Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

13371173 .. 13378722

Gene Structure 7550 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g12720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGCCTTTGTTATATTCGTCCCCGTTTCTTCTTTACCGGCCACCATCAACGGCTCCAACGACCAACCACCACTTCCCTCCTCAACGCCCCGCCACGTGTCCTTCATCCTCTCTACGGCGCCGTTTCGCCACCGAGCGCAAACCCCTTCTCCCGCCGCTCCCACTCCGCTCCGCCAGGGTTTTCAGACACCCCAATTTGCAGTGCTCGTCGTCGTCCTCCTTATCAATGTCGGCCGAGGAGGAGCCCACTTCGACCTCGCAGAGTGGTGAACAGCTGCAGAGTGGCTTCTTGTATTCGAGGGCGTTCTGGGTTAGTGAATCAATGATTGCCTGGAATGTCGATGTTGGAAATGGTTCCTGCTACTTATTTGCTAGTAAAACCGCGGCTTTATCTTGTTCGAGTGATGGAATTCTAGGTGAGGATATAAAAGTAAAGCTTGAAGAAGATAAACATGGGCTTCCGGATAATGTGAAAGAAAAATTTCCACATATCAAAGATTACAGAGCTTTTAATGTGCCCCCTGATTTGGATGCCAAACCTCTTCTCAAATGCCAACTGGCAGTTGCTACTTTCGATGCTAATGGAAGGTGCAGTGATGCCACTGGTCTGCAGTTACCTGGTATTTTAGATGAATTGTTCTTGTATAATGGTCCCCTTGGTGCACTTTATTCAGAAGAATCAGTGTCACTGTACCTTTGGGCGCCTACGGCTCGGGAAGTATGTGTTTGCATCTATAAGGAACCGTTAGGTGGGGGGAATCCCCAAGAAGTTGTGCAACTTGAGGAGGTTAATGGCGTTTGGTGTATTAAAGGGCCAAAAAGTTGGGAAGGCTGCTATTACGTGTATGAAGTGTCTGTATATCATCCTAGCACCTTGAAAATTGAAAAGTGCTATGCAAACGATCCATATGCTAGAGGGCTTTCATCAGATGGCAGGCGGACATTGCTGGTTAATCTTGATTCTGATGATATAAAACCTGAAGGATGGGATAAATTGGCTGATGAGAAACCTGATATAATTTCTTTCTCCGACATAAGTATTTATGAGTTACACATCAGGGATTTCAGTGCCAATGACCAGACTGTGCATTCTGAATTTCGTGGTGGATATCTTGCCTTCACTTTGCAGGATTCAGCGGGTGCATTTCATTTGAAGAAATTATCAAATGCTGGTATTACTCATGTTCATCTACTGCCAGCCTACCAATTTTCAGGGGTTGATGATGAGAAGGAGAATTGGAAGAGTGTAGATTTCAAGATACTTGAAAAGTTTGCTCCAGATTCGGATGAGCAACAAGCTCTTATTACAGCTATCCAAAATGATGATGGATATAACTGGGGGTACAACCCTGTTCTCTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTACATACCGTGCAATTGAGTTCAGGAAGATGGTCCAGGCGCTTAACCGTTACGGCCTTCGTGTTGTCTTGGATGCTGTCTACAATCATTTGTCTGAAAGTGGGCCATTTAGTGTGAATTCTGTTCTTGATAAGATTGTTCCAGGATACTACTTAAGAAGAAATACGGATGGCTTTATCGAGAATAGTACATGTGTGAACAACACTGCTAGTGAGCATTTTATGGTTGAGCGTTTAATTGTTGATGATCTATTGCACTGGGCGGTTGATTACAAGGTTGATGGGTTCCGGTTCGATCTTATGGGTCATATAATGAGAAGAACAATGGAGAAAGCAAAAGATGCACTCTGCAGCCTAACAAAGGAAAGGAATGGAGTTGATGGCTCAAGTATCTATATATATGGCGAAGGATGGGATTTTGGTGAAGTTGCCAATAATGGACGTGGAATAAATGCGTCACAGTCCAATCTTCGTGGAACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTTGGTGGATCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACCGGTCTACTCTTGCAGCCTAATGGTCACGATCATGGTCCAGAAAGTGTTGCAAAACACATGCTTGCTGAATCAAAGGATCACATTCAGGTTGGAATGGCTGCAAACCTGAGGGATTTTGTGCTAACCGATTGTGAAGGGAAAGAGGTAAAAGGAGCAGAAGTTTTGACCTATGGCGGGACACCTGTAGCATATACTTTATCTCCTACTGAAACTATCAATTATGTTTCTGCTCATGACAACGAAACTCTGTTCGACATTGTGGCTCTGAAGACTTCGATGGAAATTTCTTTAGAGGAGAGATGCAGGATAAATCACTTGGCGACGAGTATAATAGCACTCGGACAGGGGATACCATTTTTCCACGCCGGCGATGAGCTGCTTCGGTCAAAATCACTTGATCGATCGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

791

Amino Acids

87.62

Weight (kDa)

5.38

Isoelectric Point (pI)

40.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	99 - 211	4.2e-33	Pullulanase N2 domain
CBM_48	PF02922	218 - 304	3.1e-08	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	117, 700
AccBSI	CCGCTC	2 cut(s)	157, 168
AccI	GTMKAC	2 cut(s)	1476, 1966
AccII	CGCG	1 cut(s)	383
AccIII	TCCGGA	1 cut(s)	460
AclWI	GGATC	3 cut(s)	896, 1930, 2046
AcoI	YGGCCR	3 cut(s)	38, 231, 1933
AcsI	RAATTY	5 cut(s)	408, 479, 1094, 1513, 2244
AcuI	CTGAAG	3 cut(s)	999, 1928, 2249
AcvI	CACGTG	1 cut(s)	96
AcyI	GRCGYC	2 cut(s)	118, 701
AfaI	GTAC	4 cut(s)	692, 1343, 1399, 1585
AfiI	CCNNNNNNNGG	5 cut(s)	114, 526, 749, 1756, 2136
AflII	CTTAAG	1 cut(s)	1549
AflIII	ACRYGT	3 cut(s)	95, 840, 1586
AgeI	ACCGGT	1 cut(s)	1961
AgsI	TTSAA	6 cut(s)	440, 877, 884, 1153, 1255, 1265
AjiI	CACGTC	1 cut(s)	1838
AjnI	CCWGG	5 cut(s)	173, 329, 616, 1431, 1537
AjuI	GAANNNNNNNTTGG	2 cut(s)	56, 88
AluBI	AGCT	7 cut(s)	274, 436, 506, 1298, 1310, 1377, 2338
AluI	AGCT	7 cut(s)	274, 436, 506, 1298, 1310, 1377, 2338
Alw21I	GWGCWC	2 cut(s)	206, 664
Alw44I	GTGCAC	1 cut(s)	660
AlwI	GGATC	3 cut(s)	896, 1930, 2046
Ama87I	CYCGRG	1 cut(s)	711
Aor13HI	TCCGGA	1 cut(s)	460
AoxI	GGCC	6 cut(s)	38, 231, 812, 1450, 1499, 1933
ApaLI	GTGCAC	1 cut(s)	660
ApeKI	GCWGC	6 cut(s)	274, 831, 1746, 1975, 2060, 2338
ApoI	RAATTY	5 cut(s)	408, 479, 1094, 1513, 2244
AsiGI	ACCGGT	1 cut(s)	1961
Asp700I	GAANNNNTTC	2 cut(s)	352, 480
AspLEI	GCGC	4 cut(s)	120, 138, 703, 1438
AspS9I	GGNCC	6 cut(s)	650, 812, 1363, 1429, 1499, 1997
AsuHPI	GGTGA	3 cut(s)	278, 428, 1829
AvaI	CYCGRG	1 cut(s)	711
AvaII	GGWCC	4 cut(s)	650, 1363, 1429, 1997
