Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003563

Physical Location & Seq

Genomic Coordinates Forward (+)

26994722 .. 26995324

Gene Structure 603 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1187900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 603 bp

ATGCATCGACATCTCCTCGGAACAGCGCTGAACACGGCGGCGCCGTCGTCCAATGGGAACAGAACCGCCGATTCGTTCATTAACGAGGCCAACTTCGACACCAACATGGTGATCATATTGGCGGCCTTGCTGTGTGCTCTGATATGCGCGCTTGGTCTCAACTCGATCGTCCGCTGCGCCCTCCGCTGCAGCCATAGGTTCGTTCTCGAGACGCCGGAGCAGGCGGCAGCGCGGTTGGCCGCCACCGGCTTGAAGAAGAGCCATTTGAGGCAAATCCCAATTGCGGTGTACGGCTCCGGCGTGAATATTCCGGCCACCGAATGCCCAATCTGTCTTACGGAGTTCGAAGACGGAGAAAAAGTTCGAGTCTTGCCAAAATGCAATCACGGATTCCATGTGAGGTGCATAGACACGTGGTTTCTGTCGCACTCCTCGTGTCCGAATTGTCGGCACTCGCTGCTGAGTACGAGTTCAACGGCAGCTGCCGTTGCTGCAGATTCAAGTGGAGAAGCTGCGGCGGCGGAGGAGGAGTCACCGGCAGCGGCCAATGAGTCCAGCCAGCAAGGTGGCAGAGTAGTGGTAGTGGTCGAGCAGGGGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

201

Amino Acids

21.07

Weight (kDa)

6.22

Isoelectric Point (pI)

