pycom05g12830

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003677
Physical Location & Seq
Reverse (-)
16346363 .. 16347812
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12830.1

Sequence Viewer

Length: 684 bp
ATGGATTCACACTTGGAGGGGAAATTTTCTACAGAGGAAGCAACTGTAGTTGTTAATCTTGCCTCTCAATGTCTCCAATATGAACCTAGAGAGCGGCCTAATACAAAGGACCTTGTTGCAACACTCGCTCCACTGCAGACCAAACCTGATGTTCCATCTTATGTCATGCTTGGAATTCCAAAGCATGAAGAAGCGCCTTCAACCCCACAACGTCCCCTTTCTGCTATGGGTGACGCCTGCTCTCGGATGGACCTCACAGCAATCCATCAGATCTTGGTTATGACGCACTACAGAGATGATGAAGGAACCAATGAGTTATCTTTCCAAGAGTGGACTCAACAGATGAGAGACATGTTGGAGGCAAGGAAACGAGGGGATTATGCATTTCGGGACAAAGATTTTAAAGCAGCCATTGAGTGTTACTCCCAGTTCATAGATGTTGGAACTATGGTTTCGCCAACTGTTTTTGCCCGACGCAGTTTATGCTACCTGTTAAATGAGCAACCAGATGCTGCCCTTCGAGATGCAATGCAAGCGCAATGTGTCTATCCGGAATGGCCCACAGCCTTCTACATGCAGTCAGTTGCTCTAGCCAAGCTGGACATGCACAAGGATGCTGCTGACATGTTAAATGAAGCGACTGCGCTGGAAGAAAAGAAGCAGCGAGGAGGCCGGGGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

228

Amino Acids

25.66

Weight (kDa)

