Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003677

Physical Location & Seq

Genomic Coordinates Reverse (-)

25689229 .. 25693452

Gene Structure 4224 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1131400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGGTTGCTGTGAATCCAAAGTTTCAGAGAACCAACAACCAGAGAAAATCCAAACCCAGCATCTAGCTCACAATAGCCGGACCCCGACCCACCACACGAATCCCACCTCCGGATCCGACCCGGAAACCGGCGGAGCCCCACCCTTCTCCGAATTCTCCTTCGCCGACCTCAAAGCCGCCACCAACAACTTCAGCTCTGACTACATAGTTTCGGAGAGCGGCGAGAAGGCCCCTAATCTTGTTTACAAGGGCCGGTTGCAGAACCGCCGTTGGATCGCCGTCAAGAAGTTCACCAAGATGGCGTGGCCTGATCCCAAGCAGTTTGCGGAGGAAGCTTGGGGTGTGGGGAAGCTGCGGCATCGGCGGCTTGCGAATTTGATTGGGTATTGCTGTGATGGCGATGAGAGGCTGCTTGTTGCTGAGTACATGCCTACTGATACTTTGGCTAAGCATTTGTTCCACTGGGAGAATCAAACCATTGAGTGGGCCATGCGTTTAAGAGTGGCTCTTTATATTGCTGAAGCTTTAGATTATTGCAGTACCGAGGGCCGCCCGTTATACCACGATTTGAATGCTTATAGGGTTCTCTTTGACGAGGATGGTGATCCTCGTCTTTCATGTTTTGGCTTGATGAGAAATAGTAGGGATGGGAAGAGTTACAGCACAAATCTTGCTTACACGCCACCTGAATATTTAAGAAATGGAAGGGTCACTCCAGAAAGTGTTGTTTATAGCTTTGGCACCGTCCTTCTAGATCTGCTTAGTGGGAAGCACATCCCTCCAAGTCATGCACTTGACATGATACGAGGGAAAAATATCATTCTGTTAATGGATTCACATTTGGAGGGGAAATTTTCTACAGAGGAAGCAACGGTAGTTGTTAATCTTGCCTCTCAATGTCTCCAATATGAACCTAGAGAGCGGCCTAATACAAAGGACCTTGTTGCAACACTCGCTCCACTGCAGACCAAACCTGATGTTCCATCTTATGTCATGCTTGGAATTCCAAAGCATGAAGAAGCGCCTTCAACCCCACAACGTCCCCTTTCTGCTATGGGTGACGCCTGCTCTCGGATGGACCTCACAGCAATCCATCAGATCTTGGTTATGACGCACTACAGAGATGATGAAGGAACCAATGAGTTATCTTTCCAAGAGTGGACTCAACAGATGAGAGACATGTTGGAGGCAAGGAAACGAGGGGATTATGCATTTCGGGACAAAGATTTTAAAGCAGCCATTGAGTGTTACTCCCAGTTCATAGATGTTGGAACTATGGTTTCGCCAACTGTTTTTGCCCGACGCAGTTTATGCTACCTGTTAAATGAGCAACCAGATGCTGCCCTTCGAGATGCAATGCAAGCGCAATGTGTCTATCCTGAGTGGCCCACAGCCTTCTACATGCAGTCAGTTGCTCTAGCCAAGCTGGACATGCACAAGGATGCCGCTGACATGTTGAACGAAGCGACTGCGCTAGAAGAAAAGAAGCAGCGAGGAGGCCGGGGATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

504

Amino Acids

56.9

Weight (kDa)

5.88

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	81 - 318	5.1e-26	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	81 - 316	3.8e-23	Protein kinase domain
TPR_BSK1_C	PF25575	354 - 470	4e-61	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003677)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35230
