MD15G1126000.v1.1

ATP synthase B/B' CF(0)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009777
Physical Location & Seq
Forward (+)
9165881 .. 9168252
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1126000.v1.1.491

Sequence Viewer

Length: 858 bp
ATGTCGTATAGCGCGCTGAGAATTGACGATGATGATTTTATCCCAGTTGGGCGGTCGTCGATCGAACGGCCTCGCCTCTCCAATTCTCTGCCGTTTGTGCCGTTGCTACTCGTCGATGGCTTCTCCCGCAAGAGCTTGTCGTACTCCGCGCTTCCTCAGGAGCCTCTCCGGCTCTCCGTTCTCAAATTGGATGGCTCTTGCTTCGATATTCAAGTTTCCATGACAGCCACTATTTTGGAATTAAAGCAGGCAGTCGAGGCCGTTTTCAGTCACATGCCACAGAAGGGACCAGGAAAAATTTCATGGCCACATGTGTGGGGTCATTTCTGCTTGTGCTACGATAATCAGAAGCTACTTGTTGAAACTGATCATATCAAACTTTATGGCATCAAAGACGGTGATCAGCTGCATTTTGTCCGGCATGTTTCAATCAACTACAGCATGACAAAGAGCCAATCGTCAAAGAAAGGGTTCCTTGCTTTAAAACAAAACACAACGTCAATGTTGCGATCATTGAGCTTTCAAGAGGAAGACCCGACTGACATTGAGAAAGAAGAGAATGGCAAAGACGTAGAACAGAATGACGAGGTATGTTATGATTCTGATCACATCAAGAATCCAAAGTACCCGCATTGCGATGACGAAGTTTCTATCGAACACAATGAGAGCAGGCTGCCCTTCTTATTGGGAGAATGGTTTCCATACTCCAAACTGTCAGCTGTAGGAACAACATCTAAAAGAACTTGGGCGCCAAGCATAGCCAGTGGGTTGTTGCTTGGGTTTAGGAAGATATTTGTGCTTTGTTTCGAGAAACGCGATAAACGGTCTAGTCGAAGGGATACTTGGAGAACAGATTAG

Protein Analysis

286

Amino Acids

32.6

Weight (kDa)

