MD08G1155200.v1.1

ATP synthesis coupled proton transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009777
Physical Location & Seq
Forward (+)
17261139 .. 17263875
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1155200.v1.1.491

Sequence Viewer

Length: 861 bp
ATGTCGTATAGCACGCTGAGGATTGACGATGATGATTTTGTCCCTGTCCGGCAGCCGTCGATCGAACGGCATCGCCGCTCCAATTCTCTGCCGTTTGTGCCGTTGCTGCTCGTCGATGGCTTTTCCCGCAAGAGCTTCTCCTACTCCGCGCTTCCTCAGGAGCCACTCCGGCTCTCCGTTATGAAATTGGACGGCTCTTGCTTCGGTTATATTCAAGTTGCTATGACGGCCACGGTTTTAGAATTGAAGCAGGCAGTTGAGGCCGTTTTCAGTCACATGCCACAGAAGGGACCGGGAAAAATTTCATGGCCACACGTGTGGGGTCATTTCTGCTTGTGCTACGGTAATCAGAAGCTACTTGTTGAAACTGATCATATCAACCATTATGGGATCAAAGATGGTGATCAGCTGCATTTTGTCCGGCATGTTTCAATCAGCTACAGCATGACAAAGAGGCAATCGTCAAAGAAAGGGTTCCTCGCTTTAAAACAAAACACAATGTCAGTGTCGCGATCAATGAGCTTTCAAGAGGAAGGGCAGACTGACATACAGGAAGAAGGGAATGGCAAAGAGGTAGAACAAAACGACGAGGTATATTATGATTCTGATGACATCGAGAATCCAAAGTCCCCACTCTGTGATGACGAAGTTTCTATTGACCACAATGGGAGCAGGCTGCCTTTCTTATTAGGAGAATGGTTTCCATACTCCAAACTGTCAGCTGTAGGAACATCGTCTCCAAGAACTTGCGCGCCAAGCATAGCTAGTGGCTTACTGGTTGGGTTTAGGAAGATATTCGGGCTTTGTTTCGAAAAACGTGATAAACGGCACAGTCGAAGGGATACTTGGAGAATAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

287

Amino Acids

32.53

Weight (kDa)

