Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Forward (+)

13776945 .. 13792521

Gene Structure 15577 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027669

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2820 bp

ATGCCTTTATGTACTTGTTCTCCACTTCTTCCTCCTCCAACGGTCAACAGTCATTCCCCTCCTCACCGCCGCGCCTATTCTTCATCCTCCATACGCCGTCGTTTCACCTCCCAAGCCAAACCCATTTTCTCCTTCCCGCCAAATTCCGCCAAGTTTTCCAGAACACCGCTACTTCAATGCTCGTCCTCATTGCCCATGACTGCCGAACAAGACTCCTCTTCAACCTCACAGCCTCAGGTGCAGGGAAGCTTGTTGAAATCAAGGGCTTTCTGGGTTAGTGAATCAATCATTGCCTGGAATGTGGATGCTGGAAATGATTCGTGCTACTTATTCGCTAGTGAAACTGCGGCGTTGTCATTAACGGTTGATGGAGTTTTGGGTGAGGATGTAAAAGTTCAGCTTCAAGAGGATAGACATGGGCTACCGGAAAATGTGAAAGCAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAGGGTCCCCCCTGATCTGGATGTCAAACCTCTTCTCAAAGCTCAGTTGGCGGTTGCTACTTTCAGTTCTGATGGAAGGTGCAGTGATGCTACTGGTCTGCAGTTACCGGGCGTCCTAGATGAATTGTTCTCGTATGATGGTTCCCTTGGTGCAGTGTATTCAAAAGAAGCTGTGTCCCTTTACCTTTGGGCACCTACTTCTCAGGCAGTATCTGTATTGATCTATAAGGAGCCATCGGGTGGAAATCCCTTAGAAATTGTGCAGCTTGAGGAAGTTAATGGCGTTTGGAGTACAAGAGGACCAAAAACTTGGGAAGGATGCTACTATGTGTATGAAGTGTCTGTATACCACCCTAGCACCTTACAAATTGAAAAGTGCTATGCAAATGATCCATATGCTAGAGGGCTTTCAGCAGATGGTTGGCGGACATTGTTCATCAATCTTGATTCTGATCATATAAAACCAGACGGATGGGATAAATTGGTAGATGAGAAACCTGATATACATTCTTCTTCGGACATAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGCAGTGGATATCTGGCATTCACTTCGCAGGACTCGGCCGGTGTATCTCATTTGAAGAAATTATCAAATGCTGGTATCACTCATGTTCATCTACTGCCAGCCCACCAATTTGCAGGTGTTGATGATGAGAAGGGGAACTGGAAGAGTGTAGATTCCAAGATTCTTGAAAAGTTTCCTTCAGATTCATCTGAGCAACAAGCTCTAATTACAGCTATCCAAAATGATGATGGGTATAACTGGGGGTACAACCCTGTCCTTTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTTCATACCGGGCAATTGAGTTTAGGAAGATGGTTCAGGCACTTAATCGTTTTGGCCTTCGTGTTGTCTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGAAAATTCAGTTCTTGATAAGATTGTTCCAGGTTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAATAGCACATGTGTAAACAACACTGCTAGCGAGCATTTTATGGTAGAGCGTTTAATTCTTGATGATCTTTTGCACTGGGCAGTTGATTTTAAGGTTGATGGATTCCGGTTTGACCTTATGGGTCATATAATGAAAAGAACAATGGTGAAAGCAAAAGATGCACTTTGCAGCCTAACAAAGGATAGGGATGGAGTTGATGGCTCAAGTATCTATATATATGGTGAAGGATGGGATTTTGGTGAAGTGGCCAACAATGGGCGTGGAGTAAATGCATCGCAGTTCAATATTTGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTGGGTGGTTCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCAGAAGTTGTTGCAGAACGTATGCTTGCTGAATCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTGAGAGACTTTGTGCTAACCAATTATGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGTGGGACAAGTGTAGCATATGCTTTGGACCCTACTGAAACTGTTAATTATGCTTCAGCTCATGACAACGAAACTCTTTTTGACATCGTGAGTCTGAAGGGAATTCCATTTTTCCACTCCGGCGATGAGATGCTCCGGTCCAAATCACTTGATCGCGACTCATACAATTCTGGTGATTGGTTCAACAGGATTGACTTCACTTATAATTCCAACAATTGGGGTGTTGGCCTTCCTCCAAAAGAGAAAAATGAAAAGAGCTGGCCACTAATGAAACCAAGATTAGCAGAGCCATCCTTTAAGCCTCAGAAGAGTCACATCCATGCTGCTGTGCAGAATTTTTCAGATTTGTTACGTATTAGGTACTCTTCACCACTTTTCCGTCTGAGGACAGCAAATGCTATTCAGGAACGCGTACGTTTCCACAATACTGGGCCTGCATCAGTCCCTGGCGTCATAGTAATGAGCATTGAAGATGGTCATGAAGGACTACCAGGGTTATCTCAACTGGATCCAATCTACTCCTACATTGTGGTTATCGTCAATGCACGCCCAACTGAGATGTCATTTACAAGTTCTTCTATTCGTGCTAGGACCCTTCAATTGCATCCTGTACAGGTTAAGTCAAATGATGAAATAGTGAAGAGATCTACTTATGACATAGGCTCGGGATGCTTTACTGTGCCCGCAAGGACGACATCTGTGTTTGTCGAGCCTCGGGGGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

940

Amino Acids

104.01

Weight (kDa)

5.81

Isoelectric Point (pI)

40.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	86 - 199	6.6e-34	Pullulanase N2 domain
CBM_48	PF02922	207 - 291	8.7e-07	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	766 - 935	4.2e-66	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2298
AarI	CACCTGC	1 cut(s)	1163
Acc36I	ACCTGC	1 cut(s)	1163
AccB1I	GGYRCC	1 cut(s)	664
AccB7I	CCANNNNNTGG	2 cut(s)	713, 2310
AccI	GTMKAC	3 cut(s)	819, 1437, 1927
AccII	CGCG	3 cut(s)	72, 2250, 2505
AciI	CCGC	9 cut(s)	67, 70, 137, 147, 167, 347, 524, 898, 2778
AclWI	GGATC	5 cut(s)	857, 2007, 2083, 2597, 2610
AcoI	YGGCCR	4 cut(s)	1095, 1785, 1894, 2354
AcsI	RAATTY	5 cut(s)	142, 1055, 1474, 2196, 2428
AcuI	CTGAAG	4 cut(s)	1221, 1889, 2133, 2210
AcyI	GRCGYC	2 cut(s)	585, 2544
AfaI	GTAC	7 cut(s)	13, 766, 1304, 1834, 2456, 2508, 2706
AfiI	CCNNNNNNNGG	6 cut(s)	490, 713, 1321, 1717, 1794, 2310
AflIII	ACRYGT	2 cut(s)	1547, 2503
AjnI	CCWGG	4 cut(s)	293, 1498, 2539, 2584
AjuI	GAANNNNNNNTTGG	2 cut(s)	110, 142
Alw26I	GTCTC	1 cut(s)	2028
AlwI	GGATC	5 cut(s)	857, 2007, 2083, 2597, 2610
AlwNI	CAGNNNCTG	2 cut(s)	686, 2540
Ama87I	CYCGRG	2 cut(s)	2758, 2808
