AT5G02750

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011203
Physical Location & Seq
Forward (+)
619865 .. 621180
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02750.1

Sequence Viewer

Length: 852 bp
ATGGAAGACGAAAACACCACAATCATCATGGCGTCACTCTCTGCTCTTTCTCCTTCACACCTCACGAATCTGACTCACTCCATTCTCTCTATCTCCCACCACCATCGCCGCCGCCTCGGTGCAGTCCTCTCCTCTCCGACCCTTTTCTCCCTCACTCTCCGCCACCTCCTCTCTCTATCTCTTCCTGACAAAACTCACCTCATCGCCAACCATCTTCTCTCCCTCCTCCATCCTCTTCTCATCCACCGTAAACACCACTCCTCCTACGCCGTCACCATGAAGCTCCGGGACCTAGACGCGGTGGTCCTCCTCCTCTTTCTCTGCGAAACCCACCAGCTTCATCCAGATGTTCTAGAAGCTTCCGCTGATAATTGGCGGGAAATTCTCGGTAATACGTACTCTAATAATATGTTAAGTAATAATTCCGGTCTTTGGACTTGCGATGCCGGAATCTTGATGCCGTACATAGAAACCCTAGTTAGATGCAAAAGATTTGTTGACATAATGGGAGGCTATAACCATCTTCGTCGAAGAGATCAAAAAGAAGGCTACCAAGTTCCAGCCGCTAGAGCGGCTGTGGTGGCGTTACGGGCGGTTGAGGTTTTCAACGTCGCCGCTTCAAACGCCGGAGAAGTAGAATGCGTGATATGCAAGGAAGAGATGAGCGAAGGAAGAGATGTGTGTGAAATGCCATGTCAGCATTTCTTTCACTGGAAGTGTATCTTGCCGTGGCTAAGCAAGAAGAACACGTGTCCGTTCTGCAGGTTTCAGCTTCCCACCGACGATGTCTTCTCAGAGATCCAACGGTTATGGGAGATCCTCGTCAAGAGTAGCGAGTTACACGTGGCGTAA

Protein Analysis

283

Amino Acids

32.15

Weight (kDa)

