Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003677

Physical Location & Seq

Genomic Coordinates Forward (+)

368620 .. 372850

Gene Structure 4231 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1003600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1533 bp

ATGGGGTGCTGCGAGTCGTCTTTTATACATGCACGCCAACACCAGCACCACCCCTTGAACACCTTCAGCTCCACCGCCACCGCCACCACCGCAACCACCAACCGCGTTCCTCAACCGTCCAACGGTGGAACCGATGCCGCCGGAGCTGGAGCAGTGCCGGGATTTTCGGAATTCTCGCTGGCCGACTTGAAAAGCGCCACAAATAACTTCAGCTCCGACTTCATCGTCTCCGAGAGCGGCGAGAAAGCCCCCAATGTCGTCTACAAGGGACGCCTCCAGAACCAAAATAACCGTCGTTGGATTGCCGTCAAGAAATTTTCCAAATTGGCCTGGCCTGACCCCAAGCAATTCGCCGATGAAGCTTGGGGCGTTGGGAAGCTGAGGCACAAAAGGCTTGCCAATTTGATAGGGTATTGCTGCGACGGCGACGAGAGGCTGCTGGTGGCGGAGTACATGCCTAATGATACCCTTGCCAAACATCTATTCCACTGGGAGAATCAGACCATTGAGTGGGCTATGCGACTAAGAGTTGCTCTGAACATTGCCGAAGCATTGAATTACTGCAGTAGTGAAGGCCGCCCATTGTACCACGACTTAAATGCATATAGGGTTCTCTTTGATGAGAATGGCGATCCTCGGCTTTCATGTTTTGGATTGATGAAAAACAGCAGGGATGGAAAAAGTTACAGCACTAATCTTGCGTACACACCTCCTGAATATCTAAGAAATGGAAGGGTGACTCCAGAAAGTGTTATCTTCAGCTTTGGGACTGTCCTTTTGGATCTTCTAAGTGGAAAACACATCCCTCCTGGTCATGCTCTTGATATGATTCGGGGCAAAAACATTCTTCTCTTAATGGATTCACATCTGGAGGGAAAATTTTCAACTGAAGAAGCAACTGTGGTTTTTGATCTTGCCTCACGATGTTTGCAATATGAACCTAGGGAGCGACCAAATATCAAAGACCTTGTTGCAACAGTTGCTCCATTGCAAAATAAACCTGATGTTCCTTCTTATGTGATGCTGGGAATTCCTAAGCACGAGGAAGCACCTCCGACTCCACAACATCCTCTTTCTGCCATGGGTGATGCCTGTTCAAGGATGGACCTTACGGCCATCCATCAGATTTTGTTAATGACACACTACAAAGATGATGAAGGAAGCAATGAGTTGTCTTTCCAAGAATGGACTCAGCAAATGAAAGATATGTTGGAGGCAAGAAAACGTGGGGACTTGGCATTTCGTGACAAAGATTTTAGATCTGCAATTAACTGCTATTCCCAGTTCATAGATGTTGGGACTATGGTTTCCCCCACGGTGTATGCAAGAAGAAGTCTATGTTATCTCCTCTGTGATCAGCCAGATGCTGCCCTCCGAGATGCAATGCAAGCACAATGTGTCTACCCTGACTGGTCCACTGCATTTTACATGCAGGCGGTTGCTCTTGCCAAGCTAGATATGCACAAGGATGCAGCCGACATGCTGAATGAGGCTGCTGCCCTCGAAGAAAAAAGACATCGGGGAGGCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

511

Amino Acids

57.16

Weight (kDa)

6.29

Isoelectric Point (pI)

