AT5G25340

ATP synthesis coupled proton transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009777
Physical Location & Seq
Forward (+)
8793018 .. 8794784
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25340.1

Sequence Viewer

Length: 627 bp
ATGGGAAGCTTTCATCGCCGGACTTTTTCTTATGATAAACTTCCGAACGAACCCATTAGGCTCTCCGTTCTCAAACTAGATGGCTCTTCCTTTGATGTTTACGTATTGACCTCGGCCACCGTTGGGGATCTCAAGGTTGCTATAGAAACTGCCTTCAGCCATGTCCCCAAGAAGGGACCCTCCAAGATCTCATGGTCACACGTATGGGGGCATTTTTGTTTGTGTTTCGGAGGTCAGAAGTTAATTACCGATACGGATTGCATCGGAAATTATGGGATGAAGGACGGTGATGAGGTTAGGTTCAAAAACCATGTATCCGGCAATGCGGTATTGAGCAAGGGATATTCTAGGAAATCCAAACAAAAGAATTTAGAGAGGGTATTGCCAAAGGATGGAGATGAAGTATTGAATAGGATTGAAGAGATTGATGACTCTTGGGATGATCTTGAGAGAGGAGGTTTAGTGATGTACAAAGATGATGACATGGAAACTTCTTCAAATGAAAGCAACAGGTCTTGTCTATCCACTGTACATGGTTGCTGCTTTGCTTTCGGTTTAAAAGAGCTTCTTGGGTTTGGTAATGACAGAGCTTATTACTCTTTGAGAGATACTTGGAGAGACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.45

Weight (kDa)

