MD01G1105100.v1.1

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005498
Physical Location & Seq
Reverse (-)
21812366 .. 21816376
4011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1105100.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGTTAAACGAGCAGATGAAGACATATAACCTTGATCCCGACGTGCTTCGCTGGGGTCTCCATCTCTTGGATGTTTGCACATTGTCGAATGATAATTCTCATAGTACTGTTACGCGATACGACCAGGATTTTAGTCAAGTAGAGTATGTTAGAGAAGGTTATGTTGAGCCTTGTATTGTGGAGAATGATGAGATTATAGCTCATGCTTATCAAGAAGAGTTGTCGAGGTTCGCTTCTGTAGAAACATCTGGAGCTTCTGGTTATGGAGATGGGCAGTTGGAAGCGTCAATTCTTGAGCAGGATTGGCTTGGTCCATCAACTAGGCACAGTGGTTCTGGGCTTGAAAATGTTCAGGATGCAGTAAATGATTTCGGCAGAAATATTGACACCGATGAATATAGACATAATCAACATGGGGTGGATGATCCAAGGAGAAATGGAAATGAGGTAGCAACTGATCACTGCGAAGGAGAATACTCTATTAATGGGGAAGACTTTTTGCATTCACTGGACATAACAGATGAATTTTCTCTTGATGGTGAAGTAGGGAGAAGATTGAATCAGATGGCTTCTGTACCTCATGTTCCTAAAACGAATGAAAAATTACCCTCAGAGGATGAACAGTTATCAGATCATCAGAGGCTTTTGGATAGGCTGCAGTTGTATGATTTGGTGGAGTGTAAGGTCCAGGGAGATGGTAACTGTCAGTTTCGTGCTTTATCAGATCAACTATATCGTTCTCCTGAATACCATGGTGTTGTAAGAGACCAAATTATTCAACAGCTGATGAGTCACCCTGAAATGTACGAGGGTTACGTTCTTATGGGTTATGTTGATTATCTAAACAAGATGAGCAAGAATGGTGAATGGGGTGATCATGTCACATTGCAGGCTGCTGCAGATTCGTATGGTGTCAAGATATTTGTAATAACTTCTTTCCGAGATACATGTTACATTGAAATCCTTCCTCACGTTCAAAAGTCCAATAGAGTTATCTGCTTGAGCTTTTGGGCCGAAGTGCATTACAATTCGATTTATCCCGAAGGAGAACTTCCTCCGCCCAGCTTAAGGAAAAAGAAGAAATGGTGGAACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

366

Amino Acids

41.86

Weight (kDa)

