Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

21318351 .. 21327798

Gene Structure 9448 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1100100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2940 bp

ATGCCTTTATTATATTCGTCTACGTTTCTTCTTAACCGGCCACCATCAACGGCTCCAACGACCAACCGCCACTTCCCTCCTCAACGCCCCGCCACGTGTCCTTCATCCTCTCTACGGCGCCGTTTCGCCTCCGCGCGTAAACCCCTTCTCCCGCCGCTCCCACTCCGCTCCGCCGGGGTTTTCAGACACCCCAATTTGCAGTGCTCGTCGTCGTCGTCGTCGTCCGTATCAATGTCGGCCGAGGAGGAGCCCACTTCGACCTCGCAGAGGGGTGAACAGCTGCAGAGTGGCTTCTTGTATTCGAGGGCGTTCTGGGTTAGTGAGTCAATTATTGCCTGGAATGTCGATGTTGGAAATGGTTCCTGCTACTTATTTGTTAGTAAAACCGCGGCTTTATCTTGTTCGAGTGATGGAATTCTAGGTGAGGATATAAAAGTAAAGCTTGAAGAAGATAAACATGGGCTTCCGGAAAATGTGAAAGAAAAATTTCCACATATCAAAGATTACAGAGCTTTTAATGTGCCCCCTGATTTGGATGCCAAACCTCTTCTCAAATGCCAACTGGAAGTTGCTACTTTCGATGCTAATGGAAGGTGCAGTGATGCCACTGGTCTGCAGTTACCTGGTATTTTAGATGAATTGTTCTCATATAATGGTCCCCTTGGTGCACTTTATTCAGAAGAATCAGTGTCACTGTACCTTTGGGCGCCTACGGCTCAGGAAGTATGTGTTTGCATCTATAAGGAACCGTTAGGTGGGGGGAATCCCCAAGAAGTTGTGCAACTTGAGGAGGTTAATGGCGTTTGGAGTACTAAAGGGCCAAAAAGTTGGGAAGGCTGCTATTATGTGTATGAAGTGTCTGTATATCATCCTAGCACCTTGAAAATTGAAAAGTGCTATGCAAACGATCCATATGCTAGAGGGCTTTCATCAGATGGCAGGCGGACATTGCTGGTTAATCTTGATTCTGATGATATAAAACCTGAAGGATGGGATAAATTGGCTTATGAGAAGCCTGATATAATTTCTTTCTCTGACATAAGTATTTATGAGTTACACATCAGAGATTTCAGTGCCAATGACCAGACTGTGCATTCTGAATTTCGTGGTGGATATCTTGCCTTCACTTTGCAGGATTCAGCGGGTGCATTTCATTTGAAGAAATTATCAAATGCTGGTATCACTCATGTCCATCTACTGCCAGCCTTCCAATTTGCAGGGGTTGATGATGAAAATGAGAACTGGAAGAATGTAGATTTCAAGATACTTGAAAAGTTTGCTCCAGATTCGGATGAGCAACAAGCTCTTATTACAGCTATCCAAAATGATGATGGATATAACTGGGGGTACAACCCTGTTCTCTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTACATACCGTGCAATTGAGTTCAGGAAGATGGTCCAGGCGCTTAACCGTTACGGCCTTCGTGTTGTCTTGGATGCTGTCTACAATCATTTGTCCGAAAGTGGGCCATTTAGTGTGAATTCTGTTCTTGATAAGATTGTTCCAGGATACTACTTAAGAAGAAATACAGATGGCTTTATCGAGAATAGTACATGTGTGAACAACACTGCTAGTGAGCATTTTATGGTCGAGCGTTTAATTGTTGATGATCTATTGCACTGGGCAGTTGATTACAAGGTTGATGGGTTCCGGTTCGATCTTATGGGTCATATAATGAGAAGAACAATGGTGAAAGCAAAAGATGCACTCTGCAGCCTAACAAAGGAAAGGGATGGAGTTGATGGCTCAAGTATCTATATATATGGCGAAGGATGGGATTTTGGTGAAGTTGCCAATAATGGACGTGGAATAAATGCGTCACAGTCCAATCTTCGTGGAACTGGAATTGGAAGTTTTAATGATCGAATACGGGATGCAATACTTGGTGGATCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACCGGTCTACTCTTGCAGCCTAATGGTCACGATCATGGTCCAGAAAGTGTTGCAAAACACATGCTTGCTGAATCAAAGGATCACATTCAGGTTGGAATGGCTGCAAACCTGAGGGATTTTGTGCTAACTGATTATGAAGGGAAAGAGGTAAAAGGAGCAGAAGTATTGACCTATGGCGGTACACCTGTAGCATATACTTTATCTCCTACTGAAACTATCAATTATGTTTCTGCTCATGACAACGAAACTCTGTTTGACATCGTGGCTCTGAAGACTTCGATGGAAATTTCTTTAGAGGAGAGATGCAGGATAAATCACTTGGCGACAAGTATAATAGCACTCGGACAGGGGATACCATTTTTCCACGCCGGCGATGAGCTGCTTCGGTCAAAATCACTTGATCGTGACTCATACAATTCTGGTGATTGGTTCAATAGGCTGGACTTCACTTATAGCTCTAACAATTGGGGTGTTGGCCTTCCTCCAAAAGAGAAGAACGAACACAGCTGGCCGCTAATGAAACCAAGATTAGCAGATCCATCCTTTAAGCCTCAGAAAAGTCACATCCTCGCTGCTTTGGAGAACTTTTCTAATTTGTTACGTATTAGGTACACTTCACCACTTTTTCGTTTGAGGACGGCAAATGCTATTCAGGAACGGGTACGTTTCCATAATACTGGCCCTTCATTAGTCCCTGGAGTCATAGTAATGAGCATTGAAGATGGTCATGAAGGCGTGCCAGGGTTATCTCAGCTCGATCCAATTTTCTCTTACATTGTGGTTATCATCAATGCGTGTCCAACAGAGGTGTCATTTGCCAGTCCCCCTCTTCAGTCTAGGACTCTTCAGTTGCATCCTGTGCAGGTAACGTCAACGGATGAAATAGTTAAGAGATCTACCTATGATGCTTCCTCAGGATGCTTCACTGTGCCCCCAAGAACTACATCTGTGTTTGTTGAACCTCGGCGAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

980

Amino Acids

108.68

Weight (kDa)

5.66

Isoelectric Point (pI)

42.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	101 - 213	6.8e-32	Pullulanase N2 domain
CBM_48	PF02922	220 - 306	7.2e-08	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	806 - 975	1e-64	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2818
AccB1I	GGYRCC	2 cut(s)	117, 706
AccBSI	CCGCTC	2 cut(s)	157, 168
AccI	GTMKAC	3 cut(s)	20, 1482, 1972
AccII	CGCG	3 cut(s)	134, 136, 389
AccIII	TCCGGA	1 cut(s)	466
AclWI	GGATC	5 cut(s)	902, 1936, 2052, 2495, 2717
AcoI	YGGCCR	4 cut(s)	38, 237, 1939, 2474
AcsI	RAATTY	5 cut(s)	414, 485, 1100, 1519, 2250
AcuI	CTGAAG	5 cut(s)	1005, 1934, 2255, 2780, 2795
AcvI	CACGTG	1 cut(s)	96
AcyI	GRCGYC	2 cut(s)	118, 707
AfaI	GTAC	8 cut(s)	698, 811, 1349, 1405, 1591, 2146, 2576, 2628
AfiI	CCNNNNNNNGG	6 cut(s)	114, 267, 532, 755, 1503, 1762
AflII	CTTAAG	1 cut(s)	1555
AflIII	ACRYGT	2 cut(s)	95, 1592
AgeI	ACCGGT	1 cut(s)	1967
AgsI	TTSAA	9 cut(s)	446, 883, 890, 1159, 1261, 1271, 2398, 2684, 2924
AjiI	CACGTC	1 cut(s)	1844
AjnI	CCWGG	6 cut(s)	335, 622, 1437, 1543, 2659, 2704
AjuI	GAANNNNNNNTTGG	2 cut(s)	56, 88
Alw21I	GWGCWC	2 cut(s)	206, 670
