AT5G06480
ERF Family

MD-2-related lipid recognition domain-containing protein ML domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009073
Physical Location & Seq
Reverse (-)
1975947 .. 1977377
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06480.1

Sequence Viewer

Length: 462 bp
ATGTCGAGGATCTTCGCCATTCTTCCTCTCGCTGCTGTTTTTCTTCTCCTCCTTGTTTCACCGATTGTAGCCATCGATGTCCATTACTGTGAAGAGAACGCAGAGTATGAAGTTAAAGTGAAAGAGGTTGATATATCTCCTAATCCTATAGCACCAGGCGAGCCAGCTACTTTCACCATCTCTGCCAACACAGGCCGTGAGATTTCGTTTGGGAAGCTGGTGATTGAAGTTTCATACTTTGGATGGCATGTTCACTCTGAGACTCATGACCTCTGCACTGAGACTAGTTGTCCTATACAAACCGGAGATTTCTTGGTTGCACACTCTCAAGTTCTTCCTGGTTATACTCCTCCTGGTTCATACTTGCTGAAAATGAAGATGCTTGATGCAAAGAAGAAGGAGTTAACATGCATCAAATTCTCGTTCGACATTGGACTCAGAGCATCTGTGGCTGACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E1_DerP2_DerF2

Protein Analysis

153

Amino Acids

16.83

Weight (kDa)

5.28

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_DerP2_DerF2 PF02221 25 - 143 3e-26 ML domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 416
AgsI TTSAA 1 cut(s) 227
AhdI GACNNNNNGTC 1 cut(s) 288
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 3 cut(s) 154, 337, 352
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
AluBI AGCT 2 cut(s) 167, 217
AluI AGCT 2 cut(s) 167, 217
Alw26I GTCTC 2 cut(s) 254, 275
AlwI GGATC 1 cut(s) 17
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 416
AsuHPI GGTGA 3 cut(s) 51, 166, 232
BbvI GCAGC 1 cut(s) 19
BccI CCATC 3 cut(s) 80, 185, 237
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 3 cut(s) 156, 339, 354
BcoDI GTCTC 2 cut(s) 254, 275
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 147
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 3 cut(s) 156, 339, 354
BmeRI GACNNNNNGTC 1 cut(s) 288
BmrFI CCNGG 3 cut(s) 156, 339, 354
BmsI GCATC 4 cut(s) 369, 376, 420, 452
BpuEI CTTGAG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 302
BseBI CCWGG 3 cut(s) 156, 339, 354
BseCI ATCGAT 1 cut(s) 75
BseGI GGATG 1 cut(s) 248
BseMII CTCAG 3 cut(s) 249, 270, 451
BseRI GAGGAG 2 cut(s) 38, 339
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 259
BshFI GGCC 1 cut(s) 195
BshVI ATCGAT 1 cut(s) 75
BsiSI CCGG 1 cut(s) 303
BsmAI GTCTC 2 cut(s) 254, 275
BsnI GGCC 1 cut(s) 195
Bsp143I GATC 1 cut(s) 9
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 3 cut(s) 250, 271, 450
BspDI ATCGAT 1 cut(s) 75
BspHI TCATGA 1 cut(s) 265
BspPI GGATC 1 cut(s) 17
BssMI GATC 1 cut(s) 9
Bst2UI CCWGG 3 cut(s) 156, 339, 354
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 161, 165
BstDEI CTNAG 3 cut(s) 258, 279, 437
BstF5I GGATG 1 cut(s) 248
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 2 cut(s) 254, 275
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 3 cut(s) 156, 339, 354
BstNSI RCATGY 2 cut(s) 251, 411
BstSCI CCNGG 3 cut(s) 154, 337, 352
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 1 cut(s) 195
BsuTUI ATCGAT 1 cut(s) 75
BtsCI GGATG 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 161, 165
CciI TCATGA 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 75
CviAII CATG 3 cut(s) 248, 266, 408
CviJI RGCY 6 cut(s) 71, 163, 167, 195, 217, 452
CviKI_1 RGCY 6 cut(s) 71, 163, 167, 195, 217, 452
DdeI CTNAG 3 cut(s) 258, 279, 437
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
DriI GACNNNNNGTC 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 87
Eam1105I GACNNNNNGTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 87
EcoRII CCWGG 3 cut(s) 154, 337, 352
EcoT22I ATGCAT 1 cut(s) 413
FaeI CATG 3 cut(s) 251, 269, 411
FatI CATG 3 cut(s) 247, 265, 407
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 285
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 1 cut(s) 195
HapII CCGG 1 cut(s) 303
Hin1II CATG 3 cut(s) 251, 269, 411
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HinfI GANTC 2 cut(s) 262, 435
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 1 cut(s) 303
HphI GGTGA 3 cut(s) 51, 166, 232
Hpy166II GTNNAC 2 cut(s) 253, 405
Hpy188I TCNGA 2 cut(s) 259, 440
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 2 cut(s) 253, 405
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4V TGCA 4 cut(s) 276, 320, 389, 411
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 3 cut(s) 258, 279, 437
Hsp92II CATG 3 cut(s) 251, 269, 411
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 1 cut(s) 9
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 4 cut(s) 369, 376, 420, 452
MaeI CTAG 1 cut(s) 285
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 7 cut(s) 4, 14, 35, 104, 326, 388, 406
MflI RGATCY 1 cut(s) 9
MluCI AATT 1 cut(s) 416
MlyI GAGTC 2 cut(s) 256, 429
MnlI CCTC 5 cut(s) 36, 59, 118, 281, 360
Mph1103I ATGCAT 1 cut(s) 413
MseI TTAA 2 cut(s) 114, 404
MslI CAYNNNNRTG 1 cut(s) 87
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 3 cut(s) 156, 339, 354
MvaI CCWGG 3 cut(s) 156, 339, 354
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeII GATC 1 cut(s) 9
NlaIII CATG 3 cut(s) 251, 269, 411
NsiI ATGCAT 1 cut(s) 413
NspI RCATGY 2 cut(s) 251, 411
PagI TCATGA 1 cut(s) 265
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 2 cut(s) 256, 429
PpsI GAGTC 2 cut(s) 256, 429
Psp6I CCWGG 3 cut(s) 154, 337, 352
PspGI CCWGG 3 cut(s) 154, 337, 352
PsuI RGATCY 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 87
SaqAI TTAA 2 cut(s) 114, 404
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 1 cut(s) 9
SchI GAGTC 2 cut(s) 256, 429
ScrFI CCNGG 3 cut(s) 156, 339, 354
SetI ASST 4 cut(s) 129, 169, 219, 273
SfaNI GCATC 4 cut(s) 369, 376, 420, 452
SfcI CTRYAG 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SpeI ACTAGT 1 cut(s) 284
Sse9I AATT 1 cut(s) 416
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 154, 337, 352
TaaI ACNGT 1 cut(s) 89
TaqI TCGA 3 cut(s) 5, 75, 426
TasI AATT 1 cut(s) 416
Tru1I TTAA 2 cut(s) 114, 404
Tru9I TTAA 2 cut(s) 114, 404
TscAI CASTG 1 cut(s) 283
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 4 cut(s) 123, 222, 348, 389
TspRI CASTG 1 cut(s) 283
XapI RAATTY 1 cut(s) 416
XceI RCATGY 2 cut(s) 251, 411
XspI CTAG 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.