4.7

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 230 - 319 4e-12 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 67
AccII CGCG 1 cut(s) 115
AciI CCGC 1 cut(s) 1058
AclWI GGATC 2 cut(s) 29, 419
AcsI RAATTY 1 cut(s) 524
AfaI GTAC 3 cut(s) 106, 576, 806
AfiI CCNNNNNNNGG 2 cut(s) 67, 1068
AflII CTTAAG 1 cut(s) 1066
AflIII ACRYGT 1 cut(s) 947
AgsI TTSAA 5 cut(s) 344, 559, 779, 959, 977
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 2 cut(s) 123, 687
AluBI AGCT 5 cut(s) 200, 254, 784, 1005, 1065
AluI AGCT 5 cut(s) 200, 254, 784, 1005, 1065
Alw26I GTCTC 2 cut(s) 62, 759
AlwI GGATC 2 cut(s) 29, 419
AoxI GGCC 1 cut(s) 1011
ApeKI GCWGC 3 cut(s) 655, 893, 896
ApoI RAATTY 1 cut(s) 524
AseI ATTAAT 1 cut(s) 483
Asp700I GAANNNNTTC 2 cut(s) 348, 963
AspS9I GGNCC 3 cut(s) 311, 685, 1011
AsuHPI GGTGA 4 cut(s) 551, 785, 875, 884
AvaII GGWCC 2 cut(s) 311, 685
BbsI GAAGAC 2 cut(s) 26, 498
BbvI GCAGC 3 cut(s) 642, 880, 883
BccI CCATC 6 cut(s) 69, 263, 322, 530, 559, 689
BciT130I CCWGG 2 cut(s) 125, 689
BclI TGATCA 2 cut(s) 457, 874
BcoDI GTCTC 2 cut(s) 62, 759
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 3 cut(s) 237, 656, 897
BfrI CTTAAG 1 cut(s) 1066
BisI GCNGC 3 cut(s) 656, 894, 897
BlsI GCNGC 3 cut(s) 657, 895, 898
BmcAI AGTACT 1 cut(s) 106
Bme1390I CCNGG 2 cut(s) 125, 689
Bme18I GGWCC 2 cut(s) 311, 685
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 3 cut(s) 311, 685, 1011
BmiI GGNNCC 1 cut(s) 1091
BmrFI CCNGG 2 cut(s) 125, 689
BmsI GCATC 1 cut(s) 346
BpiI GAAGAC 2 cut(s) 26, 498
BpmI CTGGAG 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 314, 1021
BsaI GGTCTC 2 cut(s) 62, 759
BsaJI CCNNGG 3 cut(s) 428, 688, 751
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 1068
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 884
BseBI CCWGG 2 cut(s) 125, 689
BseDI CCNNGG 3 cut(s) 428, 688, 751
BseGI GGATG 4 cut(s) 76, 361, 427, 622
BseLI CCNNNNNNNGG 2 cut(s) 67, 1068
BseMI GCAATG 1 cut(s) 884
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 1 cut(s) 513
BseXI GCAGC 3 cut(s) 642, 880, 883
BseYI CCCAGC 2 cut(s) 51, 1061
Bsh1236I CGCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 1013
BslI CCNNNNNNNGG 2 cut(s) 67, 1068
BsmAI GTCTC 2 cut(s) 62, 759
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 1 cut(s) 1013
Bso31I GGTCTC 2 cut(s) 62, 759
Bsp143I GATC 6 cut(s) 34, 424, 457, 631, 724, 874
Bsp19I CCATGG 1 cut(s) 751
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 1 cut(s) 1013
BspCNI CTCAG 1 cut(s) 623
BspFNI CGCG 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 1091
BspMAI CTGCAG 2 cut(s) 660, 901
BspPI GGATC 2 cut(s) 29, 419
BspTI CTTAAG 1 cut(s) 1066
BspTNI GGTCTC 2 cut(s) 62, 759
BsrDI GCAATG 1 cut(s) 884
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 3 cut(s) 428, 688, 751
BssMI GATC 6 cut(s) 34, 424, 457, 631, 724, 874
BssT1I CCWWGG 2 cut(s) 428, 751