fragaria_vesca	FvH4_2g16320 FvH4_2g37600 FvH4_2g37600
malus_domestica	MD05G1131400.v1.1 MD06G1017800.v1.1 MD08G1003600.v1.1 MD10G1134600.v1.1
prunus_persica	Prupe.1G355500_v2.0.a1 Prupe.1G355500_v2.0.a1 Prupe.8G176100_v2.0.a1 Prupe.8G176100_v2.0.a1
pyrus_communis	pycom05g12830 pycom08g00280 pycom10g11610 pycom15g00220
rosa_chinensis	RchiOBHm_Chr6g0280081 RchiOBHm_Chr6g0304131
rosa_laevigata	RLG00000011012 RLG00000013092
rosa_multiflora	Rmu_sc0004617.1_g000013 Rmu_ssc0000218.1_g000005
rosa_roxburghii	Rroxscaffold_7G00164040 Rroxscaffold_7G00188430
rosa_rugosa	Rorug06G0129900 Rorug06G0327900
rosa_samantha	Rh6AG239900 Rh6AG441900 Rh6BG243800 Rh6BG460900 Rh6CG245500 Rh6CG454100 Rh6DG236400
rosa_wichuraiana	Rw6G020920 Rw6G038300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	740
AccB7I	CCANNNNNTGG	1 cut(s)	483
AccBSI	CCGCTC	2 cut(s)	219, 922
AccIII	TCCGGA	1 cut(s)	110
AclWI	GGATC	5 cut(s)	108, 121, 281, 305, 599
AcsI	RAATTY	4 cut(s)	152, 373, 851, 1002
AcuI	CTGAAG	2 cut(s)	175, 540
AcyI	GRCGYC	1 cut(s)	1062
AfaI	GTAC	2 cut(s)	425, 541
AfiI	CCNNNNNNNGG	4 cut(s)	110, 128, 483, 1071
AflIII	ACRYGT	2 cut(s)	1179, 1452
AgsI	TTSAA	3 cut(s)	571, 1029, 1459
AluBI	AGCT	7 cut(s)	68, 195, 335, 352, 524, 735, 1426
AluI	AGCT	7 cut(s)	68, 195, 335, 352, 524, 735, 1426
Alw26I	GTCTC	2 cut(s)	905, 1170
AlwI	GGATC	5 cut(s)	108, 121, 281, 305, 599
AlwNI	CAGNNNCTG	1 cut(s)	1340
Aor13HI	TCCGGA	1 cut(s)	110
AoxI	GGCC	8 cut(s)	228, 250, 305, 486, 547, 923, 1385, 1498
ApeKI	GCWGC	5 cut(s)	352, 409, 1235, 1340, 1489
ApoI	RAATTY	4 cut(s)	152, 373, 851, 1002
AspLEI	GCGC	3 cut(s)	1024, 1366, 1474
AspS9I	GGNCC	8 cut(s)	81, 229, 250, 486, 547, 937, 1078, 1386
AsuC2I	CCSGG	2 cut(s)	122, 1502
AsuHPI	GGTGA	3 cut(s)	283, 614, 1070
AvaII	GGWCC	3 cut(s)	81, 937, 1078
BamHI	GGATCC	1 cut(s)	113
BanI	GGYRCC	1 cut(s)	740
BanII	GRGCYC	1 cut(s)	139
BbvI	GCAGC	5 cut(s)	339, 396, 1247, 1327, 1501
BccI	CCATC	7 cut(s)	292, 389, 593, 641, 991, 1069, 1101
BceAI	ACGGC	2 cut(s)	252, 263
BcgI	CGANNNNNNTGC	4 cut(s)	554, 588, 1451, 1485
BcnI	CCSGG	2 cut(s)	122, 1502
BcoDI	GTCTC	2 cut(s)	905, 1170
BfaI	CTAG	5 cut(s)	65, 752, 915, 1418, 1475
BfmI	CTRYAG	3 cut(s)	858, 962, 1117
