Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006799

Physical Location & Seq

Genomic Coordinates Forward (+)

27159374 .. 27160814

Gene Structure 1441 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1141100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGGCTTTTTCGAAGATTTCGCTTCTCGCCTTCCTTGTGCCTCTCTTTCTCGGTTCTACTCTTGCTCACGAGTTCTCGATCGTGGGATATTCTCCCGAGGACTTGACCTGCATGGACAAGTTCATCATGCTCTTCGAGAAGTGGATATCGAAGCACGGAAAGATTTATGAGAGCATGGAGGAGAAGCTGCATAGATTTGAGATTTTCAGAGACAATGTGAAGCACATTGATGAGAGGAACAAGAACCTCGATGTCGATAGCTACTGGCTTGCTGTGAATGAGTTTGCTGACTTGAGCCATGAGGAGTTCAAGAGCAAGTACTTGGGTCTCAAGGCTGAGTTCCCCAGAAGGAGAGATTCCACTGGAGAGTTCAGTTACCGCGATTTTGATGTAGAGGCATTGCCCAAATCCGTGGACTGGCGAAAGAAAGGAGCTGTAACCTCCATCAAGAACCAAGGTTCATGTGGTAGCTGTTGGGCATTTTCCACTGTTGCTGCAGTTGAGGGCATAAACCAGATAGTCACAGGAAGCCTAACATCTCTGTCTGAGCAGCAGCTGATAGATTGTGACAAATCATTCAACAATGGCTGCAATGGAGGCTTGATGGATTATGCATTCGAGTTCATAATCTCCAACGGTGGCCTCCACAAGGAGGATGATTACCCCTACATTATGGACGAAGGAACTTGTGCGGAAAAGAAGGCAGACTCGGACGTAGTGACTATCTCCGGTTACCATGACGTGCCGGAAAACAACGAGCAGGCTCTCCTCAAGGCACTCGCAAACCAGCCTCTCAGCGTAGCCATTGAGGCCTCCAGCAGAGATTTCCAGTTCTATAGCGGGGGTGTCTTCAACGGGCGTTGCGGAACTGAACTAGACCACGGTGTAGCAGCCGTCGGATATGGGACTCAGGATGGGTTAGATTACATCATTGTGAAGAACTCATGGGGGCCAAAGTGGGGAGAAAAGGGATACATAAGGATGAAGAGGAGCACAGGGAAACCAGAAGGAATCTGTGGCATTAACAAGATGGCTTCATATCCTACCAAAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000325 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0010623 GO:0012501 GO:0016787 GO:0019538 GO:0030154 GO:0030163 GO:0032502 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

353

Amino Acids

39.42

Weight (kDa)

5.49

Isoelectric Point (pI)

