Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006799

Physical Location & Seq

Genomic Coordinates Forward (+)

16810482 .. 16812077

Gene Structure 1596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G35350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGGCTTTTTCTGCACCATCACTTTCCAAATTCTCTCTTTTGGTTGCCATTTCAGCATCAGCTCTCCTCTGTTGTGCTTTTGCCCGTGATTTCTCCATTGTTGGATACACGCCGGAGCATTTGACAAACACTGACAAGCTTCTAGAGCTCTTCGAGTCATGGATGTCAGAACACAGCAAGGCTTACAAAAGCGTGGAGGAGAAGGTGCACAGGTTTGAGGTTTTCAGAGAGAATCTGATGCATATAGACCAGAGGAACAATGAGATCAACAGTTACTGGCTCGGTTTGAACGAGTTTGCGGATTTGACCCATGAAGAGTTCAAAGGAAGATATCTAGGACTTGCAAAGCCACAATTCTCTAGAAAGAGACAGCCCTCAGCTAACTTCAGGTACAGAGATATCACGGACTTGCCTAAATCCGTAGACTGGAGAAAGAAAGGCGCTGTGGCTCCTGTCAAGGACCAGGGTCAATGTGGTAGCTGTTGGGCATTTTCAACAGTTGCAGCTGTCGAGGGGATCAACCAGATCACAACAGGGAATCTGAGTTCGCTTTCAGAGCAAGAACTCATAGACTGTGACACAACTTTCAACAGTGGCTGCAATGGAGGTCTCATGGACTACGCATTCCAGTACATAATTTCGACCGGTGGTCTCCACAAAGAAGATGATTACCCTTATCTCATGGAGGAAGGAATTTGTCAAGAGCAGAAAGAGGATGTGGAACGTGTGACAATCAGCGGCTACGAAGATGTCCCTGAAAATGATGACGAAAGCCTGGTGAAGGCTTTAGCTCATCAGCCAGTCAGTGTGGCTATTGAGGCTTCAGGAAGAGACTTCCAGTTCTACAAAGGGGGAGTGTTTAATGGTAAATGTGGAACAGACCTAGACCACGGTGTGGCAGCGGTTGGATATGGTTCATCAAAGGGATCTGACTATGTTATTGTCAAGAACTCATGGGGACCAAGATGGGGAGAGAAAGGGTTTATTAGGATGAAGAGAAACACTGGTAAACCAGAGGGACTCTGTGGAATCAACAAGATGGCCTCATATCCTACCAAGACCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000325 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010623 GO:0012501 GO:0016787 GO:0019538 GO:0030154 GO:0030163 GO:0030312 GO:0032502 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0070011 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

355

Amino Acids

39.62

Weight (kDa)

5.6

Isoelectric Point (pI)

31.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	51 - 107	3.6e-19	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	137 - 352	2.6e-83	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006799)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20850 AT4G35350 AT4G35350
fragaria_vesca	FvH4_2g16940
malus_domestica	MD05G1141100.v1.1 MD10G1142500.v1.1 MD11G1242400.v1.1
prunus_persica	Prupe.8G181100_v2.0.a1
pyrus_communis	pycom05g13650
rosa_chinensis	RchiOBHm_Chr6g0281081 RchiOBHm_Chr6g0281131
rosa_laevigata	RLG00000012992 RLG00000012998
rosa_multiflora	Rmu_co8502169.1_g000002 Rmu_sc0001705.1_g000005 Rmu_sc0001705.1_g000018
rosa_roxburghii	Rroxscaffold_7G00187270 Rroxscaffold_7G00187300 Rroxscaffold_7G00187320
rosa_rugosa	Rorug06G0138900 Rorug06G0139000 Rorug06G0139100
rosa_samantha	Rh6BG253900 Rh6BG254100
rosa_wichuraiana	Rw6G021770 Rw6G021780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	895
AccI	GTMKAC	1 cut(s)	423
AciI	CCGC	3 cut(s)	299, 738, 902
AclWI	GGATC	2 cut(s)	524, 934
AcsI	RAATTY	2 cut(s)	29, 693
AcuI	CTGAAG	2 cut(s)	370, 807
AdeI	CACNNNGTG	1 cut(s)	895
AfaI	GTAC	2 cut(s)	392, 632
AfiI	CCNNNNNNNGG	4 cut(s)	426, 781, 895, 968
AflIII	ACRYGT	1 cut(s)	724
AgeI	ACCGGT	1 cut(s)	644
AgsI	TTSAA	4 cut(s)	289, 322, 495, 589
AhdI	GACNNNNNGTC	1 cut(s)	648
AjnI	CCWGG	2 cut(s)	462, 774
AloI	GAACNNNNNNTCC	2 cut(s)	529, 561
AluBI	AGCT	7 cut(s)	62, 139, 148, 380, 480, 506, 791
AluI	AGCT	7 cut(s)	62, 139, 148, 380, 480, 506, 791
Alw21I	GWGCWC	2 cut(s)	150, 210
Alw26I	GTCTC	4 cut(s)	361, 614, 656, 825
Alw44I	GTGCAC	1 cut(s)	206
AlwI	GGATC	2 cut(s)	524, 934
AlwNI	CAGNNNCTG	2 cut(s)	276, 597
AoxI	GGCC	1 cut(s)	1041
ApaLI	GTGCAC	1 cut(s)	206
ApeKI	GCWGC	3 cut(s)	503, 597, 899
ApoI	RAATTY	2 cut(s)	29, 693
AsiGI	ACCGGT	1 cut(s)	644
AspLEI	GCGC	1 cut(s)	443
AspS9I	GGNCC	2 cut(s)	460, 959
AsuHPI	GGTGA	1 cut(s)	790
AvaII	GGWCC	2 cut(s)	460, 959
BaeGI	GKGCMC	1 cut(s)	210
BanII	GRGCYC	1 cut(s)	150
Bbv12I	GWGCWC	2 cut(s)	150, 210
BbvCI	CCTCAGC	1 cut(s)	376
BbvI	GCAGC	3 cut(s)	515, 584, 911
BccI	CCATC	3 cut(s)	25, 960, 1033
BciT130I	CCWGG	2 cut(s)	464, 776
BciVI	GTATCC	1 cut(s)	98
BcoDI	GTCTC	4 cut(s)	361, 614, 656, 825
BfaI	CTAG	4 cut(s)	143, 335, 360, 884
BfoI	RGCGCY	1 cut(s)	444
BfuI	GTATCC	1 cut(s)	98
BisI	GCNGC	4 cut(s)	504, 598, 739, 900
BlsI	GCNGC	4 cut(s)	505, 599, 740, 901
Bme1390I	CCNGG	2 cut(s)	464, 776
Bme18I	GGWCC	2 cut(s)	460, 959
BmeRI	GACNNNNNGTC	1 cut(s)	648
BmgT120I	GGNCC	2 cut(s)	460, 959
BmiI	GGNNCC	2 cut(s)	450, 960
BmrFI	CCNGG	2 cut(s)	464, 776
BmsI	GCATC	2 cut(s)	65, 228