AxyI	CCTNAGG	1 cut(s)	2069
BaeGI	GKGCMC	2 cut(s)	519, 664
BalI	TGGCCA	1 cut(s)	1935
BanI	GGYRCC	2 cut(s)	117, 700
BanII	GRGCYC	1 cut(s)	246
BbrPI	CACGTG	1 cut(s)	96
BbsI	GAAGAC	1 cut(s)	2237
Bbv12I	GWGCWC	2 cut(s)	206, 664
BbvI	GCAGC	6 cut(s)	261, 818, 1758, 1987, 2047, 2325
BceAI	ACGGC	5 cut(s)	66, 105, 131, 723, 1465
BcgI	CGANNNNNNTGC	2 cut(s)	890, 924
BciT130I	CCWGG	5 cut(s)	175, 331, 618, 1433, 1539
BciVI	GTATCC	2 cut(s)	1535, 2304
BfaI	CTAG	5 cut(s)	372, 413, 867, 912, 1605
BfmI	CTRYAG	4 cut(s)	275, 608, 1744, 2145
BfoI	RGCGCY	3 cut(s)	121, 704, 1439
BfrI	CTTAAG	1 cut(s)	1549
BfuI	GTATCC	2 cut(s)	1535, 2304
BisI	GCNGC	8 cut(s)	155, 275, 384, 832, 1747, 1976, 2061, 2339
BlsI	GCNGC	8 cut(s)	156, 276, 385, 833, 1748, 1977, 2062, 2340
Bme1390I	CCNGG	5 cut(s)	175, 331, 618, 1433, 1539
Bme18I	GGWCC	4 cut(s)	650, 1363, 1429, 1997
BmeT110I	CYCGRG	1 cut(s)	711
BmgBI	CACGTC	1 cut(s)	1838
BmgT120I	GGNCC	6 cut(s)	650, 812, 1363, 1429, 1499, 1997
BmrFI	CCNGG	5 cut(s)	175, 331, 618, 1433, 1539
BmrI	ACTGGG	2 cut(s)	1345, 1663
BmsI	GCATC	8 cut(s)	520, 565, 586, 738, 1459, 1726, 1897, 2252
BmuI	ACTGGG	2 cut(s)	1345, 1663
BpiI	GAAGAC	1 cut(s)	2237
BpmI	CTGGAG	1 cut(s)	1260
BpuEI	CTTGAG	2 cut(s)	800, 1765
Bsa29I	ATCGAT	1 cut(s)	2362
BsaAI	YACGTR	2 cut(s)	96, 841
BsaHI	GRCGYC	2 cut(s)	118, 701
BsaJI	CCNNGG	4 cut(s)	174, 234, 381, 655
BsaWI	WCCGGW	3 cut(s)	460, 1683, 1961
BsaXI	ACNNNNNCTCC	6 cut(s)	132, 162, 2106, 2136, 2146, 2176
Bsc4I	CCNNNNNNNGG	5 cut(s)	114, 526, 749, 1756, 2136
Bse118I	RCCGGY	3 cut(s)	36, 1961, 2327
Bse1I	ACTGG	5 cut(s)	561, 607, 1340, 1658, 1879
Bse21I	CCTNAGG	1 cut(s)	2069
Bse3DI	GCAATG	1 cut(s)	941
BseAI	TCCGGA	1 cut(s)	460
BseBI	CCWGG	5 cut(s)	175, 331, 618, 1433, 1539
BseCI	ATCGAT	1 cut(s)	2362
BseDI	CCNNGG	4 cut(s)	174, 234, 381, 655
BseLI	CCNNNNNNNGG	5 cut(s)	114, 526, 749, 1756, 2136
BseMI	GCAATG	1 cut(s)	941
BseMII	CTCAG	1 cut(s)	2060
BseNI	ACTGG	5 cut(s)	561, 607, 1340, 1658, 1879
BseRI	GAGGAG	5 cut(s)	69, 251, 254, 797, 2270
BseSI	GKGCMC	2 cut(s)	519, 664
BseX3I	CGGCCG	1 cut(s)	231
BseXI	GCAGC	6 cut(s)	261, 818, 1758, 1987, 2047, 2325
BsgI	GTGCAG	1 cut(s)	610
Bsh1236I	CGCG	1 cut(s)	383
Bsh1285I	CGRYCG	2 cut(s)	234, 2366
