Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

55702631 .. 55713950

Gene Structure 11320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG285800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGCCTTTATGTACTTGTTCTCCGCCTCTTCCTCCTCCAACGGTCAACAGTCATTCCCTTCCTCACCGCCGCGCCTATTCTTCATCCTCCATACGCCGTCGTTTCACCTCCCAAGCCAAACCCATTTTCTCCTTCCCGCCAAATTCCGCCAAGTTTTCCACAACACCGCTACTTCAATGCTCGTCCTCATTGCCCATGACTGCCGAACAGGACTCCTCTTCCGCCTCACATCCCCAGGTGCAGGGAAGCTTGTTGAAATCAAGGGCATTCTGGGTTAGTGAATCAATCATTGCCTGGAATGTGGATGCTGGAAATGATTCGTGCTACTTATTCGCTAGTGGAACTGCGGCGTTGTCATTAACGGGTGATGGAGTTTTGGGTGAGGATGTAAAAGTTCAGCTTCAAGAGGATAGACATGGGCTGCCGGAAAATGTGAAAACAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAGGGTCCCCCCTGATCTGGATGTCAGACCTCTTCTCAAAGCTCAGTTGGCAGTTGCTACTTTCAGTTCTGATGGAAGGTGCAGCGATGCTACTGGTCTGCAGTTACCGGGTGTTCTAGATGAATTGTTCTCGTATGATGGTTCCCTTGGTGCAGTGTATTCAAAAGAAGCTGTGTCCCTTTACCTTTGGGCACCTACTTCTCAGGCAGTATCTGTATTGATCTATAAGGAGCCATCGGGTGGAAATCCCTTAGAAATTGTGCAGCTTGAGGAAGTTAATGGTATTTGGAGTACTAGAGGACCAAAAACTTGGGAAGGATGCTACTATGTGTATGAAGTGTCTGTATACCACCCTAGCACCTTACAAATTGAAAAGTGCTATGCAAATGATCCATATGCTAGAGGGCTCTCAGCAGATGGTAGGCGGACATTGTTCATCAATCTTGATTCTGATGATATAAAACCAGAAGGATGGGATAAATTGGTAGATGAGAAACCTGATATACATTCTTCTTCGGACATAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGTGGTGGATATCTGGCATTCACTTCGCAGGACTCGGCTGGCGTATCTCATTTGAAGAAATTATCAAATGCTGGTATCACTCATGTCCATCTACTGCCAGCCTACCAATTTGCAGGTGTTGATGATGAGAAGGGGAACTGGAAGAGTGTAGATTCCAAGATTCTTGAAAAGTTTCCTTCAGATTCATCCGAGCAACAAGCTCTAATTACAGCAATCCAAAATGATGATGGGTACAACTGGGGGTACAACCCTGTCCTTTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTTCATACCGGGCAATTGAGTTTAGGAAGATGGTTCAGGCACTTAATCGTTTTGGCCTTCGTGTTGTCTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGAAAATTCAGTTCTTGATAAGATTGTTCCAGGTTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAATAGCACATGTGTAAACAACACTGCTAGCGAGCATTTCATGGTAGAGCATTTAATTCTTGATGATCTTTTGCACTGGGCAGTTGATTTTAAGGTTGATGGATTCCGGTTTGACCTTATGGGTCATATAATGAAAAGAACAATGGTGAAAGCAAAAGATGCGCTTTGCAGCCTAACAAAGGATAGGGATGGAGTTGATGGCTCAAGTATCTATATATATGGTGAAGGATGGGATTTTGGTGAAGTGGCCAACAATGGGCGTGGAGTGAATGCATCGCAGTTCAATATTTGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTGGGTGGTTCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCAGAAGTTGTTGCAGAACGTATGCTTGCCGAATCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTGAGGGACTTTGTGCTAACCAATTATGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGTGGGACAAGTGTAGCATATGCTTTGGACCCTACTGAAACTGTAAGGATCTCAATTAGACTTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

