Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

52316692 .. 52327476

Gene Structure 10785 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG297600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2895 bp

ATGCCTTTATGTACTTGTTCTCCACTTCTTCCGCCTCCAACGGTCAACAGTCATTCCCTTCCTCACCGCCGCGCCTATTCTTCATCCTCCATACGCCGTCGTTTCACCTCCCAAGCCAAACCCATTTTCTCCTTCCCGCCAAATTCCGCCAAGTTTTCCAGAACACCGCTACTTCAATGCTCGTCCTCATTGCCCATGACTGCCGAACAAGACTCCTCTTCAACCTCACAACCCCAGGTGCAGGGAAGCTTGTTGAAATCAAGGGCATTCTGGGTTAGTGAATCAATCATTGCCTGGAATGTGGATGCTGGAAATGATTCGTGCTACTTATTCGCTAGTGGAACTGCGGCGTTGTCATTAACGGGTGATGGAGTTTTGGGTGAGGATGTAAAAGTTCAGCTTCAAGAGGATAGACATGGGCTACCGGAAAATGTGAAAACAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAGGGCCCCCCCTGATCTGGATGTCAAACCTCTTCTCAAAGCTCAGTTGGCAGTTGCTACTTTCAGTTTTGATGGAAGGTGCAGTGATGCTACTGGTCTGCAGTTACCGGGTGTCCTAGATGAATTGTTCTCGTATGATGGTCCCCTTGGTGCAGTGTGTTCAAAAGAAGCTGTGTCCCTTTACCTTTGGGCACCTACTTCTCAGGCAGTATCTGTATTGATCTATAAGGAGCCATCGGGTGGAAATCCCTTAGAAATTGTGCAGCTTGAGGAAGTTAATGGTGTTTGGAGTACTAGAGGACCAAAAACTTGGGAAGGATGCTACTATGTGTATGAAGTGTCTGTATACCACCCTAGCACCTTACAAATTGAAAAGTGCTATGCAAATGATCCATATGCTAGAGGGCTTTCAGCAGATGGTAGGCGGACATTGTTCATCAATCTTGATTCTGATCATATAAAACCAGAAGGATGGGATAAATTGGTAGATGAGAAACCTGATATACATTCTTCTTCGGACATAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGCGGTGGATATCTGGCATTCACTTCGCAGGACTCGGCTGGCGTATCTCATTTGAAGAAATTATCAAATGCTGGTATCACTCATGTCCATCTACTGCCAGCCTACCAATTTGCAGGTGTTGATGATGAGAAGGGGACCTGGACGAGTGTAGATTCCAAGATTCTTGAAAAGTTTCCTTCAGATTCATCCGAGCAACAAGCTCTAATTACAGCTATCCAAAATGATGATGGGTATAACTGGGGGTACAACCCTGTCCTTTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTTCATACCGGGCAATTGAGTTTAGGAAGATGGTTCAGGCACTTAATCGTTTTGGCCTTCGTGTTGTCTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGAAAATTCAGTTCTTGATAAGATTGTTCCAGGTTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAATAGCACATGTGTAAACAACACTGCTAGCGAGCATTTTATGGTAGAGCGTTTAATTCTTGATGATCTTTTGCACTGGGCAGTTGATTTTAAGGTTGATGGATTCCGGTTTGACCTTATGGGTCATATAATGAAAAGAACAATGGTGAAAGCAAAAGATGCACTTTGCAGCCTAACAAAGGACAGGGATGGAGTTGATGGCTCAAGTATCTACATATATGGTGAAGGATGGGATTTTGGTGAAGTGGCCAACAATGGGCGTGGAGTAAATGCATCGCAGTTCAATATTTGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTGGGTGGTTCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCAGAAGTTGTTGCAGAACGTATGCTTGCTGAATCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTGAGGGACTTTGTGCTAACCAATTATGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGTGGGACAAGTGTAGCATATGCTTTGGACCCTACTGAAACTGTTAATTATGCTTCAGCTCATGACAATGAAACTCTTTTTGACATCATGAGTTTGAAGACTCCATTTGAAATTTCTGTAGAAGAAAGATGCAGGATAAATCTCTTGGCGTCAAGCATAATAGCACTATCACAGGGAATTCCATTTTTCCACTCCGGCGATGAGATGCTCCGGTCCAAATCACTTGATCGTGACTCATACAATTCTGGTGATTGGTTCAACAGGATTGACTTCACTTATAATTCCAACAATTGGGGTGTTGGCCTTCCTCCAAAAGAGAAAAATGAAAAGAGCTGGCCACTAATGAAACCAAGATTAGCAGAGCCATCCTTTAAGCCTCAGAAGAGTCACATCCATGCTGCTGTGGAGAATTTTTCAGATTTGTTACGTATTAGGTACTCTTCACCACTTTTCCGTCTGAGGACAGCAAATGCTATTCAGGAACGCGTACGTTTCCACAATACTGGGCCTGCATCAGTCCCTGGCGTCATAGTAATGAGCATTGAAGATGGTCATGAAGGACTACCAGGGTTATCTCAACTGGATCCAATCTACTCCTACATTGTGGTTATCGTCAATGCACGCCCAACTGAGGTGTCATATACAAGTTCTTCTATTCGTGCTAGGACCCTTCAATTGCATCCTGTACAGGTTAAGTCCAATGATGAAATAGTGAAGAGATCTACTTATGACATAGGCTCGGGATGCTTTACTGTGCCCGCAAGGACGACATCTGTGTTTGTCGAGCCTCGGGGGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

964

Amino Acids

106.65

Weight (kDa)

5.75

Isoelectric Point (pI)

40.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	86 - 199	2.7e-32	Pullulanase N2 domain
CBM_48	PF02922	206 - 291	2.1e-06	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	791 - 960	5.4e-66	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2373
AarI	CACCTGC	1 cut(s)	1163
Acc36I	ACCTGC	1 cut(s)	1163
AccB1I	GGYRCC	1 cut(s)	664
AccB7I	CCANNNNNTGG	2 cut(s)	713, 2385
AccI	GTMKAC	3 cut(s)	819, 1437, 1927
AccII	CGCG	3 cut(s)	72, 1062, 2580
AclWI	GGATC	5 cut(s)	857, 2007, 2083, 2672, 2685
AcoI	YGGCCR	3 cut(s)	1785, 1894, 2429
AcsI	RAATTY	6 cut(s)	142, 1055, 1474, 2205, 2271, 2503
AcuI	CTGAAG	3 cut(s)	1221, 1889, 2133
AcyI	GRCGYC	2 cut(s)	2243, 2619
AfaI	GTAC	7 cut(s)	13, 766, 1304, 1834, 2531, 2583, 2781
AfiI	CCNNNNNNNGG	8 cut(s)	241, 490, 713, 1321, 1717, 1794, 2385, 2725
AflIII	ACRYGT	2 cut(s)	1547, 2578
AjnI	CCWGG	6 cut(s)	234, 293, 1196, 1498, 2614, 2659
AjuI	GAANNNNNNNTTGG	2 cut(s)	110, 142
AlwI	GGATC	5 cut(s)	857, 2007, 2083, 2672, 2685
AlwNI	CAGNNNCTG	2 cut(s)	686, 2615
Ama87I	CYCGRG	2 cut(s)	2833, 2883
AoxI	GGCC	9 cut(s)	477, 1411, 1460, 1785, 1894, 1957, 2395, 2429, 2600
ApaI	GGGCCC	1 cut(s)	481
ApeKI	GCWGC	4 cut(s)	736, 1707, 2021, 2492
ApoI	RAATTY	6 cut(s)	142, 1055, 1474, 2205, 2271, 2503
Asp700I	GAANNNNTTC	2 cut(s)	316, 1230
AspLEI	GCGC	1 cut(s)	74
AsuC2I	CCSGG	2 cut(s)	582, 1367
AsuHPI	GGTGA	9 cut(s)	56, 97, 377, 392, 1696, 1772, 1790, 2354, 2529
AsuNHI	GCTAGC	1 cut(s)	1565