6.51

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 56 - 142 3.5e-26 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 748
AccII CGCG 3 cut(s) 14, 149, 816
AciI CCGC 4 cut(s) 52, 127, 147, 629
AcoI YGGCCR 1 cut(s) 305
AcsI RAATTY 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 749
AfaI GTAC 2 cut(s) 143, 626
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 4 cut(s) 212, 362, 429, 524
AjnI CCWGG 1 cut(s) 289
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 5 cut(s) 135, 352, 406, 519, 719
AluI AGCT 5 cut(s) 135, 352, 406, 519, 719
AoxI GGCC 3 cut(s) 68, 258, 305
ApeKI GCWGC 2 cut(s) 406, 673
ApoI RAATTY 1 cut(s) 297
Asp700I GAANNNNTTC 3 cut(s) 298, 470, 696
AspLEI GCGC 4 cut(s) 14, 16, 151, 751
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 410
AvaII GGWCC 1 cut(s) 287
AxyI CCTNAGG 1 cut(s) 156
BalI TGGCCA 1 cut(s) 307
BanI GGYRCC 1 cut(s) 748
BbsI GAAGAC 1 cut(s) 537
BbvI GCAGC 2 cut(s) 393, 660
BccI CCATC 2 cut(s) 110, 185
BceAI ACGGC 4 cut(s) 76, 83, 85, 245
BciT130I CCWGG 1 cut(s) 291
BciVI GTATCC 1 cut(s) 832
BclI TGATCA 3 cut(s) 367, 400, 604
BfaI CTAG 1 cut(s) 828
BfmI CTRYAG 2 cut(s) 436, 720
BfoI RGCGCY 1 cut(s) 752
BfuI GTATCC 1 cut(s) 832
BglI GCCNNNNNGGC 1 cut(s) 169
BisI GCNGC 2 cut(s) 407, 674
BlsI GCNGC 2 cut(s) 408, 675
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 4 cut(s) 162, 288, 473, 750
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 38
BmsI GCATC 1 cut(s) 396
BmuI ACTGGG 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 537
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 749
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 2 cut(s) 44, 762
Bse21I CCTNAGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 631
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 2 cut(s) 8, 170
BseNI ACTGG 2 cut(s) 44, 762
BsePI GCGCGC 1 cut(s) 12
BseXI GCAGC 2 cut(s) 393, 660
Bsh1236I CGCG 3 cut(s) 14, 149, 816
Bsh1285I CGRYCG 2 cut(s) 56, 63
BshFI GGCC 3 cut(s) 70, 260, 307
BshNI GGYRCC 1 cut(s) 748
BsiEI CGRYCG 2 cut(s) 56, 63
BsiSI CCGG 2 cut(s) 169, 418
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 300
BsnI GGCC 3 cut(s) 70, 260, 307
Bsp143I GATC 5 cut(s) 60, 367, 400, 509, 604
BspACI CCGC 4 cut(s) 52, 127, 147, 629
BspANI GGCC 3 cut(s) 70, 260, 307
BspCNI CTCAG 2 cut(s) 9, 169
BspFNI CGCG 3 cut(s) 14, 149, 816
BspLI GGNNCC 4 cut(s) 162, 288, 473, 750
BspT107I GGYRCC 1 cut(s) 748
BsrDI GCAATG 1 cut(s) 631
BsrI ACTGG 2 cut(s) 44, 762
BssHII GCGCGC 1 cut(s) 12
BssMI GATC 5 cut(s) 60, 367, 400, 509, 604
BssNI GRCGYC 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 398, 714, 825
Bst6I CTCTTC 1 cut(s) 549
BstACI GRCGYC 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 14, 249, 671
BstDEI CTNAG 2 cut(s) 17, 156
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 3 cut(s) 14, 149, 816
BstH2I RGCGCY 1 cut(s) 752
BstHHI GCGC 4 cut(s) 14, 16, 151, 751
BstKTI GATC 5 cut(s) 63, 370, 403, 512, 607
BstMBI GATC 5 cut(s) 60, 367, 400, 509, 604
BstMCI CGRYCG 2 cut(s) 56, 63
BstMWI GCNNNNNNNGC 4 cut(s) 97, 126, 169, 257
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 3 cut(s) 277, 314, 425
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 2 cut(s) 436, 720
BstUI CGCG 3 cut(s) 14, 149, 816
BstV1I GCAGC 2 cut(s) 393, 660
BstV2I GAAGAC 1 cut(s) 537
BstXI CCANNNNNNTGG 2 cut(s) 235, 315
Bsu36I CCTNAGG 1 cut(s) 156
BsuI GTATCC 1 cut(s) 832
BsuRI GGCC 3 cut(s) 70, 260, 307
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 769
Cac8I GCNNGC 3 cut(s) 14, 249, 671
CfoI GCGC 4 cut(s) 14, 16, 151, 751
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 2 cut(s) 142, 625
CviAII CATG 6 cut(s) 220, 274, 303, 311, 422, 442
CviQI GTAC 2 cut(s) 142, 625
DdeI CTNAG 2 cut(s) 17, 156
DinI GGCGCC 1 cut(s) 750
DpnI GATC 5 cut(s) 62, 369, 402, 511, 606
DpnII GATC 5 cut(s) 60, 367, 400, 509, 604
DraI TTTAAA 1 cut(s) 483
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 287
Eco81I CCTNAGG 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 289
EgeI GGCGCC 1 cut(s) 750
EheI GGCGCC 1 cut(s) 750
FaeI CATG 6 cut(s) 223, 277, 306, 314, 425, 445
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 826, 858
FaqI GGGAC 1 cut(s) 300
