Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011203

Physical Location & Seq

Genomic Coordinates Reverse (-)

20515836 .. 20516663

Gene Structure 828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1130000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 828 bp

ATGGAAGAAACAGCAACAGCAACGGCAACCATCATGGCTGCTCTGGCCACCCTCACCCCACCCCAACTCTCACATCTCACCCACACCATCCTCTCCCACACCCACCACCACCACCACCGTCTCTCCTCCCTCCTCTCCTCCCCCATCCTCTTCTCTCTCACCCTCCACCGCCTCAACTCCCTCCCCCTCCCCCACAAGACTCTCCTCATCGCCAACCACCTCCTCTCCTCCCTCTACCACCTCACACTCCACTTCCACCCTTGCACCAATCCACCACCGCCACGCGTCGTCAGAAAAAGAGATCTCGACTCCGTCCTGCTCCTCCTCTTACTCTGCGAAGTACACCAGCACAACCCGGAAGCCCTCCAAGCACCAACTATAAAATGGCGCGAAATTTTAAGCAAGTTGTACTCCGACAACATGCTAACAGTTTCCGGCATCGGAGTCTACAACGGGTCGGCTTTGGTTTCTTATATTGAGGTGCTGACGAGGTGCTTGAGATTTGTCAGCGTGATGGGCTTTTGCTATGGCGGGAAGGCAGGGAGAGAAGTAGCGGCATCACCAGCAGCGGTGGTGGCGCTGCCGTCGGTGAAGGTGAGCAGCGGCGGAAGCGAATGCATGATATGTAAAGAAGAGATGAGAGAGGACAGAGACGTGTGTGAACTGCCGTGCCGGCATTTGTTTCACTGGATGTGTATTTTGCGGTGGCTGAGGAAGAGGAACACTTGTCCGTGCTGCCGGTTTACGCTTCCCACGGACGATGTGTTCGGGGAGATCCAACGGCTGTGGGAGATTTTGGTTAAGATGGGTGAGAAGGAATTAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009501 GO:0009536 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

276

Amino Acids

30.96

Weight (kDa)

8.46

Isoelectric Point (pI)