42.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	86 - 325	1.6e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	87 - 325	9.8e-25	Protein kinase domain
TPR_BSK1_C	PF25575	363 - 479	4.5e-61	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003677)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35230
fragaria_vesca	FvH4_2g16320 FvH4_2g37600 FvH4_2g37600
malus_domestica	MD05G1131400.v1.1 MD06G1017800.v1.1 MD08G1003600.v1.1 MD10G1134600.v1.1
prunus_persica	Prupe.1G355500_v2.0.a1 Prupe.1G355500_v2.0.a1 Prupe.8G176100_v2.0.a1 Prupe.8G176100_v2.0.a1
pyrus_communis	pycom05g12830 pycom08g00280 pycom10g11610 pycom15g00220
rosa_chinensis	RchiOBHm_Chr6g0280081 RchiOBHm_Chr6g0304131
rosa_laevigata	RLG00000011012 RLG00000013092
rosa_multiflora	Rmu_sc0004617.1_g000013 Rmu_ssc0000218.1_g000005
rosa_roxburghii	Rroxscaffold_7G00164040 Rroxscaffold_7G00188430
rosa_rugosa	Rorug06G0129900 Rorug06G0327900
rosa_samantha	Rh6AG239900 Rh6AG441900 Rh6BG243800 Rh6BG460900 Rh6CG245500 Rh6CG454100 Rh6DG236400
rosa_wichuraiana	Rw6G020920 Rw6G038300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	224
AccB7I	CCANNNNNTGG	1 cut(s)	510
AccBSI	CCGCTC	1 cut(s)	237
AccI	GTMKAC	2 cut(s)	261, 1401
AccII	CGCG	1 cut(s)	105
AciI	CCGC	9 cut(s)	75, 81, 90, 103, 138, 237, 446, 577, 1436
AclWI	GGATC	2 cut(s)	626, 789
AcoI	YGGCCR	2 cut(s)	180, 1113
AcsI	RAATTY	4 cut(s)	170, 314, 878, 1029
AcuI	CTGAAG	4 cut(s)	49, 193, 742, 909
AcyI	GRCGYC	1 cut(s)	271
AdeI	CACNNNGTG	1 cut(s)	1397
AfaI	GTAC	3 cut(s)	452, 587, 704
AfiI	CCNNNNNNNGG	3 cut(s)	122, 510, 1098
AgsI	TTSAA	5 cut(s)	58, 190, 556, 885, 1098
AjnI	CCWGG	2 cut(s)	329, 808
AjuI	GAANNNNNNNTTGG	4 cut(s)	467, 499, 1193, 1225
AluBI	AGCT	7 cut(s)	69, 146, 213, 362, 379, 762, 1453
AluI	AGCT	7 cut(s)	69, 146, 213, 362, 379, 762, 1453
Alw26I	GTCTC	1 cut(s)	232
AlwI	GGATC	2 cut(s)	626, 789
AlwNI	CAGNNNCTG	1 cut(s)	1367
AoxI	GGCC	5 cut(s)	180, 327, 332, 574, 1113
ApeKI	GCWGC	7 cut(s)	9, 417, 436, 1367, 1472, 1493, 1496
ApoI	RAATTY	4 cut(s)	170, 314, 878, 1029
ArsI	GACNNNNNNTTYG	4 cut(s)	277, 309, 760, 792
Asp700I	GAANNNNTTC	2 cut(s)	62, 880
AspA2I	CCTAGG	1 cut(s)	941
AspLEI	GCGC	1 cut(s)	197
AspS9I	GGNCC	2 cut(s)	1105, 1413
AsuC2I	CCSGG	1 cut(s)	159
AsuHPI	GGTGA	2 cut(s)	748, 1097
AvaII	GGWCC	2 cut(s)	1105, 1413
AvrII	CCTAGG	1 cut(s)	941
BauI	CACGAG	1 cut(s)	1040