44.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	107 - 150	2.5e-15	Ring finger domain
zf-RING_11	PF17123	107 - 136	2.2e-12	RING-like zinc finger
zf-C3HC4_2	PF13923	107 - 149	2.3e-08	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	107 - 150	1.2e-06	RING-H2 zinc finger domain
zf-C3HC4	PF00097	108 - 149	2.6e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10910 AT5G01880
fragaria_vesca	FvH4_6g09940 FvH4_7g25880 FvH4_7g25880
malus_domestica	MD01G1167200.v1.1 MD04G1174300.v1.1 MD07G1233300.v1.1 MD12G1187900.v1.1
prunus_persica	Prupe.2G263800_v2.0.a1 Prupe.6G293100_v2.0.a1
pyrus_communis	pycom01g18030 pycom04g15370
rosa_chinensis	RchiOBHm_Chr1g0372171 RchiOBHm_Chr3g0460531
rosa_laevigata	RLG00000024996 RLG00000026917
rosa_multiflora	Rmu_co8033776.1_g000001 Rmu_sc0001755.1_g000021 Rmu_sc0004267.1_g000002 Rmu_sc0006046.1_g000005
rosa_roxburghii	Rroxscaffold_4G00285170 Rroxscaffold_6G00419400
rosa_rugosa	Rorug01G0370100 Rorug03G0043300
rosa_samantha	Rh1AG378600 Rh1BG341900 Rh1CG355500 Rh1DG373000 Rh3AG101500 Rh3BG105500 Rh3CG107300 Rh3DG106900
rosa_wichuraiana	Rw1G033310 Rw3G008640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	40
AccII	CGCG	2 cut(s)	149, 232
AcoI	YGGCCR	3 cut(s)	237, 312, 543
AcvI	CACGTG	1 cut(s)	414
AcyI	GRCGYC	2 cut(s)	41, 212
AfaI	GTAC	2 cut(s)	290, 466
AfeI	AGCGCT	1 cut(s)	27
AfiI	CCNNNNNNNGG	1 cut(s)	283
AflIII	ACRYGT	1 cut(s)	411
AgsI	TTSAA	3 cut(s)	253, 474, 501
AloI	GAACNNNNNNTCC	2 cut(s)	345, 377
AluBI	AGCT	2 cut(s)	482, 512
AluI	AGCT	2 cut(s)	482, 512
Alw21I	GWGCWC	1 cut(s)	139
Alw26I	GTCTC	2 cut(s)	161, 203
Ama87I	CYCGRG	1 cut(s)	206
Aor51HI	AGCGCT	1 cut(s)	27
AoxI	GGCC	5 cut(s)	87, 123, 237, 312, 543
Asp700I	GAANNNNTTC	1 cut(s)	360
AspLEI	GCGC	6 cut(s)	28, 43, 149, 151, 179, 232
AsuHPI	GGTGA	2 cut(s)	121, 525
AsuII	TTCGAA	1 cut(s)	345
AvaI	CYCGRG	1 cut(s)	206
BanI	GGYRCC	1 cut(s)	40
BauI	CACGAG	1 cut(s)	433
BbrPI	CACGTG	1 cut(s)	414
BbsI	GAAGAC	1 cut(s)	354
Bbv12I	GWGCWC	1 cut(s)	139
BceAI	ACGGC	5 cut(s)	28, 51, 307, 470, 492
BclI	TGATCA	1 cut(s)	111
BcoDI	GTCTC	2 cut(s)	161, 203
BfmI	CTRYAG	2 cut(s)	187, 492
BfoI	RGCGCY	2 cut(s)	29, 44
BmeT110I	CYCGRG	1 cut(s)	206
BmiI	GGNNCC	2 cut(s)	42, 295
BmsI	GCATC	1 cut(s)	13
BpiI	GAAGAC	1 cut(s)	354
Bpu14I	TTCGAA	1 cut(s)	345
BsaAI	YACGTR	1 cut(s)	414
BsaHI	GRCGYC	2 cut(s)	41, 212
BsaI	GGTCTC	1 cut(s)	161
BsaJI	CCNNGG	1 cut(s)	16
BsaXI	ACNNNNNCTCC	4 cut(s)	345, 375, 515, 545
Bsc4I	CCNNNNNNNGG	1 cut(s)	283
Bse118I	RCCGGY	2 cut(s)	245, 535
BseDI	CCNNGG	1 cut(s)	16
BseLI	CCNNNNNNNGG	1 cut(s)	283
BseMII	CTCAG	1 cut(s)	452
BsePI	GCGCGC	1 cut(s)	147
BseRI	GAGGAG	4 cut(s)	5, 421, 539, 542
Bsh1236I	CGCG	2 cut(s)	149, 232
Bsh1285I	CGRYCG	1 cut(s)	168
BshFI	GGCC	5 cut(s)	89, 125, 239, 314, 545
BshNI	GGYRCC	1 cut(s)	40
BsiEI	CGRYCG	1 cut(s)	168
BsiHKAI	GWGCWC	1 cut(s)	139
BsiHKCI	CYCGRG	1 cut(s)	206
BsiSI	CCGG	5 cut(s)	215, 246, 297, 311, 536
BslI	CCNNNNNNNGG	1 cut(s)	283
BsmAI	GTCTC	2 cut(s)	161, 203