Alw44I	GTGCAC	1 cut(s)	666
AlwI	GGATC	5 cut(s)	902, 1936, 2052, 2495, 2717
Aor13HI	TCCGGA	1 cut(s)	466
AoxI	GGCC	9 cut(s)	38, 237, 818, 1456, 1505, 1939, 2440, 2474, 2644
ApaLI	GTGCAC	1 cut(s)	666
ApeKI	GCWGC	7 cut(s)	280, 837, 1752, 1981, 2066, 2344, 2537
ApoI	RAATTY	5 cut(s)	414, 485, 1100, 1519, 2250
AsiGI	ACCGGT	1 cut(s)	1967
Asp700I	GAANNNNTTC	2 cut(s)	358, 486
AspLEI	GCGC	4 cut(s)	120, 136, 709, 1444
AspS9I	GGNCC	7 cut(s)	656, 818, 1369, 1435, 1505, 2003, 2645
AsuC2I	CCSGG	1 cut(s)	175
AsuHPI	GGTGA	6 cut(s)	284, 434, 1741, 1835, 2399, 2574
AvaII	GGWCC	4 cut(s)	656, 1369, 1435, 2003
AxyI	CCTNAGG	2 cut(s)	2075, 2878
BaeGI	GKGCMC	3 cut(s)	525, 670, 2898
BaeI	ACNNNNGTAYC	2 cut(s)	2136, 2169
BalI	TGGCCA	1 cut(s)	1941
BanI	GGYRCC	2 cut(s)	117, 706
BanII	GRGCYC	1 cut(s)	252
BbrPI	CACGTG	1 cut(s)	96
BbsI	GAAGAC	1 cut(s)	2243
Bbv12I	GWGCWC	2 cut(s)	206, 670
BbvI	GCAGC	7 cut(s)	267, 824, 1764, 1993, 2053, 2331, 2524
BceAI	ACGGC	6 cut(s)	66, 105, 131, 729, 1471, 2619
BcgI	CGANNNNNNTGC	2 cut(s)	896, 930
BciT130I	CCWGG	6 cut(s)	337, 624, 1439, 1545, 2661, 2706
BciVI	GTATCC	2 cut(s)	1541, 2310
BcnI	CCSGG	1 cut(s)	175
BfaI	CTAG	5 cut(s)	419, 873, 918, 1611, 2802
BfmI	CTRYAG	4 cut(s)	281, 614, 1750, 2151
BfoI	RGCGCY	3 cut(s)	121, 710, 1445
BfrI	CTTAAG	1 cut(s)	1555
BfuAI	ACCTGC	1 cut(s)	2818
BfuI	GTATCC	2 cut(s)	1541, 2310
BglII	AGATCT	1 cut(s)	2858
BmcAI	AGTACT	1 cut(s)	811
Bme1390I	CCNGG	7 cut(s)	175, 337, 624, 1439, 1545, 2661, 2706
Bme18I	GGWCC	4 cut(s)	656, 1369, 1435, 2003
BmgBI	CACGTC	1 cut(s)	1844
BmgT120I	GGNCC	7 cut(s)	656, 818, 1369, 1435, 1505, 2003, 2645
BmrFI	CCNGG	7 cut(s)	175, 337, 624, 1439, 1545, 2661, 2706
BmrI	ACTGGG	2 cut(s)	1351, 1669
BmuI	ACTGGG	2 cut(s)	1351, 1669
BpiI	GAAGAC	1 cut(s)	2243
BpmI	CTGGAG	2 cut(s)	1266, 2682
Bpu10I	CCTNAGC	1 cut(s)	717
BpuEI	CTTGAG	2 cut(s)	806, 1771
BpuMI	CCSGG	1 cut(s)	175
BsaAI	YACGTR	2 cut(s)	96, 2567
BsaHI	GRCGYC	2 cut(s)	118, 707
BsaJI	CCNNGG	7 cut(s)	174, 240, 387, 661, 2659, 2705, 2927
BsaWI	WCCGGW	3 cut(s)	466, 1689, 1967
BsaXI	ACNNNNNCTCC	8 cut(s)	132, 162, 2112, 2142, 2152, 2182, 2655, 2685
Bsc4I	CCNNNNNNNGG	6 cut(s)	114, 267, 532, 755, 1503, 1762
Bse118I	RCCGGY	3 cut(s)	36, 1967, 2333
