AT4G39420

phagosome-lysosome fusion involved in apoptotic cell clearance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003216
Physical Location & Seq
Forward (+)
18339127 .. 18355149
16023 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G39420.4

Sequence Viewer

Length: 7542 bp
ATGGAAAAGTTGGTGAAGGAAGGCCCAACTCTGCTGCAACTGCACAAGTGGGAACCTTCTCAATTTCAACTCAAGCTATCAGAATTTCGCGAGGCGTTTATTTCTCCGTCGAGGCAGTTGTTATTACTACTCTCTTACCACTCCGAAGCTTTACTGCTTCCTTTGGTTGCAGGGAGGTCCATTGGCAGTGAGGTTTCGTTAAGTGGTGATAATGAAGAGTTGAATAGTCCTTCATGCTCTGGTGGGTCAGATCCAGAGAAAATTGAATCTCCTTGTGGATCAGGAGTTGGTTCTGGTTGGGACCTCAAAATTGTTCGTTTGCGTCGACTGCAAATGGCGTTGGACTACTTAAAATATGATGACATCAACGAGTCTTTGAAGATGCTTGGTAATGTGAAGCTGGCAGAGGAAGGCATGCTGAGGGTGCTCTTTTCTGCTGTGTATCTATTGTCGCGCAAGGATAGAAACGATAATGAGATTTCTGCTGTATCTAGGCTCCTTGGATTGGCTACAATGTTTGCGACTGAGATGATTCGCAGATATGGGTTACTTGAATATCGGAAAGATGTTTACATGTTTGACAGCAAACCTAGGACACAGATACTTTCACTTCCAGCAGTTTCACTTAATATCGATGTAATGGAAAATTCAAGGAGGCTGTCAGAGATGGGTTATCTATTGGAAATCACCAGGAATATTCAATCCCGTATTACCAGAAAGTTTAAGAAGCTAGGAAAGGGAAATAATGAGAAGTCACTGAACCTGGTAGACCCAAATTCTTTGCAGGATGATTCTCAGCTTGAAATTGTTCCAGATCCAGCATCTGCAGAGTCAAGACAACTTGACACTTCTTTATTTGATACCAATGAGGAGCTTGCTTTGACGCCTATGGGTATGATGACAGCTGGTCAAATCATTGACGAAAGAAGTTATGCATCTGGCCTTGTACCCCAAGGGATAGTTGAAGAAAAGAAAGTTCTTCCTCTGGAAAATCCCAAGGAGATGATGGCTCGTTGGAAGGCTAACAATTTGGATTTAAAAACTGTGGTTAAGGATGCCTTGCTCTCTGGTCGACTCCCTTTAGCTGTTCTTCAATTGCATCTTCAACATTCTAAAGACGTGGTTGAAGATGGAGAGCATCATGATACATTTACTGAAGTTCGTGATATCGGAAGAGCTATTGCTTATGACCTATTTTTGAAGGGTGAACCTGGGGTTGCCATTGCCACTCTGCAAAGGCTCGGAGAGGATGTAGAAGCATGTCTCAATCAGTTGGTATTTGGCACAGTGAGAAGATCTCTTCGATATCAAATTGCTGAAGAAATGAGAAAACTTGGTTTCTTAAGACCATATGAAGACAATGTACTGGAGAGGATATCTCTTATCGAGAGGCTTTATCCTAGCAGCCACTTTTGGGAAACATATCTTGCTCGTCGAAAGGAACTCCTTAAAGCTGCACTACCTTTTGACTCCAGTGAAATTAGCTTGCACCTTGGGGGGTCGTCTTTGTTTCAGCATCTCAAGATTGAGTGTGGTGAGGTTGATGGTGTTGTTCTAGGTTCTTGGACTAAAATCAATGAGAGTGCATCTGAGCATGCACCTGATGAAACCGATGCAGTTGCTGGCTACTGGGCTGCAGCAGCTGTGTGGTCAAATGCTTGGGATCAGAGAACATTTGATCATATAGTCTTGGATCAGCCCTTAGTCATGGGTGTTCATGTTCCATGGGATTCTCAGCTGGAGTATTATATGTGTCACAATGACTGGGATGAAGTTCTTAAGCTGTTGGATCTCATTCCTGAGGATGTTTTATATGATGGAAGTTTGCAAATTGCTTTGGATGGTCCGAAACAGTCTTCTGGCGTAAATTATTCAGTTTCTTCCCGTAGCGAGTATATATGCTCCATCGAAGAAGTGGATGCTGTACTTATGGATGTTCCTTATATCAAGATATTTAGATTGCCTGGAGATATCCGGTGCTCCTTGTGGCTAACAACACTCATGGAGCAGGAACTAGCAAGAAAACTTATATTTTTAAAAGAATATTGGGAAAACGCTCTAGATGTAGTATATCTTCTGGCCCGGGCAGGTGTCATCCTTGGTAATTGTGAAGTTTCATTTAAGGAGGAAACTTGTACGCCATCGTTAGATCTTTGTTTGTCCATAAAGAAGGGAGGAGCAAATGTTGATACCCTGAATGCTGTACACAAGCTATTCATACATTACTGCACACAGTATAATCTGCCAAACCTTCTGGATCTGTACCTTGATCATCACGAATTGGTCCTAGATAATGATTCACTCAGTTCATTGCAGGAAGCTGTGGGTGATTCTCACTGGGCAAAATGGTTGCTACTCTCCAGGATCAAGGGCCGGGAGTATGATGCTTCATTTTCAAACGCACGCTCAATTATGTCACGTAACGGGGCACCAAATAGTGAACCCAGTGTTCCAGAGATTGATGAAATGGTTTGTACTGTTGATGACATTGCCGATGGGGCAGGAGAAATGGCAGCTTTAGCAACTATGATGTGTGCTCCAGTGCCAATTCAAAAATCCTTGAGTACTGGCAGTGTGAACAGGCACACTAACTCTTCTGCGCAGTGCACTTTGGAGAATTTGAGATCATTTCTTCAGAGATTCCCTACCTTATGGAGTAAGCTTGTTAGTGCTTGTCTTGGGGAAGATATATCCGGGAACCTTTTGCGGACCAAGACTAAGAATGAGTATTTGAATTGGCGTGACGGTGTATTCTTCTCAACGGCACGTGATACCTCACTCCTCCAGATGTTGCCTTGTTGGTTTCCTAAGGCGGTTCGTAGATTAGTTCAGCTTTATATTCAGGGACCTCTTGGATGGCTGTCTTTCTCAGGATATCCTACAGGGGAATATCTACTGCATAGAGGTGTTGAGTTCTTTATAAATGTCGACGATCCGACAGAGATAAGTGCCATATCTTGGGAAGCAATCATCCAGAAGCACATCGAAGAGGAACTTCATCACACAAAAACTGAGGGAACCGAGCTTGGACTTGAGCACTTCCTGCATCGTGGACGGCCACTCGCAGCTTTTAATGCTTTTCTGGAACATAGAGTTGAAAAGTTAAAACTGGAAGATCAATCTGGCTCTTCGATACATGGACAAAGAAATATGCAGTCAGATGTTCCGATGCTACTTGCACCTCTGACGCAGAGTGATGAGTCACTTCTTTCATCTGTCATACCACTTGCTATCACACACTTTGGGGATTCTGTGTTGGTGGCTTCGTGTGCCTTCCTTCTTGAGCTTTGTGGTTTATCTGCTAGCATGCTTCGGATAGACGTTGCATCCTTGAGAAGAATATCATCCTTCTATAAATCAAATGGTAATGCGGATATGGCACATCAGAAATCGCTGAAGCGCTCAATGTTTCATAGTGTATCTAGTGAAGATGATCTAATGGGGTCACTAGCTAGGGCCCTTGCTAACGAATACGCTTATCCTGATATTTCAAGTGTCCCTAAGCAAAAACAGAATCCAAGTATTTCTGGTAGTCAACCCGGTCTTCCGCTAATGCTTGTTCTTCATCATTTGGAACAAGCTAGTCTTCCAGAGATTGGAGTAGGTAGAAAAACAAGTGGATACTGGCTGTTAACAGGTGATGGTGATGGGTCTGAACTGCGGTCTCAACAGACATCTGCTAGCTTGCATTGGAGTTTAGTTACTCTTTTTTGTCAGATGCATAAGATTCCTCTGAGCACCAAATATCTTGCCATGCTAGCAAGAGACAATGATTGGGTTGGATTTTTATCTGAGGCGCAGCTTGGAGGATACCCATTTGATACAGTGCTCAATGTGGCATCGAAGGAGTTTGGTGATCAACGTTTAAAAGCTCACATACTGACTGTTTTGAGATATGCCAACTCAAAAAAGAAAGCCACCACATCGTTCTCAGATGACCCAAGCAGAGGACTTTCATGTTCCCCCTCAGAGGGTGGAGCTTATGTCTCTGCTGAACTCTTTCGTGTTTTGGCATACTCTGAGAAGCTAAAGAATCCTGGGGAATACCTTTTGTCAAAAGCTAAGGAATTCTCGTGGTCTATTTTGGCTCTCATAGCATCATGCTTTCCGGATGTTTCTCCTTTATCATGCCTAACAATTTGGTTGGAAATTACCGCAGCCAGGGAAACGTCGTCTATCAAGGTGAACGATATAACGACTAAAATAGCTGAAAATATAGGAGCAGCTGTTGTATCTACAAATTCCTTGCCGACGGATGCTAGAGGTGTCCAGTTTCATTACAATAGGAGGAATCCCAAACGGAGACGACTTACTGCACATACATCTGTGGATTTATTGGCTTCAGCCAATAGTTTAAACATCTCTGCTGGTAAAACATTTTGTTCTCATAGAACAGAAGCTGCAGAAGATGAAAAAGCGGAAGATAGCAGTGTTATTGATGATTCGTCTGATGAGCATGCGTCTCTATCAAAAATGGTCGCAGTGCTTTGTGAACAACGCTTGTTTCTTCCTCTGCTAAAAGCTTTTGACTTGTTCCTCCCGTCCTGCTCTCTACTGCCATTTTTCCGTGCTCTTCAGGCATTTTCTCAAATGCGCCTGTCTGAAGCTTCAGCACATCTGGGTTCTTTTTGGGGTAGAGTTAAAGAGGAATCAATGCATTTTCAGTCAAATACAGCCAAAGATGTCAATTTTGGGGCATCTTGGATCAGCAGAACAGCTGTTAAGGCTGCTGATGCTGTTTTGTCAGCTTGTCCATCTCCATATGAGAAAAGATGCTTACTACAACTTCTTGCTGCAACTGATTTTGGTGATGGGGGATCTGCGGCAACATATTATCGGCGTCTTTATTGGAAAGTTAACTTGGCTGAGCCATCACTCCGGGAGAATGATCTTGACTTAGGAAATGAGTCCCTTGATGATGGCTCTCTTCTGACAGCTTTAGAAAAGAACAGGCAATGGGAGCAAGCAAGAAACTGGGCCAAGCAACTTGAAACCATTGGTGCCACTTGGACATCCTCTGTACATCATGTTACTGAAACACAGGCAGAATCCATGGTTGCAGAATGGAAAGAATTCCTTTGGGATGTTCCAGAAGAGAGAATCGCATTGTGGGGTCACTGCCAAACTCTGTTCATCAGATACTCTTTCCCTGCTTTACAGGCAGGTTTATTTTTCCTTAGGCATGCAGAAGTGGTGGAGAAAGATCTTCCTGCAAGAGAGATCTATGAGCTGTTACTGCTATCCCTTCAGTGGCTAAGTGGGCTAACAACTCTGTCGCACCCGGTTTATCCTTTGCATCTTCTCCGAGAAATCGAGACTAGGGTATGGTTACTGGCTGTAGAAGCAGAATCACATGTGAAAAATGTCGGAGCATTTAGTCCGAGTAGTATCGGAAAAGACATGGTGAATGGATATAGTTCAAATCTTATAGATCGAACTGCAAGTATCATAACAAAAATGGACAGCCATATTTCTTCAGCAACGAAAAATAGAATTGGAGAAAAACATGACGCCAGGGCCGCTGGCCAAGGAAATCAAAGGAACCAAGACACGAGCACTTCAATATTTGGTGCTAGCACTAAGCCCAAGAGGAGAGCAAAAGGAAATGTCCCACAAATAAGACATTTTGTGGATTCTTCAGATAGGAATACTGACTTTGAAGATTCTTCATCTCTTATAAACATCAAAAGCGAGTTCCAGCTTCAAGAGGAGAGCACGGGTTTGGAAATATCCTTATCGAAGTGGGAAGAAAGTATAGAACCTGCAGAGTTGGAAAGAGCTGTTCTGTCCTTACTGGAGTTCGGACAAGTAACAGCTGCTAAGCAGCTCCAACTTAAGCTTGCTCCAGGGAATCTACCTTCTGAGCTGATAATTTTGGATGCTGTTATGAAGCTAGCAATGCTTTCTACGCCTTGCAGACAAGTACTATTGTCAATGTTAGATGATGAAGTTCGCTCTGTCATTCAATCACACAGTCTAAAAATAGACCAACCTATGATTGAACCGCTGCAGATTTTGGAGAATCTATCAACCATTTTAAATGAAGGCAGTGGACGAGGGCTGGCAAGAAAAATCATCGCAGTTATAAAAGCTGCTAACATACTGGGCCTTACATTCACTGAGGCATATCAAAAGCAGCCGATAGAGTTGTTACGATTACTTTCTCTTAAAGCACAAGATTCTTTTGAGGAAGCATGTCTCCTTGTCCAAACGCATTCAATGCCCGCTGCTAGTATCGCTCAAATACTTGCAGAATCCTTTCTAAAGGGTTTATTGGCTGCTCATCGTGGAGGATATATAGATTCTCAAAAGGAGGAAGGGCCTGCTCCACTTTTGTGGAGATTTTCAGACTTTTTGAAGTGGGCGGAGCTTTGTCCATCTGAACAAGAAATTGGTCATGCATTAATGCGTCTTGTAATAACCGGGCAGGAAATACCACACGCGTGTGAGGTTGAGCTTCTTATTCTGTCTCATCACTTCTACAAGTCATCCACGTGCCTTGATGGAGTAGATGTTCTTGTGGCACTTGCTGCCACAAGGGTTGAGGCTTATGTTGCTGAGGGAGATTTCTCATGCCTGGCTCGTCTAATAACTGGAGTTGGAAACTTTCATGCTCTTAATTTCATTCTCAATATTCTAATCGAAAACGGGCAGCTTGATCTTTTACTACAAAAATTTTCTGCTGCTGCTGATGCAAACACTGGCACCGCTCAGGCTGTCAGAAGTTTCCGGATGGCTGTTCTGACCTCGTTGAATCTGTATAACCCAAATGACCATGATGCATTCGCAATGGTATATAAACACTTTGACATGAAGCATGAAACGGCTACTTTGCTAGAGGCACGTGCAGATCAGGCTGCACAGCAGTGGTTTCTACGGTATGACAAGGATCAAAATGAAGATCTCCTTGATTCTATGCGCTATTACATTGAAGCTGCCGAAGTTCACACTTCTATTGATGCTGGAAACAAAGCGCGAAAGGCTTGTGGTCAGGCTTCCCTTGTCTCCCTTCAGATAAGGATGCCAGATTCCAAGTGGCTCTGTTTATCTGAAACAAACGCAAGAAGAGCACTTGTAGATCAATCCCGTTTCCAGGAGGCTCTGATTGTAGCAGAAGCTTATGGTCTTAACCAGCCAAGCGAATGGGCGCTGGTGCTTTGGAATCTGATGCTCAAACCTGAACTAGCGGAAGATTTTGTGGCTGAATTTGTAGCGGTGCTTCCCCTACAGGCTTCAATGCTTCTGGAACTGGCTAGATTCTACAGAGCGGAAATGGCAGCTAGGGGAGACCAGTCTCAGTTCTCAGTCTGGCTCACAGGAGGAGGCTTACCAGCTGAGTGGGCCAAATATATGTGGAGGTCATTCAGGTGTTTGTTGAAAAGAACACGGGATTTGAGGTTGCGGTTACAGCTTGCTACAACAGCAACGGGATTTGCTGACATGGTAGATGTGTGTATGAATGCGTTGGATAAGGTCCCGGAAAATGCTGGACCACTTGTACTAAAGAAAGGACATGGAGGAGGATACTTACCACTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spatacsin_C

Protein Analysis

2513

Amino Acids

280.33

Weight (kDa)