720

Amino Acids

79.31

Weight (kDa)

5.48

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	86 - 199	2e-33	Pullulanase N2 domain
CBM_48	PF02922	207 - 291	1.2e-06	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1163
Acc36I	ACCTGC	1 cut(s)	1163
AccB1I	GGYRCC	1 cut(s)	664
AccB7I	CCANNNNNTGG	1 cut(s)	713
AccI	GTMKAC	3 cut(s)	819, 1437, 1927
AccII	CGCG	1 cut(s)	72
AciI	CCGC	9 cut(s)	23, 67, 70, 137, 147, 167, 222, 347, 898
AclWI	GGATC	4 cut(s)	857, 2007, 2083, 2150
AcoI	YGGCCR	2 cut(s)	1785, 1894
AcsI	RAATTY	3 cut(s)	142, 1055, 1474
AcuI	CTGAAG	2 cut(s)	1221, 1889
AfaI	GTAC	5 cut(s)	13, 766, 1292, 1304, 1834
AfiI	CCNNNNNNNGG	6 cut(s)	241, 490, 713, 1321, 1717, 1794
AflIII	ACRYGT	1 cut(s)	1547
AgsI	TTSAA	8 cut(s)	176, 256, 404, 636, 845, 1114, 1226, 1822
AjnI	CCWGG	3 cut(s)	234, 293, 1498
AjuI	GAANNNNNNNTTGG	2 cut(s)	110, 142
AluBI	AGCT	7 cut(s)	249, 400, 515, 644, 739, 1259, 1338
AluI	AGCT	7 cut(s)	249, 400, 515, 644, 739, 1259, 1338
AlwI	GGATC	4 cut(s)	857, 2007, 2083, 2150
AlwNI	CAGNNNCTG	1 cut(s)	686
AoxI	GGCC	5 cut(s)	1411, 1460, 1785, 1894, 1957
ApeKI	GCWGC	5 cut(s)	421, 555, 736, 1707, 2021
ApoI	RAATTY	3 cut(s)	142, 1055, 1474
Asp700I	GAANNNNTTC	2 cut(s)	316, 1230
AspLEI	GCGC	2 cut(s)	74, 1702
AspS9I	GGNCC	6 cut(s)	478, 773, 1324, 1460, 1958, 2122
AsuC2I	CCSGG	2 cut(s)	582, 1367
AsuHPI	GGTGA	7 cut(s)	56, 97, 377, 392, 1696, 1772, 1790
AsuNHI	GCTAGC	1 cut(s)	1565
AvaII	GGWCC	4 cut(s)	478, 773, 1324, 2122
BaeGI	GKGCMC	1 cut(s)	667
BalI	TGGCCA	2 cut(s)	1787, 1896
BanI	GGYRCC	1 cut(s)	664
BanII	GRGCYC	1 cut(s)	882
BbvI	GCAGC	5 cut(s)	408, 567, 748, 1719, 2008
BceAI	ACGGC	1 cut(s)	81
BcgI	CGANNNNNNTGC	2 cut(s)	1065, 1099
BciT130I	CCWGG	3 cut(s)	236, 295, 1500
BcnI	CCSGG	2 cut(s)	582, 1367
BfaI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
BfmI	CTRYAG	2 cut(s)	463, 572
BfuAI	ACCTGC	1 cut(s)	1163
BisI	GCNGC	7 cut(s)	70, 348, 422, 556, 737, 1708, 2022
BlsI	GCNGC	7 cut(s)	71, 349, 423, 557, 738, 1709, 2023
BmcAI	AGTACT	1 cut(s)	766
Bme1390I	CCNGG	5 cut(s)	236, 295, 582, 1367, 1500
Bme18I	GGWCC	4 cut(s)	478, 773, 1324, 2122
BmgT120I	GGNCC	6 cut(s)	478, 773, 1324, 1460, 1958, 2122
BmiI	GGNNCC	8 cut(s)	479, 480, 616, 666, 705, 1325, 1326, 2124
BmrFI	CCNGG	5 cut(s)	236, 295, 582, 1367, 1500
BmrI	ACTGGG	3 cut(s)	1306, 1624, 1886
BmsI	GCATC	6 cut(s)	295, 550, 782, 1687, 1820, 1858
BmtI	GCTAGC	1 cut(s)	1569
BmuI	ACTGGG	3 cut(s)	1306, 1624, 1886
BpuEI	CTTGAG	4 cut(s)	761, 1531, 1726, 2050