51.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	204 - 247	2.6e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	205 - 247	3.6e-13	Ring finger domain
zf-C3HC4_2	PF13923	205 - 246	8.4e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	205 - 233	1.8e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011203)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02750
fragaria_vesca	FvH4_6g14890
malus_domestica	MD04G1110800.v1.1 MD12G1130000.v1.1 MD15G1018600.v1.1
pyrus_communis	pycom04g10430 pycom12g12610
rosa_chinensis	RchiOBHm_Chr3g0468101
rosa_laevigata	RLG00000024449
rosa_multiflora	Rmu_co8333667.1_g000001 Rmu_sc0003659.1_g000014 Rmu_sc0012651.1_g000009 Rmu_sc0039822.1_g000001
rosa_roxburghii	Rroxscaffold_6G00413050
rosa_rugosa	Rorug03G0092700
rosa_samantha	Rh3AG141100 Rh3BG162600 Rh3CG162400 Rh3DG163100
rosa_wichuraiana	Rw3G013330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	447
AccII	CGCG	2 cut(s)	285, 390
AciI	CCGC	8 cut(s)	169, 278, 531, 554, 569, 603, 606, 703
AclWI	GGATC	1 cut(s)	769
AcoI	YGGCCR	1 cut(s)	45
AcsI	RAATTY	1 cut(s)	393
AfaI	GTAC	2 cut(s)	342, 410
AflIII	ACRYGT	2 cut(s)	283, 654
AjiI	CACGTC	1 cut(s)	655
AloI	GAACNNNNNNTCC	2 cut(s)	749, 781
Alw26I	GTCTC	2 cut(s)	125, 645
AlwI	GGATC	1 cut(s)	769
AoxI	GGCC	1 cut(s)	45
ApeKI	GCWGC	5 cut(s)	38, 566, 580, 600, 735
ApoI	RAATTY	1 cut(s)	393
ArsI	GACNNNNNNTTYG	2 cut(s)	749, 781
AspLEI	GCGC	2 cut(s)	390, 580
AsuC2I	CCSGG	1 cut(s)	356
AsuHPI	GGTGA	7 cut(s)	46, 70, 151, 552, 601, 607, 821
BalI	TGGCCA	1 cut(s)	47
BbvCI	CCTCAGC	1 cut(s)	710
BbvI	GCAGC	5 cut(s)	25, 567, 578, 612, 722
BccI	CCATC	5 cut(s)	38, 95, 152, 508, 799
BceAI	ACGGC	4 cut(s)	39, 568, 652, 797
BcnI	CCSGG	1 cut(s)	356
BcoDI	GTCTC	2 cut(s)	125, 645
BfoI	RGCGCY	1 cut(s)	581
BglI	GCCNNNNNGGC	1 cut(s)	673
BglII	AGATCT	1 cut(s)	301
BisI	GCNGC	7 cut(s)	39, 555, 567, 581, 601, 604, 736
BlsI	GCNGC	7 cut(s)	40, 556, 568, 582, 602, 605, 737
Bme1390I	CCNGG	1 cut(s)	356
BmgBI	CACGTC	1 cut(s)	655
BmrFI	CCNGG	1 cut(s)	356
BmsI	GCATC	2 cut(s)	447, 566
Bpu10I	CCTNAGC	1 cut(s)	710
BpuEI	CTTGAG	1 cut(s)	517
BpuMI	CCSGG	1 cut(s)	356
BsaJI	CCNNGG	1 cut(s)	753
BsaXI	ACNNNNNCTCC	4 cut(s)	107, 137, 209, 239
Bse118I	RCCGGY	2 cut(s)	672, 738
Bse1I	ACTGG	1 cut(s)	692
BseDI	CCNNGG	1 cut(s)	753
BseGI	GGATG	3 cut(s)	87, 144, 696
BseMII	CTCAG	1 cut(s)	701
BseNI	ACTGG	1 cut(s)	692
BseRI	GAGGAG	8 cut(s)	115, 122, 127, 194, 212, 217, 311, 314
BseXI	GCAGC	5 cut(s)	25, 567, 578, 612, 722
Bsh1236I	CGCG	2 cut(s)	285, 390
BshFI	GGCC	1 cut(s)	47
BsiSI	CCGG	4 cut(s)	356, 435, 673, 739
BsmAI	GTCTC	2 cut(s)	125, 645
BsmBI	CGTCTC	2 cut(s)	125, 645
BsmI	GAATGC	1 cut(s)	620
BsnI	GGCC	1 cut(s)	47