FatI CATG 6 cut(s) 219, 273, 302, 310, 421, 441
FauI CCCGC 2 cut(s) 134, 636
FbaI TGATCA 3 cut(s) 367, 400, 604
Fnu4HI GCNGC 2 cut(s) 407, 674
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 407, 674
FspBI CTAG 1 cut(s) 828
GlaI GCGC 4 cut(s) 13, 15, 150, 750
GluI GCNGC 2 cut(s) 407, 674
HaeII RGCGCY 1 cut(s) 752
HaeIII GGCC 3 cut(s) 70, 260, 307
HapII CCGG 2 cut(s) 169, 418
HhaI GCGC 4 cut(s) 14, 16, 151, 751
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 6 cut(s) 223, 277, 306, 314, 425, 445
Hin6I GCGC 4 cut(s) 12, 14, 149, 749
HinP1I GCGC 4 cut(s) 12, 14, 149, 749
HinfI GANTC 2 cut(s) 599, 616
HpaII CCGG 2 cut(s) 169, 418
HphI GGTGA 1 cut(s) 410
Hpy188I TCNGA 2 cut(s) 348, 604
Hpy188III TCNNGA 4 cut(s) 158, 524, 613, 808
Hpy99I CGWCG 2 cut(s) 61, 116
HpyAV CCTTC 3 cut(s) 277, 688, 828
HpyCH4III ACNGT 3 cut(s) 398, 714, 825
HpyCH4IV ACGT 2 cut(s) 497, 570
HpyCH4V TGCA 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 126, 169, 257
HpyF3I CTNAG 2 cut(s) 17, 156
HpySE526I ACGT 2 cut(s) 497, 570
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 6 cut(s) 223, 277, 306, 314, 425, 445
HspAI GCGC 4 cut(s) 12, 14, 149, 749
KasI GGCGCC 1 cut(s) 748
Ksp22I TGATCA 3 cut(s) 367, 400, 604
Kzo9I GATC 5 cut(s) 60, 367, 400, 509, 604
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 9 cut(s) 57, 143, 182, 233, 276, 303, 431, 655, 775
Lsp1109I GCAGC 2 cut(s) 393, 660
LweI GCATC 1 cut(s) 396
MaeI CTAG 1 cut(s) 828
MaeII ACGT 2 cut(s) 497, 570
MaeIII GTNAC 1 cut(s) 269
MalI GATC 5 cut(s) 62, 369, 402, 511, 606
MboI GATC 5 cut(s) 60, 367, 400, 509, 604
MboII GAAGA 3 cut(s) 542, 566, 799
MlsI TGGCCA 1 cut(s) 307
MluCI AATT 5 cut(s) 21, 82, 185, 239, 297
MluNI TGGCCA 1 cut(s) 307
Mly113I GGCGCC 1 cut(s) 749
MnlI CCTC 7 cut(s) 81, 86, 165, 174, 250, 520, 580
Mox20I TGGCCA 1 cut(s) 307
MroXI GAANNNNTTC 3 cut(s) 298, 470, 696
MscI TGGCCA 1 cut(s) 307
MseI TTAA 2 cut(s) 242, 482
MslI CAYNNNNRTG 2 cut(s) 313, 636
Msp20I TGGCCA 1 cut(s) 307
MspA1I CMGCKG 2 cut(s) 406, 719
MspI CCGG 2 cut(s) 169, 418
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 3 cut(s) 14, 149, 816
MwoI GCNNNNNNNGC 4 cut(s) 97, 126, 169, 257
NarI GGCGCC 1 cut(s) 749
NdeII GATC 5 cut(s) 60, 367, 400, 509, 604
NlaIII CATG 6 cut(s) 223, 277, 306, 314, 425, 445
NlaIV GGNNCC 4 cut(s) 162, 288, 473, 750
NmuCI GTSAC 1 cut(s) 269
NspI RCATGY 3 cut(s) 277, 314, 425
OliI CACNNNNGTG 1 cut(s) 313
PauI GCGCGC 1 cut(s) 12
PciI ACATGT 1 cut(s) 310
PcsI WCGNNNNNNNCGW 2 cut(s) 813, 829
PdmI GAANNNNTTC 3 cut(s) 298, 470, 696
PfeI GAWTC 2 cut(s) 599, 616
PkrI GCNGC 2 cut(s) 408, 675
Ple19I CGATCG 1 cut(s) 63
PluTI GGCGCC 1 cut(s) 752
PscI ACATGT 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 4 cut(s) 162, 288, 473, 750
PspPI GGNCC 1 cut(s) 287
PteI GCGCGC 1 cut(s) 12
PvuI CGATCG 1 cut(s) 63
PvuII CAGCTG 2 cut(s) 406, 719
RsaI GTAC 2 cut(s) 143, 626
RsaNI GTAC 2 cut(s) 142, 625
RseI CAYNNNNRTG 2 cut(s) 313, 636
SaqAI TTAA 2 cut(s) 242, 482
SatI GCNGC 2 cut(s) 407, 674
Sau3AI GATC 5 cut(s) 60, 367, 400, 509, 604
Sau96I GGNCC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 291
SetI ASST 8 cut(s) 137, 354, 408, 500, 521, 573, 591, 721
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 2 cut(s) 436, 720
SfoI GGCGCC 1 cut(s) 750
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 2 cut(s) 313, 636
Sse9I AATT 5 cut(s) 21, 82, 185, 239, 297
SsiI CCGC 4 cut(s) 52, 127, 147, 629
SspDI GGCGCC 1 cut(s) 748
SspMI CTAG 1 cut(s) 828
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 398, 714, 825
TaiI ACGT 2 cut(s) 500, 573
TaqI TCGA 8 cut(s) 59, 63, 114, 204, 255, 654, 807, 832
TasI AATT 5 cut(s) 21, 82, 185, 239, 297
TfiI GAWTC 2 cut(s) 599, 616
Tru1I TTAA 2 cut(s) 242, 482
Tru9I TTAA 2 cut(s) 242, 482
TscAI CASTG 1 cut(s) 769
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 2 cut(s) 406, 673
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 291
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 1 cut(s) 769
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 297
XceI RCATGY 3 cut(s) 277, 314, 425
XmnI GAANNNNTTC 3 cut(s) 298, 470, 696
XspI CTAG 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.