AoxI	GGCC	9 cut(s)	1095, 1411, 1460, 1785, 1894, 1957, 2320, 2354, 2525
ApeKI	GCWGC	4 cut(s)	736, 1707, 2021, 2417
ApoI	RAATTY	5 cut(s)	142, 1055, 1474, 2196, 2428
Asp700I	GAANNNNTTC	2 cut(s)	316, 1230
AspLEI	GCGC	1 cut(s)	74
AspS9I	GGNCC	9 cut(s)	478, 773, 1324, 1460, 1958, 2122, 2232, 2525, 2685
AsuC2I	CCSGG	2 cut(s)	582, 1367
AsuHPI	GGTGA	8 cut(s)	56, 97, 392, 1696, 1772, 1790, 2279, 2454
AsuNHI	GCTAGC	1 cut(s)	1565
AvaI	CYCGRG	2 cut(s)	2758, 2808
AvaII	GGWCC	6 cut(s)	478, 773, 1324, 2122, 2232, 2685
AxyI	CCTNAGG	1 cut(s)	234
BaeGI	GKGCMC	2 cut(s)	667, 2778
BalI	TGGCCA	3 cut(s)	1787, 1896, 2356
BamHI	GGATCC	1 cut(s)	2602
BanI	GGYRCC	1 cut(s)	664
BbvI	GCAGC	4 cut(s)	748, 1719, 2008, 2404
BceAI	ACGGC	1 cut(s)	81
BcgI	CGANNNNNNTGC	2 cut(s)	1065, 1099
BciT130I	CCWGG	4 cut(s)	295, 1500, 2541, 2586
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	2 cut(s)	582, 1367
BcoDI	GTCTC	1 cut(s)	2028
BfaI	CTAG	6 cut(s)	336, 590, 828, 873, 1566, 2682
BfmI	CTRYAG	2 cut(s)	463, 572
BfuAI	ACCTGC	1 cut(s)	1163
BglII	AGATCT	1 cut(s)	2738
BisI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2418
BlsI	GCNGC	6 cut(s)	71, 349, 738, 1709, 2023, 2419
Bme1390I	CCNGG	6 cut(s)	295, 582, 1367, 1500, 2541, 2586
Bme18I	GGWCC	6 cut(s)	478, 773, 1324, 2122, 2232, 2685
BmeT110I	CYCGRG	2 cut(s)	2758, 2808
BmgT120I	GGNCC	9 cut(s)	478, 773, 1324, 1460, 1958, 2122, 2232, 2525, 2685
BmrFI	CCNGG	6 cut(s)	295, 582, 1367, 1500, 2541, 2586
BmrI	ACTGGG	4 cut(s)	1306, 1624, 1886, 2532
BmtI	GCTAGC	1 cut(s)	1569
BmuI	ACTGGG	4 cut(s)	1306, 1624, 1886, 2532
BpuEI	CTTGAG	4 cut(s)	761, 1531, 1726, 2050
BpuMI	CCSGG	2 cut(s)	582, 1367
BsaAI	YACGTR	1 cut(s)	2447
BsaBI	GATNNNNATC	1 cut(s)	924
BsaHI	GRCGYC	2 cut(s)	585, 2544
BsaJI	CCNNGG	4 cut(s)	619, 2539, 2585, 2807
BsaWI	WCCGGW	3 cut(s)	424, 1644, 2229
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	6 cut(s)	490, 713, 1321, 1717, 1794, 2310
Bse118I	RCCGGY	1 cut(s)	1097
Bse1I	ACTGG	9 cut(s)	571, 1202, 1301, 1619, 1840, 1881, 1927, 2527, 2604
Bse21I	CCTNAGG	1 cut(s)	234
Bse3DI	GCAATG	2 cut(s)	188, 288
Bse8I	GATNNNNATC	1 cut(s)	924
BseBI	CCWGG	4 cut(s)	295, 1500, 2541, 2586
BseDI	CCNNGG	4 cut(s)	619, 2539, 2585, 2807