Bse1I	ACTGG	8 cut(s)	567, 613, 1247, 1346, 1664, 1885, 2647, 2784
Bse21I	CCTNAGG	2 cut(s)	2075, 2878
Bse3DI	GCAATG	1 cut(s)	947
BseAI	TCCGGA	1 cut(s)	466
BseBI	CCWGG	6 cut(s)	337, 624, 1439, 1545, 2661, 2706
BseDI	CCNNGG	7 cut(s)	174, 240, 387, 661, 2659, 2705, 2927
BseLI	CCNNNNNNNGG	6 cut(s)	114, 267, 532, 755, 1503, 1762
BseMI	GCAATG	1 cut(s)	947
BseMII	CTCAG	5 cut(s)	731, 2066, 2531, 2729, 2892
BseNI	ACTGG	8 cut(s)	567, 613, 1247, 1346, 1664, 1885, 2647, 2784
BseRI	GAGGAG	5 cut(s)	69, 257, 260, 803, 2276
BseSI	GKGCMC	3 cut(s)	525, 670, 2898
BseX3I	CGGCCG	1 cut(s)	237
BseXI	GCAGC	7 cut(s)	267, 824, 1764, 1993, 2053, 2331, 2524
BsgI	GTGCAG	2 cut(s)	616, 2846
Bsh1236I	CGCG	3 cut(s)	134, 136, 389
Bsh1285I	CGRYCG	1 cut(s)	240
BshFI	GGCC	9 cut(s)	40, 239, 820, 1458, 1507, 1941, 2442, 2476, 2646
BshNI	GGYRCC	2 cut(s)	117, 706
BshTI	ACCGGT	1 cut(s)	1967
BsiEI	CGRYCG	1 cut(s)	240
BsiHKAI	GWGCWC	2 cut(s)	206, 670
BsiSI	CCGG	6 cut(s)	37, 174, 467, 1690, 1968, 2334
BslFI	GGGAC	4 cut(s)	642, 1355, 2642, 2772
BslI	CCNNNNNNNGG	6 cut(s)	114, 267, 532, 755, 1503, 1762
BsmFI	GGGAC	4 cut(s)	642, 1355, 2642, 2772
BsmI	GAATGC	1 cut(s)	1093
BsnI	GGCC	9 cut(s)	40, 239, 820, 1458, 1507, 1941, 2442, 2476, 2646
Bsp1286I	GDGCHC	5 cut(s)	206, 252, 525, 670, 2898
Bsp13I	TCCGGA	1 cut(s)	466
BspANI	GGCC	9 cut(s)	40, 239, 820, 1458, 1507, 1941, 2442, 2476, 2646
BspCNI	CTCAG	5 cut(s)	730, 2067, 2530, 2728, 2891
BspEI	TCCGGA	1 cut(s)	466
BspFNI	CGCG	3 cut(s)	134, 136, 389
BspHI	TCATGA	2 cut(s)	2200, 2692
BspMAI	CTGCAG	3 cut(s)	285, 618, 1754
BspMI	ACCTGC	1 cut(s)	2818
BspPI	GGATC	5 cut(s)	902, 1936, 2052, 2495, 2717
BspT107I	GGYRCC	2 cut(s)	117, 706
BspTI	CTTAAG	1 cut(s)	1555
BsrBI	CCGCTC	2 cut(s)	157, 168
BsrDI	GCAATG	1 cut(s)	947
BsrFI	RCCGGY	3 cut(s)	36, 1967, 2333
BsrI	ACTGG	8 cut(s)	567, 613, 1247, 1346, 1664, 1885, 2647, 2784
BssAI	RCCGGY	3 cut(s)	36, 1967, 2333
BssECI	CCNNGG	7 cut(s)	174, 240, 387, 661, 2659, 2705, 2927
BssNI	GRCGYC	2 cut(s)	118, 707
BssT1I	CCWWGG	1 cut(s)	661
Bst2UI	CCWGG	6 cut(s)	337, 624, 1439, 1545, 2661, 2706
Bst4CI	ACNGT	7 cut(s)	696, 750, 1090, 1412, 1451, 1863, 2893
Bst6I	CTCTTC	4 cut(s)	552, 1953, 2799, 2814
BstACI	GRCGYC	2 cut(s)	118, 707
BstAFI	CTTAAG	1 cut(s)	1555