5.51

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spatacsin_C PF14649 2799 - 3090 1.2e-71 Spatacsin C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2921, 5678, 6086
AarI CACCTGC 1 cut(s) 2078
Acc16I TGCGCA 1 cut(s) 2601
Acc36I ACCTGC 3 cut(s) 2078, 5153, 5770
AccB1I GGYRCC 3 cut(s) 2428, 5000, 6704
AccB7I CCANNNNNTGG 2 cut(s) 2958, 3596
AccBSI CCGCTC 2 cut(s) 6710, 7268
AccI GTMKAC 4 cut(s) 325, 768, 1072, 2928
AccII CGCG 4 cut(s) 90, 454, 6443, 6976
AccIII TCCGGA 2 cut(s) 4108, 6729
AclI AACGTT 1 cut(s) 3862
AcoI YGGCCR 2 cut(s) 3056, 5524
AcsI RAATTY 9 cut(s) 83, 646, 775, 2617, 4067, 4240, 5072, 6674, 7205
AcvI CACGTG 3 cut(s) 2768, 6495, 6845
AcyI GRCGYC 3 cut(s) 884, 4840, 5511
AfeI AGCGCT 1 cut(s) 3401
AflII CTTAAG 3 cut(s) 1342, 1778, 5834
AflIII ACRYGT 3 cut(s) 573, 5353, 6441
AhdI GACNNNNNGTC 1 cut(s) 906
AjiI CACGTC 1 cut(s) 1120
AjuI GAANNNNNNNTTGG 4 cut(s) 4844, 4876, 6375, 6407
AleI CACNNNNGTG 5 cut(s) 6334, 6442, 6444, 6865, 7537
AloI GAACNNNNNNTCC 2 cut(s) 6498, 6530
Alw44I GTGCAC 1 cut(s) 2605
AlwNI CAGNNNCTG 4 cut(s) 824, 1622, 1643, 4400
Ama87I CYCGRG 1 cut(s) 2082
Aor13HI TCCGGA 2 cut(s) 4108, 6729
Aor51HI AGCGCT 1 cut(s) 3401
ApaI GGGCCC 1 cut(s) 3460
ApaLI GTGCAC 1 cut(s) 2605
ApoI RAATTY 9 cut(s) 83, 646, 775, 2617, 4067, 4240, 5072, 6674, 7205
AseI ATTAAT 1 cut(s) 6404
Asp700I GAANNNNTTC 4 cut(s) 807, 3999, 5072, 6258
AspA2I CCTAGG 1 cut(s) 590
AspLEI GCGC 8 cut(s) 456, 2602, 3402, 3799, 4596, 6921, 6976, 7150
AsuC2I CCSGG 9 cut(s) 2083, 2084, 2375, 2695, 3540, 4880, 5282, 6424, 7478
AsuNHI GCTAGC 5 cut(s) 3302, 3680, 3757, 5573, 5893
AvaI CYCGRG 1 cut(s) 2082
AvaII GGWCC 8 cut(s) 177, 301, 1844, 2284, 2709, 2846, 7474, 7490
AvrII CCTAGG 1 cut(s) 590
AxyI CCTNAGG 3 cut(s) 1800, 2808, 5177
BaeGI GKGCMC 3 cut(s) 2431, 2609, 3460
BalI TGGCCA 1 cut(s) 5526
BanI GGYRCC 3 cut(s) 2428, 5000, 6704
BanII GRGCYC 1 cut(s) 3460
BarI GAAGNNNNNNTAC 6 cut(s) 1347, 1379, 2058, 2090, 7203, 7235
BauI CACGAG 2 cut(s) 4072, 5551
BbrPI CACGTG 3 cut(s) 2768, 6495, 6845
BbsI GAAGAC 4 cut(s) 1362, 1848, 3536, 3578
BbvCI CCTCAGC 2 cut(s) 419, 6558
BceAI ACGGC 3 cut(s) 2778, 3071, 6840
BcgI CGANNNNNNTGC 2 cut(s) 1601, 1635
BciVI GTATCC 3 cut(s) 3614, 3803, 7517
BclI TGATCA 3 cut(s) 1678, 2269, 3856
BcnI CCSGG 9 cut(s) 2083, 2084, 2375, 2695, 3540, 4880, 5282, 6424, 7478
BfmI CTRYAG 9 cut(s) 825, 1635, 2880, 4401, 5337, 5763, 6008, 7226, 7261
BfoI RGCGCY 2 cut(s) 3403, 7151
BfrI CTTAAG 3 cut(s) 1342, 1778, 5834
BfuAI ACCTGC 3 cut(s) 2078, 5153, 5770
BfuI GTATCC 3 cut(s) 3614, 3803, 7517
BglI GCCNNNNNGGC 1 cut(s) 2498
BglII AGATCT 5 cut(s) 1295, 2149, 5203, 5220, 6901
BlnI CCTAGG 1 cut(s) 590