BsmBI	CGTCTC	1 cut(s)	203
BsmI	GAATGC	1 cut(s)	326
BsnI	GGCC	5 cut(s)	89, 125, 239, 314, 545
Bso31I	GGTCTC	1 cut(s)	161
BsoBI	CYCGRG	1 cut(s)	206
Bsp119I	TTCGAA	1 cut(s)	345
Bsp1286I	GDGCHC	1 cut(s)	139
Bsp143I	GATC	2 cut(s)	111, 165
BspANI	GGCC	5 cut(s)	89, 125, 239, 314, 545
BspCNI	CTCAG	1 cut(s)	453
BspFNI	CGCG	2 cut(s)	149, 232
BspLI	GGNNCC	2 cut(s)	42, 295
BspMAI	CTGCAG	2 cut(s)	191, 496
BspQI	GCTCTTC	1 cut(s)	251
BspT104I	TTCGAA	1 cut(s)	345
BspT107I	GGYRCC	1 cut(s)	40
BspTNI	GGTCTC	1 cut(s)	161
BsrFI	RCCGGY	2 cut(s)	245, 535
BssAI	RCCGGY	2 cut(s)	245, 535
BssECI	CCNNGG	1 cut(s)	16
BssHII	GCGCGC	1 cut(s)	147
BssMI	GATC	2 cut(s)	111, 165
BssNI	GRCGYC	2 cut(s)	41, 212
BssSI	CACGAG	1 cut(s)	433
Bst2BI	CACGAG	1 cut(s)	433
Bst6I	CTCTTC	1 cut(s)	251
BstACI	GRCGYC	2 cut(s)	41, 212
BstAPI	GCANNNNNTGC	1 cut(s)	457
BstBAI	YACGTR	1 cut(s)	414
BstBI	TTCGAA	1 cut(s)	345
BstC8I	GCNNGC	3 cut(s)	149, 222, 560
BstDEI	CTNAG	1 cut(s)	461
BstFNI	CGCG	2 cut(s)	149, 232
BstH2I	RGCGCY	2 cut(s)	29, 44
BstHHI	GCGC	6 cut(s)	28, 43, 149, 151, 179, 232
BstKTI	GATC	2 cut(s)	114, 168
BstMAI	GTCTC	2 cut(s)	161, 203
BstMBI	GATC	2 cut(s)	111, 165
BstMCI	CGRYCG	1 cut(s)	168
BstMWI	GCNNNNNNNGC	6 cut(s)	183, 236, 457, 488, 491, 518
BstSFI	CTRYAG	2 cut(s)	187, 492
BstUI	CGCG	2 cut(s)	149, 232
BstV2I	GAAGAC	1 cut(s)	354
BstXI	CCANNNNNNTGG	1 cut(s)	566
BsuRI	GGCC	5 cut(s)	89, 125, 239, 314, 545
Cac8I	GCNNGC	3 cut(s)	149, 222, 560
CfoI	GCGC	6 cut(s)	28, 43, 149, 151, 179, 232
Cfr10I	RCCGGY	2 cut(s)	245, 535
CseI	GACGC	1 cut(s)	220
Csp6I	GTAC	2 cut(s)	289, 465
CviAII	CATG	2 cut(s)	106, 395
CviQI	GTAC	2 cut(s)	289, 465
DdeI	CTNAG	1 cut(s)	461
DinI	GGCGCC	1 cut(s)	42
DpnI	GATC	2 cut(s)	113, 167
DpnII	GATC	2 cut(s)	111, 165
EaeI	YGGCCR	3 cut(s)	237, 312, 543
Eam1104I	CTCTTC	1 cut(s)	251
EarI	CTCTTC	1 cut(s)	251
EciI	GGCGGA	1 cut(s)	536
Eco31I	GGTCTC	1 cut(s)	161
Eco47III	AGCGCT	1 cut(s)	27
Eco72I	CACGTG	1 cut(s)	414
Eco88I	CYCGRG	1 cut(s)	206
EcoT22I	ATGCAT	1 cut(s)	6
EgeI	GGCGCC	1 cut(s)	42
EheI	GGCGCC	1 cut(s)	42
Esp3I	CGTCTC	1 cut(s)	203
FaeI	CATG	2 cut(s)	109, 398
FaiI	YATR	6 cut(s)	107, 116, 145, 195, 396, 407
FatI	CATG	2 cut(s)	105, 394
FbaI	TGATCA	1 cut(s)	111
GlaI	GCGC	6 cut(s)	27, 42, 148, 150, 178, 231
HaeII	RGCGCY	2 cut(s)	29, 44
HaeIII	GGCC	5 cut(s)	89, 125, 239, 314, 545
HapII	CCGG	5 cut(s)	215, 246, 297, 311, 536
HgaI	GACGC	1 cut(s)	220
HhaI	GCGC	6 cut(s)	28, 43, 149, 151, 179, 232
Hin1I	GRCGYC	2 cut(s)	41, 212
Hin1II	CATG	2 cut(s)	109, 398
Hin6I	GCGC	6 cut(s)	26, 41, 147, 149, 177, 230
HinP1I	GCGC	6 cut(s)	26, 41, 147, 149, 177, 230
HinfI	GANTC	6 cut(s)	71, 366, 390, 497, 530, 551
HpaII	CCGG	5 cut(s)	215, 246, 297, 311, 536
HphI	GGTGA	2 cut(s)	121, 525
Hpy166II	GTNNAC	1 cut(s)	289
Hpy188I	TCNGA	3 cut(s)	20, 141, 441
Hpy188III	TCNNGA	2 cut(s)	206, 208
Hpy8I	GTNNAC	1 cut(s)	289
Hpy99I	CGWCG	1 cut(s)	49
HpyCH4IV	ACGT	1 cut(s)	413
HpyCH4V	TGCA	5 cut(s)	4, 189, 381, 405, 494
