AT4G35940

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006081
Physical Location & Seq
Forward (+)
17021380 .. 17024108
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35940.2

Sequence Viewer

Length: 1356 bp
ATGTCTCGGTGTTTTCCGTTCCCGCCACCTGGTTATGTACTAAACGGAATCCGCGATGAGGCTGTGATCGTATCATCGATCAAGGGGGTGGAGGAGAAAGCTAAGAAAGAACAAAGGAGGAAGGATAGAAGAAGTGATAAGAAGGATAAAAAAGATAAGAAGGAGAGGAAGGAGAAGAAGGAGAAGAAGGAGAAAAAGAGGAAGGAGAGGGAAGGAAAGGAAGTTGGAAGCGAAAAGCGCAGTCATAAGAGACGGCGTAAAGAAGACGGTGCTAAAGTTGATCTTTTTCACAAACTAAAAGAAAGTGAAGTCAATTGTTTGGAGAAGAGCAGCCTTACTGTAGAACGTGAGCTGCTTCAGTCGACGTCCCAAAACTCTTGTGATAGCACTCTTAACAGCAATGAGATGTTACCGAAGCAGAAGGAGGTCCAGCAGCCTCTCGATGGTAGACATAACAACAACAACAACGAGAAGCGTGTGGAGAAGCAGCAGCCTCTCGATGGTAGACATAACAACAACAACGAGAAGCGTGTGGAGAAGCAGCAGCCTCTCGATGGTAGACATAACAACAACAACGAGAAGCGGATTGAGAAGCAGCAGCCTCTCAATGGTAGACATAACAACAACAACGAAAAGCTGATGGAGAAGCAGCAGCCTCTCAATGGTAGACATAACAACAACAACGAGAAGCGGATTGAGAAGCAGCAGCCTCTCAATGGTAGACATAACAACAAGGAGAAGCAGAAAGAGAAACAGCAGCCTCTCGATGTTAGACATAACAACAACGACTCTGAAAGCATCATTCGGATTCGATTGCCTATTCGAAGGCAGAAAGATCCCGAGGTGATGATGACCAATAAGGATCAAGAAAAACCAGGTCCTTCTCGGGGAATAAAGTTAGACTCGTCCCAACTTCCTACTAGAGAGCCGGTTAATCAACATCCTTGTTCCACTTCTGCAGCAGAACATGCTTCGAAGCCACGAGAAGAGAAAAGGAAAGATCCCATTTTTAGAGGGAAACACGGTAAAGAGAAGATATCCTCATCCTCTACTCGAGAAACTTATCAACCTCCTAAAAGCCTATGCAATTGCCCTCCATCCATGGTGTTGCAATTCTTGGACGTAGTTGAGAATTGGGTCCCTAACACGATCGAGAGAAGAGTAGACCTCATTAACTCTGAAGATGAAGAATGTTGGTGGTCCATGAAGAAGCCGCCAAGTAGCACCACAGAAATATGCAAACAACTTAATAGAGAGAATGAGATCAAGCAAGTAGGCAATACAATGGGGTGGCCATGTGCTCGCCTTTTACCAGAAGCTGATGTCTACGCCTTACCTTACACAGTCCCGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

451

Amino Acids

52.72

Weight (kDa)

9.6

Isoelectric Point (pI)

