Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Forward (+)

1221448 .. 1228841

Gene Structure 7394 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G04360.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGCACTGACACTGACACCAACAAGTTCCGTTCATCTTCTCAGTTCCATATCCGTCGCGCGTCCTCGGATCTTCGCCGCTGATTTTAATCTTCGATCGCGGTGGCGACGTCGTCGGCCTGTTACGTCAATTTCTAACTTCCGTCTCAGACTTCCTTCGAAAACTTCGCTTCACTGTCTCTGTTCTTCGTCTTCCGCTTCATCTCCTATGTCGCTCGAAGTCTCCAGTCCCAATTCTCAGTTTCTTGATTGTTTGATCTATTCAAGAGCGTATTGGGTTACCCAAGGCGTGATTGCATGGAATGTGGATGTTGGTGAAGGGTCTTGTTACTTTTACGCGAGCAAAAGTGCTGGTTTATCATTCAGCGAAGATGGAATCGATGGTTATGATTTAAGAATCAAGCTTGAAGCTGAATCCGGTTCACTTCCTGCAGATGTAATTGAGAAGTTTCCACATATTAGGAATTACAAATCGTTCAAAGTTCCTAAGGATTTGGATATCAGGGATCTTGTCAAAAGCCAGTTAGCTGTTGTTTGTTTTGATGCGGAAGGACGGCTGATTCAAGGAACTGGGTTGCAGTTGCCTGGCGTCCTGGATGAATTGTTCTCATATGATGGTCCCCTAGGTGCACATTTTACACCAGAAGGTGTCTCTCTTCACCTCTGGGCTCCCACTGCTCAAGCAGTATCTGTTTGCATCTATAAAAACCCACTTGACAAGAGTCCGATGGAAATTTGCCCACTCAAAGAGGCTAACGGTGTGTGGAGCACTGAAGGAGCTTGTAGTTGGGGAGGATGCTATTACGTGTATAAAGTATCAGTCTATCATCCAAGCACCATGAAATTGGAAACTTGCTATGCTAATGATCCTTATGCACGGGGGCTTTCAGCAGATGGGAGGAAAACTTTTCTGGTTAATCTTGATTCTGATGATCTAAAACCTGAAGGATGGGACAACTTAGCAGATAAGAAACCATGCCTAAGATCCTTCTCAGATATAAGTATTTATGAGCTGCATGTGAGGGATTTCAGTGCCAATGATGAGACTGTTGAGCCTGAAAATCGTGGTGGATATCTGGCTTTCACTTCAAAGGATTCTGCAGGTGTGAAACATCTTCAGAAATTAGTTGATGCAGGTCTTACCCATCTACATCTTCTTCCAACATTTCAGTTTGGTGATGTTGATGATGAGAAGGAGAATTGGAAGTCTGTAGACACCAGTCTGCTGGAAGGATTACGACCTGATTCAACTGAGGCACAAGCTCGTATTACAGAAATCCAGAATGATGATGGCTATAACTGGGGGTATAATCCTGTGCTCTGGGGAGTCCCAAAAGGAAGCTATGCTAGTGATCCGACTGGTCCATGCCGCATAATTGAATTTAGAAAAATGGTTCAGGCTCTTAATTGTACTGGTCTTAATGTCGTCTTAGATGTTGTTTACAACCACTTGCATGCAAGTGGGCCACATGACAAAGAATCTGTTCTTGATAAGATAGTTCCAGGTTACTATTTGAGAAGGAACAGTGATGGTTTTATTGAAAACAGTACATGCGTAAACAACACTGCTAGCGAGCACTATATGGTTGATCGTCTCATACGGGATGATCTATTAAATTGGGTTGTTAACTATAAGGTTGATGGTTTCCGTTTTGATCTTATGGGTCATATAATGAAAGCTACAATTGTAAACGCAAAATCTGCAATTGGTAGCTTGAGAAAGGAAACAGATGGAGTTGATGGTTCAAGAATTTATTTATATGGCGAAGGATGGAACTTTGGTGAAGTTGCTGAAAATGGACGTGGAATAAATGCGTCACAGTTCAATTTAGGCGGGACAGGAATTGGAAGTTTTAACGACCGTATACGAGATGCAACTCTTGGTGGATCTCCATTTGGTCATCCTCTTCAACAAGGATTCATTACAGGTTTATTGTTACAGCCTAACGCTCATGACCATGGTTCAGAAGCTACACAGGAACTGATGCTTTCCACTGCGAAAAATCACATCCAGACTGGTATGGCAGCAAATTTGAAGGATTATATGCTGACCAACCACGAAGGAAAGGAGGTGAAGGGATCAGAGGTTTTAATGCATGATGCTACACCTGTTGCATATGCTTCACTGCCAACAGAAACTATTAATTACGTATCTGCTCATGACAATGAAACTCTCTTTGACATTATCAGTTTGAAGACACCGATGGAAATATCTGTTGATGAAAGGTGTCGAATCAATCATTTGGCGTCAAGTATGATTGCCCTCTCTCAGGGTATTCCATTCTTCCATGCTGGGGATGAGATTCTACGCTCGAAATCACTTGATCGAGATTCTTACAACTCCGGTGACTGGTTCAATAGGCTGGACTTCAGCTACAGTTCCAACAATTGGGGTGTTGGGCTTCCTCCAAAAGGGAAAAACGAACACAACTGGCCACTGATCAAACCGAGGCTACAAGATCCATCTTTCAAGCCTAAGAGCAGTCACATAGTTGCCACCCTCCACAATTTCTTAGATTTGTTACGCATCAGATACTCTTCACCTCTCTTTCGTTTAGATACAGCAAGGGCTATACAGGAGCGAGTGCGATTTCATAACACAGGCCCCTCATCAATCCCTGGTGCCATTGTCATGAGCATTGAAGATGGTCACAGAGGCATCCCAAGTGTATCTCAGATCGATCCAATCTACTCACTCATTGTGGTAATCTTCAATGCACGCCCGTCTGAGTTCTCATATCCCAGTCCCGCTCTAAAAGACAGGAAGCTCGAATTACACCCGGTTCAGGTGATGTCAGCCGATGAAATAGTCAAGAAATCAGTTTACGACTCGTTTTCTGGGGGCTTCACTGTACCGGCAAGAACAACGACGGTGTTTGTTGAATCGCGGAATGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

965

Amino Acids

107.07

Weight (kDa)

5.94

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	88 - 200	7.3e-33	Pullulanase N2 domain
CBM_48	PF02922	207 - 292	1.9e-09	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	792 - 961	8.2e-63	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1091
AatII	GACGTC	1 cut(s)	112
Acc36I	ACCTGC	2 cut(s)	1091, 1124
AccB1I	GGYRCC	1 cut(s)	2621
AccB7I	CCANNNNNTGG	1 cut(s)	2388
AccBSI	CCGCTC	1 cut(s)	2750
AccI	GTMKAC	2 cut(s)	1212, 1864
AccII	CGCG	5 cut(s)	59, 61, 100, 338, 2887
AciI	CCGC	8 cut(s)	78, 100, 195, 545, 1369, 1833, 2748, 2887
AclWI	GGATC	9 cut(s)	77, 513, 860, 978, 1346, 1894, 2086, 2453, 2675
AcoI	YGGCCR	1 cut(s)	2432
AcsI	RAATTY	4 cut(s)	732, 1379, 1749, 2029
AcuI	CTGAAG	4 cut(s)	792, 963, 1100, 2353
AcyI	GRCGYC	3 cut(s)	109, 588, 2246
AfaI	GTAC	3 cut(s)	1411, 1549, 2853
