Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003563

Physical Location & Seq

Genomic Coordinates Forward (+)

27184858 .. 27186311

Gene Structure 1454 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1167200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGGTAACTCTTCATCACCACCGTCCACACCGCCTACTTCTAGACCAACCAACAACACCACCAGCATCACCAACCCATGGGAGCAGACCACGTGGTACAAACTCCGATGAAGCCAATGGGAGCAAGGCACGTGATACATACGCCAATGATGCCAATTTTGACACCAACATGGTCATCATCCTAGCAGCCTTGCTGTGCGCTCTGATATTTGCATTGGGACTAAACTCAATCGTCCGGTGTGCGCTGAGATGCAGCAGAAGGTTCTCTTCTGAGACACCAGACCAGACGGCAGCGCGGTTGGCGGCCACGGGGCTTAGAAAGCGCACATTGCGCCAGATTCCCGTCGTGGTATATGGGACTGGACAAAATATTCCCTCCACAGATTGTCCAATTTGCCTTGGAGAGTTTACAGATGGGCAGAAAGTGAGGCTGCTGCCGAAATGCAGCCATGGATTTCATGTCAGGTGCATAGACACATGGTTTTTGTCACACTCGTCGTGCCCAATTTGCCGCCATTCGTTGCTTGAGCAGCCTGCTGCAGGCAGTTTGGATTCGGCCGAAAATGTTGATGGTGGTGGTGCTAGACATGCTGGAAATGTATCATCAGGTGGACAAGGTGATGCGACAATCTCTGTTGATGAGGCTGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009555 GO:0009791 GO:0009889 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010498 GO:0010565 GO:0010817 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022414 GO:0030163 GO:0031323 GO:0031326 GO:0032350 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0036211 GO:0042304 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0046885 GO:0046890 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048609 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051603 GO:0061458 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080090 GO:0080140 GO:0080141 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

217

Amino Acids

23.05

Weight (kDa)

8.13

Isoelectric Point (pI)