BstAPI	GCANNNNNTGC	3 cut(s)	1394, 1742, 2824
BstBAI	YACGTR	2 cut(s)	96, 2567
BstC8I	GCNNGC	6 cut(s)	941, 1203, 2031, 2335, 2474, 2702
BstDEI	CTNAG	5 cut(s)	717, 2075, 2517, 2715, 2878
BstDSI	CCRYGG	1 cut(s)	387
BstENI	CCTNNNNNAGG	2 cut(s)	265, 1760
BstFNI	CGCG	3 cut(s)	134, 136, 389
BstH2I	RGCGCY	3 cut(s)	121, 710, 1445
BstHHI	GCGC	4 cut(s)	120, 136, 709, 1444
BstMCI	CGRYCG	1 cut(s)	240
BstMWI	GCNNNNNNNGC	5 cut(s)	713, 949, 1394, 1742, 2824
BstNI	CCWGG	6 cut(s)	337, 624, 1439, 1545, 2661, 2706
BstNSI	RCATGY	2 cut(s)	1596, 2029
BstSCI	CCNGG	7 cut(s)	173, 335, 622, 1437, 1543, 2659, 2704
BstSFI	CTRYAG	4 cut(s)	281, 614, 1750, 2151
BstSLI	GKGCMC	3 cut(s)	525, 670, 2898
BstSNI	TACGTA	1 cut(s)	2567
BstUI	CGCG	3 cut(s)	134, 136, 389
BstV1I	GCAGC	7 cut(s)	267, 824, 1764, 1993, 2053, 2331, 2524
BstV2I	GAAGAC	1 cut(s)	2243
BstX2I	RGATCY	3 cut(s)	1928, 2500, 2858
BstXI	CCANNNNNNTGG	2 cut(s)	828, 2642
BstYI	RGATCY	3 cut(s)	1928, 2500, 2858
BstZI	CGGCCG	1 cut(s)	237
Bsu36I	CCTNAGG	2 cut(s)	2075, 2878
BsuI	GTATCC	2 cut(s)	1541, 2310
BsuRI	GGCC	9 cut(s)	40, 239, 820, 1458, 1507, 1941, 2442, 2476, 2646
BtgI	CCRYGG	1 cut(s)	387
BtgZI	GCGATG	1 cut(s)	2352
BtrI	CACGTC	1 cut(s)	1844
BtsI	GCAGTG	3 cut(s)	206, 604, 1605
BtsIMutI	CAGTG	9 cut(s)	206, 604, 606, 692, 693, 1078, 1605, 1657, 2889
BveI	ACCTGC	1 cut(s)	2818
Cac8I	GCNNGC	6 cut(s)	941, 1203, 2031, 2335, 2474, 2702
CciI	TCATGA	2 cut(s)	2200, 2692
CfoI	GCGC	4 cut(s)	120, 136, 709, 1444
Cfr10I	RCCGGY	3 cut(s)	36, 1967, 2333
Cfr13I	GGNCC	7 cut(s)	656, 818, 1369, 1435, 1505, 2003, 2645
Cfr42I	CCGCGG	1 cut(s)	390
CseI	GACGC	1 cut(s)	1845
CsiI	ACCWGGT	1 cut(s)	622
Csp6I	GTAC	8 cut(s)	697, 810, 1348, 1404, 1590, 2145, 2575, 2627
CspAI	ACCGGT	1 cut(s)	1967
CspCI	CAANNNNNGTGG	2 cut(s)	1855, 1890
CviAII	CATG	7 cut(s)	458, 1187, 1593, 2000, 2026, 2201, 2693
CviQI	GTAC	8 cut(s)	697, 810, 1348, 1404, 1590, 2145, 2575, 2627
DdeI	CTNAG	5 cut(s)	717, 2075, 2517, 2715, 2878
DinI	GGCGCC	2 cut(s)	119, 708
EaeI	YGGCCR	4 cut(s)	38, 237, 1939, 2474
EagI	CGGCCG	1 cut(s)	237
Eam1104I	CTCTTC	4 cut(s)	552, 1953, 2799, 2814
EarI	CTCTTC	4 cut(s)	552, 1953, 2799, 2814
EciI	GGCGGA	2 cut(s)	160, 958
EclXI	CGGCCG	1 cut(s)	237
Eco105I	TACGTA	1 cut(s)	2567
Eco130I	CCWWGG	1 cut(s)	661
