AT4G35230

serine threonine-protein kinase At4g35230

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003677
Physical Location & Seq
Reverse (-)
16755092 .. 16758358
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35230.1

Sequence Viewer

Length: 1539 bp
ATGGGTTGTTGTCAATCCTTGTTTTCCGGCGATAACCCACTTGGGAAAGATGGAGTTCAGCCGCAACCACTATCTCAAAACAATCACGGCGGAGCTACGACGGCTGATAACGGCGGAAGCGGCGGAGCTAGTGGTGTAGGCGGCGGAGGAGGAGGAGGAGGAATCCCATCTTTCTCTGAGTTCTCTTTCGCTGACCTTAAAGCAGCTACAAACAACTTTAGCTCAGACAACATCGTATCAGAAAGTGGTGAGAAAGCCCCAAATCTTGTCTACAAAGGCCGACTTCAGAACCGTCGTTGGATCGCTGTCAAGAAGTTTACTAAGATGGCTTGGCCTGAGCCTAAACAATTTGCGGAAGAAGCATGGGGTGTGGGGAAATTGAGGCATAATCGATTAGCGAATTTGATTGGATATTGTTGTGATGGAGATGAGAGGCTTCTTGTTGCTGAGTTCATGCCTAATGATACTCTTGCTAAGCATTTGTTCCATTGGGAAAATCAGACAATTGAGTGGGCTATGAGGTTGCGAGTAGGATATTACATAGCTGAGGCTTTGGATTATTGTAGTACTGAGGGTCGTCCATTGTACCATGATTTGAATGCTTATAGGGTTCTCTTTGATGAGGATGGTGATCCTCGTCTCTCATGTTTTGGCTTGATGAAGAACAGTAGGGATGGTAAAAGTTATAGCACAAATTTAGCTTATACACCACCTGAATATCTAAGAAATGGAAGAGTGACACCTGAAAGTGTTACGTATAGCTTTGGAACTGTCCTTCTGGATTTGCTTAGCGGAAAACACATCCCTCCAAGCCATGCTCTCGATATGATACGAGGCAAGAATATTATTCTGTTGATGGATTCACACCTCGAAGGAAAGTTCTCAACAGAAGAGGCTACTGTAGTGGTCGAACTCGCCTCTCAATGTTTACAATATGAGCCTCGAGAGAGACCAAATACAAAAGATCTTGTTGCAACACTTGCACCATTGCAAACTAAATCAGACGTTCCATCTTATGTGATGCTTGGAATAAAGAAGCAAGAGGAGGCACCTTCGACTCCACAGAGACCACTTTCGCCATTAGGTGAGGCCTGCTCAAGAATGGATCTCACAGCTATTCATCAGATTTTGGTCATGACACATTACAGAGACGACGAGGGCACAAACGAGTTATCATTCCAAGAATGGACTCAACAAATGAAAGATATGCTTGATGCACGGAAACGCGGTGATCAATCTTTCCGCGAGAAAGATTTCAAAACAGCCATCGACTGTTACTCACAGTTCATTGACGTTGGAACAATGGTATCTCCAACTGTATTTGGTCGGCGAAGTCTATGCTACTTACTATGCGATCAACCAGATGCAGCACTACGCGACGCAATGCAAGCGCAGTGTGTGTATCCGGATTGGCCAACAGCTTTCTACATGCAGTCTGTGGCATTAGCGAAGCTGAACATGAACACAGACGCAGCCGATATGTTGAACGAAGCAGCTCAGCTCGAAGAAAAGAGACAACGAGGCGGCAGAGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009741 GO:0009742 GO:0009755 GO:0009790 GO:0009826 GO:0009898 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010098 GO:0010646 GO:0010647 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0030054 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048518 GO:0048522 GO:0048545 GO:0048583 GO:0048584 GO:0048589 GO:0048598 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060089 GO:0060560 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0080159 GO:0097305 GO:0098542 GO:0098552 GO:0098562 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

512

Amino Acids

56.82

Weight (kDa)

