MD04G1174300.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003563
Physical Location & Seq
Forward (+)
26579665 .. 26580282
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1174300.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCATCGACATCTCCTTGGAACAGAGGTCAACACGGCGGCGCCGTTGTCCAATGGAAACAGAGCCGCCGATTCGTACGTTAGCGAGGCCAAGTTCGACACCAACATGGTGATCATATTGGCTGCCTTATTGTGTGCTTTGATATGCGCACTTGGTCTCAATTCGTTCGTCCGCTGCGCCCTCCGCTGCAGCCACAGGTTCGCTCTCGAGACGCCGGAGCAGGCGGCTGCGCAGTTGGCCGCCACCGGCTTGAAGAAGAGGCATTTAAGGCAAATCCCAATTGCGGTGTACGGCTCCGGCGTGAATATTCCGGCCACCGAATGCCCCATCTGTCTTGCGGAGTTCGAAGACGGAGAAAAAGTTCGAGTCTTGCCGAAATGCCATCACGGATTCCACGTGAGGTGCATAGACACGTGGCTTTTGTCGCACTCGTCGTGCCCGAATTGTCGGCATTCGCTGCTGAGTACTAGTTCTGCTGCCCCGGCCGCCGCTGCTGTTGCGGCTGCAGATTCAACTCAAGAAGCTAATGCGGCGGCGGAGGGGTCATCGGCAGCCGCCACCAACGAGTCCAGCCAGCAAGGTGGCAGAGTAGTCGTAGTGGTTGAGCAGGGGACTTAA

Protein Analysis

206

Amino Acids

21.49

Weight (kDa)