Eco24I	GRGCYC	1 cut(s)	252
Eco32I	GATATC	1 cut(s)	1115
Eco47I	GGWCC	4 cut(s)	656, 1369, 1435, 2003
Eco52I	CGGCCG	1 cut(s)	237
Eco57I	CTGAAG	5 cut(s)	1005, 1934, 2255, 2780, 2795
Eco72I	CACGTG	1 cut(s)	96
Eco81I	CCTNAGG	2 cut(s)	2075, 2878
EcoNI	CCTNNNNNAGG	2 cut(s)	265, 1760
EcoO109I	RGGNCCY	1 cut(s)	1369
EcoRI	GAATTC	2 cut(s)	414, 1519
EcoRII	CCWGG	6 cut(s)	335, 622, 1437, 1543, 2659, 2704
EcoRV	GATATC	1 cut(s)	1115
EcoT14I	CCWWGG	1 cut(s)	661
EcoT38I	GRGCYC	1 cut(s)	252
EgeI	GGCGCC	2 cut(s)	119, 708
EheI	GGCGCC	2 cut(s)	119, 708
ErhI	CCWWGG	1 cut(s)	661
FaeI	CATG	7 cut(s)	461, 1190, 1596, 2003, 2029, 2204, 2696
FalI	AAGNNNNNCTT	2 cut(s)	426, 458
FaqI	GGGAC	4 cut(s)	642, 1355, 2642, 2772
FatI	CATG	7 cut(s)	457, 1186, 1592, 1999, 2025, 2200, 2692
FauI	CCCGC	3 cut(s)	97, 159, 1135
FauNDI	CATATG	1 cut(s)	913
FblI	GTMKAC	3 cut(s)	20, 1482, 1972
FriOI	GRGCYC	1 cut(s)	252
FspBI	CTAG	5 cut(s)	419, 873, 918, 1611, 2802
GlaI	GCGC	4 cut(s)	119, 135, 708, 1443
GsuI	CTGGAG	2 cut(s)	1266, 2682
HaeII	RGCGCY	3 cut(s)	121, 710, 1445
HaeIII	GGCC	9 cut(s)	40, 239, 820, 1458, 1507, 1941, 2442, 2476, 2646
HapII	CCGG	6 cut(s)	37, 174, 467, 1690, 1968, 2334
HgaI	GACGC	1 cut(s)	1845
HhaI	GCGC	4 cut(s)	120, 136, 709, 1444
Hin1I	GRCGYC	2 cut(s)	118, 707
Hin1II	CATG	7 cut(s)	461, 1190, 1596, 2003, 2029, 2204, 2696
Hin6I	GCGC	4 cut(s)	118, 134, 707, 1442
HinP1I	GCGC	4 cut(s)	118, 134, 707, 1442
HincII	GTYRAC	1 cut(s)	2838
HindII	GTYRAC	1 cut(s)	2838
HindIII	AAGCTT	1 cut(s)	440
HpaII	CCGG	6 cut(s)	37, 174, 467, 1690, 1968, 2334
HphI	GGTGA	6 cut(s)	284, 434, 1741, 1835, 2399, 2574
Hpy99I	CGWCG	5 cut(s)	211, 214, 217, 220, 223
HpyCH4III	ACNGT	7 cut(s)	696, 750, 1090, 1412, 1451, 1863, 2893
HpyCH4IV	ACGT	6 cut(s)	23, 95, 1843, 2566, 2629, 2834
HpyF10VI	GCNNNNNNNGC	5 cut(s)	713, 949, 1394, 1742, 2824
HpyF3I	CTNAG	5 cut(s)	717, 2075, 2517, 2715, 2878
HpySE526I	ACGT	6 cut(s)	23, 95, 1843, 2566, 2629, 2834
Hsp92I	GRCGYC	2 cut(s)	118, 707
Hsp92II	CATG	7 cut(s)	461, 1190, 1596, 2003, 2029, 2204, 2696
HspAI	GCGC	4 cut(s)	118, 134, 707, 1442
KasI	GGCGCC	2 cut(s)	117, 706
KflI	GGGWCCC	1 cut(s)	1369
Kpn2I	TCCGGA	1 cut(s)	466
KroI	GCCGGC	1 cut(s)	2333
KroNI	GCCGGC	1 cut(s)	2335
KspI	CCGCGG	1 cut(s)	390