28.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	45 - 103	1.1e-18	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	134 - 349	3.5e-84	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006799)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20850 AT4G35350 AT4G35350
fragaria_vesca	FvH4_2g16940
malus_domestica	MD05G1141100.v1.1 MD10G1142500.v1.1 MD11G1242400.v1.1
prunus_persica	Prupe.8G181100_v2.0.a1
pyrus_communis	pycom05g13650
rosa_chinensis	RchiOBHm_Chr6g0281081 RchiOBHm_Chr6g0281131
rosa_laevigata	RLG00000012992 RLG00000012998
rosa_multiflora	Rmu_co8502169.1_g000002 Rmu_sc0001705.1_g000005 Rmu_sc0001705.1_g000018
rosa_roxburghii	Rroxscaffold_7G00187270 Rroxscaffold_7G00187300 Rroxscaffold_7G00187320
rosa_rugosa	Rorug06G0138900 Rorug06G0139000 Rorug06G0139100
rosa_samantha	Rh6BG253900 Rh6BG254100
rosa_wichuraiana	Rw6G021770 Rw6G021780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	116
AccII	CGCG	1 cut(s)	381
AciI	CCGC	4 cut(s)	379, 692, 840, 864
AfaI	GTAC	1 cut(s)	320
AfiI	CCNNNNNNNGG	4 cut(s)	417, 649, 652, 959
AgsI	TTSAA	3 cut(s)	310, 580, 853
AjiI	CACGTC	1 cut(s)	742
AluBI	AGCT	5 cut(s)	187, 261, 434, 471, 556
AluI	AGCT	5 cut(s)	187, 261, 434, 471, 556
Alw21I	GWGCWC	1 cut(s)	995
Alw26I	GTCTC	2 cut(s)	204, 332
AlwNI	CAGNNNCTG	1 cut(s)	556
Ama87I	CYCGRG	1 cut(s)	95
AoxI	GGCC	3 cut(s)	640, 810, 950
ApeKI	GCWGC	6 cut(s)	187, 494, 550, 553, 588, 890
AspS9I	GGNCC	1 cut(s)	950
AsuII	TTCGAA	1 cut(s)	11
AvaI	CYCGRG	1 cut(s)	95
BauI	CACGAG	1 cut(s)	68
BbsI	GAAGAC	1 cut(s)	841
Bbv12I	GWGCWC	1 cut(s)	995
BbvI	GCAGC	6 cut(s)	174, 481, 562, 565, 575, 902
BccI	CCATC	4 cut(s)	452, 598, 908, 1024
BceAI	ACGGC	1 cut(s)	878
BciVI	GTATCC	1 cut(s)	965
BcoDI	GTCTC	2 cut(s)	204, 332
BfaI	CTAG	1 cut(s)	875
BfmI	CTRYAG	2 cut(s)	495, 835
BfuAI	ACCTGC	1 cut(s)	116
BfuI	GTATCC	1 cut(s)	965
BglI	GCCNNNNNGGC	1 cut(s)	809
BisI	GCNGC	6 cut(s)	188, 495, 551, 554, 589, 891
BlsI	GCNGC	6 cut(s)	189, 496, 552, 555, 590, 892
BmcAI	AGTACT	1 cut(s)	320
BmeT110I	CYCGRG	1 cut(s)	95
BmgBI	CACGTC	1 cut(s)	742
BmgT120I	GGNCC	1 cut(s)	950
BmiI	GGNNCC	1 cut(s)	951
BpiI	GAAGAC	1 cut(s)	841
BpmI	CTGGAG	2 cut(s)	384, 799
Bpu14I	TTCGAA	1 cut(s)	11
BpuEI	CTTGAG	3 cut(s)	313, 314, 755