Bsp143I	GATC	2 cut(s)	301, 774
BspACI	CCGC	8 cut(s)	169, 278, 531, 554, 569, 603, 606, 703
BspANI	GGCC	1 cut(s)	47
BspCNI	CTCAG	1 cut(s)	702
BspFNI	CGCG	2 cut(s)	285, 390
BspPI	GGATC	1 cut(s)	769
BsrFI	RCCGGY	2 cut(s)	672, 738
BsrI	ACTGG	1 cut(s)	692
BssAI	RCCGGY	2 cut(s)	672, 738
BssECI	CCNNGG	1 cut(s)	753
BssMI	GATC	2 cut(s)	301, 774
Bst4CI	ACNGT	2 cut(s)	119, 430
Bst6I	CTCTTC	3 cut(s)	155, 627, 710
BstC8I	GCNNGC	1 cut(s)	674
BstDEI	CTNAG	1 cut(s)	710
BstDSI	CCRYGG	1 cut(s)	753
BstF5I	GGATG	3 cut(s)	87, 144, 696
BstFNI	CGCG	2 cut(s)	285, 390
BstH2I	RGCGCY	1 cut(s)	581
BstHHI	GCGC	2 cut(s)	390, 580
BstKTI	GATC	2 cut(s)	304, 777
BstMAI	GTCTC	2 cut(s)	125, 645
BstMBI	GATC	2 cut(s)	301, 774
BstMWI	GCNNNNNNNGC	7 cut(s)	44, 368, 516, 563, 575, 609, 673
BstNSI	RCATGY	1 cut(s)	424
BstSCI	CCNGG	1 cut(s)	354
BstUI	CGCG	2 cut(s)	285, 390
BstV1I	GCAGC	5 cut(s)	25, 567, 578, 612, 722
BstX2I	RGATCY	2 cut(s)	301, 774
BstYI	RGATCY	2 cut(s)	301, 774
BsuRI	GGCC	1 cut(s)	47
BtgI	CCRYGG	1 cut(s)	753
BtgZI	GCGATG	1 cut(s)	193
BtrI	CACGTC	1 cut(s)	655
BtsCI	GGATG	3 cut(s)	87, 144, 696
BtsIMutI	CAGTG	1 cut(s)	685
Cac8I	GCNNGC	1 cut(s)	674
CfoI	GCGC	2 cut(s)	390, 580
Cfr10I	RCCGGY	2 cut(s)	672, 738
CseI	GACGC	1 cut(s)	274
Csp6I	GTAC	2 cut(s)	341, 409
CspCI	CAANNNNNGTGG	2 cut(s)	767, 802
CviAII	CATG	3 cut(s)	34, 421, 619
CviJI	RGCY	7 cut(s)	38, 47, 362, 461, 519, 709, 784
CviKI_1	RGCY	7 cut(s)	38, 47, 362, 461, 519, 709, 784
CviQI	GTAC	2 cut(s)	341, 409
DdeI	CTNAG	1 cut(s)	710
DpnI	GATC	2 cut(s)	303, 776
DpnII	GATC	2 cut(s)	301, 774
EaeI	YGGCCR	1 cut(s)	45
Eam1104I	CTCTTC	3 cut(s)	155, 627, 710
EarI	CTCTTC	3 cut(s)	155, 627, 710
EciI	GGCGGA	1 cut(s)	621
EcoT22I	ATGCAT	1 cut(s)	620
Esp3I	CGTCTC	2 cut(s)	125, 645
FaeI	CATG	3 cut(s)	37, 424, 622
FaiI	YATR	7 cut(s)	35, 380, 422, 474, 528, 620, 625
FatI	CATG	3 cut(s)	33, 420, 618
FauI	CCCGC	1 cut(s)	524
FblI	GTMKAC	1 cut(s)	447
Fnu4HI	GCNGC	7 cut(s)	39, 555, 567, 581, 601, 604, 736
FokI	GGATG	3 cut(s)	74, 131, 703
Fsp4HI	GCNGC	7 cut(s)	39, 555, 567, 581, 601, 604, 736
GlaI	GCGC	2 cut(s)	389, 579
GluI	GCNGC	7 cut(s)	39, 555, 567, 581, 601, 604, 736
HaeII	RGCGCY	1 cut(s)	581
HaeIII	GGCC	1 cut(s)	47
HapII	CCGG	4 cut(s)	356, 435, 673, 739
HgaI	GACGC	1 cut(s)	274
HhaI	GCGC	2 cut(s)	390, 580
Hin1II	CATG	3 cut(s)	37, 424, 622
Hin6I	GCGC	2 cut(s)	388, 578
HinP1I	GCGC	2 cut(s)	388, 578
HinfI	GANTC	3 cut(s)	199, 308, 444
HpaII	CCGG	4 cut(s)	356, 435, 673, 739
HphI	GGTGA	7 cut(s)	46, 70, 151, 552, 601, 607, 821
Hpy166II	GTNNAC	4 cut(s)	343, 448, 662, 744
Hpy188I	TCNGA	3 cut(s)	293, 415, 443
Hpy188III	TCNNGA	1 cut(s)	305
Hpy8I	GTNNAC	4 cut(s)	343, 448, 662, 744
Hpy99I	CGWCG	2 cut(s)	290, 589
