RchiOBHm_Chr6g0306061

Plant pleckstrin homology-like region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007321
Physical Location & Seq
Forward (+)
64819245 .. 64822358
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27508

Sequence Viewer

Length: 1386 bp
ATGCACCCTACTTTTTCGTCCTTGTTAAATGCGTACTGTGCTGTTGTACCTCCTCTTCATCACCATCGTCTTATTCTAGCTTTCTTTGCTAAACTAGATTCTCCATTGGTAGCCGAGCTTGAAAACTCTTGTAGTTTGAAATGCTCGACGAATCGACATGGGTTGGAGAACATAGACGAAAATGGTGGCACCAGTATTGGAGCGGCGGGTTTATGTGCCCCGCCACCTGAAACTCCGACGGAGTCCATGGAGTTTCTGGCGAGATCATGGAGCCTCTCGGCCATGGAGATCTCCAAAGCTCTTTCCCACTCACATGTTGCTTCTAAGAGTCTTGAGAATAAGTCAGCATTCTCTTATGATGGTATTGCTGAAACTCGCCATTCAAGTTCCATGCGTTTGAGCGAATCATCTCTGCCTGGTGGACTTAGCCCTCCGATTTCGCCAAGAAACAGTGATGAAATGAAGGAGTTGTTTTTACTACATCATGCACTTCATCCAGAGTTCCTTTCCAGTCAACATCAATTGATCAATAATGGGCTATACAAGAGCATGTTGAGAGGTAAAACAATGGGGAGATGGTTAAAAGATCAAAAGGAGAGAAAGAAGCAAGAAATGAGAAGCCAAAATGCCCAGTTGCACGCGGCAGTTTCTGTGGCTGGAGTTGCTGCTGCTGTAGCAGCACTCGCAGCTTCAGCTGCATTGCCTGAAACTGAAACATCAGCTGCCAGTGCCCACCACAAAGCAGCAGTATCCGAGAGGACTGAAATTTCGACTGCACTAGCATCTGCAGCAGCTCTGGTAGCTTCACGCTGCATCGAGATTGCAGAGGAGATGGGAGCTGATCACGACCATATTCTATCAGTTGTCAGTTCTGCAGTTAACGCGAGGACTAATGGAGACATCATGACCTTAACAGCTGGAGCTGCCACAGCTTTACGAGGAGCTGCTACACTTAAGGTAAGGCTACAAAAGGAGTATGGAGGCACCACTTTTGCACTGGCTGAGGAAAAATGTGATGGAAAAGAATCAAACTCAGCATTGAACTTTGTCTCAAAAGGAGGGGAACTTCTGAAACGCACAAGGAAAGGTGATCTCCACTGGAAACAAGTTTCTTTCAACACCAACTCAAATGGGCAGGTTGTAGCCAAAATGAAAAGCAAACACATGGCGGGAACATTTACAAAGAAGAAGACATGTGTAGTCTCTGAAGTCTACTGTGACATAGCAGCGTGGCCAGGAAGGGAGAGGGAAGATTGCAGCAAACCAAGGGCATACTTTGGCATCAAAACAGCTGACAGGACAATAGAATTTGAATGCAGAAGTAAAAGGGATAAGCATATGTGGACTGAGGGGATTCAGAATATGTTGAATTTCATACCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_canalis PH_2

Protein Analysis

461

Amino Acids

49.97

Weight (kDa)