AvaI	CYCGRG	2 cut(s)	2833, 2883
AvaII	GGWCC	7 cut(s)	614, 773, 1194, 1324, 2122, 2307, 2760
BaeGI	GKGCMC	3 cut(s)	481, 667, 2853
BalI	TGGCCA	3 cut(s)	1787, 1896, 2431
BamHI	GGATCC	1 cut(s)	2677
BanI	GGYRCC	1 cut(s)	664
BanII	GRGCYC	1 cut(s)	481
BbsI	GAAGAC	1 cut(s)	2198
BbvI	GCAGC	4 cut(s)	748, 1719, 2008, 2479
BceAI	ACGGC	1 cut(s)	81
BcgI	CGANNNNNNTGC	2 cut(s)	1065, 1099
BciT130I	CCWGG	6 cut(s)	236, 295, 1198, 1500, 2616, 2661
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	2 cut(s)	582, 1367
BfaI	CTAG	7 cut(s)	336, 590, 768, 828, 873, 1566, 2757
BfmI	CTRYAG	3 cut(s)	463, 572, 2211
BfuAI	ACCTGC	1 cut(s)	1163
BglII	AGATCT	1 cut(s)	2813
BisI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2493
BlsI	GCNGC	6 cut(s)	71, 349, 738, 1709, 2023, 2494
BmcAI	AGTACT	1 cut(s)	766
Bme1390I	CCNGG	8 cut(s)	236, 295, 582, 1198, 1367, 1500, 2616, 2661
Bme18I	GGWCC	7 cut(s)	614, 773, 1194, 1324, 2122, 2307, 2760
BmeT110I	CYCGRG	2 cut(s)	2833, 2883
BmrFI	CCNGG	8 cut(s)	236, 295, 582, 1198, 1367, 1500, 2616, 2661
BmrI	ACTGGG	4 cut(s)	1306, 1624, 1886, 2607
BmtI	GCTAGC	1 cut(s)	1569
BmuI	ACTGGG	4 cut(s)	1306, 1624, 1886, 2607
BpiI	GAAGAC	1 cut(s)	2198
BpuEI	CTTGAG	4 cut(s)	761, 1531, 1726, 2050
BpuMI	CCSGG	2 cut(s)	582, 1367
BsaAI	YACGTR	1 cut(s)	2522
BsaBI	GATNNNNATC	1 cut(s)	924
BsaHI	GRCGYC	2 cut(s)	2243, 2619
BsaJI	CCNNGG	5 cut(s)	234, 619, 2614, 2660, 2882
BsaWI	WCCGGW	3 cut(s)	424, 1644, 2304
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	8 cut(s)	241, 490, 713, 1321, 1717, 1794, 2385, 2725
Bse1I	ACTGG	8 cut(s)	571, 1301, 1619, 1840, 1881, 1927, 2602, 2679
Bse3DI	GCAATG	2 cut(s)	188, 288
Bse8I	GATNNNNATC	1 cut(s)	924
BseBI	CCWGG	6 cut(s)	236, 295, 1198, 1500, 2616, 2661
BseDI	CCNNGG	5 cut(s)	234, 619, 2614, 2660, 2882
BseJI	GATNNNNATC	1 cut(s)	924
BseLI	CCNNNNNNNGG	8 cut(s)	241, 490, 713, 1321, 1717, 1794, 2385, 2725
BseMI	GCAATG	2 cut(s)	188, 288
BseMII	CTCAG	5 cut(s)	530, 689, 2486, 2543, 2715
BseNI	ACTGG	8 cut(s)	571, 1301, 1619, 1840, 1881, 1927, 2602, 2679
BseRI	GAGGAG	1 cut(s)	205
BseSI	GKGCMC	3 cut(s)	481, 667, 2853
BseXI	GCAGC	4 cut(s)	748, 1719, 2008, 2479
BsgI	GTGCAG	4 cut(s)	260, 574, 645, 755
Bsh1236I	CGCG	3 cut(s)	72, 1062, 2580
BshFI	GGCC	9 cut(s)	479, 1413, 1462, 1787, 1896, 1959, 2397, 2431, 2602
BshNI	GGYRCC	1 cut(s)	664
BsiHKCI	CYCGRG	2 cut(s)	2833, 2883
BsiSI	CCGG	6 cut(s)	425, 581, 1366, 1645, 2289, 2305