BoxI	GACNNNNGTC	1 cut(s)	465
BpmI	CTGGAG	1 cut(s)	448
Bpu10I	CCTNAGC	1 cut(s)	376
BsaI	GGTCTC	2 cut(s)	614, 656
BsaJI	CCNNGG	2 cut(s)	463, 889
BsaWI	WCCGGW	1 cut(s)	644
Bsc4I	CCNNNNNNNGG	4 cut(s)	426, 781, 895, 968
Bse118I	RCCGGY	1 cut(s)	644
Bse1I	ACTGG	6 cut(s)	281, 431, 628, 800, 838, 1009
Bse3DI	GCAATG	1 cut(s)	607
BseBI	CCWGG	2 cut(s)	464, 776
BseDI	CCNNGG	2 cut(s)	463, 889
BseGI	GGATG	3 cut(s)	168, 721, 996
BseLI	CCNNNNNNNGG	4 cut(s)	426, 781, 895, 968
BseMI	GCAATG	1 cut(s)	607
BseMII	CTCAG	2 cut(s)	390, 533
BseNI	ACTGG	6 cut(s)	281, 431, 628, 800, 838, 1009
BseRI	GAGGAG	2 cut(s)	56, 212
BseSI	GKGCMC	1 cut(s)	210
BseXI	GCAGC	3 cut(s)	515, 584, 911
Bsh1285I	CGRYCG	1 cut(s)	645
BshFI	GGCC	1 cut(s)	1043
BshTI	ACCGGT	1 cut(s)	644
BsiEI	CGRYCG	1 cut(s)	645
BsiHKAI	GWGCWC	2 cut(s)	150, 210
BsiSI	CCGG	2 cut(s)	113, 645
BslFI	GGGAC	3 cut(s)	737, 972, 1032
BslI	CCNNNNNNNGG	4 cut(s)	426, 781, 895, 968
BsmAI	GTCTC	4 cut(s)	361, 614, 656, 825
BsmFI	GGGAC	3 cut(s)	737, 972, 1032
BsmI	GAATGC	1 cut(s)	623
BsnI	GGCC	1 cut(s)	1043
Bso31I	GGTCTC	2 cut(s)	614, 656
Bsp1286I	GDGCHC	2 cut(s)	150, 210
Bsp143I	GATC	4 cut(s)	264, 516, 525, 926
BspACI	CCGC	3 cut(s)	299, 738, 902
BspANI	GGCC	1 cut(s)	1043
BspCNI	CTCAG	2 cut(s)	389, 534
BspLI	GGNNCC	2 cut(s)	450, 960
BspPI	GGATC	2 cut(s)	524, 934
BspQI	GCTCTTC	1 cut(s)	155
BspTNI	GGTCTC	2 cut(s)	614, 656
BsrDI	GCAATG	1 cut(s)	607
BsrFI	RCCGGY	1 cut(s)	644
BsrI	ACTGG	6 cut(s)	281, 431, 628, 800, 838, 1009
BssAI	RCCGGY	1 cut(s)	644
BssECI	CCNNGG	2 cut(s)	463, 889
BssMI	GATC	4 cut(s)	264, 516, 525, 926
Bst2UI	CCWGG	2 cut(s)	464, 776
Bst4CI	ACNGT	5 cut(s)	272, 499, 575, 593, 893
Bst6I	CTCTTC	4 cut(s)	155, 309, 823, 989
BstDEI	CTNAG	2 cut(s)	376, 542
BstDSI	CCRYGG	1 cut(s)	889
BstENI	CCTNNNNNAGG	1 cut(s)	779
BstF5I	GGATG	3 cut(s)	168, 721, 996
BstH2I	RGCGCY	1 cut(s)	444
BstHHI	GCGC	1 cut(s)	443
BstKTI	GATC	4 cut(s)	267, 519, 528, 929
BstMAI	GTCTC	4 cut(s)	361, 614, 656, 825
BstMBI	GATC	4 cut(s)	264, 516, 525, 926
BstMCI	CGRYCG	1 cut(s)	645
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 53, 145, 556, 818
BstNI	CCWGG	2 cut(s)	464, 776
BstPAI	GACNNNNGTC	1 cut(s)	465
BstSCI	CCNGG	2 cut(s)	462, 774
BstSLI	GKGCMC	1 cut(s)	210
BstV1I	GCAGC	3 cut(s)	515, 584, 911
BstX2I	RGATCY	1 cut(s)	926
BstYI	RGATCY	1 cut(s)	926
BsuI	GTATCC	1 cut(s)	98
BsuRI	GGCC	1 cut(s)	1043
BtgI	CCRYGG	1 cut(s)	889
BtsCI	GGATG	3 cut(s)	168, 721, 996
BtsIMutI	CAGTG	4 cut(s)	129, 598, 811, 1002
CaiI	CAGNNNCTG	2 cut(s)	276, 597
CfoI	GCGC	1 cut(s)	443
Cfr10I	RCCGGY	1 cut(s)	644
