Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

53854477 .. 53865422

Gene Structure 10946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ58701

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2958 bp

ATGCCTTTATGTACTTGTTCTCCGCTTCTTCCTCCTCCTCCTCCAACGGTCGACAGTCATTCCCTTCCTCACCGCCGCGCCTATTCTTCATCCTCCATACGCCGTCGTTTCACCTCCCAAGCCAAACCCATTTTCTCCTTCCCGCCAAATTCCGCCAAGTTTTCCACAACACCGCTACTTCAATGCTCGTCCTCATTGCCCATGACTGCCGAACAAGACTCCTCTTCAACCTCACAACCCCAGGTGCAGGGAAGCTTGTTGAAATCAAGGGCATTCTGGGTTAGTGAATCAATCATTGCCTGGAATGTGGATGCTGGAAATGATTCGTGCTACTTATTCGCTAGTGGAACTGCGGCGTTGTCATTAACCGGTGATGGAGTTTTGGGTGAGGATGTAAAAGTTCAGCTTCAAGAGGATAGACATGGGCTACCGGAAAATGTGAAAGCAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAGGGTCCCCCCTGATCTGGATGTCAGACCTCTTCTCAAAGCTCAGTTGGCAGTTGCTACTTTCAGTTCTGATGGAAGGTGCAGCGATGCTACTGGTCTGCAGTTACCGGGTGTCCTAGATGAATTGTTCTCGTATGATGGTTCCCTTGGTGCAGTGTATTCAAAAGAAGCTGTGTCCCTTTACCTTTGGGCACCTACTTCTCAGGCAGTATCTGTATTGATCTATAAGGAGCCATCGGGTGGAAATCCCTTAGAAGTTGTGCAGCTTGAGGAAGTTAATGGCATTTGGAGTACTAGAGGACCAAAAACTTGGGAAGGATGCTTCTATGTGTATGAAGTGTCCGTATACCACCCTAGCACCTTACAAATTGAAAAGTGCTATGCAAATGATCCATATGCTAGAGGGCTCTCAGCAGATGGTAGGCGGACATTGTTCATCAATCTTGATTCTGATCATATAAAACCAGAAGGATGGGATAAATTGGTAGATGAGAAACCTGATATACATTCTTCTTCGGACATAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGCGGTGGATATCTGGCATTCACTTCGCAGGACTCGGCTGGCATATCTCATTTGAAGAAATTATCAAATGCTGGTATCACTCATGTCCATCTACTGCCAGCCTACCAATTTGCAGGTGTTGATGATGAGAAGGGGAACTGGAAGAGTGTAGATTCCAAGATTCTTGAAAAGTTTCCTCCAGATTCATCCGAGCAACAAGCTCTAATTACAGCAATCCAAAATGATGATGGGTATAACTGGGGGTACAACCCTGTCCTTTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTTCATACCGGGCAATTGAGTTTAGGAAGATGGTTCAGGCACTTAATCGTTTTGGCCTTCGTGTTGTCTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGAAAATTCAGTTCTTGATAAGATTGTTCCAGGTTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAATAGCACATGTGTAAACAACACTGCTAGTGAGCATTTTATGGTAGAGCGTTTAATTCTTGATGATCTTTTGCACTGGGCAGTTGATTTTAAGGTTGATGGATTCCGGTTTGATCTTATGGGTCATATAATGAAAAGAACAATGGTGAAAGCAAAAGATGCGCTTTACAGCCTAACAAAGGATAGGGATGGAGTTGATGGCTCAAGTATCTATATATATGGTGAAGGATGGGATTTTGGTGAAGTGGCCAACAATGGGCGTGGAGTAAATGCATCGCAGTTCAATATTTGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTGGGTGGTTCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCCGAAGTTGTTGCAGAACGTATGCTTGCTGAATCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTGAGGGACTTTGTGCTAACCAATTATGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGTGGGACAAGTGTAGCATATGCTTTGGACCCTACTGAAACTGTTAATTATGCTTCAGCTCATGACAACGAAACTCTTTTTGACATCGTGAGTCTGAAGACTCCATTTGAAATTTCTGTAGAAGAGAGATGCAGGATAAATCTCTTGGCGTCAAGCATAATAGCACTATCACAGGGAATTCCATTTTTCCACTCCGGTGATGAGATGCTCCGGTCCAAATCACTTGATCGCGACTCATACAATTCTGGTGATTGGTTCAACAGGATTGACTTCACTTATAATTCCAACAATTGGGGTGTTGGCATTCCTCCAAAGGAGAAAAATGAAAAGAGCTGGCCACTAATGAAACCAAGATTAGCAGAGCCATCCTTTAAGCCTCAGAAGAGTCACATCCATGCTGCTGTGGAGAATTTTTCAGATTTGTTACGTATTAGGTACTCTTCACCACTTTTCCGTCTGAGGACAGCAAATGCTATTCAGGAACGCGTACGTTTCCACAATACTGGGCCTGCATCCGTCCCTGGCATCATAGTAATGAGCATTGAAGATGGTCATGAAGGACTACCAGGGTTATCTCAAATGGATCCAATCTACTCCTACATTGTGGTTATCGTCAATGCACGCCCAACTGAGGTGTCATTTACAAGTTCTTCTATTCGTGCTAGGACCCTTCAATTACATCCTGTACAGGTTAAGTCCAATGATGAAATAGTGAAGAGATCTACTCATGACATAGGCTCGGGATGCTTTACTGTGCCCGCAAGGACGACATCTGTGTTTGTCGAGCCTCGGGGGTATGAGTTGCTCTCTGCTGCATGGTTCACTAAGAATGTTATAAATGCGACAATTGACCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

985

Amino Acids

109.06

Weight (kDa)

5.69

Isoelectric Point (pI)

39.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	88 - 201	2e-33	Pullulanase N2 domain
CBM_48	PF02922	209 - 293	8.6e-07	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	793 - 962	1.1e-64	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2379, 2936
AarI	CACCTGC	1 cut(s)	1169
Acc36I	ACCTGC	1 cut(s)	1169
AccB1I	GGYRCC	1 cut(s)	670
AccB7I	CCANNNNNTGG	2 cut(s)	719, 2391
AccI	GTMKAC	4 cut(s)	51, 825, 1443, 1933
AccII	CGCG	4 cut(s)	78, 1068, 2331, 2586
AclWI	GGATC	5 cut(s)	863, 2013, 2089, 2678, 2691
AcoI	YGGCCR	3 cut(s)	1791, 1900, 2435
AcsI	RAATTY	6 cut(s)	148, 1061, 1480, 2211, 2277, 2509
AcuI	CTGAAG	3 cut(s)	1895, 2139, 2216
AcyI	GRCGYC	1 cut(s)	2249
AfaI	GTAC	7 cut(s)	13, 772, 1310, 1840, 2537, 2589, 2787
AfiI	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2731
AflIII	ACRYGT	2 cut(s)	1553, 2584
AgeI	ACCGGT	1 cut(s)	368
AjnI	CCWGG	5 cut(s)	240, 299, 1504, 2620, 2665
AjuI	GAANNNNNNNTTGG	2 cut(s)	116, 148
