Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

15361873 .. 15372412

Gene Structure 10540 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g18370.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2883 bp

ATGGGTATATATACTTGTTCACTTCTTCTTCCTCCTCCAACAGTCAACTCCCATTTCCCTAATCACATCCGCCACGCCTTTTCTTCATCCTCCATACGCTGTCGTTTCACCTCCCACCCCAAACCCAAACCCATCTTCTCCTTCCCGCCAACTTCCGCCAACTTTTCGAGACTTCAATGCTCGGCCTCATTGCCCATGTCTGCCCAGCAAGACTCTGCTTCAACCTCACCATCCCAGGTGGAAGGTAGCTTGTTGAAATCAAGGGCATTCTGGGTCAGTGAATCAATCATTGCCTGGAATGTGGATACTGGAGATGATTCGTGCTACTTGTTCGCTAGTAAAACTGCGGCGTTGTCATTGACTGGTGATGGAGTTTCGGGTGAGGATGTAAAAGTTGAGCTGAGAGAAGATAGACATGGGCTACCGCAAAATGTGAAAGAAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAATGTTCCCTCTGATCTGGATGTCAAATCTCTTCTCAAAGCCCAGTTGGCAGTTGCTACTTTCAGTTGTGATGGAAGGTGCAGCGATGCTACTGGTCTGCAGTTACCGGGCATCCTAGACGAATTATTCTCATATGAAGGTCCCCTTGGTGCAGTGTTTTCAAAAGAAGCTGTGTCACTTTACCTTTGGGCTCCTACTTCTCAGGCAGTATCTGTTTTGATCTATAAGGAGCCACTGGGTGGAAATCCCTTAGAAATTGTGCAGCTTGAGGAAGTTAATGGCGTTTGGAGTACTAGAGGACCAAAAAGTTGGGAAGGATGCTACTATGTGTATGAAGTGTCTGTATACCACCCTAGCACCTTACAAATTGAAAAATGCTATGCAAATGATCCATATGCTAGAGGGCTCTCATCAGATGGCAGGCGGACATTGTTCATCAACCTTGATTCTGACTCTATAAAACCAGATGGATGGGATAAATTGGTAGATGAGAAACCTGATATACTTTCTTCTTCAGACATAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGCGGTGGATATCTGGCTTTCACTTCACAGGACTCCGCTGGTGTATCTCATTTGAAGAAATTATCAAAGGCTGGTATGACTCATGTCCATCTACTTCCAGCCTTCCAGTTTTCAGGTGTTGATGATGAAAAGGAGAACTGGAAAAGCATAGATTCCAAGATTCTTGAAAAATTTCCTTCAGATTCATCAGAGCAACAAGCTCTAATTACAGCTATCCAAAATGATGATGGTTATAACTGGGGGTACAACCCTGTTCTCTGGGGGGTCCCTAAGGGAAGCTATGCAAGTAATGCAAATGGTTCATACCGTGCACGTGAGTTTAGAAAGATGGTTCAGGCACTTAATCGTCTTGGCCTTCGTGTTGTTTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGCAAATTCAGTTCTCGATAAGATTGTTCCAGGGTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAACAGTACATGTGTAAACAACACTGCTAGCGAGCATTTTATGGTTGAACGTTTAATTCTTGATGATCTTTTACACTGGGCGGTTGATTATAAGGTTGATGGATTCCGGTTTGACCTTATGGGTCATATGATGAAAAGAACAATGGTGAAAGCAAAAGATGCACTCTGCAGCCTAACAAAGGATAGGGATGGAGTTGATGGCTCAAGTATCTACATATATGGTGAAGGATGGGATTTTGGTGAAGTTGCCAACAATGGGCGTGGAGTAAATGCATCGCAGTTCAATATTTGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATTCTGGGTGGTTCTCCTTTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCAGAAGCTGTTGCAGAACGCATGCTTGCTGAAGCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTAAGGGACTTTGTGCTAACCAATTGTGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGCGGGACAAGTGTAGCATATGCTTTAGACCCTACTGAAACGGTTAATTATGCTTCGGCTCATGACAATGAAACTCTGTTTGACATCACTCCATTTGAAATTTCCGTAGAAGAGAGATGCAGGATAAATTTCTTGGCGACAAGCATAATAGCTCTATCACAGGGTATACCATTTTTCCACTGCGGTGATGAGATTCTCCGGTCAAAATCACTTGATCGCGACTCATACAATTCTGGTGATTGGTTCAACAGGATCGACTTCACCTATAATTCCAACAATTGGGGTGTTGGCCTTCCTCCAAAAGAGAAAAATGAAAAGAGCTGGCCACTAATGAAACCAAGATTAGCAGAGCCATCCTTCAAGCCTCAGAAGAGTCACATCCATGCTGCTGTAGAAAATTTTTCAGATTTGTTACATATTAGGTACTCTTCACCACTTTTCCGTCTGAGGACAGCAAATGCTATTCAGGAACGCTTACGTTTCCACAATACTGGGCCTTCATCAGTCCCTGGTCTCATACTCATGAGCATTGAAGATGGTCATGAAGGACTACCAGGGTTATCTCAACTGGATCCAATCTACTCATACATTGTGGTTATCGTCAATGCTCGCCCAACTGAGGTGTCATTTACAAGTTCTTCCATTCGGGGTAGGGCCCTTCAATTGCATCCTGTACAGGTCAAGTCAAATGATGAAATAGTGAAGAAATCTACTTATGACACAGGCTCCGCATGCTTTACTGTGCCCGCAAGGACGACATCTGTGTTTGTCGAGCCTCGGGGGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

961

Amino Acids

106.26

Weight (kDa)

5.69

Isoelectric Point (pI)

36.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullul_strch_C	PF11852	570 - 739	4.2e-65	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1293, 1629
AccB7I	CCANNNNNTGG	2 cut(s)	713, 2373
AccI	GTMKAC	4 cut(s)	819, 1437, 1927, 2260
AccII	CGCG	2 cut(s)	1062, 2313
AclI	AACGTT	1 cut(s)	1588
AclWI	GGATC	6 cut(s)	857, 2007, 2083, 2354, 2660, 2673
AcoI	YGGCCR	2 cut(s)	1894, 2417
AcsI	RAATTY	6 cut(s)	1055, 1229, 1474, 2193, 2221, 2491
AcuI	CTGAAG	4 cut(s)	972, 1221, 1889, 2010
AcvI	CACGTG	1 cut(s)	1373
AdeI	CACNNNGTG	1 cut(s)	713
AfaI	GTAC	7 cut(s)	766, 1304, 1505, 1546, 1834, 2519, 2769
AfiI	CCNNNNNNNGG	5 cut(s)	713, 1717, 1794, 2373, 2713
AflII	CTTAAG	1 cut(s)	2029
AflIII	ACRYGT	1 cut(s)	1547
AjnI	CCWGG	5 cut(s)	234, 293, 1498, 2602, 2647
AjuI	GAANNNNNNNTTGG	4 cut(s)	119, 151, 603, 635
AleI	CACNNNNGTG	1 cut(s)	2277
Alw21I	GWGCWC	1 cut(s)	1372
Alw26I	GTCTC	2 cut(s)	163, 2612