BlpI GCTNAGC 2 cut(s) 4866, 5820
BmcAI AGTACT 2 cut(s) 2566, 5925
Bme18I GGWCC 8 cut(s) 177, 301, 1844, 2284, 2709, 2846, 7474, 7490
BmeRI GACNNNNNGTC 1 cut(s) 906
BmeT110I CYCGRG 1 cut(s) 2082
BmgBI CACGTC 1 cut(s) 1120
BmrI ACTGGG 6 cut(s) 1639, 1774, 2347, 2439, 4984, 6113
BmtI GCTAGC 5 cut(s) 3306, 3684, 3761, 5577, 5897
BmuI ACTGGG 6 cut(s) 1639, 1774, 2347, 2439, 4984, 6113
BpiI GAAGAC 4 cut(s) 1362, 1848, 3536, 3578
BplI GAGNNNNNCTC 4 cut(s) 1282, 1314, 1363, 1395
Bpu10I CCTNAGC 5 cut(s) 419, 3501, 4062, 6558, 6711
Bpu1102I GCTNAGC 2 cut(s) 4866, 5820
BpuEI CTTGAG 6 cut(s) 56, 1505, 2581, 3053, 3302, 3352
BpuMI CCSGG 9 cut(s) 2083, 2084, 2375, 2695, 3540, 4880, 5282, 6424, 7478
Bsa29I ATCGAT 1 cut(s) 633
BsaAI YACGTR 4 cut(s) 2420, 2768, 6495, 6845
BsaBI GATNNNNATC 1 cut(s) 3787
BsaHI GRCGYC 3 cut(s) 884, 4840, 5511
BsaI GGTCTC 2 cut(s) 3669, 7281
BsaWI WCCGGW 3 cut(s) 1974, 4108, 6729
Bse21I CCTNAGG 3 cut(s) 1800, 2808, 5177
Bse3DI GCAATG 6 cut(s) 1221, 2309, 2487, 4961, 5904, 6795
Bse8I GATNNNNATC 1 cut(s) 3787
BseAI TCCGGA 2 cut(s) 4108, 6729
BseCI ATCGAT 1 cut(s) 633
BseJI GATNNNNATC 1 cut(s) 3787
BseMI GCAATG 6 cut(s) 1221, 2309, 2487, 4961, 5904, 6795
BseRI GAGGAG 7 cut(s) 884, 2190, 2771, 5605, 5723, 7335, 7533
BseSI GKGCMC 3 cut(s) 2431, 2609, 3460
BsgI GTGCAG 6 cut(s) 26, 1440, 2212, 4299, 6843, 6867
Bsh1236I CGCG 4 cut(s) 90, 454, 6443, 6976
BshNI GGYRCC 3 cut(s) 2428, 5000, 6704
BshVI ATCGAT 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 2082
BslFI GGGAC 6 cut(s) 314, 2859, 3482, 4894, 5594, 7460
BsmBI CGTCTC 2 cut(s) 4297, 4467
BsmFI GGGAC 6 cut(s) 314, 2859, 3482, 4894, 5594, 7460
BsmI GAATGC 4 cut(s) 2203, 6214, 6782, 7465
Bso31I GGTCTC 2 cut(s) 3669, 7281
BsoBI CYCGRG 1 cut(s) 2082
Bsp120I GGGCCC 1 cut(s) 3456
Bsp13I TCCGGA 2 cut(s) 4108, 6729
Bsp1407I TGTACA 2 cut(s) 2203, 5020
Bsp1720I GCTNAGC 2 cut(s) 4866, 5820
Bsp19I CCATGG 2 cut(s) 1724, 5052
Bsp68I TCGCGA 1 cut(s) 90
BspDI ATCGAT 1 cut(s) 633
BspEI TCCGGA 2 cut(s) 4108, 6729
BspFNI CGCG 4 cut(s) 90, 454, 6443, 6976
BspHI TCATGA 1 cut(s) 1141
BspMAI CTGCAG 5 cut(s) 829, 1639, 4405, 5767, 6012
BspMI ACCTGC 3 cut(s) 2078, 5153, 5770
BspOI GCTAGC 5 cut(s) 3306, 3684, 3761, 5577, 5897
BspQI GCTCTTC 4 cut(s) 1168, 3133, 4578, 7060
BspT107I GGYRCC 3 cut(s) 2428, 5000, 6704
BspTI CTTAAG 3 cut(s) 1342, 1778, 5834
BspTNI GGTCTC 2 cut(s) 3669, 7281
BsrBI CCGCTC 2 cut(s) 6710, 7268
BsrDI GCAATG 6 cut(s) 1221, 2309, 2487, 4961, 5904, 6795
BsrGI TGTACA 2 cut(s) 2203, 5020
BssNI GRCGYC 3 cut(s) 884, 4840, 5511
BssSI CACGAG 2 cut(s) 4072, 5551
BssT1I CCWWGG 9 cut(s) 499, 590, 952, 996, 1492, 1724, 2098, 5052, 5527
