Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Forward (+)

14538932 .. 14550132

Gene Structure 11201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00294460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3171 bp

ATGCCTTTATGTGCTTGTTCTCCGCTTCTTCCTCCTCCTCCAACGGTCAACAGTCATTCCCTTCCTCACCGCCGCGCCTATTCTTCATCCTCCATACGCCGTCGTTTCACCTCCCAAGCCAAACCCATTTTCTCCTTCCCGCCAAATTCCGCCAAGTATTCCAGAACACCGCTACTTCAATGCTCGTCCTCATTGCCCATGACTGCCGAACAAGACTCCTCTTCAACCTCACAACCCCAGGTGCAGGGAAGCTTGTTGAAATCAAGGGCATTCTGGGTTAGTCAATCAATCATTGCCTGGAATGTGGATGCTGGAAATGATTCGTGCTACTTATTTGCTAGTGAAACTGCGGCGTTGTCATTAACGGTTGATGGAGTTTTGGGTGAGGATGTAAAAGTTCAGCTTCAAGAGGATAGACATGGGCTACCGGAAAATGTGAAAACAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAGGGTCCCCCTGATCTGGATGTCAGACCTCTTCTCAAAGCTCAGTTGGCAGTTGCTACTTTCAGTTGTAAGGCTTTTGTTGCTCTTAGATTATGATCAATGCGCTGATGGAAGGTGCAGCGATGCTACTGGTCTGCAGTTACCGGGCGTCCTAGATGAATTGTTCTCGTATGATGGTTCCCTTGGTGCAGTGTATTCAAAAGAAGCCGTGTCCCTTTACCTTTGGGCACCTACTTCTCAGGCAGTATCTGTATTGATCTATAAGGAGCCATCGGGTGGAAATCCCTTAGAAATTGTGCAGCTTGAGGAAGTTAATGGCGTTTGGAGTACTAGAGGACCAAAAACTTGGGAAGGATGCTACTATGTGTATGAAGTGTCTGTATACCACCCTAGCACCTTACAAATTGAAAAGTGCTATGCAAATGATCCATATGCTAGAGGGCTTTCAGCGGATGGTAGGCGGACATTGTTCATCAATCTTGATTCTGATCATATAAAACCAGAAGGATGGGATAAATTGGTAGATGAGAAACCTGATATACATTCTTCTTCGGACGTAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGCGGTGGATATCTGGCATTCACTTTGCAGGACTCGGCTGGCGTATCTCATTTGAAGAAATTATCAAATGCTGGTATGACTCATGTCCATCTACTGCCAGCCTACCAATTTGCAGGTGTTGATGATGAGAAGGGGAACTGGAAGAGTGTAGATTCCAAGATTCTTGAAAAGTTTCCTTCAGATTCAGCCGAGCAACAAGCTCTAATTACAGCTATCCAAAATGACGATGGGTATAACTGGGGGTACAACCCTGTCCTTTGGGGGTCCCTAAAGGAAGCTATGCAAGCACTTAATCGTTTTGGCCTTCGTGTTGTCTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGAAAATTCAGTTCTTGATAAGATTGTTCCAGGTTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAATAGCACATGTGTAAACAACACTGCTAGCGAGCATTTTATGGTAGAGCGTTTAATTCTTGATGATCTTTTGCACTGGGCAGTTGATTTTAAGGTTGATGGATTCCGGTTTGACCTTATGGGTCATATAATGAAACGAACAATGGTGAAAGCAAAAGATGCACTTTGCAGCCTAACAAAGGATAGGGATGGAGTTGATGGCTCAAGTATCTATATATATGGTGAAGGATGGGATTTTGGTGAAGTGGCCAACAATGGGCGTGGAGTAAATGCATCGCAGTTCAATATTGGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTGGGTGGTTCTCCGTTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCAGAAGTTGTTGCAGAACGTATGCTTGCTGAATCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTGAGGGACTTTGTGCTAACCAATTATGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGTGGGACAAGTGTAGCATATGCTTTGGACCCTACTGAAACTCTGGTGAGAAATGAAAACATTGAGGATCAGGTTAGAATTAAGGAATTCTTGGAGAATATGGATCTCAGAAGTACTTTTATTAAATCAAGAGTCCCCTATTTAGAAATTGAAAGATACAGAAAGTGCAAGACTCTACCTTACTTGAAAGATGATTGCAACTCTGCAATAATTAAGGTTAATTATGCTTCAGCTCATGACAACGAAACTCTTTTTGACATCGTGAGTCTGAAGACTCCATTTGAAATTTCTGTAGAAGAGAGATGCAGGATAAATCTCTTGGCGTCAAGCATAATAGCACTATCACAGGGAATTCCATTTTTCCACTCTGGCGATGAGATGCTCCGGTCCAAATCACTTGATCGCGACTCATACAATTCTGGTGATTGGTTCAACAGGATTGACTTCACTTATAATTCCAACAATTGGGGTGTTGGCCTTCCTCCAAAAGAGAAAAATGAAAAGAGCTGGCCACTAATGAAACCAAGATTAGCAGAGCCATCCTTTAAGCCTCAGAAGAGTCACATCCATGCTGCTGTGGAGAATTTTTCAGATTTGTTACGTATTAGGTACTCTTCACCACTTTTCCGTCTGAGGACAGCAAATGCTATTCAGGTCGGTACCGAAATTAGAGTAAATCAGGATTCAGGACCCACCATAAAGTGTGCTGCTTCGAATCTGGAACGCGTACGTTTCCACAATACTGGGCCTGCATCAGTCCCTGGTGTCATAGTAATGGGCATTGAAGATGGTGATGAAGGACTACCAGGGTTATCTCAAATGGATCCAATCTACTCCTACATTGTGGTTATCGTCAATGCACGCCCAACTGAGGTGTCATTTACAAGTTCTTCTATTCGTGCTAGGACCCTTCAATTGCATCCTGTACAGGTTAAGTCAAATGATGAAATAGTGAAGAGATCTACTTATGACATAGGCTTGGGATGCTTTACTGTGCCCGCAAGGACAACATCTGTGTTTGTCGAGCCTCGGGGGTGGAGTATGGCTCTTAAATTCTTCTCCAACAAGAGGACTAGGAGTAACACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

1056

Amino Acids

117.88

Weight (kDa)

5.83

Isoelectric Point (pI)

40.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	87 - 212	2.5e-23	Pullulanase N2 domain
CBM_48	PF02922	220 - 304	1e-06	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	845 - 912	2.1e-26	Alpha-1,6-glucosidases, pullulanase-type, C-terminal
Pullul_strch_C	PF11852	935 - 1036	1.6e-29	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2535
AarI	CACCTGC	1 cut(s)	1202
Acc36I	ACCTGC	1 cut(s)	1202
Acc65I	GGTACC	1 cut(s)	2741
AccB1I	GGYRCC	2 cut(s)	703, 2741
AccB7I	CCANNNNNTGG	2 cut(s)	752, 2547
AccI	GTMKAC	3 cut(s)	858, 1425, 1915
AccII	CGCG	4 cut(s)	75, 1101, 2487, 2808
AclWI	GGATC	7 cut(s)	896, 1995, 2071, 2157, 2193, 2900, 2913
AcoI	YGGCCR	3 cut(s)	1773, 1882, 2591
AcsI	RAATTY	8 cut(s)	145, 1094, 1462, 2168, 2367, 2433, 2665, 3134