Alw44I	GTGCAC	1 cut(s)	1368
AlwI	GGATC	6 cut(s)	857, 2007, 2083, 2354, 2660, 2673
AlwNI	CAGNNNCTG	3 cut(s)	686, 1967, 2603
Ama87I	CYCGRG	1 cut(s)	2871
AoxI	GGCC	9 cut(s)	183, 1411, 1460, 1894, 1957, 2383, 2417, 2588, 2748
ApaI	GGGCCC	1 cut(s)	2752
ApaLI	GTGCAC	1 cut(s)	1368
ApeKI	GCWGC	5 cut(s)	555, 736, 1707, 2021, 2480
ApoI	RAATTY	6 cut(s)	1055, 1229, 1474, 2193, 2221, 2491
Asp700I	GAANNNNTTC	2 cut(s)	444, 1230
AspS9I	GGNCC	8 cut(s)	614, 773, 1324, 1460, 1958, 2588, 2748, 2749
AsuC2I	CCSGG	1 cut(s)	582
AsuNHI	GCTAGC	1 cut(s)	1565
AvaI	CYCGRG	1 cut(s)	2871
AvaII	GGWCC	3 cut(s)	614, 773, 1324
AxyI	CCTNAGG	1 cut(s)	1329
BaeGI	GKGCMC	3 cut(s)	1372, 2752, 2841
BalI	TGGCCA	2 cut(s)	1896, 2419
BamHI	GGATCC	1 cut(s)	2665
BanII	GRGCYC	3 cut(s)	667, 882, 2752
BbrPI	CACGTG	1 cut(s)	1373
Bbv12I	GWGCWC	1 cut(s)	1372
BbvI	GCAGC	5 cut(s)	567, 748, 1719, 2008, 2467
BciT130I	CCWGG	5 cut(s)	236, 295, 1500, 2604, 2649
BciVI	GTATCC	1 cut(s)	298
BcnI	CCSGG	1 cut(s)	582
BcoDI	GTCTC	2 cut(s)	163, 2612
BfaI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
BfmI	CTRYAG	4 cut(s)	463, 572, 1705, 2484
BfrI	CTTAAG	1 cut(s)	2029
BfuI	GTATCC	1 cut(s)	298
BglI	GCCNNNNNGGC	1 cut(s)	521
BisI	GCNGC	6 cut(s)	348, 556, 737, 1708, 2022, 2481
BlsI	GCNGC	6 cut(s)	349, 557, 738, 1709, 2023, 2482
BmcAI	AGTACT	1 cut(s)	766
Bme1390I	CCNGG	6 cut(s)	236, 295, 582, 1500, 2604, 2649
Bme18I	GGWCC	3 cut(s)	614, 773, 1324
BmeT110I	CYCGRG	1 cut(s)	2871
BmgT120I	GGNCC	8 cut(s)	614, 773, 1324, 1460, 1958, 2588, 2748, 2749
BmiI	GGNNCC	8 cut(s)	616, 666, 705, 1325, 1326, 2667, 2750, 2821
BmrFI	CCNGG	6 cut(s)	236, 295, 582, 1500, 2604, 2649
BmrI	ACTGGG	5 cut(s)	511, 719, 1306, 1624, 2595
BmsI	GCATC	9 cut(s)	550, 594, 782, 1459, 1687, 1820, 1858, 2201, 2770
BmtI	GCTAGC	1 cut(s)	1569
BmuI	ACTGGG	5 cut(s)	511, 719, 1306, 1624, 2595
BoxI	GACNNNNGTC	1 cut(s)	1142
BpmI	CTGGAG	1 cut(s)	330
BpuEI	CTTGAG	3 cut(s)	761, 1531, 1726
BpuMI	CCSGG	1 cut(s)	582
BsaAI	YACGTR	1 cut(s)	1373
BsaI	GGTCTC	1 cut(s)	2612
BsaJI	CCNNGG	6 cut(s)	234, 619, 1499, 2602, 2648, 2870
BsaWI	WCCGGW	2 cut(s)	1644, 2292
BsaXI	ACNNNNNCTCC	2 cut(s)	2804, 2834