HpyF10VI	GCNNNNNNNGC	6 cut(s)	183, 236, 457, 488, 491, 518
HpyF3I	CTNAG	1 cut(s)	461
HpySE526I	ACGT	1 cut(s)	413
Hsp92I	GRCGYC	2 cut(s)	41, 212
Hsp92II	CATG	2 cut(s)	109, 398
HspAI	GCGC	6 cut(s)	26, 41, 147, 149, 177, 230
KasI	GGCGCC	1 cut(s)	40
Ksp22I	TGATCA	1 cut(s)	111
Kzo9I	GATC	2 cut(s)	111, 165
LguI	GCTCTTC	1 cut(s)	251
LmnI	GCTCC	2 cut(s)	217, 299
LpnPI	CCDG	9 cut(s)	206, 228, 259, 310, 324, 549, 568, 572, 578
LweI	GCATC	1 cut(s)	13
MaeII	ACGT	1 cut(s)	413
MaeIII	GTNAC	1 cut(s)	531
MalI	GATC	2 cut(s)	113, 167
MboI	GATC	2 cut(s)	111, 165
MboII	GAAGA	3 cut(s)	265, 268, 359
MfeI	CAATTG	1 cut(s)	279
MhlI	GDGCHC	1 cut(s)	139
MluCI	AATT	2 cut(s)	279, 442
Mly113I	GGCGCC	1 cut(s)	41
MlyI	GAGTC	3 cut(s)	375, 539, 560
MnlI	CCTC	8 cut(s)	26, 79, 191, 261, 393, 442, 517, 520
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	1 cut(s)	360
MseI	TTAA	2 cut(s)	81, 601
MslI	CAYNNNNRTG	1 cut(s)	104
MspA1I	CMGCKG	4 cut(s)	174, 186, 482, 542
MspI	CCGG	5 cut(s)	215, 246, 297, 311, 536
MunI	CAATTG	1 cut(s)	279
Mva1269I	GAATGC	1 cut(s)	326
MvnI	CGCG	2 cut(s)	149, 232
MwoI	GCNNNNNNNGC	6 cut(s)	183, 236, 457, 488, 491, 518
NarI	GGCGCC	1 cut(s)	41
NdeII	GATC	2 cut(s)	111, 165
NlaIII	CATG	2 cut(s)	109, 398
NlaIV	GGNNCC	2 cut(s)	42, 295
NmuCI	GTSAC	1 cut(s)	531
NsiI	ATGCAT	1 cut(s)	6
NspV	TTCGAA	1 cut(s)	345
PaeR7I	CTCGAG	1 cut(s)	206
PauI	GCGCGC	1 cut(s)	147
PciSI	GCTCTTC	1 cut(s)	251
PcsI	WCGNNNNNNNCGW	3 cut(s)	41, 297, 431
PctI	GAATGC	1 cut(s)	326
PdmI	GAANNNNTTC	1 cut(s)	360
PfeI	GAWTC	3 cut(s)	71, 390, 497
Ple19I	CGATCG	1 cut(s)	168
PleI	GAGTC	3 cut(s)	374, 538, 559
PluTI	GGCGCC	1 cut(s)	44
PmaCI	CACGTG	1 cut(s)	414
PmlI	CACGTG	1 cut(s)	414
PpsI	GAGTC	3 cut(s)	374, 538, 559
Ppu21I	YACGTR	1 cut(s)	414
PspCI	CACGTG	1 cut(s)	414
PspN4I	GGNNCC	2 cut(s)	42, 295
PstI	CTGCAG	2 cut(s)	191, 496
PteI	GCGCGC	1 cut(s)	147
PvuI	CGATCG	1 cut(s)	168
PvuII	CAGCTG	1 cut(s)	482
RsaI	GTAC	2 cut(s)	290, 466
RsaNI	GTAC	2 cut(s)	289, 465
RseI	CAYNNNNRTG	1 cut(s)	104
SapI	GCTCTTC	1 cut(s)	251
SaqAI	TTAA	2 cut(s)	81, 601
Sau3AI	GATC	2 cut(s)	111, 165
SchI	GAGTC	3 cut(s)	375, 539, 560
SduI	GDGCHC	1 cut(s)	139
SetI	ASST	6 cut(s)	200, 404, 416, 484, 514, 568
SfaNI	GCATC	1 cut(s)	13
SfcI	CTRYAG	2 cut(s)	187, 492
SfoI	GGCGCC	1 cut(s)	42
Sfr274I	CTCGAG	1 cut(s)	206
SfuI	TTCGAA	1 cut(s)	345
SlaI	CTCGAG	1 cut(s)	206
SmiMI	CAYNNNNRTG	1 cut(s)	104
SmlI	CTYRAG	1 cut(s)	206
SmoI	CTYRAG	1 cut(s)	206
Sse9I	AATT	2 cut(s)	279, 442
SspDI	GGCGCC	1 cut(s)	40
SspI	AATATT	1 cut(s)	307
TaiI	ACGT	1 cut(s)	416
TaqI	TCGA	7 cut(s)	7, 96, 164, 207, 345, 364, 588
TasI	AATT	2 cut(s)	279, 442
TauI	GCSGC	7 cut(s)	41, 125, 227, 242, 518, 521, 545
TfiI	GAWTC	3 cut(s)	71, 390, 497
Tru1I	TTAA	2 cut(s)	81, 601
Tru9I	TTAA	2 cut(s)	81, 601
TseFI	GTSAC	1 cut(s)	531
Tsp45I	GTSAC	1 cut(s)	531
TspDTI	ATGAA	1 cut(s)	67
TspGWI	ACGGA	3 cut(s)	353, 366, 402
XhoI	CTCGAG	1 cut(s)	206
XmnI	GAANNNNTTC	1 cut(s)	360
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.