AcuI	CTGAAG	4 cut(s)	1260, 1877, 2295, 2372
AcyI	GRCGYC	2 cut(s)	624, 2405
AfaI	GTAC	8 cut(s)	805, 1343, 1822, 2197, 2693, 2743, 2811, 3009
AfiI	CCNNNNNNNGG	8 cut(s)	244, 492, 752, 1705, 1782, 2547, 2953, 3150
AflIII	ACRYGT	2 cut(s)	1535, 2806
AjnI	CCWGG	5 cut(s)	237, 296, 1486, 2842, 2887
AjuI	GAANNNNNNNTTGG	2 cut(s)	113, 145
AluBI	AGCT	9 cut(s)	252, 403, 517, 778, 1298, 1310, 1376, 2315, 2589
AluI	AGCT	9 cut(s)	252, 403, 517, 778, 1298, 1310, 1376, 2315, 2589
AlwI	GGATC	7 cut(s)	896, 1995, 2071, 2157, 2193, 2900, 2913
AlwNI	CAGNNNCTG	2 cut(s)	725, 2843
Ama87I	CYCGRG	1 cut(s)	3111
AoxI	GGCC	8 cut(s)	1399, 1448, 1773, 1882, 1945, 2557, 2591, 2828
ApeKI	GCWGC	6 cut(s)	594, 775, 1695, 2009, 2654, 2789
ApoI	RAATTY	8 cut(s)	145, 1094, 1462, 2168, 2367, 2433, 2665, 3134
Asp700I	GAANNNNTTC	2 cut(s)	319, 1269
Asp718I	GGTACC	1 cut(s)	2741
AspLEI	GCGC	2 cut(s)	77, 581
AsuC2I	CCSGG	1 cut(s)	621
AsuII	TTCGAA	1 cut(s)	2795
AsuNHI	GCTAGC	1 cut(s)	1553
AvaI	CYCGRG	1 cut(s)	3111
AvaII	GGWCC	7 cut(s)	481, 812, 1362, 2110, 2469, 2771, 2988
BaeGI	GKGCMC	2 cut(s)	706, 3081
BalI	TGGCCA	3 cut(s)	1775, 1884, 2593
BamHI	GGATCC	1 cut(s)	2905
BanI	GGYRCC	2 cut(s)	703, 2741
BbsI	GAAGAC	1 cut(s)	2360
BbvI	GCAGC	6 cut(s)	606, 787, 1707, 1996, 2641, 2776
BceAI	ACGGC	2 cut(s)	84, 668
BciT130I	CCWGG	5 cut(s)	239, 298, 1488, 2844, 2889
BclI	TGATCA	2 cut(s)	571, 964
BcnI	CCSGG	1 cut(s)	621
BfaI	CTAG	8 cut(s)	339, 629, 807, 867, 912, 1554, 2985, 3156
BfmI	CTRYAG	3 cut(s)	466, 611, 2373
BfuAI	ACCTGC	1 cut(s)	1202
BglII	AGATCT	1 cut(s)	3041
BisI	GCNGC	8 cut(s)	73, 351, 595, 776, 1696, 2010, 2655, 2790
BlsI	GCNGC	8 cut(s)	74, 352, 596, 777, 1697, 2011, 2656, 2791
BmcAI	AGTACT	2 cut(s)	805, 2197
Bme1390I	CCNGG	6 cut(s)	239, 298, 621, 1488, 2844, 2889
Bme18I	GGWCC	7 cut(s)	481, 812, 1362, 2110, 2469, 2771, 2988
BmeT110I	CYCGRG	1 cut(s)	3111
BmrFI	CCNGG	6 cut(s)	239, 298, 621, 1488, 2844, 2889
BmrI	ACTGGG	4 cut(s)	1345, 1612, 1874, 2835
BmtI	GCTAGC	1 cut(s)	1557
BmuI	ACTGGG	4 cut(s)	1345, 1612, 1874, 2835
BoxI	GACNNNNGTC	1 cut(s)	1181
BpiI	GAAGAC	1 cut(s)	2360
BplI	GAGNNNNNCTC	2 cut(s)	3112, 3144
Bpu14I	TTCGAA	1 cut(s)	2795
BpuEI	CTTGAG	4 cut(s)	800, 1519, 1714, 2038