Bsc4I	CCNNNNNNNGG	5 cut(s)	713, 1717, 1794, 2373, 2713
Bse21I	CCTNAGG	1 cut(s)	1329
Bse3DI	GCAATG	2 cut(s)	188, 288
BseBI	CCWGG	5 cut(s)	236, 295, 1500, 2604, 2649
BseDI	CCNNGG	6 cut(s)	234, 619, 1499, 2602, 2648, 2870
BseLI	CCNNNNNNNGG	5 cut(s)	713, 1717, 1794, 2373, 2713
BseMI	GCAATG	2 cut(s)	188, 288
BseMII	CTCAG	5 cut(s)	392, 689, 2474, 2531, 2703
BseRI	GAGGAG	1 cut(s)	24
BseSI	GKGCMC	3 cut(s)	1372, 2752, 2841
BseXI	GCAGC	5 cut(s)	567, 748, 1719, 2008, 2467
BseYI	CCCAGC	1 cut(s)	204
BsgI	GTGCAG	3 cut(s)	574, 645, 755
Bsh1236I	CGCG	2 cut(s)	1062, 2313
BshFI	GGCC	9 cut(s)	185, 1413, 1462, 1896, 1959, 2385, 2419, 2590, 2750
BsiHKAI	GWGCWC	1 cut(s)	1372
BsiHKCI	CYCGRG	1 cut(s)	2871
BsiSI	CCGG	3 cut(s)	581, 1645, 2293
BslFI	GGGAC	5 cut(s)	600, 1310, 2048, 2113, 2585
BslI	CCNNNNNNNGG	5 cut(s)	713, 1717, 1794, 2373, 2713
BsmAI	GTCTC	2 cut(s)	163, 2612
BsmFI	GGGAC	5 cut(s)	600, 1310, 2048, 2113, 2585
BsmI	GAATGC	1 cut(s)	266
BsnI	GGCC	9 cut(s)	185, 1413, 1462, 1896, 1959, 2385, 2419, 2590, 2750
Bso31I	GGTCTC	1 cut(s)	2612
BsoBI	CYCGRG	1 cut(s)	2871
Bsp120I	GGGCCC	1 cut(s)	2748
Bsp1286I	GDGCHC	5 cut(s)	667, 882, 1372, 2752, 2841
Bsp1407I	TGTACA	1 cut(s)	2767
Bsp68I	TCGCGA	1 cut(s)	2313
BspANI	GGCC	9 cut(s)	185, 1413, 1462, 1896, 1959, 2385, 2419, 2590, 2750
BspCNI	CTCAG	5 cut(s)	393, 688, 2473, 2532, 2704
BspFNI	CGCG	2 cut(s)	1062, 2313
BspHI	TCATGA	3 cut(s)	2155, 2616, 2635
BspLI	GGNNCC	8 cut(s)	616, 666, 705, 1325, 1326, 2667, 2750, 2821
BspMAI	CTGCAG	2 cut(s)	576, 1709
BspOI	GCTAGC	1 cut(s)	1569
BspPI	GGATC	6 cut(s)	857, 2007, 2083, 2354, 2660, 2673
BspTI	CTTAAG	1 cut(s)	2029
BspTNI	GGTCTC	1 cut(s)	2612
BsrDI	GCAATG	2 cut(s)	188, 288
BsrGI	TGTACA	1 cut(s)	2767
BssECI	CCNNGG	6 cut(s)	234, 619, 1499, 2602, 2648, 2870
BssNAI	GTATAC	2 cut(s)	820, 2261
BssT1I	CCWWGG	1 cut(s)	619
Bst1107I	GTATAC	2 cut(s)	820, 2261
Bst2UI	CCWGG	5 cut(s)	236, 295, 1500, 2604, 2649
Bst4CI	ACNGT	6 cut(s)	43, 1045, 1367, 1544, 2137, 2836
Bst6I	CTCTTC	5 cut(s)	510, 1908, 2199, 2459, 2527
BstAFI	CTTAAG	1 cut(s)	2029
BstAPI	GCANNNNNTGC	2 cut(s)	1349, 1697
BstAUI	TGTACA	1 cut(s)	2767
