MD01G1099600.v1.1

Protein of unknown function (DUF455)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011094
Physical Location & Seq
Forward (+)
21280524 .. 21282935
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1099600.v1.1.491

Sequence Viewer

Length: 927 bp
ATGTGTGAAGACCGCTGCATACAAAAAAATATGAGCGGAACCGAGAGCTTAGTGGAAGCGGCGACAAGGGTATTGAACACGGCGGACCCATTCGAGAAGGCCCGAGTCGGTGACTCGGTGGCGGCTCAGTGGCTCGGAGGGACCATCACTCGTGCGTACGATTCGTCCCTGGACCTCTCCGTGCCTGATCGCCCCTCCAGGCTCAGCAATGTTAAGTTGGTGGCGCCGAGTTCGATGCCGAAGATTGGGAAAGGCGGGAGCTTAAGGAGTAGGCAGGCCATAGTTCATAGCCTCACGCACACTGAGAGTTGGGCTATTGACTTGTCTTGGGACATAATTGCTAGGTTCGGGAAGCAAGAGGCAATGCCGAGAGAGTTCTTCACGGATTTCGTGAAGGTAGCACGGGACGAAGGTCGTCACTTCACTCTGCTGGCATCTCGCCTGGAGGAGATGGGTTCCTTCTATGGAGCTTTTCCAGCCCATGACGGCCTCTGGGACTCGGCAACTGCCACTTCCAAAGACCTAATGGCGCGCCTTGCAATCGAGCATTGCGTCCACGAGGCAAGAGGACTCGACGTGCTCCCAACAACCATCTCTCGATTCCGCAGTGGAGGGGATGACAAAACGGCTGAGTTGCTGGAGAGCGTGGTGTACCCGGAAGAAATAACTCATTGTGCTGCTGGAGTTAAGTGGTTCAAGTATCTCTGCTTGAGGCCGGTGGACAGCGGTTCGGATCAACGGCGCTTGACGAACTTGGAGGCTGATGAAGAAGCTGAAGTGATTAAAAAGTTTCATGGAACTGTGAGAAGGTATTTTAGGGGGGTTTTGAAGCCTCCTTTCAATGAAAAGGCGAGGGAAATTGCTGGGTTTGGGTCTCAGTGGTATGAGCCTCTTACTGTCAAAGAGCAGGATACGCAGCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF455

Protein Analysis

309

Amino Acids

34.41

Weight (kDa)