BshFI	GGCC	6 cut(s)	40, 233, 814, 1452, 1501, 1935
BshNI	GGYRCC	2 cut(s)	117, 700
BshTI	ACCGGT	1 cut(s)	1961
BshVI	ATCGAT	1 cut(s)	2362
BsiEI	CGRYCG	2 cut(s)	234, 2366
BsiHKAI	GWGCWC	2 cut(s)	206, 664
BsiHKCI	CYCGRG	1 cut(s)	711
BsiSI	CCGG	5 cut(s)	37, 461, 1684, 1962, 2328
BslFI	GGGAC	4 cut(s)	4, 636, 1349, 2152
BslI	CCNNNNNNNGG	5 cut(s)	114, 526, 749, 1756, 2136
BsmFI	GGGAC	4 cut(s)	4, 636, 1349, 2152
BsmI	GAATGC	1 cut(s)	1087
BsnI	GGCC	6 cut(s)	40, 233, 814, 1452, 1501, 1935
BsoBI	CYCGRG	1 cut(s)	711
Bsp1286I	GDGCHC	4 cut(s)	206, 246, 519, 664
Bsp13I	TCCGGA	1 cut(s)	460
Bsp143I	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
BspANI	GGCC	6 cut(s)	40, 233, 814, 1452, 1501, 1935
BspCNI	CTCAG	1 cut(s)	2061
BspDI	ATCGAT	1 cut(s)	2362
BspEI	TCCGGA	1 cut(s)	460
BspFNI	CGCG	1 cut(s)	383
BspHI	TCATGA	1 cut(s)	2194
BspMAI	CTGCAG	3 cut(s)	279, 612, 1748
BspPI	GGATC	3 cut(s)	896, 1930, 2046
BspT107I	GGYRCC	2 cut(s)	117, 700
BspTI	CTTAAG	1 cut(s)	1549
BsrBI	CCGCTC	2 cut(s)	157, 168
BsrDI	GCAATG	1 cut(s)	941
BsrFI	RCCGGY	3 cut(s)	36, 1961, 2327
BsrI	ACTGG	5 cut(s)	561, 607, 1340, 1658, 1879
BssAI	RCCGGY	3 cut(s)	36, 1961, 2327
BssECI	CCNNGG	4 cut(s)	174, 234, 381, 655
BssMI	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
BssNI	GRCGYC	2 cut(s)	118, 701
BssT1I	CCWWGG	1 cut(s)	655
Bst2UI	CCWGG	5 cut(s)	175, 331, 618, 1433, 1539
Bst4CI	ACNGT	6 cut(s)	690, 744, 1084, 1406, 1445, 1857
Bst6I	CTCTTC	3 cut(s)	546, 1235, 1947
BstACI	GRCGYC	2 cut(s)	118, 701
BstAFI	CTTAAG	1 cut(s)	1549
BstAPI	GCANNNNNTGC	2 cut(s)	1388, 1736
BstBAI	YACGTR	2 cut(s)	96, 841
BstC8I	GCNNGC	4 cut(s)	935, 1197, 2025, 2329
BstDEI	CTNAG	1 cut(s)	2069
BstDSI	CCRYGG	1 cut(s)	381
BstENI	CCTNNNNNAGG	1 cut(s)	1754
BstFNI	CGCG	1 cut(s)	383
BstH2I	RGCGCY	3 cut(s)	121, 704, 1439
BstHHI	GCGC	4 cut(s)	120, 138, 703, 1438
BstKTI	GATC	9 cut(s)	904, 1645, 1693, 1897, 1925, 1993, 2041, 2362, 2366
BstMBI	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
BstMCI	CGRYCG	2 cut(s)	234, 2366
BstMWI	GCNNNNNNNGC	4 cut(s)	707, 943, 1388, 1736
BstNI	CCWGG	5 cut(s)	175, 331, 618, 1433, 1539
BstNSI	RCATGY	2 cut(s)	1590, 2023
BstSCI	CCNGG	5 cut(s)	173, 329, 616, 1431, 1537
