Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0004764

Physical Location & Seq

Genomic Coordinates Reverse (-)

54458521 .. 54469716

Gene Structure 11196 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G027110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGCCTTTATGTACTTGTTCTCCGCTTCTTCCTCCTCCTCCTCCAACGGTCAACAGTCATTCCCTTCCTCACCGCCGCGCCTATTCTTCATCCTCCATACGCCGTCGTTTCACCTCCCAAGCCAAACCCATTTTCTCCTTCCCGCCAAATTCCGCCAAGTTTTCCACAACACCGCTACTTCAGTGCTCGTCCTCATTGCCCATGACTGCCGAACAAGACTCCTCTTCCGCCTCACATCCCCAGGTGCAGGGAAGCTTGTTGAAATCAAGGGCATTCTGGGTTAGTGAATCAATCATTGCCTGGAATGTGGATGCTGGAAATGATTCGTGCTACTTATTCGCTAGTGGAACTGCAGCGTTGTCATTAACGGGTGATGGAGTTTTGGGTGAGGATGTAAAAGTTCAGCTTCAAGAGGATAGACATGGGCTGCCGGAAAATGTGAAAACAAAGTTTCCTCATATCAAAGACTACAGAGCGTTTAGGGTCCCCCCTGATCTGGATGTCAGACCTCTTCTCAAAGCTCAGTTGGCAGTTGCTACTTTCAGTTCTGATGGAAGGTGCAGCGATGCTACTGGTCTGCAGTTACCGGGTGTTCTAGATGAATTGTTCTCGTATGATGGTTCCCTTGGTGCAGTGTATTCAAAAGAAGCTGTGTCCCTTTACCTTTGGGCACCTACTTCTCAGGCAGTATCTGTATTGATCTATAAGGAGCCATCGGGTGGAAATCCCTTAGAAATTGTGCAGCTTGAGGAAGTTAATGGTATTTGGAGTACTAGAGGACCAAAAACTTGGGAAGGATGCTACTATGTGTATGAAGTGTCTGTATACCACCCTAGCACCTTACAAATTGAAAAGTGCTATGCAAATGATCCATATGCTAGAGGGCTCTCAGCAGATGGTAGGCGGACATTGTTCATCAATCTTGATTCTGATCATATAAAACCAGAAGGATGGGATAAATTGGTAGATGAGAAACCTGATATACATTCTTCTTCGGACATAAGTATTTACGAGTTGCACATAAGAGATTTCAGTGCCAATGACCAGACAGTGCTTCCTGAATTTCGTGGTGGATATCTGGCATTCACTTCGCAGGACTCGGCTGGCGTATCTCATTTGAAGAAATTATCAAATGCTGGTATCACTCATGTCCATCTACTGCCAGCCTACCAATTTGCAGGTGTTGATGATGAGAAGGGGAACTGGAAGAGTGTAGATTCCAAGATTCTTGAAAAGTTTCCTTCAGATTCATCCGAGCAACAAGCTCTAATTACAGCAATCCAAAATGATGATGGGTACAACTGGGGGTACAACCCTGTCCTTTGGGGGGTCCCTAAAGGAAGCTATGCAAGTAATGCAAATGGTTCATACCGGGCAATTGAGTTTAGGAAGATGGTTCAGGCACTTAATCGTTTTGGCCTTCGTGTTGTCTTGGATGTTGTCTACAATCATTTGCATGGAAGTGGGCCATTTGATGAAAATTCAGTTCTTGATAAGATTGTTCCAGGTTACTACTTGAGAAGAAACACTGATGGTTTTATTGAGAATAGCACATGTGTAAACAACACTGCTAGCGAGCATTTCATGGTAGAGCGTTTAATTCTTGATGATCTTTTGCACTGGGCAGTTGATTTTAAGGTTGATGGATTCCGGTTTGACCTTATGGGTCATATAATGAAAAGAACAATGGTGAAAGCAAAAGATGCGCTTTGCAGCCTAACAAAGGATAGGGATGGAGTTGATGGCTCAAGTATCTATATATATGGTGAAGGATGGGATTTTGGTGAAGTGGCCAACAATGGGCGTGGAGTGAATGCATCGCAGTTCAATATTTGTGGTACTGGAATTGGGAGTTTTAATGATCGAATACGGGATGCAATACTGGGTGGTTCTCCATTTGGCCATCCTCTTCAGCAAGGATTTGTGACTGGTCTACTATTACAGCCTAATGGTTACGATCATGGCCCAGAAGTTGTTGCAGAATGTATGCTTGCCGAATCAAAGGATCACATTCAGGTTGGTATGGCTGCAAACTTGAGGGACTTTGTGCTAACCAATTATGAAGGGAAAGAGGTAAAAGGATCAGAAGTATTGACATATGGTGGGACAAGTGTAGCATATGCTTTGGACCCTACTGAAACTGTTAATTATGCTTCAGCTCATGACAACGAAACTCTTTTTGACATCGTGAGTCTGAAGACTCCATTTGAAATTTCTGTCGAAGAGAGATGCAGGATAAATCTCTTGGCGTCAAGCATAATAGCACTATCACAGGGAATTCCATTTTTCCACTCCGGTGATGAGATGCTCCGGTCCAAATCACTTGATCGCGACTCATACAATTCTGGTGATTGGTTCAACAGGATTGACTTCACTTATAATTCCAACAATTGGGGTGTTGGCCTTCCTCCAAAGGAGAAAAATGAAAAGAGCTGGCCACTAATGAAACCAAGATTAGCAGAGCCATCCTTTAAGCCTCAGAAGAGTCACATCCATGCTGCTGTGGAGAATTTTTCAGATTTGTTACATATTAGGTACTCTTCACCACTTTTCCGTCTGAGGACAGCAAATGCTATTCAGGTGAGGGGCTTCCTACACACCCTTATTTCTCCCTATAGTATATGTCCAATATGGCCAACTCCTGGTAGAAAATATTATGCTCTGAATGACTTTTTATATTGGGTTAAAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009250 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010303 GO:0016051 GO:0016052 GO:0016787 GO:0016798 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051060 GO:0071704 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_48 DUF3372

Protein Analysis

897

Amino Acids

99.63

Weight (kDa)

5.78

Isoelectric Point (pI)

38.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pullulanase_N2	PF17967	88 - 201	2.7e-33	Pullulanase N2 domain
CBM_48	PF02922	209 - 293	1.6e-06	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Pullul_strch_C	PF11852	793 - 862	3.1e-26	Alpha-1,6-glucosidases, pullulanase-type, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04360 AT5G04360
fragaria_vesca	FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370 FvH4_7g18370
malus_domestica	MD01G1100100.v1.1
prunus_persica	Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1 Prupe.2G205800_v2.0.a1
pyrus_communis	pycom01g12720
rosa_chinensis	RchiOBHm_Chr1g0362181
rosa_laevigata	RLG00000027669
rosa_multiflora	Rmu_sc0002133.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294460
rosa_rugosa	Rorug01G0294900 Rorug01G0295000 Rorug01G0295100 Rorug01G0295200
rosa_samantha	Rh1AG304800 Rh1BG267600 Rh1CG285800 Rh1DG297600
rosa_wichuraiana	Rw1G027110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2379