Cfr13I	GGNCC	2 cut(s)	460, 959
Csp6I	GTAC	2 cut(s)	391, 631
CspAI	ACCGGT	1 cut(s)	644
CviAII	CATG	5 cut(s)	159, 311, 613, 682, 954
CviQI	GTAC	2 cut(s)	391, 631
DdeI	CTNAG	2 cut(s)	376, 542
DpnI	GATC	4 cut(s)	266, 518, 527, 928
DpnII	GATC	4 cut(s)	264, 516, 525, 926
DraIII	CACNNNGTG	1 cut(s)	895
DriI	GACNNNNNGTC	1 cut(s)	648
Eam1104I	CTCTTC	4 cut(s)	155, 309, 823, 989
Eam1105I	GACNNNNNGTC	1 cut(s)	648
EarI	CTCTTC	4 cut(s)	155, 309, 823, 989
Ecl136II	GAGCTC	1 cut(s)	148
Eco24I	GRGCYC	1 cut(s)	150
Eco31I	GGTCTC	2 cut(s)	614, 656
Eco32I	GATATC	2 cut(s)	332, 400
Eco47I	GGWCC	2 cut(s)	460, 959
Eco53kI	GAGCTC	1 cut(s)	148
Eco57I	CTGAAG	2 cut(s)	370, 807
EcoICRI	GAGCTC	1 cut(s)	148
EcoNI	CCTNNNNNAGG	1 cut(s)	779
EcoRII	CCWGG	2 cut(s)	462, 774
EcoRV	GATATC	2 cut(s)	332, 400
EcoT22I	ATGCAT	1 cut(s)	243
EcoT38I	GRGCYC	1 cut(s)	150
FaeI	CATG	5 cut(s)	162, 314, 616, 685, 957
FaqI	GGGAC	3 cut(s)	737, 972, 1032
FatI	CATG	5 cut(s)	158, 310, 612, 681, 953
FblI	GTMKAC	1 cut(s)	423
Fnu4HI	GCNGC	4 cut(s)	504, 598, 739, 900
FokI	GGATG	3 cut(s)	175, 728, 1003
FriOI	GRGCYC	1 cut(s)	150
Fsp4HI	GCNGC	4 cut(s)	504, 598, 739, 900
FspBI	CTAG	4 cut(s)	143, 335, 360, 884
GlaI	GCGC	1 cut(s)	442
GluI	GCNGC	4 cut(s)	504, 598, 739, 900
GsuI	CTGGAG	1 cut(s)	448
HaeII	RGCGCY	1 cut(s)	444
HaeIII	GGCC	1 cut(s)	1043
HapII	CCGG	2 cut(s)	113, 645
HhaI	GCGC	1 cut(s)	443
Hin1II	CATG	5 cut(s)	162, 314, 616, 685, 957
Hin6I	GCGC	1 cut(s)	441
HinP1I	GCGC	1 cut(s)	441
HindIII	AAGCTT	1 cut(s)	137
HinfI	GANTC	5 cut(s)	155, 232, 538, 1020, 1029
HpaII	CCGG	2 cut(s)	113, 645
HphI	GGTGA	1 cut(s)	790
Hpy166II	GTNNAC	3 cut(s)	208, 424, 1010
Hpy188I	TCNGA	6 cut(s)	169, 227, 237, 543, 556, 931
Hpy188III	TCNNGA	5 cut(s)	143, 360, 701, 825, 946
Hpy8I	GTNNAC	3 cut(s)	208, 424, 1010
HpyAV	CCTTC	3 cut(s)	196, 683, 775
HpyCH4III	ACNGT	5 cut(s)	272, 499, 575, 593, 893
HpyCH4IV	ACGT	1 cut(s)	724
HpyCH4V	TGCA	6 cut(s)	14, 208, 241, 344, 503, 600
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 53, 145, 556, 818
HpyF3I	CTNAG	2 cut(s)	376, 542
HpySE526I	ACGT	1 cut(s)	724
Hsp92II	CATG	5 cut(s)	162, 314, 616, 685, 957
HspAI	GCGC	1 cut(s)	441
Kzo9I	GATC	4 cut(s)	264, 516, 525, 926
LguI	GCTCTTC	1 cut(s)	155
LmnI	GCTCC	2 cut(s)	115, 454
Lsp1109I	GCAGC	3 cut(s)	515, 584, 911
LweI	GCATC	2 cut(s)	65, 228
MaeI	CTAG	4 cut(s)	143, 335, 360, 884
MaeII	ACGT	1 cut(s)	724
MaeIII	GTNAC	3 cut(s)	272, 575, 727
MalI	GATC	4 cut(s)	266, 518, 527, 928
MboI	GATC	4 cut(s)	264, 516, 525, 926
MboII	GAAGA	7 cut(s)	142, 326, 339, 674, 758, 840, 1006
MflI	RGATCY	1 cut(s)	926
