Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011203

Physical Location & Seq

Genomic Coordinates Forward (+)

19554498 .. 19555322

Gene Structure 825 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1110800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 825 bp

ATGGAAGAAACAACAGCAGCAACCGCAACCATCATGGCTGCTCTGGCCACCCTCACCCCACCCCAACTCTCAGATCTCACCCGCACCATCCTCTCCCACACCCATCACCACCACCGCCGTCTCTCCTCCCTCCTATCCTCCCCCATCCTCTTCTCTCTCACCCTCCACCGCCTCAACTGCCTCCCTCTCGCCCACAAGACTCTCCTCATCGCCAACCACCTCCTCTCCTCCCTCCACCACCTTACACTCCACTTCCAACCCTACACCAATCTACCGCCGCGGCAGGCCAGACAAAGAGACCTCGACGCCGTCCTGCTCCTCCTCTTACTCTGCGAAGTACACCAGCACAACCCTCAAGCCCTCGAAGCACCAAATGTAAAATGGCGCGAAATTCTAAGCGATTTGTACTCCGACAACATGTTAACAGTTTCCGGCATTGGGGTCTACAACGGGTCGGCTTTGGTGTCTTATATTGAGGTGCTGACAAGGTGCTTGAGATTCGTCAGCGTGATGGGTTTTTGCTGTGGCGGGAAGGCAGGGAGAGAAGTGGCGGCATCGCCAGCAGTCGTGGTGTCGCTGCCGTCAGTGGTGGTAAGCGGTGGCGGGAGCGAGTGCGTGATATGTAAAGAGGAGATGAGAGAACACAGAGATGTGTGTGAGCTGCCGTGCCGGCATTTGTTTCATTGGATGTGTATTTTGCGGTGGCTGAGGAAGAGGAACACTTGTCCATGCTGCCGGTTTACGCTTCCCACGGACGATGTGTTCGGGGAGATCCAACGGCTGTGGGAGATTTTAGTTAAGATAGGCGAGAAGGAATTAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009501 GO:0009536 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

275

Amino Acids

30.75

Weight (kDa)

7.99

Isoelectric Point (pI)