BsiWI	CGTACG	1 cut(s)	2581
BslFI	GGGAC	7 cut(s)	600, 634, 1207, 1310, 2048, 2113, 2597
BslI	CCNNNNNNNGG	8 cut(s)	241, 490, 713, 1321, 1717, 1794, 2385, 2725
BsmFI	GGGAC	7 cut(s)	600, 634, 1207, 1310, 2048, 2113, 2597
BsmI	GAATGC	2 cut(s)	266, 1076
BsnI	GGCC	9 cut(s)	479, 1413, 1462, 1787, 1896, 1959, 2397, 2431, 2602
BsoBI	CYCGRG	2 cut(s)	2833, 2883
Bsp120I	GGGCCC	1 cut(s)	477
Bsp1286I	GDGCHC	3 cut(s)	481, 667, 2853
Bsp1407I	TGTACA	1 cut(s)	2779
BspANI	GGCC	9 cut(s)	479, 1413, 1462, 1787, 1896, 1959, 2397, 2431, 2602
BspCNI	CTCAG	5 cut(s)	529, 688, 2485, 2544, 2716
BspFNI	CGCG	3 cut(s)	72, 1062, 2580
BspHI	TCATGA	3 cut(s)	2155, 2181, 2647
BspMAI	CTGCAG	1 cut(s)	576
BspMI	ACCTGC	1 cut(s)	1163
BspOI	GCTAGC	1 cut(s)	1569
BspPI	GGATC	5 cut(s)	857, 2007, 2083, 2672, 2685
BspT107I	GGYRCC	1 cut(s)	664
BsrDI	GCAATG	2 cut(s)	188, 288
BsrGI	TGTACA	1 cut(s)	2779
BsrI	ACTGG	8 cut(s)	571, 1301, 1619, 1840, 1881, 1927, 2602, 2679
BssECI	CCNNGG	5 cut(s)	234, 619, 2614, 2660, 2882
BssNAI	GTATAC	1 cut(s)	820
BssNI	GRCGYC	2 cut(s)	2243, 2619
BssT1I	CCWWGG	1 cut(s)	619
Bst1107I	GTATAC	1 cut(s)	820
Bst2UI	CCWGG	6 cut(s)	236, 295, 1198, 1500, 2616, 2661
Bst4CI	ACNGT	5 cut(s)	43, 50, 1045, 2137, 2848
Bst6I	CTCTTC	6 cut(s)	223, 510, 1908, 2471, 2539, 2804
BstACI	GRCGYC	2 cut(s)	2243, 2619
BstAPI	GCANNNNNTGC	2 cut(s)	1349, 1697
BstAUI	TGTACA	1 cut(s)	2779
BstBAI	YACGTR	1 cut(s)	2522
BstC8I	GCNNGC	9 cut(s)	1099, 1158, 1567, 1571, 1986, 2429, 2604, 2716, 2853
BstDEI	CTNAG	6 cut(s)	516, 675, 724, 2472, 2552, 2724
BstENI	CCTNNNNNAGG	1 cut(s)	1715
BstFNI	CGCG	3 cut(s)	72, 1062, 2580
BstHHI	GCGC	1 cut(s)	74
BstMWI	GCNNNNNNNGC	5 cut(s)	476, 521, 1349, 1697, 2838
BstNI	CCWGG	6 cut(s)	236, 295, 1198, 1500, 2616, 2661
BstNSI	RCATGY	1 cut(s)	1551
BstSCI	CCNGG	8 cut(s)	234, 293, 580, 1196, 1365, 1498, 2614, 2659
BstSFI	CTRYAG	3 cut(s)	463, 572, 2211
BstSLI	GKGCMC	3 cut(s)	481, 667, 2853
BstSNI	TACGTA	1 cut(s)	2522
BstUI	CGCG	3 cut(s)	72, 1062, 2580
BstV1I	GCAGC	4 cut(s)	748, 1719, 2008, 2479
BstV2I	GAAGAC	1 cut(s)	2198
BstX2I	RGATCY	2 cut(s)	2677, 2813
BstXI	CCANNNNNNTGG	3 cut(s)	783, 945, 2597
BstYI	RGATCY	2 cut(s)	2677, 2813
BstZ17I	GTATAC	1 cut(s)	820
BsuRI	GGCC	9 cut(s)	479, 1413, 1462, 1787, 1896, 1959, 2397, 2431, 2602
BtgZI	GCGATG	2 cut(s)	1797, 2307
BtsI	GCAGTG	3 cut(s)	562, 633, 1560
BtsIMutI	CAGTG	7 cut(s)	562, 633, 1033, 1050, 1521, 1560, 1612
