pycom12g03700
ERF Family

Phosphatidylglycerol phosphatidylinositol transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009073
Physical Location & Seq
Forward (+)
3593833 .. 3596719
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03700.1

Sequence Viewer

Length: 579 bp
ATGGCTCTTCAGTTGAAGTCCATCGTCCCCCTCATCATCCTAATTTCTCTCTGTCTGGTTGCCCCTCTCATCCACGCCAAGGACGTCAACTACTGCGATAAGAAGGCTGACTATGATGTCAAGGTCTCTGCGGTTGACATTAATCCTTACCCTGTGGCGAGAGGCAAGCCTGCCAGCTTCAGTATTTCTGCAACTGCAGGTGCATCAATCTCTGGAGGGAAATTGGTGATTGAGGTTTCATACTTTGGTTGGCACATCCACAGTGAGACTCATGACCTTTGCGCGGAAACATCTTGCCCTGTTTCAACTGGTGATTTTGTGATTGCCCACTCACAAGATTTACCCGGATATACTCCACCTGGTTCGTACTCTCTTAAAATGAGGCTGTATGACGGAAACAACCATGAGCTGACATGCATTGCCTTTGATTTTGACATCGGGTTTGCATCATCTGTGGCTGATAGTCTTGGTGGTAAAATTCTTCCCGATGTCAGAGGAGGTCTCAAGTTTGGAGTCTTCACTCTTGTATTGATGTCCGAAACACAATTGGATTGCAGACGCCTGTCACGGTACCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E1_DerP2_DerF2

Protein Analysis

193

Amino Acids

20.71

Weight (kDa)