34.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	128 - 171	6e-15	Ring finger domain
zf-RING_11	PF17123	128 - 157	6e-13	RING-like zinc finger
zf-C3HC4_2	PF13923	129 - 170	2.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	129 - 171	3.5e-06	RING-H2 zinc finger domain
zf-C3HC4	PF00097	129 - 170	9.2e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10910 AT5G01880
fragaria_vesca	FvH4_6g09940 FvH4_7g25880 FvH4_7g25880
malus_domestica	MD01G1167200.v1.1 MD04G1174300.v1.1 MD07G1233300.v1.1 MD12G1187900.v1.1
prunus_persica	Prupe.2G263800_v2.0.a1 Prupe.6G293100_v2.0.a1
pyrus_communis	pycom01g18030 pycom04g15370
rosa_chinensis	RchiOBHm_Chr1g0372171 RchiOBHm_Chr3g0460531
rosa_laevigata	RLG00000024996 RLG00000026917
rosa_multiflora	Rmu_co8033776.1_g000001 Rmu_sc0001755.1_g000021 Rmu_sc0004267.1_g000002 Rmu_sc0006046.1_g000005
rosa_roxburghii	Rroxscaffold_4G00285170 Rroxscaffold_6G00419400
rosa_rugosa	Rorug01G0370100 Rorug03G0043300
rosa_samantha	Rh1AG378600 Rh1BG341900 Rh1CG355500 Rh1DG373000 Rh3AG101500 Rh3BG105500 Rh3CG107300 Rh3DG106900
rosa_wichuraiana	Rw1G033310 Rw3G008640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	77
AccII	CGCG	1 cut(s)	295
AciI	CCGC	4 cut(s)	31, 295, 302, 511
AcoI	YGGCCR	2 cut(s)	303, 555
AcvI	CACGTG	2 cut(s)	92, 131
AfaI	GTAC	1 cut(s)	97
AfiI	CCNNNNNNNGG	2 cut(s)	77, 539
Alw26I	GTCTC	1 cut(s)	266
AoxI	GGCC	2 cut(s)	303, 555
ApeKI	GCWGC	8 cut(s)	185, 252, 290, 430, 433, 444, 529, 536
AspLEI	GCGC	5 cut(s)	200, 244, 295, 324, 333
AsuHPI	GGTGA	3 cut(s)	8, 60, 629
BaeGI	GKGCMC	1 cut(s)	503
BbrPI	CACGTG	2 cut(s)	92, 131
BbvI	GCAGC	8 cut(s)	197, 264, 302, 417, 420, 456, 523, 541
BccI	CCATC	2 cut(s)	407, 563
BceAI	ACGGC	1 cut(s)	303
BcoDI	GTCTC	1 cut(s)	266
BfaI	CTAG	4 cut(s)	41, 182, 582, 649
BfmI	CTRYAG	1 cut(s)	537
BmsI	GCATC	4 cut(s)	74, 139, 239, 610
BpuEI	CTTGAG	1 cut(s)	545
BsaAI	YACGTR	2 cut(s)	92, 131
BsaJI	CCNNGG	4 cut(s)	76, 306, 397, 448
BsaWI	WCCGGW	1 cut(s)	234
Bsc4I	CCNNNNNNNGG	2 cut(s)	77, 539
Bse1I	ACTGG	1 cut(s)	364
Bse3DI	GCAATG	1 cut(s)	326
BseDI	CCNNGG	4 cut(s)	76, 306, 397, 448
BseGI	GGATG	1 cut(s)	177
BseLI	CCNNNNNNNGG	2 cut(s)	77, 539
BseMI	GCAATG	1 cut(s)	326
BseMII	CTCAG	2 cut(s)	236, 261
BseNI	ACTGG	1 cut(s)	364
BseSI	GKGCMC	1 cut(s)	503
BseX3I	CGGCCG	1 cut(s)	555
BseXI	GCAGC	8 cut(s)	197, 264, 302, 417, 420, 456, 523, 541
Bsh1236I	CGCG	1 cut(s)	295
Bsh1285I	CGRYCG	1 cut(s)	558
BshFI	GGCC	2 cut(s)	305, 557
BsiEI	CGRYCG	1 cut(s)	558
BsiSI	CCGG	1 cut(s)	235
BslFI	GGGAC	2 cut(s)	231, 370
BslI	CCNNNNNNNGG	2 cut(s)	77, 539
BsmAI	GTCTC	1 cut(s)	266
BsmFI	GGGAC	2 cut(s)	231, 370
BsnI	GGCC	2 cut(s)	305, 557
Bsp1286I	GDGCHC	1 cut(s)	503
Bsp19I	CCATGG	2 cut(s)	76, 448
BspACI	CCGC	4 cut(s)	31, 295, 302, 511
BspANI	GGCC	2 cut(s)	305, 557
BspCNI	CTCAG	2 cut(s)	237, 262
BspFNI	CGCG	1 cut(s)	295
BspMAI	CTGCAG	1 cut(s)	541
BsrDI	GCAATG	1 cut(s)	326
BsrI	ACTGG	1 cut(s)	364
BssECI	CCNNGG	4 cut(s)	76, 306, 397, 448
BssT1I	CCWWGG	3 cut(s)	76, 397, 448
Bst4CI	ACNGT	1 cut(s)	23
Bst6I	CTCTTC	2 cut(s)	15, 271
BstBAI	YACGTR	2 cut(s)	92, 131
BstC8I	GCNNGC	3 cut(s)	534, 541, 646
BstDEI	CTNAG	3 cut(s)	245, 270, 314
BstDSI	CCRYGG	3 cut(s)	76, 306, 448
BstENI	CCTNNNNNAGG	1 cut(s)	537
BstF5I	GGATG	1 cut(s)	177
BstFNI	CGCG	1 cut(s)	295
BstHHI	GCGC	5 cut(s)	200, 244, 295, 324, 333
BstMAI	GTCTC	1 cut(s)	266
BstMCI	CGRYCG	1 cut(s)	558
BstMWI	GCNNNNNNNGC	8 cut(s)	149, 299, 319, 328, 330, 507, 529, 587
BstNSI	RCATGY	1 cut(s)	590
BstSFI	CTRYAG	1 cut(s)	537
BstSLI	GKGCMC	1 cut(s)	503
BstUI	CGCG	1 cut(s)	295
BstV1I	GCAGC	8 cut(s)	197, 264, 302, 417, 420, 456, 523, 541