Bst2BI CACGAG 2 cut(s) 4072, 5551
BstACI GRCGYC 3 cut(s) 884, 4840, 5511
BstAFI CTTAAG 3 cut(s) 1342, 1778, 5834
BstAPI GCANNNNNTGC 3 cut(s) 3043, 6220, 6530
BstAUI TGTACA 2 cut(s) 2203, 5020
BstBAI YACGTR 4 cut(s) 2420, 2768, 6495, 6845
BstDSI CCRYGG 2 cut(s) 1724, 5052
BstFNI CGCG 4 cut(s) 90, 454, 6443, 6976
BstH2I RGCGCY 2 cut(s) 3403, 7151
BstHHI GCGC 8 cut(s) 456, 2602, 3402, 3799, 4596, 6921, 6976, 7150
BstNSI RCATGY 9 cut(s) 418, 577, 1263, 1598, 3310, 4460, 5186, 5357, 6198
BstSFI CTRYAG 9 cut(s) 825, 1635, 2880, 4401, 5337, 5763, 6008, 7226, 7261
BstSLI GKGCMC 3 cut(s) 2431, 2609, 3460
BstUI CGCG 4 cut(s) 90, 454, 6443, 6976
BstV2I GAAGAC 4 cut(s) 1362, 1848, 3536, 3578
BstXI CCANNNNNNTGG 3 cut(s) 6336, 7143, 7338
Bsu15I ATCGAT 1 cut(s) 633
Bsu36I CCTNAGG 3 cut(s) 1800, 2808, 5177
BsuI GTATCC 3 cut(s) 3614, 3803, 7517
BsuTUI ATCGAT 1 cut(s) 633
BtgI CCRYGG 2 cut(s) 1724, 5052
BtgZI GCGATG 1 cut(s) 6061
BtrI CACGTC 1 cut(s) 1120
BtsI GCAGTG 8 cut(s) 193, 2578, 2609, 4435, 4488, 5116, 6055, 6872
BtuMI TCGCGA 1 cut(s) 90
BveI ACCTGC 3 cut(s) 2078, 5153, 5770
CaiI CAGNNNCTG 4 cut(s) 824, 1622, 1643, 4400
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 8 cut(s) 456, 2602, 3402, 3799, 4596, 6921, 6976, 7150
Cfr9I CCCGGG 1 cut(s) 2082
ClaI ATCGAT 1 cut(s) 633
CseI GACGC 7 cut(s) 311, 892, 3196, 4449, 4829, 5519, 6398
CsiI ACCWGGT 1 cut(s) 762
CspCI CAANNNNNGTGG 4 cut(s) 6481, 6516, 6523, 6558
DraI TTTAAA 5 cut(s) 1038, 2037, 3867, 4356, 6039
DriI GACNNNNNGTC 1 cut(s) 906
EaeI YGGCCR 2 cut(s) 3056, 5524
Eam1105I GACNNNNNGTC 1 cut(s) 906
EciI GGCGGA 1 cut(s) 6380
Eco130I CCWWGG 9 cut(s) 499, 590, 952, 996, 1492, 1724, 2098, 5052, 5527
Eco24I GRGCYC 1 cut(s) 3460
Eco31I GGTCTC 2 cut(s) 3669, 7281
Eco32I GATATC 5 cut(s) 1168, 1307, 1377, 1972, 2876
Eco47I GGWCC 8 cut(s) 177, 301, 1844, 2284, 2709, 2846, 7474, 7490
Eco47III AGCGCT 1 cut(s) 3401
Eco72I CACGTG 3 cut(s) 2768, 6495, 6845
Eco81I CCTNAGG 3 cut(s) 1800, 2808, 5177
Eco88I CYCGRG 1 cut(s) 2082
EcoO109I RGGNCCY 6 cut(s) 301, 2846, 3456, 3457, 6320, 7474
EcoRI GAATTC 2 cut(s) 4067, 5072
EcoRV GATATC 5 cut(s) 1168, 1307, 1377, 1972, 2876
EcoT14I CCWWGG 9 cut(s) 499, 590, 952, 996, 1492, 1724, 2098, 5052, 5527
EcoT22I ATGCAT 5 cut(s) 937, 3723, 4659, 6403, 6784
EcoT38I GRGCYC 1 cut(s) 3460
ErhI CCWWGG 9 cut(s) 499, 590, 952, 996, 1492, 1724, 2098, 5052, 5527
Esp3I CGTCTC 2 cut(s) 4297, 4467
FaqI GGGAC 6 cut(s) 314, 2859, 3482, 4894, 5594, 7460
FauI CCCGC 1 cut(s) 6232
FauNDI CATATG 2 cut(s) 1351, 4762
FbaI TGATCA 3 cut(s) 1678, 2269, 3856
FblI GTMKAC 4 cut(s) 325, 768, 1072, 2928
FriOI GRGCYC 1 cut(s) 3460
FspI TGCGCA 1 cut(s) 2601
