AT4G32780

Auxin canalisation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007321
Physical Location & Seq
Forward (+)
15808852 .. 15811926
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32780.1

Sequence Viewer

Length: 1224 bp
ATGATGTGCCACACGGGAAGAGACAATTCATGGAAAAAACTAGAAAACATAGACGAAGAAGGTCCATCGACGGTGGCACGTGAAGTGAGGACGGCGTTACCACCGGAGACGCCAACGGAGATGATGGAGTTCTTGGGGAGATCATGGAGCATTTCCGCCGTCGAACTCACGAGAGCTTTTTTCAACAATTCCACTGCCGACACTAACTCTTTTCTTCTATCTACTATCGTTAACACCAACAAAGAAGACAGAGAAGATGAAGAAGATTCTACTTCCATGGCTTCTTCTCGTGACCTTCTGCTGCCACATGTGGGAAATAAGACCAGCCCTCCAATAACTCCAAGAACCGGTAGAGAAATGAAGCATTTGTACAAGAGCATGATAAGAGGAAGAACAATGGGAAGAAGATTAAAGGATCAGAAGGAGAAGAAGAAACAAGAGACCAGAACTCGCAACGCTGAGATTCACGCCGCGGTCTCTGTCGCAGGAGTAGCTGCAGTTGTTGCCGCAACCGCAGCATCTAACGCTATTGCGGCCGCAGAAGATGCGGTTGAATCAACAACCGTTGCAGCTGCAATGGCATCTGCAGCTGCTCTCATCGCTTCACATTGCATCGAGATAGCGGGAGAGATTGGGGCGGGTTATAACCAGATTGCCACGGCTGTTAGCTCCGCGACCAATGCTAGGACTAACGGAGACATTATGGCTTTAACGGCAAGTGCTGCAACAGCGTTGCGAGGAGCTGCGATTCTAAGGTCACGAATGGAAAGGAACAACGAGAACAAAGCAATGCTATATGCAACAAAGGAGCACGTCGTAGAAATGGAAGAGAGAAATGTATTTGGAGCGATGACTTTCGTCTCTAGAGGAGAAGAGCTTCTCAAACGCACCCGCAAAGGGGATCTTCATTGGAAGCAAGTATCTTTTAACATCAATTCCAATTGGCAAGTGGTTCTTAAAATGAAGAGCAAACATGTGGGTGGAACTTTCACGAAGACGAAGAAATGCGTTGTCAATGGAGTGTGCCGAGATATACCCGAATGGGCACATAGAGGACGAGCAGATAAAATGGTAGAGCGAAGAGCCTACTTTGGGGTGAAAACAGTGGAAAGGGTGATTGAATTCGAATGTGGAAACAAAAGAGAGAAGCAAATGTGGATAGAGGGGATTCAACAGTTGCTTAACTCACTCGAAATCGGTTCTAGTGTTCAATGGAAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_canalis PH_2

Protein Analysis

407

Amino Acids

45.03

Weight (kDa)