6.17

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF455 PF04305 26 - 296 2.3e-86 Protein of unknown function (DUF455)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04520
fragaria_vesca FvH4_7g18310 FvH4_7g18310 FvH4_7g18310 FvH4_7g18310
malus_domestica MD01G1099600.v1.1
prunus_persica Prupe.2G205100_v2.0.a1
pyrus_communis pycom01g12670
rosa_chinensis RchiOBHm_Chr1g0362011
rosa_laevigata RLG00000027680
rosa_multiflora Rmu_co8465241.1_g000001 Rmu_sc0010614.1_g000009
rosa_roxburghii Rroxscaffold_4G00294590
rosa_rugosa Rorug01G0292500 Rorug01G0292600
rosa_samantha Rh1AG303000 Rh1BG266200 Rh1CG284400 Rh1DG296200
rosa_wichuraiana Rw1G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 223
AccBSI CCGCTC 1 cut(s) 36
AccII CGCG 1 cut(s) 532
AciI CCGC 8 cut(s) 13, 36, 59, 83, 122, 255, 604, 726
AclWI GGATC 1 cut(s) 741
AcuI CTGAAG 1 cut(s) 795
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 2 cut(s) 158, 653
AfiI CCNNNNNNNGG 1 cut(s) 245
AflII CTTAAG 1 cut(s) 262
AgsI TTSAA 4 cut(s) 76, 697, 829, 841
AjiI CACGTC 1 cut(s) 577
AjnI CCWGG 3 cut(s) 168, 197, 441
AluBI AGCT 4 cut(s) 48, 261, 470, 773
AluI AGCT 4 cut(s) 48, 261, 470, 773
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 879
AlwI GGATC 1 cut(s) 741
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 4 cut(s) 99, 276, 487, 713
ApeKI GCWGC 3 cut(s) 15, 677, 916
AscI GGCGCGCC 1 cut(s) 530
AspLEI GCGC 4 cut(s) 226, 532, 534, 744
AspS9I GGNCC 4 cut(s) 85, 100, 141, 172
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 3 cut(s) 85, 141, 172
BanI GGYRCC 1 cut(s) 223
BauI CACGAG 2 cut(s) 150, 557
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 2 cut(s) 2, 664
BccI CCATC 3 cut(s) 152, 445, 599
BceAI ACGGC 4 cut(s) 96, 502, 642, 755
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 3 cut(s) 170, 199, 443
BciVI GTATCC 1 cut(s) 904
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 879
BfaI CTAG 1 cut(s) 342
BfoI RGCGCY 2 cut(s) 227, 745
BfrI CTTAAG 1 cut(s) 262
BfuI GTATCC 1 cut(s) 904
BisI GCNGC 5 cut(s) 16, 60, 123, 678, 917
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 5 cut(s) 17, 61, 124, 679, 918
Bme1390I CCNGG 4 cut(s) 170, 199, 443, 656
Bme18I GGWCC 3 cut(s) 85, 141, 172
BmeT110I CYCGRG 1 cut(s) 102
BmgBI CACGTC 1 cut(s) 577
BmgT120I GGNCC 4 cut(s) 85, 100, 141, 172
BmiI GGNNCC 5 cut(s) 40, 87, 142, 225, 457
BmrFI CCNGG 4 cut(s) 170, 199, 443, 656
BmsI GCATC 2 cut(s) 225, 443
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 15
BpmI CTGGAG 4 cut(s) 181, 464, 659, 702
Bpu1102I GCTNAGC 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 730
BpuMI CCSGG 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 224
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 715
Bse3DI GCAATG 3 cut(s) 214, 369, 547
BseBI CCWGG 3 cut(s) 170, 199, 443
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 3 cut(s) 214, 369, 547
BseMII CTCAG 5 cut(s) 140, 217, 294, 621, 890
BsePI GCGCGC 1 cut(s) 530
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 2 cut(s) 2, 664
BseYI CCCAGC 1 cut(s) 863
Bsh1236I CGCG 1 cut(s) 532
BshFI GGCC 4 cut(s) 101, 278, 489, 715
BshNI GGYRCC 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 2 cut(s) 656, 716
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 5 cut(s) 151, 154, 344, 419, 509
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 879
BsmFI GGGAC 5 cut(s) 151, 154, 344, 419, 509
BsnI GGCC 4 cut(s) 101, 278, 489, 715
Bso31I GGTCTC 1 cut(s) 879
