FvH4_2g28951

ATP synthase B/B' CF(0)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009777
Physical Location & Seq
Reverse (-)
22572256 .. 22575060
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28951.t1

Sequence Viewer

Length: 1233 bp
ATGCGAAAGATTGATTATGATATTGAGGTTCCCAAGACAGCCACTGTTTTAGAACTGAAGCAGGCGGTGGAGGCGGTTTTCAGTCACATGCCACAGAAGGGACCTGGAAAGATATCATGGCCGCCTCCTGCAGGCATAATGCATGTCTATAGTTTAGAGCTTGATTTTGTTCGGCATGTTTCAATCAGCTACATAAATACAAAGAAGCAATCAAAAAGGTTCACTGGCTCAAAACAACACAGGAGGTCATTTAGTTTTGACGAGGAAGAACACAGCGAGCAAGAGGAAGAACAGAAGAACAAGGGCATAGAACAGGACGACAACATATACTACAATTGTGACGACATTGAGAATCAAAGGGTCCCGGAGTATGACAACGAACTTGCTGTTCAACAACACAGCGAGACCGGATTCCCATTCATACTGGGAGAGTGGTTTCCATACTCAAGACTATCACTGTCACCCGTAGGAGGAGGAGGAGGAAAATCTAGAAGAAGCTATGCTTGTTCCCCAGGCATAGCCACTGGGTTGTTGCTTGGCTTTAGGAAGATTTTCGGGCTTTGCTTCGAGAAACAAGCAGCTCAAGCTTTTGAAATCGCCATGGCCAACATGATCATGGCCTCCTCCAAGGTCCCAATCTCGGCCCCTGCTTCCGCCGTAGCCAAGCCCAAGCTCCTCCCTCTCCAAATCTCCATCCCCAAAACCCTAACCAAAACCCACCTCCTCCAGTCCCTCAAGTCCCTCTCCGTCATCGCCGCCACCTCCCTCGCCGTGGCGCCGCCGTCGCTCGCGGCGGAGATCGAGAAGGCGGCGCTGTTCGACTTTGACCTGACCCTACCGATCCAGGCCGTACAGTTCCTGCTGCTGATGGTGGCGCTGGACAAGGTGTACTACTCGCCGCTGGGGAAGTTCATGGACGAGCGCGACGCGGCGATCAGGGAGAAGCTGGGGAGCGTGAAGGACACGTCGGCGGAGGTGAAGCAGCTGGAGGAGCAAGGGGTGGCGATAATGAGGGCGGCGAGGGCGGAGATAGCGGCGGCGCTGTCGAAGATGAAGAAGGAGACGCAGGCGGAGGTGGAGGTGAAGCTGGCGGAGGGGAGGAAGAAGGTGGAGGGGGAGCTGCAGGAGGCGTTGGCGAAATTGGAGGAGCAGAAGGAGGAGACGTTGAAGGCCTTGGATGCCCAGATTGCTCAACTCAGTGACCAAATTGTGAAGAAGGTTCTTCCTCTGTGA

Protein Analysis

411

Amino Acids

45.41

Weight (kDa)