AarI	CACCTGC	1 cut(s)	1169
Acc36I	ACCTGC	1 cut(s)	1169
AccB1I	GGYRCC	1 cut(s)	670
AccB7I	CCANNNNNTGG	3 cut(s)	719, 2391, 2642
AccI	GTMKAC	3 cut(s)	825, 1443, 1933
AccII	CGCG	2 cut(s)	78, 2331
AciI	CCGC	8 cut(s)	23, 73, 76, 143, 153, 173, 228, 904
AclWI	GGATC	3 cut(s)	863, 2013, 2089
AcoI	YGGCCR	4 cut(s)	1791, 1900, 2435, 2633
AcsI	RAATTY	6 cut(s)	148, 1061, 1480, 2211, 2277, 2509
AcuI	CTGAAG	5 cut(s)	164, 1227, 1895, 2139, 2216
AcyI	GRCGYC	1 cut(s)	2249
AfaI	GTAC	6 cut(s)	13, 772, 1298, 1310, 1840, 2537
AfiI	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2642
AflIII	ACRYGT	1 cut(s)	1553
AgsI	TTSAA	9 cut(s)	262, 410, 642, 851, 1120, 1232, 1828, 2210, 2359
AjnI	CCWGG	4 cut(s)	240, 299, 1504, 2641
AjuI	GAANNNNNNNTTGG	2 cut(s)	116, 148
AleI	CACNNNNGTG	1 cut(s)	2295
AluBI	AGCT	9 cut(s)	255, 406, 521, 650, 745, 1265, 1344, 2159, 2433
AluI	AGCT	9 cut(s)	255, 406, 521, 650, 745, 1265, 1344, 2159, 2433
Alw21I	GWGCWC	1 cut(s)	188
AlwI	GGATC	3 cut(s)	863, 2013, 2089
AlwNI	CAGNNNCTG	1 cut(s)	692
AoxI	GGCC	8 cut(s)	1417, 1466, 1791, 1900, 1963, 2401, 2435, 2633
ApeKI	GCWGC	7 cut(s)	353, 427, 561, 742, 1713, 2027, 2498
ApoI	RAATTY	6 cut(s)	148, 1061, 1480, 2211, 2277, 2509
Asp700I	GAANNNNTTC	2 cut(s)	322, 1236
AspLEI	GCGC	2 cut(s)	80, 1708
AspS9I	GGNCC	7 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313
AsuC2I	CCSGG	2 cut(s)	588, 1373
AsuNHI	GCTAGC	1 cut(s)	1571
AvaII	GGWCC	5 cut(s)	484, 779, 1330, 2128, 2313
BaeGI	GKGCMC	1 cut(s)	673
BalI	TGGCCA	4 cut(s)	1793, 1902, 2437, 2635
BanI	GGYRCC	1 cut(s)	670
BanII	GRGCYC	1 cut(s)	888
BbsI	GAAGAC	1 cut(s)	2204
Bbv12I	GWGCWC	1 cut(s)	188
BbvI	GCAGC	7 cut(s)	365, 414, 573, 754, 1725, 2014, 2485
BceAI	ACGGC	1 cut(s)	87
BcgI	CGANNNNNNTGC	2 cut(s)	1071, 1105
BciT130I	CCWGG	4 cut(s)	242, 301, 1506, 2643
BclI	TGATCA	1 cut(s)	931
BcnI	CCSGG	2 cut(s)	588, 1373
BfaI	CTAG	6 cut(s)	342, 596, 774, 834, 879, 1572
BfmI	CTRYAG	4 cut(s)	351, 469, 578, 2614
BfuAI	ACCTGC	1 cut(s)	1169
BisI	GCNGC	8 cut(s)	76, 354, 428, 562, 743, 1714, 2028, 2499
BlsI	GCNGC	8 cut(s)	77, 355, 429, 563, 744, 1715, 2029, 2500
BmcAI	AGTACT	1 cut(s)	772
Bme1390I	CCNGG	6 cut(s)	242, 301, 588, 1373, 1506, 2643
