MD12G1042800.v1.1
ERF Family

Phosphatidylglycerol phosphatidylinositol transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009073
Physical Location & Seq
Forward (+)
4797510 .. 4800235
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1042800.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGCTCTTCAGTTGAAGTCCATCGTCCCCCTCATCATCCTAATTTCTCTCTGTCTGGATGCCCCTCGCGTCCAAGCCAAGGACGTCAACTACTGCGATAAGAAGGTTGACTATGATGTCAAGGTCTCTGCGGTTGATATTAATCCTTACCCCGTGGCAAGAGGCAAGCCTGCCAGCTTCAGCATTTCTGCAACTGCAGGTGCATCAATCTCTGGAGGGAAATTGGTGATTGAGGTTTCATACTTTGGTTGGCACATCCACAGTGAGACTCATGACCTTTGCGCAGAAACATCTTGCCCTGTTTCAACTGGTGATTTTGTGATTGCCCACTCGCAAGATTTACCAGGATATACTCCACCTGGTTCGTACTCTCTTAAAATGAGGCTGTTTGACGGAAACAACCATGAGCTGACATGCATTGCCTTCGATTTTGACATCGGGTTTGCATCATCTGTGGCTGATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E1_DerP2_DerF2

Protein Analysis

156

Amino Acids

16.64

Weight (kDa)

5.15

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_DerP2_DerF2 PF02221 28 - 146 7.4e-25 ML domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 188
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 1 cut(s) 87
Acc16I TGCGCA 1 cut(s) 283
Acc36I ACCTGC 1 cut(s) 188
AccII CGCG 1 cut(s) 69
AciI CCGC 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 2 cut(s) 16, 306
AjnI CCWGG 2 cut(s) 343, 358
AluBI AGCT 2 cut(s) 177, 409
AluI AGCT 2 cut(s) 177, 409
Alw26I GTCTC 2 cut(s) 130, 260
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 1 cut(s) 141
AspLEI GCGC 1 cut(s) 284
AsuHPI GGTGA 2 cut(s) 238, 323
BccI CCATC 1 cut(s) 29
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 2 cut(s) 345, 360
BcoDI GTCTC 2 cut(s) 130, 260
BfmI CTRYAG 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 345, 360
BmrFI CCNGG 2 cut(s) 345, 360
BmsI GCATC 3 cut(s) 49, 212, 455
BpmI CTGGAG 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 78, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 345, 360
BseDI CCNNGG 2 cut(s) 78, 153
BseGI GGATG 3 cut(s) 36, 64, 255
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMI GCAATG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 313
Bsh1236I CGCG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 130, 260
BsmFI GGGAC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 130
BspACI CCGC 1 cut(s) 131
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 78, 153
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 345, 360
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 167, 171, 175
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 36, 64, 255
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 284
BstMAI GTCTC 2 cut(s) 130, 260
BstNI CCWGG 2 cut(s) 345, 360
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 343, 358
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 36, 64, 255
BtsIMutI CAGTG 1 cut(s) 268
BveI ACCTGC 1 cut(s) 188
Cac8I GCNNGC 3 cut(s) 167, 171, 175
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 284
CseI GACGC 1 cut(s) 58
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 1 cut(s) 367
CviAII CATG 3 cut(s) 272, 404, 414
CviJI RGCY 7 cut(s) 5, 77, 169, 177, 385, 409, 458
CviKI_1 RGCY 7 cut(s) 5, 77, 169, 177, 385, 409, 458
CviQI GTAC 1 cut(s) 367
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 163
EcoRII CCWGG 2 cut(s) 343, 358
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 3 cut(s) 275, 407, 417
FaiI YATR 6 cut(s) 114, 241, 273, 351, 405, 415
FaqI GGGAC 1 cut(s) 11
FatI CATG 3 cut(s) 271, 403, 413
FokI GGATG 3 cut(s) 23, 71, 242
FspI TGCGCA 1 cut(s) 283
GlaI GCGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 234
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 275, 407, 417
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 88, 109
HindII GTYRAC 2 cut(s) 88, 109
HinfI GANTC 1 cut(s) 268
HphI GGTGA 2 cut(s) 238, 323
Hpy166II GTNNAC 2 cut(s) 88, 109
Hpy188III TCNNGA 3 cut(s) 56, 213, 272
Hpy8I GTNNAC 2 cut(s) 88, 109
HpyAV CCTTC 2 cut(s) 97, 433
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 5 cut(s) 191, 197, 203, 417, 446
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 275, 407, 417
HspAI GCGC 1 cut(s) 282
LguI GCTCTTC 1 cut(s) 12
LweI GCATC 3 cut(s) 49, 212, 455
MabI ACCWGGT 1 cut(s) 358
MaeII ACGT 1 cut(s) 84
MluCI AATT 2 cut(s) 42, 221
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 6 cut(s) 41, 75, 155, 209, 226, 375
Mph1103I ATGCAT 1 cut(s) 419
MseI TTAA 3 cut(s) 141, 375, 466
MspR9I CCNGG 2 cut(s) 345, 360
MvaI CCWGG 2 cut(s) 345, 360
MvnI CGCG 1 cut(s) 69
NlaIII CATG 3 cut(s) 275, 407, 417
NsbI TGCGCA 1 cut(s) 283
NsiI ATGCAT 1 cut(s) 419
NspI RCATGY 1 cut(s) 417
PagI TCATGA 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 12
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PshBI ATTAAT 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 343, 358
PspGI CCWGG 2 cut(s) 343, 358
PstI CTGCAG 1 cut(s) 199
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 141, 375, 466
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 345, 360
SetI ASST 9 cut(s) 87, 108, 126, 179, 202, 237, 279, 361, 411
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 3 cut(s) 49, 212, 455
SfcI CTRYAG 1 cut(s) 195
Sse9I AATT 2 cut(s) 42, 221
SsiI CCGC 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 343, 358
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 426
TasI AATT 2 cut(s) 42, 221
Tru1I TTAA 3 cut(s) 141, 375, 466
Tru9I TTAA 3 cut(s) 141, 375, 466
TscAI CASTG 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 1 cut(s) 268
VspI ATTAAT 1 cut(s) 141
XceI RCATGY 1 cut(s) 417
ZraI GACGTC 1 cut(s) 85
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.