Bst2UI CCWGG 2 cut(s) 125, 689
Bst4CI ACNGT 4 cut(s) 109, 329, 624, 704
Bst6I CTCTTC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 1066
BstC8I GCNNGC 1 cut(s) 891
BstDEI CTNAG 1 cut(s) 610
BstDSI CCRYGG 1 cut(s) 751
BstF5I GGATG 4 cut(s) 76, 361, 427, 622
BstFNI CGCG 1 cut(s) 115
BstKTI GATC 6 cut(s) 37, 427, 460, 634, 727, 877
BstMAI GTCTC 2 cut(s) 62, 759
BstMBI GATC 6 cut(s) 34, 424, 457, 631, 724, 874
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstNI CCWGG 2 cut(s) 125, 689
BstNSI RCATGY 1 cut(s) 951
BstSCI CCNGG 2 cut(s) 123, 687
BstSFI CTRYAG 3 cut(s) 237, 656, 897
BstUI CGCG 1 cut(s) 115
BstV1I GCAGC 3 cut(s) 642, 880, 883
BstV2I GAAGAC 2 cut(s) 26, 498
BstXI CCANNNNNNTGG 1 cut(s) 695
BsuRI GGCC 1 cut(s) 1013
BtgI CCRYGG 1 cut(s) 751
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 4 cut(s) 76, 361, 427, 622
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 3 cut(s) 334, 460, 506
Cac8I GCNNGC 1 cut(s) 891
Cfr13I GGNCC 3 cut(s) 311, 685, 1011
CseI GACGC 1 cut(s) 273
Csp6I GTAC 3 cut(s) 105, 575, 805
CviAII CATG 6 cut(s) 203, 413, 581, 752, 878, 948
CviQI GTAC 3 cut(s) 105, 575, 805
DdeI CTNAG 1 cut(s) 610
DpnI GATC 6 cut(s) 36, 426, 459, 633, 726, 876
DpnII GATC 6 cut(s) 34, 424, 457, 631, 724, 874
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 1047
Eco130I CCWWGG 2 cut(s) 428, 751
Eco31I GGTCTC 2 cut(s) 62, 759
Eco47I GGWCC 2 cut(s) 311, 685
EcoRII CCWGG 2 cut(s) 123, 687
EcoT14I CCWWGG 2 cut(s) 428, 751
ErhI CCWWGG 2 cut(s) 428, 751
FaeI CATG 6 cut(s) 206, 416, 584, 755, 881, 951
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FatI CATG 6 cut(s) 202, 412, 580, 751, 877, 947
FbaI TGATCA 2 cut(s) 457, 874
Fnu4HI GCNGC 3 cut(s) 656, 894, 897
FokI GGATG 4 cut(s) 83, 368, 434, 629
Fsp4HI GCNGC 3 cut(s) 656, 894, 897
FspBI CTAG 1 cut(s) 321
GluI GCNGC 3 cut(s) 656, 894, 897
GsaI CCCAGC 2 cut(s) 55, 1065
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 1 cut(s) 1013
HgaI GACGC 1 cut(s) 273
Hin1II CATG 6 cut(s) 206, 416, 584, 755, 881, 951
HinfI GANTC 3 cut(s) 559, 790, 902
HphI GGTGA 4 cut(s) 551, 785, 875, 884
Hpy188I TCNGA 6 cut(s) 564, 613, 631, 639, 724, 941
Hpy188III TCNNGA 9 cut(s) 38, 212, 249, 293, 353, 533, 743, 916, 1040
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 4 cut(s) 149, 461, 974, 1037
HpyCH4III ACNGT 4 cut(s) 109, 329, 624, 704
HpyCH4IV ACGT 3 cut(s) 42, 816, 972
HpyCH4V TGCA 7 cut(s) 78, 359, 502, 658, 889, 899, 1021
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 1 cut(s) 610
HpySE526I ACGT 3 cut(s) 42, 816, 972
Hsp92II CATG 6 cut(s) 206, 416, 584, 755, 881, 951
Ksp22I TGATCA 2 cut(s) 457, 874
Kzo9I GATC 6 cut(s) 34, 424, 457, 631, 724, 874
LmnI GCTCC 1 cut(s) 251
Lsp1109I GCAGC 3 cut(s) 642, 880, 883
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 321
MaeII ACGT 3 cut(s) 42, 816, 972
MaeIII GTNAC 6 cut(s) 109, 698, 791, 812, 880, 950