47.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	204 - 246	5e-13	Ring finger domain
zf-C3HC4_2	PF13923	204 - 245	1.6e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	204 - 246	3.2e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011203)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02750
fragaria_vesca	FvH4_6g14890
malus_domestica	MD04G1110800.v1.1 MD12G1130000.v1.1 MD15G1018600.v1.1
pyrus_communis	pycom04g10430 pycom12g12610
rosa_chinensis	RchiOBHm_Chr3g0468101
rosa_laevigata	RLG00000024449
rosa_multiflora	Rmu_co8333667.1_g000001 Rmu_sc0003659.1_g000014 Rmu_sc0012651.1_g000009 Rmu_sc0039822.1_g000001
rosa_roxburghii	Rroxscaffold_6G00413050
rosa_rugosa	Rorug03G0092700
rosa_samantha	Rh3AG141100 Rh3BG162600 Rh3CG162400 Rh3DG163100
rosa_wichuraiana	Rw3G013330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	444
AccII	CGCG	2 cut(s)	280, 387
AclWI	GGATC	1 cut(s)	766
AcoI	YGGCCR	1 cut(s)	45
AcsI	RAATTY	1 cut(s)	390
AcyI	GRCGYC	1 cut(s)	306
AfaI	GTAC	2 cut(s)	339, 407
AfiI	CCNNNNNNNGG	1 cut(s)	438
AflIII	ACRYGT	1 cut(s)	417
AloI	GAACNNNNNNTCC	2 cut(s)	746, 778
AluBI	AGCT	2 cut(s)	661, 821
AluI	AGCT	2 cut(s)	661, 821
Alw26I	GTCTC	2 cut(s)	125, 291
AlwI	GGATC	1 cut(s)	766
AoxI	GGCC	2 cut(s)	45, 285
ApeKI	GCWGC	5 cut(s)	17, 38, 577, 661, 732
ApoI	RAATTY	1 cut(s)	390
ArsI	GACNNNNNNTTYG	2 cut(s)	746, 778
AspLEI	GCGC	1 cut(s)	387
AsuHPI	GGTGA	4 cut(s)	46, 70, 98, 151
BalI	TGGCCA	1 cut(s)	47
BbvCI	CCTCAGC	1 cut(s)	707
BbvI	GCAGC	5 cut(s)	25, 29, 564, 648, 719
BccI	CCATC	5 cut(s)	38, 95, 111, 152, 505
BceAI	ACGGC	5 cut(s)	102, 293, 565, 649, 794
BcoDI	GTCTC	2 cut(s)	125, 291
BglI	GCCNNNNNGGC	1 cut(s)	670
BglII	AGATCT	1 cut(s)	73
BisI	GCNGC	8 cut(s)	18, 39, 278, 281, 552, 578, 662, 733
BlsI	GCNGC	8 cut(s)	19, 40, 279, 282, 553, 579, 663, 734
BmsI	GCATC	1 cut(s)	563
Bpu10I	CCTNAGC	1 cut(s)	707
BpuEI	CTTGAG	2 cut(s)	339, 514
BsaHI	GRCGYC	1 cut(s)	306
BsaI	GGTCTC	1 cut(s)	291
BsaJI	CCNNGG	2 cut(s)	278, 750
BsaXI	ACNNNNNCTCC	2 cut(s)	209, 239
Bsc4I	CCNNNNNNNGG	1 cut(s)	438
Bse118I	RCCGGY	2 cut(s)	669, 735
BseDI	CCNNGG	2 cut(s)	278, 750
BseGI	GGATG	3 cut(s)	87, 144, 693
BseLI	CCNNNNNNNGG	1 cut(s)	438
BseMII	CTCAG	2 cut(s)	84, 698
BseRI	GAGGAG	7 cut(s)	115, 194, 212, 217, 308, 311, 644
BseXI	GCAGC	5 cut(s)	25, 29, 564, 648, 719
Bsh1236I	CGCG	2 cut(s)	280, 387
BshFI	GGCC	2 cut(s)	47, 287
BsiSI	CCGG	3 cut(s)	432, 670, 736
BslI	CCNNNNNNNGG	1 cut(s)	438
BsmAI	GTCTC	2 cut(s)	125, 291
BsmBI	CGTCTC	1 cut(s)	125
BsnI	GGCC	2 cut(s)	47, 287
Bso31I	GGTCTC	1 cut(s)	291
Bsp143I	GATC	2 cut(s)	73, 771