5.52

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 90 - 327 1e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 90 - 325 1.9e-22 Protein kinase domain
TPR_BSK1_C PF25575 363 - 479 3.7e-61 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1048
AccI GTMKAC 1 cut(s) 270
AccII CGCG 3 cut(s) 1227, 1245, 1377
AccIII TCCGGA 1 cut(s) 1405
AclWI GGATC 3 cut(s) 308, 626, 1113
AcoI YGGCCR 1 cut(s) 1412
AcsI RAATTY 2 cut(s) 400, 694
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 2 cut(s) 568, 587
AgsI TTSAA 3 cut(s) 598, 1258, 1486
Alw26I GTCTC 5 cut(s) 644, 943, 1060, 1143, 1507
AlwI GGATC 3 cut(s) 308, 626, 1113
Ama87I CYCGRG 1 cut(s) 942
Aor13HI TCCGGA 1 cut(s) 1405
AoxI GGCC 4 cut(s) 277, 332, 1089, 1412
ApeKI GCWGC 4 cut(s) 203, 1367, 1472, 1493
ApoI RAATTY 2 cut(s) 400, 694
Asp700I GAANNNNTTC 1 cut(s) 1253
AspLEI GCGC 1 cut(s) 1393
AsuHPI GGTGA 4 cut(s) 260, 641, 1097, 1241
AvaI CYCGRG 1 cut(s) 942
BaeGI GKGCMC 1 cut(s) 1163
BaeI ACNNNNGTAYC 2 cut(s) 1290, 1323
BalI TGGCCA 1 cut(s) 1414
BanI GGYRCC 1 cut(s) 1048
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 4 cut(s) 215, 1379, 1484, 1505
BccI CCATC 9 cut(s) 44, 175, 319, 416, 620, 668, 850, 1018, 1274
BceAI ACGGC 3 cut(s) 103, 117, 127
BcgI CGANNNNNNTGC 2 cut(s) 1320, 1354
BciVI GTATCC 1 cut(s) 1413
BclI TGATCA 1 cut(s) 1231
BcoDI GTCTC 5 cut(s) 644, 943, 1060, 1143, 1507
BfaI CTAG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 900
BfuI GTATCC 1 cut(s) 1413
BglII AGATCT 1 cut(s) 964
BisI GCNGC 8 cut(s) 62, 121, 142, 204, 1368, 1473, 1494, 1525
BlpI GCTNAGC 3 cut(s) 474, 788, 1497
BlsI GCNGC 8 cut(s) 63, 122, 143, 205, 1369, 1474, 1495, 1526
BmcAI AGTACT 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 942
BmiI GGNNCC 1 cut(s) 1050
BmsI GCATC 3 cut(s) 1011, 1204, 1354
BplI GAGNNNNNCTC 2 cut(s) 1079, 1111
Bpu10I CCTNAGC 2 cut(s) 336, 546
Bpu1102I GCTNAGC 3 cut(s) 474, 788, 1497
BpuEI CTTGAG 1 cut(s) 1081
Bsa29I ATCGAT 1 cut(s) 391
BsaAI YACGTR 1 cut(s) 756
BsaBI GATNNNNATC 1 cut(s) 630
BsaI GGTCTC 2 cut(s) 943, 1060
BsaWI WCCGGW 1 cut(s) 1405
Bse3DI GCAATG 2 cut(s) 986, 1389
Bse8I GATNNNNATC 1 cut(s) 630
BseAI TCCGGA 1 cut(s) 1405
BseCI ATCGAT 1 cut(s) 391
BseGI GGATG 3 cut(s) 631, 679, 801
BseJI GATNNNNATC 1 cut(s) 630
BseMI GCAATG 2 cut(s) 986, 1389
BseMII CTCAG 7 cut(s) 168, 237, 327, 438, 537, 561, 1511
BseRI GAGGAG 5 cut(s) 162, 165, 168, 171, 1058
BseSI GKGCMC 1 cut(s) 1163
BseXI GCAGC 4 cut(s) 215, 1379, 1484, 1505
Bsh1236I CGCG 3 cut(s) 1227, 1245, 1377