Bme18I	GGWCC	5 cut(s)	484, 779, 1330, 2128, 2313
BmgT120I	GGNCC	7 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313
BmiI	GGNNCC	8 cut(s)	485, 486, 622, 672, 711, 1331, 1332, 2130
BmrFI	CCNGG	6 cut(s)	242, 301, 588, 1373, 1506, 2643
BmrI	ACTGGG	3 cut(s)	1312, 1630, 1892
BmsI	GCATC	8 cut(s)	301, 556, 788, 1693, 1826, 1864, 2219, 2295
BmtI	GCTAGC	1 cut(s)	1575
BmuI	ACTGGG	3 cut(s)	1312, 1630, 1892
BpiI	GAAGAC	1 cut(s)	2204
BpuEI	CTTGAG	4 cut(s)	767, 1537, 1732, 2056
BpuMI	CCSGG	2 cut(s)	588, 1373
BsaBI	GATNNNNATC	1 cut(s)	930
BsaHI	GRCGYC	1 cut(s)	2249
BsaJI	CCNNGG	2 cut(s)	240, 625
BsaWI	WCCGGW	3 cut(s)	1650, 2294, 2310
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2642
Bse1I	ACTGG	7 cut(s)	577, 1208, 1307, 1625, 1846, 1887, 1933
Bse3DI	GCAATG	2 cut(s)	194, 294
Bse8I	GATNNNNATC	1 cut(s)	930
BseBI	CCWGG	4 cut(s)	242, 301, 1506, 2643
BseDI	CCNNGG	2 cut(s)	240, 625
BseJI	GATNNNNATC	1 cut(s)	930
BseLI	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2642
BseMI	GCAATG	2 cut(s)	194, 294
BseMII	CTCAG	5 cut(s)	536, 695, 903, 2492, 2549
BseNI	ACTGG	7 cut(s)	577, 1208, 1307, 1625, 1846, 1887, 1933
BseRI	GAGGAG	4 cut(s)	24, 27, 30, 211
BseSI	GKGCMC	1 cut(s)	673
BseXI	GCAGC	7 cut(s)	365, 414, 573, 754, 1725, 2014, 2485
BsgI	GTGCAG	4 cut(s)	266, 580, 651, 761
Bsh1236I	CGCG	2 cut(s)	78, 2331
BshFI	GGCC	8 cut(s)	1419, 1468, 1793, 1902, 1965, 2403, 2437, 2635
BshNI	GGYRCC	1 cut(s)	670
BsiHKAI	GWGCWC	1 cut(s)	188
BsiSI	CCGG	6 cut(s)	431, 587, 1372, 1651, 2295, 2311
BslFI	GGGAC	5 cut(s)	470, 640, 1316, 2054, 2119
BslI	CCNNNNNNNGG	8 cut(s)	247, 496, 719, 1327, 1723, 1800, 2391, 2642
BsmFI	GGGAC	5 cut(s)	470, 640, 1316, 2054, 2119
BsmI	GAATGC	3 cut(s)	272, 1082, 1819
BsnI	GGCC	8 cut(s)	1419, 1468, 1793, 1902, 1965, 2403, 2437, 2635
Bsp1286I	GDGCHC	3 cut(s)	188, 673, 888
Bsp68I	TCGCGA	1 cut(s)	2331
BspACI	CCGC	8 cut(s)	23, 73, 76, 143, 153, 173, 228, 904
BspANI	GGCC	8 cut(s)	1419, 1468, 1793, 1902, 1965, 2403, 2437, 2635
BspCNI	CTCAG	5 cut(s)	535, 694, 902, 2491, 2550
BspFNI	CGCG	2 cut(s)	78, 2331
BspHI	TCATGA	1 cut(s)	2161
BspLI	GGNNCC	8 cut(s)	485, 486, 622, 672, 711, 1331, 1332, 2130
BspMAI	CTGCAG	2 cut(s)	355, 582
BspMI	ACCTGC	1 cut(s)	1169