MalI GATC 6 cut(s) 36, 426, 459, 633, 726, 876
MboI GATC 6 cut(s) 34, 424, 457, 631, 724, 874
MboII GAAGA 5 cut(s) 31, 227, 503, 564, 1090
MluCI AATT 6 cut(s) 94, 288, 524, 602, 771, 1027
MlyI GAGTC 1 cut(s) 799
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 9 cut(s) 219, 439, 588, 607, 619, 633, 802, 978, 1065
MroXI GAANNNNTTC 2 cut(s) 348, 963
MseI TTAA 3 cut(s) 5, 483, 1067
MspA1I CMGCKG 1 cut(s) 784
MspCI CTTAAG 1 cut(s) 1066
MspR9I CCNGG 2 cut(s) 125, 689
Mva1269I GAATGC 1 cut(s) 502
MvaI CCWGG 2 cut(s) 125, 689
MvnI CGCG 1 cut(s) 115
MwoI GCNNNNNNNGC 1 cut(s) 304
NcoI CCATGG 1 cut(s) 751
NdeII GATC 6 cut(s) 34, 424, 457, 631, 724, 874
NlaIII CATG 6 cut(s) 206, 416, 584, 755, 881, 951
NlaIV GGNNCC 1 cut(s) 1091
NmuCI GTSAC 2 cut(s) 791, 880
NspI RCATGY 1 cut(s) 951
PciI ACATGT 1 cut(s) 947
PcsI WCGNNNNNNNCGW 1 cut(s) 813
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 2 cut(s) 348, 963
PfeI GAWTC 2 cut(s) 559, 902
PflMI CCANNNNNTGG 1 cut(s) 67
PkrI GCNGC 3 cut(s) 657, 895, 898
PleI GAGTC 1 cut(s) 798
PpsI GAGTC 1 cut(s) 798
PscI ACATGT 1 cut(s) 947
PshBI ATTAAT 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 123, 687
PspFI CCCAGC 2 cut(s) 51, 1061
PspGI CCWGG 2 cut(s) 123, 687
PspN4I GGNNCC 1 cut(s) 1091
PspPI GGNCC 3 cut(s) 311, 685, 1011
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PstI CTGCAG 2 cut(s) 660, 901
PvuII CAGCTG 1 cut(s) 784
RsaI GTAC 3 cut(s) 106, 576, 806
RsaNI GTAC 3 cut(s) 105, 575, 805
SaqAI TTAA 3 cut(s) 5, 483, 1067
SatI GCNGC 3 cut(s) 656, 894, 897
Sau3AI GATC 6 cut(s) 34, 424, 457, 631, 724, 874
Sau96I GGNCC 3 cut(s) 311, 685, 1011
ScaI AGTACT 1 cut(s) 106
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 2 cut(s) 125, 689
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 3 cut(s) 237, 656, 897
SinI GGWCC 2 cut(s) 311, 685
SmlI CTYRAG 3 cut(s) 293, 1000, 1066
SmoI CTYRAG 3 cut(s) 293, 1000, 1066
Sse9I AATT 6 cut(s) 94, 288, 524, 602, 771, 1027
SsiI CCGC 1 cut(s) 1058
SspI AATATT 1 cut(s) 382
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 123, 687
StyI CCWWGG 2 cut(s) 428, 751
TaaI ACNGT 4 cut(s) 109, 329, 624, 704
TaiI ACGT 3 cut(s) 45, 819, 975
TaqI TCGA 3 cut(s) 86, 224, 1031
TasI AATT 6 cut(s) 94, 288, 524, 602, 771, 1027
TatI WGTACW 1 cut(s) 104
TfiI GAWTC 2 cut(s) 559, 902
Tru1I TTAA 3 cut(s) 5, 483, 1067
Tru9I TTAA 3 cut(s) 5, 483, 1067
TscAI CASTG 3 cut(s) 334, 467, 513
TseFI GTSAC 2 cut(s) 791, 880
TseI GCWGC 3 cut(s) 655, 893, 896
Tsp45I GTSAC 2 cut(s) 791, 880
TspDTI ATGAA 5 cut(s) 32, 408, 537, 612, 633
TspRI CASTG 3 cut(s) 334, 467, 513
Van91I CCANNNNNTGG 1 cut(s) 67
Vha464I CTTAAG 1 cut(s) 1066
VpaK11BI GGWCC 2 cut(s) 311, 685
VspI ATTAAT 1 cut(s) 483
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 951
XmnI GAANNNNTTC 2 cut(s) 348, 963
XspI CTAG 1 cut(s) 321
ZrmI AGTACT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.