AfiI	CCNNNNNNNGG	6 cut(s)	2269, 2293, 2349, 2388, 2411, 2785
AflIII	ACRYGT	1 cut(s)	804
AjiI	CACGTC	1 cut(s)	1802
AjnI	CCWGG	4 cut(s)	583, 591, 1501, 2617
AloI	GAACNNNNNNTCC	2 cut(s)	587, 619
Alw21I	GWGCWC	4 cut(s)	631, 770, 1320, 1578
Alw26I	GTCTC	6 cut(s)	149, 182, 226, 655, 1037, 1598
Alw44I	GTGCAC	1 cut(s)	627
AlwI	GGATC	9 cut(s)	77, 513, 860, 978, 1346, 1894, 2086, 2453, 2675
AlwNI	CAGNNNCTG	2 cut(s)	689, 1981
AoxI	GGCC	4 cut(s)	116, 1463, 2432, 2602
ApaLI	GTGCAC	1 cut(s)	627
ApeKI	GCWGC	2 cut(s)	1012, 2024
ApoI	RAATTY	4 cut(s)	732, 1379, 1749, 2029
AseI	ATTAAT	1 cut(s)	2142
Asp700I	GAANNNNTTC	1 cut(s)	1482
AspA2I	CCTAGG	1 cut(s)	622
AspLEI	GCGC	1 cut(s)	61
AspS9I	GGNCC	4 cut(s)	617, 1361, 1463, 2603
AsuC2I	CCSGG	1 cut(s)	2780
AsuHPI	GGTGA	8 cut(s)	326, 650, 1187, 1793, 2083, 2357, 2532, 2800
AsuII	TTCGAA	1 cut(s)	158
AsuNHI	GCTAGC	1 cut(s)	1568
AvaII	GGWCC	2 cut(s)	617, 1361
AvrII	CCTAGG	1 cut(s)	622
AxyI	CCTNAGG	1 cut(s)	486
BaeGI	GKGCMC	1 cut(s)	631
BalI	TGGCCA	1 cut(s)	2434
BanI	GGYRCC	1 cut(s)	2621
BanII	GRGCYC	1 cut(s)	670
BbsI	GAAGAC	2 cut(s)	183, 2201
Bbv12I	GWGCWC	4 cut(s)	631, 770, 1320, 1578
BbvI	GCAGC	2 cut(s)	999, 2036
BceAI	ACGGC	1 cut(s)	569
BciT130I	CCWGG	4 cut(s)	585, 593, 1503, 2619
BclI	TGATCA	1 cut(s)	2439
BcnI	CCSGG	1 cut(s)	2780
BcoDI	GTCTC	6 cut(s)	149, 182, 226, 655, 1037, 1598
BfaI	CTAG	3 cut(s)	623, 1347, 1569
BfmI	CTRYAG	4 cut(s)	429, 1098, 1209, 2374
BfuAI	ACCTGC	2 cut(s)	1091, 1124
BisI	GCNGC	4 cut(s)	78, 1013, 1369, 2025
BlnI	CCTAGG	1 cut(s)	622
BlsI	GCNGC	4 cut(s)	79, 1014, 1370, 2026
Bme1390I	CCNGG	5 cut(s)	585, 593, 1503, 2619, 2780
Bme18I	GGWCC	2 cut(s)	617, 1361
BmgBI	CACGTC	1 cut(s)	1802
BmgT120I	GGNCC	4 cut(s)	617, 1361, 1463, 2603
BmiI	GGNNCC	4 cut(s)	619, 669, 2605, 2623
BmrFI	CCNGG	5 cut(s)	585, 593, 1503, 2619, 2780
BmrI	ACTGGG	3 cut(s)	579, 1309, 2736
BmsI	GCATC	9 cut(s)	532, 705, 785, 1120, 1861, 1974, 2089, 2535, 2667
BmtI	GCTAGC	1 cut(s)	1572
BmuI	ACTGGG	3 cut(s)	579, 1309, 2736
BoxI	GACNNNNGTC	2 cut(s)	720, 1218