HpyAV	CCTTC	3 cut(s)	529, 586, 808
HpyCH4III	ACNGT	2 cut(s)	119, 430
HpyCH4IV	ACGT	1 cut(s)	654
HpyCH4V	TGCA	2 cut(s)	264, 618
HpyF10VI	GCNNNNNNNGC	7 cut(s)	44, 368, 516, 563, 575, 609, 673
HpyF3I	CTNAG	1 cut(s)	710
HpySE526I	ACGT	1 cut(s)	654
Hsp92II	CATG	3 cut(s)	37, 424, 622
HspAI	GCGC	2 cut(s)	388, 578
KroI	GCCGGC	1 cut(s)	672
KroNI	GCCGGC	1 cut(s)	674
Kzo9I	GATC	2 cut(s)	301, 774
LmnI	GCTCC	1 cut(s)	324
Lsp1109I	GCAGC	5 cut(s)	25, 567, 578, 612, 722
LweI	GCATC	2 cut(s)	447, 566
MaeII	ACGT	1 cut(s)	654
MalI	GATC	2 cut(s)	303, 776
MboI	GATC	2 cut(s)	301, 774
MboII	GAAGA	4 cut(s)	17, 142, 644, 727
MflI	RGATCY	2 cut(s)	301, 774
MlsI	TGGCCA	1 cut(s)	47
MluCI	AATT	2 cut(s)	393, 818
MluI	ACGCGT	1 cut(s)	283
MluNI	TGGCCA	1 cut(s)	47
MlyI	GAGTC	3 cut(s)	193, 302, 453
MmeI	TCCRAC	2 cut(s)	438, 802
Mox20I	TGGCCA	1 cut(s)	47
Mph1103I	ATGCAT	1 cut(s)	620
MroNI	GCCGGC	1 cut(s)	672
MscI	TGGCCA	1 cut(s)	47
MseI	TTAA	2 cut(s)	398, 801
Msp20I	TGGCCA	1 cut(s)	47
MspA1I	CMGCKG	2 cut(s)	569, 603
MspI	CCGG	4 cut(s)	356, 435, 673, 739
MspR9I	CCNGG	1 cut(s)	356
Mva1269I	GAATGC	1 cut(s)	620
MvnI	CGCG	2 cut(s)	285, 390
MwoI	GCNNNNNNNGC	7 cut(s)	44, 368, 516, 563, 575, 609, 673
NaeI	GCCGGC	1 cut(s)	674
NciI	CCSGG	1 cut(s)	356
NdeII	GATC	2 cut(s)	301, 774
NgoMIV	GCCGGC	1 cut(s)	672
NlaIII	CATG	3 cut(s)	37, 424, 622
NsiI	ATGCAT	1 cut(s)	620
NspI	RCATGY	1 cut(s)	424
PctI	GAATGC	1 cut(s)	620
PdiI	GCCGGC	1 cut(s)	674
PflFI	GACNNNGTC	1 cut(s)	311
PkrI	GCNGC	7 cut(s)	40, 556, 568, 582, 602, 605, 737
PleI	GAGTC	3 cut(s)	193, 302, 452
PpsI	GAGTC	3 cut(s)	193, 302, 452
PsuI	RGATCY	2 cut(s)	301, 774
PsyI	GACNNNGTC	1 cut(s)	311
RsaI	GTAC	2 cut(s)	342, 410
RsaNI	GTAC	2 cut(s)	341, 409
SaqAI	TTAA	2 cut(s)	398, 801
SatI	GCNGC	7 cut(s)	39, 555, 567, 581, 601, 604, 736
Sau3AI	GATC	2 cut(s)	301, 774
SchI	GAGTC	3 cut(s)	193, 302, 453
ScrFI	CCNGG	1 cut(s)	356
SetI	ASST	6 cut(s)	222, 243, 483, 494, 597, 657
SfaNI	GCATC	2 cut(s)	447, 566
SmlI	CTYRAG	1 cut(s)	496
SmoI	CTYRAG	1 cut(s)	496
Sse9I	AATT	2 cut(s)	393, 818
SsiI	CCGC	8 cut(s)	169, 278, 531, 554, 569, 603, 606, 703
StyD4I	CCNGG	1 cut(s)	354
TaaI	ACNGT	2 cut(s)	119, 430
TaiI	ACGT	1 cut(s)	657
TaqI	TCGA	1 cut(s)	306
TasI	AATT	2 cut(s)	393, 818
TatI	WGTACW	2 cut(s)	340, 408
TauI	GCSGC	2 cut(s)	557, 606
Tru1I	TTAA	2 cut(s)	398, 801
Tru9I	TTAA	2 cut(s)	398, 801
TscAI	CASTG	1 cut(s)	692
TseI	GCWGC	5 cut(s)	38, 566, 580, 600, 735
TspGWI	ACGGA	3 cut(s)	301, 720, 770
TspRI	CASTG	1 cut(s)	692
Tth111I	GACNNNGTC	1 cut(s)	311
XapI	RAATTY	1 cut(s)	393
XceI	RCATGY	1 cut(s)	424
XcmI	CCANNNNNNNNNTGG	1 cut(s)	381
XmiI	GTMKAC	1 cut(s)	447
Zsp2I	ATGCAT	1 cut(s)	620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.