BpuMI	CCSGG	1 cut(s)	621
BsaAI	YACGTR	1 cut(s)	2684
BsaBI	GATNNNNATC	2 cut(s)	570, 963
BsaHI	GRCGYC	2 cut(s)	624, 2405
BsaJI	CCNNGG	5 cut(s)	237, 658, 2842, 2888, 3110
BsaWI	WCCGGW	3 cut(s)	427, 1632, 2466
Bsc4I	CCNNNNNNNGG	8 cut(s)	244, 492, 752, 1705, 1782, 2547, 2953, 3150
Bse1I	ACTGG	8 cut(s)	610, 1241, 1340, 1607, 1828, 1869, 1915, 2830
Bse3DI	GCAATG	2 cut(s)	191, 291
Bse8I	GATNNNNATC	2 cut(s)	570, 963
BseBI	CCWGG	5 cut(s)	239, 298, 1488, 2844, 2889
BseDI	CCNNGG	5 cut(s)	237, 658, 2842, 2888, 3110
BseJI	GATNNNNATC	2 cut(s)	570, 963
BseLI	CCNNNNNNNGG	8 cut(s)	244, 492, 752, 1705, 1782, 2547, 2953, 3150
BseMI	GCAATG	2 cut(s)	191, 291
BseMII	CTCAG	6 cut(s)	532, 728, 2203, 2648, 2705, 2943
BseNI	ACTGG	8 cut(s)	610, 1241, 1340, 1607, 1828, 1869, 1915, 2830
BseRI	GAGGAG	3 cut(s)	24, 27, 208
BseSI	GKGCMC	2 cut(s)	706, 3081
BseXI	GCAGC	6 cut(s)	606, 787, 1707, 1996, 2641, 2776
BsgI	GTGCAG	4 cut(s)	263, 613, 684, 794
Bsh1236I	CGCG	4 cut(s)	75, 1101, 2487, 2808
BshFI	GGCC	8 cut(s)	1401, 1450, 1775, 1884, 1947, 2559, 2593, 2830
BshNI	GGYRCC	2 cut(s)	703, 2741
BsiHKCI	CYCGRG	1 cut(s)	3111
BsiSI	CCGG	4 cut(s)	428, 620, 1633, 2467
BsiWI	CGTACG	1 cut(s)	2809
BslFI	GGGAC	7 cut(s)	467, 673, 1348, 2036, 2101, 2201, 2825
BslI	CCNNNNNNNGG	8 cut(s)	244, 492, 752, 1705, 1782, 2547, 2953, 3150
BsmFI	GGGAC	7 cut(s)	467, 673, 1348, 2036, 2101, 2201, 2825
BsmI	GAATGC	2 cut(s)	269, 1115
BsnI	GGCC	8 cut(s)	1401, 1450, 1775, 1884, 1947, 2559, 2593, 2830
BsoBI	CYCGRG	1 cut(s)	3111
Bsp119I	TTCGAA	1 cut(s)	2795
Bsp1286I	GDGCHC	2 cut(s)	706, 3081
Bsp1407I	TGTACA	1 cut(s)	3007
Bsp68I	TCGCGA	1 cut(s)	2487
BspANI	GGCC	8 cut(s)	1401, 1450, 1775, 1884, 1947, 2559, 2593, 2830
BspCNI	CTCAG	6 cut(s)	531, 727, 2202, 2647, 2706, 2944
BspFNI	CGCG	4 cut(s)	75, 1101, 2487, 2808
BspHI	TCATGA	1 cut(s)	2317
BspMAI	CTGCAG	1 cut(s)	615
BspMI	ACCTGC	1 cut(s)	1202
BspOI	GCTAGC	1 cut(s)	1557
BspPI	GGATC	7 cut(s)	896, 1995, 2071, 2157, 2193, 2900, 2913
BspT104I	TTCGAA	1 cut(s)	2795
BspT107I	GGYRCC	2 cut(s)	703, 2741
BsrDI	GCAATG	2 cut(s)	191, 291
BsrGI	TGTACA	1 cut(s)	3007
BsrI	ACTGG	8 cut(s)	610, 1241, 1340, 1607, 1828, 1869, 1915, 2830