6.74

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 107 - 150 4.4e-15 Ring finger domain
zf-RING_11 PF17123 107 - 136 3.5e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 107 - 149 6e-09 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 107 - 150 4.9e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 108 - 149 1.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 148, 231
AccB1I GGYRCC 1 cut(s) 40
AcoI YGGCCR 3 cut(s) 237, 312, 483
AcvI CACGTG 2 cut(s) 397, 414
AcyI GRCGYC 2 cut(s) 41, 212
AfaI GTAC 3 cut(s) 77, 290, 466
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 2 cut(s) 253, 513
AhlI ACTAGT 1 cut(s) 467
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 1 cut(s) 524
AluI AGCT 1 cut(s) 524
Alw26I GTCTC 2 cut(s) 161, 203
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 4 cut(s) 87, 237, 312, 483
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 4 cut(s) 43, 149, 179, 232
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 121
AsuII TTCGAA 1 cut(s) 345
AvaI CYCGRG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 440
BanI GGYRCC 1 cut(s) 40
BbrPI CACGTG 2 cut(s) 397, 414
BbsI GAAGAC 1 cut(s) 354
BccI CCATC 2 cut(s) 335, 390
BceAI ACGGC 3 cut(s) 28, 51, 307
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BclI TGATCA 1 cut(s) 111
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 2 cut(s) 161, 203
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 2 cut(s) 187, 504
BfoI RGCGCY 1 cut(s) 44
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 482
BmeT110I CYCGRG 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 42, 295
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 482
BsaAI YACGTR 2 cut(s) 397, 414
BsaHI GRCGYC 2 cut(s) 41, 212
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 16, 480
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 16, 480
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 440
BseX3I CGGCCG 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 4 cut(s) 89, 239, 314, 485
BshNI GGYRCC 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 486
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 5 cut(s) 215, 246, 297, 311, 482
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 2 cut(s) 161, 203
BsmBI CGTCTC 1 cut(s) 203
BsmI GAATGC 2 cut(s) 326, 451
BsnI GGCC 4 cut(s) 89, 239, 314, 485
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 1 cut(s) 111
BspANI GGCC 4 cut(s) 89, 239, 314, 485
BspCNI CTCAG 1 cut(s) 453
BspLI GGNNCC 2 cut(s) 42, 295
BspMAI CTGCAG 2 cut(s) 191, 508
BspT104I TTCGAA 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 245
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 16, 480
BssMI GATC 1 cut(s) 111
BssNI GRCGYC 2 cut(s) 41, 212
BssT1I CCWWGG 1 cut(s) 16
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 2 cut(s) 41, 212
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBAI YACGTR 2 cut(s) 397, 414
BstBI TTCGAA 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 222, 575
BstDEI CTNAG 1 cut(s) 461
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 4 cut(s) 43, 149, 179, 232
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 2 cut(s) 161, 203
BstMBI GATC 1 cut(s) 111
BstMCI CGRYCG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 2 cut(s) 187, 504
BstSLI GKGCMC 1 cut(s) 440
BstV2I GAAGAC 1 cut(s) 354
BstXI CCANNNNNNTGG 1 cut(s) 581
BstZI CGGCCG 1 cut(s) 483
BsuRI GGCC 4 cut(s) 89, 239, 314, 485
Cac8I GCNNGC 2 cut(s) 222, 575
CfoI GCGC 4 cut(s) 43, 149, 179, 232
Cfr10I RCCGGY 1 cut(s) 245
CseI GACGC 1 cut(s) 220
Csp6I GTAC 3 cut(s) 76, 289, 465
CviAII CATG 1 cut(s) 106
CviQI GTAC 3 cut(s) 76, 289, 465
DdeI CTNAG 1 cut(s) 461
DinI GGCGCC 1 cut(s) 42
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
EaeI YGGCCR 3 cut(s) 237, 312, 483
EagI CGGCCG 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 551
EclXI CGGCCG 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 483
Eco72I CACGTG 2 cut(s) 397, 414
Eco88I CYCGRG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 16
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 16
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 1 cut(s) 109
FaiI YATR 4 cut(s) 107, 116, 145, 407
FatI CATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 111
FspAI RTGCGCAY 1 cut(s) 148
FspBI CTAG 1 cut(s) 468
FspI TGCGCA 2 cut(s) 148, 231
GlaI GCGC 4 cut(s) 42, 148, 178, 231
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 4 cut(s) 89, 239, 314, 485
HapII CCGG 5 cut(s) 215, 246, 297, 311, 482
HgaI GACGC 1 cut(s) 220
HhaI GCGC 4 cut(s) 43, 149, 179, 232
Hin1I GRCGYC 2 cut(s) 41, 212
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 4 cut(s) 41, 147, 177, 230
HinP1I GCGC 4 cut(s) 41, 147, 177, 230
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 5 cut(s) 71, 366, 390, 509, 566
HpaII CCGG 5 cut(s) 215, 246, 297, 311, 482
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 31, 289
Hpy188III TCNNGA 3 cut(s) 206, 208, 518
Hpy8I GTNNAC 2 cut(s) 31, 289
Hpy99I CGWCG 1 cut(s) 436
HpyCH4IV ACGT 3 cut(s) 78, 396, 413
HpyCH4V TGCA 4 cut(s) 4, 189, 405, 506
HpyF3I CTNAG 1 cut(s) 461
HpySE526I ACGT 3 cut(s) 78, 396, 413
Hsp92I GRCGYC 2 cut(s) 41, 212
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 4 cut(s) 41, 147, 177, 230
KasI GGCGCC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 217, 299
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 468
MaeII ACGT 3 cut(s) 78, 396, 413
MalI GATC 1 cut(s) 113
MboI GATC 1 cut(s) 111
MboII GAAGA 3 cut(s) 265, 268, 359
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 3 cut(s) 160, 279, 442
Mly113I GGCGCC 1 cut(s) 41
MlyI GAGTC 2 cut(s) 375, 575
MnlI CCTC 6 cut(s) 19, 79, 191, 252, 393, 532
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 2 cut(s) 266, 616
MslI CAYNNNNRTG 1 cut(s) 104
MspA1I CMGCKG 3 cut(s) 174, 186, 491
MspI CCGG 5 cut(s) 215, 246, 297, 311, 482
MspR9I CCNGG 1 cut(s) 482
MunI CAATTG 1 cut(s) 279
Mva1269I GAATGC 2 cut(s) 326, 451
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 1 cut(s) 482
NdeII GATC 1 cut(s) 111
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 2 cut(s) 42, 295
NsbI TGCGCA 2 cut(s) 148, 231
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 206
PcsI WCGNNNNNNNCGW 5 cut(s) 41, 297, 393, 431, 437
PctI GAATGC 2 cut(s) 326, 451
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 71, 390, 509
Pfl23II CGTACG 1 cut(s) 75
PleI GAGTC 2 cut(s) 374, 574
PluTI GGCGCC 1 cut(s) 44
PmaCI CACGTG 2 cut(s) 397, 414
PmlI CACGTG 2 cut(s) 397, 414
PpsI GAGTC 2 cut(s) 374, 574
Ppu21I YACGTR 2 cut(s) 397, 414
PspCI CACGTG 2 cut(s) 397, 414
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 42, 295
PstI CTGCAG 2 cut(s) 191, 508
RsaI GTAC 3 cut(s) 77, 290, 466
RsaNI GTAC 3 cut(s) 76, 289, 465
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 2 cut(s) 266, 616
Sau3AI GATC 1 cut(s) 111
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 2 cut(s) 375, 575
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 1 cut(s) 440
SetI ASST 8 cut(s) 30, 81, 200, 399, 404, 416, 526, 583
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 2 cut(s) 187, 504
SfoI GGCGCC 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 345
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 206, 516
SmoI CTYRAG 2 cut(s) 206, 516
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 3 cut(s) 160, 279, 442
SspDI GGCGCC 1 cut(s) 40
SspI AATATT 1 cut(s) 307
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 16
TaiI ACGT 3 cut(s) 81, 399, 416
TaqI TCGA 5 cut(s) 7, 96, 207, 345, 364
TasI AATT 3 cut(s) 160, 279, 442
TatI WGTACW 1 cut(s) 464
TfiI GAWTC 3 cut(s) 71, 390, 509
Tru1I TTAA 2 cut(s) 266, 616
Tru9I TTAA 2 cut(s) 266, 616
TspGWI ACGGA 2 cut(s) 366, 402
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 466
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.