BfoI	RGCGCY	1 cut(s)	1025
BglII	AGATCT	2 cut(s)	754, 1098
BlpI	GCTNAGC	1 cut(s)	447
Bme1390I	CCNGG	2 cut(s)	122, 1502
Bme18I	GGWCC	3 cut(s)	81, 937, 1078
BmgT120I	GGNCC	8 cut(s)	81, 229, 250, 486, 547, 937, 1078, 1386
BmiI	GGNNCC	6 cut(s)	83, 115, 136, 231, 742, 1135
BmrFI	CCNGG	2 cut(s)	122, 1502
BmrI	ACTGGG	2 cut(s)	472, 1249
BmsI	GCATC	5 cut(s)	70, 367, 1327, 1342, 1432
BmuI	ACTGGG	2 cut(s)	472, 1249
BplI	GAGNNNNNCTC	2 cut(s)	1235, 1267
BpmI	CTGGAG	1 cut(s)	699
Bpu1102I	GCTNAGC	1 cut(s)	447
BpuMI	CCSGG	2 cut(s)	122, 1502
BsaBI	GATNNNNATC	1 cut(s)	603
BsaHI	GRCGYC	1 cut(s)	1062
BsaJI	CCNNGG	2 cut(s)	543, 1501
BsaWI	WCCGGW	1 cut(s)	110
BsaXI	ACNNNNNCTCC	2 cut(s)	940, 970
Bsc4I	CCNNNNNNNGG	4 cut(s)	110, 128, 483, 1071
Bse118I	RCCGGY	2 cut(s)	128, 252
Bse1I	ACTGG	2 cut(s)	467, 1255
Bse3DI	GCAATG	2 cut(s)	1362, 1373
Bse8I	GATNNNNATC	1 cut(s)	603
BseAI	TCCGGA	1 cut(s)	110
BseDI	CCNNGG	2 cut(s)	543, 1501
BseGI	GGATG	5 cut(s)	604, 652, 774, 1080, 1447
BseJI	GATNNNNATC	1 cut(s)	603
BseLI	CCNNNNNNNGG	4 cut(s)	110, 128, 483, 1071
BseMI	GCAATG	2 cut(s)	1362, 1373
BseMII	CTCAG	2 cut(s)	411, 1371
BseNI	ACTGG	2 cut(s)	467, 1255
BseRI	GAGGAG	1 cut(s)	1509
BseXI	GCAGC	5 cut(s)	339, 396, 1247, 1327, 1501
BseYI	CCCAGC	1 cut(s)	57
BshFI	GGCC	8 cut(s)	230, 252, 307, 488, 549, 925, 1387, 1500
BshNI	GGYRCC	1 cut(s)	740
BsiSI	CCGG	6 cut(s)	79, 111, 122, 129, 253, 1501
BslFI	GGGAC	2 cut(s)	1026, 1232
BslI	CCNNNNNNNGG	4 cut(s)	110, 128, 483, 1071
BsmAI	GTCTC	2 cut(s)	905, 1170
BsmFI	GGGAC	2 cut(s)	1026, 1232
BsmI	GAATGC	1 cut(s)	577
BsnI	GGCC	8 cut(s)	230, 252, 307, 488, 549, 925, 1387, 1500
Bsp1286I	GDGCHC	1 cut(s)	139
Bsp13I	TCCGGA	1 cut(s)	110
Bsp143I	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
Bsp1720I	GCTNAGC	1 cut(s)	447
BspANI	GGCC	8 cut(s)	230, 252, 307, 488, 549, 925, 1387, 1500
BspCNI	CTCAG	2 cut(s)	412, 1372
BspEI	TCCGGA	1 cut(s)	110
BspHI	TCATGA	1 cut(s)	1508
BspLI	GGNNCC	6 cut(s)	83, 115, 136, 231, 742, 1135
BspMAI	CTGCAG	1 cut(s)	966
BspPI	GGATC	5 cut(s)	108, 121, 281, 305, 599
BspT107I	GGYRCC	1 cut(s)	740
BsrBI	CCGCTC	2 cut(s)	219, 922
BsrDI	GCAATG	2 cut(s)	1362, 1373
BsrFI	RCCGGY	2 cut(s)	128, 252
BsrI	ACTGG	2 cut(s)	467, 1255
