AT5G01880

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003563
Physical Location & Seq
Forward (+)
338896 .. 340253
1358 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01880.1

Sequence Viewer

Length: 480 bp
ATGCATCGACTGCTACTGGAATCCCACGGCGGCGGGAACGAGACGAGCGGAAGCGGCGGGGGAGATGGGTACACGAGGGACATGAACTTCGACGCAAACATGGTGATCATACTTGCGGCTCTTCTCTGCGCGCTGATACTAGCGCTTGGGCTGAATTCGATTCTCCGGTGCGCGATGCGATGCGGTTTCGGGTTATCATCCTCTGCGGCGGCGGGGACGGTGGCCGACAGAGCTGGATTAAAGAAGAGAGAACTGAAGAAGTTTCCAGTAGCTGAGTACGGGTCGGGGGAAGTGAAGATAGCGGCGACGGAGTGCGCAATCTGTCTGGGGGAATTCGCGGACGGCGAGAGAGTGCGAGTACTGCCGCCGTGTAATCACAGTTTCCACATGTCATGCATCGACACGTGGCTTGTCTCTCACTCTTCATGTCCCAATTGTCGGCATTCACTCATCGAGGTCCACGTGGCGGGGTCTGAGTGA

Protein Analysis

159

Amino Acids

16.79

Weight (kDa)