GlaI GCGC 8 cut(s) 455, 2601, 3401, 3798, 4595, 6920, 6975, 7149
HaeII RGCGCY 2 cut(s) 3403, 7151
HgaI GACGC 7 cut(s) 311, 892, 3196, 4449, 4829, 5519, 6398
HhaI GCGC 8 cut(s) 456, 2602, 3402, 3799, 4596, 6921, 6976, 7150
Hin1I GRCGYC 3 cut(s) 884, 4840, 5511
Hin6I GCGC 8 cut(s) 454, 2600, 3400, 3797, 4594, 6919, 6974, 7148
HinP1I GCGC 8 cut(s) 454, 2600, 3400, 3797, 4594, 6919, 6974, 7148
HincII GTYRAC 6 cut(s) 326, 1073, 2929, 3536, 3633, 4858
HindII GTYRAC 6 cut(s) 326, 1073, 2929, 3536, 3633, 4858
HindIII AAGCTT 6 cut(s) 147, 2660, 4521, 4605, 5837, 7116
HpaI GTTAAC 2 cut(s) 3633, 4858
Hpy99I CGWCG 6 cut(s) 112, 327, 1437, 2933, 4174, 4255
HpyCH4IV ACGT 8 cut(s) 1119, 2419, 2767, 3321, 3862, 4169, 6494, 6844
HpySE526I ACGT 8 cut(s) 1119, 2419, 2767, 3321, 3862, 4169, 6494, 6844
Hsp92I GRCGYC 3 cut(s) 884, 4840, 5511
HspAI GCGC 8 cut(s) 454, 2600, 3400, 3797, 4594, 6919, 6974, 7148
Kpn2I TCCGGA 2 cut(s) 4108, 6729
Ksp22I TGATCA 3 cut(s) 1678, 2269, 3856
KspAI GTTAAC 2 cut(s) 3633, 4858
LguI GCTCTTC 4 cut(s) 1168, 3133, 4578, 7060
MabI ACCWGGT 1 cut(s) 762
MaeII ACGT 8 cut(s) 1119, 2419, 2767, 3321, 3862, 4169, 6494, 6844
MbiI CCGCTC 2 cut(s) 6710, 7268
MfeI CAATTG 1 cut(s) 1094
MlsI TGGCCA 1 cut(s) 5526
MluI ACGCGT 1 cut(s) 6441
MluNI TGGCCA 1 cut(s) 5526
MlyI GAGTC 6 cut(s) 380, 839, 1068, 1463, 3209, 4916
Mox20I TGGCCA 1 cut(s) 5526
Mph1103I ATGCAT 5 cut(s) 937, 3723, 4659, 6403, 6784
MroI TCCGGA 2 cut(s) 4108, 6729
MroXI GAANNNNTTC 4 cut(s) 807, 3999, 5072, 6258
MscI TGGCCA 1 cut(s) 5526
MslI CAYNNNNRTG 8 cut(s) 2904, 6334, 6442, 6444, 6493, 6865, 7366, 7537
Msp20I TGGCCA 1 cut(s) 5526
MspCI CTTAAG 3 cut(s) 1342, 1778, 5834
MssI GTTTAAAC 1 cut(s) 4356
MunI CAATTG 1 cut(s) 1094
Mva1269I GAATGC 4 cut(s) 2203, 6214, 6782, 7465
MvnI CGCG 4 cut(s) 90, 454, 6443, 6976
NciI CCSGG 9 cut(s) 2083, 2084, 2375, 2695, 3540, 4880, 5282, 6424, 7478
NcoI CCATGG 2 cut(s) 1724, 5052
NdeI CATATG 2 cut(s) 1351, 4762
NheI GCTAGC 5 cut(s) 3302, 3680, 3757, 5573, 5893
NmuCI GTSAC 7 cut(s) 753, 1754, 2415, 2741, 3201, 3444, 5114
NruI TCGCGA 1 cut(s) 90
NsbI TGCGCA 1 cut(s) 2601
NsiI ATGCAT 5 cut(s) 937, 3723, 4659, 6403, 6784
NspI RCATGY 9 cut(s) 418, 577, 1263, 1598, 3310, 4460, 5186, 5357, 6198
OliI CACNNNNGTG 5 cut(s) 6334, 6442, 6444, 6865, 7537
PaeI GCATGC 5 cut(s) 418, 1598, 3310, 4460, 5186
PagI TCATGA 1 cut(s) 1141
PaqCI CACCTGC 1 cut(s) 2078
PciI ACATGT 2 cut(s) 573, 5353
PciSI GCTCTTC 4 cut(s) 1168, 3133, 4578, 7060
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PctI GAATGC 4 cut(s) 2203, 6214, 6782, 7465
PdmI GAANNNNTTC 4 cut(s) 807, 3999, 5072, 6258
PflMI CCANNNNNTGG 2 cut(s) 2958, 3596
PfoI TCCNGGA 5 cut(s) 2360, 2693, 4878, 7092, 7476