6.25

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_B PF00430 278 - 408 2.4e-12 ATP synthase B/B' CF(0)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 775
AccII CGCG 3 cut(s) 791, 924, 929
AclWI GGATC 1 cut(s) 835
AcoI YGGCCR 2 cut(s) 119, 603
AcuI CTGAAG 1 cut(s) 77
AcyI GRCGYC 1 cut(s) 776
AfaI GTAC 2 cut(s) 852, 890
AfiI CCNNNNNNNGG 5 cut(s) 98, 131, 470, 640, 772
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 4 cut(s) 183, 392, 593, 1168
AjiI CACGTC 1 cut(s) 966
AjnI CCWGG 3 cut(s) 103, 511, 843
Alw26I GTCTC 3 cut(s) 398, 1055, 1154
AlwI GGATC 1 cut(s) 835
AlwNI CAGNNNCTG 2 cut(s) 44, 859
AoxI GGCC 6 cut(s) 119, 603, 618, 642, 846, 1170
ApeKI GCWGC 4 cut(s) 578, 862, 982, 1120
Asp700I GAANNNNTTC 1 cut(s) 551
AspLEI GCGC 5 cut(s) 778, 814, 877, 924, 1042
AspS9I GGNCC 4 cut(s) 101, 361, 631, 643
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 3 cut(s) 453, 988, 1093
AvaII GGWCC 3 cut(s) 101, 361, 631
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 775
BbvI GCAGC 4 cut(s) 590, 849, 994, 1107
BccI CCATC 2 cut(s) 701, 862
BceAI ACGGC 4 cut(s) 641, 755, 766, 833
BciT130I CCWGG 3 cut(s) 105, 513, 845
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 3 cut(s) 398, 1055, 1154
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 3 cut(s) 129, 148, 1121
BfoI RGCGCY 4 cut(s) 779, 815, 878, 1043
Bme1390I CCNGG 4 cut(s) 105, 365, 513, 845
Bme18I GGWCC 3 cut(s) 101, 361, 631
BmgBI CACGTC 1 cut(s) 966
BmgT120I GGNCC 4 cut(s) 101, 361, 631, 643
BmiI GGNNCC 7 cut(s) 30, 102, 362, 363, 633, 645, 777
BmrFI CCNGG 4 cut(s) 105, 365, 513, 845
BmrI ACTGGG 2 cut(s) 434, 534
BmsI GCATC 1 cut(s) 1168
BmuI ACTGGG 2 cut(s) 434, 534
BpmI CTGGAG 2 cut(s) 710, 1007
BpuEI CTTGAG 3 cut(s) 430, 567, 719
BpuMI CCSGG 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 776
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 5 cut(s) 511, 600, 627, 771, 1173
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 2 cut(s) 1148, 1178
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 131, 470, 640, 772
Bse1I ACTGG 4 cut(s) 229, 429, 529, 727
BseBI CCWGG 3 cut(s) 105, 513, 845
BseDI CCNNGG 5 cut(s) 511, 600, 627, 771, 1173
BseGI GGATG 2 cut(s) 693, 1183
BseLI CCNNNNNNNGG 5 cut(s) 98, 131, 470, 640, 772
BseMII CTCAG 1 cut(s) 1210
BseNI ACTGG 4 cut(s) 229, 429, 529, 727
BseRI GAGGAG 9 cut(s) 486, 489, 492, 613, 665, 713, 1004, 1160, 1172
BseXI GCAGC 4 cut(s) 590, 849, 994, 1107
BseYI CCCAGC 2 cut(s) 901, 946
Bsh1236I CGCG 3 cut(s) 791, 924, 929
BshFI GGCC 6 cut(s) 121, 605, 620, 644, 848, 1172
BshNI GGYRCC 1 cut(s) 775
BsiSI CCGG 2 cut(s) 365, 408