BveI	ACCTGC	1 cut(s)	1163
Cac8I	GCNNGC	9 cut(s)	1099, 1158, 1567, 1571, 1986, 2429, 2604, 2716, 2853
CaiI	CAGNNNCTG	2 cut(s)	686, 2615
CciI	TCATGA	3 cut(s)	2155, 2181, 2647
CfoI	GCGC	1 cut(s)	74
CseI	GACGC	2 cut(s)	2232, 2608
Csp6I	GTAC	7 cut(s)	12, 765, 1303, 1833, 2530, 2582, 2780
CspCI	CAANNNNNGTGG	4 cut(s)	1780, 1810, 1815, 1845
CviQI	GTAC	7 cut(s)	12, 765, 1303, 1833, 2530, 2582, 2780
DdeI	CTNAG	6 cut(s)	516, 675, 724, 2472, 2552, 2724
EaeI	YGGCCR	3 cut(s)	1785, 1894, 2429
Eam1104I	CTCTTC	6 cut(s)	223, 510, 1908, 2471, 2539, 2804
EarI	CTCTTC	6 cut(s)	223, 510, 1908, 2471, 2539, 2804
EciI	GGCGGA	3 cut(s)	21, 136, 913
Eco105I	TACGTA	1 cut(s)	2522
Eco130I	CCWWGG	1 cut(s)	619
Eco24I	GRGCYC	1 cut(s)	481
Eco32I	GATATC	1 cut(s)	1070
Eco47I	GGWCC	7 cut(s)	614, 773, 1194, 1324, 2122, 2307, 2760
Eco57I	CTGAAG	3 cut(s)	1221, 1889, 2133
Eco88I	CYCGRG	2 cut(s)	2833, 2883
EcoNI	CCTNNNNNAGG	1 cut(s)	1715
EcoO109I	RGGNCCY	5 cut(s)	477, 478, 1194, 1324, 2760
EcoRI	GAATTC	1 cut(s)	2271
EcoRII	CCWGG	6 cut(s)	234, 293, 1196, 1498, 2614, 2659
EcoRV	GATATC	1 cut(s)	1070
EcoT14I	CCWWGG	1 cut(s)	619
EcoT22I	ATGCAT	1 cut(s)	1813
EcoT38I	GRGCYC	1 cut(s)	481
ErhI	CCWWGG	1 cut(s)	619
FalI	AAGNNNNNCTT	4 cut(s)	384, 416, 1686, 1718
FaqI	GGGAC	7 cut(s)	600, 634, 1207, 1310, 2048, 2113, 2597
FauI	CCCGC	2 cut(s)	144, 2860
FauNDI	CATATG	3 cut(s)	868, 2092, 2113
FbaI	TGATCA	1 cut(s)	925
FblI	GTMKAC	3 cut(s)	819, 1437, 1927
Fnu4HI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2493
FriOI	GRGCYC	1 cut(s)	481
Fsp4HI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2493
FspBI	CTAG	7 cut(s)	336, 590, 768, 828, 873, 1566, 2757
GlaI	GCGC	1 cut(s)	73
GluI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2493
HaeIII	GGCC	9 cut(s)	479, 1413, 1462, 1787, 1896, 1959, 2397, 2431, 2602
HapII	CCGG	6 cut(s)	425, 581, 1366, 1645, 2289, 2305
HgaI	GACGC	2 cut(s)	2232, 2608
HhaI	GCGC	1 cut(s)	74
Hin1I	GRCGYC	2 cut(s)	2243, 2619
Hin6I	GCGC	1 cut(s)	72
HinP1I	GCGC	1 cut(s)	72
HincII	GTYRAC	1 cut(s)	46
HindII	GTYRAC	1 cut(s)	46
HindIII	AAGCTT	1 cut(s)	247
HpaII	CCGG	6 cut(s)	425, 581, 1366, 1645, 2289, 2305
HphI	GGTGA	9 cut(s)	56, 97, 377, 392, 1696, 1772, 1790, 2354, 2529
Hpy166II	GTNNAC	5 cut(s)	46, 820, 1438, 1555, 1928
Hpy188I	TCNGA	8 cut(s)	925, 991, 1240, 1249, 2080, 2475, 2512, 2553
Hpy8I	GTNNAC	5 cut(s)	46, 820, 1438, 1555, 1928