AleI	CACNNNNGTG	1 cut(s)	2295
AluBI	AGCT	9 cut(s)	255, 406, 521, 650, 745, 1265, 1344, 2159, 2433
AluI	AGCT	9 cut(s)	255, 406, 521, 650, 745, 1265, 1344, 2159, 2433
AlwI	GGATC	5 cut(s)	863, 2013, 2089, 2678, 2691
AlwNI	CAGNNNCTG	1 cut(s)	692
Ama87I	CYCGRG	2 cut(s)	2839, 2889
AoxI	GGCC	7 cut(s)	1417, 1466, 1791, 1900, 1963, 2435, 2606
ApeKI	GCWGC	5 cut(s)	561, 742, 2027, 2498, 2912
ApoI	RAATTY	6 cut(s)	148, 1061, 1480, 2211, 2277, 2509
AsiGI	ACCGGT	1 cut(s)	368
Asp700I	GAANNNNTTC	2 cut(s)	322, 1236
AspLEI	GCGC	2 cut(s)	80, 1708
AspS9I	GGNCC	9 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313, 2606, 2766
AsuC2I	CCSGG	2 cut(s)	588, 1373
AvaI	CYCGRG	2 cut(s)	2839, 2889
AvaII	GGWCC	6 cut(s)	484, 779, 1330, 2128, 2313, 2766
BaeGI	GKGCMC	2 cut(s)	673, 2859
BalI	TGGCCA	3 cut(s)	1793, 1902, 2437
BamHI	GGATCC	1 cut(s)	2683
BanI	GGYRCC	1 cut(s)	670
BanII	GRGCYC	1 cut(s)	888
BbsI	GAAGAC	1 cut(s)	2204
BbvI	GCAGC	5 cut(s)	573, 754, 2014, 2485, 2899
BceAI	ACGGC	1 cut(s)	87
BcgI	CGANNNNNNTGC	4 cut(s)	1071, 1105, 1958, 1992
BciT130I	CCWGG	5 cut(s)	242, 301, 1506, 2622, 2667
BclI	TGATCA	1 cut(s)	931
BcnI	CCSGG	2 cut(s)	588, 1373
BfaI	CTAG	7 cut(s)	342, 596, 774, 834, 879, 1572, 2763
BfmI	CTRYAG	3 cut(s)	469, 578, 2217
BfuAI	ACCTGC	1 cut(s)	1169
BglII	AGATCT	1 cut(s)	2819
BisI	GCNGC	7 cut(s)	76, 354, 562, 743, 2028, 2499, 2913
BlsI	GCNGC	7 cut(s)	77, 355, 563, 744, 2029, 2500, 2914
BmcAI	AGTACT	1 cut(s)	772
Bme1390I	CCNGG	7 cut(s)	242, 301, 588, 1373, 1506, 2622, 2667
Bme18I	GGWCC	6 cut(s)	484, 779, 1330, 2128, 2313, 2766
BmeT110I	CYCGRG	2 cut(s)	2839, 2889
BmgT120I	GGNCC	9 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313, 2606, 2766
BmrFI	CCNGG	7 cut(s)	242, 301, 588, 1373, 1506, 2622, 2667
BmrI	ACTGGG	4 cut(s)	1312, 1630, 1892, 2613
BmuI	ACTGGG	4 cut(s)	1312, 1630, 1892, 2613
BpiI	GAAGAC	1 cut(s)	2204
BplI	GAGNNNNNCTC	4 cut(s)	2809, 2841, 2891, 2923
BpmI	CTGGAG	1 cut(s)	1227
BpuEI	CTTGAG	4 cut(s)	767, 1537, 1732, 2056
BpuMI	CCSGG	2 cut(s)	588, 1373
BsaAI	YACGTR	1 cut(s)	2528
BsaBI	GATNNNNATC	1 cut(s)	930
BsaHI	GRCGYC	1 cut(s)	2249
BsaJI	CCNNGG	5 cut(s)	240, 625, 2620, 2666, 2888
BsaWI	WCCGGW	5 cut(s)	368, 430, 1650, 2294, 2310