BslFI GGGAC 5 cut(s) 114, 347, 617, 715, 724
BslI CCNNNNNNNGG 5 cut(s) 98, 131, 470, 640, 772
BsmAI GTCTC 3 cut(s) 398, 1055, 1154
BsmBI CGTCTC 2 cut(s) 1055, 1154
BsmFI GGGAC 5 cut(s) 114, 347, 617, 715, 724
BsnI GGCC 6 cut(s) 121, 605, 620, 644, 848, 1172
Bso31I GGTCTC 1 cut(s) 398
Bsp143I GATC 4 cut(s) 612, 798, 840, 933
Bsp19I CCATGG 1 cut(s) 600
BspANI GGCC 6 cut(s) 121, 605, 620, 644, 848, 1172
BspCNI CTCAG 1 cut(s) 1209
BspFNI CGCG 3 cut(s) 791, 924, 929
BspLI GGNNCC 7 cut(s) 30, 102, 362, 363, 633, 645, 777
BspMAI CTGCAG 2 cut(s) 133, 1125
BspPI GGATC 1 cut(s) 835
BspT107I GGYRCC 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 398
BsrI ACTGG 4 cut(s) 229, 429, 529, 727
BssECI CCNNGG 5 cut(s) 511, 600, 627, 771, 1173
BssMI GATC 4 cut(s) 612, 798, 840, 933
BssNI GRCGYC 1 cut(s) 776
BssT1I CCWWGG 3 cut(s) 600, 627, 1173
Bst2UI CCWGG 3 cut(s) 105, 513, 845
Bst4CI ACNGT 3 cut(s) 46, 459, 855
BstACI GRCGYC 1 cut(s) 776
BstC8I GCNNGC 6 cut(s) 63, 133, 278, 789, 1068, 1089
BstDEI CTNAG 1 cut(s) 1196
BstDSI CCRYGG 2 cut(s) 600, 771
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 693, 1183
BstFNI CGCG 3 cut(s) 791, 924, 929
BstH2I RGCGCY 4 cut(s) 779, 815, 878, 1043
BstHHI GCGC 5 cut(s) 778, 814, 877, 924, 1042
BstKTI GATC 4 cut(s) 615, 801, 843, 936
BstMAI GTCTC 3 cut(s) 398, 1055, 1154
BstMBI GATC 4 cut(s) 612, 798, 840, 933
BstMWI GCNNNNNNNGC 8 cut(s) 71, 584, 784, 991, 1022, 1031, 1178, 1187
BstNI CCWGG 3 cut(s) 105, 513, 845
BstNSI RCATGY 3 cut(s) 91, 146, 179
BstSCI CCNGG 4 cut(s) 103, 363, 511, 843
BstSFI CTRYAG 3 cut(s) 129, 148, 1121
BstUI CGCG 3 cut(s) 791, 924, 929
BstV1I GCAGC 4 cut(s) 590, 849, 994, 1107
BsuRI GGCC 6 cut(s) 121, 605, 620, 644, 848, 1172
BtgI CCRYGG 2 cut(s) 600, 771
BtgZI GCGATG 1 cut(s) 736
BtrI CACGTC 1 cut(s) 966
BtsCI GGATG 2 cut(s) 693, 1183
BtsIMutI CAGTG 5 cut(s) 42, 222, 455, 522, 1204
Cac8I GCNNGC 6 cut(s) 63, 133, 278, 789, 1068, 1089
CaiI CAGNNNCTG 2 cut(s) 44, 859
CfoI GCGC 5 cut(s) 778, 814, 877, 924, 1042
Cfr13I GGNCC 4 cut(s) 101, 361, 631, 643
CseI GACGC 2 cut(s) 935, 1072
Csp6I GTAC 2 cut(s) 851, 889
CviAII CATG 8 cut(s) 88, 117, 143, 176, 601, 610, 616, 913
CviQI GTAC 2 cut(s) 851, 889
DdeI CTNAG 1 cut(s) 1196
DinI GGCGCC 1 cut(s) 777
DpnI GATC 4 cut(s) 614, 800, 842, 935
DpnII GATC 4 cut(s) 612, 798, 840, 933
EaeI YGGCCR 2 cut(s) 119, 603
EciI GGCGGA 6 cut(s) 643, 809, 986, 1040, 1085, 1106
Eco130I CCWWGG 3 cut(s) 600, 627, 1173
Eco147I AGGCCT 1 cut(s) 1172
Eco31I GGTCTC 1 cut(s) 398
Eco32I GATATC 1 cut(s) 114
Eco47I GGWCC 3 cut(s) 101, 361, 631
Eco57I CTGAAG 1 cut(s) 77