BstBAI	YACGTR	1 cut(s)	1373
BstC8I	GCNNGC	9 cut(s)	896, 1567, 1571, 1982, 1986, 2417, 2704, 2827, 2841
BstDEI	CTNAG	7 cut(s)	401, 675, 724, 1329, 2460, 2540, 2712
BstENI	CCTNNNNNAGG	1 cut(s)	1715
BstFNI	CGCG	2 cut(s)	1062, 2313
BstMAI	GTCTC	2 cut(s)	163, 2612
BstMWI	GCNNNNNNNGC	4 cut(s)	521, 1349, 1697, 2826
BstNI	CCWGG	5 cut(s)	236, 295, 1500, 2604, 2649
BstNSI	RCATGY	3 cut(s)	1551, 1984, 2829
BstPAI	GACNNNNGTC	1 cut(s)	1142
BstSCI	CCNGG	6 cut(s)	234, 293, 580, 1498, 2602, 2647
BstSFI	CTRYAG	4 cut(s)	463, 572, 1705, 2484
BstSLI	GKGCMC	3 cut(s)	1372, 2752, 2841
BstUI	CGCG	2 cut(s)	1062, 2313
BstV1I	GCAGC	5 cut(s)	567, 748, 1719, 2008, 2467
BstX2I	RGATCY	1 cut(s)	2665
BstXI	CCANNNNNNTGG	3 cut(s)	783, 945, 2585
BstYI	RGATCY	1 cut(s)	2665
BstZ17I	GTATAC	2 cut(s)	820, 2261
Bsu36I	CCTNAGG	1 cut(s)	1329
BsuI	GTATCC	1 cut(s)	298
BsuRI	GGCC	9 cut(s)	185, 1413, 1462, 1896, 1959, 2385, 2419, 2590, 2750
BtgZI	GCGATG	2 cut(s)	573, 1797
BtsI	GCAGTG	3 cut(s)	633, 1560, 2272
BtsIMutI	CAGTG	9 cut(s)	283, 633, 707, 1033, 1050, 1521, 1560, 1612, 2272
BtuMI	TCGCGA	1 cut(s)	2313
Cac8I	GCNNGC	9 cut(s)	896, 1567, 1571, 1982, 1986, 2417, 2704, 2827, 2841
CaiI	CAGNNNCTG	3 cut(s)	686, 1967, 2603
CciI	TCATGA	3 cut(s)	2155, 2616, 2635
Cfr13I	GGNCC	8 cut(s)	614, 773, 1324, 1460, 1958, 2588, 2748, 2749
Csp6I	GTAC	7 cut(s)	765, 1303, 1504, 1545, 1833, 2518, 2768
CspCI	CAANNNNNGTGG	4 cut(s)	1780, 1810, 1815, 1845
CviQI	GTAC	7 cut(s)	765, 1303, 1504, 1545, 1833, 2518, 2768
DdeI	CTNAG	7 cut(s)	401, 675, 724, 1329, 2460, 2540, 2712
DraIII	CACNNNGTG	1 cut(s)	713
EaeI	YGGCCR	2 cut(s)	1894, 2417
Eam1104I	CTCTTC	5 cut(s)	510, 1908, 2199, 2459, 2527
EarI	CTCTTC	5 cut(s)	510, 1908, 2199, 2459, 2527
EciI	GGCGGA	3 cut(s)	59, 145, 913
Eco130I	CCWWGG	1 cut(s)	619
Eco24I	GRGCYC	3 cut(s)	667, 882, 2752
Eco31I	GGTCTC	1 cut(s)	2612
Eco32I	GATATC	1 cut(s)	1070
Eco47I	GGWCC	3 cut(s)	614, 773, 1324
Eco57I	CTGAAG	4 cut(s)	972, 1221, 1889, 2010
Eco72I	CACGTG	1 cut(s)	1373
Eco81I	CCTNAGG	1 cut(s)	1329
Eco88I	CYCGRG	1 cut(s)	2871
EcoNI	CCTNNNNNAGG	1 cut(s)	1715
EcoO109I	RGGNCCY	4 cut(s)	614, 1324, 2748, 2749