BspOI	GCTAGC	1 cut(s)	1575
BspPI	GGATC	3 cut(s)	863, 2013, 2089
BspT107I	GGYRCC	1 cut(s)	670
BsrDI	GCAATG	2 cut(s)	194, 294
BsrI	ACTGG	7 cut(s)	577, 1208, 1307, 1625, 1846, 1887, 1933
BssECI	CCNNGG	2 cut(s)	240, 625
BssNAI	GTATAC	1 cut(s)	826
BssNI	GRCGYC	1 cut(s)	2249
BssT1I	CCWWGG	1 cut(s)	625
Bst1107I	GTATAC	1 cut(s)	826
Bst2UI	CCWGG	4 cut(s)	242, 301, 1506, 2643
Bst4CI	ACNGT	4 cut(s)	49, 56, 1051, 2143
Bst6I	CTCTTC	7 cut(s)	229, 516, 1202, 1914, 2217, 2477, 2545
BstACI	GRCGYC	1 cut(s)	2249
BstAPI	GCANNNNNTGC	2 cut(s)	1355, 1703
BstC8I	GCNNGC	6 cut(s)	1105, 1164, 1573, 1577, 1992, 2435
BstDEI	CTNAG	7 cut(s)	522, 681, 730, 889, 2478, 2558, 2691
BstENI	CCTNNNNNAGG	1 cut(s)	1721
BstFNI	CGCG	2 cut(s)	78, 2331
BstHHI	GCGC	2 cut(s)	80, 1708
BstMWI	GCNNNNNNNGC	3 cut(s)	527, 1355, 1703
BstNI	CCWGG	4 cut(s)	242, 301, 1506, 2643
BstNSI	RCATGY	1 cut(s)	1557
BstSCI	CCNGG	6 cut(s)	240, 299, 586, 1371, 1504, 2641
BstSFI	CTRYAG	4 cut(s)	351, 469, 578, 2614
BstSLI	GKGCMC	1 cut(s)	673
BstUI	CGCG	2 cut(s)	78, 2331
BstV1I	GCAGC	7 cut(s)	365, 414, 573, 754, 1725, 2014, 2485
BstV2I	GAAGAC	1 cut(s)	2204
BstXI	CCANNNNNNTGG	2 cut(s)	789, 951
BstZ17I	GTATAC	1 cut(s)	826
BsuRI	GGCC	8 cut(s)	1419, 1468, 1793, 1902, 1965, 2403, 2437, 2635
BtgZI	GCGATG	2 cut(s)	579, 1803
BtsI	GCAGTG	2 cut(s)	639, 1566
BtsIMutI	CAGTG	7 cut(s)	188, 639, 1039, 1056, 1527, 1566, 1618
BtuMI	TCGCGA	1 cut(s)	2331
BveI	ACCTGC	1 cut(s)	1169
Cac8I	GCNNGC	6 cut(s)	1105, 1164, 1573, 1577, 1992, 2435
CaiI	CAGNNNCTG	1 cut(s)	692
CciI	TCATGA	1 cut(s)	2161
CfoI	GCGC	2 cut(s)	80, 1708
Cfr13I	GGNCC	7 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313
CseI	GACGC	1 cut(s)	2238
Csp6I	GTAC	6 cut(s)	12, 771, 1297, 1309, 1839, 2536
CspCI	CAANNNNNGTGG	4 cut(s)	1786, 1816, 1821, 1851
CviAII	CATG	9 cut(s)	202, 422, 1148, 1457, 1554, 1585, 1961, 2162, 2495
CviQI	GTAC	6 cut(s)	12, 771, 1297, 1309, 1839, 2536
DdeI	CTNAG	7 cut(s)	522, 681, 730, 889, 2478, 2558, 2691
EaeI	YGGCCR	4 cut(s)	1791, 1900, 2435, 2633
Eam1104I	CTCTTC	7 cut(s)	229, 516, 1202, 1914, 2217, 2477, 2545
EarI	CTCTTC	7 cut(s)	229, 516, 1202, 1914, 2217, 2477, 2545
EciI	GGCGGA	3 cut(s)	142, 217, 919
Eco130I	CCWWGG	1 cut(s)	625