BbvCI	CCTCAGC	1 cut(s)	380
BbvI	GCAGC	6 cut(s)	404, 423, 1354, 1480, 1483, 1484
BccI	CCATC	4 cut(s)	668, 1096, 1124, 1128
BceAI	ACGGC	3 cut(s)	290, 439, 1128
BcgI	CGANNNNNNTGC	2 cut(s)	581, 615
BciT130I	CCWGG	2 cut(s)	331, 810
BclI	TGATCA	1 cut(s)	1354
BcnI	CCSGG	1 cut(s)	159
BcoDI	GTCTC	1 cut(s)	232
BfaI	CTAG	2 cut(s)	942, 1454
BfmI	CTRYAG	1 cut(s)	562
BfoI	RGCGCY	1 cut(s)	198
BglII	AGATCT	1 cut(s)	1259
BlnI	CCTAGG	1 cut(s)	941
Bme1390I	CCNGG	3 cut(s)	159, 331, 810
Bme18I	GGWCC	2 cut(s)	1105, 1413
BmgT120I	GGNCC	2 cut(s)	1105, 1413
BmiI	GGNNCC	1 cut(s)	130
BmrFI	CCNGG	3 cut(s)	159, 331, 810
BmrI	ACTGGG	2 cut(s)	499, 1276
BmsI	GCATC	6 cut(s)	124, 1011, 1078, 1354, 1369, 1459
BmuI	ACTGGG	2 cut(s)	499, 1276
BpmI	CTGGAG	4 cut(s)	168, 260, 726, 890
Bpu10I	CCTNAGC	2 cut(s)	380, 1035
BpuMI	CCSGG	1 cut(s)	159
BsaBI	GATNNNNATC	1 cut(s)	864
BsaHI	GRCGYC	1 cut(s)	271
BsaJI	CCNNGG	4 cut(s)	635, 941, 1080, 1314
BsaXI	ACNNNNNCTCC	4 cut(s)	197, 227, 967, 997
Bsc4I	CCNNNNNNNGG	3 cut(s)	122, 510, 1098
Bse1I	ACTGG	3 cut(s)	494, 1282, 1415
Bse3DI	GCAATG	4 cut(s)	540, 986, 1171, 1389
Bse8I	GATNNNNATC	1 cut(s)	864
BseBI	CCWGG	2 cut(s)	331, 810
BseDI	CCNNGG	4 cut(s)	635, 941, 1080, 1314
BseGI	GGATG	6 cut(s)	679, 801, 1066, 1107, 1116, 1474
BseJI	GATNNNNATC	1 cut(s)	864
BseLI	CCNNNNNNNGG	3 cut(s)	122, 510, 1098
BseMI	GCAATG	4 cut(s)	540, 986, 1171, 1389
BseMII	CTCAG	2 cut(s)	371, 1205
BseNI	ACTGG	3 cut(s)	494, 1282, 1415
BseRI	GAGGAG	1 cut(s)	1337
BseXI	GCAGC	6 cut(s)	404, 423, 1354, 1480, 1483, 1484
BseYI	CCCAGC	1 cut(s)	1024
Bsh1236I	CGCG	1 cut(s)	105
BshFI	GGCC	5 cut(s)	182, 329, 334, 576, 1115
BsiSI	CCGG	2 cut(s)	141, 158
BslFI	GGGAC	4 cut(s)	282, 781, 1244, 1312
BslI	CCNNNNNNNGG	3 cut(s)	122, 510, 1098
BsmAI	GTCTC	1 cut(s)	232
BsmBI	CGTCTC	1 cut(s)	232
BsmFI	GGGAC	4 cut(s)	282, 781, 1244, 1312
BsnI	GGCC	5 cut(s)	182, 329, 334, 576, 1115
Bsp143I	GATC	5 cut(s)	631, 781, 910, 1259, 1354
Bsp19I	CCATGG	1 cut(s)	1080
BspACI	CCGC	9 cut(s)	75, 81, 90, 103, 138, 237, 446, 577, 1436
BspANI	GGCC	5 cut(s)	182, 329, 334, 576, 1115
BspCNI	CTCAG	2 cut(s)	372, 1204
BspFNI	CGCG	1 cut(s)	105
BspLI	GGNNCC	1 cut(s)	130
BspMAI	CTGCAG	1 cut(s)	566
BspPI	GGATC	2 cut(s)	626, 789
BsrBI	CCGCTC	1 cut(s)	237
BsrDI	GCAATG	4 cut(s)	540, 986, 1171, 1389
BsrI	ACTGG	3 cut(s)	494, 1282, 1415
BssECI	CCNNGG	4 cut(s)	635, 941, 1080, 1314