BseJI	GATNNNNATC	1 cut(s)	924
BseLI	CCNNNNNNNGG	6 cut(s)	490, 713, 1321, 1717, 1794, 2310
BseMI	GCAATG	2 cut(s)	188, 288
BseMII	CTCAG	7 cut(s)	248, 530, 689, 1239, 2411, 2468, 2640
BseNI	ACTGG	9 cut(s)	571, 1202, 1301, 1619, 1840, 1881, 1927, 2527, 2604
BseRI	GAGGAG	3 cut(s)	24, 51, 205
BseSI	GKGCMC	2 cut(s)	667, 2778
BseX3I	CGGCCG	1 cut(s)	1095
BseXI	GCAGC	4 cut(s)	748, 1719, 2008, 2404
BsgI	GTGCAG	5 cut(s)	260, 574, 645, 755, 2444
Bsh1236I	CGCG	3 cut(s)	72, 2250, 2505
Bsh1285I	CGRYCG	1 cut(s)	1098
BshFI	GGCC	9 cut(s)	1097, 1413, 1462, 1787, 1896, 1959, 2322, 2356, 2527
BshNI	GGYRCC	1 cut(s)	664
BsiEI	CGRYCG	1 cut(s)	1098
BsiHKCI	CYCGRG	2 cut(s)	2758, 2808
BsiSI	CCGG	7 cut(s)	425, 581, 1098, 1366, 1645, 2214, 2230
BsiWI	CGTACG	1 cut(s)	2506
BslFI	GGGAC	5 cut(s)	464, 634, 1310, 2113, 2522
BslI	CCNNNNNNNGG	6 cut(s)	490, 713, 1321, 1717, 1794, 2310
BsmAI	GTCTC	1 cut(s)	2028
BsmFI	GGGAC	5 cut(s)	464, 634, 1310, 2113, 2522
BsmI	GAATGC	1 cut(s)	1076
BsnI	GGCC	9 cut(s)	1097, 1413, 1462, 1787, 1896, 1959, 2322, 2356, 2527
BsoBI	CYCGRG	2 cut(s)	2758, 2808
Bsp1286I	GDGCHC	2 cut(s)	667, 2778
Bsp1407I	TGTACA	1 cut(s)	2704
Bsp68I	TCGCGA	1 cut(s)	2250
BspACI	CCGC	9 cut(s)	67, 70, 137, 147, 167, 347, 524, 898, 2778
BspANI	GGCC	9 cut(s)	1097, 1413, 1462, 1787, 1896, 1959, 2322, 2356, 2527
BspCNI	CTCAG	7 cut(s)	247, 529, 688, 1240, 2410, 2469, 2641
BspFNI	CGCG	3 cut(s)	72, 2250, 2505
BspHI	TCATGA	2 cut(s)	2155, 2572
BspMAI	CTGCAG	1 cut(s)	576
BspMI	ACCTGC	1 cut(s)	1163
BspOI	GCTAGC	1 cut(s)	1569
BspPI	GGATC	5 cut(s)	857, 2007, 2083, 2597, 2610
BspT107I	GGYRCC	1 cut(s)	664
BsrDI	GCAATG	2 cut(s)	188, 288
BsrFI	RCCGGY	1 cut(s)	1097
BsrGI	TGTACA	1 cut(s)	2704
BsrI	ACTGG	9 cut(s)	571, 1202, 1301, 1619, 1840, 1881, 1927, 2527, 2604
BssAI	RCCGGY	1 cut(s)	1097
BssECI	CCNNGG	4 cut(s)	619, 2539, 2585, 2807
BssNAI	GTATAC	1 cut(s)	820
BssNI	GRCGYC	2 cut(s)	585, 2544
BssT1I	CCWWGG	1 cut(s)	619
Bst1107I	GTATAC	1 cut(s)	820
Bst2UI	CCWGG	4 cut(s)	295, 1500, 2541, 2586
Bst4CI	ACNGT	6 cut(s)	43, 50, 364, 1045, 2137, 2773
Bst6I	CTCTTC	7 cut(s)	223, 510, 1196, 1908, 2396, 2464, 2729
BstACI	GRCGYC	2 cut(s)	585, 2544
BstAPI	GCANNNNNTGC	2 cut(s)	1349, 1697
BstAUI	TGTACA	1 cut(s)	2704