BssECI	CCNNGG	5 cut(s)	237, 658, 2842, 2888, 3110
BssNAI	GTATAC	1 cut(s)	859
BssNI	GRCGYC	2 cut(s)	624, 2405
BssT1I	CCWWGG	1 cut(s)	658
Bst1107I	GTATAC	1 cut(s)	859
Bst2UI	CCWGG	5 cut(s)	239, 298, 1488, 2844, 2889
Bst4CI	ACNGT	5 cut(s)	46, 53, 367, 1084, 3076
Bst6I	CTCTTC	8 cut(s)	226, 512, 1235, 1896, 2373, 2633, 2701, 3032
BstACI	GRCGYC	2 cut(s)	624, 2405
BstAPI	GCANNNNNTGC	1 cut(s)	1685
BstAUI	TGTACA	1 cut(s)	3007
BstBAI	YACGTR	1 cut(s)	2684
BstBI	TTCGAA	1 cut(s)	2795
BstDEI	CTNAG	8 cut(s)	518, 562, 714, 763, 2189, 2634, 2714, 2952
BstENI	CCTNNNNNAGG	1 cut(s)	1703
BstFNI	CGCG	4 cut(s)	75, 1101, 2487, 2808
BstHHI	GCGC	2 cut(s)	77, 581
BstMWI	GCNNNNNNNGC	5 cut(s)	523, 556, 1382, 1685, 3066
BstNI	CCWGG	5 cut(s)	239, 298, 1488, 2844, 2889
BstNSI	RCATGY	1 cut(s)	1539
BstPAI	GACNNNNGTC	1 cut(s)	1181
BstSCI	CCNGG	6 cut(s)	237, 296, 619, 1486, 2842, 2887
BstSFI	CTRYAG	3 cut(s)	466, 611, 2373
BstSLI	GKGCMC	2 cut(s)	706, 3081
BstSNI	TACGTA	1 cut(s)	2684
BstUI	CGCG	4 cut(s)	75, 1101, 2487, 2808
BstV1I	GCAGC	6 cut(s)	606, 787, 1707, 1996, 2641, 2776
BstV2I	GAAGAC	1 cut(s)	2360
BstX2I	RGATCY	3 cut(s)	2185, 2905, 3041
BstXI	CCANNNNNNTGG	3 cut(s)	822, 984, 2825
BstYI	RGATCY	3 cut(s)	2185, 2905, 3041
BstZ17I	GTATAC	1 cut(s)	859
BsuRI	GGCC	8 cut(s)	1401, 1450, 1775, 1884, 1947, 2559, 2593, 2830
BtgZI	GCGATG	3 cut(s)	612, 1785, 2469
BtsI	GCAGTG	2 cut(s)	672, 1548
BtsIMutI	CAGTG	6 cut(s)	672, 1072, 1089, 1509, 1548, 1600
BtuMI	TCGCGA	1 cut(s)	2487
BveI	ACCTGC	1 cut(s)	1202
CaiI	CAGNNNCTG	2 cut(s)	725, 2843
CciI	TCATGA	1 cut(s)	2317
CfoI	GCGC	2 cut(s)	77, 581
CseI	GACGC	2 cut(s)	613, 2394
Csp6I	GTAC	8 cut(s)	804, 1342, 1821, 2196, 2692, 2742, 2810, 3008
CspCI	CAANNNNNGTGG	4 cut(s)	1768, 1798, 1803, 1833
CviAII	CATG	8 cut(s)	199, 419, 1181, 1439, 1536, 1943, 2318, 2651
CviQI	GTAC	8 cut(s)	804, 1342, 1821, 2196, 2692, 2742, 2810, 3008
DdeI	CTNAG	8 cut(s)	518, 562, 714, 763, 2189, 2634, 2714, 2952
EaeI	YGGCCR	3 cut(s)	1773, 1882, 2591
Eam1104I	CTCTTC	8 cut(s)	226, 512, 1235, 1896, 2373, 2633, 2701, 3032
EarI	CTCTTC	8 cut(s)	226, 512, 1235, 1896, 2373, 2633, 2701, 3032
EciI	GGCGGA	2 cut(s)	139, 952
Eco105I	TACGTA	1 cut(s)	2684
Eco130I	CCWWGG	1 cut(s)	658