BssMI	GATC	5 cut(s)	631, 781, 910, 1259, 1354
BssNI	GRCGYC	1 cut(s)	271
BssSI	CACGAG	1 cut(s)	1040
BssT1I	CCWWGG	2 cut(s)	941, 1080
Bst2BI	CACGAG	1 cut(s)	1040
Bst2UI	CCWGG	2 cut(s)	331, 810
Bst4CI	ACNGT	7 cut(s)	117, 125, 293, 772, 901, 979, 1318
BstACI	GRCGYC	1 cut(s)	271
BstAPI	GCANNNNNTGC	1 cut(s)	980
BstC8I	GCNNGC	5 cut(s)	34, 180, 396, 1389, 1434
BstDEI	CTNAG	6 cut(s)	380, 524, 722, 788, 1035, 1191
BstDSI	CCRYGG	2 cut(s)	1080, 1314
BstENI	CCTNNNNNAGG	1 cut(s)	1096
BstF5I	GGATG	6 cut(s)	679, 801, 1066, 1107, 1116, 1474
BstFNI	CGCG	1 cut(s)	105
BstH2I	RGCGCY	1 cut(s)	198
BstHHI	GCGC	1 cut(s)	197
BstKTI	GATC	5 cut(s)	634, 784, 913, 1262, 1357
BstMAI	GTCTC	1 cut(s)	232
BstMBI	GATC	5 cut(s)	631, 781, 910, 1259, 1354
BstMWI	GCNNNNNNNGC	8 cut(s)	89, 143, 359, 391, 423, 980, 1388, 1459
BstNI	CCWGG	2 cut(s)	331, 810
BstNSI	RCATGY	4 cut(s)	32, 457, 1432, 1483
BstSCI	CCNGG	3 cut(s)	157, 329, 808
BstSFI	CTRYAG	1 cut(s)	562
BstUI	CGCG	1 cut(s)	105
BstV1I	GCAGC	6 cut(s)	404, 423, 1354, 1480, 1483, 1484
BstX2I	RGATCY	2 cut(s)	781, 1259
BstYI	RGATCY	2 cut(s)	781, 1259
BsuRI	GGCC	5 cut(s)	182, 329, 334, 576, 1115
BtgI	CCRYGG	2 cut(s)	1080, 1314
BtsCI	GGATG	6 cut(s)	679, 801, 1066, 1107, 1116, 1474
BtsI	GCAGTG	2 cut(s)	159, 1416
BtsIMutI	CAGTG	3 cut(s)	159, 487, 1416
Cac8I	GCNNGC	5 cut(s)	34, 180, 396, 1389, 1434
CaiI	CAGNNNCTG	1 cut(s)	1367
CfoI	GCGC	1 cut(s)	197
Cfr13I	GGNCC	2 cut(s)	1105, 1413
CseI	GACGC	1 cut(s)	279
Csp6I	GTAC	3 cut(s)	451, 586, 703
CviAII	CATG	7 cut(s)	29, 454, 645, 815, 1081, 1429, 1480
CviQI	GTAC	3 cut(s)	451, 586, 703
DdeI	CTNAG	6 cut(s)	380, 524, 722, 788, 1035, 1191
DpnI	GATC	5 cut(s)	633, 783, 912, 1261, 1356
DpnII	GATC	5 cut(s)	631, 781, 910, 1259, 1354
DraIII	CACNNNGTG	1 cut(s)	1397
DrdI	GACNNNNNNGTC	1 cut(s)	224
DseDI	GACNNNNNNGTC	1 cut(s)	224
EaeI	YGGCCR	2 cut(s)	180, 1113
EciI	GGCGGA	1 cut(s)	461
Eco130I	CCWWGG	2 cut(s)	941, 1080
Eco47I	GGWCC	2 cut(s)	1105, 1413
Eco57I	CTGAAG	4 cut(s)	49, 193, 742, 909
EcoNI	CCTNNNNNAGG	1 cut(s)	1096
EcoRI	GAATTC	2 cut(s)	170, 1029
EcoRII	CCWGG	2 cut(s)	329, 808
EcoT14I	CCWWGG	2 cut(s)	941, 1080
EcoT22I	ATGCAT	1 cut(s)	604
ErhI	CCWWGG	2 cut(s)	941, 1080
Esp3I	CGTCTC	1 cut(s)	232
FaeI	CATG	7 cut(s)	32, 457, 648, 818, 1084, 1432, 1483
FaqI	GGGAC	4 cut(s)	282, 781, 1244, 1312
FatI	CATG	7 cut(s)	28, 453, 644, 814, 1080, 1428, 1479