BstBAI	YACGTR	1 cut(s)	2447
BstC8I	GCNNGC	8 cut(s)	1158, 1567, 1571, 1986, 2354, 2529, 2641, 2778
BstDEI	CTNAG	8 cut(s)	234, 516, 675, 724, 1248, 2397, 2477, 2649
BstENI	CCTNNNNNAGG	1 cut(s)	1715
BstFNI	CGCG	3 cut(s)	72, 2250, 2505
BstHHI	GCGC	1 cut(s)	74
BstMAI	GTCTC	1 cut(s)	2028
BstMCI	CGRYCG	1 cut(s)	1098
BstMWI	GCNNNNNNNGC	5 cut(s)	238, 521, 1349, 1697, 2763
BstNI	CCWGG	4 cut(s)	295, 1500, 2541, 2586
BstNSI	RCATGY	1 cut(s)	1551
BstSCI	CCNGG	6 cut(s)	293, 580, 1365, 1498, 2539, 2584
BstSFI	CTRYAG	2 cut(s)	463, 572
BstSLI	GKGCMC	2 cut(s)	667, 2778
BstSNI	TACGTA	1 cut(s)	2447
BstUI	CGCG	3 cut(s)	72, 2250, 2505
BstV1I	GCAGC	4 cut(s)	748, 1719, 2008, 2404
BstX2I	RGATCY	2 cut(s)	2602, 2738
BstXI	CCANNNNNNTGG	3 cut(s)	783, 945, 2522
BstYI	RGATCY	2 cut(s)	2602, 2738
BstZ17I	GTATAC	1 cut(s)	820
BstZI	CGGCCG	1 cut(s)	1095
Bsu36I	CCTNAGG	1 cut(s)	234
BsuRI	GGCC	9 cut(s)	1097, 1413, 1462, 1787, 1896, 1959, 2322, 2356, 2527
BtgZI	GCGATG	2 cut(s)	1797, 2232
BtsI	GCAGTG	4 cut(s)	562, 633, 1069, 1560
BtsIMutI	CAGTG	8 cut(s)	562, 633, 1033, 1050, 1069, 1521, 1560, 1612
BtuMI	TCGCGA	1 cut(s)	2250
BveI	ACCTGC	1 cut(s)	1163
Cac8I	GCNNGC	8 cut(s)	1158, 1567, 1571, 1986, 2354, 2529, 2641, 2778
CaiI	CAGNNNCTG	2 cut(s)	686, 2540
CciI	TCATGA	2 cut(s)	2155, 2572
CfoI	GCGC	1 cut(s)	74
Cfr10I	RCCGGY	1 cut(s)	1097
Cfr13I	GGNCC	9 cut(s)	478, 773, 1324, 1460, 1958, 2122, 2232, 2525, 2685
CseI	GACGC	2 cut(s)	574, 2533
Csp6I	GTAC	7 cut(s)	12, 765, 1303, 1833, 2455, 2507, 2705
CspCI	CAANNNNNGTGG	4 cut(s)	1780, 1810, 1815, 1845
CviAII	CATG	9 cut(s)	196, 416, 1142, 1451, 1548, 1955, 2156, 2414, 2573
CviQI	GTAC	7 cut(s)	12, 765, 1303, 1833, 2455, 2507, 2705
DdeI	CTNAG	8 cut(s)	234, 516, 675, 724, 1248, 2397, 2477, 2649
EaeI	YGGCCR	4 cut(s)	1095, 1785, 1894, 2354
EagI	CGGCCG	1 cut(s)	1095
Eam1104I	CTCTTC	7 cut(s)	223, 510, 1196, 1908, 2396, 2464, 2729
EarI	CTCTTC	7 cut(s)	223, 510, 1196, 1908, 2396, 2464, 2729
EciI	GGCGGA	2 cut(s)	136, 913
EclXI	CGGCCG	1 cut(s)	1095
Eco105I	TACGTA	1 cut(s)	2447
Eco130I	CCWWGG	1 cut(s)	619
Eco32I	GATATC	1 cut(s)	1070
Eco47I	GGWCC	6 cut(s)	478, 773, 1324, 2122, 2232, 2685
Eco52I	CGGCCG	1 cut(s)	1095
Eco57I	CTGAAG	4 cut(s)	1221, 1889, 2133, 2210