BstZI	CGGCCG	1 cut(s)	555
BsuRI	GGCC	2 cut(s)	305, 557
BtgI	CCRYGG	3 cut(s)	76, 306, 448
BtsCI	GGATG	1 cut(s)	177
Cac8I	GCNNGC	3 cut(s)	534, 541, 646
CfoI	GCGC	5 cut(s)	200, 244, 295, 324, 333
Csp6I	GTAC	1 cut(s)	96
CviAII	CATG	6 cut(s)	77, 169, 449, 458, 477, 587
CviQI	GTAC	1 cut(s)	96
DdeI	CTNAG	3 cut(s)	245, 270, 314
EaeI	YGGCCR	2 cut(s)	303, 555
EagI	CGGCCG	1 cut(s)	555
Eam1104I	CTCTTC	2 cut(s)	15, 271
EarI	CTCTTC	2 cut(s)	15, 271
EclXI	CGGCCG	1 cut(s)	555
Eco130I	CCWWGG	3 cut(s)	76, 397, 448
Eco52I	CGGCCG	1 cut(s)	555
Eco72I	CACGTG	2 cut(s)	92, 131
EcoNI	CCTNNNNNAGG	1 cut(s)	537
EcoT14I	CCWWGG	3 cut(s)	76, 397, 448
ErhI	CCWWGG	3 cut(s)	76, 397, 448
FaeI	CATG	6 cut(s)	80, 172, 452, 461, 480, 590
FalI	AAGNNNNNCTT	2 cut(s)	250, 282
FaqI	GGGAC	2 cut(s)	231, 370
FatI	CATG	6 cut(s)	76, 168, 448, 457, 476, 586
FokI	GGATG	1 cut(s)	164
FspBI	CTAG	4 cut(s)	41, 182, 582, 649
GlaI	GCGC	5 cut(s)	199, 243, 294, 323, 332
HaeIII	GGCC	2 cut(s)	305, 557
HapII	CCGG	1 cut(s)	235
HhaI	GCGC	5 cut(s)	200, 244, 295, 324, 333
Hin1II	CATG	6 cut(s)	80, 172, 452, 461, 480, 590
Hin6I	GCGC	5 cut(s)	198, 242, 293, 322, 331
HinP1I	GCGC	5 cut(s)	198, 242, 293, 322, 331
HinfI	GANTC	2 cut(s)	337, 551
HpaII	CCGG	1 cut(s)	235
HphI	GGTGA	3 cut(s)	8, 60, 629
Hpy166II	GTNNAC	3 cut(s)	26, 408, 611
Hpy188I	TCNGA	3 cut(s)	106, 204, 271
Hpy188III	TCNNGA	1 cut(s)	41
Hpy8I	GTNNAC	3 cut(s)	26, 408, 611
Hpy99I	CGWCG	2 cut(s)	347, 499
HpyAV	CCTTC	1 cut(s)	252
HpyCH4III	ACNGT	1 cut(s)	23
HpyCH4IV	ACGT	2 cut(s)	91, 130
HpyCH4V	TGCA	5 cut(s)	212, 252, 444, 468, 539
HpyF10VI	GCNNNNNNNGC	8 cut(s)	149, 299, 319, 328, 330, 507, 529, 587
HpyF3I	CTNAG	3 cut(s)	245, 270, 314
HpySE526I	ACGT	2 cut(s)	91, 130
Hsp92II	CATG	6 cut(s)	80, 172, 452, 461, 480, 590
HspAI	GCGC	5 cut(s)	198, 242, 293, 322, 331
LmnI	GCTCC	2 cut(s)	81, 120
Lsp1109I	GCAGC	8 cut(s)	197, 264, 302, 417, 420, 456, 523, 541
LweI	GCATC	4 cut(s)	74, 139, 239, 610
MaeI	CTAG	4 cut(s)	41, 182, 582, 649
MaeII	ACGT	2 cut(s)	91, 130
MaeIII	GTNAC	2 cut(s)	4, 486
MboII	GAAGA	1 cut(s)	258
MhlI	GDGCHC	1 cut(s)	503
MluCI	AATT	3 cut(s)	154, 390, 504
MnlI	CCTC	3 cut(s)	385, 420, 634
MslI	CAYNNNNRTG	1 cut(s)	167
MspI	CCGG	1 cut(s)	235
MvnI	CGCG	1 cut(s)	295
MwoI	GCNNNNNNNGC	8 cut(s)	149, 299, 319, 328, 330, 507, 529, 587
NcoI	CCATGG	2 cut(s)	76, 448
NlaIII	CATG	6 cut(s)	80, 172, 452, 461, 480, 590
NmuCI	GTSAC	1 cut(s)	486
NspI	RCATGY	1 cut(s)	590
PfeI	GAWTC	2 cut(s)	337, 551
PflMI	CCANNNNNTGG	1 cut(s)	77
PmaCI	CACGTG	2 cut(s)	92, 131
PmlI	CACGTG	2 cut(s)	92, 131
Ppu21I	YACGTR	2 cut(s)	92, 131
PspCI	CACGTG	2 cut(s)	92, 131
PstI	CTGCAG	1 cut(s)	541
RsaI	GTAC	1 cut(s)	97
RsaNI	GTAC	1 cut(s)	96
RseI	CAYNNNNRTG	1 cut(s)	167
SduI	GDGCHC	1 cut(s)	503
SetI	ASST	6 cut(s)	94, 133, 263, 467, 610, 619
SfaNI	GCATC	4 cut(s)	74, 139, 239, 610
SfcI	CTRYAG	1 cut(s)	537
SmiMI	CAYNNNNRTG	1 cut(s)	167
SmlI	CTYRAG	1 cut(s)	524
SmoI	CTYRAG	1 cut(s)	524
Sse9I	AATT	3 cut(s)	154, 390, 504
SsiI	CCGC	4 cut(s)	31, 295, 302, 511
SspI	AATATT	1 cut(s)	370
SspMI	CTAG	4 cut(s)	41, 182, 582, 649
StyI	CCWWGG	3 cut(s)	76, 397, 448
TaaI	ACNGT	1 cut(s)	23
TaiI	ACGT	2 cut(s)	94, 133
TasI	AATT	3 cut(s)	154, 390, 504
TauI	GCSGC	2 cut(s)	305, 513
TfiI	GAWTC	2 cut(s)	337, 551
TseFI	GTSAC	1 cut(s)	486
TseI	GCWGC	8 cut(s)	185, 252, 290, 430, 433, 444, 529, 536
Tsp45I	GTSAC	1 cut(s)	486
TspDTI	ATGAA	2 cut(s)	123, 446
Van91I	CCANNNNNTGG	1 cut(s)	77
XagI	CCTNNNNNAGG	1 cut(s)	537
XbaI	TCTAGA	1 cut(s)	40
XceI	RCATGY	1 cut(s)	590
XspI	CTAG	4 cut(s)	41, 182, 582, 649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.