6.37

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 104 - 147 1.4e-15 Ring finger domain
zf-RING_11 PF17123 104 - 133 2.1e-12 RING-like zinc finger
zf-C3HC4_2 PF13923 104 - 146 4.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 105 - 146 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 105 - 144 1.6e-06 RING-type zinc-finger
zf-rbx1 PF12678 117 - 147 4.4e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 316
AccBSI CCGCTC 1 cut(s) 48
AccII CGCG 3 cut(s) 131, 173, 338
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 2 cut(s) 154, 332
AcuI CTGAAG 1 cut(s) 275
AcvI CACGTG 2 cut(s) 405, 463
AfaI GTAC 3 cut(s) 71, 278, 360
AfeI AGCGCT 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 438, 466
AflIII ACRYGT 2 cut(s) 387, 402
AluBI AGCT 2 cut(s) 233, 272
AluI AGCT 2 cut(s) 233, 272
Alw26I GTCTC 2 cut(s) 35, 418
AlwNI CAGNNNCTG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 144
AoxI GGCC 1 cut(s) 222
ApoI RAATTY 2 cut(s) 154, 332
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AspLEI GCGC 5 cut(s) 131, 133, 145, 173, 317
AspS9I GGNCC 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 1 cut(s) 457
BauI CACGAG 1 cut(s) 73
BbrPI CACGTG 2 cut(s) 405, 463
BccI CCATC 1 cut(s) 59
BceAI ACGGC 3 cut(s) 43, 352, 358
BclI TGATCA 1 cut(s) 105
BcoDI GTCTC 2 cut(s) 35, 418
BfaI CTAG 1 cut(s) 140
BfoI RGCGCY 1 cut(s) 146
BisI GCNGC 7 cut(s) 31, 55, 117, 207, 210, 303, 365
BlsI GCNGC 7 cut(s) 32, 56, 118, 208, 211, 304, 366
BmcAI AGTACT 1 cut(s) 360
Bme18I GGWCC 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 457
BmsI GCATC 4 cut(s) 13, 165, 170, 405
BsaAI YACGTR 2 cut(s) 405, 463
BsaJI CCNNGG 1 cut(s) 25
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 466
Bse1I ACTGG 2 cut(s) 21, 266
BseDI CCNNGG 1 cut(s) 25
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 2 cut(s) 438, 466
BseMII CTCAG 2 cut(s) 264, 465
BseNI ACTGG 2 cut(s) 21, 266
BsePI GCGCGC 1 cut(s) 129
Bsh1236I CGCG 3 cut(s) 131, 173, 338
BshFI GGCC 1 cut(s) 224
BsiSI CCGG 1 cut(s) 166
BslFI GGGAC 3 cut(s) 92, 229, 414
BslI CCNNNNNNNGG 2 cut(s) 438, 466
BsmAI GTCTC 2 cut(s) 35, 418
BsmBI CGTCTC 1 cut(s) 35
BsmFI GGGAC 3 cut(s) 92, 229, 414
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 1 cut(s) 224
Bsp143I GATC 1 cut(s) 105
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 2 cut(s) 265, 466
BspFNI CGCG 3 cut(s) 131, 173, 338
BspQI GCTCTTC 1 cut(s) 126
BsrBI CCGCTC 1 cut(s) 48
BsrI ACTGG 2 cut(s) 21, 266
BssECI CCNNGG 1 cut(s) 25
BssHII GCGCGC 1 cut(s) 129
BssMI GATC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 73
Bst4CI ACNGT 2 cut(s) 220, 380
Bst6I CTCTTC 3 cut(s) 126, 239, 427
BstAPI GCANNNNNTGC 1 cut(s) 10
BstBAI YACGTR 2 cut(s) 405, 463
BstC8I GCNNGC 1 cut(s) 131
BstDEI CTNAG 2 cut(s) 273, 474
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 3 cut(s) 131, 173, 338
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 5 cut(s) 131, 133, 145, 173, 317
BstKTI GATC 1 cut(s) 108
BstMAI GTCTC 2 cut(s) 35, 418
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 4 cut(s) 10, 54, 230, 361
BstNSI RCATGY 1 cut(s) 391
BstUI CGCG 3 cut(s) 131, 173, 338
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 2 cut(s) 188, 193
BtsCI GGATG 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 131
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 5 cut(s) 131, 133, 145, 173, 317
Cfr13I GGNCC 1 cut(s) 457
CseI GACGC 1 cut(s) 101
Csp6I GTAC 3 cut(s) 70, 277, 359
CviAII CATG 5 cut(s) 82, 100, 388, 393, 426
CviJI RGCY 6 cut(s) 119, 151, 224, 233, 272, 409
CviKI_1 RGCY 6 cut(s) 119, 151, 224, 233, 272, 409
CviQI GTAC 3 cut(s) 70, 277, 359
DdeI CTNAG 2 cut(s) 273, 474
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 3 cut(s) 126, 239, 427
EarI CTCTTC 3 cut(s) 126, 239, 427