5.09

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 78 - 194 7.5e-62 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 94
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 1 cut(s) 94
AcsI RAATTY 2 cut(s) 23, 174
AcyI GRCGYC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 243
AflIII ACRYGT 2 cut(s) 351, 624
AgsI TTSAA 1 cut(s) 201
AluBI AGCT 1 cut(s) 598
AluI AGCT 1 cut(s) 598
Alw26I GTCTC 2 cut(s) 77, 342
AlwNI CAGNNNCTG 1 cut(s) 512
Aor13HI TCCGGA 1 cut(s) 550
AoxI GGCC 3 cut(s) 95, 557, 670
ApeKI GCWGC 4 cut(s) 407, 512, 617, 661
ApoI RAATTY 2 cut(s) 23, 174
AspLEI GCGC 3 cut(s) 196, 538, 646
AspS9I GGNCC 3 cut(s) 109, 250, 558
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 1 cut(s) 242
AvaII GGWCC 2 cut(s) 109, 250
BbvI GCAGC 4 cut(s) 419, 499, 604, 673
BccI CCATC 3 cut(s) 163, 241, 273
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 2 cut(s) 77, 342
BfaI CTAG 2 cut(s) 87, 590
BfmI CTRYAG 4 cut(s) 30, 45, 134, 289
BfoI RGCGCY 1 cut(s) 197
BglII AGATCT 1 cut(s) 270
BisI GCNGC 5 cut(s) 95, 408, 513, 618, 662
BlsI GCNGC 5 cut(s) 96, 409, 514, 619, 663
Bme1390I CCNGG 1 cut(s) 674
Bme18I GGWCC 2 cut(s) 109, 250
BmgT120I GGNCC 3 cut(s) 109, 250, 558
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 1 cut(s) 674
BmrI ACTGGG 1 cut(s) 421
BmsI GCATC 3 cut(s) 499, 514, 604
BmuI ACTGGG 1 cut(s) 421
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpuMI CCSGG 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 673
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 427
Bse3DI GCAATG 2 cut(s) 534, 545
BseAI TCCGGA 1 cut(s) 550
BseDI CCNNGG 1 cut(s) 673
BseGI GGATG 2 cut(s) 252, 619
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMI GCAATG 2 cut(s) 534, 545
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 1 cut(s) 681
BseXI GCAGC 4 cut(s) 419, 499, 604, 673
BshFI GGCC 3 cut(s) 97, 559, 672
BsiSI CCGG 2 cut(s) 551, 673
BslFI GGGAC 2 cut(s) 198, 404
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 2 cut(s) 77, 342
BsmFI GGGAC 2 cut(s) 198, 404
BsnI GGCC 3 cut(s) 97, 559, 672
Bsp13I TCCGGA 1 cut(s) 550
Bsp143I GATC 2 cut(s) 270, 677
BspACI CCGC 1 cut(s) 94
BspANI GGCC 3 cut(s) 97, 559, 672
BspEI TCCGGA 1 cut(s) 550
BspHI TCATGA 1 cut(s) 680
BspLI GGNNCC 1 cut(s) 307
BspMAI CTGCAG 1 cut(s) 138
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 534, 545
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 673
BssMI GATC 2 cut(s) 270, 677
BssNI GRCGYC 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 46, 463
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 483
BstC8I GCNNGC 2 cut(s) 238, 534
BstF5I GGATG 2 cut(s) 252, 619
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 3 cut(s) 196, 538, 646
BstKTI GATC 2 cut(s) 273, 680
BstMAI GTCTC 2 cut(s) 77, 342
BstMBI GATC 2 cut(s) 270, 677
BstMWI GCNNNNNNNGC 4 cut(s) 125, 483, 533, 604
BstNSI RCATGY 4 cut(s) 355, 577, 607, 628
BstSCI CCNGG 1 cut(s) 672
BstSFI CTRYAG 4 cut(s) 30, 45, 134, 289
BstV1I GCAGC 4 cut(s) 419, 499, 604, 673
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 3 cut(s) 97, 559, 672
BtsCI GGATG 2 cut(s) 252, 619
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 2 cut(s) 238, 534
CaiI CAGNNNCTG 1 cut(s) 512
CciI TCATGA 1 cut(s) 680
CfoI GCGC 3 cut(s) 196, 538, 646
Cfr13I GGNCC 3 cut(s) 109, 250, 558
CseI GACGC 3 cut(s) 242, 292, 483
CviAII CATG 7 cut(s) 166, 185, 352, 574, 604, 625, 681
CviJI RGCY 7 cut(s) 97, 410, 559, 566, 593, 598, 672
CviKI_1 RGCY 7 cut(s) 97, 410, 559, 566, 593, 598, 672
DpnI GATC 2 cut(s) 272, 679
DpnII GATC 2 cut(s) 270, 677
DraI TTTAAA 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 109, 250
EcoO109I RGGNCCY 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 174
EcoT22I ATGCAT 1 cut(s) 385
FaeI CATG 7 cut(s) 169, 188, 355, 577, 607, 628, 684
FaqI GGGAC 2 cut(s) 198, 404