LmnI	GCTCC	6 cut(s)	58, 162, 173, 247, 1285, 2120
Lsp1109I	GCAGC	7 cut(s)	267, 824, 1764, 1993, 2053, 2331, 2524
MabI	ACCWGGT	1 cut(s)	622
MaeI	CTAG	5 cut(s)	419, 873, 918, 1611, 2802
MaeII	ACGT	6 cut(s)	23, 95, 1843, 2566, 2629, 2834
MbiI	CCGCTC	2 cut(s)	157, 168
MfeI	CAATTG	2 cut(s)	1416, 2428
MflI	RGATCY	3 cut(s)	1928, 2500, 2858
MhlI	GDGCHC	5 cut(s)	206, 252, 525, 670, 2898
MlsI	TGGCCA	1 cut(s)	1941
MluNI	TGGCCA	1 cut(s)	1941
Mly113I	GGCGCC	2 cut(s)	118, 707
MlyI	GAGTC	4 cut(s)	332, 2366, 2673, 2800
MmeI	TCCRAC	4 cut(s)	80, 331, 2038, 2789
Mox20I	TGGCCA	1 cut(s)	1941
MreI	CGCCGGCG	1 cut(s)	2333
MroI	TCCGGA	1 cut(s)	466
MroNI	GCCGGC	1 cut(s)	2333
MroXI	GAANNNNTTC	2 cut(s)	358, 486
MscI	TGGCCA	1 cut(s)	1941
MslI	CAYNNNNRTG	2 cut(s)	1998, 2672
Msp20I	TGGCCA	1 cut(s)	1941
MspA1I	CMGCKG	4 cut(s)	280, 389, 1142, 2472
MspCI	CTTAAG	1 cut(s)	1555
MspI	CCGG	6 cut(s)	37, 174, 467, 1690, 1968, 2334
MspR9I	CCNGG	7 cut(s)	175, 337, 624, 1439, 1545, 2661, 2706
MunI	CAATTG	2 cut(s)	1416, 2428
Mva1269I	GAATGC	1 cut(s)	1093
MvaI	CCWGG	6 cut(s)	337, 624, 1439, 1545, 2661, 2706
MvnI	CGCG	3 cut(s)	134, 136, 389
MwoI	GCNNNNNNNGC	5 cut(s)	713, 949, 1394, 1742, 2824
NaeI	GCCGGC	1 cut(s)	2335
NarI	GGCGCC	2 cut(s)	118, 707
NciI	CCSGG	1 cut(s)	175
NdeI	CATATG	1 cut(s)	913
NgoMIV	GCCGGC	1 cut(s)	2333
NlaIII	CATG	7 cut(s)	461, 1190, 1596, 2003, 2029, 2204, 2696
NmeAIII	GCCGAG	2 cut(s)	265, 2908
NmuCI	GTSAC	6 cut(s)	690, 1857, 1963, 1991, 2369, 2525
NspI	RCATGY	2 cut(s)	1596, 2029
PagI	TCATGA	2 cut(s)	2200, 2692
PciI	ACATGT	1 cut(s)	1592
PcsI	WCGNNNNNNNCGW	4 cut(s)	56, 212, 215, 218
PctI	GAATGC	1 cut(s)	1093
PdiI	GCCGGC	1 cut(s)	2335
PdmI	GAANNNNTTC	2 cut(s)	358, 486
PfeI	GAWTC	6 cut(s)	683, 763, 965, 1136, 1286, 2036
PfoI	TCCNGGA	1 cut(s)	1543
PinAI	ACCGGT	1 cut(s)	1967
PleI	GAGTC	4 cut(s)	331, 2366, 2672, 2800
PluTI	GGCGCC	2 cut(s)	121, 710
PmaCI	CACGTG	1 cut(s)	96
PmlI	CACGTG	1 cut(s)	96
PpsI	GAGTC	4 cut(s)	331, 2366, 2672, 2800
Ppu21I	YACGTR	2 cut(s)	96, 2567
PpuMI	RGGWCCY	1 cut(s)	1369
PscI	ACATGT	1 cut(s)	1592
Psp5II	RGGWCCY	1 cut(s)	1369
Psp6I	CCWGG	6 cut(s)	335, 622, 1437, 1543, 2659, 2704
PspCI	CACGTG	1 cut(s)	96
PspGI	CCWGG	6 cut(s)	335, 622, 1437, 1543, 2659, 2704