BssAI	RCCGGY	2 cut(s)	128, 252
BssECI	CCNNGG	2 cut(s)	543, 1501
BssMI	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
BssNI	GRCGYC	1 cut(s)	1062
Bst4CI	ACNGT	3 cut(s)	745, 874, 1291
Bst6I	CTCTTC	1 cut(s)	647
BstACI	GRCGYC	1 cut(s)	1062
BstAPI	GCANNNNNTGC	1 cut(s)	1311
BstC8I	GCNNGC	3 cut(s)	369, 1066, 1362
BstDEI	CTNAG	4 cut(s)	420, 447, 761, 1380
BstF5I	GGATG	5 cut(s)	604, 652, 774, 1080, 1447
BstH2I	RGCGCY	1 cut(s)	1025
BstHHI	GCGC	3 cut(s)	1024, 1366, 1474
BstKTI	GATC	7 cut(s)	116, 276, 313, 607, 757, 1101, 1508
BstMAI	GTCTC	2 cut(s)	905, 1170
BstMBI	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
BstMWI	GCNNNNNNNGC	8 cut(s)	332, 361, 364, 396, 953, 1311, 1361, 1432
BstNSI	RCATGY	5 cut(s)	430, 1183, 1405, 1435, 1456
BstSCI	CCNGG	2 cut(s)	120, 1500
BstSFI	CTRYAG	3 cut(s)	858, 962, 1117
BstV1I	GCAGC	5 cut(s)	339, 396, 1247, 1327, 1501
BstX2I	RGATCY	3 cut(s)	113, 754, 1098
BstYI	RGATCY	3 cut(s)	113, 754, 1098
BsuRI	GGCC	8 cut(s)	230, 252, 307, 488, 549, 925, 1387, 1500
BtgZI	GCGATG	1 cut(s)	414
BtsCI	GGATG	5 cut(s)	604, 652, 774, 1080, 1447
BtsI	GCAGTG	1 cut(s)	959
BtsIMutI	CAGTG	2 cut(s)	460, 959
Cac8I	GCNNGC	3 cut(s)	369, 1066, 1362
CaiI	CAGNNNCTG	1 cut(s)	1340
CciI	TCATGA	1 cut(s)	1508
CfoI	GCGC	3 cut(s)	1024, 1366, 1474
Cfr10I	RCCGGY	2 cut(s)	128, 252
Cfr13I	GGNCC	8 cut(s)	81, 229, 250, 486, 547, 937, 1078, 1386
CseI	GACGC	3 cut(s)	1070, 1120, 1311
Csp6I	GTAC	2 cut(s)	424, 540
CviQI	GTAC	2 cut(s)	424, 540
DdeI	CTNAG	4 cut(s)	420, 447, 761, 1380
DpnI	GATC	7 cut(s)	115, 275, 312, 606, 756, 1100, 1507
DpnII	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
DraI	TTTAAA	1 cut(s)	1231
Eam1104I	CTCTTC	1 cut(s)	647
EarI	CTCTTC	1 cut(s)	647
EciI	GGCGGA	1 cut(s)	147
Eco24I	GRGCYC	1 cut(s)	139
Eco47I	GGWCC	3 cut(s)	81, 937, 1078
Eco57I	CTGAAG	2 cut(s)	175, 540
EcoO109I	RGGNCCY	2 cut(s)	229, 937
EcoRI	GAATTC	2 cut(s)	152, 1002
EcoT22I	ATGCAT	1 cut(s)	1213
EcoT38I	GRGCYC	1 cut(s)	139
FaqI	GGGAC	2 cut(s)	1026, 1232
FokI	GGATG	5 cut(s)	611, 659, 761, 1087, 1454
FriOI	GRGCYC	1 cut(s)	139
FspBI	CTAG	5 cut(s)	65, 752, 915, 1418, 1475
GlaI	GCGC	3 cut(s)	1023, 1365, 1473
GsaI	CCCAGC	1 cut(s)	61
GsuI	CTGGAG	1 cut(s)	699
HaeII	RGCGCY	1 cut(s)	1025