BshFI GGCC 4 cut(s) 279, 334, 1091, 1414
BshNI GGYRCC 1 cut(s) 1048
BshVI ATCGAT 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 942
BsiSI CCGG 2 cut(s) 27, 1406
BsmAI GTCTC 5 cut(s) 644, 943, 1060, 1143, 1507
BsmBI CGTCTC 2 cut(s) 644, 1143
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 4 cut(s) 279, 334, 1091, 1414
Bso31I GGTCTC 2 cut(s) 943, 1060
BsoBI CYCGRG 1 cut(s) 942
Bsp1286I GDGCHC 1 cut(s) 1163
Bsp13I TCCGGA 1 cut(s) 1405
Bsp143I GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
Bsp1720I GCTNAGC 3 cut(s) 474, 788, 1497
BspANI GGCC 4 cut(s) 279, 334, 1091, 1414
BspCNI CTCAG 7 cut(s) 169, 236, 328, 439, 538, 562, 1510
BspDI ATCGAT 1 cut(s) 391
BspEI TCCGGA 1 cut(s) 1405
BspFNI CGCG 3 cut(s) 1227, 1245, 1377
BspHI TCATGA 1 cut(s) 1134
BspLI GGNNCC 1 cut(s) 1050
BspPI GGATC 3 cut(s) 308, 626, 1113
BspT107I GGYRCC 1 cut(s) 1048
BspTNI GGTCTC 2 cut(s) 943, 1060
BsrDI GCAATG 2 cut(s) 986, 1389
BssMI GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
Bst4CI ACNGT 7 cut(s) 293, 668, 772, 901, 1274, 1284, 1318
Bst6I CTCTTC 2 cut(s) 727, 885
BstAPI GCANNNNNTGC 1 cut(s) 980
BstBAI YACGTR 1 cut(s) 756
BstC8I GCNNGC 2 cut(s) 1093, 1389
BstF5I GGATG 3 cut(s) 631, 679, 801
BstFNI CGCG 3 cut(s) 1227, 1245, 1377
BstHHI GCGC 1 cut(s) 1393
BstKTI GATC 7 cut(s) 303, 634, 967, 1108, 1234, 1357, 1535
BstMAI GTCTC 5 cut(s) 644, 943, 1060, 1143, 1507
BstMBI GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
BstMWI GCNNNNNNNGC 5 cut(s) 101, 120, 359, 980, 1388
BstNSI RCATGY 1 cut(s) 1432
BstSFI CTRYAG 1 cut(s) 900
BstSLI GKGCMC 1 cut(s) 1163
BstSNI TACGTA 1 cut(s) 756
BstUI CGCG 3 cut(s) 1227, 1245, 1377
BstV1I GCAGC 4 cut(s) 215, 1379, 1484, 1505
BstX2I RGATCY 3 cut(s) 964, 1105, 1532
BstYI RGATCY 3 cut(s) 964, 1105, 1532
Bsu15I ATCGAT 1 cut(s) 391
BsuI GTATCC 1 cut(s) 1413
BsuRI GGCC 4 cut(s) 279, 334, 1091, 1414
BsuTUI ATCGAT 1 cut(s) 391
BtsCI GGATG 3 cut(s) 631, 679, 801
BtsI GCAGTG 1 cut(s) 1400
BtsIMutI CAGTG 1 cut(s) 1400
Cac8I GCNNGC 2 cut(s) 1093, 1389
CciI TCATGA 1 cut(s) 1134
CfoI GCGC 1 cut(s) 1393
ClaI ATCGAT 1 cut(s) 391
CseI GACGC 2 cut(s) 1388, 1478
Csp6I GTAC 2 cut(s) 567, 586
CviAII CATG 8 cut(s) 363, 454, 590, 645, 815, 1135, 1429, 1459
CviQI GTAC 2 cut(s) 567, 586
DpnI GATC 7 cut(s) 302, 633, 966, 1107, 1233, 1356, 1534
DpnII GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
EaeI YGGCCR 1 cut(s) 1412
Eam1104I CTCTTC 2 cut(s) 727, 885
EarI CTCTTC 2 cut(s) 727, 885