9.26

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_canalis PF05703 35 - 265 1.1e-66 Auxin canalisation
PH_2 PF08458 292 - 396 1.3e-42 Plant pleckstrin homology-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AccII CGCG 2 cut(s) 473, 674
AclWI GGATC 2 cut(s) 423, 909
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 1 cut(s) 1121
AcvI CACGTG 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 110
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 6 cut(s) 311, 347, 684, 897, 898, 1092
AflIII ACRYGT 2 cut(s) 307, 973
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 5 cut(s) 184, 554, 1121, 1172, 1211
AjiI CACGTC 1 cut(s) 814
AluBI AGCT 7 cut(s) 176, 494, 572, 590, 669, 743, 877
AluI AGCT 7 cut(s) 176, 494, 572, 590, 669, 743, 877
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 6 cut(s) 15, 101, 434, 481, 690, 865
AlwI GGATC 2 cut(s) 423, 909
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 9 cut(s) 301, 494, 515, 569, 572, 587, 590, 722, 743
ApoI RAATTY 1 cut(s) 1121
AsiGI ACCGGT 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 876
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 1108, 1126
AsuII TTCGAA 1 cut(s) 1125
AvaII GGWCC 1 cut(s) 62
BaeGI GKGCMC 1 cut(s) 1048
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BauI CACGAG 2 cut(s) 169, 288
BbrPI CACGTG 1 cut(s) 80
BbsI GAAGAC 2 cut(s) 252, 1001
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 9 cut(s) 288, 481, 527, 559, 577, 581, 599, 709, 730
BccI CCATC 2 cut(s) 73, 118
BceAI ACGGC 4 cut(s) 108, 143, 675, 729
BcoDI GTCTC 6 cut(s) 15, 101, 434, 481, 690, 865
BfaI CTAG 4 cut(s) 41, 684, 864, 1203
BfmI CTRYAG 2 cut(s) 495, 585
Bme18I GGWCC 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 1 cut(s) 62
BmsI GCATC 4 cut(s) 527, 535, 590, 621
BoxI GACNNNNGTC 1 cut(s) 857
BpiI GAAGAC 2 cut(s) 252, 1001
Bpu14I TTCGAA 1 cut(s) 1125
BsaAI YACGTR 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 110
BsaI GGTCTC 2 cut(s) 434, 481
BsaJI CCNNGG 3 cut(s) 276, 471, 657
BsaWI WCCGGW 2 cut(s) 103, 347
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 6 cut(s) 311, 347, 684, 897, 898, 1092
Bse118I RCCGGY 1 cut(s) 347
Bse3DI GCAATG 3 cut(s) 582, 607, 795
BseDI CCNNGG 3 cut(s) 276, 471, 657
BseLI CCNNNNNNNGG 6 cut(s) 311, 347, 684, 897, 898, 1092
BseMI GCAATG 3 cut(s) 582, 607, 795
BseMII CTCAG 1 cut(s) 450
BseRI GAGGAG 2 cut(s) 753, 882
BseSI GKGCMC 1 cut(s) 1048
BseX3I CGGCCG 1 cut(s) 534
BseXI GCAGC 9 cut(s) 288, 481, 527, 559, 577, 581, 599, 709, 730
Bsh1236I CGCG 2 cut(s) 473, 674
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 1 cut(s) 536
BshTI ACCGGT 1 cut(s) 347
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 1 cut(s) 813
BsiSI CCGG 2 cut(s) 104, 348
BslI CCNNNNNNNGG 6 cut(s) 311, 347, 684, 897, 898, 1092
BsmAI GTCTC 6 cut(s) 15, 101, 434, 481, 690, 865
BsmBI CGTCTC 2 cut(s) 101, 865
BsnI GGCC 1 cut(s) 536
Bso31I GGTCTC 2 cut(s) 434, 481
Bsp119I TTCGAA 1 cut(s) 1125
Bsp1286I GDGCHC 2 cut(s) 813, 1048
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 3 cut(s) 140, 415, 901
Bsp19I CCATGG 1 cut(s) 276
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 1 cut(s) 451
BspFNI CGCG 2 cut(s) 473, 674
BspMAI CTGCAG 2 cut(s) 499, 589