Eco32I	GATATC	1 cut(s)	1109
Eco47I	GGWCC	7 cut(s)	481, 812, 1362, 2110, 2469, 2771, 2988
Eco57I	CTGAAG	4 cut(s)	1260, 1877, 2295, 2372
Eco88I	CYCGRG	1 cut(s)	3111
EcoNI	CCTNNNNNAGG	1 cut(s)	1703
EcoO109I	RGGNCCY	4 cut(s)	481, 1362, 2771, 2988
EcoRI	GAATTC	2 cut(s)	2168, 2433
EcoRII	CCWGG	5 cut(s)	237, 296, 1486, 2842, 2887
EcoRV	GATATC	1 cut(s)	1109
EcoT14I	CCWWGG	1 cut(s)	658
EcoT22I	ATGCAT	1 cut(s)	1801
ErhI	CCWWGG	1 cut(s)	658
FaeI	CATG	8 cut(s)	202, 422, 1184, 1442, 1539, 1946, 2321, 2654
FalI	AAGNNNNNCTT	6 cut(s)	387, 419, 1674, 1706, 2156, 2188
FaqI	GGGAC	7 cut(s)	467, 673, 1348, 2036, 2101, 2201, 2825
FatI	CATG	8 cut(s)	198, 418, 1180, 1438, 1535, 1942, 2317, 2650
FauI	CCCGC	2 cut(s)	147, 3088
FauNDI	CATATG	3 cut(s)	907, 2080, 2101
FbaI	TGATCA	2 cut(s)	571, 964
FblI	GTMKAC	3 cut(s)	858, 1425, 1915
Fnu4HI	GCNGC	8 cut(s)	73, 351, 595, 776, 1696, 2010, 2655, 2790
Fsp4HI	GCNGC	8 cut(s)	73, 351, 595, 776, 1696, 2010, 2655, 2790
FspBI	CTAG	8 cut(s)	339, 629, 807, 867, 912, 1554, 2985, 3156
GlaI	GCGC	2 cut(s)	76, 580
GluI	GCNGC	8 cut(s)	73, 351, 595, 776, 1696, 2010, 2655, 2790
HaeIII	GGCC	8 cut(s)	1401, 1450, 1775, 1884, 1947, 2559, 2593, 2830
HapII	CCGG	4 cut(s)	428, 620, 1633, 2467
HgaI	GACGC	2 cut(s)	613, 2394
HhaI	GCGC	2 cut(s)	77, 581
Hin1I	GRCGYC	2 cut(s)	624, 2405
Hin1II	CATG	8 cut(s)	202, 422, 1184, 1442, 1539, 1946, 2321, 2654
Hin6I	GCGC	2 cut(s)	75, 579
HinP1I	GCGC	2 cut(s)	75, 579
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	1 cut(s)	250
HpaII	CCGG	4 cut(s)	428, 620, 1633, 2467
Hpy166II	GTNNAC	5 cut(s)	49, 859, 1426, 1543, 1916
Hpy8I	GTNNAC	5 cut(s)	49, 859, 1426, 1543, 1916
Hpy99I	CGWCG	1 cut(s)	105
HpyCH4III	ACNGT	5 cut(s)	46, 53, 367, 1084, 3076
HpyCH4IV	ACGT	4 cut(s)	1032, 1966, 2683, 2812
HpyF10VI	GCNNNNNNNGC	5 cut(s)	523, 556, 1382, 1685, 3066
HpyF3I	CTNAG	8 cut(s)	518, 562, 714, 763, 2189, 2634, 2714, 2952
HpySE526I	ACGT	4 cut(s)	1032, 1966, 2683, 2812
Hsp92I	GRCGYC	2 cut(s)	624, 2405
Hsp92II	CATG	8 cut(s)	202, 422, 1184, 1442, 1539, 1946, 2321, 2654
HspAI	GCGC	2 cut(s)	75, 579
KflI	GGGWCCC	2 cut(s)	481, 1362
KpnI	GGTACC	1 cut(s)	2745
Ksp22I	TGATCA	2 cut(s)	571, 964
LmnI	GCTCC	2 cut(s)	742, 2469