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	4 cut(s)	96, 411, 454, 880
BsaWI	WCCGGW	1 cut(s)	728
Bsc4I	CCNNNNNNNGG	4 cut(s)	417, 649, 652, 959
Bse1I	ACTGG	4 cut(s)	269, 367, 422, 829
Bse3DI	GCAATG	2 cut(s)	398, 598
BseDI	CCNNGG	4 cut(s)	96, 411, 454, 880
BseGI	GGATG	3 cut(s)	661, 919, 987
BseLI	CCNNNNNNNGG	4 cut(s)	417, 649, 652, 959
BseMI	GCAATG	2 cut(s)	398, 598
BseMII	CTCAG	4 cut(s)	327, 537, 808, 923
BseNI	ACTGG	4 cut(s)	269, 367, 422, 829
BseRI	GAGGAG	4 cut(s)	194, 317, 758, 1003
BseXI	GCAGC	6 cut(s)	174, 481, 562, 565, 575, 902
Bsh1236I	CGCG	1 cut(s)	381
Bsh1285I	CGRYCG	1 cut(s)	81
BshFI	GGCC	3 cut(s)	642, 812, 952
BsiEI	CGRYCG	1 cut(s)	81
BsiHKAI	GWGCWC	1 cut(s)	995
BsiHKCI	CYCGRG	1 cut(s)	95
BsiSI	CCGG	2 cut(s)	729, 746
BslFI	GGGAC	1 cut(s)	919
BslI	CCNNNNNNNGG	4 cut(s)	417, 649, 652, 959
BsmAI	GTCTC	2 cut(s)	204, 332
BsmFI	GGGAC	1 cut(s)	919
BsmI	GAATGC	1 cut(s)	614
BsnI	GGCC	3 cut(s)	642, 812, 952
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	1 cut(s)	95
Bsp119I	TTCGAA	1 cut(s)	11
Bsp1286I	GDGCHC	1 cut(s)	995
Bsp143I	GATC	1 cut(s)	78
BspACI	CCGC	4 cut(s)	379, 692, 840, 864
BspANI	GGCC	3 cut(s)	642, 812, 952
BspCNI	CTCAG	4 cut(s)	328, 538, 807, 922
BspFNI	CGCG	1 cut(s)	381
BspLI	GGNNCC	1 cut(s)	951
BspMAI	CTGCAG	1 cut(s)	499
BspMI	ACCTGC	1 cut(s)	116
BspQI	GCTCTTC	1 cut(s)	137
BspT104I	TTCGAA	1 cut(s)	11
BspTNI	GGTCTC	1 cut(s)	332
BsrDI	GCAATG	2 cut(s)	398, 598
BsrI	ACTGG	4 cut(s)	269, 367, 422, 829
BssECI	CCNNGG	4 cut(s)	96, 411, 454, 880
BssMI	GATC	1 cut(s)	78
BssSI	CACGAG	1 cut(s)	68
BssT1I	CCWWGG	1 cut(s)	454
Bst2BI	CACGAG	1 cut(s)	68
Bst4CI	ACNGT	3 cut(s)	490, 638, 884
Bst6I	CTCTTC	2 cut(s)	137, 980
BstBI	TTCGAA	1 cut(s)	11
BstC8I	GCNNGC	2 cut(s)	270, 762
BstDEI	CTNAG	4 cut(s)	336, 546, 794, 909
BstDSI	CCRYGG	2 cut(s)	411, 880
BstEII	GGTNACC	1 cut(s)	731
BstENI	CCTNNNNNAGG	1 cut(s)	647
BstF5I	GGATG	3 cut(s)	661, 919, 987
BstFNI	CGCG	1 cut(s)	381
BstKTI	GATC	1 cut(s)	81
BstMAI	GTCTC	2 cut(s)	204, 332
BstMBI	GATC	1 cut(s)	78
BstMCI	CGRYCG	1 cut(s)	81
BstMWI	GCNNNNNNNGC	2 cut(s)	597, 809
BstPI	GGTNACC	1 cut(s)	731
BstSFI	CTRYAG	2 cut(s)	495, 835
BstUI	CGCG	1 cut(s)	381
BstV1I	GCAGC	6 cut(s)	174, 481, 562, 565, 575, 902
BstV2I	GAAGAC	1 cut(s)	841
BstXI	CCANNNNNNTGG	1 cut(s)	412
BsuI	GTATCC	1 cut(s)	965