8.69

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_canalis PF05703 76 - 330 1.1e-87 Auxin canalisation
PH_2 PF08458 355 - 457 2.4e-42 Plant pleckstrin homology-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1126
AccB1I GGYRCC 2 cut(s) 188, 983
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 1212
AccII CGCG 2 cut(s) 641, 884
AciI CCGC 5 cut(s) 203, 206, 221, 641, 1169
AcoI YGGCCR 2 cut(s) 279, 1232
AcsI RAATTY 3 cut(s) 765, 1307, 1369
AcuI CTGAAG 2 cut(s) 675, 1227
AfaI GTAC 2 cut(s) 35, 48
AflII CTTAAG 1 cut(s) 953
AflIII ACRYGT 2 cut(s) 313, 1193
AgsI TTSAA 7 cut(s) 122, 139, 384, 1042, 1117, 1313, 1369
AjnI CCWGG 2 cut(s) 415, 1234
Alw26I GTCTC 3 cut(s) 891, 1054, 1207
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 2 cut(s) 279, 1232
ApoI RAATTY 3 cut(s) 765, 1307, 1369
ArsI GACNNNNNNTTYG 2 cut(s) 751, 783
AsuHPI GGTGA 2 cut(s) 53, 1100
BaeGI GKGCMC 2 cut(s) 220, 733
BalI TGGCCA 1 cut(s) 1234
BanI GGYRCC 2 cut(s) 188, 983
BarI GAAGNNNNNNTAC 2 cut(s) 39, 71
BbsI GAAGAC 1 cut(s) 1196
BbvCI CCTCAGC 1 cut(s) 1002
BccI CCATC 5 cut(s) 72, 353, 570, 826, 1010
BciT130I CCWGG 2 cut(s) 417, 1236
BciVI GTATCC 1 cut(s) 760
BclI TGATCA 2 cut(s) 525, 841
BcoDI GTCTC 3 cut(s) 891, 1054, 1207
BfaI CTAG 3 cut(s) 77, 95, 779
BfmI CTRYAG 3 cut(s) 672, 786, 873
BfrI CTTAAG 1 cut(s) 953
BfuAI ACCTGC 1 cut(s) 1126
BfuI GTATCC 1 cut(s) 760
BglII AGATCT 1 cut(s) 288
Bme1390I CCNGG 2 cut(s) 417, 1236
BmiI GGNNCC 3 cut(s) 190, 272, 985
BmrFI CCNGG 2 cut(s) 417, 1236
BmrI ACTGGG 1 cut(s) 625
BmsI GCATC 3 cut(s) 791, 822, 1290
BmuI ACTGGG 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 1196
BpmI CTGGAG 2 cut(s) 678, 939
Bpu10I CCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 353
BsaJI CCNNGG 3 cut(s) 246, 282, 1265
Bse1I ACTGG 6 cut(s) 192, 510, 631, 726, 1002, 1103
Bse3DI GCAATG 1 cut(s) 698
BseBI CCWGG 2 cut(s) 417, 1236
BseDI CCNNGG 3 cut(s) 246, 282, 1265
BseGI GGATG 1 cut(s) 493
BseMI GCAATG 1 cut(s) 698
BseMII CTCAG 3 cut(s) 993, 1047, 1338
BseNI ACTGG 6 cut(s) 192, 510, 631, 726, 1002, 1103
BseRI GAGGAG 3 cut(s) 42, 842, 954
BseSI GKGCMC 2 cut(s) 220, 733
BsgI GTGCAG 1 cut(s) 759
Bsh1236I CGCG 2 cut(s) 641, 884
BshFI GGCC 2 cut(s) 281, 1234
BshNI GGYRCC 2 cut(s) 188, 983
BsmAI GTCTC 3 cut(s) 891, 1054, 1207
BsmI GAATGC 2 cut(s) 347, 1319
BsnI GGCC 2 cut(s) 281, 1234
Bsp1286I GDGCHC 2 cut(s) 220, 733
Bsp143I GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
Bsp19I CCATGG 2 cut(s) 246, 282
BspACI CCGC 5 cut(s) 203, 206, 221, 641, 1169
BspANI GGCC 2 cut(s) 281, 1234
BspCNI CTCAG 3 cut(s) 994, 1046, 1339
BspFNI CGCG 2 cut(s) 641, 884
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 3 cut(s) 190, 272, 985
BspMAI CTGCAG 2 cut(s) 790, 877
BspMI ACCTGC 1 cut(s) 1126
BspT107I GGYRCC 2 cut(s) 188, 983
BspTI CTTAAG 1 cut(s) 953