6.8

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 211 - 255 1.7e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 213 - 255 6.8e-15 Ring finger domain
zf-C3HC4_2 PF13923 213 - 254 2.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 213 - 241 9.4e-08 RING-like zinc finger
zf-C3HC4 PF00097 214 - 254 1e-05 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 753
AccBSI CCGCTC 1 cut(s) 572
AccII CGCG 1 cut(s) 299
AclWI GGATC 2 cut(s) 793, 811
AcsI RAATTY 1 cut(s) 381
AcvI CACGTG 2 cut(s) 750, 844
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 398, 464
AfiI CCNNNNNNNGG 2 cut(s) 298, 432
AflIII ACRYGT 3 cut(s) 747, 749, 841
AgsI TTSAA 2 cut(s) 607, 621
AluBI AGCT 4 cut(s) 283, 337, 359, 772
AluI AGCT 4 cut(s) 283, 337, 359, 772
AlwI GGATC 2 cut(s) 793, 811
ApoI RAATTY 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 289, 304
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 188, 265
AvaII GGWCC 2 cut(s) 289, 304
BbrPI CACGTG 2 cut(s) 750, 844
BbsI GAAGAC 2 cut(s) 12, 781
BccI CCATC 4 cut(s) 111, 219, 237, 528
BceAI ACGGC 3 cut(s) 254, 445, 712
BcnI CCSGG 1 cut(s) 287
BfaI CTAG 4 cut(s) 293, 353, 476, 567
BfmI CTRYAG 1 cut(s) 760
BfuAI ACCTGC 1 cut(s) 753
BisI GCNGC 5 cut(s) 109, 112, 564, 573, 615
BlpI GCTNAGC 1 cut(s) 734
BlsI GCNGC 5 cut(s) 110, 113, 565, 574, 616
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 2 cut(s) 289, 304
BmgT120I GGNCC 2 cut(s) 289, 304
BmiI GGNNCC 1 cut(s) 290
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 433, 447, 473
BpiI GAAGAC 2 cut(s) 12, 781
Bpu1102I GCTNAGC 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 287
BsaAI YACGTR 3 cut(s) 396, 750, 844
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 115, 728
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 432
Bse1I ACTGG 1 cut(s) 716
BseDI CCNNGG 2 cut(s) 115, 728
BseGI GGATG 3 cut(s) 229, 240, 340
BseLI CCNNNNNNNGG 2 cut(s) 298, 432
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 1 cut(s) 716
BseRI GAGGAG 6 cut(s) 121, 158, 215, 250, 299, 302
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 286, 426, 447, 627
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 2 cut(s) 298, 432
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 1 cut(s) 644
Bsp143I GATC 3 cut(s) 535, 798, 816
Bsp1720I GCTNAGC 1 cut(s) 734
BspCNI CTCAG 1 cut(s) 806
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 290
BspMAI CTGCAG 1 cut(s) 764
BspMI ACCTGC 1 cut(s) 753
BspPI GGATC 2 cut(s) 793, 811
BsrBI CCGCTC 1 cut(s) 572
BsrI ACTGG 1 cut(s) 716
BssECI CCNNGG 2 cut(s) 115, 728
BssMI GATC 3 cut(s) 535, 798, 816
BssNI GRCGYC 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 248, 807
Bst6I CTCTTC 5 cut(s) 186, 240, 526, 651, 667
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 3 cut(s) 396, 750, 844
BstDEI CTNAG 2 cut(s) 734, 793
BstDSI CCRYGG 1 cut(s) 728
BstF5I GGATG 3 cut(s) 229, 240, 340
BstFNI CGCG 1 cut(s) 299
BstKTI GATC 3 cut(s) 538, 801, 819
BstMBI GATC 3 cut(s) 535, 798, 816
BstMWI GCNNNNNNNGC 7 cut(s) 569, 572, 581, 590, 623, 648, 697
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 760
BstSNI TACGTA 1 cut(s) 396
BstUI CGCG 1 cut(s) 299
BstV2I GAAGAC 2 cut(s) 12, 781
BstX2I RGATCY 2 cut(s) 798, 816
BstYI RGATCY 2 cut(s) 798, 816
BtgI CCRYGG 1 cut(s) 728
BtgZI GCGATG 3 cut(s) 89, 187, 456
BtsCI GGATG 3 cut(s) 229, 240, 340
BtsIMutI CAGTG 1 cut(s) 709
BveI ACCTGC 1 cut(s) 753
Cfr13I GGNCC 2 cut(s) 289, 304
CseI GACGC 2 cut(s) 21, 305
Csp6I GTAC 2 cut(s) 397, 463
CviAII CATG 3 cut(s) 28, 277, 693
CviJI RGCY 9 cut(s) 283, 337, 359, 513, 549, 563, 575, 733, 772
CviKI_1 RGCY 9 cut(s) 283, 337, 359, 513, 549, 563, 575, 733, 772
CviQI GTAC 2 cut(s) 397, 463
DdeI CTNAG 2 cut(s) 734, 793
DpnI GATC 3 cut(s) 537, 800, 818
DpnII GATC 3 cut(s) 535, 798, 816
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
Eam1104I CTCTTC 5 cut(s) 186, 240, 526, 651, 667
EarI CTCTTC 5 cut(s) 186, 240, 526, 651, 667
EciI GGCGGA 1 cut(s) 149
Eco105I TACGTA 1 cut(s) 396
Eco47I GGWCC 2 cut(s) 289, 304