FbaI	TGATCA	1 cut(s)	1354
FblI	GTMKAC	2 cut(s)	261, 1401
FokI	GGATG	6 cut(s)	686, 788, 1053, 1103, 1114, 1481
FspBI	CTAG	2 cut(s)	942, 1454
GlaI	GCGC	1 cut(s)	196
GsaI	CCCAGC	1 cut(s)	1028
GsuI	CTGGAG	4 cut(s)	168, 260, 726, 890
HaeII	RGCGCY	1 cut(s)	198
HaeIII	GGCC	5 cut(s)	182, 329, 334, 576, 1115
HapII	CCGG	2 cut(s)	141, 158
HgaI	GACGC	1 cut(s)	279
HhaI	GCGC	1 cut(s)	197
Hin1I	GRCGYC	1 cut(s)	271
Hin1II	CATG	7 cut(s)	32, 457, 648, 818, 1084, 1432, 1483
Hin6I	GCGC	1 cut(s)	195
HinP1I	GCGC	1 cut(s)	195
HindIII	AAGCTT	1 cut(s)	360
HinfI	GANTC	7 cut(s)	14, 496, 739, 829, 860, 1057, 1189
HpaII	CCGG	2 cut(s)	141, 158
HphI	GGTGA	2 cut(s)	748, 1097
Hpy166II	GTNNAC	4 cut(s)	262, 705, 1402, 1416
Hpy188I	TCNGA	8 cut(s)	169, 217, 232, 501, 537, 1056, 1125, 1376
Hpy188III	TCNNGA	8 cut(s)	277, 310, 713, 743, 821, 869, 921, 1244
Hpy8I	GTNNAC	4 cut(s)	262, 705, 1402, 1416
Hpy99I	CGWCG	3 cut(s)	297, 425, 431
HpyAV	CCTTC	5 cut(s)	73, 566, 726, 1020, 1151
HpyCH4III	ACNGT	7 cut(s)	117, 125, 293, 772, 901, 979, 1318
HpyCH4IV	ACGT	1 cut(s)	1225
HpyF10VI	GCNNNNNNNGC	8 cut(s)	89, 143, 359, 391, 423, 980, 1388, 1459
HpyF3I	CTNAG	6 cut(s)	380, 524, 722, 788, 1035, 1191
HpySE526I	ACGT	1 cut(s)	1225
Hsp92I	GRCGYC	1 cut(s)	271
Hsp92II	CATG	7 cut(s)	32, 457, 648, 818, 1084, 1432, 1483
HspAI	GCGC	1 cut(s)	195
Ksp22I	TGATCA	1 cut(s)	1354
Kzo9I	GATC	5 cut(s)	631, 781, 910, 1259, 1354
LmnI	GCTCC	6 cut(s)	74, 143, 149, 218, 946, 988
Lsp1109I	GCAGC	6 cut(s)	404, 423, 1354, 1480, 1483, 1484
LweI	GCATC	6 cut(s)	124, 1011, 1078, 1354, 1369, 1459
MaeI	CTAG	2 cut(s)	942, 1454
MaeII	ACGT	1 cut(s)	1225
MaeIII	GTNAC	3 cut(s)	683, 736, 1244
MalI	GATC	5 cut(s)	633, 783, 912, 1261, 1356
MbiI	CCGCTC	1 cut(s)	237
MboI	GATC	5 cut(s)	631, 781, 910, 1259, 1354
MboII	GAAGA	6 cut(s)	748, 776, 839, 902, 1341, 1517
MflI	RGATCY	2 cut(s)	781, 1259
MluCI	AATT	9 cut(s)	170, 314, 323, 347, 400, 556, 878, 1029, 1266
MlyI	GAGTC	4 cut(s)	23, 733, 1051, 1183
MmeI	TCCRAC	5 cut(s)	144, 240, 278, 1079, 1191
Mph1103I	ATGCAT	1 cut(s)	604
MroXI	GAANNNNTTC	2 cut(s)	62, 880
MseI	TTAA	4 cut(s)	596, 854, 1133, 1269
MslI	CAYNNNNRTG	1 cut(s)	1467
MspI	CCGG	2 cut(s)	141, 158
MspR9I	CCNGG	3 cut(s)	159, 331, 810
MvaI	CCWGG	2 cut(s)	331, 810
MvnI	CGCG	1 cut(s)	105
MwoI	GCNNNNNNNGC	8 cut(s)	89, 143, 359, 391, 423, 980, 1388, 1459
NciI	CCSGG	1 cut(s)	159
NcoI	CCATGG	1 cut(s)	1080