Eco24I	GRGCYC	1 cut(s)	888
Eco32I	GATATC	1 cut(s)	1076
Eco47I	GGWCC	5 cut(s)	484, 779, 1330, 2128, 2313
Eco57I	CTGAAG	5 cut(s)	164, 1227, 1895, 2139, 2216
EcoNI	CCTNNNNNAGG	1 cut(s)	1721
EcoO109I	RGGNCCY	2 cut(s)	484, 1330
EcoRI	GAATTC	1 cut(s)	2277
EcoRII	CCWGG	4 cut(s)	240, 299, 1504, 2641
EcoRV	GATATC	1 cut(s)	1076
EcoT14I	CCWWGG	1 cut(s)	625
EcoT22I	ATGCAT	1 cut(s)	1819
EcoT38I	GRGCYC	1 cut(s)	888
ErhI	CCWWGG	1 cut(s)	625
FaeI	CATG	9 cut(s)	205, 425, 1151, 1460, 1557, 1588, 1964, 2165, 2498
FalI	AAGNNNNNCTT	4 cut(s)	390, 422, 1692, 1724
FaqI	GGGAC	5 cut(s)	470, 640, 1316, 2054, 2119
FatI	CATG	9 cut(s)	201, 421, 1147, 1456, 1553, 1584, 1960, 2161, 2494
FauI	CCCGC	1 cut(s)	150
FauNDI	CATATG	3 cut(s)	874, 2098, 2119
FbaI	TGATCA	1 cut(s)	931
FblI	GTMKAC	3 cut(s)	825, 1443, 1933
Fnu4HI	GCNGC	8 cut(s)	76, 354, 428, 562, 743, 1714, 2028, 2499
FriOI	GRGCYC	1 cut(s)	888
Fsp4HI	GCNGC	8 cut(s)	76, 354, 428, 562, 743, 1714, 2028, 2499
FspBI	CTAG	6 cut(s)	342, 596, 774, 834, 879, 1572
GlaI	GCGC	2 cut(s)	79, 1707
GluI	GCNGC	8 cut(s)	76, 354, 428, 562, 743, 1714, 2028, 2499
HaeIII	GGCC	8 cut(s)	1419, 1468, 1793, 1902, 1965, 2403, 2437, 2635
HapII	CCGG	6 cut(s)	431, 587, 1372, 1651, 2295, 2311
HgaI	GACGC	1 cut(s)	2238
HhaI	GCGC	2 cut(s)	80, 1708
Hin1I	GRCGYC	1 cut(s)	2249
Hin1II	CATG	9 cut(s)	205, 425, 1151, 1460, 1557, 1588, 1964, 2165, 2498
Hin6I	GCGC	2 cut(s)	78, 1706
HinP1I	GCGC	2 cut(s)	78, 1706
HincII	GTYRAC	1 cut(s)	52
HindII	GTYRAC	1 cut(s)	52
HindIII	AAGCTT	1 cut(s)	253
HpaII	CCGG	6 cut(s)	431, 587, 1372, 1651, 2295, 2311
Hpy166II	GTNNAC	5 cut(s)	52, 826, 1444, 1561, 1934
Hpy8I	GTNNAC	5 cut(s)	52, 826, 1444, 1561, 1934
Hpy99I	CGWCG	1 cut(s)	108
HpyCH4III	ACNGT	4 cut(s)	49, 56, 1051, 2143
HpyF10VI	GCNNNNNNNGC	3 cut(s)	527, 1355, 1703
HpyF3I	CTNAG	7 cut(s)	522, 681, 730, 889, 2478, 2558, 2691
Hsp92I	GRCGYC	1 cut(s)	2249
Hsp92II	CATG	9 cut(s)	205, 425, 1151, 1460, 1557, 1588, 1964, 2165, 2498
HspAI	GCGC	2 cut(s)	78, 1706
KflI	GGGWCCC	2 cut(s)	484, 1330
Ksp22I	TGATCA	1 cut(s)	931
LmnI	GCTCC	2 cut(s)	709, 2313
Lsp1109I	GCAGC	7 cut(s)	365, 414, 573, 754, 1725, 2014, 2485
LweI	GCATC	8 cut(s)	301, 556, 788, 1693, 1826, 1864, 2219, 2295