Hpy99I	CGWCG	1 cut(s)	102
HpyCH4III	ACNGT	5 cut(s)	43, 50, 1045, 2137, 2848
HpyCH4IV	ACGT	3 cut(s)	1978, 2521, 2584
HpyF10VI	GCNNNNNNNGC	5 cut(s)	476, 521, 1349, 1697, 2838
HpyF3I	CTNAG	6 cut(s)	516, 675, 724, 2472, 2552, 2724
HpySE526I	ACGT	3 cut(s)	1978, 2521, 2584
Hsp92I	GRCGYC	2 cut(s)	2243, 2619
HspAI	GCGC	1 cut(s)	72
KflI	GGGWCCC	1 cut(s)	1324
Ksp22I	TGATCA	1 cut(s)	925
LmnI	GCTCC	2 cut(s)	703, 2307
Lsp1109I	GCAGC	4 cut(s)	748, 1719, 2008, 2479
MaeI	CTAG	7 cut(s)	336, 590, 768, 828, 873, 1566, 2757
MaeII	ACGT	3 cut(s)	1978, 2521, 2584
MaeIII	GTNAC	7 cut(s)	576, 1502, 1918, 1946, 2324, 2480, 2517
MfeI	CAATTG	3 cut(s)	1371, 2383, 2768
MflI	RGATCY	2 cut(s)	2677, 2813
MhlI	GDGCHC	3 cut(s)	481, 667, 2853
MlsI	TGGCCA	3 cut(s)	1787, 1896, 2431
MluI	ACGCGT	1 cut(s)	2578
MluNI	TGGCCA	3 cut(s)	1787, 1896, 2431
MlyI	GAGTC	5 cut(s)	206, 1085, 2188, 2321, 2488
MmeI	TCCRAC	2 cut(s)	62, 2403
Mox20I	TGGCCA	3 cut(s)	1787, 1896, 2431
Mph1103I	ATGCAT	1 cut(s)	1813
MroXI	GAANNNNTTC	2 cut(s)	316, 1230
MscI	TGGCCA	3 cut(s)	1787, 1896, 2431
MseI	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2466, 2787
MslI	CAYNNNNRTG	5 cut(s)	1449, 1455, 2160, 2487, 2627
Msp20I	TGGCCA	3 cut(s)	1787, 1896, 2431
MspI	CCGG	6 cut(s)	425, 581, 1366, 1645, 2289, 2305
MspR9I	CCNGG	8 cut(s)	236, 295, 582, 1198, 1367, 1500, 2616, 2661
MunI	CAATTG	3 cut(s)	1371, 2383, 2768
Mva1269I	GAATGC	2 cut(s)	266, 1076
MvaI	CCWGG	6 cut(s)	236, 295, 1198, 1500, 2616, 2661
MvnI	CGCG	3 cut(s)	72, 1062, 2580
MwoI	GCNNNNNNNGC	5 cut(s)	476, 521, 1349, 1697, 2838
NciI	CCSGG	2 cut(s)	582, 1367
NdeI	CATATG	3 cut(s)	868, 2092, 2113
NheI	GCTAGC	1 cut(s)	1565
NmeAIII	GCCGAG	1 cut(s)	1073
NmuCI	GTSAC	3 cut(s)	1918, 2324, 2480
NsiI	ATGCAT	1 cut(s)	1813
NspI	RCATGY	1 cut(s)	1551
PagI	TCATGA	3 cut(s)	2155, 2181, 2647
PaqCI	CACCTGC	1 cut(s)	1163
PciI	ACATGT	1 cut(s)	1547
PctI	GAATGC	2 cut(s)	266, 1076
PdmI	GAANNNNTTC	2 cut(s)	316, 1230
PfeI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 1991
Pfl23II	CGTACG	1 cut(s)	2581
PflMI	CCANNNNNTGG	2 cut(s)	713, 2385
PkrI	GCNGC	6 cut(s)	71, 349, 738, 1709, 2023, 2494
PleI	GAGTC	5 cut(s)	206, 1085, 2188, 2321, 2487
PpsI	GAGTC	5 cut(s)	206, 1085, 2188, 2321, 2487
Ppu21I	YACGTR	1 cut(s)	2522
PpuMI	RGGWCCY	3 cut(s)	1194, 1324, 2760
PscI	ACATGT	1 cut(s)	1547
PsiI	TTATAA	1 cut(s)	2373
Psp5II	RGGWCCY	3 cut(s)	1194, 1324, 2760
