AT5G04520

Protein of unknown function (DUF455)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011094
Physical Location & Seq
Reverse (-)
1289885 .. 1291104
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04520.1

Sequence Viewer

Length: 876 bp
ATGGATTGCGAAACCCTAATTGAATCGGCGATCAGGATTCTGAACACATCAGACCCGCACGAAAAAGCACGACTTGGCGATTCGATAGCCGTGAAATGGCTTCAAGGTGCAATCGCCGAACCCTACGATCCCACCGTCGACTTCCCCGTCCCGGACAGACCGGCTCGACTTCCGGTGAAACTGGTGTCTCCAAGTTTGATGCCGAAGCTGGGAAGAGCTGGTAGCTTACAGAGCAGGCAAGCAATTGTTCATAGTCTTGCTCATACTGAAAGTTGGGCTATTGACTTATCTTGGGATATAATTGCTCGTTTTGGCAAGCAAGAGAAGATGCCAAGAGACTTCTTTACGGATTTTGTTCGTGTTGCTCAGGACGAAGGTCGTCACTTCACTTTGCTTGCAGCTCGGCTTGAGGAAATTGGTTCTAGTTACGGTGCATTGCCTGCTCATGACGGTCTTTGGGACTCTGCTACTGCCACATCTCACGATCTTTTGGCTCGTTTGGCTATAGAGCATTGCGTTCACGAGGCTAGAGGGCTTGATGTACTGCCCACAACGATATCTCGGTTTAGGAATGGAGGCGATAACGAGACAGCAGATTTACTAGAGAAAGTTGTGTACCCTGAAGAAATCACTCACTGTGCAGCTGGAGTAAAATGGTTTAAGTATCTATGTGAACGGTCCAAGGATCCTGAATTCACCATCAGCAGCAAAGAATCAGATGATAGCAATGAAGAGATAATCAACAAGTTTCATTCGGTTGTAAGGGAGCATTTCAGAGGACCGTTGAAACCGCCTTTTAATGCTGAAGCAAGAAAAGCTGCTGGCTTTGGTCCTCAATGGTATGAACCTCTTGCTGTCAAAGAGAGCAATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF455

Protein Analysis

291

Amino Acids

32.5

Weight (kDa)