6.8

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 56 - 143 3.6e-24 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 78
AccII CGCG 3 cut(s) 149, 511, 752
AciI CCGC 3 cut(s) 76, 127, 147
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 2 cut(s) 228, 308
AcsI RAATTY 1 cut(s) 300
AcvI CACGTG 1 cut(s) 316
AdeI CACNNNGTG 1 cut(s) 638
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 2 cut(s) 313, 315
AgsI TTSAA 5 cut(s) 215, 247, 365, 432, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 829, 861
AleI CACNNNNGTG 1 cut(s) 316
AloI GAACNNNNNNTCC 2 cut(s) 721, 753
AluBI AGCT 7 cut(s) 135, 355, 409, 438, 522, 722, 764
AluI AGCT 7 cut(s) 135, 355, 409, 438, 522, 722, 764
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 1 cut(s) 398
AoxI GGCC 3 cut(s) 228, 261, 308
ApeKI GCWGC 4 cut(s) 52, 106, 409, 676
ApoI RAATTY 1 cut(s) 300
Asp700I GAANNNNTTC 4 cut(s) 301, 473, 699, 794
AspLEI GCGC 3 cut(s) 151, 752, 754
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 413
AsuII TTCGAA 1 cut(s) 810
AvaII GGWCC 1 cut(s) 290
AxyI CCTNAGG 1 cut(s) 156
BalI TGGCCA 1 cut(s) 310
BbrPI CACGTG 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 17
BbvI GCAGC 4 cut(s) 64, 93, 396, 663
BccI CCATC 2 cut(s) 110, 392
BceAI ACGGC 8 cut(s) 40, 76, 83, 85, 208, 243, 248, 842
BciVI GTATCC 1 cut(s) 835
BclI TGATCA 2 cut(s) 370, 403
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 1 cut(s) 765
BfmI CTRYAG 2 cut(s) 439, 723
BfuI GTATCC 1 cut(s) 835
BglI GCCNNNNNGGC 1 cut(s) 169
BisI GCNGC 5 cut(s) 53, 76, 107, 410, 677
BlsI GCNGC 5 cut(s) 54, 77, 108, 411, 678
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 3 cut(s) 162, 291, 476
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 79
Bpu10I CCTNAGC 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 810
BpuMI CCSGG 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 316
BsaBI GATNNNNATC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 721, 751
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 780
Bse21I CCTNAGG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 231
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMII CTCAG 2 cut(s) 8, 170
BseNI ACTGG 1 cut(s) 780
BsePI GCGCGC 1 cut(s) 750
BseXI GCAGC 4 cut(s) 64, 93, 396, 663
Bsh1236I CGCG 3 cut(s) 149, 511, 752
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 3 cut(s) 230, 263, 310
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 4 cut(s) 49, 169, 293, 421
BslFI GGGAC 3 cut(s) 26, 303, 613
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 741
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 3 cut(s) 26, 303, 613
BsnI GGCC 3 cut(s) 230, 263, 310
Bsp119I TTCGAA 1 cut(s) 810
Bsp143I GATC 5 cut(s) 60, 370, 390, 403, 512
Bsp68I TCGCGA 1 cut(s) 511
BspACI CCGC 3 cut(s) 76, 127, 147
BspANI GGCC 3 cut(s) 230, 263, 310
BspCNI CTCAG 2 cut(s) 9, 169
BspFNI CGCG 3 cut(s) 149, 511, 752
BspLI GGNNCC 3 cut(s) 162, 291, 476
BspPI GGATC 1 cut(s) 398
BspT104I TTCGAA 1 cut(s) 810
BsrBI CCGCTC 1 cut(s) 78
BsrI ACTGG 1 cut(s) 780
BssECI CCNNGG 1 cut(s) 231
BssHII GCGCGC 1 cut(s) 750
BssMI GATC 5 cut(s) 60, 370, 390, 403, 512
Bst4CI ACNGT 4 cut(s) 235, 344, 717, 833
BstBAI YACGTR 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 810
BstC8I GCNNGC 4 cut(s) 14, 252, 674, 752
BstDEI CTNAG 2 cut(s) 17, 156
BstDSI CCRYGG 1 cut(s) 231
BstFNI CGCG 3 cut(s) 149, 511, 752
BstHHI GCGC 3 cut(s) 151, 752, 754
BstKTI GATC 5 cut(s) 63, 373, 393, 406, 515
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 5 cut(s) 60, 370, 390, 403, 512
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 7 cut(s) 97, 106, 126, 169, 227, 260, 756
BstNSI RCATGY 2 cut(s) 280, 428
BstSCI CCNGG 1 cut(s) 292
BstSFI CTRYAG 2 cut(s) 439, 723
BstUI CGCG 3 cut(s) 149, 511, 752
BstV1I GCAGC 4 cut(s) 64, 93, 396, 663
BstXI CCANNNNNNTGG 1 cut(s) 318
Bsu36I CCTNAGG 1 cut(s) 156
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 3 cut(s) 230, 263, 310
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 56
BtsIMutI CAGTG 1 cut(s) 510
BtuMI TCGCGA 1 cut(s) 511
Cac8I GCNNGC 4 cut(s) 14, 252, 674, 752
CfoI GCGC 3 cut(s) 151, 752, 754
Cfr13I GGNCC 1 cut(s) 290
CviAII CATG 4 cut(s) 277, 306, 425, 445
DdeI CTNAG 2 cut(s) 17, 156
DpnI GATC 5 cut(s) 62, 372, 392, 405, 514