EcoRII	CCWGG	5 cut(s)	234, 293, 1498, 2602, 2647
EcoRV	GATATC	1 cut(s)	1070
EcoT14I	CCWWGG	1 cut(s)	619
EcoT22I	ATGCAT	1 cut(s)	1813
EcoT38I	GRGCYC	3 cut(s)	667, 882, 2752
ErhI	CCWWGG	1 cut(s)	619
FalI	AAGNNNNNCTT	2 cut(s)	603, 635
FaqI	GGGAC	5 cut(s)	600, 1310, 2048, 2113, 2585
FauI	CCCGC	3 cut(s)	153, 2090, 2848
FauNDI	CATATG	5 cut(s)	607, 868, 1665, 2092, 2113
FblI	GTMKAC	4 cut(s)	819, 1437, 1927, 2260
Fnu4HI	GCNGC	6 cut(s)	348, 556, 737, 1708, 2022, 2481
FriOI	GRGCYC	3 cut(s)	667, 882, 2752
Fsp4HI	GCNGC	6 cut(s)	348, 556, 737, 1708, 2022, 2481
FspBI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
GluI	GCNGC	6 cut(s)	348, 556, 737, 1708, 2022, 2481
GsaI	CCCAGC	1 cut(s)	208
GsuI	CTGGAG	1 cut(s)	330
HaeIII	GGCC	9 cut(s)	185, 1413, 1462, 1896, 1959, 2385, 2419, 2590, 2750
HapII	CCGG	3 cut(s)	581, 1645, 2293
HincII	GTYRAC	1 cut(s)	46
HindII	GTYRAC	1 cut(s)	46
HpaII	CCGG	3 cut(s)	581, 1645, 2293
Hpy166II	GTNNAC	8 cut(s)	20, 46, 820, 1370, 1438, 1555, 1928, 2261
Hpy8I	GTNNAC	8 cut(s)	20, 46, 820, 1370, 1438, 1555, 1928, 2261
HpyCH4III	ACNGT	6 cut(s)	43, 1045, 1367, 1544, 2137, 2836
HpyCH4IV	ACGT	3 cut(s)	1372, 1588, 2572
HpyF10VI	GCNNNNNNNGC	4 cut(s)	521, 1349, 1697, 2826
HpyF3I	CTNAG	7 cut(s)	401, 675, 724, 1329, 2460, 2540, 2712
HpySE526I	ACGT	3 cut(s)	1372, 1588, 2572
KflI	GGGWCCC	1 cut(s)	1324
LmnI	GCTCC	3 cut(s)	670, 703, 2825
Lsp1109I	GCAGC	5 cut(s)	567, 748, 1719, 2008, 2467
LweI	GCATC	9 cut(s)	550, 594, 782, 1459, 1687, 1820, 1858, 2201, 2770
MaeI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
MaeII	ACGT	3 cut(s)	1372, 1588, 2572
MaeIII	GTNAC	6 cut(s)	576, 648, 1918, 1946, 2468, 2505
MfeI	CAATTG	3 cut(s)	2050, 2371, 2756
MflI	RGATCY	1 cut(s)	2665
MhlI	GDGCHC	5 cut(s)	667, 882, 1372, 2752, 2841
MlsI	TGGCCA	2 cut(s)	1896, 2419
MluNI	TGGCCA	2 cut(s)	1896, 2419
MlyI	GAGTC	6 cut(s)	206, 920, 1085, 1132, 2309, 2476
MmeI	TCCRAC	2 cut(s)	62, 2391
Mox20I	TGGCCA	2 cut(s)	1896, 2419
Mph1103I	ATGCAT	1 cut(s)	1813
MroXI	GAANNNNTTC	2 cut(s)	444, 1230
MscI	TGGCCA	2 cut(s)	1896, 2419
MseI	TTAA	7 cut(s)	474, 750, 1401, 1592, 1851, 2030, 2139