PspPI	GGNCC	7 cut(s)	656, 818, 1369, 1435, 1505, 2003, 2645
PspPPI	RGGWCCY	1 cut(s)	1369
PstI	CTGCAG	3 cut(s)	285, 618, 1754
PsuI	RGATCY	3 cut(s)	1928, 2500, 2858
PvuII	CAGCTG	2 cut(s)	280, 2472
RsaI	GTAC	8 cut(s)	698, 811, 1349, 1405, 1591, 2146, 2576, 2628
RsaNI	GTAC	8 cut(s)	697, 810, 1348, 1404, 1590, 2145, 2575, 2627
RseI	CAYNNNNRTG	2 cut(s)	1998, 2672
SacII	CCGCGG	1 cut(s)	390
Sau96I	GGNCC	7 cut(s)	656, 818, 1369, 1435, 1505, 2003, 2645
ScaI	AGTACT	1 cut(s)	811
SchI	GAGTC	4 cut(s)	332, 2366, 2673, 2800
ScrFI	CCNGG	7 cut(s)	175, 337, 624, 1439, 1545, 2661, 2706
SduI	GDGCHC	5 cut(s)	206, 252, 525, 670, 2898
SexAI	ACCWGGT	1 cut(s)	622
SfcI	CTRYAG	4 cut(s)	281, 614, 1750, 2151
SfoI	GGCGCC	2 cut(s)	119, 708
Sfr303I	CCGCGG	1 cut(s)	390
SgrAI	CRCCGGYG	1 cut(s)	2333
SgrBI	CCGCGG	1 cut(s)	390
SinI	GGWCC	4 cut(s)	656, 1369, 1435, 2003
SmiMI	CAYNNNNRTG	2 cut(s)	1998, 2672
SmlI	CTYRAG	3 cut(s)	785, 1555, 1786
SmoI	CTYRAG	3 cut(s)	785, 1555, 1786
SnaBI	TACGTA	1 cut(s)	2567
SspDI	GGCGCC	2 cut(s)	117, 706
SspMI	CTAG	5 cut(s)	419, 873, 918, 1611, 2802
StyD4I	CCNGG	7 cut(s)	173, 335, 622, 1437, 1543, 2659, 2704
StyI	CCWWGG	1 cut(s)	661
TaaI	ACNGT	7 cut(s)	696, 750, 1090, 1412, 1451, 1863, 2893
TaiI	ACGT	6 cut(s)	26, 98, 1846, 2569, 2632, 2837
TaqII	GACCGA	1 cut(s)	2340
TatI	WGTACW	2 cut(s)	809, 1589
TauI	GCSGC	3 cut(s)	157, 392, 2479
TfiI	GAWTC	6 cut(s)	683, 763, 965, 1136, 1286, 2036
TscAI	CASTG	9 cut(s)	206, 604, 613, 693, 699, 1078, 1612, 1664, 2896
TseFI	GTSAC	6 cut(s)	690, 1857, 1963, 1991, 2369, 2525
TseI	GCWGC	7 cut(s)	280, 837, 1752, 1981, 2066, 2344, 2537
Tsp45I	GTSAC	6 cut(s)	690, 1857, 1963, 1991, 2369, 2525
TspGWI	ACGGA	2 cut(s)	214, 2855
TspRI	CASTG	9 cut(s)	206, 604, 613, 693, 699, 1078, 1612, 1664, 2896
Vha464I	CTTAAG	1 cut(s)	1555
VneI	GTGCAC	1 cut(s)	666
VpaK11BI	GGWCC	4 cut(s)	656, 1369, 1435, 2003
XagI	CCTNNNNNAGG	2 cut(s)	265, 1760
XapI	RAATTY	5 cut(s)	414, 485, 1100, 1519, 2250
XceI	RCATGY	2 cut(s)	1596, 2029
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1328
XmiI	GTMKAC	3 cut(s)	20, 1482, 1972
XmnI	GAANNNNTTC	2 cut(s)	358, 486
XspI	CTAG	5 cut(s)	419, 873, 918, 1611, 2802
ZrmI	AGTACT	1 cut(s)	811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.