BpuMI	CCSGG	2 cut(s)	582, 1367
BsaJI	CCNNGG	2 cut(s)	234, 619
BsaWI	WCCGGW	1 cut(s)	1644
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	6 cut(s)	241, 490, 713, 1321, 1717, 1794
Bse1I	ACTGG	7 cut(s)	571, 1202, 1301, 1619, 1840, 1881, 1927
Bse3DI	GCAATG	2 cut(s)	188, 288
BseBI	CCWGG	3 cut(s)	236, 295, 1500
BseDI	CCNNGG	2 cut(s)	234, 619
BseLI	CCNNNNNNNGG	6 cut(s)	241, 490, 713, 1321, 1717, 1794
BseMI	GCAATG	2 cut(s)	188, 288
BseMII	CTCAG	3 cut(s)	530, 689, 897
BseNI	ACTGG	7 cut(s)	571, 1202, 1301, 1619, 1840, 1881, 1927
BseRI	GAGGAG	2 cut(s)	24, 205
BseSI	GKGCMC	1 cut(s)	667
BseXI	GCAGC	5 cut(s)	408, 567, 748, 1719, 2008
BsgI	GTGCAG	4 cut(s)	260, 574, 645, 755
Bsh1236I	CGCG	1 cut(s)	72
BshFI	GGCC	5 cut(s)	1413, 1462, 1787, 1896, 1959
BshNI	GGYRCC	1 cut(s)	664
BsiSI	CCGG	4 cut(s)	425, 581, 1366, 1645
BslFI	GGGAC	5 cut(s)	464, 634, 1310, 2048, 2113
BslI	CCNNNNNNNGG	6 cut(s)	241, 490, 713, 1321, 1717, 1794
BsmFI	GGGAC	5 cut(s)	464, 634, 1310, 2048, 2113
BsmI	GAATGC	3 cut(s)	266, 1076, 1813
BsnI	GGCC	5 cut(s)	1413, 1462, 1787, 1896, 1959
Bsp1286I	GDGCHC	2 cut(s)	667, 882
Bsp143I	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
BspACI	CCGC	9 cut(s)	23, 67, 70, 137, 147, 167, 222, 347, 898
BspANI	GGCC	5 cut(s)	1413, 1462, 1787, 1896, 1959
BspCNI	CTCAG	3 cut(s)	529, 688, 896
BspFNI	CGCG	1 cut(s)	72
BspLI	GGNNCC	8 cut(s)	479, 480, 616, 666, 705, 1325, 1326, 2124
BspMAI	CTGCAG	1 cut(s)	576
BspMI	ACCTGC	1 cut(s)	1163
BspOI	GCTAGC	1 cut(s)	1569
BspPI	GGATC	4 cut(s)	857, 2007, 2083, 2150
BspT107I	GGYRCC	1 cut(s)	664
BsrDI	GCAATG	2 cut(s)	188, 288
BsrI	ACTGG	7 cut(s)	571, 1202, 1301, 1619, 1840, 1881, 1927
BssECI	CCNNGG	2 cut(s)	234, 619
BssMI	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
BssNAI	GTATAC	1 cut(s)	820
BssT1I	CCWWGG	1 cut(s)	619
Bst1107I	GTATAC	1 cut(s)	820
Bst2UI	CCWGG	3 cut(s)	236, 295, 1500
Bst4CI	ACNGT	4 cut(s)	43, 50, 1045, 2137
Bst6I	CTCTTC	5 cut(s)	33, 223, 510, 1196, 1908
BstAPI	GCANNNNNTGC	2 cut(s)	1349, 1697
BstC8I	GCNNGC	5 cut(s)	1099, 1158, 1567, 1571, 1986
BstDEI	CTNAG	4 cut(s)	516, 675, 724, 883
BstENI	CCTNNNNNAGG	1 cut(s)	1715
BstFNI	CGCG	1 cut(s)	72
BstHHI	GCGC	2 cut(s)	74, 1702
BstKTI	GATC	9 cut(s)	490, 696, 865, 1606, 1858, 1954, 2002, 2078, 2145
BstMBI	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
BstMWI	GCNNNNNNNGC	3 cut(s)	521, 1349, 1697
BstNI	CCWGG	3 cut(s)	236, 295, 1500
BstNSI	RCATGY	1 cut(s)	1551
BstSCI	CCNGG	5 cut(s)	234, 293, 580, 1365, 1498
BstSFI	CTRYAG	2 cut(s)	463, 572
