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 243
BisI GCNGC 4 cut(s) 19, 30, 207, 254
BlsI GCNGC 4 cut(s) 20, 31, 208, 255
Bme1390I CCNGG 2 cut(s) 237, 469
BmeRI GACNNNNNGTC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 530
BmrFI CCNGG 2 cut(s) 237, 469
BmsI GCATC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 173, 507
BplI GAGNNNNNCTC 2 cut(s) 552, 584
BpuEI CTTGAG 2 cut(s) 113, 173
BpuMI CCSGG 1 cut(s) 469
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 584
BsaJI CCNNGG 2 cut(s) 235, 524
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 230, 525
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 235, 524
BseGI GGATG 3 cut(s) 360, 519, 688
BseLI CCNNNNNNNGG 3 cut(s) 25, 230, 525
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 243, 303
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 2 cut(s) 16, 193
BshFI GGCC 2 cut(s) 234, 253
BshVI ATCGAT 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 3 cut(s) 49, 461, 469
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 3 cut(s) 25, 230, 525
BsmAI GTCTC 1 cut(s) 39
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 2 cut(s) 234, 253
Bsp1286I GDGCHC 2 cut(s) 111, 189
Bsp143I GATC 3 cut(s) 140, 243, 346
BspACI CCGC 3 cut(s) 19, 254, 364
BspANI GGCC 2 cut(s) 234, 253
BspCNI CTCAG 2 cut(s) 242, 304
BspDI ATCGAT 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 530
BspPI GGATC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 99
BsrBI CCGCTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 645
BssECI CCNNGG 2 cut(s) 235, 524
BssMI GATC 3 cut(s) 140, 243, 346
BssNI GRCGYC 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 5 cut(s) 124, 178, 447, 506, 553
Bst6I CTCTTC 1 cut(s) 99
BstACI GRCGYC 1 cut(s) 584
BstDEI CTNAG 2 cut(s) 229, 312
BstF5I GGATG 3 cut(s) 360, 519, 688
BstKTI GATC 3 cut(s) 143, 246, 349
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 3 cut(s) 140, 243, 346
BstMWI GCNNNNNNNGC 4 cut(s) 49, 106, 203, 537
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 2 cut(s) 260, 388
BstSCI CCNGG 2 cut(s) 235, 467
BstV1I GCAGC 2 cut(s) 16, 193
BstV2I GAAGAC 2 cut(s) 173, 507
BstX2I RGATCY 2 cut(s) 140, 243
BstYI RGATCY 2 cut(s) 140, 243
Bsu15I ATCGAT 1 cut(s) 57
BsuRI GGCC 2 cut(s) 234, 253
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 3 cut(s) 360, 519, 688
BtsIMutI CAGTG 2 cut(s) 120, 321
CaiI CAGNNNCTG 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 2 cut(s) 21, 592
Csp6I GTAC 2 cut(s) 316, 600
CviAII CATG 3 cut(s) 249, 257, 385
CviQI GTAC 2 cut(s) 316, 600
DdeI CTNAG 2 cut(s) 229, 312
DpnI GATC 3 cut(s) 142, 245, 348
DpnII GATC 3 cut(s) 140, 243, 346
DraIII CACNNNGTG 1 cut(s) 419
DrdI GACNNNNNNGTC 1 cut(s) 69
DriI GACNNNNNGTC 1 cut(s) 588
DseDI GACNNNNNNGTC 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 99
Eam1105I GACNNNNNGTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 2 cut(s) 111, 189
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 235
EcoT38I GRGCYC 2 cut(s) 111, 189
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 3 cut(s) 252, 260, 388
FaiI YATR 8 cut(s) 90, 250, 258, 285, 369, 386, 402, 707
FaqI GGGAC 1 cut(s) 279
FatI CATG 3 cut(s) 248, 256, 384
FblI GTMKAC 1 cut(s) 581
Fnu4HI GCNGC 4 cut(s) 19, 30, 207, 254
FokI GGATG 3 cut(s) 347, 506, 695
FriOI GRGCYC 2 cut(s) 111, 189
Fsp4HI GCNGC 4 cut(s) 19, 30, 207, 254
FspBI CTAG 1 cut(s) 545
GluI GCNGC 4 cut(s) 19, 30, 207, 254
HaeIII GGCC 2 cut(s) 234, 253
HapII CCGG 3 cut(s) 49, 461, 469
HgaI GACGC 2 cut(s) 21, 592