BsrBI CCGCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 698
BsrI ACTGG 6 cut(s) 192, 510, 631, 726, 1002, 1103
BssECI CCNNGG 3 cut(s) 246, 282, 1265
BssMI GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
BssT1I CCWWGG 3 cut(s) 246, 282, 1265
Bst2UI CCWGG 2 cut(s) 417, 1236
Bst4CI ACNGT 3 cut(s) 38, 452, 1217
Bst6I CTCTTC 1 cut(s) 60
BstAFI CTTAAG 1 cut(s) 953
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 5 cut(s) 324, 425, 1002, 1033, 1347
BstDSI CCRYGG 2 cut(s) 246, 282
BstF5I GGATG 1 cut(s) 493
BstFNI CGCG 2 cut(s) 641, 884
BstKTI GATC 6 cut(s) 266, 291, 528, 589, 844, 1093
BstMAI GTCTC 3 cut(s) 891, 1054, 1207
BstMBI GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
BstNI CCWGG 2 cut(s) 417, 1236
BstNSI RCATGY 3 cut(s) 317, 553, 1197
BstSCI CCNGG 2 cut(s) 415, 1234
BstSFI CTRYAG 3 cut(s) 672, 786, 873
BstSLI GKGCMC 2 cut(s) 220, 733
BstUI CGCG 2 cut(s) 641, 884
BstV2I GAAGAC 1 cut(s) 1196
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuI GTATCC 1 cut(s) 760
BsuRI GGCC 2 cut(s) 281, 1234
BtgI CCRYGG 2 cut(s) 246, 282
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 4 cut(s) 457, 733, 995, 1096
BveI ACCTGC 1 cut(s) 1126
Cac8I GCNNGC 1 cut(s) 639
CaiI CAGNNNCTG 1 cut(s) 650
CciI TCATGA 1 cut(s) 903
Csp6I GTAC 2 cut(s) 34, 47
CviQI GTAC 2 cut(s) 34, 47
DdeI CTNAG 5 cut(s) 324, 425, 1002, 1033, 1347
DpnI GATC 6 cut(s) 265, 290, 527, 588, 843, 1092
DpnII GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
EaeI YGGCCR 2 cut(s) 279, 1232
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 3 cut(s) 246, 282, 1265
Eco57I CTGAAG 2 cut(s) 675, 1227
EcoRII CCWGG 2 cut(s) 415, 1234
EcoT14I CCWWGG 3 cut(s) 246, 282, 1265
ErhI CCWWGG 3 cut(s) 246, 282, 1265
FauI CCCGC 3 cut(s) 199, 228, 1162
FauNDI CATATG 1 cut(s) 1338
FbaI TGATCA 2 cut(s) 525, 841
FblI GTMKAC 1 cut(s) 1212
FokI GGATG 1 cut(s) 480
FspBI CTAG 3 cut(s) 77, 95, 779
GsuI CTGGAG 2 cut(s) 678, 939
HaeIII GGCC 2 cut(s) 281, 1234
HincII GTYRAC 2 cut(s) 515, 880
HindII GTYRAC 2 cut(s) 515, 880
HinfI GANTC 7 cut(s) 98, 151, 242, 328, 404, 1025, 1354
HpaI GTTAAC 1 cut(s) 880
HphI GGTGA 2 cut(s) 53, 1100
Hpy166II GTNNAC 5 cut(s) 422, 515, 880, 1213, 1344
Hpy188I TCNGA 6 cut(s) 237, 435, 754, 1071, 1207, 1359
Hpy188III TCNNGA 5 cut(s) 332, 497, 817, 845, 904
Hpy8I GTNNAC 5 cut(s) 422, 515, 880, 1213, 1344
Hpy99I CGWCG 2 cut(s) 151, 241
HpyAV CCTTC 2 cut(s) 457, 1233
HpyCH4III ACNGT 3 cut(s) 38, 452, 1217
HpyF3I CTNAG 5 cut(s) 324, 425, 1002, 1033, 1347
Ksp22I TGATCA 2 cut(s) 525, 841
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
LmnI GCTCC 5 cut(s) 200, 270, 836, 920, 941
LweI GCATC 3 cut(s) 791, 822, 1290
MaeI CTAG 3 cut(s) 77, 95, 779
MaeIII GTNAC 1 cut(s) 1217
MalI GATC 6 cut(s) 265, 290, 527, 588, 843, 1092
MbiI CCGCTC 1 cut(s) 203
MboI GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