MslI	CAYNNNNRTG	6 cut(s)	1449, 1455, 2160, 2277, 2475, 2615
Msp20I	TGGCCA	2 cut(s)	1896, 2419
MspA1I	CMGCKG	1 cut(s)	1097
MspCI	CTTAAG	1 cut(s)	2029
MspI	CCGG	3 cut(s)	581, 1645, 2293
MspR9I	CCNGG	6 cut(s)	236, 295, 582, 1500, 2604, 2649
MunI	CAATTG	3 cut(s)	2050, 2371, 2756
Mva1269I	GAATGC	1 cut(s)	266
MvaI	CCWGG	5 cut(s)	236, 295, 1500, 2604, 2649
MvnI	CGCG	2 cut(s)	1062, 2313
MwoI	GCNNNNNNNGC	4 cut(s)	521, 1349, 1697, 2826
NciI	CCSGG	1 cut(s)	582
NdeI	CATATG	5 cut(s)	607, 868, 1665, 2092, 2113
NheI	GCTAGC	1 cut(s)	1565
NlaIV	GGNNCC	8 cut(s)	616, 666, 705, 1325, 1326, 2667, 2750, 2821
NmeAIII	GCCGAG	1 cut(s)	161
NmuCI	GTSAC	3 cut(s)	648, 1918, 2468
NruI	TCGCGA	1 cut(s)	2313
NsiI	ATGCAT	1 cut(s)	1813
NspI	RCATGY	3 cut(s)	1551, 1984, 2829
OliI	CACNNNNGTG	1 cut(s)	2277
PaeI	GCATGC	2 cut(s)	1984, 2829
PagI	TCATGA	3 cut(s)	2155, 2616, 2635
PciI	ACATGT	1 cut(s)	1547
PctI	GAATGC	1 cut(s)	266
PdmI	GAANNNNTTC	2 cut(s)	444, 1230
PfeI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 2287
PflMI	CCANNNNNTGG	2 cut(s)	713, 2373
PkrI	GCNGC	6 cut(s)	349, 557, 738, 1709, 2023, 2482
PleI	GAGTC	6 cut(s)	206, 920, 1085, 1132, 2309, 2475
PmaCI	CACGTG	1 cut(s)	1373
PmlI	CACGTG	1 cut(s)	1373
PpsI	GAGTC	6 cut(s)	206, 920, 1085, 1132, 2309, 2475
Ppu21I	YACGTR	1 cut(s)	1373
PpuMI	RGGWCCY	2 cut(s)	614, 1324
PscI	ACATGT	1 cut(s)	1547
PshAI	GACNNNNGTC	1 cut(s)	1142
PsiI	TTATAA	2 cut(s)	1293, 1629
Psp1406I	AACGTT	1 cut(s)	1588
Psp5II	RGGWCCY	2 cut(s)	614, 1324
Psp6I	CCWGG	5 cut(s)	234, 293, 1498, 2602, 2647
PspCI	CACGTG	1 cut(s)	1373
PspFI	CCCAGC	1 cut(s)	204
PspGI	CCWGG	5 cut(s)	234, 293, 1498, 2602, 2647
PspN4I	GGNNCC	8 cut(s)	616, 666, 705, 1325, 1326, 2667, 2750, 2821
PspOMI	GGGCCC	1 cut(s)	2748
PspPI	GGNCC	8 cut(s)	614, 773, 1324, 1460, 1958, 2588, 2748, 2749
PspPPI	RGGWCCY	2 cut(s)	614, 1324
PstI	CTGCAG	2 cut(s)	576, 1709
PstNI	CAGNNNCTG	3 cut(s)	686, 1967, 2603
PsuI	RGATCY	1 cut(s)	2665
RruI	TCGCGA	1 cut(s)	2313
RsaI	GTAC	7 cut(s)	766, 1304, 1505, 1546, 1834, 2519, 2769
RsaNI	GTAC	7 cut(s)	765, 1303, 1504, 1545, 1833, 2518, 2768
RseI	CAYNNNNRTG	6 cut(s)	1449, 1455, 2160, 2277, 2475, 2615