EciI GGCGGA 4 cut(s) 105, 129, 138, 159
Eco105I TACGTA 1 cut(s) 756
Eco147I AGGCCT 1 cut(s) 1091
Eco31I GGTCTC 2 cut(s) 943, 1060
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 942
Esp3I CGTCTC 2 cut(s) 644, 1143
FaeI CATG 8 cut(s) 366, 457, 593, 648, 818, 1138, 1432, 1462
FalI AAGNNNNNCTT 4 cut(s) 267, 299, 1194, 1226
FatI CATG 8 cut(s) 362, 453, 589, 644, 814, 1134, 1428, 1458
FbaI TGATCA 1 cut(s) 1231
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 8 cut(s) 62, 121, 142, 204, 1368, 1473, 1494, 1525
FokI GGATG 3 cut(s) 638, 686, 788
Fsp4HI GCNGC 8 cut(s) 62, 121, 142, 204, 1368, 1473, 1494, 1525
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 1392
GluI GCNGC 8 cut(s) 62, 121, 142, 204, 1368, 1473, 1494, 1525
HaeIII GGCC 4 cut(s) 279, 334, 1091, 1414
HapII CCGG 2 cut(s) 27, 1406
HgaI GACGC 2 cut(s) 1388, 1478
HhaI GCGC 1 cut(s) 1393
Hin1II CATG 8 cut(s) 366, 457, 593, 648, 818, 1138, 1432, 1462
Hin6I GCGC 1 cut(s) 1391
HinP1I GCGC 1 cut(s) 1391
HinfI GANTC 4 cut(s) 162, 860, 1057, 1189
HpaII CCGG 2 cut(s) 27, 1406
HphI GGTGA 4 cut(s) 260, 641, 1097, 1241
Hpy166II GTNNAC 3 cut(s) 271, 318, 929
Hpy188I TCNGA 7 cut(s) 178, 226, 241, 288, 501, 1003, 1125
Hpy188III TCNNGA 8 cut(s) 310, 779, 821, 944, 1098, 1135, 1406, 1536
Hpy8I GTNNAC 3 cut(s) 271, 318, 929
Hpy99I CGWCG 4 cut(s) 103, 297, 1157, 1382
HpyAV CCTTC 3 cut(s) 785, 866, 1062
HpyCH4III ACNGT 7 cut(s) 293, 668, 772, 901, 1274, 1284, 1318
HpyCH4IV ACGT 3 cut(s) 755, 1005, 1293
HpyCH4V TGCA 7 cut(s) 974, 983, 991, 1217, 1367, 1387, 1432
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 120, 359, 980, 1388
HpySE526I ACGT 3 cut(s) 755, 1005, 1293
Hsp92II CATG 8 cut(s) 366, 457, 593, 648, 818, 1138, 1432, 1462
HspAI GCGC 1 cut(s) 1391
Kpn2I TCCGGA 1 cut(s) 1405
Ksp22I TGATCA 1 cut(s) 1231
Kzo9I GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
LmnI GCTCC 2 cut(s) 92, 125
LpnPI CCDG 8 cut(s) 40, 348, 726, 756, 764, 1105, 1374, 1419
Lsp1109I GCAGC 4 cut(s) 215, 1379, 1484, 1505
LweI GCATC 3 cut(s) 1011, 1204, 1354
MaeI CTAG 1 cut(s) 129
MaeII ACGT 3 cut(s) 755, 1005, 1293
MaeIII GTNAC 3 cut(s) 736, 751, 1274
MalI GATC 7 cut(s) 302, 633, 966, 1107, 1233, 1356, 1534
MboI GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
MboII GAAGA 5 cut(s) 368, 673, 744, 902, 1517
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 3 cut(s) 964, 1105, 1532
MhlI GDGCHC 1 cut(s) 1163
MlsI TGGCCA 1 cut(s) 1414
MluCI AATT 5 cut(s) 347, 377, 400, 504, 694
MluNI TGGCCA 1 cut(s) 1414