BstSLI	GKGCMC	1 cut(s)	667
BstUI	CGCG	1 cut(s)	72
BstV1I	GCAGC	5 cut(s)	408, 567, 748, 1719, 2008
BstX2I	RGATCY	1 cut(s)	2142
BstXI	CCANNNNNNTGG	2 cut(s)	783, 945
BstYI	RGATCY	1 cut(s)	2142
BstZ17I	GTATAC	1 cut(s)	820
BsuRI	GGCC	5 cut(s)	1413, 1462, 1787, 1896, 1959
BtgZI	GCGATG	2 cut(s)	573, 1797
BtsI	GCAGTG	2 cut(s)	633, 1560
BtsIMutI	CAGTG	6 cut(s)	633, 1033, 1050, 1521, 1560, 1612
BveI	ACCTGC	1 cut(s)	1163
Cac8I	GCNNGC	5 cut(s)	1099, 1158, 1567, 1571, 1986
CaiI	CAGNNNCTG	1 cut(s)	686
CfoI	GCGC	2 cut(s)	74, 1702
Cfr13I	GGNCC	6 cut(s)	478, 773, 1324, 1460, 1958, 2122
Csp6I	GTAC	5 cut(s)	12, 765, 1291, 1303, 1833
CspCI	CAANNNNNGTGG	4 cut(s)	1780, 1810, 1815, 1845
CviAII	CATG	7 cut(s)	196, 416, 1142, 1451, 1548, 1579, 1955
CviQI	GTAC	5 cut(s)	12, 765, 1291, 1303, 1833
DdeI	CTNAG	4 cut(s)	516, 675, 724, 883
DpnI	GATC	9 cut(s)	489, 695, 864, 1605, 1857, 1953, 2001, 2077, 2144
DpnII	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
EaeI	YGGCCR	2 cut(s)	1785, 1894
Eam1104I	CTCTTC	5 cut(s)	33, 223, 510, 1196, 1908
EarI	CTCTTC	5 cut(s)	33, 223, 510, 1196, 1908
EciI	GGCGGA	4 cut(s)	12, 136, 211, 913
Eco130I	CCWWGG	1 cut(s)	619
Eco24I	GRGCYC	1 cut(s)	882
Eco32I	GATATC	1 cut(s)	1070
Eco47I	GGWCC	4 cut(s)	478, 773, 1324, 2122
Eco57I	CTGAAG	2 cut(s)	1221, 1889
EcoNI	CCTNNNNNAGG	1 cut(s)	1715
EcoO109I	RGGNCCY	2 cut(s)	478, 1324
EcoRII	CCWGG	3 cut(s)	234, 293, 1498
EcoRV	GATATC	1 cut(s)	1070
EcoT14I	CCWWGG	1 cut(s)	619
EcoT22I	ATGCAT	1 cut(s)	1813
EcoT38I	GRGCYC	1 cut(s)	882
ErhI	CCWWGG	1 cut(s)	619
FaeI	CATG	7 cut(s)	199, 419, 1145, 1454, 1551, 1582, 1958
FalI	AAGNNNNNCTT	4 cut(s)	384, 416, 1686, 1718
FaqI	GGGAC	5 cut(s)	464, 634, 1310, 2048, 2113
FatI	CATG	7 cut(s)	195, 415, 1141, 1450, 1547, 1578, 1954
FauI	CCCGC	1 cut(s)	144
FauNDI	CATATG	3 cut(s)	868, 2092, 2113
FblI	GTMKAC	3 cut(s)	819, 1437, 1927
Fnu4HI	GCNGC	7 cut(s)	70, 348, 422, 556, 737, 1708, 2022
FriOI	GRGCYC	1 cut(s)	882
Fsp4HI	GCNGC	7 cut(s)	70, 348, 422, 556, 737, 1708, 2022
FspBI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
GlaI	GCGC	2 cut(s)	73, 1701
GluI	GCNGC	7 cut(s)	70, 348, 422, 556, 737, 1708, 2022
HaeIII	GGCC	5 cut(s)	1413, 1462, 1787, 1896, 1959
HapII	CCGG	4 cut(s)	425, 581, 1366, 1645
HhaI	GCGC	2 cut(s)	74, 1702
Hin1II	CATG	7 cut(s)	199, 419, 1145, 1454, 1551, 1582, 1958
Hin6I	GCGC	2 cut(s)	72, 1700
HinP1I	GCGC	2 cut(s)	72, 1700
HincII	GTYRAC	1 cut(s)	46
HindII	GTYRAC	1 cut(s)	46
HindIII	AAGCTT	1 cut(s)	247
HpaII	CCGG	4 cut(s)	425, 581, 1366, 1645