5.59

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF455 PF04305 15 - 282 1e-89 Protein of unknown function (DUF455)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04520
fragaria_vesca FvH4_7g18310 FvH4_7g18310 FvH4_7g18310 FvH4_7g18310
malus_domestica MD01G1099600.v1.1
prunus_persica Prupe.2G205100_v2.0.a1
pyrus_communis pycom01g12670
rosa_chinensis RchiOBHm_Chr1g0362011
rosa_laevigata RLG00000027680
rosa_multiflora Rmu_co8465241.1_g000001 Rmu_sc0010614.1_g000009
rosa_roxburghii Rroxscaffold_4G00294590
rosa_rugosa Rorug01G0292500 Rorug01G0292600
rosa_samantha Rh1AG303000 Rh1BG266200 Rh1CG284400 Rh1DG296200
rosa_wichuraiana Rw1G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 138
AciI CCGC 2 cut(s) 56, 791
AclWI GGATC 3 cut(s) 122, 680, 693
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 2 cut(s) 642, 825
AfaI GTAC 2 cut(s) 543, 617
AfiI CCNNNNNNNGG 3 cut(s) 96, 151, 209
AgsI TTSAA 3 cut(s) 23, 104, 787
AluBI AGCT 6 cut(s) 208, 218, 225, 401, 644, 818
AluI AGCT 6 cut(s) 208, 218, 225, 401, 644, 818
Alw26I GTCTC 3 cut(s) 192, 330, 581
AlwI GGATC 3 cut(s) 122, 680, 693
ApeKI GCWGC 4 cut(s) 398, 641, 705, 818
ApoI RAATTY 1 cut(s) 692
AspS9I GGNCC 3 cut(s) 678, 779, 830
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 2 cut(s) 187, 688
AvaII GGWCC 3 cut(s) 678, 779, 830
BamHI GGATCC 1 cut(s) 685
BauI CACGAG 1 cut(s) 521
BbvI GCAGC 4 cut(s) 410, 653, 717, 805
BccI CCATC 1 cut(s) 707
BceAI ACGGC 1 cut(s) 74
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 3 cut(s) 192, 330, 581
BfaI CTAG 4 cut(s) 423, 528, 602, 874
BfmI CTRYAG 1 cut(s) 504
BisI GCNGC 4 cut(s) 399, 642, 706, 819
BlsI GCNGC 4 cut(s) 400, 643, 707, 820
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 3 cut(s) 678, 779, 830
BmgT120I GGNCC 3 cut(s) 678, 779, 830
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 189, 318
BoxI GACNNNNGTC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 666
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 681
BsaWI WCCGGW 1 cut(s) 172
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 151, 209
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 186
Bse3DI GCAATG 4 cut(s) 434, 511, 733, 874
BseDI CCNNGG 1 cut(s) 681
BseLI CCNNNNNNNGG 3 cut(s) 96, 151, 209
BseMI GCAATG 4 cut(s) 434, 511, 733, 874
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 186
BseXI GCAGC 4 cut(s) 410, 653, 717, 805
BseYI CCCAGC 1 cut(s) 208
BsgI GTGCAG 1 cut(s) 660
BsiSI CCGG 3 cut(s) 152, 161, 173
BslFI GGGAC 2 cut(s) 134, 473
BslI CCNNNNNNNGG 3 cut(s) 96, 151, 209
BsmAI GTCTC 3 cut(s) 192, 330, 581
BsmFI GGGAC 2 cut(s) 134, 473
Bsp143I GATC 4 cut(s) 30, 127, 484, 685
BspACI CCGC 2 cut(s) 56, 791
BspCNI CTCAG 1 cut(s) 379
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 687
BspPI GGATC 3 cut(s) 122, 680, 693
BspQI GCTCTTC 1 cut(s) 208
BsrDI GCAATG 4 cut(s) 434, 511, 733, 874
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 681
BssMI GATC 4 cut(s) 30, 127, 484, 685
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 521
Bst4CI ACNGT 6 cut(s) 136, 431, 452, 638, 678, 783
Bst6I CTCTTC 2 cut(s) 208, 726
BstAPI GCANNNNNTGC 1 cut(s) 440
BstC8I GCNNGC 6 cut(s) 236, 240, 317, 396, 441, 823
BstDEI CTNAG 1 cut(s) 366
BstKTI GATC 4 cut(s) 33, 130, 487, 688
BstMAI GTCTC 3 cut(s) 192, 330, 581
BstMBI GATC 4 cut(s) 30, 127, 484, 685
BstMWI GCNNNNNNNGC 4 cut(s) 231, 440, 500, 815
BstPAI GACNNNNGTC 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 4 cut(s) 410, 653, 717, 805
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BtsIMutI CAGTG 1 cut(s) 634
Cac8I GCNNGC 6 cut(s) 236, 240, 317, 396, 441, 823
CciI TCATGA 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 3 cut(s) 678, 779, 830
Csp6I GTAC 2 cut(s) 542, 616
CviAII CATG 1 cut(s) 446
CviQI GTAC 2 cut(s) 542, 616
DdeI CTNAG 1 cut(s) 366
DpnI GATC 4 cut(s) 32, 129, 486, 687
DpnII GATC 4 cut(s) 30, 127, 484, 685
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