BstSFI	CTRYAG	4 cut(s)	275, 608, 1744, 2145
BstSLI	GKGCMC	2 cut(s)	519, 664
BstUI	CGCG	1 cut(s)	383
BstV1I	GCAGC	6 cut(s)	261, 818, 1758, 1987, 2047, 2325
BstV2I	GAAGAC	1 cut(s)	2237
BstX2I	RGATCY	1 cut(s)	1922
BstXI	CCANNNNNNTGG	1 cut(s)	822
BstYI	RGATCY	1 cut(s)	1922
BstZI	CGGCCG	1 cut(s)	231
Bsu15I	ATCGAT	1 cut(s)	2362
Bsu36I	CCTNAGG	1 cut(s)	2069
BsuI	GTATCC	2 cut(s)	1535, 2304
BsuRI	GGCC	6 cut(s)	40, 233, 814, 1452, 1501, 1935
BsuTUI	ATCGAT	1 cut(s)	2362
BtgI	CCRYGG	1 cut(s)	381
BtgZI	GCGATG	1 cut(s)	2346
BtrI	CACGTC	1 cut(s)	1838
BtsI	GCAGTG	3 cut(s)	206, 598, 1599
BtsIMutI	CAGTG	8 cut(s)	206, 598, 600, 686, 687, 1072, 1599, 1651
Cac8I	GCNNGC	4 cut(s)	935, 1197, 2025, 2329
CciI	TCATGA	1 cut(s)	2194
CfoI	GCGC	4 cut(s)	120, 138, 703, 1438
Cfr10I	RCCGGY	3 cut(s)	36, 1961, 2327
Cfr13I	GGNCC	6 cut(s)	650, 812, 1363, 1429, 1499, 1997
Cfr42I	CCGCGG	1 cut(s)	384
ClaI	ATCGAT	1 cut(s)	2362
CseI	GACGC	1 cut(s)	1839
CsiI	ACCWGGT	1 cut(s)	616
Csp6I	GTAC	4 cut(s)	691, 1342, 1398, 1584
CspAI	ACCGGT	1 cut(s)	1961
CspCI	CAANNNNNGTGG	2 cut(s)	1849, 1884
CviAII	CATG	6 cut(s)	452, 1181, 1587, 1994, 2020, 2195
CviQI	GTAC	4 cut(s)	691, 1342, 1398, 1584
DdeI	CTNAG	1 cut(s)	2069
DinI	GGCGCC	2 cut(s)	119, 702
DpnI	GATC	9 cut(s)	903, 1644, 1692, 1896, 1924, 1992, 2040, 2361, 2365
DpnII	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
EaeI	YGGCCR	3 cut(s)	38, 231, 1933
EagI	CGGCCG	1 cut(s)	231
Eam1104I	CTCTTC	3 cut(s)	546, 1235, 1947
EarI	CTCTTC	3 cut(s)	546, 1235, 1947
EciI	GGCGGA	2 cut(s)	160, 952
EclXI	CGGCCG	1 cut(s)	231
Eco130I	CCWWGG	1 cut(s)	655
Eco24I	GRGCYC	1 cut(s)	246
Eco32I	GATATC	1 cut(s)	1109
Eco47I	GGWCC	4 cut(s)	650, 1363, 1429, 1997
Eco52I	CGGCCG	1 cut(s)	231
Eco57I	CTGAAG	3 cut(s)	999, 1928, 2249
Eco72I	CACGTG	1 cut(s)	96
Eco81I	CCTNAGG	1 cut(s)	2069
Eco88I	CYCGRG	1 cut(s)	711
EcoNI	CCTNNNNNAGG	1 cut(s)	1754
EcoO109I	RGGNCCY	1 cut(s)	1363
EcoRI	GAATTC	2 cut(s)	408, 1513
EcoRII	CCWGG	5 cut(s)	173, 329, 616, 1431, 1537
EcoRV	GATATC	1 cut(s)	1109
EcoT14I	CCWWGG	1 cut(s)	655
EcoT38I	GRGCYC	1 cut(s)	246
EgeI	GGCGCC	2 cut(s)	119, 702
EheI	GGCGCC	2 cut(s)	119, 702
ErhI	CCWWGG	1 cut(s)	655
FaeI	CATG	6 cut(s)	455, 1184, 1590, 1997, 2023, 2198