6.03

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_DerP2_DerF2 PF02221 28 - 146 1e-25 ML domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 188
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 188
Acc65I GGTACC 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 570
AccII CGCG 1 cut(s) 284
AciI CCGC 2 cut(s) 131, 284
AcsI RAATTY 1 cut(s) 477
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 2 cut(s) 84, 559
AfaI GTAC 2 cut(s) 368, 572
AfiI CCNNNNNNNGG 2 cut(s) 79, 283
AgsI TTSAA 2 cut(s) 16, 306
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 2 cut(s) 177, 409
AluI AGCT 2 cut(s) 177, 409
Alw26I GTCTC 3 cut(s) 130, 260, 506
ApoI RAATTY 1 cut(s) 477
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 1 cut(s) 141
Asp718I GGTACC 1 cut(s) 570
AspLEI GCGC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 238, 323
BanI GGYRCC 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 508
BccI CCATC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 360
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 3 cut(s) 130, 260, 506
BfaI CTAG 1 cut(s) 577
BfmI CTRYAG 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 345, 360
BmiI GGNNCC 1 cut(s) 572
BmrFI CCNGG 2 cut(s) 345, 360
BmsI GCATC 2 cut(s) 212, 455
BoxI GACNNNNGTC 1 cut(s) 562
BpiI GAAGAC 1 cut(s) 508
BplI GAGNNNNNCTC 2 cut(s) 486, 518
BpmI CTGGAG 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 345
BsaHI GRCGYC 2 cut(s) 84, 559
BsaI GGTCTC 2 cut(s) 130, 506
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 283
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 3 cut(s) 36, 69, 255
BseLI CCNNNNNNNGG 2 cut(s) 79, 283
BseMI GCAATG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 284
BshNI GGYRCC 1 cut(s) 570
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 2 cut(s) 79, 283
BsmAI GTCTC 3 cut(s) 130, 260, 506
BsmFI GGGAC 1 cut(s) 11
Bso31I GGTCTC 2 cut(s) 130, 506
BspACI CCGC 2 cut(s) 131, 284
BspFNI CGCG 1 cut(s) 284
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 572
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 570
BspTNI GGTCTC 2 cut(s) 130, 506
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 78
BssNI GRCGYC 2 cut(s) 84, 559
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 2 cut(s) 263, 570
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 2 cut(s) 84, 559
BstC8I GCNNGC 3 cut(s) 167, 171, 175
BstF5I GGATG 3 cut(s) 36, 69, 255
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 284
BstMAI GTCTC 3 cut(s) 130, 260, 506
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 1 cut(s) 417
BstPAI GACNNNNGTC 1 cut(s) 562
BstSCI CCNGG 2 cut(s) 343, 358
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 1 cut(s) 284
BstV2I GAAGAC 1 cut(s) 508
BtsCI GGATG 3 cut(s) 36, 69, 255
BtsIMutI CAGTG 1 cut(s) 268
BveI ACCTGC 1 cut(s) 188
Cac8I GCNNGC 3 cut(s) 167, 171, 175
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 284
CseI GACGC 1 cut(s) 567
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 2 cut(s) 367, 571
CviAII CATG 3 cut(s) 272, 404, 414
CviJI RGCY 7 cut(s) 5, 107, 169, 177, 385, 409, 458
CviKI_1 RGCY 7 cut(s) 5, 107, 169, 177, 385, 409, 458
CviQI GTAC 2 cut(s) 367, 571
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 2 cut(s) 130, 506
Eco57I CTGAAG 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 3 cut(s) 275, 407, 417
FaiI YATR 7 cut(s) 114, 241, 273, 351, 390, 405, 415
FaqI GGGAC 1 cut(s) 11
FatI CATG 3 cut(s) 271, 403, 413
FokI GGATG 3 cut(s) 23, 56, 242
FspBI CTAG 1 cut(s) 577
GlaI GCGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 234
HapII CCGG 1 cut(s) 345
HgaI GACGC 1 cut(s) 567
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 2 cut(s) 84, 559
Hin1II CATG 3 cut(s) 275, 407, 417
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 88, 136
HindII GTYRAC 2 cut(s) 88, 136
HinfI GANTC 2 cut(s) 268, 513
HpaII CCGG 1 cut(s) 345
HphI GGTGA 2 cut(s) 238, 323
Hpy166II GTNNAC 2 cut(s) 88, 136
Hpy188I TCNGA 2 cut(s) 494, 538
Hpy188III TCNNGA 3 cut(s) 213, 272, 485
Hpy8I GTNNAC 2 cut(s) 88, 136
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 263, 570
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 6 cut(s) 191, 197, 203, 417, 446, 555
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 2 cut(s) 84, 559
Hsp92II CATG 3 cut(s) 275, 407, 417
HspAI GCGC 1 cut(s) 282
KpnI GGTACC 1 cut(s) 574
LguI GCTCTTC 1 cut(s) 12
LweI GCATC 2 cut(s) 212, 455
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 1 cut(s) 577
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 564
MboII GAAGA 2 cut(s) 473, 508
MfeI CAATTG 1 cut(s) 545
MluCI AATT 4 cut(s) 42, 221, 477, 545
MlyI GAGTC 2 cut(s) 262, 522
MnlI CCTC 8 cut(s) 41, 75, 155, 209, 226, 375, 488, 491
Mph1103I ATGCAT 1 cut(s) 419
MseI TTAA 2 cut(s) 141, 375
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 345, 360
MunI CAATTG 1 cut(s) 545
MvaI CCWGG 1 cut(s) 360
MvnI CGCG 1 cut(s) 284
NciI CCSGG 1 cut(s) 345
NlaIII CATG 3 cut(s) 275, 407, 417
NlaIV GGNNCC 1 cut(s) 572
NmuCI GTSAC 1 cut(s) 564
NsiI ATGCAT 1 cut(s) 419
NspI RCATGY 1 cut(s) 417
PagI TCATGA 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PleI GAGTC 2 cut(s) 262, 521
PpsI GAGTC 2 cut(s) 262, 521
PshAI GACNNNNGTC 1 cut(s) 562
PshBI ATTAAT 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 572
PstI CTGCAG 1 cut(s) 199
RsaI GTAC 2 cut(s) 368, 572
RsaNI GTAC 2 cut(s) 367, 571
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 141, 375
SchI GAGTC 2 cut(s) 262, 522
ScrFI CCNGG 2 cut(s) 345, 360
SetI ASST 9 cut(s) 87, 126, 179, 202, 237, 279, 361, 411, 502
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 2 cut(s) 212, 455
SfcI CTRYAG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 4 cut(s) 42, 221, 477, 545
SsiI CCGC 2 cut(s) 131, 284
SspMI CTAG 1 cut(s) 577
StyD4I CCNGG 2 cut(s) 343, 358
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 2 cut(s) 263, 570
TaiI ACGT 1 cut(s) 87
TasI AATT 4 cut(s) 42, 221, 477, 545
Tru1I TTAA 2 cut(s) 141, 375
Tru9I TTAA 2 cut(s) 141, 375
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 1 cut(s) 268
VspI ATTAAT 1 cut(s) 141
XapI RAATTY 1 cut(s) 477
XceI RCATGY 1 cut(s) 417
XspI CTAG 1 cut(s) 577
ZraI GACGTC 1 cut(s) 85
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.