Eco81I	CCTNAGG	1 cut(s)	234
Eco88I	CYCGRG	2 cut(s)	2758, 2808
EcoNI	CCTNNNNNAGG	1 cut(s)	1715
EcoO109I	RGGNCCY	3 cut(s)	478, 1324, 2685
EcoRI	GAATTC	1 cut(s)	2196
EcoRII	CCWGG	4 cut(s)	293, 1498, 2539, 2584
EcoRV	GATATC	1 cut(s)	1070
EcoT14I	CCWWGG	1 cut(s)	619
EcoT22I	ATGCAT	1 cut(s)	1813
ErhI	CCWWGG	1 cut(s)	619
FaeI	CATG	9 cut(s)	199, 419, 1145, 1454, 1551, 1958, 2159, 2417, 2576
FalI	AAGNNNNNCTT	4 cut(s)	384, 416, 1686, 1718
FaqI	GGGAC	5 cut(s)	464, 634, 1310, 2113, 2522
FatI	CATG	9 cut(s)	195, 415, 1141, 1450, 1547, 1954, 2155, 2413, 2572
FauI	CCCGC	2 cut(s)	144, 2785
FauNDI	CATATG	3 cut(s)	868, 2092, 2113
FbaI	TGATCA	1 cut(s)	925
FblI	GTMKAC	3 cut(s)	819, 1437, 1927
Fnu4HI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2418
Fsp4HI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2418
FspBI	CTAG	6 cut(s)	336, 590, 828, 873, 1566, 2682
GlaI	GCGC	1 cut(s)	73
GluI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2418
HaeIII	GGCC	9 cut(s)	1097, 1413, 1462, 1787, 1896, 1959, 2322, 2356, 2527
HapII	CCGG	7 cut(s)	425, 581, 1098, 1366, 1645, 2214, 2230
HgaI	GACGC	2 cut(s)	574, 2533
HhaI	GCGC	1 cut(s)	74
Hin1I	GRCGYC	2 cut(s)	585, 2544
Hin1II	CATG	9 cut(s)	199, 419, 1145, 1454, 1551, 1958, 2159, 2417, 2576
Hin6I	GCGC	1 cut(s)	72
HinP1I	GCGC	1 cut(s)	72
HincII	GTYRAC	1 cut(s)	46
HindII	GTYRAC	1 cut(s)	46
HindIII	AAGCTT	1 cut(s)	247
HpaII	CCGG	7 cut(s)	425, 581, 1098, 1366, 1645, 2214, 2230
HphI	GGTGA	8 cut(s)	56, 97, 392, 1696, 1772, 1790, 2279, 2454
Hpy166II	GTNNAC	5 cut(s)	46, 820, 1438, 1555, 1928
Hpy8I	GTNNAC	5 cut(s)	46, 820, 1438, 1555, 1928
Hpy99I	CGWCG	1 cut(s)	102
HpyCH4III	ACNGT	6 cut(s)	43, 50, 364, 1045, 2137, 2773
HpyCH4IV	ACGT	3 cut(s)	1978, 2446, 2509
HpyF10VI	GCNNNNNNNGC	5 cut(s)	238, 521, 1349, 1697, 2763
HpyF3I	CTNAG	8 cut(s)	234, 516, 675, 724, 1248, 2397, 2477, 2649
HpySE526I	ACGT	3 cut(s)	1978, 2446, 2509
Hsp92I	GRCGYC	2 cut(s)	585, 2544
Hsp92II	CATG	9 cut(s)	199, 419, 1145, 1454, 1551, 1958, 2159, 2417, 2576
HspAI	GCGC	1 cut(s)	72
KflI	GGGWCCC	2 cut(s)	478, 1324
Ksp22I	TGATCA	1 cut(s)	925
LmnI	GCTCC	2 cut(s)	703, 2232
Lsp1109I	GCAGC	4 cut(s)	748, 1719, 2008, 2404
MaeI	CTAG	6 cut(s)	336, 590, 828, 873, 1566, 2682