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2731
Bse118I	RCCGGY	1 cut(s)	368
Bse1I	ACTGG	9 cut(s)	577, 1208, 1307, 1625, 1846, 1887, 1933, 2608, 2953
Bse3DI	GCAATG	2 cut(s)	194, 294
Bse8I	GATNNNNATC	1 cut(s)	930
BseBI	CCWGG	5 cut(s)	242, 301, 1506, 2622, 2667
BseDI	CCNNGG	5 cut(s)	240, 625, 2620, 2666, 2888
BseJI	GATNNNNATC	1 cut(s)	930
BseLI	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2731
BseMI	GCAATG	2 cut(s)	194, 294
BseMII	CTCAG	6 cut(s)	536, 695, 903, 2492, 2549, 2721
BseNI	ACTGG	9 cut(s)	577, 1208, 1307, 1625, 1846, 1887, 1933, 2608, 2953
BseRI	GAGGAG	4 cut(s)	24, 27, 30, 211
BseSI	GKGCMC	2 cut(s)	673, 2859
BseXI	GCAGC	5 cut(s)	573, 754, 2014, 2485, 2899
BsgI	GTGCAG	4 cut(s)	266, 580, 651, 761
Bsh1236I	CGCG	4 cut(s)	78, 1068, 2331, 2586
Bsh1285I	CGRYCG	1 cut(s)	51
BshFI	GGCC	7 cut(s)	1419, 1468, 1793, 1902, 1965, 2437, 2608
BshNI	GGYRCC	1 cut(s)	670
BshTI	ACCGGT	1 cut(s)	368
BsiEI	CGRYCG	1 cut(s)	51
BsiHKCI	CYCGRG	2 cut(s)	2839, 2889
BsiSI	CCGG	7 cut(s)	369, 431, 587, 1372, 1651, 2295, 2311
BsiWI	CGTACG	1 cut(s)	2587
BslFI	GGGAC	6 cut(s)	470, 640, 1316, 2054, 2119, 2603
BslI	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2731
BsmFI	GGGAC	6 cut(s)	470, 640, 1316, 2054, 2119, 2603
BsmI	GAATGC	3 cut(s)	272, 1082, 2403
BsnI	GGCC	7 cut(s)	1419, 1468, 1793, 1902, 1965, 2437, 2608
BsoBI	CYCGRG	2 cut(s)	2839, 2889
Bsp1286I	GDGCHC	3 cut(s)	673, 888, 2859
Bsp1407I	TGTACA	1 cut(s)	2785
Bsp68I	TCGCGA	1 cut(s)	2331
BspANI	GGCC	7 cut(s)	1419, 1468, 1793, 1902, 1965, 2437, 2608
BspCNI	CTCAG	6 cut(s)	535, 694, 902, 2491, 2550, 2722
BspFNI	CGCG	4 cut(s)	78, 1068, 2331, 2586
BspHI	TCATGA	3 cut(s)	2161, 2653, 2827
BspMAI	CTGCAG	1 cut(s)	582
BspMI	ACCTGC	1 cut(s)	1169
BspPI	GGATC	5 cut(s)	863, 2013, 2089, 2678, 2691
BspT107I	GGYRCC	1 cut(s)	670
BsrDI	GCAATG	2 cut(s)	194, 294
BsrFI	RCCGGY	1 cut(s)	368
BsrGI	TGTACA	1 cut(s)	2785
BsrI	ACTGG	9 cut(s)	577, 1208, 1307, 1625, 1846, 1887, 1933, 2608, 2953
BssAI	RCCGGY	1 cut(s)	368
BssECI	CCNNGG	5 cut(s)	240, 625, 2620, 2666, 2888
BssNAI	GTATAC	1 cut(s)	826
BssNI	GRCGYC	1 cut(s)	2249
BssT1I	CCWWGG	1 cut(s)	625
Bst1107I	GTATAC	1 cut(s)	826