SaqAI	TTAA	7 cut(s)	474, 750, 1401, 1592, 1851, 2030, 2139
SatI	GCNGC	6 cut(s)	348, 556, 737, 1708, 2022, 2481
Sau96I	GGNCC	8 cut(s)	614, 773, 1324, 1460, 1958, 2588, 2748, 2749
ScaI	AGTACT	1 cut(s)	766
SchI	GAGTC	6 cut(s)	206, 920, 1085, 1132, 2309, 2476
ScrFI	CCNGG	6 cut(s)	236, 295, 582, 1500, 2604, 2649
SduI	GDGCHC	5 cut(s)	667, 882, 1372, 2752, 2841
SfaNI	GCATC	9 cut(s)	550, 594, 782, 1459, 1687, 1820, 1858, 2201, 2770
SfcI	CTRYAG	4 cut(s)	463, 572, 1705, 2484
SinI	GGWCC	3 cut(s)	614, 773, 1324
SmiMI	CAYNNNNRTG	6 cut(s)	1449, 1455, 2160, 2277, 2475, 2615
SmlI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SmoI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SphI	GCATGC	2 cut(s)	1984, 2829
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
StyD4I	CCNGG	6 cut(s)	234, 293, 580, 1498, 2602, 2647
StyI	CCWWGG	1 cut(s)	619
TaaI	ACNGT	6 cut(s)	43, 1045, 1367, 1544, 2137, 2836
TaiI	ACGT	3 cut(s)	1375, 1591, 2575
TaqI	TCGA	5 cut(s)	167, 1485, 1858, 2349, 2865
TatI	WGTACW	3 cut(s)	764, 1544, 2767
TauI	GCSGC	1 cut(s)	350
TfiI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 2287
Tru1I	TTAA	7 cut(s)	474, 750, 1401, 1592, 1851, 2030, 2139
Tru9I	TTAA	7 cut(s)	474, 750, 1401, 1592, 1851, 2030, 2139
TscAI	CASTG	9 cut(s)	283, 633, 714, 1033, 1050, 1528, 1567, 1619, 2279
TseFI	GTSAC	3 cut(s)	648, 1918, 2468
TseI	GCWGC	5 cut(s)	555, 736, 1707, 2021, 2480
Tsp45I	GTSAC	3 cut(s)	648, 1918, 2468
TspGWI	ACGGA	2 cut(s)	2188, 2525
TspRI	CASTG	9 cut(s)	283, 633, 714, 1033, 1050, 1528, 1567, 1619, 2279
Van91I	CCANNNNNTGG	2 cut(s)	713, 2373
Vha464I	CTTAAG	1 cut(s)	2029
VneI	GTGCAC	1 cut(s)	1368
VpaK11BI	GGWCC	3 cut(s)	614, 773, 1324
XagI	CCTNNNNNAGG	1 cut(s)	1715
XapI	RAATTY	6 cut(s)	1055, 1229, 1474, 2193, 2221, 2491
XceI	RCATGY	3 cut(s)	1551, 1984, 2829
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1283
XmiI	GTMKAC	4 cut(s)	819, 1437, 1927, 2260
XmnI	GAANNNNTTC	2 cut(s)	444, 1230
XspI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
ZrmI	AGTACT	1 cut(s)	766
Zsp2I	ATGCAT	1 cut(s)	1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.