NdeII	GATC	5 cut(s)	631, 781, 910, 1259, 1354
NlaIII	CATG	7 cut(s)	32, 457, 648, 818, 1084, 1432, 1483
NlaIV	GGNNCC	1 cut(s)	130
NmeAIII	GCCGAG	1 cut(s)	616
NmuCI	GTSAC	2 cut(s)	736, 1244
NsiI	ATGCAT	1 cut(s)	604
NspI	RCATGY	4 cut(s)	32, 457, 1432, 1483
PcsI	WCGNNNNNNNCGW	1 cut(s)	129
PdmI	GAANNNNTTC	2 cut(s)	62, 880
PfeI	GAWTC	3 cut(s)	496, 829, 860
PflMI	CCANNNNNTGG	1 cut(s)	510
PleI	GAGTC	4 cut(s)	22, 733, 1051, 1183
PpsI	GAGTC	4 cut(s)	22, 733, 1051, 1183
Psp6I	CCWGG	2 cut(s)	329, 808
PspFI	CCCAGC	1 cut(s)	1024
PspGI	CCWGG	2 cut(s)	329, 808
PspN4I	GGNNCC	1 cut(s)	130
PspPI	GGNCC	2 cut(s)	1105, 1413
PstI	CTGCAG	1 cut(s)	566
PstNI	CAGNNNCTG	1 cut(s)	1367
PsuI	RGATCY	2 cut(s)	781, 1259
RsaI	GTAC	3 cut(s)	452, 587, 704
RsaNI	GTAC	3 cut(s)	451, 586, 703
RseI	CAYNNNNRTG	1 cut(s)	1467
SaqAI	TTAA	4 cut(s)	596, 854, 1133, 1269
Sau3AI	GATC	5 cut(s)	631, 781, 910, 1259, 1354
Sau96I	GGNCC	2 cut(s)	1105, 1413
SchI	GAGTC	4 cut(s)	23, 733, 1051, 1183
ScrFI	CCNGG	3 cut(s)	159, 331, 810
SfaNI	GCATC	6 cut(s)	124, 1011, 1078, 1354, 1369, 1459
SfcI	CTRYAG	1 cut(s)	562
SinI	GGWCC	2 cut(s)	1105, 1413
SmiMI	CAYNNNNRTG	1 cut(s)	1467
Sse9I	AATT	9 cut(s)	170, 314, 323, 347, 400, 556, 878, 1029, 1266
SsiI	CCGC	9 cut(s)	75, 81, 90, 103, 138, 237, 446, 577, 1436
SspMI	CTAG	2 cut(s)	942, 1454
StyD4I	CCNGG	3 cut(s)	157, 329, 808
StyI	CCWWGG	2 cut(s)	941, 1080
TaaI	ACNGT	7 cut(s)	117, 125, 293, 772, 901, 979, 1318
TaiI	ACGT	1 cut(s)	1228
TaqI	TCGA	1 cut(s)	1503
TasI	AATT	9 cut(s)	170, 314, 323, 347, 400, 556, 878, 1029, 1266
TatI	WGTACW	1 cut(s)	450
TauI	GCSGC	3 cut(s)	140, 240, 579
TfiI	GAWTC	3 cut(s)	496, 829, 860
Tru1I	TTAA	4 cut(s)	596, 854, 1133, 1269
Tru9I	TTAA	4 cut(s)	596, 854, 1133, 1269
TscAI	CASTG	3 cut(s)	159, 494, 1423
TseFI	GTSAC	2 cut(s)	736, 1244
TseI	GCWGC	7 cut(s)	9, 417, 436, 1367, 1472, 1493, 1496
Tsp45I	GTSAC	2 cut(s)	736, 1244
TspDTI	ATGAA	8 cut(s)	211, 372, 633, 674, 951, 1170, 1214, 1276
TspRI	CASTG	3 cut(s)	159, 494, 1423
Van91I	CCANNNNNTGG	1 cut(s)	510
VpaK11BI	GGWCC	2 cut(s)	1105, 1413
XagI	CCTNNNNNAGG	1 cut(s)	1096
XapI	RAATTY	4 cut(s)	170, 314, 878, 1029
XceI	RCATGY	4 cut(s)	32, 457, 1432, 1483
XmaJI	CCTAGG	1 cut(s)	941
XmiI	GTMKAC	2 cut(s)	261, 1401
XmnI	GAANNNNTTC	2 cut(s)	62, 880
XspI	CTAG	2 cut(s)	942, 1454
Zsp2I	ATGCAT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.