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 3 cut(s) 252, 260, 388
HincII GTYRAC 3 cut(s) 514, 582, 593
HindII GTYRAC 3 cut(s) 514, 582, 593
HindIII AAGCTT 2 cut(s) 131, 538
HinfI GANTC 3 cut(s) 63, 308, 515
HpaII CCGG 3 cut(s) 49, 461, 469
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 3 cut(s) 514, 582, 593
Hpy188I TCNGA 6 cut(s) 75, 96, 313, 528, 676, 712
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 3 cut(s) 514, 582, 593
Hpy99I CGWCG 2 cut(s) 75, 586
HpyAV CCTTC 1 cut(s) 673
HpyCH4III ACNGT 5 cut(s) 124, 178, 447, 506, 553
HpyCH4IV ACGT 3 cut(s) 6, 480, 615
HpyCH4V TGCA 3 cut(s) 384, 623, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 106, 203, 537
HpyF3I CTNAG 2 cut(s) 229, 312
HpySE526I ACGT 3 cut(s) 6, 480, 615
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 3 cut(s) 252, 260, 388
Kzo9I GATC 3 cut(s) 140, 243, 346
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 2 cut(s) 184, 528
LpnPI CCDG 7 cut(s) 62, 114, 216, 222, 249, 474, 482
Lsp1109I GCAGC 2 cut(s) 16, 193
LweI GCATC 1 cut(s) 87
MaeI CTAG 1 cut(s) 545
MaeII ACGT 3 cut(s) 6, 480, 615
MaeIII GTNAC 1 cut(s) 616
MalI GATC 3 cut(s) 142, 245, 348
MbiI CCGCTC 1 cut(s) 21
MboI GATC 3 cut(s) 140, 243, 346
MboII GAAGA 7 cut(s) 116, 135, 178, 299, 417, 509, 512
MflI RGATCY 2 cut(s) 140, 243
MhlI GDGCHC 2 cut(s) 111, 189
MluCI AATT 4 cut(s) 85, 269, 326, 670
MlyI GAGTC 3 cut(s) 57, 317, 509
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 7 cut(s) 54, 69, 78, 329, 534, 554, 590
MseI TTAA 4 cut(s) 303, 336, 389, 636
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 3 cut(s) 49, 461, 469
MspR9I CCNGG 2 cut(s) 237, 469
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 4 cut(s) 49, 106, 203, 537
NciI CCSGG 1 cut(s) 469
NdeII GATC 3 cut(s) 140, 243, 346
NlaIII CATG 3 cut(s) 252, 260, 388
NlaIV GGNNCC 1 cut(s) 530
NmuCI GTSAC 1 cut(s) 616
NspI RCATGY 2 cut(s) 260, 388
PciSI GCTCTTC 1 cut(s) 99
PkrI GCNGC 4 cut(s) 20, 31, 208, 255
PleI GAGTC 3 cut(s) 57, 316, 509
PpsI GAGTC 3 cut(s) 57, 316, 509
PshBI ATTAAT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 530
PspPI GGNCC 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 2 cut(s) 140, 243
RsaI GTAC 2 cut(s) 317, 601
RsaNI GTAC 2 cut(s) 316, 600
RseI CAYNNNNRTG 1 cut(s) 389
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 1 cut(s) 580
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 4 cut(s) 303, 336, 389, 636
SatI GCNGC 4 cut(s) 19, 30, 207, 254
Sau3AI GATC 3 cut(s) 140, 243, 346
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 3 cut(s) 57, 317, 509
ScrFI CCNGG 2 cut(s) 237, 469
SduI GDGCHC 2 cut(s) 111, 189
SfaNI GCATC 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 2 cut(s) 128, 188
SmoI CTYRAG 2 cut(s) 128, 188
Sse9I AATT 4 cut(s) 85, 269, 326, 670
SsiI CCGC 3 cut(s) 19, 254, 364
SspMI CTAG 1 cut(s) 545
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 235, 467
TaaI ACNGT 5 cut(s) 124, 178, 447, 506, 553
TaiI ACGT 3 cut(s) 9, 483, 618
TaqI TCGA 2 cut(s) 57, 581
TasI AATT 4 cut(s) 85, 269, 326, 670
TauI GCSGC 2 cut(s) 21, 256
Tru1I TTAA 4 cut(s) 303, 336, 389, 636
Tru9I TTAA 4 cut(s) 303, 336, 389, 636
TscAI CASTG 2 cut(s) 127, 328
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 2 cut(s) 29, 206
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 2 cut(s) 300, 349
TspRI CASTG 2 cut(s) 127, 328
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 1 cut(s) 326
XceI RCATGY 2 cut(s) 260, 388
XmiI GTMKAC 1 cut(s) 581
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.