5.88

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 19 - 105 1.6e-24 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 392
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 135
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 139
AfaI GTAC 2 cut(s) 470, 531
AfiI CCNNNNNNNGG 4 cut(s) 123, 172, 173, 392
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 4 cut(s) 304, 409, 419, 498
AluBI AGCT 3 cut(s) 9, 565, 590
AluI AGCT 3 cut(s) 9, 565, 590
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 1 cut(s) 135
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 527
BccI CCATC 2 cut(s) 74, 386
BciVI GTATCC 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 2 cut(s) 77, 348
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 325
BglII AGATCT 1 cut(s) 186
BisI GCNGC 1 cut(s) 541
BlsI GCNGC 1 cut(s) 542
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 177, 178
BmsI GCATC 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 116, 467
BsaAI YACGTR 2 cut(s) 103, 202
BsaJI CCNNGG 1 cut(s) 111
BsaXI ACNNNNNCTCC 4 cut(s) 387, 417, 447, 477
Bsc4I CCNNNNNNNGG 4 cut(s) 123, 172, 173, 392
Bse3DI GCAATG 1 cut(s) 328
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 3 cut(s) 282, 397, 445
BseLI CCNNNNNNNGG 4 cut(s) 123, 172, 173, 392
BseMI GCAATG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 468
BseXI GCAGC 1 cut(s) 527
BshFI GGCC 1 cut(s) 116
BsiSI CCGG 2 cut(s) 19, 318
BslFI GGGAC 2 cut(s) 149, 189
BslI CCNNNNNNNGG 4 cut(s) 123, 172, 173, 392
BsmAI GTCTC 1 cut(s) 612
BsmBI CGTCTC 1 cut(s) 612
BsmFI GGGAC 2 cut(s) 149, 189
BsnI GGCC 1 cut(s) 116
Bsp1407I TGTACA 2 cut(s) 468, 529
Bsp143I GATC 3 cut(s) 127, 186, 442
BspACI CCGC 1 cut(s) 326
BspANI GGCC 1 cut(s) 116
BspLI GGNNCC 2 cut(s) 177, 178
BspPI GGATC 1 cut(s) 135
BspQI GCTCTTC 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 328
BsrGI TGTACA 2 cut(s) 468, 529
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 127, 186, 442
Bst4CI ACNGT 3 cut(s) 121, 287, 529
Bst6I CTCTTC 2 cut(s) 91, 414
BstAUI TGTACA 2 cut(s) 468, 529
BstBAI YACGTR 2 cut(s) 103, 202
BstF5I GGATG 3 cut(s) 282, 397, 445
BstKTI GATC 3 cut(s) 130, 189, 445
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 3 cut(s) 127, 186, 442
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 141
BstSNI TACGTA 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 527
BstX2I RGATCY 2 cut(s) 127, 186
BstYI RGATCY 2 cut(s) 127, 186
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 1 cut(s) 116
BtsCI GGATG 3 cut(s) 282, 397, 445
BtsIMutI CAGTG 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 469, 530
CviAII CATG 5 cut(s) 161, 192, 311, 484, 533
CviJI RGCY 7 cut(s) 9, 61, 84, 116, 159, 565, 590
CviKI_1 RGCY 7 cut(s) 9, 61, 84, 116, 159, 565, 590
CviQI GTAC 2 cut(s) 469, 530
DpnI GATC 3 cut(s) 129, 188, 444
DpnII GATC 3 cut(s) 127, 186, 442
DraI TTTAAA 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 2 cut(s) 91, 414
EarI CTCTTC 2 cut(s) 91, 414
Eco105I TACGTA 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 612
FaeI CATG 5 cut(s) 164, 195, 314, 487, 536
FaiI YATR 9 cut(s) 33, 143, 162, 193, 205, 273, 312, 485, 534
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FaqI GGGAC 2 cut(s) 149, 189
FatI CATG 5 cut(s) 160, 191, 310, 483, 532
Fnu4HI GCNGC 1 cut(s) 541
FokI GGATG 3 cut(s) 289, 404, 452
Fsp4HI GCNGC 1 cut(s) 541
FspBI CTAG 2 cut(s) 77, 348
GluI GCNGC 1 cut(s) 541
HaeIII GGCC 1 cut(s) 116
HapII CCGG 2 cut(s) 19, 318
Hin1II CATG 5 cut(s) 164, 195, 314, 487, 536
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 1 cut(s) 431
HpaII CCGG 2 cut(s) 19, 318
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 4 cut(s) 45, 230, 237, 266
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 3 cut(s) 163, 166, 274
HpyCH4III ACNGT 3 cut(s) 121, 287, 529
HpyCH4IV ACGT 2 cut(s) 102, 201
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
HpySE526I ACGT 2 cut(s) 102, 201
Hsp92II CATG 5 cut(s) 164, 195, 314, 487, 536
KflI GGGWCCC 1 cut(s) 176
Kzo9I GATC 3 cut(s) 127, 186, 442
LguI GCTCTTC 1 cut(s) 91
LpnPI CCDG 3 cut(s) 32, 331, 496
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 270
MaeI CTAG 2 cut(s) 77, 348
MaeII ACGT 2 cut(s) 102, 201
MaeIII GTNAC 1 cut(s) 195
MalI GATC 3 cut(s) 129, 188, 444
MboI GATC 3 cut(s) 127, 186, 442
MboII GAAGA 3 cut(s) 78, 431, 486
MflI RGATCY 2 cut(s) 127, 186
MluCI AATT 3 cut(s) 243, 268, 367
MlyI GAGTC 1 cut(s) 425
MnlI CCTC 7 cut(s) 121, 190, 224, 286, 369, 446, 449
MseI TTAA 2 cut(s) 242, 557
MslI CAYNNNNRTG 1 cut(s) 202
MspI CCGG 2 cut(s) 19, 318
MwoI GCNNNNNNNGC 1 cut(s) 15
NdeII GATC 3 cut(s) 127, 186, 442
NlaIII CATG 5 cut(s) 164, 195, 314, 487, 536
NlaIV GGNNCC 2 cut(s) 177, 178
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 91
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 542
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
Ppu21I YACGTR 2 cut(s) 103, 202
PpuMI RGGWCCY 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 176
PspN4I GGNNCC 2 cut(s) 177, 178
PspPI GGNCC 1 cut(s) 176
PspPPI RGGWCCY 1 cut(s) 176
PsuI RGATCY 2 cut(s) 127, 186
RsaI GTAC 2 cut(s) 470, 531
RsaNI GTAC 2 cut(s) 469, 530
RseI CAYNNNNRTG 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 91
SaqAI TTAA 2 cut(s) 242, 557
SatI GCNGC 1 cut(s) 541
Sau3AI GATC 3 cut(s) 127, 186, 442
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 425
SfaNI GCATC 1 cut(s) 270
SfcI CTRYAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 202
SmlI CTYRAG 2 cut(s) 131, 446
SmoI CTYRAG 2 cut(s) 131, 446
SnaBI TACGTA 1 cut(s) 103
Sse9I AATT 3 cut(s) 243, 268, 367
SsiI CCGC 1 cut(s) 326
SspMI CTAG 2 cut(s) 77, 348
TaaI ACNGT 3 cut(s) 121, 287, 529
TaiI ACGT 2 cut(s) 105, 204
TasI AATT 3 cut(s) 243, 268, 367
TatI WGTACW 2 cut(s) 468, 529
Tru1I TTAA 2 cut(s) 242, 557
Tru9I TTAA 2 cut(s) 242, 557
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 1 cut(s) 195
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 3 cut(s) 293, 414, 516
TspGWI ACGGA 2 cut(s) 55, 269
TspRI CASTG 1 cut(s) 532
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 176
XapI RAATTY 1 cut(s) 367
XspI CTAG 2 cut(s) 77, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.