BspANI	GGCC	2 cut(s)	47, 287
BspCNI	CTCAG	2 cut(s)	83, 699
BspFNI	CGCG	2 cut(s)	280, 387
BspPI	GGATC	1 cut(s)	766
BspTNI	GGTCTC	1 cut(s)	291
BsrFI	RCCGGY	2 cut(s)	669, 735
BssAI	RCCGGY	2 cut(s)	669, 735
BssECI	CCNNGG	2 cut(s)	278, 750
BssMI	GATC	2 cut(s)	73, 771
BssNI	GRCGYC	1 cut(s)	306
Bst4CI	ACNGT	1 cut(s)	427
Bst6I	CTCTTC	2 cut(s)	155, 707
BstACI	GRCGYC	1 cut(s)	306
BstC8I	GCNNGC	3 cut(s)	285, 561, 671
BstDEI	CTNAG	4 cut(s)	70, 395, 707, 822
BstDSI	CCRYGG	2 cut(s)	278, 750
BstF5I	GGATG	3 cut(s)	87, 144, 693
BstFNI	CGCG	2 cut(s)	280, 387
BstHHI	GCGC	1 cut(s)	387
BstKTI	GATC	2 cut(s)	76, 774
BstMAI	GTCTC	2 cut(s)	125, 291
BstMBI	GATC	2 cut(s)	73, 771
BstMWI	GCNNNNNNNGC	6 cut(s)	23, 44, 177, 365, 560, 670
BstNSI	RCATGY	1 cut(s)	421
BstUI	CGCG	2 cut(s)	280, 387
BstV1I	GCAGC	5 cut(s)	25, 29, 564, 648, 719
BstX2I	RGATCY	2 cut(s)	73, 771
BstYI	RGATCY	2 cut(s)	73, 771
BsuRI	GGCC	2 cut(s)	47, 287
BtgI	CCRYGG	2 cut(s)	278, 750
BtgZI	GCGATG	2 cut(s)	193, 540
BtsCI	GGATG	3 cut(s)	87, 144, 693
BtsIMutI	CAGTG	1 cut(s)	591
Cac8I	GCNNGC	3 cut(s)	285, 561, 671
CfoI	GCGC	1 cut(s)	387
Cfr10I	RCCGGY	2 cut(s)	669, 735
Cfr42I	CCGCGG	1 cut(s)	281
CseI	GACGC	1 cut(s)	314
Csp6I	GTAC	2 cut(s)	338, 406
CspCI	CAANNNNNGTGG	2 cut(s)	764, 799
CviAII	CATG	3 cut(s)	34, 418, 729
CviJI	RGCY	9 cut(s)	38, 47, 287, 359, 458, 661, 706, 781, 821
CviKI_1	RGCY	9 cut(s)	38, 47, 287, 359, 458, 661, 706, 781, 821
CviQI	GTAC	2 cut(s)	338, 406
DdeI	CTNAG	4 cut(s)	70, 395, 707, 822
DpnI	GATC	2 cut(s)	75, 773
DpnII	GATC	2 cut(s)	73, 771
EaeI	YGGCCR	1 cut(s)	45
Eam1104I	CTCTTC	2 cut(s)	155, 707
EarI	CTCTTC	2 cut(s)	155, 707
Eco31I	GGTCTC	1 cut(s)	291
Esp3I	CGTCTC	1 cut(s)	125
FaeI	CATG	3 cut(s)	37, 421, 732
FaiI	YATR	5 cut(s)	35, 419, 471, 622, 730
FatI	CATG	3 cut(s)	33, 417, 728
FauI	CCCGC	3 cut(s)	89, 521, 596
FblI	GTMKAC	1 cut(s)	444
Fnu4HI	GCNGC	8 cut(s)	18, 39, 278, 281, 552, 578, 662, 733
FokI	GGATG	3 cut(s)	74, 131, 700
Fsp4HI	GCNGC	8 cut(s)	18, 39, 278, 281, 552, 578, 662, 733
GlaI	GCGC	1 cut(s)	386
GluI	GCNGC	8 cut(s)	18, 39, 278, 281, 552, 578, 662, 733
HaeIII	GGCC	2 cut(s)	47, 287
HapII	CCGG	3 cut(s)	432, 670, 736
HgaI	GACGC	1 cut(s)	314
HhaI	GCGC	1 cut(s)	387
Hin1I	GRCGYC	1 cut(s)	306
Hin1II	CATG	3 cut(s)	37, 421, 732
Hin6I	GCGC	1 cut(s)	385
HinP1I	GCGC	1 cut(s)	385
HincII	GTYRAC	1 cut(s)	423
HindII	GTYRAC	1 cut(s)	423
HinfI	GANTC	2 cut(s)	199, 498
HpaI	GTTAAC	1 cut(s)	423
HpaII	CCGG	3 cut(s)	432, 670, 736
HphI	GGTGA	4 cut(s)	46, 70, 98, 151
Hpy166II	GTNNAC	4 cut(s)	340, 423, 445, 741
Hpy188I	TCNGA	2 cut(s)	73, 412
Hpy8I	GTNNAC	4 cut(s)	340, 423, 445, 741