FalI	AAGNNNNNCTT	2 cut(s)	420, 452
FaqI	GGGAC	4 cut(s)	4, 636, 1349, 2152
FatI	CATG	6 cut(s)	451, 1180, 1586, 1993, 2019, 2194
FauI	CCCGC	4 cut(s)	97, 159, 1129, 2129
FauNDI	CATATG	1 cut(s)	907
FblI	GTMKAC	2 cut(s)	1476, 1966
Fnu4HI	GCNGC	8 cut(s)	155, 275, 384, 832, 1747, 1976, 2061, 2339
FriOI	GRGCYC	1 cut(s)	246
Fsp4HI	GCNGC	8 cut(s)	155, 275, 384, 832, 1747, 1976, 2061, 2339
FspBI	CTAG	5 cut(s)	372, 413, 867, 912, 1605
GlaI	GCGC	4 cut(s)	119, 137, 702, 1437
GluI	GCNGC	8 cut(s)	155, 275, 384, 832, 1747, 1976, 2061, 2339
GsuI	CTGGAG	1 cut(s)	1260
HaeII	RGCGCY	3 cut(s)	121, 704, 1439
HaeIII	GGCC	6 cut(s)	40, 233, 814, 1452, 1501, 1935
HapII	CCGG	5 cut(s)	37, 461, 1684, 1962, 2328
HgaI	GACGC	1 cut(s)	1839
HhaI	GCGC	4 cut(s)	120, 138, 703, 1438
Hin1I	GRCGYC	2 cut(s)	118, 701
Hin1II	CATG	6 cut(s)	455, 1184, 1590, 1997, 2023, 2198
Hin6I	GCGC	4 cut(s)	118, 136, 701, 1436
HinP1I	GCGC	4 cut(s)	118, 136, 701, 1436
HindIII	AAGCTT	1 cut(s)	434
HinfI	GANTC	7 cut(s)	317, 677, 757, 959, 1130, 1280, 2030
HpaII	CCGG	5 cut(s)	37, 461, 1684, 1962, 2328
HphI	GGTGA	3 cut(s)	278, 428, 1829
Hpy166II	GTNNAC	5 cut(s)	269, 662, 1477, 1594, 1967
Hpy8I	GTNNAC	5 cut(s)	269, 662, 1477, 1594, 1967
Hpy99I	CGWCG	3 cut(s)	211, 214, 2287
HpyCH4III	ACNGT	6 cut(s)	690, 744, 1084, 1406, 1445, 1857
HpyCH4IV	ACGT	3 cut(s)	95, 840, 1837
HpyF10VI	GCNNNNNNNGC	4 cut(s)	707, 943, 1388, 1736
HpyF3I	CTNAG	1 cut(s)	2069
HpySE526I	ACGT	3 cut(s)	95, 840, 1837
Hsp92I	GRCGYC	2 cut(s)	118, 701
Hsp92II	CATG	6 cut(s)	455, 1184, 1590, 1997, 2023, 2198
HspAI	GCGC	4 cut(s)	118, 136, 701, 1436
KasI	GGCGCC	2 cut(s)	117, 700
KflI	GGGWCCC	1 cut(s)	1363
Kpn2I	TCCGGA	1 cut(s)	460
KroI	GCCGGC	1 cut(s)	2327
KroNI	GCCGGC	1 cut(s)	2329
KspI	CCGCGG	1 cut(s)	384
Kzo9I	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
LmnI	GCTCC	6 cut(s)	58, 162, 173, 241, 1279, 2114
Lsp1109I	GCAGC	6 cut(s)	261, 818, 1758, 1987, 2047, 2325
LweI	GCATC	8 cut(s)	520, 565, 586, 738, 1459, 1726, 1897, 2252
MabI	ACCWGGT	1 cut(s)	616
MaeI	CTAG	5 cut(s)	372, 413, 867, 912, 1605
MaeII	ACGT	3 cut(s)	95, 840, 1837
MaeIII	GTNAC	7 cut(s)	612, 684, 1047, 1445, 1851, 1957, 1985