Bst2UI	CCWGG	5 cut(s)	242, 301, 1506, 2622, 2667
Bst4CI	ACNGT	5 cut(s)	49, 56, 1051, 2143, 2854
Bst6I	CTCTTC	8 cut(s)	229, 516, 1202, 1914, 2217, 2477, 2545, 2810
BstACI	GRCGYC	1 cut(s)	2249
BstAPI	GCANNNNNTGC	2 cut(s)	1355, 1703
BstAUI	TGTACA	1 cut(s)	2785
BstBAI	YACGTR	1 cut(s)	2528
BstC8I	GCNNGC	7 cut(s)	1105, 1164, 1992, 2435, 2610, 2722, 2859
BstDEI	CTNAG	8 cut(s)	522, 681, 730, 889, 2478, 2558, 2730, 2925
BstENI	CCTNNNNNAGG	1 cut(s)	1721
BstFNI	CGCG	4 cut(s)	78, 1068, 2331, 2586
BstHHI	GCGC	2 cut(s)	80, 1708
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	4 cut(s)	527, 1355, 1703, 2844
BstNI	CCWGG	5 cut(s)	242, 301, 1506, 2622, 2667
BstNSI	RCATGY	1 cut(s)	1557
BstSCI	CCNGG	7 cut(s)	240, 299, 586, 1371, 1504, 2620, 2665
BstSFI	CTRYAG	3 cut(s)	469, 578, 2217
BstSLI	GKGCMC	2 cut(s)	673, 2859
BstSNI	TACGTA	1 cut(s)	2528
BstUI	CGCG	4 cut(s)	78, 1068, 2331, 2586
BstV1I	GCAGC	5 cut(s)	573, 754, 2014, 2485, 2899
BstV2I	GAAGAC	1 cut(s)	2204
BstX2I	RGATCY	2 cut(s)	2683, 2819
BstXI	CCANNNNNNTGG	3 cut(s)	789, 951, 2603
BstYI	RGATCY	2 cut(s)	2683, 2819
BstZ17I	GTATAC	1 cut(s)	826
BsuRI	GGCC	7 cut(s)	1419, 1468, 1793, 1902, 1965, 2437, 2608
BtgZI	GCGATG	2 cut(s)	579, 1803
BtsI	GCAGTG	2 cut(s)	639, 1566
BtsIMutI	CAGTG	6 cut(s)	639, 1039, 1056, 1527, 1566, 1618
BtuMI	TCGCGA	1 cut(s)	2331
BveI	ACCTGC	1 cut(s)	1169
Cac8I	GCNNGC	7 cut(s)	1105, 1164, 1992, 2435, 2610, 2722, 2859
CaiI	CAGNNNCTG	1 cut(s)	692
CciI	TCATGA	3 cut(s)	2161, 2653, 2827
CfoI	GCGC	2 cut(s)	80, 1708
Cfr10I	RCCGGY	1 cut(s)	368
Cfr13I	GGNCC	9 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313, 2606, 2766
CseI	GACGC	1 cut(s)	2238
Csp6I	GTAC	7 cut(s)	12, 771, 1309, 1839, 2536, 2588, 2786
CspAI	ACCGGT	1 cut(s)	368
CspCI	CAANNNNNGTGG	4 cut(s)	1786, 1816, 1821, 1851
CviQI	GTAC	7 cut(s)	12, 771, 1309, 1839, 2536, 2588, 2786
DdeI	CTNAG	8 cut(s)	522, 681, 730, 889, 2478, 2558, 2730, 2925
EaeI	YGGCCR	3 cut(s)	1791, 1900, 2435
Eam1104I	CTCTTC	8 cut(s)	229, 516, 1202, 1914, 2217, 2477, 2545, 2810
EarI	CTCTTC	8 cut(s)	229, 516, 1202, 1914, 2217, 2477, 2545, 2810
EciI	GGCGGA	2 cut(s)	142, 919
Eco105I	TACGTA	1 cut(s)	2528
Eco130I	CCWWGG	1 cut(s)	625