Eco47I GGWCC 1 cut(s) 457
Eco47III AGCGCT 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 275
Eco72I CACGTG 2 cut(s) 405, 463
EcoRI GAATTC 2 cut(s) 154, 332
EcoT22I ATGCAT 2 cut(s) 6, 398
Esp3I CGTCTC 1 cut(s) 35
FaeI CATG 5 cut(s) 85, 103, 391, 396, 429
FaiI YATR 6 cut(s) 83, 101, 110, 389, 394, 427
FaqI GGGAC 3 cut(s) 92, 229, 414
FatI CATG 5 cut(s) 81, 99, 387, 392, 425
FauI CCCGC 4 cut(s) 26, 50, 205, 460
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 7 cut(s) 31, 55, 117, 207, 210, 303, 365
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 7 cut(s) 31, 55, 117, 207, 210, 303, 365
FspBI CTAG 1 cut(s) 140
FspI TGCGCA 1 cut(s) 316
GlaI GCGC 5 cut(s) 130, 132, 144, 172, 316
GluI GCNGC 7 cut(s) 31, 55, 117, 207, 210, 303, 365
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 1 cut(s) 224
HapII CCGG 1 cut(s) 166
HgaI GACGC 1 cut(s) 101
HhaI GCGC 5 cut(s) 131, 133, 145, 173, 317
Hin1II CATG 5 cut(s) 85, 103, 391, 396, 429
Hin6I GCGC 5 cut(s) 129, 131, 143, 171, 315
HinP1I GCGC 5 cut(s) 129, 131, 143, 171, 315
HinfI GANTC 2 cut(s) 20, 160
HpaII CCGG 1 cut(s) 166
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 72, 460
Hpy188I TCNGA 1 cut(s) 475
Hpy8I GTNNAC 2 cut(s) 72, 460
Hpy99I CGWCG 2 cut(s) 95, 310
HpyCH4III ACNGT 2 cut(s) 220, 380
HpyCH4IV ACGT 2 cut(s) 404, 462
HpyCH4V TGCA 2 cut(s) 4, 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 54, 230, 361
HpyF3I CTNAG 2 cut(s) 273, 474
HpySE526I ACGT 2 cut(s) 404, 462
Hsp92II CATG 5 cut(s) 85, 103, 391, 396, 429
HspAI GCGC 5 cut(s) 129, 131, 143, 171, 315
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 1 cut(s) 105
LguI GCTCTTC 1 cut(s) 126
LpnPI CCDG 5 cut(s) 2, 179, 219, 279, 311
LweI GCATC 4 cut(s) 13, 165, 170, 405
MaeI CTAG 1 cut(s) 140
MaeII ACGT 2 cut(s) 404, 462
MalI GATC 1 cut(s) 107
MbiI CCGCTC 1 cut(s) 48
MboI GATC 1 cut(s) 105
MboII GAAGA 5 cut(s) 113, 256, 268, 307, 414
MfeI CAATTG 1 cut(s) 433
MluCI AATT 3 cut(s) 154, 332, 433
MnlI CCTC 3 cut(s) 69, 211, 448
Mph1103I ATGCAT 2 cut(s) 6, 398
MseI TTAA 1 cut(s) 239
MspI CCGG 1 cut(s) 166
MunI CAATTG 1 cut(s) 433
Mva1269I GAATGC 1 cut(s) 442
MvnI CGCG 3 cut(s) 131, 173, 338
MwoI GCNNNNNNNGC 4 cut(s) 10, 54, 230, 361
NdeII GATC 1 cut(s) 105
NlaIII CATG 5 cut(s) 85, 103, 391, 396, 429
NsbI TGCGCA 1 cut(s) 316
NsiI ATGCAT 2 cut(s) 6, 398
NspI RCATGY 1 cut(s) 391
PauI GCGCGC 1 cut(s) 129
PciI ACATGT 1 cut(s) 387
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 459
PctI GAATGC 1 cut(s) 442
PfeI GAWTC 2 cut(s) 20, 160
PkrI GCNGC 7 cut(s) 32, 56, 118, 208, 211, 304, 366
PmaCI CACGTG 2 cut(s) 405, 463
PmlI CACGTG 2 cut(s) 405, 463
Ppu21I YACGTR 2 cut(s) 405, 463
PscI ACATGT 1 cut(s) 387
PspCI CACGTG 2 cut(s) 405, 463
PspPI GGNCC 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 272
PteI GCGCGC 1 cut(s) 129
RsaI GTAC 3 cut(s) 71, 278, 360
RsaNI GTAC 3 cut(s) 70, 277, 359
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 7 cut(s) 31, 55, 117, 207, 210, 303, 365
Sau3AI GATC 1 cut(s) 105
Sau96I GGNCC 1 cut(s) 457
ScaI AGTACT 1 cut(s) 360
SetI ASST 5 cut(s) 235, 274, 407, 459, 465
SfaNI GCATC 4 cut(s) 13, 165, 170, 405
SinI GGWCC 1 cut(s) 457
Sse9I AATT 3 cut(s) 154, 332, 433
SspMI CTAG 1 cut(s) 140
TaaI ACNGT 2 cut(s) 220, 380
TaiI ACGT 2 cut(s) 407, 465
TaqI TCGA 5 cut(s) 7, 90, 158, 399, 453
TasI AATT 3 cut(s) 154, 332, 433
TatI WGTACW 1 cut(s) 358
TauI GCSGC 7 cut(s) 33, 57, 119, 209, 212, 305, 367
TfiI GAWTC 2 cut(s) 20, 160
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 98, 414
TspGWI ACGGA 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 457
XapI RAATTY 2 cut(s) 154, 332
XceI RCATGY 1 cut(s) 391
XspI CTAG 1 cut(s) 140
ZrmI AGTACT 1 cut(s) 360
Zsp2I ATGCAT 2 cut(s) 6, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.