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 2 cut(s) 187, 733
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 8 cut(s) 13, 36, 59, 83, 122, 255, 604, 726
BspANI GGCC 4 cut(s) 101, 278, 489, 715
BspCNI CTCAG 5 cut(s) 139, 216, 295, 622, 889
BspFNI CGCG 1 cut(s) 532
BspLI GGNNCC 5 cut(s) 40, 87, 142, 225, 457
BspPI GGATC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 223
BspTI CTTAAG 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 879
BsrBI CCGCTC 1 cut(s) 36
BsrDI GCAATG 3 cut(s) 214, 369, 547
BsrFI RCCGGY 1 cut(s) 715
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 168
BssHII GCGCGC 1 cut(s) 530
BssMI GATC 2 cut(s) 187, 733
BssNI GRCGYC 1 cut(s) 224
BssSI CACGAG 2 cut(s) 150, 557
Bst2BI CACGAG 2 cut(s) 150, 557
Bst2UI CCWGG 3 cut(s) 170, 199, 443
Bst4CI ACNGT 2 cut(s) 802, 898
BstACI GRCGYC 1 cut(s) 224
BstAFI CTTAAG 1 cut(s) 262
BstC8I GCNNGC 4 cut(s) 276, 432, 532, 921
BstDEI CTNAG 6 cut(s) 49, 126, 203, 303, 630, 876
BstF5I GGATG 1 cut(s) 622
BstFNI CGCG 1 cut(s) 532
BstH2I RGCGCY 2 cut(s) 227, 745
BstHHI GCGC 4 cut(s) 226, 532, 534, 744
BstKTI GATC 2 cut(s) 190, 736
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 2 cut(s) 187, 733
BstMWI GCNNNNNNNGC 3 cut(s) 476, 536, 913
BstNI CCWGG 3 cut(s) 170, 199, 443
BstNSI RCATGY 1 cut(s) 923
BstPAI GACNNNNGTC 1 cut(s) 411
BstSCI CCNGG 4 cut(s) 168, 197, 441, 654
BstUI CGCG 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 2, 664
BstV2I GAAGAC 1 cut(s) 15
BsuI GTATCC 1 cut(s) 904
BsuRI GGCC 4 cut(s) 101, 278, 489, 715
BtrI CACGTC 1 cut(s) 577
BtsCI GGATG 1 cut(s) 622
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 4 cut(s) 134, 300, 613, 884
Cac8I GCNNGC 4 cut(s) 276, 432, 532, 921
CfoI GCGC 4 cut(s) 226, 532, 534, 744
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 4 cut(s) 85, 100, 141, 172
CseI GACGC 1 cut(s) 541
Csp6I GTAC 2 cut(s) 157, 652
CviAII CATG 4 cut(s) 482, 794, 920, 924
CviQI GTAC 2 cut(s) 157, 652
DdeI CTNAG 6 cut(s) 49, 126, 203, 303, 630, 876
DinI GGCGCC 1 cut(s) 225
DpnI GATC 2 cut(s) 189, 735
DpnII GATC 2 cut(s) 187, 733
EciI GGCGGA 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 879
Eco47I GGWCC 3 cut(s) 85, 141, 172
Eco57I CTGAAG 1 cut(s) 795
Eco88I CYCGRG 1 cut(s) 102
EcoRII CCWGG 3 cut(s) 168, 197, 441
EcoT22I ATGCAT 1 cut(s) 925
EgeI GGCGCC 1 cut(s) 225
EheI GGCGCC 1 cut(s) 225
FaeI CATG 4 cut(s) 485, 797, 923, 927
FaqI GGGAC 5 cut(s) 151, 154, 344, 419, 509
FatI CATG 4 cut(s) 481, 793, 919, 923
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 5 cut(s) 16, 60, 123, 678, 917
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 5 cut(s) 16, 60, 123, 678, 917
FspBI CTAG 1 cut(s) 342
GlaI GCGC 4 cut(s) 225, 531, 533, 743
GluI GCNGC 5 cut(s) 16, 60, 123, 678, 917
GsaI CCCAGC 1 cut(s) 867
GsuI CTGGAG 4 cut(s) 181, 464, 659, 702
HaeII RGCGCY 2 cut(s) 227, 745
HaeIII GGCC 4 cut(s) 101, 278, 489, 715
HapII CCGG 2 cut(s) 656, 716
HgaI GACGC 1 cut(s) 541
HhaI GCGC 4 cut(s) 226, 532, 534, 744
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 4 cut(s) 485, 797, 923, 927
Hin6I GCGC 4 cut(s) 224, 530, 532, 742
HinP1I GCGC 4 cut(s) 224, 530, 532, 742
HinfI GANTC 6 cut(s) 105, 113, 161, 497, 570, 600
HpaII CCGG 2 cut(s) 656, 716
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 3 cut(s) 556, 652, 721
Hpy188I TCNGA 2 cut(s) 137, 733
Hpy188III TCNNGA 4 cut(s) 94, 349, 391, 597
Hpy8I GTNNAC 3 cut(s) 556, 652, 721
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 5 cut(s) 91, 388, 404, 469, 801