FatI CATG 7 cut(s) 165, 184, 351, 573, 603, 624, 680
Fnu4HI GCNGC 5 cut(s) 95, 408, 513, 618, 662
FokI GGATG 2 cut(s) 259, 626
Fsp4HI GCNGC 5 cut(s) 95, 408, 513, 618, 662
FspBI CTAG 2 cut(s) 87, 590
GlaI GCGC 3 cut(s) 195, 537, 645
GluI GCNGC 5 cut(s) 95, 408, 513, 618, 662
HaeII RGCGCY 1 cut(s) 197
HaeIII GGCC 3 cut(s) 97, 559, 672
HapII CCGG 2 cut(s) 551, 673
HgaI GACGC 3 cut(s) 242, 292, 483
HhaI GCGC 3 cut(s) 196, 538, 646
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 7 cut(s) 169, 188, 355, 577, 607, 628, 684
Hin6I GCGC 3 cut(s) 194, 536, 644
HinP1I GCGC 3 cut(s) 194, 536, 644
HinfI GANTC 2 cut(s) 5, 334
HpaII CCGG 2 cut(s) 551, 673
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 2 cut(s) 246, 270
Hpy188III TCNNGA 4 cut(s) 389, 521, 551, 681
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 477
HpyAV CCTTC 4 cut(s) 207, 296, 527, 577
HpyCH4III ACNGT 2 cut(s) 46, 463
HpyCH4IV ACGT 1 cut(s) 211
HpyCH4V TGCA 7 cut(s) 119, 136, 383, 527, 532, 577, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 483, 533, 604
HpySE526I ACGT 1 cut(s) 211
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 7 cut(s) 169, 188, 355, 577, 607, 628, 684
HspAI GCGC 3 cut(s) 194, 536, 644
Kpn2I TCCGGA 1 cut(s) 550
Kzo9I GATC 2 cut(s) 270, 677
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 8 cut(s) 159, 250, 440, 503, 519, 564, 584, 632
Lsp1109I GCAGC 4 cut(s) 419, 499, 604, 673
LweI GCATC 3 cut(s) 499, 514, 604
MaeI CTAG 2 cut(s) 87, 590
MaeII ACGT 1 cut(s) 211
MaeIII GTNAC 2 cut(s) 230, 419
MalI GATC 2 cut(s) 272, 679
MbiI CCGCTC 1 cut(s) 94
MboI GATC 2 cut(s) 270, 677
MboII GAAGA 2 cut(s) 200, 662
MflI RGATCY 1 cut(s) 270
MluCI AATT 2 cut(s) 23, 174
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 2 cut(s) 336, 421
MnlI CCTC 8 cut(s) 10, 28, 73, 263, 352, 365, 659, 662
Mph1103I ATGCAT 1 cut(s) 385
MroI TCCGGA 1 cut(s) 550
MseI TTAA 4 cut(s) 54, 402, 494, 629
MslI CAYNNNNRTG 1 cut(s) 612
MspI CCGG 2 cut(s) 551, 673
MspR9I CCNGG 1 cut(s) 674
MwoI GCNNNNNNNGC 4 cut(s) 125, 483, 533, 604
NciI CCSGG 1 cut(s) 674
NdeII GATC 2 cut(s) 270, 677
NlaIII CATG 7 cut(s) 169, 188, 355, 577, 607, 628, 684
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 230
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 4 cut(s) 355, 577, 607, 628
PagI TCATGA 1 cut(s) 680
PciI ACATGT 2 cut(s) 351, 624
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 5 cut(s) 96, 409, 514, 619, 663
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 109
PscI ACATGT 2 cut(s) 351, 624
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 3 cut(s) 109, 250, 558
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 270
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 4 cut(s) 54, 402, 494, 629
SatI GCNGC 5 cut(s) 95, 408, 513, 618, 662
Sau3AI GATC 2 cut(s) 270, 677
Sau96I GGNCC 3 cut(s) 109, 250, 558
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 674
SetI ASST 7 cut(s) 88, 114, 148, 214, 255, 492, 600
SfaNI GCATC 3 cut(s) 499, 514, 604
SfcI CTRYAG 4 cut(s) 30, 45, 134, 289
SinI GGWCC 2 cut(s) 109, 250
SmiMI CAYNNNNRTG 1 cut(s) 612
Sse9I AATT 2 cut(s) 23, 174
SsiI CCGC 1 cut(s) 94
SspMI CTAG 2 cut(s) 87, 590
StyD4I CCNGG 1 cut(s) 672
TaaI ACNGT 2 cut(s) 46, 463
TaiI ACGT 1 cut(s) 214
TaqI TCGA 1 cut(s) 520
TasI AATT 2 cut(s) 23, 174
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 4 cut(s) 54, 402, 494, 629
Tru9I TTAA 4 cut(s) 54, 402, 494, 629
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 4 cut(s) 407, 512, 617, 661
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 5 cut(s) 96, 201, 315, 421, 648
TspRI CASTG 1 cut(s) 138
VpaK11BI GGWCC 2 cut(s) 109, 250
XapI RAATTY 2 cut(s) 23, 174
XceI RCATGY 4 cut(s) 355, 577, 607, 628
XspI CTAG 2 cut(s) 87, 590
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.