Psp6I	CCWGG	6 cut(s)	234, 293, 1196, 1498, 2614, 2659
PspGI	CCWGG	6 cut(s)	234, 293, 1196, 1498, 2614, 2659
PspLI	CGTACG	1 cut(s)	2581
PspOMI	GGGCCC	1 cut(s)	477
PspPPI	RGGWCCY	3 cut(s)	1194, 1324, 2760
PstI	CTGCAG	1 cut(s)	576
PstNI	CAGNNNCTG	2 cut(s)	686, 2615
PsuI	RGATCY	2 cut(s)	2677, 2813
RsaI	GTAC	7 cut(s)	13, 766, 1304, 1834, 2531, 2583, 2781
RsaNI	GTAC	7 cut(s)	12, 765, 1303, 1833, 2530, 2582, 2780
RseI	CAYNNNNRTG	5 cut(s)	1449, 1455, 2160, 2487, 2627
SaqAI	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2466, 2787
SatI	GCNGC	6 cut(s)	70, 348, 737, 1708, 2022, 2493
ScaI	AGTACT	1 cut(s)	766
SchI	GAGTC	5 cut(s)	206, 1085, 2188, 2321, 2488
ScrFI	CCNGG	8 cut(s)	236, 295, 582, 1198, 1367, 1500, 2616, 2661
SduI	GDGCHC	3 cut(s)	481, 667, 2853
SfcI	CTRYAG	3 cut(s)	463, 572, 2211
SinI	GGWCC	7 cut(s)	614, 773, 1194, 1324, 2122, 2307, 2760
SmiMI	CAYNNNNRTG	5 cut(s)	1449, 1455, 2160, 2487, 2627
SmlI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SmoI	CTYRAG	4 cut(s)	740, 1510, 1741, 2029
SnaBI	TACGTA	1 cut(s)	2522
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	7 cut(s)	336, 590, 768, 828, 873, 1566, 2757
StyD4I	CCNGG	8 cut(s)	234, 293, 580, 1196, 1365, 1498, 2614, 2659
StyI	CCWWGG	1 cut(s)	619
TaaI	ACNGT	5 cut(s)	43, 50, 1045, 2137, 2848
TaiI	ACGT	3 cut(s)	1981, 2524, 2587
TaqI	TCGA	2 cut(s)	1858, 2877
TatI	WGTACW	3 cut(s)	11, 764, 2779
TauI	GCSGC	2 cut(s)	72, 350
TfiI	GAWTC	8 cut(s)	281, 317, 920, 1211, 1219, 1241, 1641, 1991
Tru1I	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2466, 2787
Tru9I	TTAA	9 cut(s)	359, 750, 1401, 1592, 1629, 1851, 2139, 2466, 2787
TscAI	CASTG	7 cut(s)	562, 633, 1033, 1050, 1528, 1567, 1619
TseFI	GTSAC	3 cut(s)	1918, 2324, 2480
TseI	GCWGC	4 cut(s)	736, 1707, 2021, 2492
Tsp45I	GTSAC	3 cut(s)	1918, 2324, 2480
TspGWI	ACGGA	1 cut(s)	2537
TspRI	CASTG	7 cut(s)	562, 633, 1033, 1050, 1528, 1567, 1619
Van91I	CCANNNNNTGG	2 cut(s)	713, 2385
VpaK11BI	GGWCC	7 cut(s)	614, 773, 1194, 1324, 2122, 2307, 2760
XagI	CCTNNNNNAGG	1 cut(s)	1715
XapI	RAATTY	6 cut(s)	142, 1055, 1474, 2205, 2271, 2503
XceI	RCATGY	1 cut(s)	1551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1283
XmiI	GTMKAC	3 cut(s)	819, 1437, 1927
XmnI	GAANNNNTTC	2 cut(s)	316, 1230
XspI	CTAG	7 cut(s)	336, 590, 768, 828, 873, 1566, 2757
ZrmI	AGTACT	1 cut(s)	766
Zsp2I	ATGCAT	1 cut(s)	1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.