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 3 cut(s) 101, 361, 631
EcoRII CCWGG 3 cut(s) 103, 511, 843
EcoRV GATATC 1 cut(s) 114
EcoT14I CCWWGG 3 cut(s) 600, 627, 1173
EcoT22I ATGCAT 1 cut(s) 144
EgeI GGCGCC 1 cut(s) 777
EheI GGCGCC 1 cut(s) 777
ErhI CCWWGG 3 cut(s) 600, 627, 1173
Esp3I CGTCTC 2 cut(s) 1055, 1154
FaeI CATG 8 cut(s) 91, 120, 146, 179, 604, 613, 619, 916
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FaqI GGGAC 5 cut(s) 114, 347, 617, 715, 724
FatI CATG 8 cut(s) 87, 116, 142, 175, 600, 609, 615, 912
FbaI TGATCA 1 cut(s) 612
FokI GGATG 2 cut(s) 680, 1190
FspBI CTAG 1 cut(s) 489
GlaI GCGC 5 cut(s) 777, 813, 876, 923, 1041
GsaI CCCAGC 2 cut(s) 905, 950
GsuI CTGGAG 2 cut(s) 710, 1007
HaeII RGCGCY 4 cut(s) 779, 815, 878, 1043
HaeIII GGCC 6 cut(s) 121, 605, 620, 644, 848, 1172
HapII CCGG 2 cut(s) 365, 408
HgaI GACGC 2 cut(s) 935, 1072
HhaI GCGC 5 cut(s) 778, 814, 877, 924, 1042
Hin1I GRCGYC 1 cut(s) 776
Hin1II CATG 8 cut(s) 91, 120, 146, 179, 604, 613, 619, 916
Hin6I GCGC 5 cut(s) 776, 812, 875, 922, 1040
HinP1I GCGC 5 cut(s) 776, 812, 875, 922, 1040
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 2 cut(s) 352, 411
HpaII CCGG 2 cut(s) 365, 408
HphI GGTGA 3 cut(s) 453, 988, 1093
Hpy166II GTNNAC 2 cut(s) 222, 889
Hpy188III TCNNGA 4 cut(s) 447, 489, 568, 802
Hpy8I GTNNAC 2 cut(s) 222, 889
Hpy99I CGWCG 3 cut(s) 787, 929, 970
HpyAV CCTTC 8 cut(s) 91, 799, 952, 1051, 1099, 1147, 1162, 1210
HpyCH4III ACNGT 3 cut(s) 46, 459, 855
HpyCH4IV ACGT 2 cut(s) 965, 1163
HpyCH4V TGCA 3 cut(s) 131, 142, 1123
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 584, 784, 991, 1022, 1031, 1178, 1187
HpyF3I CTNAG 1 cut(s) 1196
HpySE526I ACGT 2 cut(s) 965, 1163
Hsp92I GRCGYC 1 cut(s) 776
Hsp92II CATG 8 cut(s) 91, 120, 146, 179, 604, 613, 619, 916
HspAI GCGC 5 cut(s) 776, 812, 875, 922, 1040
KasI GGCGCC 1 cut(s) 775
KflI GGGWCCC 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 4 cut(s) 612, 798, 840, 933
LmnI GCTCC 5 cut(s) 678, 951, 991, 1117, 1147
Lsp1109I GCAGC 4 cut(s) 590, 849, 994, 1107
LweI GCATC 1 cut(s) 1168
MaeI CTAG 1 cut(s) 489
MaeII ACGT 2 cut(s) 965, 1163
MaeIII GTNAC 4 cut(s) 83, 338, 459, 1199
MalI GATC 4 cut(s) 614, 800, 842, 935
MboI GATC 4 cut(s) 612, 798, 840, 933
MfeI CAATTG 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 3 cut(s) 334, 1139, 1206
MluNI TGGCCA 1 cut(s) 605
Mly113I GGCGCC 1 cut(s) 776
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 551
MscI TGGCCA 1 cut(s) 605
MslI CAYNNNNRTG 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 2 cut(s) 901, 985
MspI CCGG 2 cut(s) 365, 408