HaeIII	GGCC	8 cut(s)	230, 252, 307, 488, 549, 925, 1387, 1500
HapII	CCGG	6 cut(s)	79, 111, 122, 129, 253, 1501
HgaI	GACGC	3 cut(s)	1070, 1120, 1311
HhaI	GCGC	3 cut(s)	1024, 1366, 1474
Hin1I	GRCGYC	1 cut(s)	1062
Hin6I	GCGC	3 cut(s)	1022, 1364, 1472
HinP1I	GCGC	3 cut(s)	1022, 1364, 1472
HindIII	AAGCTT	2 cut(s)	333, 522
HinfI	GANTC	5 cut(s)	14, 100, 469, 833, 1162
HpaII	CCGG	6 cut(s)	79, 111, 122, 129, 253, 1501
HphI	GGTGA	3 cut(s)	283, 614, 1070
Hpy166II	GTNNAC	3 cut(s)	244, 291, 1161
Hpy188I	TCNGA	7 cut(s)	28, 118, 151, 199, 214, 1074, 1098
Hpy188III	TCNNGA	8 cut(s)	111, 283, 716, 752, 1217, 1349, 1379, 1509
Hpy8I	GTNNAC	3 cut(s)	244, 291, 1161
Hpy99I	CGWCG	1 cut(s)	1305
HpyAV	CCTTC	9 cut(s)	154, 169, 220, 699, 758, 1035, 1124, 1355, 1405
HpyCH4III	ACNGT	3 cut(s)	745, 874, 1291
HpyCH4IV	ACGT	1 cut(s)	1039
HpyF10VI	GCNNNNNNNGC	8 cut(s)	332, 361, 364, 396, 953, 1311, 1361, 1432
HpyF3I	CTNAG	4 cut(s)	420, 447, 761, 1380
HpySE526I	ACGT	1 cut(s)	1039
Hsp92I	GRCGYC	1 cut(s)	1062
HspAI	GCGC	3 cut(s)	1022, 1364, 1472
Kpn2I	TCCGGA	1 cut(s)	110
Kzo9I	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
LmnI	GCTCC	2 cut(s)	134, 961
Lsp1109I	GCAGC	5 cut(s)	339, 396, 1247, 1327, 1501
LweI	GCATC	5 cut(s)	70, 367, 1327, 1342, 1432
MaeI	CTAG	5 cut(s)	65, 752, 915, 1418, 1475
MaeII	ACGT	1 cut(s)	1039
MaeIII	GTNAC	4 cut(s)	656, 709, 1058, 1247
MalI	GATC	7 cut(s)	115, 275, 312, 606, 756, 1100, 1507
MbiI	CCGCTC	2 cut(s)	219, 922
MboI	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
MboII	GAAGA	3 cut(s)	664, 1028, 1490
MflI	RGATCY	3 cut(s)	113, 754, 1098
MhlI	GDGCHC	1 cut(s)	139
MluCI	AATT	4 cut(s)	152, 373, 851, 1002
MlyI	GAGTC	1 cut(s)	1156
MmeI	TCCRAC	4 cut(s)	141, 251, 1164, 1249
Mph1103I	ATGCAT	1 cut(s)	1213
MroI	TCCGGA	1 cut(s)	110
MseI	TTAA	6 cut(s)	497, 695, 827, 882, 1230, 1322
MslI	CAYNNNNRTG	2 cut(s)	296, 1440
MspA1I	CMGCKG	1 cut(s)	1448
MspI	CCGG	6 cut(s)	79, 111, 122, 129, 253, 1501
MspR9I	CCNGG	2 cut(s)	122, 1502
Mva1269I	GAATGC	1 cut(s)	577
MwoI	GCNNNNNNNGC	8 cut(s)	332, 361, 364, 396, 953, 1311, 1361, 1432
NciI	CCSGG	2 cut(s)	122, 1502
NdeII	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
NlaIV	GGNNCC	6 cut(s)	83, 115, 136, 231, 742, 1135