MboII GAAGA 4 cut(s) 47, 1198, 1201, 1262
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 2 cut(s) 220, 733
MlsI TGGCCA 1 cut(s) 1234
MluCI AATT 4 cut(s) 521, 765, 1307, 1369
MluNI TGGCCA 1 cut(s) 1234
MlyI GAGTC 2 cut(s) 251, 337
MmeI TCCRAC 2 cut(s) 144, 260
Mox20I TGGCCA 1 cut(s) 1234
MscI TGGCCA 1 cut(s) 1234
MseI TTAA 5 cut(s) 26, 581, 879, 911, 954
MslI CAYNNNNRTG 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 1234
MspA1I CMGCKG 4 cut(s) 695, 722, 917, 1292
MspCI CTTAAG 1 cut(s) 953
MspR9I CCNGG 2 cut(s) 417, 1236
MunI CAATTG 1 cut(s) 521
Mva1269I GAATGC 2 cut(s) 347, 1319
MvaI CCWGG 2 cut(s) 417, 1236
MvnI CGCG 2 cut(s) 641, 884
NcoI CCATGG 2 cut(s) 246, 282
NdeI CATATG 1 cut(s) 1338
NdeII GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
NlaIV GGNNCC 3 cut(s) 190, 272, 985
NmeAIII GCCGAG 2 cut(s) 139, 257
NmuCI GTSAC 1 cut(s) 1217
NspI RCATGY 3 cut(s) 317, 553, 1197
PagI TCATGA 1 cut(s) 903
PciI ACATGT 2 cut(s) 313, 1193
PctI GAATGC 2 cut(s) 347, 1319
PfeI GAWTC 5 cut(s) 98, 151, 404, 1025, 1354
PflFI GACNNNGTC 1 cut(s) 241
PleI GAGTC 2 cut(s) 250, 336
PpsI GAGTC 2 cut(s) 250, 336
PscI ACATGT 2 cut(s) 313, 1193
Psp6I CCWGG 2 cut(s) 415, 1234
PspGI CCWGG 2 cut(s) 415, 1234
PspN4I GGNNCC 3 cut(s) 190, 272, 985
PstI CTGCAG 2 cut(s) 790, 877
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 288
PsyI GACNNNGTC 1 cut(s) 241
PvuII CAGCTG 4 cut(s) 695, 722, 917, 1292
RsaI GTAC 2 cut(s) 35, 48
RsaNI GTAC 2 cut(s) 34, 47
RseI CAYNNNNRTG 1 cut(s) 312
SaqAI TTAA 5 cut(s) 26, 581, 879, 911, 954
Sau3AI GATC 6 cut(s) 263, 288, 525, 586, 841, 1090
SchI GAGTC 2 cut(s) 251, 337
ScrFI CCNGG 2 cut(s) 417, 1236
SduI GDGCHC 2 cut(s) 220, 733
SfaNI GCATC 3 cut(s) 791, 822, 1290
SfcI CTRYAG 3 cut(s) 672, 786, 873
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 332, 953
SmoI CTYRAG 2 cut(s) 332, 953
Sse9I AATT 4 cut(s) 521, 765, 1307, 1369
SsiI CCGC 5 cut(s) 203, 206, 221, 641, 1169
SspMI CTAG 3 cut(s) 77, 95, 779
StyD4I CCNGG 2 cut(s) 415, 1234
StyI CCWWGG 3 cut(s) 246, 282, 1265
TaaI ACNGT 3 cut(s) 38, 452, 1217
TaqI TCGA 4 cut(s) 146, 154, 770, 816
TasI AATT 4 cut(s) 521, 765, 1307, 1369
TauI GCSGC 2 cut(s) 206, 644
TfiI GAWTC 5 cut(s) 98, 151, 404, 1025, 1354
Tru1I TTAA 5 cut(s) 26, 581, 879, 911, 954
Tru9I TTAA 5 cut(s) 26, 581, 879, 911, 954
TscAI CASTG 4 cut(s) 457, 733, 1002, 1103
TseFI GTSAC 1 cut(s) 1217
Tsp45I GTSAC 1 cut(s) 1217
TspDTI ATGAA 6 cut(s) 47, 471, 476, 482, 1166, 1363
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 4 cut(s) 457, 733, 1002, 1103
Tth111I GACNNNGTC 1 cut(s) 241
Vha464I CTTAAG 1 cut(s) 953
XapI RAATTY 3 cut(s) 765, 1307, 1369
XceI RCATGY 3 cut(s) 317, 553, 1197
XcmI CCANNNNNNNNNTGG 2 cut(s) 253, 994
XmiI GTMKAC 1 cut(s) 1212
XspI CTAG 3 cut(s) 77, 95, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.