BspPI GGATC 2 cut(s) 423, 909
BspQI GCTCTTC 3 cut(s) 867, 959, 1075
BspT104I TTCGAA 1 cut(s) 1125
BspTNI GGTCTC 2 cut(s) 434, 481
BsrDI GCAATG 3 cut(s) 582, 607, 795
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 3 cut(s) 276, 471, 657
BssMI GATC 3 cut(s) 140, 415, 901
BssNI GRCGYC 1 cut(s) 110
BssSI CACGAG 2 cut(s) 169, 288
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 2 cut(s) 169, 288
Bst4CI ACNGT 4 cut(s) 73, 565, 1105, 1176
Bst6I CTCTTC 5 cut(s) 13, 822, 867, 959, 1075
BstACI GRCGYC 1 cut(s) 110
BstAPI GCANNNNNTGC 3 cut(s) 503, 545, 722
BstAUI TGTACA 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 1125
BstDEI CTNAG 2 cut(s) 459, 752
BstDSI CCRYGG 3 cut(s) 276, 471, 657
BstFNI CGCG 2 cut(s) 473, 674
BstKTI GATC 3 cut(s) 143, 418, 904
BstMAI GTCTC 6 cut(s) 15, 101, 434, 481, 690, 865
BstMBI GATC 3 cut(s) 140, 415, 901
BstMCI CGRYCG 1 cut(s) 537
BstNSI RCATGY 2 cut(s) 311, 977
BstPAI GACNNNNGTC 1 cut(s) 857
BstSFI CTRYAG 2 cut(s) 495, 585
BstSLI GKGCMC 1 cut(s) 1048
BstUI CGCG 2 cut(s) 473, 674
BstV1I GCAGC 9 cut(s) 288, 481, 527, 559, 577, 581, 599, 709, 730
BstV2I GAAGAC 2 cut(s) 252, 1001
BstX2I RGATCY 1 cut(s) 901
BstYI RGATCY 1 cut(s) 901
BstZI CGGCCG 1 cut(s) 534
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 3 cut(s) 276, 471, 657
BtgZI GCGATG 2 cut(s) 583, 863
BtrI CACGTC 1 cut(s) 814
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 192, 1110
CciNI GCGGCCGC 1 cut(s) 534
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 62
Cfr42I CCGCGG 1 cut(s) 474
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 370
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 6 cut(s) 30, 144, 277, 308, 379, 974
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 2 cut(s) 459, 752
DpnI GATC 3 cut(s) 142, 417, 903
DpnII GATC 3 cut(s) 140, 415, 901
EaeI YGGCCR 1 cut(s) 534
EagI CGGCCG 1 cut(s) 534
Eam1104I CTCTTC 5 cut(s) 13, 822, 867, 959, 1075
EarI CTCTTC 5 cut(s) 13, 822, 867, 959, 1075
EciI GGCGGA 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 276
Eco31I GGTCTC 2 cut(s) 434, 481
Eco47I GGWCC 1 cut(s) 62
Eco52I CGGCCG 1 cut(s) 534
Eco72I CACGTG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 1121
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 2 cut(s) 101, 865
FaeI CATG 6 cut(s) 33, 147, 280, 311, 382, 977
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 939, 971
FatI CATG 6 cut(s) 29, 143, 276, 307, 378, 973
FauI CCCGC 3 cut(s) 616, 631, 899
FspBI CTAG 4 cut(s) 41, 684, 864, 1203
HaeIII GGCC 1 cut(s) 536
HapII CCGG 2 cut(s) 104, 348
HgaI GACGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 6 cut(s) 33, 147, 280, 311, 382, 977
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 5 cut(s) 266, 463, 554, 748, 1168
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 2 cut(s) 104, 348
HphI GGTGA 2 cut(s) 1108, 1126
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 6 cut(s) 169, 290, 616, 759, 864, 991
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 3 cut(s) 73, 164, 818
HpyAV CCTTC 3 cut(s) 53, 305, 415
HpyCH4III ACNGT 4 cut(s) 73, 565, 1105, 1176
HpyCH4IV ACGT 2 cut(s) 79, 813
HpyCH4V TGCA 7 cut(s) 497, 569, 575, 587, 612, 725, 800
HpyF3I CTNAG 2 cut(s) 459, 752
HpySE526I ACGT 2 cut(s) 79, 813