BpiI	GAAGAC	2 cut(s)	183, 2201
BpmI	CTGGAG	1 cut(s)	208
Bpu14I	TTCGAA	1 cut(s)	158
BpuEI	CTTGAG	2 cut(s)	663, 1735
BpuMI	CCSGG	1 cut(s)	2780
Bsa29I	ATCGAT	2 cut(s)	378, 2679
BsaAI	YACGTR	2 cut(s)	805, 2149
BsaBI	GATNNNNATC	1 cut(s)	87
BsaHI	GRCGYC	3 cut(s)	109, 588, 2246
BsaJI	CCNNGG	6 cut(s)	65, 283, 622, 1957, 2447, 2617
BsaWI	WCCGGW	2 cut(s)	416, 2342
Bsc4I	CCNNNNNNNGG	6 cut(s)	2269, 2293, 2349, 2388, 2411, 2785
Bse118I	RCCGGY	1 cut(s)	2854
Bse21I	CCTNAGG	1 cut(s)	486
Bse8I	GATNNNNATC	1 cut(s)	87
BseBI	CCWGG	4 cut(s)	585, 593, 1503, 2619
BseCI	ATCGAT	2 cut(s)	378, 2679
BseDI	CCNNGG	6 cut(s)	65, 283, 622, 1957, 2447, 2617
BseJI	GATNNNNATC	1 cut(s)	87
BseLI	CCNNNNNNNGG	6 cut(s)	2269, 2293, 2349, 2388, 2411, 2785
BseMII	CTCAG	8 cut(s)	55, 160, 251, 1005, 1242, 2282, 2687, 2718
BseSI	GKGCMC	1 cut(s)	631
BseXI	GCAGC	2 cut(s)	999, 2036
BseYI	CCCAGC	1 cut(s)	2291
Bsh1236I	CGCG	5 cut(s)	59, 61, 100, 338, 2887
Bsh1285I	CGRYCG	2 cut(s)	98, 1861
BshFI	GGCC	4 cut(s)	118, 1465, 2434, 2604
BshNI	GGYRCC	1 cut(s)	2621
BshVI	ATCGAT	2 cut(s)	378, 2679
BsiEI	CGRYCG	2 cut(s)	98, 1861
BsiHKAI	GWGCWC	4 cut(s)	631, 770, 1320, 1578
BsiSI	CCGG	4 cut(s)	417, 2343, 2780, 2855
BslFI	GGGAC	6 cut(s)	213, 603, 965, 1313, 1849, 2730
BslI	CCNNNNNNNGG	6 cut(s)	2269, 2293, 2349, 2388, 2411, 2785
BsmAI	GTCTC	6 cut(s)	149, 182, 226, 655, 1037, 1598
BsmBI	CGTCTC	2 cut(s)	149, 1598
BsmFI	GGGAC	6 cut(s)	213, 603, 965, 1313, 1849, 2730
BsnI	GGCC	4 cut(s)	118, 1465, 2434, 2604
Bsp119I	TTCGAA	1 cut(s)	158
Bsp1286I	GDGCHC	5 cut(s)	631, 670, 770, 1320, 1578
Bsp19I	CCATGG	1 cut(s)	1957
BspACI	CCGC	8 cut(s)	78, 100, 195, 545, 1369, 1833, 2748, 2887
BspANI	GGCC	4 cut(s)	118, 1465, 2434, 2604
BspCNI	CTCAG	8 cut(s)	54, 159, 250, 1004, 1243, 2281, 2686, 2719
BspDI	ATCGAT	2 cut(s)	378, 2679
BspFNI	CGCG	5 cut(s)	59, 61, 100, 338, 2887
BspHI	TCATGA	3 cut(s)	1951, 2158, 2631
BspLI	GGNNCC	4 cut(s)	619, 669, 2605, 2623
BspMAI	CTGCAG	2 cut(s)	433, 1102
BspMI	ACCTGC	2 cut(s)	1091, 1124
BspOI	GCTAGC	1 cut(s)	1572