MalI	GATC	9 cut(s)	903, 1644, 1692, 1896, 1924, 1992, 2040, 2361, 2365
MbiI	CCGCTC	2 cut(s)	157, 168
MboI	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
MfeI	CAATTG	1 cut(s)	1410
MflI	RGATCY	1 cut(s)	1922
MhlI	GDGCHC	4 cut(s)	206, 246, 519, 664
MlsI	TGGCCA	1 cut(s)	1935
MluNI	TGGCCA	1 cut(s)	1935
Mly113I	GGCGCC	2 cut(s)	118, 701
MmeI	TCCRAC	4 cut(s)	80, 325, 1053, 2032
Mox20I	TGGCCA	1 cut(s)	1935
MreI	CGCCGGCG	1 cut(s)	2327
MroI	TCCGGA	1 cut(s)	460
MroNI	GCCGGC	1 cut(s)	2327
MroXI	GAANNNNTTC	2 cut(s)	352, 480
MscI	TGGCCA	1 cut(s)	1935
MseI	TTAA	8 cut(s)	510, 789, 807, 951, 1440, 1550, 1631, 1890
MslI	CAYNNNNRTG	1 cut(s)	1992
Msp20I	TGGCCA	1 cut(s)	1935
MspA1I	CMGCKG	3 cut(s)	274, 383, 1136
MspCI	CTTAAG	1 cut(s)	1549
MspI	CCGG	5 cut(s)	37, 461, 1684, 1962, 2328
MspR9I	CCNGG	5 cut(s)	175, 331, 618, 1433, 1539
MunI	CAATTG	1 cut(s)	1410
Mva1269I	GAATGC	1 cut(s)	1087
MvaI	CCWGG	5 cut(s)	175, 331, 618, 1433, 1539
MvnI	CGCG	1 cut(s)	383
MwoI	GCNNNNNNNGC	4 cut(s)	707, 943, 1388, 1736
NaeI	GCCGGC	1 cut(s)	2329
NarI	GGCGCC	2 cut(s)	118, 701
NdeI	CATATG	1 cut(s)	907
NdeII	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
NgoMIV	GCCGGC	1 cut(s)	2327
NlaIII	CATG	6 cut(s)	455, 1184, 1590, 1997, 2023, 2198
NmeAIII	GCCGAG	1 cut(s)	259
NmuCI	GTSAC	4 cut(s)	684, 1851, 1957, 1985
NspI	RCATGY	2 cut(s)	1590, 2023
PagI	TCATGA	1 cut(s)	2194
PciI	ACATGT	1 cut(s)	1586
PcsI	WCGNNNNNNNCGW	1 cut(s)	56
PctI	GAATGC	1 cut(s)	1087
PdiI	GCCGGC	1 cut(s)	2329
PdmI	GAANNNNTTC	2 cut(s)	352, 480
PfeI	GAWTC	7 cut(s)	317, 677, 757, 959, 1130, 1280, 2030
PfoI	TCCNGGA	1 cut(s)	1537
PinAI	ACCGGT	1 cut(s)	1961
PkrI	GCNGC	8 cut(s)	156, 276, 385, 833, 1748, 1977, 2062, 2340
Ple19I	CGATCG	1 cut(s)	2366
PluTI	GGCGCC	2 cut(s)	121, 704
PmaCI	CACGTG	1 cut(s)	96
PmlI	CACGTG	1 cut(s)	96
Ppu21I	YACGTR	2 cut(s)	96, 841
PpuMI	RGGWCCY	1 cut(s)	1363
PscI	ACATGT	1 cut(s)	1586
Psp5II	RGGWCCY	1 cut(s)	1363
Psp6I	CCWGG	5 cut(s)	173, 329, 616, 1431, 1537
PspCI	CACGTG	1 cut(s)	96
PspGI	CCWGG	5 cut(s)	173, 329, 616, 1431, 1537
PspPI	GGNCC	6 cut(s)	650, 812, 1363, 1429, 1499, 1997
PspPPI	RGGWCCY	1 cut(s)	1363
PstI	CTGCAG	3 cut(s)	279, 612, 1748
PsuI	RGATCY	1 cut(s)	1922
PvuI	CGATCG	1 cut(s)	2366
PvuII	CAGCTG	1 cut(s)	274