HpyCH4III ACNGT 2 cut(s) 802, 898
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 3 cut(s) 18, 539, 923
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 536, 913
HpyF3I CTNAG 6 cut(s) 49, 126, 203, 303, 630, 876
HpySE526I ACGT 1 cut(s) 576
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 4 cut(s) 485, 797, 923, 927
HspAI GCGC 4 cut(s) 224, 530, 532, 742
KasI GGCGCC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 187, 733
LmnI GCTCC 3 cut(s) 258, 467, 585
Lsp1109I GCAGC 2 cut(s) 2, 664
LweI GCATC 2 cut(s) 225, 443
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 2 cut(s) 110, 416
MalI GATC 2 cut(s) 189, 735
MbiI CCGCTC 1 cut(s) 36
MboI GATC 2 cut(s) 187, 733
MboII GAAGA 5 cut(s) 20, 253, 370, 671, 779
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 2 cut(s) 336, 858
Mly113I GGCGCC 1 cut(s) 224
MlyI GAGTC 4 cut(s) 107, 114, 491, 564
Mph1103I ATGCAT 1 cut(s) 925
MseI TTAA 4 cut(s) 213, 263, 687, 783
MspA1I CMGCKG 2 cut(s) 15, 726
MspCI CTTAAG 1 cut(s) 262
MspI CCGG 2 cut(s) 656, 716
MspR9I CCNGG 4 cut(s) 170, 199, 443, 656
MvaI CCWGG 3 cut(s) 170, 199, 443
MvnI CGCG 1 cut(s) 532
MwoI GCNNNNNNNGC 3 cut(s) 476, 536, 913
NarI GGCGCC 1 cut(s) 224
NciI CCSGG 1 cut(s) 656
NdeII GATC 2 cut(s) 187, 733
NlaIII CATG 4 cut(s) 485, 797, 923, 927
NlaIV GGNNCC 5 cut(s) 40, 87, 142, 225, 457
NmeAIII GCCGAG 3 cut(s) 252, 393, 479
NmuCI GTSAC 2 cut(s) 110, 416
NsiI ATGCAT 1 cut(s) 925
NspI RCATGY 1 cut(s) 923
PaeI GCATGC 1 cut(s) 923
PalAI GGCGCGCC 1 cut(s) 530
PauI GCGCGC 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 2 cut(s) 161, 600
Pfl23II CGTACG 1 cut(s) 156
PkrI GCNGC 5 cut(s) 17, 61, 124, 679, 918
PleI GAGTC 4 cut(s) 107, 113, 491, 564
PluTI GGCGCC 1 cut(s) 227
PpsI GAGTC 4 cut(s) 107, 113, 491, 564
PshAI GACNNNNGTC 1 cut(s) 411
Psp6I CCWGG 3 cut(s) 168, 197, 441
PspFI CCCAGC 1 cut(s) 863
PspGI CCWGG 3 cut(s) 168, 197, 441
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 5 cut(s) 40, 87, 142, 225, 457
PspPI GGNCC 4 cut(s) 85, 100, 141, 172
PteI GCGCGC 1 cut(s) 530
RsaI GTAC 2 cut(s) 158, 653
RsaNI GTAC 2 cut(s) 157, 652
SaqAI TTAA 4 cut(s) 213, 263, 687, 783
SatI GCNGC 5 cut(s) 16, 60, 123, 678, 917
Sau3AI GATC 2 cut(s) 187, 733
Sau96I GGNCC 4 cut(s) 85, 100, 141, 172
SchI GAGTC 4 cut(s) 107, 114, 491, 564
ScrFI CCNGG 4 cut(s) 170, 199, 443, 656
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 2 cut(s) 225, 443
SfoI GGCGCC 1 cut(s) 225
SgsI GGCGCGCC 1 cut(s) 530
SinI GGWCC 3 cut(s) 85, 141, 172
SmlI CTYRAG 2 cut(s) 262, 709
SmoI CTYRAG 2 cut(s) 262, 709
SphI GCATGC 1 cut(s) 923
Sse9I AATT 2 cut(s) 336, 858
SsiI CCGC 8 cut(s) 13, 36, 59, 83, 122, 255, 604, 726
SspDI GGCGCC 1 cut(s) 223
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 4 cut(s) 168, 197, 441, 654
TaaI ACNGT 2 cut(s) 802, 898
TaiI ACGT 1 cut(s) 579
TaqI TCGA 5 cut(s) 93, 233, 543, 573, 598
TasI AATT 2 cut(s) 336, 858
TauI GCSGC 2 cut(s) 62, 125
TfiI GAWTC 2 cut(s) 161, 600
Tru1I TTAA 4 cut(s) 213, 263, 687, 783
Tru9I TTAA 4 cut(s) 213, 263, 687, 783
TscAI CASTG 4 cut(s) 134, 307, 613, 884
TseFI GTSAC 2 cut(s) 110, 416
TseI GCWGC 3 cut(s) 15, 677, 916
Tsp45I GTSAC 2 cut(s) 110, 416
TspDTI ATGAA 4 cut(s) 275, 780, 782, 858
TspGWI ACGGA 2 cut(s) 169, 398
TspRI CASTG 4 cut(s) 134, 307, 613, 884
Vha464I CTTAAG 1 cut(s) 262
VpaK11BI GGWCC 3 cut(s) 85, 141, 172
XceI RCATGY 1 cut(s) 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XspI CTAG 1 cut(s) 342
Zsp2I ATGCAT 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.