Hpy99I	CGWCG	1 cut(s)	308
HpyAV	CCTTC	2 cut(s)	526, 805
HpyCH4III	ACNGT	1 cut(s)	427
HpyF10VI	GCNNNNNNNGC	6 cut(s)	23, 44, 177, 365, 560, 670
HpyF3I	CTNAG	4 cut(s)	70, 395, 707, 822
Hsp92I	GRCGYC	1 cut(s)	306
Hsp92II	CATG	3 cut(s)	37, 421, 732
HspAI	GCGC	1 cut(s)	385
KroI	GCCGGC	1 cut(s)	669
KroNI	GCCGGC	1 cut(s)	671
KspAI	GTTAAC	1 cut(s)	423
KspI	CCGCGG	1 cut(s)	281
Kzo9I	GATC	2 cut(s)	73, 771
LmnI	GCTCC	2 cut(s)	321, 606
Lsp1109I	GCAGC	5 cut(s)	25, 29, 564, 648, 719
LweI	GCATC	1 cut(s)	563
MalI	GATC	2 cut(s)	75, 773
MboI	GATC	2 cut(s)	73, 771
MboII	GAAGA	3 cut(s)	17, 142, 724
MflI	RGATCY	2 cut(s)	73, 771
MlsI	TGGCCA	1 cut(s)	47
MluCI	AATT	2 cut(s)	390, 815
MluNI	TGGCCA	1 cut(s)	47
MlyI	GAGTC	1 cut(s)	193
MmeI	TCCRAC	3 cut(s)	280, 435, 799
Mox20I	TGGCCA	1 cut(s)	47
MroNI	GCCGGC	1 cut(s)	669
MscI	TGGCCA	1 cut(s)	47
MseI	TTAA	2 cut(s)	422, 798
MslI	CAYNNNNRTG	1 cut(s)	648
Msp20I	TGGCCA	1 cut(s)	47
MspA1I	CMGCKG	1 cut(s)	280
MspI	CCGG	3 cut(s)	432, 670, 736
MvnI	CGCG	2 cut(s)	280, 387
MwoI	GCNNNNNNNGC	6 cut(s)	23, 44, 177, 365, 560, 670
NaeI	GCCGGC	1 cut(s)	671
NdeII	GATC	2 cut(s)	73, 771
NgoMIV	GCCGGC	1 cut(s)	669
NlaIII	CATG	3 cut(s)	37, 421, 732
NspI	RCATGY	1 cut(s)	421
PciI	ACATGT	1 cut(s)	417
PdiI	GCCGGC	1 cut(s)	671
PfeI	GAWTC	1 cut(s)	498
PflFI	GACNNNGTC	1 cut(s)	308
PkrI	GCNGC	8 cut(s)	19, 40, 279, 282, 553, 579, 663, 734
PleI	GAGTC	1 cut(s)	193
PpsI	GAGTC	1 cut(s)	193
PscI	ACATGT	1 cut(s)	417
PsuI	RGATCY	2 cut(s)	73, 771
PsyI	GACNNNGTC	1 cut(s)	308
RsaI	GTAC	2 cut(s)	339, 407
RsaNI	GTAC	2 cut(s)	338, 406
RseI	CAYNNNNRTG	1 cut(s)	648
SacII	CCGCGG	1 cut(s)	281
SaqAI	TTAA	2 cut(s)	422, 798
SatI	GCNGC	8 cut(s)	18, 39, 278, 281, 552, 578, 662, 733
Sau3AI	GATC	2 cut(s)	73, 771
SchI	GAGTC	1 cut(s)	193
SetI	ASST	7 cut(s)	222, 243, 303, 480, 491, 663, 823
SfaNI	GCATC	1 cut(s)	563
Sfr303I	CCGCGG	1 cut(s)	281
SgrBI	CCGCGG	1 cut(s)	281
SmiMI	CAYNNNNRTG	1 cut(s)	648
SmlI	CTYRAG	2 cut(s)	354, 493
SmoI	CTYRAG	2 cut(s)	354, 493
Sse9I	AATT	2 cut(s)	390, 815
TaaI	ACNGT	1 cut(s)	427
TaqI	TCGA	2 cut(s)	303, 363
TasI	AATT	2 cut(s)	390, 815
TatI	WGTACW	2 cut(s)	337, 405
TauI	GCSGC	3 cut(s)	280, 283, 554
TfiI	GAWTC	1 cut(s)	498
Tru1I	TTAA	2 cut(s)	422, 798
Tru9I	TTAA	2 cut(s)	422, 798
TscAI	CASTG	1 cut(s)	591
TseI	GCWGC	5 cut(s)	17, 38, 577, 661, 732
TspDTI	ATGAA	1 cut(s)	671
TspGWI	ACGGA	1 cut(s)	767
TspRI	CASTG	1 cut(s)	591
Tth111I	GACNNNGTC	1 cut(s)	308
XapI	RAATTY	1 cut(s)	390
XceI	RCATGY	1 cut(s)	421
XcmI	CCANNNNNNNNNTGG	1 cut(s)	378
XmiI	GTMKAC	1 cut(s)	444

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.