Eco24I	GRGCYC	1 cut(s)	888
Eco32I	GATATC	1 cut(s)	1076
Eco47I	GGWCC	6 cut(s)	484, 779, 1330, 2128, 2313, 2766
Eco57I	CTGAAG	3 cut(s)	1895, 2139, 2216
Eco88I	CYCGRG	2 cut(s)	2839, 2889
EcoNI	CCTNNNNNAGG	1 cut(s)	1721
EcoO109I	RGGNCCY	3 cut(s)	484, 1330, 2766
EcoRI	GAATTC	1 cut(s)	2277
EcoRII	CCWGG	5 cut(s)	240, 299, 1504, 2620, 2665
EcoRV	GATATC	1 cut(s)	1076
EcoT14I	CCWWGG	1 cut(s)	625
EcoT22I	ATGCAT	1 cut(s)	1819
EcoT38I	GRGCYC	1 cut(s)	888
ErhI	CCWWGG	1 cut(s)	625
FalI	AAGNNNNNCTT	4 cut(s)	390, 422, 1692, 1724
FaqI	GGGAC	6 cut(s)	470, 640, 1316, 2054, 2119, 2603
FauI	CCCGC	2 cut(s)	150, 2866
FauNDI	CATATG	3 cut(s)	874, 2098, 2119
FbaI	TGATCA	1 cut(s)	931
FblI	GTMKAC	4 cut(s)	51, 825, 1443, 1933
Fnu4HI	GCNGC	7 cut(s)	76, 354, 562, 743, 2028, 2499, 2913
FriOI	GRGCYC	1 cut(s)	888
Fsp4HI	GCNGC	7 cut(s)	76, 354, 562, 743, 2028, 2499, 2913
FspBI	CTAG	7 cut(s)	342, 596, 774, 834, 879, 1572, 2763
GlaI	GCGC	2 cut(s)	79, 1707
GluI	GCNGC	7 cut(s)	76, 354, 562, 743, 2028, 2499, 2913
GsuI	CTGGAG	1 cut(s)	1227
HaeIII	GGCC	7 cut(s)	1419, 1468, 1793, 1902, 1965, 2437, 2608
HapII	CCGG	7 cut(s)	369, 431, 587, 1372, 1651, 2295, 2311
HgaI	GACGC	1 cut(s)	2238
HhaI	GCGC	2 cut(s)	80, 1708
Hin1I	GRCGYC	1 cut(s)	2249
Hin6I	GCGC	2 cut(s)	78, 1706
HinP1I	GCGC	2 cut(s)	78, 1706
HincII	GTYRAC	1 cut(s)	52
HindII	GTYRAC	1 cut(s)	52
HindIII	AAGCTT	1 cut(s)	253
HpaII	CCGG	7 cut(s)	369, 431, 587, 1372, 1651, 2295, 2311
Hpy166II	GTNNAC	6 cut(s)	52, 826, 1444, 1561, 1934, 2922
Hpy8I	GTNNAC	6 cut(s)	52, 826, 1444, 1561, 1934, 2922
Hpy99I	CGWCG	1 cut(s)	108
HpyCH4III	ACNGT	5 cut(s)	49, 56, 1051, 2143, 2854
HpyCH4IV	ACGT	3 cut(s)	1984, 2527, 2590
HpyF10VI	GCNNNNNNNGC	4 cut(s)	527, 1355, 1703, 2844
HpyF3I	CTNAG	8 cut(s)	522, 681, 730, 889, 2478, 2558, 2730, 2925
HpySE526I	ACGT	3 cut(s)	1984, 2527, 2590
Hsp92I	GRCGYC	1 cut(s)	2249
HspAI	GCGC	2 cut(s)	78, 1706
KflI	GGGWCCC	2 cut(s)	484, 1330
Ksp22I	TGATCA	1 cut(s)	931
LmnI	GCTCC	2 cut(s)	709, 2313
Lsp1109I	GCAGC	5 cut(s)	573, 754, 2014, 2485, 2899
MaeI	CTAG	7 cut(s)	342, 596, 774, 834, 879, 1572, 2763
MaeII	ACGT	3 cut(s)	1984, 2527, 2590
MaeIII	GTNAC	6 cut(s)	582, 1508, 1924, 1952, 2486, 2523