69.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
AccI GTMKAC 9 cut(s) 362, 448, 505, 559, 613, 667, 721, 1164, 1326
AccII CGCG 1 cut(s) 54
AciI CCGC 5 cut(s) 23, 52, 583, 691, 1214
AclWI GGATC 3 cut(s) 830, 870, 995
AcoI YGGCCR 1 cut(s) 1292
AcuI CTGAAG 2 cut(s) 341, 1200
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 9 cut(s) 29, 58, 443, 500, 554, 608, 662, 716, 887
AjnI CCWGG 2 cut(s) 28, 874
AluBI AGCT 4 cut(s) 101, 352, 637, 1319
AluI AGCT 4 cut(s) 101, 352, 637, 1319
Alw21I GWGCWC 1 cut(s) 1303
Alw26I GTCTC 2 cut(s) 9, 244
AlwI GGATC 3 cut(s) 830, 870, 995
AlwNI CAGNNNCTG 1 cut(s) 1319
Ama87I CYCGRG 3 cut(s) 839, 885, 1053
AoxI GGCC 1 cut(s) 1292
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 4 cut(s) 427, 878, 1138, 1200
AsuHPI GGTGA 1 cut(s) 856
AsuII TTCGAA 2 cut(s) 823, 974
AvaI CYCGRG 3 cut(s) 839, 885, 1053
AvaII GGWCC 4 cut(s) 427, 878, 1138, 1200
BalI TGGCCA 1 cut(s) 1294
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 1303
BccI CCATC 5 cut(s) 437, 494, 548, 634, 1105
BceAI ACGGC 1 cut(s) 269
BciT130I CCWGG 2 cut(s) 30, 876
BcoDI GTCTC 2 cut(s) 9, 244
BfaI CTAG 1 cut(s) 921
BfmI CTRYAG 2 cut(s) 339, 957
Bme1390I CCNGG 2 cut(s) 30, 876
Bme18I GGWCC 4 cut(s) 427, 878, 1138, 1200
BmeT110I CYCGRG 3 cut(s) 839, 885, 1053
BmgT120I GGNCC 4 cut(s) 427, 878, 1138, 1200
BmiI GGNNCC 2 cut(s) 1139, 1140
BmrFI CCNGG 2 cut(s) 30, 876
BmsI GCATC 1 cut(s) 807
BpiI GAAGAC 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 1152, 1184
Bpu14I TTCGAA 2 cut(s) 823, 974
Bsa29I ATCGAT 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 840, 1101
Bsc4I CCNNNNNNNGG 9 cut(s) 29, 58, 443, 500, 554, 608, 662, 716, 887
Bse118I RCCGGY 1 cut(s) 928
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 2 cut(s) 30, 876
BseCI ATCGAT 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 840, 1101
BseGI GGATG 3 cut(s) 940, 1043, 1097
BseLI CCNNNNNNNGG 9 cut(s) 29, 58, 443, 500, 554, 608, 662, 716, 887
BseMI GCAATG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 1 cut(s) 1152
BshFI GGCC 1 cut(s) 1294
BshVI ATCGAT 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 1152
BsiHKAI GWGCWC 1 cut(s) 1303
BsiHKCI CYCGRG 3 cut(s) 839, 885, 1053
BsiSI CCGG 1 cut(s) 929
BslFI GGGAC 4 cut(s) 352, 892, 1124, 1331
BslI CCNNNNNNNGG 9 cut(s) 29, 58, 443, 500, 554, 608, 662, 716, 887
BsmAI GTCTC 2 cut(s) 9, 244
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 4 cut(s) 352, 892, 1124, 1331
BsnI GGCC 1 cut(s) 1294
BsoBI CYCGRG 3 cut(s) 839, 885, 1053
Bsp119I TTCGAA 2 cut(s) 823, 974
Bsp1286I GDGCHC 1 cut(s) 1303
Bsp143I GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
Bsp19I CCATGG 1 cut(s) 1101
BspACI CCGC 5 cut(s) 23, 52, 583, 691, 1214
BspANI GGCC 1 cut(s) 1294
BspDI ATCGAT 1 cut(s) 77
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 1139, 1140
BspMAI CTGCAG 1 cut(s) 961
BspPI GGATC 3 cut(s) 830, 870, 995
BspQI GCTCTTC 1 cut(s) 320
BspT104I TTCGAA 2 cut(s) 823, 974
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 928
BssAI RCCGGY 1 cut(s) 928
BssECI CCNNGG 2 cut(s) 840, 1101
BssMI GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 1 cut(s) 1101