MhlI	GDGCHC	2 cut(s)	150, 210
MluCI	AATT	4 cut(s)	29, 353, 636, 693
MlyI	GAGTC	2 cut(s)	164, 1014
MmeI	TCCRAC	2 cut(s)	82, 886
Mph1103I	ATGCAT	1 cut(s)	243
MseI	TTAA	1 cut(s)	861
MspA1I	CMGCKG	3 cut(s)	506, 738, 902
MspI	CCGG	2 cut(s)	113, 645
MspR9I	CCNGG	2 cut(s)	464, 776
Mva1269I	GAATGC	1 cut(s)	623
MvaI	CCWGG	2 cut(s)	464, 776
MwoI	GCNNNNNNNGC	5 cut(s)	11, 53, 145, 556, 818
NdeII	GATC	4 cut(s)	264, 516, 525, 926
NlaIII	CATG	5 cut(s)	162, 314, 616, 685, 957
NlaIV	GGNNCC	2 cut(s)	450, 960
NmuCI	GTSAC	2 cut(s)	575, 727
NsiI	ATGCAT	1 cut(s)	243
PciSI	GCTCTTC	1 cut(s)	155
PcsI	WCGNNNNNNNCGW	1 cut(s)	288
PctI	GAATGC	1 cut(s)	623
PfeI	GAWTC	3 cut(s)	232, 538, 1029
PflMI	CCANNNNNTGG	1 cut(s)	895
PinAI	ACCGGT	1 cut(s)	644
PkrI	GCNGC	4 cut(s)	505, 599, 740, 901
PleI	GAGTC	2 cut(s)	163, 1014
PpsI	GAGTC	2 cut(s)	163, 1014
PshAI	GACNNNNGTC	1 cut(s)	465
Psp124BI	GAGCTC	1 cut(s)	150
Psp6I	CCWGG	2 cut(s)	462, 774
PspGI	CCWGG	2 cut(s)	462, 774
PspN4I	GGNNCC	2 cut(s)	450, 960
PspPI	GGNCC	2 cut(s)	460, 959
PstNI	CAGNNNCTG	2 cut(s)	276, 597
PsuI	RGATCY	1 cut(s)	926
PvuII	CAGCTG	1 cut(s)	506
RsaI	GTAC	2 cut(s)	392, 632
RsaNI	GTAC	2 cut(s)	391, 631
SacI	GAGCTC	1 cut(s)	150
SapI	GCTCTTC	1 cut(s)	155
SaqAI	TTAA	1 cut(s)	861
SatI	GCNGC	4 cut(s)	504, 598, 739, 900
Sau3AI	GATC	4 cut(s)	264, 516, 525, 926
Sau96I	GGNCC	2 cut(s)	460, 959
SchI	GAGTC	2 cut(s)	164, 1014
ScrFI	CCNGG	2 cut(s)	464, 776
SduI	GDGCHC	2 cut(s)	150, 210
SfaNI	GCATC	2 cut(s)	65, 228
SinI	GGWCC	2 cut(s)	460, 959
Sse9I	AATT	4 cut(s)	29, 353, 636, 693
SsiI	CCGC	3 cut(s)	299, 738, 902
SspMI	CTAG	4 cut(s)	143, 335, 360, 884
SstI	GAGCTC	1 cut(s)	150
StyD4I	CCNGG	2 cut(s)	462, 774
TaaI	ACNGT	5 cut(s)	272, 499, 575, 593, 893
TaiI	ACGT	1 cut(s)	727
TaqI	TCGA	3 cut(s)	153, 510, 641
TasI	AATT	4 cut(s)	29, 353, 636, 693
TatI	WGTACW	1 cut(s)	630
TauI	GCSGC	1 cut(s)	741
TfiI	GAWTC	3 cut(s)	232, 538, 1029
Tru1I	TTAA	1 cut(s)	861
Tru9I	TTAA	1 cut(s)	861
TscAI	CASTG	4 cut(s)	136, 598, 811, 1009
TseFI	GTSAC	2 cut(s)	575, 727
TseI	GCWGC	3 cut(s)	503, 597, 899
Tsp45I	GTSAC	2 cut(s)	575, 727
TspDTI	ATGAA	3 cut(s)	327, 906, 1007
TspGWI	ACGGA	2 cut(s)	409, 419
TspRI	CASTG	4 cut(s)	136, 598, 811, 1009
Van91I	CCANNNNNTGG	1 cut(s)	895
VneI	GTGCAC	1 cut(s)	206
VpaK11BI	GGWCC	2 cut(s)	460, 959
XagI	CCTNNNNNAGG	1 cut(s)	779
XapI	RAATTY	2 cut(s)	29, 693
XbaI	TCTAGA	2 cut(s)	142, 359
XcmI	CCANNNNNNNNNTGG	1 cut(s)	470
XmiI	GTMKAC	1 cut(s)	423
XspI	CTAG	4 cut(s)	143, 335, 360, 884
Zsp2I	ATGCAT	1 cut(s)	243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.