MfeI	CAATTG	3 cut(s)	1377, 2389, 2946
MflI	RGATCY	2 cut(s)	2683, 2819
MhlI	GDGCHC	3 cut(s)	673, 888, 2859
MlsI	TGGCCA	3 cut(s)	1793, 1902, 2437
MluI	ACGCGT	1 cut(s)	2584
MluNI	TGGCCA	3 cut(s)	1793, 1902, 2437
MlyI	GAGTC	6 cut(s)	212, 1091, 2194, 2200, 2327, 2494
MmeI	TCCRAC	2 cut(s)	68, 2409
Mox20I	TGGCCA	3 cut(s)	1793, 1902, 2437
Mph1103I	ATGCAT	1 cut(s)	1819
MroXI	GAANNNNTTC	2 cut(s)	322, 1236
MscI	TGGCCA	3 cut(s)	1793, 1902, 2437
MseI	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2793
MslI	CAYNNNNRTG	5 cut(s)	1455, 1461, 2295, 2493, 2633
Msp20I	TGGCCA	3 cut(s)	1793, 1902, 2437
MspI	CCGG	7 cut(s)	369, 431, 587, 1372, 1651, 2295, 2311
MspR9I	CCNGG	7 cut(s)	242, 301, 588, 1373, 1506, 2622, 2667
MunI	CAATTG	3 cut(s)	1377, 2389, 2946
Mva1269I	GAATGC	3 cut(s)	272, 1082, 2403
MvaI	CCWGG	5 cut(s)	242, 301, 1506, 2622, 2667
MvnI	CGCG	4 cut(s)	78, 1068, 2331, 2586
MwoI	GCNNNNNNNGC	4 cut(s)	527, 1355, 1703, 2844
NciI	CCSGG	2 cut(s)	588, 1373
NdeI	CATATG	3 cut(s)	874, 2098, 2119
NmeAIII	GCCGAG	1 cut(s)	1079
NmuCI	GTSAC	2 cut(s)	1924, 2486
NruI	TCGCGA	1 cut(s)	2331
NsiI	ATGCAT	1 cut(s)	1819
NspI	RCATGY	1 cut(s)	1557
OliI	CACNNNNGTG	1 cut(s)	2295
PagI	TCATGA	3 cut(s)	2161, 2653, 2827
PaqCI	CACCTGC	1 cut(s)	1169
PciI	ACATGT	1 cut(s)	1553
PctI	GAATGC	3 cut(s)	272, 1082, 2403
PdmI	GAANNNNTTC	2 cut(s)	322, 1236
PfeI	GAWTC	8 cut(s)	287, 323, 926, 1217, 1225, 1247, 1647, 1997
Pfl23II	CGTACG	1 cut(s)	2587
PflMI	CCANNNNNTGG	2 cut(s)	719, 2391
PinAI	ACCGGT	1 cut(s)	368
PkrI	GCNGC	7 cut(s)	77, 355, 563, 744, 2029, 2500, 2914
PleI	GAGTC	6 cut(s)	212, 1091, 2194, 2199, 2327, 2493
PpsI	GAGTC	6 cut(s)	212, 1091, 2194, 2199, 2327, 2493
Ppu21I	YACGTR	1 cut(s)	2528
PpuMI	RGGWCCY	3 cut(s)	484, 1330, 2766
PscI	ACATGT	1 cut(s)	1553
PsiI	TTATAA	2 cut(s)	2379, 2936
Psp5II	RGGWCCY	3 cut(s)	484, 1330, 2766
Psp6I	CCWGG	5 cut(s)	240, 299, 1504, 2620, 2665
PspGI	CCWGG	5 cut(s)	240, 299, 1504, 2620, 2665
PspLI	CGTACG	1 cut(s)	2587
PspPI	GGNCC	9 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313, 2606, 2766
PspPPI	RGGWCCY	3 cut(s)	484, 1330, 2766
PstI	CTGCAG	1 cut(s)	582
PstNI	CAGNNNCTG	1 cut(s)	692
PsuI	RGATCY	2 cut(s)	2683, 2819