MaeII	ACGT	3 cut(s)	1978, 2446, 2509
MaeIII	GTNAC	6 cut(s)	576, 1502, 1918, 1946, 2405, 2442
MfeI	CAATTG	3 cut(s)	1371, 2308, 2693
MflI	RGATCY	2 cut(s)	2602, 2738
MhlI	GDGCHC	2 cut(s)	667, 2778
MlsI	TGGCCA	3 cut(s)	1787, 1896, 2356
MluI	ACGCGT	1 cut(s)	2503
MluNI	TGGCCA	3 cut(s)	1787, 1896, 2356
MlyI	GAGTC	5 cut(s)	206, 1085, 2194, 2246, 2413
MmeI	TCCRAC	2 cut(s)	62, 2328
Mox20I	TGGCCA	3 cut(s)	1787, 1896, 2356
Mph1103I	ATGCAT	1 cut(s)	1813
MroXI	GAANNNNTTC	2 cut(s)	316, 1230
MscI	TGGCCA	3 cut(s)	1787, 1896, 2356
MseI	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2391, 2712
MslI	CAYNNNNRTG	4 cut(s)	1449, 1455, 2412, 2552
Msp20I	TGGCCA	3 cut(s)	1787, 1896, 2356
MspI	CCGG	7 cut(s)	425, 581, 1098, 1366, 1645, 2214, 2230
MspR9I	CCNGG	6 cut(s)	295, 582, 1367, 1500, 2541, 2586
MunI	CAATTG	3 cut(s)	1371, 2308, 2693
Mva1269I	GAATGC	1 cut(s)	1076
MvaI	CCWGG	4 cut(s)	295, 1500, 2541, 2586
MvnI	CGCG	3 cut(s)	72, 2250, 2505
MwoI	GCNNNNNNNGC	5 cut(s)	238, 521, 1349, 1697, 2763
NciI	CCSGG	2 cut(s)	582, 1367
NdeI	CATATG	3 cut(s)	868, 2092, 2113
NheI	GCTAGC	1 cut(s)	1565
NlaIII	CATG	9 cut(s)	199, 419, 1145, 1454, 1551, 1958, 2159, 2417, 2576
NmeAIII	GCCGAG	1 cut(s)	1073
NmuCI	GTSAC	2 cut(s)	1918, 2405
NruI	TCGCGA	1 cut(s)	2250
NsiI	ATGCAT	1 cut(s)	1813
NspI	RCATGY	1 cut(s)	1551
PagI	TCATGA	2 cut(s)	2155, 2572
PaqCI	CACCTGC	1 cut(s)	1163
PciI	ACATGT	1 cut(s)	1547
PctI	GAATGC	1 cut(s)	1076
PdmI	GAANNNNTTC	2 cut(s)	316, 1230
PfeI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 1991
Pfl23II	CGTACG	1 cut(s)	2506
PflMI	CCANNNNNTGG	2 cut(s)	713, 2310
PkrI	GCNGC	6 cut(s)	71, 349, 738, 1709, 2023, 2419
PleI	GAGTC	5 cut(s)	206, 1085, 2193, 2246, 2412
PpsI	GAGTC	5 cut(s)	206, 1085, 2193, 2246, 2412
Ppu21I	YACGTR	1 cut(s)	2447
PpuMI	RGGWCCY	3 cut(s)	478, 1324, 2685
PscI	ACATGT	1 cut(s)	1547
PsiI	TTATAA	1 cut(s)	2298
Psp5II	RGGWCCY	3 cut(s)	478, 1324, 2685
Psp6I	CCWGG	4 cut(s)	293, 1498, 2539, 2584
PspGI	CCWGG	4 cut(s)	293, 1498, 2539, 2584
PspLI	CGTACG	1 cut(s)	2506
PspPI	GGNCC	9 cut(s)	478, 773, 1324, 1460, 1958, 2122, 2232, 2525, 2685
PspPPI	RGGWCCY	3 cut(s)	478, 1324, 2685
PstI	CTGCAG	1 cut(s)	576
PstNI	CAGNNNCTG	2 cut(s)	686, 2540