Lsp1109I	GCAGC	6 cut(s)	606, 787, 1707, 1996, 2641, 2776
MaeI	CTAG	8 cut(s)	339, 629, 807, 867, 912, 1554, 2985, 3156
MaeII	ACGT	4 cut(s)	1032, 1966, 2683, 2812
MaeIII	GTNAC	7 cut(s)	615, 1490, 1906, 1934, 2642, 2679, 3161
MfeI	CAATTG	2 cut(s)	2545, 2996
MflI	RGATCY	3 cut(s)	2185, 2905, 3041
MhlI	GDGCHC	2 cut(s)	706, 3081
MlsI	TGGCCA	3 cut(s)	1775, 1884, 2593
MluI	ACGCGT	1 cut(s)	2806
MluNI	TGGCCA	3 cut(s)	1775, 1884, 2593
MlyI	GAGTC	9 cut(s)	209, 1124, 1171, 2223, 2248, 2350, 2356, 2483, 2650
MmeI	TCCRAC	3 cut(s)	65, 2565, 3168
Mox20I	TGGCCA	3 cut(s)	1775, 1884, 2593
Mph1103I	ATGCAT	1 cut(s)	1801
MroXI	GAANNNNTTC	2 cut(s)	319, 1269
MscI	TGGCCA	3 cut(s)	1775, 1884, 2593
MslI	CAYNNNNRTG	4 cut(s)	1437, 1443, 2649, 2855
Msp20I	TGGCCA	3 cut(s)	1775, 1884, 2593
MspA1I	CMGCKG	1 cut(s)	926
MspI	CCGG	4 cut(s)	428, 620, 1633, 2467
MspR9I	CCNGG	6 cut(s)	239, 298, 621, 1488, 2844, 2889
MunI	CAATTG	2 cut(s)	2545, 2996
Mva1269I	GAATGC	2 cut(s)	269, 1115
MvaI	CCWGG	5 cut(s)	239, 298, 1488, 2844, 2889
MvnI	CGCG	4 cut(s)	75, 1101, 2487, 2808
MwoI	GCNNNNNNNGC	5 cut(s)	523, 556, 1382, 1685, 3066
NciI	CCSGG	1 cut(s)	621
NdeI	CATATG	3 cut(s)	907, 2080, 2101
NheI	GCTAGC	1 cut(s)	1553
NlaIII	CATG	8 cut(s)	202, 422, 1184, 1442, 1539, 1946, 2321, 2654
NmeAIII	GCCGAG	2 cut(s)	1112, 1312
NmuCI	GTSAC	2 cut(s)	1906, 2642
NruI	TCGCGA	1 cut(s)	2487
NsiI	ATGCAT	1 cut(s)	1801
NspI	RCATGY	1 cut(s)	1539
NspV	TTCGAA	1 cut(s)	2795
PagI	TCATGA	1 cut(s)	2317
PaqCI	CACCTGC	1 cut(s)	1202
PciI	ACATGT	1 cut(s)	1535
PctI	GAATGC	2 cut(s)	269, 1115
PdmI	GAANNNNTTC	2 cut(s)	319, 1269
PfeI	GAWTC	9 cut(s)	320, 959, 1250, 1258, 1280, 1629, 1979, 2765, 2797
Pfl23II	CGTACG	1 cut(s)	2809
PflMI	CCANNNNNTGG	2 cut(s)	752, 2547
PkrI	GCNGC	8 cut(s)	74, 352, 596, 777, 1697, 2011, 2656, 2791
PleI	GAGTC	9 cut(s)	209, 1124, 1171, 2222, 2248, 2350, 2355, 2483, 2649
PpsI	GAGTC	9 cut(s)	209, 1124, 1171, 2222, 2248, 2350, 2355, 2483, 2649
Ppu21I	YACGTR	1 cut(s)	2684
PpuMI	RGGWCCY	4 cut(s)	481, 1362, 2771, 2988
PscI	ACATGT	1 cut(s)	1535
PshAI	GACNNNNGTC	1 cut(s)	1181
PsiI	TTATAA	1 cut(s)	2535
Psp5II	RGGWCCY	4 cut(s)	481, 1362, 2771, 2988
Psp6I	CCWGG	5 cut(s)	237, 296, 1486, 2842, 2887