MspR9I CCNGG 4 cut(s) 105, 365, 513, 845
MunI CAATTG 1 cut(s) 334
MvaI CCWGG 3 cut(s) 105, 513, 845
MvnI CGCG 3 cut(s) 791, 924, 929
MwoI GCNNNNNNNGC 8 cut(s) 71, 584, 784, 991, 1022, 1031, 1178, 1187
NarI GGCGCC 1 cut(s) 776
NciI CCSGG 1 cut(s) 365
NcoI CCATGG 1 cut(s) 600
NdeII GATC 4 cut(s) 612, 798, 840, 933
NlaIII CATG 8 cut(s) 91, 120, 146, 179, 604, 613, 619, 916
NlaIV GGNNCC 7 cut(s) 30, 102, 362, 363, 633, 645, 777
NmeAIII GCCGAG 1 cut(s) 620
NmuCI GTSAC 4 cut(s) 83, 338, 459, 1199
NsiI ATGCAT 1 cut(s) 144
NspI RCATGY 3 cut(s) 91, 146, 179
PceI AGGCCT 1 cut(s) 1172
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 2 cut(s) 352, 411
PfoI TCCNGGA 1 cut(s) 363
PluTI GGCGCC 1 cut(s) 779
PpuMI RGGWCCY 3 cut(s) 101, 361, 631
Psp5II RGGWCCY 3 cut(s) 101, 361, 631
Psp6I CCWGG 3 cut(s) 103, 511, 843
PspFI CCCAGC 2 cut(s) 901, 946
PspGI CCWGG 3 cut(s) 103, 511, 843
PspN4I GGNNCC 7 cut(s) 30, 102, 362, 363, 633, 645, 777
PspPI GGNCC 4 cut(s) 101, 361, 631, 643
PspPPI RGGWCCY 3 cut(s) 101, 361, 631
PstI CTGCAG 2 cut(s) 133, 1125
PstNI CAGNNNCTG 2 cut(s) 44, 859
PvuII CAGCTG 1 cut(s) 985
RsaI GTAC 2 cut(s) 852, 890
RsaNI GTAC 2 cut(s) 851, 889
RseI CAYNNNNRTG 1 cut(s) 614
Sau3AI GATC 4 cut(s) 612, 798, 840, 933
Sau96I GGNCC 4 cut(s) 101, 361, 631, 643
SbfI CCTGCAGG 1 cut(s) 133
ScrFI CCNGG 4 cut(s) 105, 365, 513, 845
SdaI CCTGCAGG 1 cut(s) 133
SfaNI GCATC 1 cut(s) 1168
SfcI CTRYAG 3 cut(s) 129, 148, 1121
SfoI GGCGCC 1 cut(s) 777
SinI GGWCC 3 cut(s) 101, 361, 631
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 3 cut(s) 445, 582, 734
SmoI CTYRAG 3 cut(s) 445, 582, 734
Sse8387I CCTGCAGG 1 cut(s) 133
Sse9I AATT 3 cut(s) 334, 1139, 1206
SseBI AGGCCT 1 cut(s) 1172
SspDI GGCGCC 1 cut(s) 775
SspMI CTAG 1 cut(s) 489
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 4 cut(s) 103, 363, 511, 843
StyI CCWWGG 3 cut(s) 600, 627, 1173
TaaI ACNGT 3 cut(s) 46, 459, 855
TaiI ACGT 2 cut(s) 968, 1166
TaqI TCGA 4 cut(s) 567, 801, 819, 1046
TasI AATT 3 cut(s) 334, 1139, 1206
TatI WGTACW 1 cut(s) 888
TfiI GAWTC 2 cut(s) 352, 411
TscAI CASTG 5 cut(s) 49, 229, 462, 529, 1204
TseFI GTSAC 4 cut(s) 83, 338, 459, 1199
TseI GCWGC 4 cut(s) 578, 862, 982, 1120
Tsp45I GTSAC 4 cut(s) 83, 338, 459, 1199
TspDTI ATGAA 3 cut(s) 409, 901, 1067
TspGWI ACGGA 1 cut(s) 736
TspRI CASTG 5 cut(s) 49, 229, 462, 529, 1204
VpaK11BI GGWCC 3 cut(s) 101, 361, 631
XagI CCTNNNNNAGG 1 cut(s) 129
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 3 cut(s) 91, 146, 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 1 cut(s) 489
Zsp2I ATGCAT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.