AT5G04250

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005498
Physical Location & Seq
Forward (+)
1176005 .. 1179008
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04250.1

Sequence Viewer

Length: 1038 bp
ATGGGGTATGAGCCTGATCCAGATGCTCTTAGGTGGGGTCTTCATGATCTGGAGGTTTGTACACTTACAAATGCTGGTTCTTGCTCTAGTGTAACAAGGTATGAGTCTGGTGGTGGTGGTACTCAAGGCTATGTTAGAGAAGGTTACAATCAGCCTGTGACTGGTTATGTTGACAATGATGCGGTGATTGCTCAGTTTTATCAAGACGAATTGTCTAGAGTTGCTCGAGCCGAAGCATCGGGGATCAATAGTCTTAGTCCTACTTCTGTTGTTGCACAAGACTGGCCTCATCCTCATCAAGGACAGGAAAATCAAGGGGAAGCCATTGATATTACTCAGGAAAGTGATATTCTTCATAATCATAACGGTAACATGGAAGATAAGAATGTTGCAAGGATAAGATTTGAAGGAGGGCAAAGTTCCCCATCTCGTGATGATGACTCAGTTTGTTCTGTAGAAATAGAAGAAGAATCTTGGTCAGAGGTTGGGAAGCGACTGAACCAAATGATACCTATTGCTCATGTGCCGAAAATCAATGGGGAATTACCGTCAGAGGATGAGCAGATTTCTGATCATGAACGGCTATTTCAGAGATTACAGCTTTATGGCTTGGTGGAGAACAAGATTGAAGGAGATGGAAACTGCCAGTTTCGGTCACTATCAGACCAGCTTTACCGCTCTCCAGAACATCATAATTTTGTCAGAGAACAAGTTGTTAATCAGCTTGCATATAATCGAGAGATATATGAAGGTTATGTTCCAATGGCGTACAATGACTATCTTAAAGCAATGAAACGGAATGGAGAATGGGGTGATCACGTTACTCTTCAGGCTGCTGCGGATTTGTTTGGTGTTCGGATGTTTGTGATAACTTCATTCAAGGATACATGCTACATTGAGATCTTGCCTCATTTTCAGAAGTCTAATCGCCTTATATGTTTAAGCTTCTGGGCTGAGGTTCATTACAATTCAATCTACCCTGAAGGAGAGCTGCCGATACCAGAAGGCAAGAAGAAGAAGAAATATTGGGTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

345

Amino Acids

39.23

Weight (kDa)