PspGI	CCWGG	5 cut(s)	237, 296, 1486, 2842, 2887
PspLI	CGTACG	1 cut(s)	2809
PspPPI	RGGWCCY	4 cut(s)	481, 1362, 2771, 2988
PstI	CTGCAG	1 cut(s)	615
PstNI	CAGNNNCTG	2 cut(s)	725, 2843
PsuI	RGATCY	3 cut(s)	2185, 2905, 3041
RruI	TCGCGA	1 cut(s)	2487
RsaI	GTAC	8 cut(s)	805, 1343, 1822, 2197, 2693, 2743, 2811, 3009
RsaNI	GTAC	8 cut(s)	804, 1342, 1821, 2196, 2692, 2742, 2810, 3008
RseI	CAYNNNNRTG	4 cut(s)	1437, 1443, 2649, 2855
SatI	GCNGC	8 cut(s)	73, 351, 595, 776, 1696, 2010, 2655, 2790
ScaI	AGTACT	2 cut(s)	805, 2197
SchI	GAGTC	9 cut(s)	209, 1124, 1171, 2223, 2248, 2350, 2356, 2483, 2650
ScrFI	CCNGG	6 cut(s)	239, 298, 621, 1488, 2844, 2889
SduI	GDGCHC	2 cut(s)	706, 3081
SfcI	CTRYAG	3 cut(s)	466, 611, 2373
SfuI	TTCGAA	1 cut(s)	2795
SinI	GGWCC	7 cut(s)	481, 812, 1362, 2110, 2469, 2771, 2988
SmiMI	CAYNNNNRTG	4 cut(s)	1437, 1443, 2649, 2855
SmlI	CTYRAG	4 cut(s)	779, 1498, 1729, 2017
SmoI	CTYRAG	4 cut(s)	779, 1498, 1729, 2017
SnaBI	TACGTA	1 cut(s)	2684
SspI	AATATT	1 cut(s)	1813
SspMI	CTAG	8 cut(s)	339, 629, 807, 867, 912, 1554, 2985, 3156
StyD4I	CCNGG	6 cut(s)	237, 296, 619, 1486, 2842, 2887
StyI	CCWWGG	1 cut(s)	658
TaaI	ACNGT	5 cut(s)	46, 53, 367, 1084, 3076
TaiI	ACGT	4 cut(s)	1035, 1969, 2686, 2815
TaqI	TCGA	3 cut(s)	1846, 2795, 3105
TatI	WGTACW	3 cut(s)	803, 2195, 3007
TauI	GCSGC	2 cut(s)	75, 353
TfiI	GAWTC	9 cut(s)	320, 959, 1250, 1258, 1280, 1629, 1979, 2765, 2797
TscAI	CASTG	6 cut(s)	672, 1072, 1089, 1516, 1555, 1607
TseFI	GTSAC	2 cut(s)	1906, 2642
TseI	GCWGC	6 cut(s)	594, 775, 1695, 2009, 2654, 2789
Tsp45I	GTSAC	2 cut(s)	1906, 2642
TspGWI	ACGGA	2 cut(s)	1866, 2699
TspRI	CASTG	6 cut(s)	672, 1072, 1089, 1516, 1555, 1607
Van91I	CCANNNNNTGG	2 cut(s)	752, 2547
VpaK11BI	GGWCC	7 cut(s)	481, 812, 1362, 2110, 2469, 2771, 2988
XagI	CCTNNNNNAGG	1 cut(s)	1703
XapI	RAATTY	8 cut(s)	145, 1094, 1462, 2168, 2367, 2433, 2665, 3134
XceI	RCATGY	1 cut(s)	1539
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1322
XmiI	GTMKAC	3 cut(s)	858, 1425, 1915
XmnI	GAANNNNTTC	2 cut(s)	319, 1269
XspI	CTAG	8 cut(s)	339, 629, 807, 867, 912, 1554, 2985, 3156
ZrmI	AGTACT	2 cut(s)	805, 2197
Zsp2I	ATGCAT	1 cut(s)	1801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.