MlyI GAGTC 2 cut(s) 1051, 1183
MmeI TCCRAC 3 cut(s) 278, 1276, 1337
Mox20I TGGCCA 1 cut(s) 1414
MroI TCCGGA 1 cut(s) 1405
MroXI GAANNNNTTC 1 cut(s) 1253
MscI TGGCCA 1 cut(s) 1414
MseI TTAA 1 cut(s) 198
Msp20I TGGCCA 1 cut(s) 1414
MspI CCGG 2 cut(s) 27, 1406
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 604
MvnI CGCG 3 cut(s) 1227, 1245, 1377
MwoI GCNNNNNNNGC 5 cut(s) 101, 120, 359, 980, 1388
NdeII GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
NlaIII CATG 8 cut(s) 366, 457, 593, 648, 818, 1138, 1432, 1462
NlaIV GGNNCC 1 cut(s) 1050
NmuCI GTSAC 1 cut(s) 736
NspI RCATGY 1 cut(s) 1432
PaeR7I CTCGAG 1 cut(s) 942
PagI TCATGA 1 cut(s) 1134
PceI AGGCCT 1 cut(s) 1091
PctI GAATGC 1 cut(s) 604
PdmI GAANNNNTTC 1 cut(s) 1253
PfeI GAWTC 2 cut(s) 162, 860
PkrI GCNGC 8 cut(s) 63, 122, 143, 205, 1369, 1474, 1495, 1526
PleI GAGTC 2 cut(s) 1051, 1183
PpsI GAGTC 2 cut(s) 1051, 1183
Ppu21I YACGTR 1 cut(s) 756
PspN4I GGNNCC 1 cut(s) 1050
PsuI RGATCY 3 cut(s) 964, 1105, 1532
RsaI GTAC 2 cut(s) 568, 587
RsaNI GTAC 2 cut(s) 567, 586
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 8 cut(s) 62, 121, 142, 204, 1368, 1473, 1494, 1525
Sau3AI GATC 7 cut(s) 300, 631, 964, 1105, 1231, 1354, 1532
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 2 cut(s) 1051, 1183
SduI GDGCHC 1 cut(s) 1163
SfaNI GCATC 3 cut(s) 1011, 1204, 1354
SfcI CTRYAG 1 cut(s) 900
Sfr274I CTCGAG 1 cut(s) 942
SlaI CTCGAG 1 cut(s) 942
SmlI CTYRAG 2 cut(s) 942, 1096
SmoI CTYRAG 2 cut(s) 942, 1096
SnaBI TACGTA 1 cut(s) 756
Sse9I AATT 5 cut(s) 347, 377, 400, 504, 694
SseBI AGGCCT 1 cut(s) 1091
SspI AATATT 1 cut(s) 844
SspMI CTAG 1 cut(s) 129
StuI AGGCCT 1 cut(s) 1091
TaaI ACNGT 7 cut(s) 293, 668, 772, 901, 1274, 1284, 1318
TaiI ACGT 3 cut(s) 758, 1008, 1296
TaqI TCGA 8 cut(s) 391, 822, 870, 909, 943, 1055, 1269, 1503
TasI AATT 5 cut(s) 347, 377, 400, 504, 694
TatI WGTACW 1 cut(s) 566
TauI GCSGC 4 cut(s) 64, 123, 144, 1527
TfiI GAWTC 2 cut(s) 162, 860
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 1 cut(s) 1400
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 4 cut(s) 203, 1367, 1472, 1493
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 6 cut(s) 442, 674, 1109, 1214, 1276, 1475
TspGWI ACGGA 1 cut(s) 1234
TspRI CASTG 1 cut(s) 1400
XapI RAATTY 2 cut(s) 400, 694
XceI RCATGY 1 cut(s) 1432
XhoI CTCGAG 1 cut(s) 942
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 1253
XspI CTAG 1 cut(s) 129
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.