HphI	GGTGA	7 cut(s)	56, 97, 377, 392, 1696, 1772, 1790
Hpy166II	GTNNAC	5 cut(s)	46, 820, 1438, 1555, 1928
Hpy188I	TCNGA	7 cut(s)	500, 544, 925, 991, 1240, 1249, 2080
Hpy188III	TCNNGA	8 cut(s)	404, 491, 590, 917, 1052, 1223, 1484, 1598
Hpy8I	GTNNAC	5 cut(s)	46, 820, 1438, 1555, 1928
Hpy99I	CGWCG	1 cut(s)	102
HpyCH4III	ACNGT	4 cut(s)	43, 50, 1045, 2137
HpyCH4IV	ACGT	1 cut(s)	1978
HpyF10VI	GCNNNNNNNGC	3 cut(s)	521, 1349, 1697
HpyF3I	CTNAG	4 cut(s)	516, 675, 724, 883
HpySE526I	ACGT	1 cut(s)	1978
Hsp92II	CATG	7 cut(s)	199, 419, 1145, 1454, 1551, 1582, 1958
HspAI	GCGC	2 cut(s)	72, 1700
KflI	GGGWCCC	2 cut(s)	478, 1324
Kzo9I	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
LmnI	GCTCC	1 cut(s)	703
Lsp1109I	GCAGC	5 cut(s)	408, 567, 748, 1719, 2008
LweI	GCATC	6 cut(s)	295, 550, 782, 1687, 1820, 1858
MaeI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
MaeII	ACGT	1 cut(s)	1978
MaeIII	GTNAC	4 cut(s)	576, 1502, 1918, 1946
MalI	GATC	9 cut(s)	489, 695, 864, 1605, 1857, 1953, 2001, 2077, 2144
MboI	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
MfeI	CAATTG	1 cut(s)	1371
MflI	RGATCY	1 cut(s)	2142
MhlI	GDGCHC	2 cut(s)	667, 882
MlsI	TGGCCA	2 cut(s)	1787, 1896
MluNI	TGGCCA	2 cut(s)	1787, 1896
MlyI	GAGTC	2 cut(s)	206, 1085
MmeI	TCCRAC	1 cut(s)	62
Mox20I	TGGCCA	2 cut(s)	1787, 1896
Mph1103I	ATGCAT	1 cut(s)	1813
MroXI	GAANNNNTTC	2 cut(s)	316, 1230
MscI	TGGCCA	2 cut(s)	1787, 1896
MseI	TTAA	7 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2161
MslI	CAYNNNNRTG	2 cut(s)	1449, 1455
Msp20I	TGGCCA	2 cut(s)	1787, 1896
MspI	CCGG	4 cut(s)	425, 581, 1366, 1645
MspR9I	CCNGG	5 cut(s)	236, 295, 582, 1367, 1500
MunI	CAATTG	1 cut(s)	1371
Mva1269I	GAATGC	3 cut(s)	266, 1076, 1813
MvaI	CCWGG	3 cut(s)	236, 295, 1500
MvnI	CGCG	1 cut(s)	72
MwoI	GCNNNNNNNGC	3 cut(s)	521, 1349, 1697
NciI	CCSGG	2 cut(s)	582, 1367
NdeI	CATATG	3 cut(s)	868, 2092, 2113
NdeII	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
NheI	GCTAGC	1 cut(s)	1565
NlaIII	CATG	7 cut(s)	199, 419, 1145, 1454, 1551, 1582, 1958
NlaIV	GGNNCC	8 cut(s)	479, 480, 616, 666, 705, 1325, 1326, 2124
NmeAIII	GCCGAG	1 cut(s)	1073
NmuCI	GTSAC	1 cut(s)	1918
NsiI	ATGCAT	1 cut(s)	1813
NspI	RCATGY	1 cut(s)	1551
PaqCI	CACCTGC	1 cut(s)	1163
PciI	ACATGT	1 cut(s)	1547
PctI	GAATGC	3 cut(s)	266, 1076, 1813
PdmI	GAANNNNTTC	2 cut(s)	316, 1230
PfeI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 1991
PflMI	CCANNNNNTGG	1 cut(s)	713
PkrI	GCNGC	7 cut(s)	71, 349, 423, 557, 738, 1709, 2023