BspPI	GGATC	9 cut(s)	77, 513, 860, 978, 1346, 1894, 2086, 2453, 2675
BspT104I	TTCGAA	1 cut(s)	158
BspT107I	GGYRCC	1 cut(s)	2621
BsrBI	CCGCTC	1 cut(s)	2750
BsrFI	RCCGGY	1 cut(s)	2854
BssAI	RCCGGY	1 cut(s)	2854
BssECI	CCNNGG	6 cut(s)	65, 283, 622, 1957, 2447, 2617
BssNAI	GTATAC	1 cut(s)	1865
BssNI	GRCGYC	3 cut(s)	109, 588, 2246
BssT1I	CCWWGG	3 cut(s)	283, 622, 1957
Bst1107I	GTATAC	1 cut(s)	1865
Bst2UI	CCWGG	4 cut(s)	585, 593, 1503, 2619
Bst6I	CTCTTC	3 cut(s)	660, 1911, 2542
BstACI	GRCGYC	3 cut(s)	109, 588, 2246
BstAPI	GCANNNNNTGC	1 cut(s)	1700
BstBAI	YACGTR	2 cut(s)	805, 2149
BstBI	TTCGAA	1 cut(s)	158
BstC8I	GCNNGC	5 cut(s)	340, 1455, 1570, 1574, 2719
BstDSI	CCRYGG	1 cut(s)	1957
BstEII	GGTNACC	1 cut(s)	277
BstENI	CCTNNNNNAGG	2 cut(s)	2267, 2409
BstFNI	CGCG	5 cut(s)	59, 61, 100, 338, 2887
BstHHI	GCGC	1 cut(s)	61
BstMAI	GTCTC	6 cut(s)	149, 182, 226, 655, 1037, 1598
BstMCI	CGRYCG	2 cut(s)	98, 1861
BstMWI	GCNNNNNNNGC	2 cut(s)	674, 1700
BstNI	CCWGG	4 cut(s)	585, 593, 1503, 2619
BstNSI	RCATGY	3 cut(s)	1019, 1457, 1554
BstPAI	GACNNNNGTC	2 cut(s)	720, 1218
BstPI	GGTNACC	1 cut(s)	277
BstSCI	CCNGG	5 cut(s)	583, 591, 1501, 2617, 2778
BstSFI	CTRYAG	4 cut(s)	429, 1098, 1209, 2374
BstSLI	GKGCMC	1 cut(s)	631
BstSNI	TACGTA	1 cut(s)	2149
BstUI	CGCG	5 cut(s)	59, 61, 100, 338, 2887
BstV1I	GCAGC	2 cut(s)	999, 2036
BstV2I	GAAGAC	2 cut(s)	183, 2201
BstX2I	RGATCY	5 cut(s)	69, 505, 983, 1886, 2458
BstXI	CCANNNNNNTGG	2 cut(s)	844, 1225
BstYI	RGATCY	5 cut(s)	69, 505, 983, 1886, 2458
BstZ17I	GTATAC	1 cut(s)	1865
Bsu15I	ATCGAT	2 cut(s)	378, 2679
Bsu36I	CCTNAGG	1 cut(s)	486
BsuRI	GGCC	4 cut(s)	118, 1465, 2434, 2604
BsuTUI	ATCGAT	2 cut(s)	378, 2679
BtgI	CCRYGG	1 cut(s)	1957
BtrI	CACGTC	1 cut(s)	1802
BtsI	GCAGTG	4 cut(s)	672, 1563, 1992, 2123
BveI	ACCTGC	2 cut(s)	1091, 1124
Cac8I	GCNNGC	5 cut(s)	340, 1455, 1570, 1574, 2719
CaiI	CAGNNNCTG	2 cut(s)	689, 1981
CciI	TCATGA	3 cut(s)	1951, 2158, 2631
CfoI	GCGC	1 cut(s)	61
Cfr10I	RCCGGY	1 cut(s)	2854
Cfr13I	GGNCC	4 cut(s)	617, 1361, 1463, 2603
ClaI	ATCGAT	2 cut(s)	378, 2679