4.9

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 211 - 297 1.7e-11 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 678
AciI CCGC 3 cut(s) 182, 676, 839
AclWI GGATC 2 cut(s) 11, 251
AcuI CTGAAG 2 cut(s) 812, 1002
AfaI GTAC 3 cut(s) 61, 121, 770
AfiI CCNNNNNNNGG 2 cut(s) 161, 299
AgsI TTSAA 4 cut(s) 407, 629, 880, 972
AhdI GACNNNNNGTC 1 cut(s) 211
AluBI AGCT 5 cut(s) 601, 670, 724, 945, 991
AluI AGCT 5 cut(s) 601, 670, 724, 945, 991
AlwI GGATC 2 cut(s) 11, 251
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 1 cut(s) 284
ApeKI GCWGC 3 cut(s) 833, 836, 991
AsuHPI GGTGA 2 cut(s) 196, 824
AvaI CYCGRG 1 cut(s) 225
BauI CACGAG 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 3 cut(s) 820, 823, 978
BccI CCATC 2 cut(s) 433, 629
BceAI ACGGC 1 cut(s) 596
BciVI GTATCC 1 cut(s) 877
BclI TGATCA 2 cut(s) 571, 814
BfaI CTAG 2 cut(s) 87, 216
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 877
BglII AGATCT 1 cut(s) 900
BisI GCNGC 3 cut(s) 834, 837, 992
BlsI GCNGC 3 cut(s) 835, 838, 993
BmeRI GACNNNNNGTC 1 cut(s) 211
BmeT110I CYCGRG 1 cut(s) 225
BmsI GCATC 3 cut(s) 13, 169, 245
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 71, 666
Bpu10I CCTNAGC 1 cut(s) 954
BpuEI CTTGAG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 299
Bse1I ACTGG 3 cut(s) 166, 287, 646
Bse3DI GCAATG 1 cut(s) 795
BseGI GGATG 3 cut(s) 289, 562, 864
BseLI CCNNNNNNNGG 2 cut(s) 161, 299
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 4 cut(s) 206, 350, 456, 945
BseNI ACTGG 3 cut(s) 166, 287, 646
BseXI GCAGC 3 cut(s) 820, 823, 978
BshFI GGCC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 161, 299
BsnI GGCC 1 cut(s) 286
BsoBI CYCGRG 1 cut(s) 225
Bsp1407I TGTACA 1 cut(s) 59
Bsp143I GATC 6 cut(s) 16, 46, 243, 571, 814, 900
BspACI CCGC 3 cut(s) 182, 676, 839
BspANI GGCC 1 cut(s) 286
BspCNI CTCAG 4 cut(s) 205, 349, 455, 946
BspHI TCATGA 2 cut(s) 43, 574
BspPI GGATC 2 cut(s) 11, 251
BsrBI CCGCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 795
BsrGI TGTACA 1 cut(s) 59
BsrI ACTGG 3 cut(s) 166, 287, 646
BssMI GATC 6 cut(s) 16, 46, 243, 571, 814, 900
BssSI CACGAG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 368, 549
Bst6I CTCTTC 1 cut(s) 831
BstAUI TGTACA 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 726
BstDEI CTNAG 6 cut(s) 29, 192, 254, 336, 442, 954
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 3 cut(s) 289, 562, 864
BstKTI GATC 6 cut(s) 19, 49, 246, 574, 817, 903
BstMBI GATC 6 cut(s) 16, 46, 243, 571, 814, 900
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 891
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 3 cut(s) 820, 823, 978
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 900
BstYI RGATCY 1 cut(s) 900
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 1 cut(s) 286
BtsCI GGATG 3 cut(s) 289, 562, 864
Cac8I GCNNGC 1 cut(s) 726
CciI TCATGA 2 cut(s) 43, 574
Csp6I GTAC 3 cut(s) 60, 120, 769
CviAII CATG 5 cut(s) 44, 373, 521, 575, 888
CviQI GTAC 3 cut(s) 60, 120, 769
DdeI CTNAG 6 cut(s) 29, 192, 254, 336, 442, 954
DpnI GATC 6 cut(s) 18, 48, 245, 573, 816, 902
DpnII GATC 6 cut(s) 16, 46, 243, 571, 814, 900
DriI GACNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 831
Eam1105I GACNNNNNGTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 831