PleI	GAGTC	2 cut(s)	206, 1085
PpsI	GAGTC	2 cut(s)	206, 1085
PpuMI	RGGWCCY	2 cut(s)	478, 1324
PscI	ACATGT	1 cut(s)	1547
Psp5II	RGGWCCY	2 cut(s)	478, 1324
Psp6I	CCWGG	3 cut(s)	234, 293, 1498
PspGI	CCWGG	3 cut(s)	234, 293, 1498
PspN4I	GGNNCC	8 cut(s)	479, 480, 616, 666, 705, 1325, 1326, 2124
PspPI	GGNCC	6 cut(s)	478, 773, 1324, 1460, 1958, 2122
PspPPI	RGGWCCY	2 cut(s)	478, 1324
PstI	CTGCAG	1 cut(s)	576
PstNI	CAGNNNCTG	1 cut(s)	686
PsuI	RGATCY	1 cut(s)	2142
RsaI	GTAC	5 cut(s)	13, 766, 1292, 1304, 1834
RsaNI	GTAC	5 cut(s)	12, 765, 1291, 1303, 1833
RseI	CAYNNNNRTG	2 cut(s)	1449, 1455
SaqAI	TTAA	7 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2161
SatI	GCNGC	7 cut(s)	70, 348, 422, 556, 737, 1708, 2022
Sau3AI	GATC	9 cut(s)	487, 693, 862, 1603, 1855, 1951, 1999, 2075, 2142
Sau96I	GGNCC	6 cut(s)	478, 773, 1324, 1460, 1958, 2122
ScaI	AGTACT	1 cut(s)	766
SchI	GAGTC	2 cut(s)	206, 1085
ScrFI	CCNGG	5 cut(s)	236, 295, 582, 1367, 1500
SduI	GDGCHC	2 cut(s)	667, 882
SfaNI	GCATC	6 cut(s)	295, 550, 782, 1687, 1820, 1858
SfcI	CTRYAG	2 cut(s)	463, 572
SinI	GGWCC	4 cut(s)	478, 773, 1324, 2122
SmiMI	CAYNNNNRTG	2 cut(s)	1449, 1455
SmlI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SmoI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SsiI	CCGC	9 cut(s)	23, 67, 70, 137, 147, 167, 222, 347, 898
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
StyD4I	CCNGG	5 cut(s)	234, 293, 580, 1365, 1498
StyI	CCWWGG	1 cut(s)	619
TaaI	ACNGT	4 cut(s)	43, 50, 1045, 2137
TaiI	ACGT	1 cut(s)	1981
TaqI	TCGA	1 cut(s)	1858
TatI	WGTACW	2 cut(s)	11, 764
TauI	GCSGC	2 cut(s)	72, 350
TfiI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 1991
Tru1I	TTAA	7 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2161
Tru9I	TTAA	7 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2161
TscAI	CASTG	6 cut(s)	633, 1033, 1050, 1528, 1567, 1619
TseFI	GTSAC	1 cut(s)	1918
TseI	GCWGC	5 cut(s)	421, 555, 736, 1707, 2021
Tsp45I	GTSAC	1 cut(s)	1918
TspRI	CASTG	6 cut(s)	633, 1033, 1050, 1528, 1567, 1619
Van91I	CCANNNNNTGG	1 cut(s)	713
VpaK11BI	GGWCC	4 cut(s)	478, 773, 1324, 2122
XagI	CCTNNNNNAGG	1 cut(s)	1715
XapI	RAATTY	3 cut(s)	142, 1055, 1474
XbaI	TCTAGA	1 cut(s)	589
XceI	RCATGY	1 cut(s)	1551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1283
XmiI	GTMKAC	3 cut(s)	819, 1437, 1927
XmnI	GAANNNNTTC	2 cut(s)	316, 1230
XspI	CTAG	6 cut(s)	336, 590, 768, 828, 873, 1566
ZrmI	AGTACT	1 cut(s)	766
Zsp2I	ATGCAT	1 cut(s)	1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.