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 6 cut(s) 33, 147, 280, 311, 382, 977
KspAI GTTAAC 1 cut(s) 232
KspI CCGCGG 1 cut(s) 474
Kzo9I GATC 3 cut(s) 140, 415, 901
LguI GCTCTTC 3 cut(s) 867, 959, 1075
LmnI GCTCC 5 cut(s) 147, 674, 740, 808, 845
LpnPI CCDG 6 cut(s) 117, 337, 361, 457, 471, 662
Lsp1109I GCAGC 9 cut(s) 288, 481, 527, 559, 577, 581, 599, 709, 730
LweI GCATC 4 cut(s) 527, 535, 590, 621
MaeI CTAG 4 cut(s) 41, 684, 864, 1203
MaeII ACGT 2 cut(s) 79, 813
MaeIII GTNAC 3 cut(s) 96, 290, 756
MalI GATC 3 cut(s) 142, 417, 903
MboI GATC 3 cut(s) 140, 415, 901
MfeI CAATTG 1 cut(s) 940
MflI RGATCY 1 cut(s) 901
MhlI GDGCHC 2 cut(s) 813, 1048
MluCI AATT 5 cut(s) 25, 187, 934, 940, 1121
MnlI CCTC 7 cut(s) 81, 339, 380, 731, 860, 1046, 1156
MroXI GAANNNNTTC 1 cut(s) 876
MseI TTAA 6 cut(s) 231, 410, 710, 927, 957, 1182
MslI CAYNNNNRTG 1 cut(s) 978
MspA1I CMGCKG 3 cut(s) 473, 572, 590
MspI CCGG 2 cut(s) 104, 348
MunI CAATTG 1 cut(s) 940
MvnI CGCG 2 cut(s) 473, 674
NcoI CCATGG 1 cut(s) 276
NdeII GATC 3 cut(s) 140, 415, 901
NlaIII CATG 6 cut(s) 33, 147, 280, 311, 382, 977
NmeAIII GCCGAG 1 cut(s) 1052
NmuCI GTSAC 2 cut(s) 290, 756
NotI GCGGCCGC 1 cut(s) 534
NspI RCATGY 2 cut(s) 311, 977
NspV TTCGAA 1 cut(s) 1125
PciI ACATGT 2 cut(s) 307, 973
PciSI GCTCTTC 3 cut(s) 867, 959, 1075
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 5 cut(s) 266, 463, 554, 748, 1168
PinAI ACCGGT 1 cut(s) 347
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 80
PscI ACATGT 2 cut(s) 307, 973
PshAI GACNNNNGTC 1 cut(s) 857
PsiI TTATAA 1 cut(s) 645
PspCI CACGTG 1 cut(s) 80
PspPI GGNCC 1 cut(s) 62
PstI CTGCAG 2 cut(s) 499, 589
PsuI RGATCY 1 cut(s) 901
PvuII CAGCTG 2 cut(s) 572, 590
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 978
SacII CCGCGG 1 cut(s) 474
SapI GCTCTTC 3 cut(s) 867, 959, 1075
SaqAI TTAA 6 cut(s) 231, 410, 710, 927, 957, 1182
Sau3AI GATC 3 cut(s) 140, 415, 901
Sau96I GGNCC 1 cut(s) 62
SduI GDGCHC 2 cut(s) 813, 1048
SfaNI GCATC 4 cut(s) 527, 535, 590, 621
SfcI CTRYAG 2 cut(s) 495, 585
Sfr303I CCGCGG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 1125
SgrBI CCGCGG 1 cut(s) 474
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 978
Sse9I AATT 5 cut(s) 25, 187, 934, 940, 1121
SspMI CTAG 4 cut(s) 41, 684, 864, 1203
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 4 cut(s) 73, 565, 1105, 1176
TaiI ACGT 2 cut(s) 82, 816
TaqI TCGA 5 cut(s) 68, 162, 615, 1125, 1191
TasI AATT 5 cut(s) 25, 187, 934, 940, 1121
TatI WGTACW 1 cut(s) 369
TauI GCSGC 4 cut(s) 473, 509, 536, 539
TfiI GAWTC 5 cut(s) 266, 463, 554, 748, 1168
Tru1I TTAA 6 cut(s) 231, 410, 710, 927, 957, 1182
Tru9I TTAA 6 cut(s) 231, 410, 710, 927, 957, 1182
TscAI CASTG 2 cut(s) 199, 1110
TseFI GTSAC 2 cut(s) 290, 756
TseI GCWGC 9 cut(s) 301, 494, 515, 569, 572, 587, 590, 722, 743
Tsp45I GTSAC 2 cut(s) 290, 756
TspDTI ATGAA 5 cut(s) 18, 273, 374, 896, 977
TspGWI ACGGA 2 cut(s) 131, 708
TspRI CASTG 2 cut(s) 199, 1110
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 1 cut(s) 1121
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 2 cut(s) 311, 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 4 cut(s) 41, 684, 864, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.