Eco57I CTGAAG 2 cut(s) 812, 1002
Eco88I CYCGRG 1 cut(s) 225
EcoNI CCTNNNNNAGG 1 cut(s) 297
FaeI CATG 5 cut(s) 47, 376, 524, 578, 891
FatI CATG 5 cut(s) 43, 372, 520, 574, 887
FbaI TGATCA 2 cut(s) 571, 814
Fnu4HI GCNGC 3 cut(s) 834, 837, 992
FokI GGATG 3 cut(s) 276, 569, 871
Fsp4HI GCNGC 3 cut(s) 834, 837, 992
FspBI CTAG 2 cut(s) 87, 216
GluI GCNGC 3 cut(s) 834, 837, 992
GsuI CTGGAG 2 cut(s) 71, 666
HaeIII GGCC 1 cut(s) 286
Hin1II CATG 5 cut(s) 47, 376, 524, 578, 891
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 3 cut(s) 104, 440, 470
HphI GGTGA 2 cut(s) 196, 824
Hpy166II GTNNAC 2 cut(s) 62, 172
Hpy188I TCNGA 9 cut(s) 481, 553, 571, 591, 664, 704, 858, 918, 1037
Hpy8I GTNNAC 2 cut(s) 62, 172
HpyAV CCTTC 6 cut(s) 134, 401, 623, 743, 977, 998
HpyCH4III ACNGT 2 cut(s) 368, 549
HpyCH4IV ACGT 1 cut(s) 819
HpyCH4V TGCA 3 cut(s) 275, 392, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 6 cut(s) 29, 192, 254, 336, 442, 954
HpySE526I ACGT 1 cut(s) 819
Hsp92II CATG 5 cut(s) 47, 376, 524, 578, 891
Ksp22I TGATCA 2 cut(s) 571, 814
Kzo9I GATC 6 cut(s) 16, 46, 243, 571, 814, 900
Lsp1109I GCAGC 3 cut(s) 820, 823, 978
LweI GCATC 3 cut(s) 13, 169, 245
MaeI CTAG 2 cut(s) 87, 216
MaeII ACGT 1 cut(s) 819
MaeIII GTNAC 6 cut(s) 91, 143, 157, 368, 654, 820
MalI GATC 6 cut(s) 18, 48, 245, 573, 816, 902
MbiI CCGCTC 1 cut(s) 678
MboI GATC 6 cut(s) 16, 46, 243, 571, 814, 900
MboII GAAGA 9 cut(s) 32, 344, 389, 476, 479, 818, 1024, 1027, 1030
MflI RGATCY 1 cut(s) 900
MluCI AATT 4 cut(s) 209, 542, 694, 967
MlyI GAGTC 2 cut(s) 113, 434
MnlI CCTC 8 cut(s) 46, 297, 303, 404, 475, 547, 918, 949
MseI TTAA 3 cut(s) 717, 783, 941
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 6 cut(s) 16, 46, 243, 571, 814, 900
NlaIII CATG 5 cut(s) 47, 376, 524, 578, 891
NmuCI GTSAC 2 cut(s) 157, 654
NspI RCATGY 1 cut(s) 891
PaeR7I CTCGAG 1 cut(s) 225
PagI TCATGA 2 cut(s) 43, 574
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 3 cut(s) 835, 838, 993
PleI GAGTC 2 cut(s) 112, 434
PpsI GAGTC 2 cut(s) 112, 434
PspXI VCTCGAGB 1 cut(s) 225
PsuI RGATCY 1 cut(s) 900
RsaI GTAC 3 cut(s) 61, 121, 770
RsaNI GTAC 3 cut(s) 60, 120, 769
SaqAI TTAA 3 cut(s) 717, 783, 941
SatI GCNGC 3 cut(s) 834, 837, 992
Sau3AI GATC 6 cut(s) 16, 46, 243, 571, 814, 900
SchI GAGTC 2 cut(s) 113, 434
SfaNI GCATC 3 cut(s) 13, 169, 245
SfcI CTRYAG 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 225
SlaI CTCGAG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 123, 225
SmoI CTYRAG 2 cut(s) 123, 225
Sse9I AATT 4 cut(s) 209, 542, 694, 967
SsiI CCGC 3 cut(s) 182, 676, 839
SspI AATATT 1 cut(s) 1025
SspMI CTAG 2 cut(s) 87, 216
TaaI ACNGT 2 cut(s) 368, 549
TaiI ACGT 1 cut(s) 822
TaqI TCGA 2 cut(s) 226, 736
TaqII GACCGA 1 cut(s) 642
TasI AATT 4 cut(s) 209, 542, 694, 967
TatI WGTACW 1 cut(s) 59
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 3 cut(s) 717, 783, 941
Tru9I TTAA 3 cut(s) 717, 783, 941
TseFI GTSAC 2 cut(s) 157, 654
TseI GCWGC 3 cut(s) 833, 836, 991
Tsp45I GTSAC 2 cut(s) 157, 654
TspDTI ATGAA 7 cut(s) 32, 344, 591, 762, 806, 864, 950
TspGWI ACGGA 1 cut(s) 811
XagI CCTNNNNNAGG 1 cut(s) 297
XbaI TCTAGA 1 cut(s) 215
XceI RCATGY 1 cut(s) 891
XhoI CTCGAG 1 cut(s) 225
XspI CTAG 2 cut(s) 87, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.