BsuRI	GGCC	3 cut(s)	642, 812, 952
BtgI	CCRYGG	2 cut(s)	411, 880
BtrI	CACGTC	1 cut(s)	742
BtsCI	GGATG	3 cut(s)	661, 919, 987
BtsIMutI	CAGTG	2 cut(s)	360, 486
BveI	ACCTGC	1 cut(s)	116
Cac8I	GCNNGC	2 cut(s)	270, 762
CaiI	CAGNNNCTG	1 cut(s)	556
Cfr13I	GGNCC	1 cut(s)	950
Csp6I	GTAC	1 cut(s)	319
CviAII	CATG	7 cut(s)	112, 127, 175, 299, 462, 737, 945
CviQI	GTAC	1 cut(s)	319
DdeI	CTNAG	4 cut(s)	336, 546, 794, 909
DpnI	GATC	1 cut(s)	80
DpnII	GATC	1 cut(s)	78
Eam1104I	CTCTTC	2 cut(s)	137, 980
EarI	CTCTTC	2 cut(s)	137, 980
Eco130I	CCWWGG	1 cut(s)	454
Eco147I	AGGCCT	1 cut(s)	812
Eco31I	GGTCTC	1 cut(s)	332
Eco32I	GATATC	1 cut(s)	147
Eco88I	CYCGRG	1 cut(s)	95
Eco91I	GGTNACC	1 cut(s)	731
EcoNI	CCTNNNNNAGG	1 cut(s)	647
EcoO65I	GGTNACC	1 cut(s)	731
EcoRV	GATATC	1 cut(s)	147
EcoT14I	CCWWGG	1 cut(s)	454
EcoT22I	ATGCAT	1 cut(s)	616
ErhI	CCWWGG	1 cut(s)	454
FaeI	CATG	7 cut(s)	115, 130, 178, 302, 465, 740, 948
FaqI	GGGAC	1 cut(s)	919
FatI	CATG	7 cut(s)	111, 126, 174, 298, 461, 736, 944
FauI	CCCGC	1 cut(s)	833
Fnu4HI	GCNGC	6 cut(s)	188, 495, 551, 554, 589, 891
FokI	GGATG	3 cut(s)	668, 926, 994
Fsp4HI	GCNGC	6 cut(s)	188, 495, 551, 554, 589, 891
FspBI	CTAG	1 cut(s)	875
GluI	GCNGC	6 cut(s)	188, 495, 551, 554, 589, 891
GsuI	CTGGAG	2 cut(s)	384, 799
HaeIII	GGCC	3 cut(s)	642, 812, 952
HapII	CCGG	2 cut(s)	729, 746
Hin1II	CATG	7 cut(s)	115, 130, 178, 302, 465, 740, 948
HinfI	GANTC	4 cut(s)	356, 707, 907, 1011
HpaII	CCGG	2 cut(s)	729, 746
Hpy166II	GTNNAC	1 cut(s)	415
Hpy188I	TCNGA	4 cut(s)	209, 547, 712, 899
Hpy188III	TCNNGA	7 cut(s)	68, 76, 95, 136, 310, 448, 911
Hpy8I	GTNNAC	1 cut(s)	415
Hpy99I	CGWCG	1 cut(s)	899
HpyAV	CCTTC	5 cut(s)	40, 342, 674, 694, 1001
HpyCH4III	ACNGT	3 cut(s)	490, 638, 884
HpyCH4IV	ACGT	2 cut(s)	714, 741
HpyCH4V	TGCA	5 cut(s)	111, 190, 497, 591, 614
HpyF10VI	GCNNNNNNNGC	2 cut(s)	597, 809
HpyF3I	CTNAG	4 cut(s)	336, 546, 794, 909
HpySE526I	ACGT	2 cut(s)	714, 741
Hsp92II	CATG	7 cut(s)	115, 130, 178, 302, 465, 740, 948
Kzo9I	GATC	1 cut(s)	78
LguI	GCTCTTC	1 cut(s)	137
LmnI	GCTCC	2 cut(s)	431, 990
Lsp1109I	GCAGC	6 cut(s)	174, 481, 562, 565, 575, 902
MaeI	CTAG	1 cut(s)	875
MaeII	ACGT	2 cut(s)	714, 741
MaeIII	GTNAC	6 cut(s)	374, 436, 520, 566, 718, 731
MalI	GATC	1 cut(s)	80
MboI	GATC	1 cut(s)	78