RsaI	GTAC	4 cut(s)	692, 1343, 1399, 1585
RsaNI	GTAC	4 cut(s)	691, 1342, 1398, 1584
RseI	CAYNNNNRTG	1 cut(s)	1992
SacII	CCGCGG	1 cut(s)	384
SaqAI	TTAA	8 cut(s)	510, 789, 807, 951, 1440, 1550, 1631, 1890
SatI	GCNGC	8 cut(s)	155, 275, 384, 832, 1747, 1976, 2061, 2339
Sau3AI	GATC	9 cut(s)	901, 1642, 1690, 1894, 1922, 1990, 2038, 2359, 2363
Sau96I	GGNCC	6 cut(s)	650, 812, 1363, 1429, 1499, 1997
ScrFI	CCNGG	5 cut(s)	175, 331, 618, 1433, 1539
SduI	GDGCHC	4 cut(s)	206, 246, 519, 664
SexAI	ACCWGGT	1 cut(s)	616
SfaNI	GCATC	8 cut(s)	520, 565, 586, 738, 1459, 1726, 1897, 2252
SfcI	CTRYAG	4 cut(s)	275, 608, 1744, 2145
SfoI	GGCGCC	2 cut(s)	119, 702
Sfr303I	CCGCGG	1 cut(s)	384
SgrAI	CRCCGGYG	1 cut(s)	2327
SgrBI	CCGCGG	1 cut(s)	384
SinI	GGWCC	4 cut(s)	650, 1363, 1429, 1997
SmiMI	CAYNNNNRTG	1 cut(s)	1992
SmlI	CTYRAG	3 cut(s)	779, 1549, 1780
SmoI	CTYRAG	3 cut(s)	779, 1549, 1780
SspDI	GGCGCC	2 cut(s)	117, 700
SspMI	CTAG	5 cut(s)	372, 413, 867, 912, 1605
StyD4I	CCNGG	5 cut(s)	173, 329, 616, 1431, 1537
StyI	CCWWGG	1 cut(s)	655
TaaI	ACNGT	6 cut(s)	690, 744, 1084, 1406, 1445, 1857
TaiI	ACGT	3 cut(s)	98, 843, 1840
TaqII	GACCGA	1 cut(s)	2334
TatI	WGTACW	1 cut(s)	1583
TauI	GCSGC	2 cut(s)	157, 386
TfiI	GAWTC	7 cut(s)	317, 677, 757, 959, 1130, 1280, 2030
Tru1I	TTAA	8 cut(s)	510, 789, 807, 951, 1440, 1550, 1631, 1890
Tru9I	TTAA	8 cut(s)	510, 789, 807, 951, 1440, 1550, 1631, 1890
TscAI	CASTG	8 cut(s)	206, 598, 607, 687, 693, 1072, 1606, 1658
TseFI	GTSAC	4 cut(s)	684, 1851, 1957, 1985
TseI	GCWGC	6 cut(s)	274, 831, 1746, 1975, 2060, 2338
Tsp45I	GTSAC	4 cut(s)	684, 1851, 1957, 1985
TspDTI	ATGAA	6 cut(s)	93, 645, 861, 912, 1136, 1175
TspGWI	ACGGA	1 cut(s)	1577
TspRI	CASTG	8 cut(s)	206, 598, 607, 687, 693, 1072, 1606, 1658
Vha464I	CTTAAG	1 cut(s)	1549
VneI	GTGCAC	1 cut(s)	660
VpaK11BI	GGWCC	4 cut(s)	650, 1363, 1429, 1997
XagI	CCTNNNNNAGG	1 cut(s)	1754
XapI	RAATTY	5 cut(s)	408, 479, 1094, 1513, 2244
XceI	RCATGY	2 cut(s)	1590, 2023
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1322
XmiI	GTMKAC	2 cut(s)	1476, 1966
XmnI	GAANNNNTTC	2 cut(s)	352, 480
XspI	CTAG	5 cut(s)	372, 413, 867, 912, 1605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.