MaeI	CTAG	6 cut(s)	342, 596, 774, 834, 879, 1572
MaeIII	GTNAC	6 cut(s)	582, 1508, 1924, 1952, 2486, 2523
MfeI	CAATTG	2 cut(s)	1377, 2389
MhlI	GDGCHC	3 cut(s)	188, 673, 888
MlsI	TGGCCA	4 cut(s)	1793, 1902, 2437, 2635
MluNI	TGGCCA	4 cut(s)	1793, 1902, 2437, 2635
MlyI	GAGTC	6 cut(s)	212, 1091, 2194, 2200, 2327, 2494
MmeI	TCCRAC	2 cut(s)	68, 2409
Mox20I	TGGCCA	4 cut(s)	1793, 1902, 2437, 2635
Mph1103I	ATGCAT	1 cut(s)	1819
MroXI	GAANNNNTTC	2 cut(s)	322, 1236
MscI	TGGCCA	4 cut(s)	1793, 1902, 2437, 2635
MseI	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2685
MslI	CAYNNNNRTG	4 cut(s)	1455, 1461, 2295, 2493
Msp20I	TGGCCA	4 cut(s)	1793, 1902, 2437, 2635
MspI	CCGG	6 cut(s)	431, 587, 1372, 1651, 2295, 2311
MspR9I	CCNGG	6 cut(s)	242, 301, 588, 1373, 1506, 2643
MunI	CAATTG	2 cut(s)	1377, 2389
Mva1269I	GAATGC	3 cut(s)	272, 1082, 1819
MvaI	CCWGG	4 cut(s)	242, 301, 1506, 2643
MvnI	CGCG	2 cut(s)	78, 2331
MwoI	GCNNNNNNNGC	3 cut(s)	527, 1355, 1703
NciI	CCSGG	2 cut(s)	588, 1373
NdeI	CATATG	3 cut(s)	874, 2098, 2119
NheI	GCTAGC	1 cut(s)	1571
NlaIII	CATG	9 cut(s)	205, 425, 1151, 1460, 1557, 1588, 1964, 2165, 2498
NlaIV	GGNNCC	8 cut(s)	485, 486, 622, 672, 711, 1331, 1332, 2130
NmeAIII	GCCGAG	1 cut(s)	1079
NmuCI	GTSAC	2 cut(s)	1924, 2486
NruI	TCGCGA	1 cut(s)	2331
NsiI	ATGCAT	1 cut(s)	1819
NspI	RCATGY	1 cut(s)	1557
OliI	CACNNNNGTG	1 cut(s)	2295
PagI	TCATGA	1 cut(s)	2161
PaqCI	CACCTGC	1 cut(s)	1169
PciI	ACATGT	1 cut(s)	1553
PctI	GAATGC	3 cut(s)	272, 1082, 1819
PdmI	GAANNNNTTC	2 cut(s)	322, 1236
PfeI	GAWTC	8 cut(s)	287, 323, 926, 1217, 1225, 1247, 1647, 1997
PflMI	CCANNNNNTGG	3 cut(s)	719, 2391, 2642
PkrI	GCNGC	8 cut(s)	77, 355, 429, 563, 744, 1715, 2029, 2500
PleI	GAGTC	6 cut(s)	212, 1091, 2194, 2199, 2327, 2493
PpsI	GAGTC	6 cut(s)	212, 1091, 2194, 2199, 2327, 2493
PpuMI	RGGWCCY	2 cut(s)	484, 1330
PscI	ACATGT	1 cut(s)	1553
PsiI	TTATAA	1 cut(s)	2379
Psp5II	RGGWCCY	2 cut(s)	484, 1330
Psp6I	CCWGG	4 cut(s)	240, 299, 1504, 2641
PspGI	CCWGG	4 cut(s)	240, 299, 1504, 2641
PspN4I	GGNNCC	8 cut(s)	485, 486, 622, 672, 711, 1331, 1332, 2130
PspPI	GGNCC	7 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313
PspPPI	RGGWCCY	2 cut(s)	484, 1330
PstI	CTGCAG	2 cut(s)	355, 582