CseI	GACGC	4 cut(s)	50, 577, 1803, 2235
Csp6I	GTAC	3 cut(s)	1410, 1548, 2852
CviQI	GTAC	3 cut(s)	1410, 1548, 2852
EaeI	YGGCCR	1 cut(s)	2432
Eam1104I	CTCTTC	3 cut(s)	660, 1911, 2542
EarI	CTCTTC	3 cut(s)	660, 1911, 2542
Eco105I	TACGTA	1 cut(s)	2149
Eco130I	CCWWGG	3 cut(s)	283, 622, 1957
Eco24I	GRGCYC	1 cut(s)	670
Eco32I	GATATC	2 cut(s)	499, 1073
Eco47I	GGWCC	2 cut(s)	617, 1361
Eco57I	CTGAAG	4 cut(s)	792, 963, 1100, 2353
Eco81I	CCTNAGG	1 cut(s)	486
Eco91I	GGTNACC	1 cut(s)	277
EcoNI	CCTNNNNNAGG	2 cut(s)	2267, 2409
EcoO109I	RGGNCCY	1 cut(s)	2603
EcoO65I	GGTNACC	1 cut(s)	277
EcoRII	CCWGG	4 cut(s)	583, 591, 1501, 2617
EcoRV	GATATC	2 cut(s)	499, 1073
EcoT14I	CCWWGG	3 cut(s)	283, 622, 1957
EcoT22I	ATGCAT	1 cut(s)	2097
EcoT38I	GRGCYC	1 cut(s)	670
ErhI	CCWWGG	3 cut(s)	283, 622, 1957
Esp3I	CGTCTC	2 cut(s)	149, 1598
FaqI	GGGAC	6 cut(s)	213, 603, 965, 1313, 1849, 2730
FauI	CCCGC	2 cut(s)	1826, 2755
FauNDI	CATATG	2 cut(s)	610, 2116
FbaI	TGATCA	1 cut(s)	2439
FblI	GTMKAC	2 cut(s)	1212, 1864
Fnu4HI	GCNGC	4 cut(s)	78, 1013, 1369, 2025
FriOI	GRGCYC	1 cut(s)	670
Fsp4HI	GCNGC	4 cut(s)	78, 1013, 1369, 2025
FspBI	CTAG	3 cut(s)	623, 1347, 1569
GlaI	GCGC	1 cut(s)	60
GluI	GCNGC	4 cut(s)	78, 1013, 1369, 2025
GsaI	CCCAGC	1 cut(s)	2295
GsuI	CTGGAG	1 cut(s)	208
HaeIII	GGCC	4 cut(s)	118, 1465, 2434, 2604
HapII	CCGG	4 cut(s)	417, 2343, 2780, 2855
HgaI	GACGC	4 cut(s)	50, 577, 1803, 2235
HhaI	GCGC	1 cut(s)	61
Hin1I	GRCGYC	3 cut(s)	109, 588, 2246
Hin6I	GCGC	1 cut(s)	59
HinP1I	GCGC	1 cut(s)	59
HincII	GTYRAC	1 cut(s)	1627
HindII	GTYRAC	1 cut(s)	1627
HindIII	AAGCTT	1 cut(s)	401
HpaI	GTTAAC	1 cut(s)	1627
HpaII	CCGG	4 cut(s)	417, 2343, 2780, 2855
HphI	GGTGA	8 cut(s)	326, 650, 1187, 1793, 2083, 2357, 2532, 2800
Hpy166II	GTNNAC	9 cut(s)	422, 629, 1213, 1441, 1558, 1627, 1690, 1865, 2824
Hpy8I	GTNNAC	9 cut(s)	422, 629, 1213, 1441, 1558, 1627, 1690, 1865, 2824
Hpy99I	CGWCG	5 cut(s)	59, 111, 114, 117, 2872
HpyCH4IV	ACGT	5 cut(s)	109, 125, 804, 1801, 2148
HpyF10VI	GCNNNNNNNGC	2 cut(s)	674, 1700