DpnII GATC 5 cut(s) 60, 370, 390, 403, 512
DraI TTTAAA 1 cut(s) 486
DraIII CACNNNGTG 1 cut(s) 638
EaeI YGGCCR 2 cut(s) 228, 308
Eco47I GGWCC 1 cut(s) 290
Eco72I CACGTG 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 4 cut(s) 280, 309, 428, 448
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FaqI GGGAC 3 cut(s) 26, 303, 613
FatI CATG 4 cut(s) 276, 305, 424, 444
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 2 cut(s) 370, 403
Fnu4HI GCNGC 5 cut(s) 53, 76, 107, 410, 677
Fsp4HI GCNGC 5 cut(s) 53, 76, 107, 410, 677
FspBI CTAG 1 cut(s) 765
GlaI GCGC 3 cut(s) 150, 751, 753
GluI GCNGC 5 cut(s) 53, 76, 107, 410, 677
HaeIII GGCC 3 cut(s) 230, 263, 310
HapII CCGG 4 cut(s) 49, 169, 293, 421
HhaI GCGC 3 cut(s) 151, 752, 754
Hin1II CATG 4 cut(s) 280, 309, 428, 448
Hin6I GCGC 3 cut(s) 149, 750, 752
HinP1I GCGC 3 cut(s) 149, 750, 752
HinfI GANTC 2 cut(s) 602, 619
HpaII CCGG 4 cut(s) 49, 169, 293, 421
HphI GGTGA 1 cut(s) 413
Hpy188I TCNGA 2 cut(s) 351, 607
Hpy188III TCNNGA 4 cut(s) 158, 510, 527, 616
Hpy99I CGWCG 3 cut(s) 61, 116, 590
HpyAV CCTTC 4 cut(s) 280, 527, 551, 831
HpyCH4III ACNGT 4 cut(s) 235, 344, 717, 833
HpyCH4IV ACGT 2 cut(s) 315, 817
HpyCH4V TGCA 1 cut(s) 412
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 106, 126, 169, 227, 260, 756
HpyF3I CTNAG 2 cut(s) 17, 156
HpySE526I ACGT 2 cut(s) 315, 817
Hsp92II CATG 4 cut(s) 280, 309, 428, 448
HspAI GCGC 3 cut(s) 149, 750, 752
Ksp22I TGATCA 2 cut(s) 370, 403
Kzo9I GATC 5 cut(s) 60, 370, 390, 403, 512
LmnI GCTCC 3 cut(s) 83, 160, 669
Lsp1109I GCAGC 4 cut(s) 64, 93, 396, 663
LweI GCATC 1 cut(s) 79
MaeI CTAG 1 cut(s) 765
MaeII ACGT 2 cut(s) 315, 817
MaeIII GTNAC 1 cut(s) 272
MalI GATC 5 cut(s) 62, 372, 392, 405, 514
MbiI CCGCTC 1 cut(s) 78
MboI GATC 5 cut(s) 60, 370, 390, 403, 512
MboII GAAGA 2 cut(s) 566, 802
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 4 cut(s) 82, 185, 242, 300
MluNI TGGCCA 1 cut(s) 310
MnlI CCTC 8 cut(s) 12, 165, 253, 447, 488, 523, 565, 583
Mox20I TGGCCA 1 cut(s) 310
MroXI GAANNNNTTC 4 cut(s) 301, 473, 699, 794
MscI TGGCCA 1 cut(s) 310
MseI TTAA 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 316
Msp20I TGGCCA 1 cut(s) 310
MspA1I CMGCKG 2 cut(s) 409, 722
MspI CCGG 4 cut(s) 49, 169, 293, 421
MspR9I CCNGG 1 cut(s) 294
MvnI CGCG 3 cut(s) 149, 511, 752
MwoI GCNNNNNNNGC 7 cut(s) 97, 106, 126, 169, 227, 260, 756
NciI CCSGG 1 cut(s) 294
NdeII GATC 5 cut(s) 60, 370, 390, 403, 512
NlaIII CATG 4 cut(s) 280, 309, 428, 448
NlaIV GGNNCC 3 cut(s) 162, 291, 476
NmuCI GTSAC 1 cut(s) 272
NruI TCGCGA 1 cut(s) 511
NspI RCATGY 2 cut(s) 280, 428
NspV TTCGAA 1 cut(s) 810
OliI CACNNNNGTG 1 cut(s) 316
PauI GCGCGC 1 cut(s) 750
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PdmI GAANNNNTTC 4 cut(s) 301, 473, 699, 794
PfeI GAWTC 2 cut(s) 602, 619
PkrI GCNGC 5 cut(s) 54, 77, 108, 411, 678
Ple19I CGATCG 1 cut(s) 63
PmaCI CACGTG 1 cut(s) 316
PmlI CACGTG 1 cut(s) 316
Ppu21I YACGTR 1 cut(s) 316
PspCI CACGTG 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 162, 291, 476
PspPI GGNCC 1 cut(s) 290
PteI GCGCGC 1 cut(s) 750
PvuI CGATCG 1 cut(s) 63
PvuII CAGCTG 2 cut(s) 409, 722
RruI TCGCGA 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 316
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 5 cut(s) 53, 76, 107, 410, 677
Sau3AI GATC 5 cut(s) 60, 370, 390, 403, 512
Sau96I GGNCC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 294
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 2 cut(s) 439, 723
SfuI TTCGAA 1 cut(s) 810
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 316
Sse9I AATT 4 cut(s) 82, 185, 242, 300
SsiI CCGC 3 cut(s) 76, 127, 147
SspMI CTAG 1 cut(s) 765
StyD4I CCNGG 1 cut(s) 292
TaaI ACNGT 4 cut(s) 235, 344, 717, 833
TaiI ACGT 2 cut(s) 318, 820
TaqI TCGA 6 cut(s) 59, 63, 114, 615, 810, 835
TasI AATT 4 cut(s) 82, 185, 242, 300
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 2 cut(s) 602, 619
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 4 cut(s) 52, 106, 409, 676
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 197, 294
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 1 cut(s) 300
XceI RCATGY 2 cut(s) 280, 428
XmnI GAANNNNTTC 4 cut(s) 301, 473, 699, 794
XspI CTAG 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.