PstNI	CAGNNNCTG	1 cut(s)	692
RruI	TCGCGA	1 cut(s)	2331
RsaI	GTAC	6 cut(s)	13, 772, 1298, 1310, 1840, 2537
RsaNI	GTAC	6 cut(s)	12, 771, 1297, 1309, 1839, 2536
RseI	CAYNNNNRTG	4 cut(s)	1455, 1461, 2295, 2493
SaqAI	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2685
SatI	GCNGC	8 cut(s)	76, 354, 428, 562, 743, 1714, 2028, 2499
Sau96I	GGNCC	7 cut(s)	484, 779, 1330, 1466, 1964, 2128, 2313
ScaI	AGTACT	1 cut(s)	772
SchI	GAGTC	6 cut(s)	212, 1091, 2194, 2200, 2327, 2494
ScrFI	CCNGG	6 cut(s)	242, 301, 588, 1373, 1506, 2643
SduI	GDGCHC	3 cut(s)	188, 673, 888
SfaNI	GCATC	8 cut(s)	301, 556, 788, 1693, 1826, 1864, 2219, 2295
SfcI	CTRYAG	4 cut(s)	351, 469, 578, 2614
SinI	GGWCC	5 cut(s)	484, 779, 1330, 2128, 2313
SmiMI	CAYNNNNRTG	4 cut(s)	1455, 1461, 2295, 2493
SmlI	CTYRAG	4 cut(s)	746, 1516, 1747, 2035
SmoI	CTYRAG	4 cut(s)	746, 1516, 1747, 2035
SsiI	CCGC	8 cut(s)	23, 73, 76, 143, 153, 173, 228, 904
SspI	AATATT	2 cut(s)	1831, 2654
SspMI	CTAG	6 cut(s)	342, 596, 774, 834, 879, 1572
StyD4I	CCNGG	6 cut(s)	240, 299, 586, 1371, 1504, 2641
StyI	CCWWGG	1 cut(s)	625
TaaI	ACNGT	4 cut(s)	49, 56, 1051, 2143
TaqI	TCGA	2 cut(s)	1864, 2220
TatI	WGTACW	2 cut(s)	11, 770
TauI	GCSGC	1 cut(s)	78
TfiI	GAWTC	8 cut(s)	287, 323, 926, 1217, 1225, 1247, 1647, 1997
Tru1I	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2685
Tru9I	TTAA	9 cut(s)	365, 756, 1407, 1598, 1635, 1857, 2145, 2472, 2685
TscAI	CASTG	7 cut(s)	188, 639, 1039, 1056, 1534, 1573, 1625
TseFI	GTSAC	2 cut(s)	1924, 2486
TseI	GCWGC	7 cut(s)	353, 427, 561, 742, 1713, 2027, 2498
Tsp45I	GTSAC	2 cut(s)	1924, 2486
TspGWI	ACGGA	1 cut(s)	2543
TspRI	CASTG	7 cut(s)	188, 639, 1039, 1056, 1534, 1573, 1625
Van91I	CCANNNNNTGG	3 cut(s)	719, 2391, 2642
VpaK11BI	GGWCC	5 cut(s)	484, 779, 1330, 2128, 2313
XagI	CCTNNNNNAGG	1 cut(s)	1721
XapI	RAATTY	6 cut(s)	148, 1061, 1480, 2211, 2277, 2509
XbaI	TCTAGA	1 cut(s)	595
XceI	RCATGY	1 cut(s)	1557
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1289
XmiI	GTMKAC	3 cut(s)	825, 1443, 1933
XmnI	GAANNNNTTC	2 cut(s)	322, 1236
XspI	CTAG	6 cut(s)	342, 596, 774, 834, 879, 1572
ZrmI	AGTACT	1 cut(s)	772
Zsp2I	ATGCAT	1 cut(s)	1819

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.