MboII	GAAGA	5 cut(s)	25, 124, 841, 949, 997
MhlI	GDGCHC	1 cut(s)	995
MlyI	GAGTC	2 cut(s)	701, 901
MmeI	TCCRAC	2 cut(s)	657, 877
Mph1103I	ATGCAT	1 cut(s)	616
MseI	TTAA	1 cut(s)	1023
MslI	CAYNNNNRTG	3 cut(s)	228, 932, 980
MspA1I	CMGCKG	1 cut(s)	556
MspI	CCGG	2 cut(s)	729, 746
Mva1269I	GAATGC	1 cut(s)	614
MvnI	CGCG	1 cut(s)	381
MwoI	GCNNNNNNNGC	2 cut(s)	597, 809
NdeII	GATC	1 cut(s)	78
NlaIII	CATG	7 cut(s)	115, 130, 178, 302, 465, 740, 948
NlaIV	GGNNCC	1 cut(s)	951
NmuCI	GTSAC	3 cut(s)	520, 566, 718
NsiI	ATGCAT	1 cut(s)	616
NspV	TTCGAA	1 cut(s)	11
PceI	AGGCCT	1 cut(s)	812
PciSI	GCTCTTC	1 cut(s)	137
PctI	GAATGC	1 cut(s)	614
PfeI	GAWTC	2 cut(s)	356, 1011
PkrI	GCNGC	6 cut(s)	189, 496, 552, 555, 590, 892
Ple19I	CGATCG	1 cut(s)	81
PleI	GAGTC	2 cut(s)	701, 901
PpsI	GAGTC	2 cut(s)	701, 901
PspEI	GGTNACC	1 cut(s)	731
PspN4I	GGNNCC	1 cut(s)	951
PspPI	GGNCC	1 cut(s)	950
PstI	CTGCAG	1 cut(s)	499
PstNI	CAGNNNCTG	1 cut(s)	556
PvuI	CGATCG	1 cut(s)	81
PvuII	CAGCTG	1 cut(s)	556
RsaI	GTAC	1 cut(s)	320
RsaNI	GTAC	1 cut(s)	319
RseI	CAYNNNNRTG	3 cut(s)	228, 932, 980
SapI	GCTCTTC	1 cut(s)	137
SaqAI	TTAA	1 cut(s)	1023
SatI	GCNGC	6 cut(s)	188, 495, 551, 554, 589, 891
Sau3AI	GATC	1 cut(s)	78
Sau96I	GGNCC	1 cut(s)	950
ScaI	AGTACT	1 cut(s)	320
SchI	GAGTC	2 cut(s)	701, 901
SduI	GDGCHC	1 cut(s)	995
SfcI	CTRYAG	2 cut(s)	495, 835
SfuI	TTCGAA	1 cut(s)	11
SmiMI	CAYNNNNRTG	3 cut(s)	228, 932, 980
SmlI	CTYRAG	3 cut(s)	292, 329, 770
SmoI	CTYRAG	3 cut(s)	292, 329, 770
SseBI	AGGCCT	1 cut(s)	812
SsiI	CCGC	4 cut(s)	379, 692, 840, 864
SspMI	CTAG	1 cut(s)	875
StuI	AGGCCT	1 cut(s)	812
StyI	CCWWGG	1 cut(s)	454
TaaI	ACNGT	3 cut(s)	490, 638, 884
TaiI	ACGT	2 cut(s)	717, 744
TaqI	TCGA	7 cut(s)	11, 77, 135, 149, 249, 255, 618
TatI	WGTACW	1 cut(s)	318
TfiI	GAWTC	2 cut(s)	356, 1011
Tru1I	TTAA	1 cut(s)	1023
Tru9I	TTAA	1 cut(s)	1023
TscAI	CASTG	2 cut(s)	367, 493
TseFI	GTSAC	3 cut(s)	520, 566, 718
TseI	GCWGC	6 cut(s)	187, 494, 550, 553, 588, 890
Tsp45I	GTSAC	3 cut(s)	520, 566, 718
TspDTI	ATGAA	5 cut(s)	112, 450, 613, 998, 1026
TspGWI	ACGGA	2 cut(s)	171, 400
TspRI	CASTG	2 cut(s)	367, 493
XagI	CCTNNNNNAGG	1 cut(s)	647
XcmI	CCANNNNNNNNNTGG	1 cut(s)	461
XspI	CTAG	1 cut(s)	875
ZrmI	AGTACT	1 cut(s)	320
Zsp2I	ATGCAT	1 cut(s)	616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.