Eco72I CACGTG 2 cut(s) 750, 844
EcoO109I RGGNCCY 1 cut(s) 289
FaeI CATG 3 cut(s) 31, 280, 696
FaiI YATR 9 cut(s) 29, 278, 410, 467, 503, 516, 649, 694, 811
FalI AAGNNNNNCTT 2 cut(s) 707, 739
FaqI GGGAC 1 cut(s) 302
FatI CATG 3 cut(s) 27, 276, 692
FauI CCCGC 1 cut(s) 369
Fnu4HI GCNGC 5 cut(s) 109, 112, 564, 573, 615
FokI GGATG 3 cut(s) 216, 227, 327
Fsp4HI GCNGC 5 cut(s) 109, 112, 564, 573, 615
FspBI CTAG 4 cut(s) 293, 353, 476, 567
GluI GCNGC 5 cut(s) 109, 112, 564, 573, 615
HapII CCGG 4 cut(s) 286, 426, 447, 627
HgaI GACGC 2 cut(s) 21, 305
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 3 cut(s) 31, 280, 696
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 3 cut(s) 67, 73, 450
HpaII CCGG 4 cut(s) 286, 426, 447, 627
HphI GGTGA 2 cut(s) 188, 265
Hpy166II GTNNAC 2 cut(s) 251, 499
Hpy188I TCNGA 3 cut(s) 72, 138, 796
Hpy188III TCNNGA 6 cut(s) 64, 185, 344, 353, 454, 826
Hpy8I GTNNAC 2 cut(s) 251, 499
Hpy99I CGWCG 3 cut(s) 531, 614, 785
HpyAV CCTTC 3 cut(s) 63, 539, 662
HpyCH4III ACNGT 2 cut(s) 248, 807
HpyCH4IV ACGT 4 cut(s) 395, 609, 749, 843
HpyCH4V TGCA 4 cut(s) 122, 486, 651, 762
HpyF10VI GCNNNNNNNGC 7 cut(s) 569, 572, 581, 590, 623, 648, 697
HpyF3I CTNAG 2 cut(s) 734, 793
HpySE526I ACGT 4 cut(s) 395, 609, 749, 843
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 3 cut(s) 31, 280, 696
Kzo9I GATC 3 cut(s) 535, 798, 816
LmnI GCTCC 1 cut(s) 288
LweI GCATC 3 cut(s) 433, 447, 473
MaeI CTAG 4 cut(s) 293, 353, 476, 567
MaeII ACGT 4 cut(s) 395, 609, 749, 843
MaeIII GTNAC 4 cut(s) 33, 271, 585, 837
MalI GATC 3 cut(s) 537, 800, 818
MbiI CCGCTC 1 cut(s) 572
MboI GATC 3 cut(s) 535, 798, 816
MflI RGATCY 2 cut(s) 798, 816
MluCI AATT 3 cut(s) 370, 381, 421
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 2 cut(s) 161, 826
MseI TTAA 1 cut(s) 413
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 4 cut(s) 286, 426, 447, 627
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 644
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 7 cut(s) 569, 572, 581, 590, 623, 648, 697
NciI CCSGG 1 cut(s) 287
NdeII GATC 3 cut(s) 535, 798, 816
NlaIII CATG 3 cut(s) 31, 280, 696
NlaIV GGNNCC 1 cut(s) 290
NmuCI GTSAC 2 cut(s) 33, 271
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 2 cut(s) 67, 450
PflFI GACNNNGTC 1 cut(s) 785
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 5 cut(s) 110, 113, 565, 574, 616
PleI GAGTC 1 cut(s) 67
PmaCI CACGTG 2 cut(s) 750, 844
PmlI CACGTG 2 cut(s) 750, 844
PpsI GAGTC 1 cut(s) 67
Ppu21I YACGTR 3 cut(s) 396, 750, 844
PpuMI RGGWCCY 1 cut(s) 289
Psp5II RGGWCCY 1 cut(s) 289
PspCI CACGTG 2 cut(s) 750, 844
PspN4I GGNNCC 1 cut(s) 290
PspPI GGNCC 2 cut(s) 289, 304
PspPPI RGGWCCY 1 cut(s) 289
PstI CTGCAG 1 cut(s) 764
PsuI RGATCY 2 cut(s) 798, 816
PsyI GACNNNGTC 1 cut(s) 785
RsaI GTAC 2 cut(s) 398, 464
RsaNI GTAC 2 cut(s) 397, 463
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 1 cut(s) 413
SatI GCNGC 5 cut(s) 109, 112, 564, 573, 615
Sau3AI GATC 3 cut(s) 535, 798, 816
Sau96I GGNCC 2 cut(s) 289, 304
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 287
SfaNI GCATC 3 cut(s) 433, 447, 473
SfcI CTRYAG 1 cut(s) 760
SinI GGWCC 2 cut(s) 289, 304
SmiMI CAYNNNNRTG 1 cut(s) 345
SnaBI TACGTA 1 cut(s) 396
Sse9I AATT 3 cut(s) 370, 381, 421
SspMI CTAG 4 cut(s) 293, 353, 476, 567
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 248, 807
TaiI ACGT 4 cut(s) 398, 612, 752, 846
TaqI TCGA 1 cut(s) 529
TasI AATT 3 cut(s) 370, 381, 421
TauI GCSGC 5 cut(s) 111, 114, 566, 575, 617
TfiI GAWTC 2 cut(s) 67, 450
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 1 cut(s) 716
TseFI GTSAC 2 cut(s) 33, 271
Tsp45I GTSAC 2 cut(s) 33, 271
TspDTI ATGAA 2 cut(s) 293, 329
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 1 cut(s) 716
Tth111I GACNNNGTC 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 289, 304
XapI RAATTY 1 cut(s) 381
XbaI TCTAGA 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 4 cut(s) 293, 353, 476, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.