RruI	TCGCGA	1 cut(s)	2331
RsaI	GTAC	7 cut(s)	13, 772, 1310, 1840, 2537, 2589, 2787
RsaNI	GTAC	7 cut(s)	12, 771, 1309, 1839, 2536, 2588, 2786
RseI	CAYNNNNRTG	5 cut(s)	1455, 1461, 2295, 2493, 2633
SalI	GTCGAC	1 cut(s)	50
SaqAI	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2793
SatI	GCNGC	7 cut(s)	76, 354, 562, 743, 2028, 2499, 2913
Sau96I	GGNCC	9 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313, 2606, 2766
ScaI	AGTACT	1 cut(s)	772
SchI	GAGTC	6 cut(s)	212, 1091, 2194, 2200, 2327, 2494
ScrFI	CCNGG	7 cut(s)	242, 301, 588, 1373, 1506, 2622, 2667
SduI	GDGCHC	3 cut(s)	673, 888, 2859
SfcI	CTRYAG	3 cut(s)	469, 578, 2217
SinI	GGWCC	6 cut(s)	484, 779, 1330, 2128, 2313, 2766
SmiMI	CAYNNNNRTG	5 cut(s)	1455, 1461, 2295, 2493, 2633
SmlI	CTYRAG	4 cut(s)	746, 1516, 1747, 2035
SmoI	CTYRAG	4 cut(s)	746, 1516, 1747, 2035
SnaBI	TACGTA	1 cut(s)	2528
SspI	AATATT	1 cut(s)	1831
SspMI	CTAG	7 cut(s)	342, 596, 774, 834, 879, 1572, 2763
StyD4I	CCNGG	7 cut(s)	240, 299, 586, 1371, 1504, 2620, 2665
StyI	CCWWGG	1 cut(s)	625
TaaI	ACNGT	5 cut(s)	49, 56, 1051, 2143, 2854
TaiI	ACGT	3 cut(s)	1987, 2530, 2593
TaqI	TCGA	3 cut(s)	51, 1864, 2883
TatI	WGTACW	3 cut(s)	11, 770, 2785
TauI	GCSGC	2 cut(s)	78, 356
TfiI	GAWTC	8 cut(s)	287, 323, 926, 1217, 1225, 1247, 1647, 1997
Tru1I	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2793
Tru9I	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2793
TscAI	CASTG	6 cut(s)	639, 1039, 1056, 1534, 1573, 1625
TseFI	GTSAC	2 cut(s)	1924, 2486
TseI	GCWGC	5 cut(s)	561, 742, 2027, 2498, 2912
Tsp45I	GTSAC	2 cut(s)	1924, 2486
TspGWI	ACGGA	3 cut(s)	811, 2543, 2605
TspRI	CASTG	6 cut(s)	639, 1039, 1056, 1534, 1573, 1625
Van91I	CCANNNNNTGG	2 cut(s)	719, 2391
VpaK11BI	GGWCC	6 cut(s)	484, 779, 1330, 2128, 2313, 2766
XagI	CCTNNNNNAGG	1 cut(s)	1721
XapI	RAATTY	6 cut(s)	148, 1061, 1480, 2211, 2277, 2509
XceI	RCATGY	1 cut(s)	1557
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1289
XmiI	GTMKAC	4 cut(s)	51, 825, 1443, 1933
XmnI	GAANNNNTTC	2 cut(s)	322, 1236
XspI	CTAG	7 cut(s)	342, 596, 774, 834, 879, 1572, 2763
ZrmI	AGTACT	1 cut(s)	772
Zsp2I	ATGCAT	1 cut(s)	1819

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.