Eam1104I CTCTTC 2 cut(s) 208, 726
EarI CTCTTC 2 cut(s) 208, 726
Eco130I CCWWGG 1 cut(s) 681
Eco32I GATATC 1 cut(s) 558
Eco47I GGWCC 3 cut(s) 678, 779, 830
Eco57I CTGAAG 2 cut(s) 642, 825
EcoRI GAATTC 1 cut(s) 692
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 1 cut(s) 449
FaiI YATR 7 cut(s) 252, 264, 299, 447, 506, 670, 843
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 2 cut(s) 134, 473
FatI CATG 1 cut(s) 445
FauI CCCGC 1 cut(s) 63
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 399, 642, 706, 819
Fsp4HI GCNGC 4 cut(s) 399, 642, 706, 819
FspBI CTAG 4 cut(s) 423, 528, 602, 874
GluI GCNGC 4 cut(s) 399, 642, 706, 819
GsaI CCCAGC 1 cut(s) 212
GsuI CTGGAG 1 cut(s) 666
HapII CCGG 3 cut(s) 152, 161, 173
Hin1II CATG 1 cut(s) 449
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 5 cut(s) 23, 37, 80, 461, 713
HpaII CCGG 3 cut(s) 152, 161, 173
HphI GGTGA 2 cut(s) 187, 688
Hpy166II GTNNAC 4 cut(s) 139, 520, 616, 674
Hpy188I TCNGA 4 cut(s) 42, 52, 718, 776
Hpy188III TCNNGA 6 cut(s) 34, 368, 446, 482, 521, 689
Hpy8I GTNNAC 4 cut(s) 139, 520, 616, 674
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 368
HpyCH4III ACNGT 6 cut(s) 136, 431, 452, 638, 678, 783
HpyCH4V TGCA 4 cut(s) 110, 398, 434, 641
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 440, 500, 815
HpyF3I CTNAG 1 cut(s) 366
Hsp92II CATG 1 cut(s) 449
Kzo9I GATC 4 cut(s) 30, 127, 484, 685
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 4 cut(s) 410, 653, 717, 805
LweI GCATC 2 cut(s) 189, 318
MaeI CTAG 4 cut(s) 423, 528, 602, 874
MaeIII GTNAC 2 cut(s) 380, 425
MalI GATC 4 cut(s) 32, 129, 486, 687
MboI GATC 4 cut(s) 30, 127, 484, 685
MboII GAAGA 4 cut(s) 225, 337, 635, 743
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 1 cut(s) 685
MluCI AATT 5 cut(s) 18, 243, 300, 414, 692
MlyI GAGTC 1 cut(s) 455
MnlI CCTC 7 cut(s) 403, 517, 524, 569, 770, 843, 858
MseI TTAA 2 cut(s) 660, 798
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 3 cut(s) 152, 161, 173
MspR9I CCNGG 1 cut(s) 152
MunI CAATTG 1 cut(s) 243
MwoI GCNNNNNNNGC 4 cut(s) 231, 440, 500, 815
NciI CCSGG 1 cut(s) 152
NdeII GATC 4 cut(s) 30, 127, 484, 685
NlaIII CATG 1 cut(s) 449
NlaIV GGNNCC 1 cut(s) 687
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 380
PagI TCATGA 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 144
PfeI GAWTC 4 cut(s) 23, 37, 80, 713
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 4 cut(s) 400, 643, 707, 820
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
PshAI GACNNNNGTC 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 3 cut(s) 678, 779, 830
PsuI RGATCY 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 543, 617
RsaNI GTAC 2 cut(s) 542, 616
SalI GTCGAC 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 660, 798
SatI GCNGC 4 cut(s) 399, 642, 706, 819
Sau3AI GATC 4 cut(s) 30, 127, 484, 685
Sau96I GGNCC 3 cut(s) 678, 779, 830
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 152
SetI ASST 9 cut(s) 109, 210, 220, 227, 379, 403, 646, 820, 850
SfaNI GCATC 2 cut(s) 189, 318
SfcI CTRYAG 1 cut(s) 504
SinI GGWCC 3 cut(s) 678, 779, 830
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 5 cut(s) 18, 243, 300, 414, 692
SsiI CCGC 2 cut(s) 56, 791
SspMI CTAG 4 cut(s) 423, 528, 602, 874
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 6 cut(s) 136, 431, 452, 638, 678, 783
TaqI TCGA 3 cut(s) 83, 138, 166
TasI AATT 5 cut(s) 18, 243, 300, 414, 692
TatI WGTACW 1 cut(s) 541
TfiI GAWTC 4 cut(s) 23, 37, 80, 713
Tru1I TTAA 2 cut(s) 660, 798
Tru9I TTAA 2 cut(s) 660, 798
TscAI CASTG 1 cut(s) 641
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 4 cut(s) 398, 641, 705, 818
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 4 cut(s) 239, 740, 744, 858
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 641
VpaK11BI GGWCC 3 cut(s) 678, 779, 830
XapI RAATTY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 4 cut(s) 423, 528, 602, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.