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 30, 876
Bst4CI ACNGT 4 cut(s) 269, 340, 1025, 1345
Bst6I CTCTTC 3 cut(s) 320, 981, 1153
BstACI GRCGYC 1 cut(s) 365
BstAPI GCANNNNNTGC 1 cut(s) 968
BstBI TTCGAA 2 cut(s) 823, 974
BstC8I GCNNGC 1 cut(s) 1303
BstDEI CTNAG 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 1101
BstF5I GGATG 3 cut(s) 940, 1043, 1097
BstFNI CGCG 1 cut(s) 54
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 8 cut(s) 69, 81, 283, 838, 865, 1003, 1152, 1266
BstMAI GTCTC 2 cut(s) 9, 244
BstMBI GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
BstMCI CGRYCG 1 cut(s) 1152
BstMWI GCNNNNNNNGC 2 cut(s) 237, 968
BstNI CCWGG 2 cut(s) 30, 876
BstNSI RCATGY 1 cut(s) 971
BstSCI CCNGG 2 cut(s) 28, 874
BstSFI CTRYAG 2 cut(s) 339, 957
BstUI CGCG 1 cut(s) 54
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 2 cut(s) 835, 1000
BstYI RGATCY 2 cut(s) 835, 1000
Bsu15I ATCGAT 1 cut(s) 77
BsuRI GGCC 1 cut(s) 1294
BsuTUI ATCGAT 1 cut(s) 77
BtgI CCRYGG 1 cut(s) 1101
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 3 cut(s) 940, 1043, 1097
Cac8I GCNNGC 1 cut(s) 1303
CaiI CAGNNNCTG 1 cut(s) 1319
CfoI GCGC 1 cut(s) 240
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 4 cut(s) 427, 878, 1138, 1200
ClaI ATCGAT 1 cut(s) 77
CsiI ACCWGGT 2 cut(s) 28, 874
Csp6I GTAC 1 cut(s) 38
CviAII CATG 4 cut(s) 968, 1102, 1204, 1296
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 1 cut(s) 102
DpnI GATC 8 cut(s) 68, 80, 282, 837, 864, 1002, 1151, 1265
DpnII GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
EaeI YGGCCR 1 cut(s) 1292
Eam1104I CTCTTC 3 cut(s) 320, 981, 1153
EarI CTCTTC 3 cut(s) 320, 981, 1153
Eco130I CCWWGG 1 cut(s) 1101
Eco32I GATATC 1 cut(s) 1038
Eco47I GGWCC 4 cut(s) 427, 878, 1138, 1200
Eco57I CTGAAG 2 cut(s) 341, 1200
Eco88I CYCGRG 3 cut(s) 839, 885, 1053
EcoO109I RGGNCCY 2 cut(s) 878, 1138
EcoRII CCWGG 2 cut(s) 28, 874
EcoRV GATATC 1 cut(s) 1038
EcoT14I CCWWGG 1 cut(s) 1101
ErhI CCWWGG 1 cut(s) 1101
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 4 cut(s) 971, 1105, 1207, 1299
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 4 cut(s) 352, 892, 1124, 1331
FatI CATG 4 cut(s) 967, 1101, 1203, 1295
FauI CCCGC 1 cut(s) 30
FblI GTMKAC 9 cut(s) 362, 448, 505, 559, 613, 667, 721, 1164, 1326
FokI GGATG 3 cut(s) 927, 1030, 1084
FspBI CTAG 1 cut(s) 921
GlaI GCGC 1 cut(s) 239
HaeIII GGCC 1 cut(s) 1294
HapII CCGG 1 cut(s) 929
HhaI GCGC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 4 cut(s) 971, 1105, 1207, 1299
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 4 cut(s) 48, 788, 808, 902
HpaII CCGG 1 cut(s) 929
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 9 cut(s) 363, 449, 506, 560, 614, 668, 722, 1165, 1327
Hpy188I TCNGA 4 cut(s) 793, 807, 1180, 1355
Hpy188III TCNNGA 8 cut(s) 440, 497, 551, 764, 839, 866, 1055, 1153
Hpy8I GTNNAC 9 cut(s) 363, 449, 506, 560, 614, 668, 722, 1165, 1327
Hpy99I CGWCG 1 cut(s) 367
HpyCH4III ACNGT 4 cut(s) 269, 340, 1025, 1345
HpyCH4IV ACGT 3 cut(s) 346, 365, 1122
HpyCH4V TGCA 4 cut(s) 959, 1086, 1111, 1239
HpyF10VI GCNNNNNNNGC 2 cut(s) 237, 968
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 3 cut(s) 346, 365, 1122