HpySE526I	ACGT	5 cut(s)	109, 125, 804, 1801, 2148
Hsp92I	GRCGYC	3 cut(s)	109, 588, 2246
HspAI	GCGC	1 cut(s)	59
Ksp22I	TGATCA	1 cut(s)	2439
KspAI	GTTAAC	1 cut(s)	1627
LmnI	GCTCC	4 cut(s)	673, 765, 776, 2578
Lsp1109I	GCAGC	2 cut(s)	999, 2036
LweI	GCATC	9 cut(s)	532, 705, 785, 1120, 1861, 1974, 2089, 2535, 2667
MaeI	CTAG	3 cut(s)	623, 1347, 1569
MaeII	ACGT	5 cut(s)	109, 125, 804, 1801, 2148
MbiI	CCGCTC	1 cut(s)	2750
MfeI	CAATTG	3 cut(s)	1683, 1704, 2386
MflI	RGATCY	5 cut(s)	69, 505, 983, 1886, 2458
MhlI	GDGCHC	5 cut(s)	631, 670, 770, 1320, 1578
MlsI	TGGCCA	1 cut(s)	2434
MluNI	TGGCCA	1 cut(s)	2434
MlyI	GAGTC	3 cut(s)	730, 1335, 2822
MmeI	TCCRAC	3 cut(s)	1184, 1379, 2406
Mox20I	TGGCCA	1 cut(s)	2434
Mph1103I	ATGCAT	1 cut(s)	2097
MroXI	GAANNNNTTC	1 cut(s)	1482
MscI	TGGCCA	1 cut(s)	2434
MslI	CAYNNNNRTG	5 cut(s)	1452, 1458, 1956, 2163, 2630
Msp20I	TGGCCA	1 cut(s)	2434
MspA1I	CMGCKG	1 cut(s)	80
MspI	CCGG	4 cut(s)	417, 2343, 2780, 2855
MspR9I	CCNGG	5 cut(s)	585, 593, 1503, 2619, 2780
MunI	CAATTG	3 cut(s)	1683, 1704, 2386
MvaI	CCWGG	4 cut(s)	585, 593, 1503, 2619
MvnI	CGCG	5 cut(s)	59, 61, 100, 338, 2887
MwoI	GCNNNNNNNGC	2 cut(s)	674, 1700
NciI	CCSGG	1 cut(s)	2780
NcoI	CCATGG	1 cut(s)	1957
NdeI	CATATG	2 cut(s)	610, 2116
NheI	GCTAGC	1 cut(s)	1568
NlaIV	GGNNCC	4 cut(s)	619, 669, 2605, 2623
NmuCI	GTSAC	4 cut(s)	1815, 2345, 2483, 2648
NsiI	ATGCAT	1 cut(s)	2097
NspI	RCATGY	3 cut(s)	1019, 1457, 1554
NspV	TTCGAA	1 cut(s)	158
PaeI	GCATGC	1 cut(s)	1457
PagI	TCATGA	3 cut(s)	1951, 2158, 2631
PaqCI	CACCTGC	1 cut(s)	1091
PdmI	GAANNNNTTC	1 cut(s)	1482
PflFI	GACNNNGTC	1 cut(s)	111
PflMI	CCANNNNNTGG	1 cut(s)	2388
PfoI	TCCNGGA	1 cut(s)	591
PkrI	GCNGC	4 cut(s)	79, 1014, 1370, 2026
Ple19I	CGATCG	1 cut(s)	98
PleI	GAGTC	3 cut(s)	729, 1334, 2822
PpsI	GAGTC	3 cut(s)	729, 1334, 2822
Ppu21I	YACGTR	2 cut(s)	805, 2149
PshAI	GACNNNNGTC	2 cut(s)	720, 1218
PshBI	ATTAAT	1 cut(s)	2142
Psp6I	CCWGG	4 cut(s)	583, 591, 1501, 2617
PspEI	GGTNACC	1 cut(s)	277
PspFI	CCCAGC	1 cut(s)	2291
PspGI	CCWGG	4 cut(s)	583, 591, 1501, 2617