PleI GAGTC 6 cut(s) 379, 838, 1068, 1463, 3208, 4915
PmaCI CACGTG 3 cut(s) 2768, 6495, 6845
PmeI GTTTAAAC 1 cut(s) 4356
PmlI CACGTG 3 cut(s) 2768, 6495, 6845
PpsI GAGTC 6 cut(s) 379, 838, 1068, 1463, 3208, 4915
Ppu21I YACGTR 4 cut(s) 2420, 2768, 6495, 6845
PpuMI RGGWCCY 3 cut(s) 301, 2846, 7474
PscI ACATGT 2 cut(s) 573, 5353
PshBI ATTAAT 1 cut(s) 6404
PsiI TTATAA 3 cut(s) 2921, 5678, 6086
Psp1406I AACGTT 1 cut(s) 3862
Psp5II RGGWCCY 3 cut(s) 301, 2846, 7474
PspCI CACGTG 3 cut(s) 2768, 6495, 6845
PspOMI GGGCCC 1 cut(s) 3456
PspPPI RGGWCCY 3 cut(s) 301, 2846, 7474
PstI CTGCAG 5 cut(s) 829, 1639, 4405, 5767, 6012
PstNI CAGNNNCTG 4 cut(s) 824, 1622, 1643, 4400
PvuII CAGCTG 7 cut(s) 905, 1643, 1738, 4226, 4718, 5816, 7334
RruI TCGCGA 1 cut(s) 90
RseI CAYNNNNRTG 8 cut(s) 2904, 6334, 6442, 6444, 6493, 6865, 7366, 7537
SalI GTCGAC 3 cut(s) 324, 1071, 2927
SapI GCTCTTC 4 cut(s) 1168, 3133, 4578, 7060
ScaI AGTACT 2 cut(s) 2566, 5925
SchI GAGTC 6 cut(s) 380, 839, 1068, 1463, 3209, 4916
SexAI ACCWGGT 1 cut(s) 762
SfcI CTRYAG 9 cut(s) 825, 1635, 2880, 4401, 5337, 5763, 6008, 7226, 7261
SinI GGWCC 8 cut(s) 177, 301, 1844, 2284, 2709, 2846, 7474, 7490
SmaI CCCGGG 1 cut(s) 2084
SmiMI CAYNNNNRTG 8 cut(s) 2904, 6334, 6442, 6444, 6493, 6865, 7366, 7537
SmlI CTYRAG 9 cut(s) 71, 1342, 1520, 1778, 2560, 3032, 3281, 3331, 5834
SmoI CTYRAG 9 cut(s) 71, 1342, 1520, 1778, 2560, 3032, 3281, 3331, 5834
SphI GCATGC 5 cut(s) 418, 1598, 3310, 4460, 5186
SrfI GCCCGGGC 1 cut(s) 2084
SspI AATATT 4 cut(s) 697, 2045, 5565, 6634
StyI CCWWGG 9 cut(s) 499, 590, 952, 996, 1492, 1724, 2098, 5052, 5527
TaiI ACGT 8 cut(s) 1122, 2422, 2770, 3324, 3865, 4172, 6497, 6847
TatI WGTACW 8 cut(s) 1363, 1924, 2203, 2474, 2564, 5020, 5923, 7498
TauI GCSGC 2 cut(s) 4826, 5522
TseFI GTSAC 7 cut(s) 753, 1754, 2415, 2741, 3201, 3444, 5114
Tsp45I GTSAC 7 cut(s) 753, 1754, 2415, 2741, 3201, 3444, 5114
TspGWI ACGGA 4 cut(s) 96, 4268, 4315, 4556
TspMI CCCGGG 1 cut(s) 2082
Van91I CCANNNNNTGG 2 cut(s) 2958, 3596
Vha464I CTTAAG 3 cut(s) 1342, 1778, 5834
VneI GTGCAC 1 cut(s) 2605
VpaK11BI GGWCC 8 cut(s) 177, 301, 1844, 2284, 2709, 2846, 7474, 7490
VspI ATTAAT 1 cut(s) 6404
XapI RAATTY 9 cut(s) 83, 646, 775, 2617, 4067, 4240, 5072, 6674, 7205
XbaI TCTAGA 1 cut(s) 2059
XceI RCATGY 9 cut(s) 418, 577, 1263, 1598, 3310, 4460, 5186, 5357, 6198
XcmI CCANNNNNNNNNTGG 3 cut(s) 1003, 1912, 7032
XmaI CCCGGG 1 cut(s) 2082
XmaJI CCTAGG 1 cut(s) 590
XmiI GTMKAC 4 cut(s) 325, 768, 1072, 2928
XmnI GAANNNNTTC 4 cut(s) 807, 3999, 5072, 6258
ZrmI AGTACT 2 cut(s) 2566, 5925
Zsp2I ATGCAT 5 cut(s) 937, 3723, 4659, 6403, 6784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.