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 4 cut(s) 971, 1105, 1207, 1299
HspAI GCGC 1 cut(s) 238
KflI GGGWCCC 1 cut(s) 1138
Kzo9I GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
LguI GCTCTTC 1 cut(s) 320
LpnPI CCDG 7 cut(s) 15, 42, 443, 861, 888, 942, 1326
LweI GCATC 1 cut(s) 807
MabI ACCWGGT 2 cut(s) 28, 874
MaeI CTAG 1 cut(s) 921
MaeII ACGT 3 cut(s) 346, 365, 1122
MaeIII GTNAC 1 cut(s) 408
MalI GATC 8 cut(s) 68, 80, 282, 837, 864, 1002, 1151, 1265
MboI GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
MfeI CAATTG 2 cut(s) 313, 1087
MflI RGATCY 2 cut(s) 835, 1000
MhlI GDGCHC 1 cut(s) 1303
MlsI TGGCCA 1 cut(s) 1294
MluCI AATT 4 cut(s) 313, 1087, 1112, 1132
MluNI TGGCCA 1 cut(s) 1294
MlyI GAGTC 2 cut(s) 782, 896
MmeI TCCRAC 1 cut(s) 205
Mox20I TGGCCA 1 cut(s) 1294
MscI TGGCCA 1 cut(s) 1294
MseI TTAA 4 cut(s) 393, 933, 1173, 1248
Msp20I TGGCCA 1 cut(s) 1294
MspI CCGG 1 cut(s) 929
MspR9I CCNGG 2 cut(s) 30, 876
MunI CAATTG 2 cut(s) 313, 1087
MvaI CCWGG 2 cut(s) 30, 876
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 237, 968
NcoI CCATGG 1 cut(s) 1101
NdeII GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
NlaIII CATG 4 cut(s) 971, 1105, 1207, 1299
NlaIV GGNNCC 2 cut(s) 1139, 1140
NspI RCATGY 1 cut(s) 971
NspV TTCGAA 2 cut(s) 823, 974
PaeR7I CTCGAG 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 2 cut(s) 48, 808
Ple19I CGATCG 1 cut(s) 1152
PleI GAGTC 2 cut(s) 782, 896
PpsI GAGTC 2 cut(s) 782, 896
PpuMI RGGWCCY 2 cut(s) 878, 1138
Psp5II RGGWCCY 2 cut(s) 878, 1138
Psp6I CCWGG 2 cut(s) 28, 874
PspGI CCWGG 2 cut(s) 28, 874
PspN4I GGNNCC 2 cut(s) 1139, 1140
PspPI GGNCC 4 cut(s) 427, 878, 1138, 1200
PspPPI RGGWCCY 2 cut(s) 878, 1138
PstI CTGCAG 1 cut(s) 961
PstNI CAGNNNCTG 1 cut(s) 1319
PsuI RGATCY 2 cut(s) 835, 1000
PvuI CGATCG 1 cut(s) 1152
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SalI GTCGAC 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 4 cut(s) 393, 933, 1173, 1248
Sau3AI GATC 8 cut(s) 66, 78, 280, 835, 862, 1000, 1149, 1263
Sau96I GGNCC 4 cut(s) 427, 878, 1138, 1200
SchI GAGTC 2 cut(s) 782, 896
ScrFI CCNGG 2 cut(s) 30, 876
SduI GDGCHC 1 cut(s) 1303
SexAI ACCWGGT 2 cut(s) 28, 874
SfaNI GCATC 1 cut(s) 807
SfcI CTRYAG 2 cut(s) 339, 957
Sfr274I CTCGAG 1 cut(s) 1053
SfuI TTCGAA 2 cut(s) 823, 974
SinI GGWCC 4 cut(s) 427, 878, 1138, 1200
SlaI CTCGAG 1 cut(s) 1053
SmlI CTYRAG 1 cut(s) 1053
SmoI CTYRAG 1 cut(s) 1053
Sse9I AATT 4 cut(s) 313, 1087, 1112, 1132
SsiI CCGC 5 cut(s) 23, 52, 583, 691, 1214
SspMI CTAG 1 cut(s) 921
StyD4I CCNGG 2 cut(s) 28, 874
StyI CCWWGG 1 cut(s) 1101
TaaI ACNGT 4 cut(s) 269, 340, 1025, 1345
TaiI ACGT 3 cut(s) 349, 368, 1125
TasI AATT 4 cut(s) 313, 1087, 1112, 1132
TatI WGTACW 1 cut(s) 37
TauI GCSGC 1 cut(s) 1216
TfiI GAWTC 2 cut(s) 48, 808
Tru1I TTAA 4 cut(s) 393, 933, 1173, 1248
Tru9I TTAA 4 cut(s) 393, 933, 1173, 1248
TspDTI ATGAA 2 cut(s) 1200, 1220
TspGWI ACGGA 2 cut(s) 6, 60
VpaK11BI GGWCC 4 cut(s) 427, 878, 1138, 1200
XceI RCATGY 1 cut(s) 971
XhoI CTCGAG 1 cut(s) 1053
XmiI GTMKAC 9 cut(s) 362, 448, 505, 559, 613, 667, 721, 1164, 1326
XspI CTAG 1 cut(s) 921
ZraI GACGTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.