PspN4I	GGNNCC	4 cut(s)	619, 669, 2605, 2623
PspPI	GGNCC	4 cut(s)	617, 1361, 1463, 2603
PstI	CTGCAG	2 cut(s)	433, 1102
PstNI	CAGNNNCTG	2 cut(s)	689, 1981
PsuI	RGATCY	5 cut(s)	69, 505, 983, 1886, 2458
PsyI	GACNNNGTC	1 cut(s)	111
PvuI	CGATCG	1 cut(s)	98
RsaI	GTAC	3 cut(s)	1411, 1549, 2853
RsaNI	GTAC	3 cut(s)	1410, 1548, 2852
RseI	CAYNNNNRTG	5 cut(s)	1452, 1458, 1956, 2163, 2630
SatI	GCNGC	4 cut(s)	78, 1013, 1369, 2025
Sau96I	GGNCC	4 cut(s)	617, 1361, 1463, 2603
SchI	GAGTC	3 cut(s)	730, 1335, 2822
ScrFI	CCNGG	5 cut(s)	585, 593, 1503, 2619, 2780
SduI	GDGCHC	5 cut(s)	631, 670, 770, 1320, 1578
SfaNI	GCATC	9 cut(s)	532, 705, 785, 1120, 1861, 1974, 2089, 2535, 2667
SfcI	CTRYAG	4 cut(s)	429, 1098, 1209, 2374
SfuI	TTCGAA	1 cut(s)	158
SinI	GGWCC	2 cut(s)	617, 1361
SmiMI	CAYNNNNRTG	5 cut(s)	1452, 1458, 1956, 2163, 2630
SmlI	CTYRAG	2 cut(s)	678, 1714
SmoI	CTYRAG	2 cut(s)	678, 1714
SnaBI	TACGTA	1 cut(s)	2149
SphI	GCATGC	1 cut(s)	1457
SsiI	CCGC	8 cut(s)	78, 100, 195, 545, 1369, 1833, 2748, 2887
SspMI	CTAG	3 cut(s)	623, 1347, 1569
StyD4I	CCNGG	5 cut(s)	583, 591, 1501, 2617, 2778
StyI	CCWWGG	3 cut(s)	283, 622, 1957
TaiI	ACGT	5 cut(s)	112, 128, 807, 1804, 2151
TaqI	TCGA	9 cut(s)	94, 158, 216, 378, 2230, 2312, 2326, 2679, 2769
TatI	WGTACW	2 cut(s)	1409, 1547
TauI	GCSGC	2 cut(s)	80, 1371
TseFI	GTSAC	4 cut(s)	1815, 2345, 2483, 2648
TseI	GCWGC	2 cut(s)	1012, 2024
Tsp45I	GTSAC	4 cut(s)	1815, 2345, 2483, 2648
TspGWI	ACGGA	4 cut(s)	19, 43, 131, 1637
Tth111I	GACNNNGTC	1 cut(s)	111
Van91I	CCANNNNNTGG	1 cut(s)	2388
VneI	GTGCAC	1 cut(s)	627
VpaK11BI	GGWCC	2 cut(s)	617, 1361
VspI	ATTAAT	1 cut(s)	2142
XagI	CCTNNNNNAGG	2 cut(s)	2267, 2409
XapI	RAATTY	4 cut(s)	732, 1379, 1749, 2029
XceI	RCATGY	3 cut(s)	1019, 1457, 1554
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1286
XmaJI	CCTAGG	1 cut(s)	622
XmiI	GTMKAC	2 cut(s)	1212, 1864
XmnI	GAANNNNTTC	1 cut(s)	1482
XspI	CTAG	3 cut(s)	623, 1347, 1569
ZraI	GACGTC	1 cut(s)	110
Zsp2I	ATGCAT	1 cut(s)	2097

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.