NmuCI	GTSAC	2 cut(s)	709, 1058
NsiI	ATGCAT	1 cut(s)	1213
NspI	RCATGY	5 cut(s)	430, 1183, 1405, 1435, 1456
PagI	TCATGA	1 cut(s)	1508
PciI	ACATGT	2 cut(s)	1179, 1452
PctI	GAATGC	1 cut(s)	577
PfeI	GAWTC	4 cut(s)	14, 100, 469, 833
PflMI	CCANNNNNTGG	1 cut(s)	483
PleI	GAGTC	1 cut(s)	1156
PpsI	GAGTC	1 cut(s)	1156
PpuMI	RGGWCCY	1 cut(s)	937
PscI	ACATGT	2 cut(s)	1179, 1452
Psp5II	RGGWCCY	1 cut(s)	937
PspFI	CCCAGC	1 cut(s)	57
PspN4I	GGNNCC	6 cut(s)	83, 115, 136, 231, 742, 1135
PspPI	GGNCC	8 cut(s)	81, 229, 250, 486, 547, 937, 1078, 1386
PspPPI	RGGWCCY	1 cut(s)	937
PstI	CTGCAG	1 cut(s)	966
PstNI	CAGNNNCTG	1 cut(s)	1340
PsuI	RGATCY	3 cut(s)	113, 754, 1098
RsaI	GTAC	2 cut(s)	425, 541
RsaNI	GTAC	2 cut(s)	424, 540
RseI	CAYNNNNRTG	2 cut(s)	296, 1440
SaqAI	TTAA	6 cut(s)	497, 695, 827, 882, 1230, 1322
Sau3AI	GATC	7 cut(s)	113, 273, 310, 604, 754, 1098, 1505
Sau96I	GGNCC	8 cut(s)	81, 229, 250, 486, 547, 937, 1078, 1386
SchI	GAGTC	1 cut(s)	1156
ScrFI	CCNGG	2 cut(s)	122, 1502
SduI	GDGCHC	1 cut(s)	139
SfaNI	GCATC	5 cut(s)	70, 367, 1327, 1342, 1432
SfcI	CTRYAG	3 cut(s)	858, 962, 1117
SinI	GGWCC	3 cut(s)	81, 937, 1078
SmiMI	CAYNNNNRTG	2 cut(s)	296, 1440
Sse9I	AATT	4 cut(s)	152, 373, 851, 1002
SspI	AATATT	1 cut(s)	692
SspMI	CTAG	5 cut(s)	65, 752, 915, 1418, 1475
StyD4I	CCNGG	2 cut(s)	120, 1500
TaaI	ACNGT	3 cut(s)	745, 874, 1291
TaiI	ACGT	1 cut(s)	1042
TaqI	TCGA	1 cut(s)	1348
TasI	AATT	4 cut(s)	152, 373, 851, 1002
TatI	WGTACW	1 cut(s)	423
TauI	GCSGC	7 cut(s)	179, 222, 358, 367, 552, 925, 1448
TfiI	GAWTC	4 cut(s)	14, 100, 469, 833
Tru1I	TTAA	6 cut(s)	497, 695, 827, 882, 1230, 1322
Tru9I	TTAA	6 cut(s)	497, 695, 827, 882, 1230, 1322
TscAI	CASTG	2 cut(s)	467, 966
TseFI	GTSAC	2 cut(s)	709, 1058
TseI	GCWGC	5 cut(s)	352, 409, 1235, 1340, 1489
Tsp45I	GTSAC	2 cut(s)	709, 1058
TspDTI	ATGAA	5 cut(s)	606, 924, 1029, 1143, 1249
TspRI	CASTG	2 cut(s)	467, 966
Van91I	CCANNNNNTGG	1 cut(s)	483
VpaK11BI	GGWCC	3 cut(s)	81, 937, 1078
XapI	RAATTY	4 cut(s)	152, 373, 851, 1002
XbaI	TCTAGA	1 cut(s)	751
XceI	RCATGY	5 cut(s)	430, 1183, 1405, 1435, 1456
XspI	CTAG	5 cut(s)	65, 752, 915, 1418, 1475
Zsp2I	ATGCAT	1 cut(s)	1213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.