PsuI	RGATCY	2 cut(s)	2602, 2738
RruI	TCGCGA	1 cut(s)	2250
RsaI	GTAC	7 cut(s)	13, 766, 1304, 1834, 2456, 2508, 2706
RsaNI	GTAC	7 cut(s)	12, 765, 1303, 1833, 2455, 2507, 2705
RseI	CAYNNNNRTG	4 cut(s)	1449, 1455, 2412, 2552
SaqAI	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2391, 2712
SatI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2418
Sau96I	GGNCC	9 cut(s)	478, 773, 1324, 1460, 1958, 2122, 2232, 2525, 2685
SchI	GAGTC	5 cut(s)	206, 1085, 2194, 2246, 2413
ScrFI	CCNGG	6 cut(s)	295, 582, 1367, 1500, 2541, 2586
SduI	GDGCHC	2 cut(s)	667, 2778
SfcI	CTRYAG	2 cut(s)	463, 572
SinI	GGWCC	6 cut(s)	478, 773, 1324, 2122, 2232, 2685
SmiMI	CAYNNNNRTG	4 cut(s)	1449, 1455, 2412, 2552
SmlI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SmoI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SnaBI	TACGTA	1 cut(s)	2447
SsiI	CCGC	9 cut(s)	67, 70, 137, 147, 167, 347, 524, 898, 2778
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	6 cut(s)	336, 590, 828, 873, 1566, 2682
StyD4I	CCNGG	6 cut(s)	293, 580, 1365, 1498, 2539, 2584
StyI	CCWWGG	1 cut(s)	619
TaaI	ACNGT	6 cut(s)	43, 50, 364, 1045, 2137, 2773
TaiI	ACGT	3 cut(s)	1981, 2449, 2512
TaqI	TCGA	2 cut(s)	1858, 2802
TatI	WGTACW	3 cut(s)	11, 764, 2704
TauI	GCSGC	2 cut(s)	72, 350
TfiI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 1991
Tru1I	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2391, 2712
Tru9I	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2391, 2712
TscAI	CASTG	8 cut(s)	562, 633, 1033, 1050, 1069, 1528, 1567, 1619
TseFI	GTSAC	2 cut(s)	1918, 2405
TseI	GCWGC	4 cut(s)	736, 1707, 2021, 2417
Tsp45I	GTSAC	2 cut(s)	1918, 2405
TspGWI	ACGGA	2 cut(s)	957, 2462
TspRI	CASTG	8 cut(s)	562, 633, 1033, 1050, 1069, 1528, 1567, 1619
Van91I	CCANNNNNTGG	2 cut(s)	713, 2310
VpaK11BI	GGWCC	6 cut(s)	478, 773, 1324, 2122, 2232, 2685
XagI	CCTNNNNNAGG	1 cut(s)	1715
XapI	RAATTY	5 cut(s)	142, 1055, 1474, 2196, 2428
XceI	RCATGY	1 cut(s)	1551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1283
XmiI	GTMKAC	3 cut(s)	819, 1437, 1927
XmnI	GAANNNNTTC	2 cut(s)	316, 1230
XspI	CTAG	6 cut(s)	336, 590, 828, 873, 1566, 2682
Zsp2I	ATGCAT	1 cut(s)	1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.